AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0702890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0702890 MtrunA17_Chr8g0380901 98.347 121 2 0 1 121 1 121 1.25e-86 247
Msa0702890 MtrunA17_Chr8g0380711 77.869 122 26 1 1 121 1 122 6.34e-57 172
Msa0702890 MtrunA17_Chr3g0125791 60.800 125 44 1 1 120 1 125 5.25e-51 157
Msa0702890 MtrunA17_Chr3g0125761 58.268 127 46 2 1 121 1 126 1.84e-49 153
Msa0702890 MtrunA17_Chr2g0301431 55.645 124 51 1 1 120 1 124 1.33e-45 144
Msa0702890 MtrunA17_Chr2g0323021 40.000 115 64 4 9 119 6 119 2.82e-24 92.8
Msa0702890 MtrunA17_Chr7g0253681 38.462 117 68 3 8 120 7 123 3.40e-22 85.5
Msa0702890 MtrunA17_Chr7g0253701 36.066 122 74 3 3 120 1 122 3.64e-21 82.8
Msa0702890 MtrunA17_Chr7g0253671 36.066 122 74 3 3 120 1 122 3.64e-21 82.8
Msa0702890 MtrunA17_Chr1g0177341 39.316 117 67 3 7 119 4 120 6.08e-21 82.4
Msa0702890 MtrunA17_Chr1g0177331 39.316 117 67 3 7 119 4 120 6.08e-21 82.4
Msa0702890 MtrunA17_Chr7g0253731 38.525 122 68 4 1 118 1 119 1.37e-20 81.6
Msa0702890 MtrunA17_Chr8g0335531 42.241 116 59 5 11 119 5 119 1.79e-20 81.6
Msa0702890 MtrunA17_Chr7g0253761 39.837 123 69 3 1 120 1 121 3.02e-20 80.9
Msa0702890 MtrunA17_Chr1g0205701 33.621 116 68 3 11 119 8 121 6.97e-20 80.1
Msa0702890 MtrunA17_Chr7g0253641 39.344 122 69 3 1 119 1 120 1.21e-19 80.5
Msa0702890 MtrunA17_Chr5g0394321 33.043 115 72 2 10 119 8 122 5.50e-19 77.8
Msa0702890 MtrunA17_Chr1g0211101 36.752 117 68 4 8 120 15 129 5.86e-19 77.8
Msa0702890 MtrunA17_Chr8g0335571 39.655 116 62 5 11 119 5 119 5.87e-19 78.2
Msa0702890 MtrunA17_Chr4g0064041 40.000 100 53 2 27 119 190 289 7.49e-18 77.4
Msa0702890 MtrunA17_Chr4g0064041 36.441 118 67 3 9 119 11 127 1.69e-15 70.9
Msa0702890 MtrunA17_Chr6g0481491 35.106 94 57 3 28 118 28 120 7.19e-17 72.8
Msa0702890 MtrunA17_Chr2g0306461 32.258 124 77 3 1 118 1 123 9.42e-17 72.4
Msa0702890 MtrunA17_Chr6g0483981 40.777 103 52 2 25 118 24 126 1.15e-16 74.7
Msa0702890 MtrunA17_Chr7g0253631 38.525 122 70 3 1 119 1 120 1.31e-15 70.1
Msa0702890 MtrunA17_Chr3g0132701 38.596 114 64 5 11 118 7 120 1.72e-15 69.3
Msa0702890 MtrunA17_Chr6g0459441 36.000 100 59 4 25 120 23 121 2.29e-15 68.6
Msa0702890 MtrunA17_Chr3g0132431 39.823 113 58 6 11 116 7 116 5.20e-15 67.4
Msa0702890 MtrunA17_Chr6g0453801 30.952 126 79 3 1 120 8 131 1.92e-14 65.9
Msa0702890 MtrunA17_Chr3g0132511 37.719 114 65 5 11 118 7 120 2.31e-14 66.6
Msa0702890 MtrunA17_Chr8g0387804 33.621 116 63 4 13 120 18 127 8.74e-14 63.2
Msa0702890 MtrunA17_Chr6g0453921 34.454 119 73 3 7 120 16 134 4.19e-13 62.4
Msa0702890 MtrunA17_Chr3g0132411 36.752 117 68 5 7 118 42 157 1.40e-12 60.8
Msa0702890 MtrunA17_Chr1g0150131 31.667 120 73 3 7 118 5 123 1.52e-12 61.2
Msa0702890 MtrunA17_Chr4g0064031 33.981 103 56 4 24 115 24 125 6.06e-12 58.9
Msa0702890 MtrunA17_Chr8g0360371 31.304 115 74 2 9 118 5 119 1.10e-11 60.1
Msa0702890 MtrunA17_Chr4g0001571 31.250 96 64 1 25 118 26 121 2.73e-11 57.4
Msa0702890 MtrunA17_Chr6g0453791 32.292 96 62 2 28 120 31 126 8.01e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0702890 AT2G02850.1 60.156 128 43 1 1 120 1 128 1.78e-52 161
Msa0702890 AT3G17675.1 36.066 122 73 3 5 121 7 128 5.73e-22 84.3
Msa0702890 AT1G17800.1 41.176 119 64 5 8 120 18 136 6.69e-21 82.0
Msa0702890 AT2G32300.1 39.796 98 56 2 25 119 23 120 2.85e-20 83.2
Msa0702890 AT2G32300.2 39.796 98 56 2 25 119 53 150 4.52e-20 83.2
Msa0702890 AT3G60270.1 42.857 98 52 3 25 119 23 119 6.01e-20 80.9
Msa0702890 AT3G27200.1 36.975 119 70 4 7 121 5 122 3.83e-19 78.6
Msa0702890 AT5G26330.1 35.652 115 64 4 12 119 9 120 2.32e-18 76.6
Msa0702890 AT3G60280.1 44.898 98 48 4 25 118 21 116 4.07e-18 76.6
Msa0702890 AT2G44790.1 43.137 102 48 5 23 119 27 123 1.21e-16 72.4
Msa0702890 AT2G31050.1 33.071 127 74 4 1 119 1 124 1.36e-16 72.4
Msa0702890 AT2G26720.1 33.333 99 62 2 26 120 27 125 4.42e-16 71.2
Msa0702890 AT5G07475.1 34.821 112 69 3 11 119 13 123 7.58e-16 70.1
Msa0702890 AT2G25060.1 33.613 119 67 3 8 118 15 129 2.26e-13 63.5
Msa0702890 AT3G20570.1 31.858 113 70 3 12 118 15 126 2.51e-13 63.9
Msa0702890 AT4G12880.1 30.081 123 79 4 3 118 1 123 2.81e-12 59.7
Msa0702890 AT1G22480.1 38.793 116 61 5 11 121 4 114 8.63e-12 59.3
Msa0702890 AT4G31840.1 31.667 120 70 4 11 118 6 125 8.87e-12 59.3
Msa0702890 AT1G48940.1 32.308 130 66 8 6 120 3 125 1.14e-11 58.9
Msa0702890 AT3G18590.1 31.496 127 72 7 6 120 3 126 1.20e-11 59.3
Msa0702890 AT4G27520.1 33.913 115 67 3 9 118 16 126 1.32e-11 60.5
Msa0702890 AT5G53870.1 30.952 126 71 5 7 118 2 125 6.29e-11 58.5
Msa0702890 AT2G27035.1 29.661 118 77 3 8 120 8 124 7.06e-11 56.6

Find 68 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATCCACTTATTTAATTTA+AGG 0.058031 4_4:+74734892 None:intergenic
ATTTACCACTGTTATTATTT+TGG 0.148598 4_4:-74734727 Msa0702890:three_prime_UTR
GATTTGAAGGTAAGTGAATT+TGG 0.221004 4_4:+74735750 None:intergenic
AAATATAAACATAATGAGTT+TGG 0.254292 4_4:-74735911 Msa0702890:five_prime_UTR
AAGTTGAACACTACATTATT+AGG 0.282780 4_4:+74735017 None:intergenic
TAAATACTTCTTCATATTGC+AGG 0.322954 4_4:+74735856 None:intergenic
AGGCCAACCAACAGTGTTAA+AGG 0.339711 4_4:+74735577 None:intergenic
ACCTACACTGTTGGAGGTCC+TGG 0.340570 4_4:-74735608 Msa0702890:CDS
CACCCATTAAGATTTCTTGC+AGG 0.368254 4_4:-74735820 Msa0702890:five_prime_UTR
TTTGTGGGTCACTGCGAGTC+TGG 0.369480 4_4:-74735091 Msa0702890:CDS
TAAAAGTTAAGCAGCATTGA+TGG 0.375502 4_4:+74735057 None:intergenic
GTAGTTCCCTTCAAAACAAT+TGG 0.383981 4_4:+74734761 None:intergenic
AGGCCTGCAAGAAATCTTAA+TGG 0.392067 4_4:+74735817 None:intergenic
TTATCCTTAAATTAAATAAG+TGG 0.400701 4_4:-74734896 Msa0702890:three_prime_UTR
CTACTTCATTTGCAACTTTG+TGG 0.404030 4_4:-74735107 Msa0702890:CDS
AATATAAACATAATGAGTTT+GGG 0.430122 4_4:-74735910 Msa0702890:five_prime_UTR
TGTGAAAGAAAGGGATTTGA+AGG 0.430605 4_4:+74735737 None:intergenic
CTACTATATGTTAGTCATCA+TGG 0.431538 4_4:-74734846 Msa0702890:three_prime_UTR
GCTAGAAAACTGTGAAAGAA+AGG 0.441460 4_4:+74735727 None:intergenic
TACTTCATTTGCAACTTTGT+GGG 0.443056 4_4:-74735106 Msa0702890:CDS
GGAGCAAAAGTGTATAGGTC+AGG 0.453219 4_4:-74735163 Msa0702890:CDS
TAACACTGTTGGTTGGCCTA+AGG 0.454454 4_4:-74735573 Msa0702890:CDS
CTAGTAGAGAAACATGGCTC+TGG 0.459629 4_4:-74735699 Msa0702890:exon
AGGATCAGATAAGGCTTGCT+AGG 0.465568 4_4:-74735138 Msa0702890:CDS
AACACTGTTGGTTGGCCTAA+GGG 0.479850 4_4:-74735572 Msa0702890:CDS
GGACCTTTAACACTGTTGGT+TGG 0.480078 4_4:-74735580 Msa0702890:CDS
TTGGGCAGACCCACGTGTCT+TGG 0.480551 4_4:-74735892 Msa0702890:five_prime_UTR
CTAGAAAACTGTGAAAGAAA+GGG 0.482984 4_4:+74735728 None:intergenic
AGTACACATGCATAAAGAAC+AGG 0.484146 4_4:+74734939 None:intergenic
AGAAACATGGCTCTGGGAAG+AGG 0.491666 4_4:-74735692 Msa0702890:exon
GGCCTGCAAGAAATCTTAAT+GGG 0.503092 4_4:+74735818 None:intergenic
GAATTTGGAATGATGTGATA+AGG 0.505362 4_4:+74735765 None:intergenic
GTGTTAAAGGTCCAACCTCC+AGG 0.508532 4_4:+74735590 None:intergenic
ATATGATTTCTGGGTCTCTC+CGG 0.514100 4_4:-74734799 Msa0702890:three_prime_UTR
GCTGCTACCTACACTGTTGG+AGG 0.517016 4_4:-74735614 Msa0702890:CDS
CAAGAGGAGCAAAAGTGTAT+AGG 0.522207 4_4:-74735168 Msa0702890:CDS
GCCACCACATTGTGAGCTGA+TGG 0.525344 4_4:+74735226 None:intergenic
CATGCTGCTACCTACACTGT+TGG 0.528640 4_4:-74735617 Msa0702890:CDS
CTGTTGGAGGTCCTGGAGGT+TGG 0.534102 4_4:-74735601 Msa0702890:CDS
AAGAAATCTTAATGGGTGGT+TGG 0.538997 4_4:+74735825 None:intergenic
GGTTGGACCTTTAACACTGT+TGG 0.539100 4_4:-74735584 Msa0702890:CDS
TTTAGGGCTGGTGACACACT+TGG 0.539527 4_4:-74735542 Msa0702890:intron
TCTGGGAAGAGGCAGTGCAT+TGG 0.544752 4_4:-74735681 Msa0702890:CDS
AAATTAAATAAGTGGATAGG+TGG 0.544930 4_4:-74734888 Msa0702890:three_prime_UTR
GGATCAGATAAGGCTTGCTA+GGG 0.550417 4_4:-74735137 Msa0702890:CDS
CTTAAATTAAATAAGTGGAT+AGG 0.562680 4_4:-74734891 Msa0702890:three_prime_UTR
TTGGTTCGCTACATAACATC+CGG 0.565357 4_4:+74734780 None:intergenic
AATTAAATAAGTGGATAGGT+GGG 0.568337 4_4:-74734887 Msa0702890:three_prime_UTR
GGTCAGGAAAGGATCAGATA+AGG 0.577849 4_4:-74735147 Msa0702890:CDS
TAGTAGAGAAACATGGCTCT+GGG 0.578678 4_4:-74735698 Msa0702890:exon
TGAGGAGTGAGTGAAGAGGC+AGG 0.584806 4_4:+74735797 None:intergenic
TAAAGAACAGGTACAACAAG+AGG 0.594279 4_4:+74734951 None:intergenic
AAAAGTGTATAGGTCAGGAA+AGG 0.608610 4_4:-74735158 Msa0702890:CDS
TACACTGTTGGAGGTCCTGG+AGG 0.611395 4_4:-74735605 Msa0702890:CDS
CAAATACTAGTAGAGAAACA+TGG 0.613492 4_4:-74735705 Msa0702890:exon
GTGGTGGCAGTGAACAAAGG+AGG 0.631663 4_4:-74735211 Msa0702890:CDS
GATAGTTGCAAGACACCAAG+AGG 0.632266 4_4:-74735184 Msa0702890:CDS
AATGTGGTGGCAGTGAACAA+AGG 0.638717 4_4:-74735214 Msa0702890:CDS
TCCATCAGCTCACAATGTGG+TGG 0.645459 4_4:-74735227 Msa0702890:CDS
TGGTGGCAGTGAACAAAGGA+GGG 0.647438 4_4:-74735210 Msa0702890:CDS
TAATTGAGGAGTGAGTGAAG+AGG 0.656376 4_4:+74735793 None:intergenic
CTGCAAGAAATCTTAATGGG+TGG 0.656921 4_4:+74735821 None:intergenic
ACTCATCTGACCAAGACACG+TGG 0.661487 4_4:+74735882 None:intergenic
TTAAGCCAAAATAATAACAG+TGG 0.665899 4_4:+74734722 None:intergenic
TCCAGGACCTCCAACAGTGT+AGG 0.688043 4_4:+74735607 None:intergenic
GATCAGATAAGGCTTGCTAG+GGG 0.699536 4_4:-74735136 Msa0702890:CDS
TAGTCCATCAGCTCACAATG+TGG 0.719459 4_4:-74735230 Msa0702890:CDS
CTCATCTGACCAAGACACGT+GGG 0.723003 4_4:+74735883 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATAAACATAATGAGTT+TGG - chr4_4:74734733-74734752 Msa0702890:three_prime_UTR 15.0%
!! AATATAAACATAATGAGTTT+GGG - chr4_4:74734734-74734753 Msa0702890:three_prime_UTR 15.0%
!! TTATCCTTAAATTAAATAAG+TGG - chr4_4:74735748-74735767 Msa0702890:five_prime_UTR 15.0%
!! AAATATAAACATAATGAGTT+TGG - chr4_4:74734733-74734752 Msa0702890:three_prime_UTR 15.0%
!! AATATAAACATAATGAGTTT+GGG - chr4_4:74734734-74734753 Msa0702890:three_prime_UTR 15.0%
!! TTATCCTTAAATTAAATAAG+TGG - chr4_4:74735748-74735767 Msa0702890:five_prime_UTR 15.0%
!! TAATAACATTCTTTAACGAT+TGG + chr4_4:74735145-74735164 None:intergenic 20.0%
!! CATTCAATTAATGATGTATA+TGG - chr4_4:74735349-74735368 Msa0702890:intron 20.0%
!!! CTATCCACTTATTTAATTTA+AGG + chr4_4:74735755-74735774 None:intergenic 20.0%
!! CTTAAATTAAATAAGTGGAT+AGG - chr4_4:74735753-74735772 Msa0702890:five_prime_UTR 20.0%
!!! ATTTTGTGTAATATGATTTC+TGG - chr4_4:74735835-74735854 Msa0702890:five_prime_UTR 20.0%
!!! TTTTGTGTAATATGATTTCT+GGG - chr4_4:74735836-74735855 Msa0702890:five_prime_UTR 20.0%
!!! ATTTACCACTGTTATTATTT+TGG - chr4_4:74735917-74735936 Msa0702890:five_prime_UTR 20.0%
!! TAATAACATTCTTTAACGAT+TGG + chr4_4:74735145-74735164 None:intergenic 20.0%
!! CATTCAATTAATGATGTATA+TGG - chr4_4:74735349-74735368 Msa0702890:intron 20.0%
!!! CTATCCACTTATTTAATTTA+AGG + chr4_4:74735755-74735774 None:intergenic 20.0%
!! CTTAAATTAAATAAGTGGAT+AGG - chr4_4:74735753-74735772 Msa0702890:five_prime_UTR 20.0%
!!! ATTTTGTGTAATATGATTTC+TGG - chr4_4:74735835-74735854 Msa0702890:five_prime_UTR 20.0%
!!! TTTTGTGTAATATGATTTCT+GGG - chr4_4:74735836-74735855 Msa0702890:five_prime_UTR 20.0%
!!! ATTTACCACTGTTATTATTT+TGG - chr4_4:74735917-74735936 Msa0702890:five_prime_UTR 20.0%
! TAAATACTTCTTCATATTGC+AGG + chr4_4:74734791-74734810 None:intergenic 25.0%
!!! GTGATAAGGTTTTATAATTG+AGG + chr4_4:74734868-74734887 None:intergenic 25.0%
! AAAAACAACAGTGTTAAAGT+GGG + chr4_4:74735242-74735261 None:intergenic 25.0%
! AAAAAACAACAGTGTTAAAG+TGG + chr4_4:74735243-74735262 None:intergenic 25.0%
! AAGTTGAACACTACATTATT+AGG + chr4_4:74735630-74735649 None:intergenic 25.0%
! AAATTAAATAAGTGGATAGG+TGG - chr4_4:74735756-74735775 Msa0702890:five_prime_UTR 25.0%
! AATTAAATAAGTGGATAGGT+GGG - chr4_4:74735757-74735776 Msa0702890:five_prime_UTR 25.0%
! TTAAGCCAAAATAATAACAG+TGG + chr4_4:74735925-74735944 None:intergenic 25.0%
! TAAATACTTCTTCATATTGC+AGG + chr4_4:74734791-74734810 None:intergenic 25.0%
!!! GTGATAAGGTTTTATAATTG+AGG + chr4_4:74734868-74734887 None:intergenic 25.0%
! AAAAACAACAGTGTTAAAGT+GGG + chr4_4:74735242-74735261 None:intergenic 25.0%
! AAAAAACAACAGTGTTAAAG+TGG + chr4_4:74735243-74735262 None:intergenic 25.0%
! AAGTTGAACACTACATTATT+AGG + chr4_4:74735630-74735649 None:intergenic 25.0%
! AAATTAAATAAGTGGATAGG+TGG - chr4_4:74735756-74735775 Msa0702890:five_prime_UTR 25.0%
! AATTAAATAAGTGGATAGGT+GGG - chr4_4:74735757-74735776 Msa0702890:five_prime_UTR 25.0%
! TTAAGCCAAAATAATAACAG+TGG + chr4_4:74735925-74735944 None:intergenic 25.0%
! GAATTTGGAATGATGTGATA+AGG + chr4_4:74734882-74734901 None:intergenic 30.0%
GATTTGAAGGTAAGTGAATT+TGG + chr4_4:74734897-74734916 None:intergenic 30.0%
CTAGAAAACTGTGAAAGAAA+GGG + chr4_4:74734919-74734938 None:intergenic 30.0%
CAAATACTAGTAGAGAAACA+TGG - chr4_4:74734939-74734958 Msa0702890:three_prime_UTR 30.0%
TCTTCAAAACTCTCTTTCAT+CGG - chr4_4:74735269-74735288 Msa0702890:intron 30.0%
TACTTCATTTGCAACTTTGT+GGG - chr4_4:74735538-74735557 Msa0702890:intron 30.0%
! TAAAAGTTAAGCAGCATTGA+TGG + chr4_4:74735590-74735609 None:intergenic 30.0%
CTACTATATGTTAGTCATCA+TGG - chr4_4:74735798-74735817 Msa0702890:five_prime_UTR 30.0%
! GAATTTGGAATGATGTGATA+AGG + chr4_4:74734882-74734901 None:intergenic 30.0%
GATTTGAAGGTAAGTGAATT+TGG + chr4_4:74734897-74734916 None:intergenic 30.0%
CTAGAAAACTGTGAAAGAAA+GGG + chr4_4:74734919-74734938 None:intergenic 30.0%
CAAATACTAGTAGAGAAACA+TGG - chr4_4:74734939-74734958 Msa0702890:three_prime_UTR 30.0%
TCTTCAAAACTCTCTTTCAT+CGG - chr4_4:74735269-74735288 Msa0702890:intron 30.0%
TACTTCATTTGCAACTTTGT+GGG - chr4_4:74735538-74735557 Msa0702890:intron 30.0%
! TAAAAGTTAAGCAGCATTGA+TGG + chr4_4:74735590-74735609 None:intergenic 30.0%
CTACTATATGTTAGTCATCA+TGG - chr4_4:74735798-74735817 Msa0702890:five_prime_UTR 30.0%
! AAGAAATCTTAATGGGTGGT+TGG + chr4_4:74734822-74734841 None:intergenic 35.0%
TGTGAAAGAAAGGGATTTGA+AGG + chr4_4:74734910-74734929 None:intergenic 35.0%
GCTAGAAAACTGTGAAAGAA+AGG + chr4_4:74734920-74734939 None:intergenic 35.0%
ACTCCAAACATTACTTCATC+TGG - chr4_4:74735189-74735208 Msa0702890:CDS 35.0%
AGACCAGATGAAGTAATGTT+TGG + chr4_4:74735195-74735214 None:intergenic 35.0%
! AAAAGTGTATAGGTCAGGAA+AGG - chr4_4:74735486-74735505 Msa0702890:intron 35.0%
CTACTTCATTTGCAACTTTG+TGG - chr4_4:74735537-74735556 Msa0702890:intron 35.0%
TAAAGAACAGGTACAACAAG+AGG + chr4_4:74735696-74735715 None:intergenic 35.0%
AGTACACATGCATAAAGAAC+AGG + chr4_4:74735708-74735727 None:intergenic 35.0%
!!! TAGCGAACCAATTGTTTTGA+AGG - chr4_4:74735876-74735895 Msa0702890:five_prime_UTR 35.0%
!!! AGCGAACCAATTGTTTTGAA+GGG - chr4_4:74735877-74735896 Msa0702890:five_prime_UTR 35.0%
GTAGTTCCCTTCAAAACAAT+TGG + chr4_4:74735886-74735905 None:intergenic 35.0%
! AAGAAATCTTAATGGGTGGT+TGG + chr4_4:74734822-74734841 None:intergenic 35.0%
TGTGAAAGAAAGGGATTTGA+AGG + chr4_4:74734910-74734929 None:intergenic 35.0%
GCTAGAAAACTGTGAAAGAA+AGG + chr4_4:74734920-74734939 None:intergenic 35.0%
ACTCCAAACATTACTTCATC+TGG - chr4_4:74735189-74735208 Msa0702890:CDS 35.0%
AGACCAGATGAAGTAATGTT+TGG + chr4_4:74735195-74735214 None:intergenic 35.0%
! AAAAGTGTATAGGTCAGGAA+AGG - chr4_4:74735486-74735505 Msa0702890:intron 35.0%
CTACTTCATTTGCAACTTTG+TGG - chr4_4:74735537-74735556 Msa0702890:intron 35.0%
TAAAGAACAGGTACAACAAG+AGG + chr4_4:74735696-74735715 None:intergenic 35.0%
AGTACACATGCATAAAGAAC+AGG + chr4_4:74735708-74735727 None:intergenic 35.0%
!!! TAGCGAACCAATTGTTTTGA+AGG - chr4_4:74735876-74735895 Msa0702890:five_prime_UTR 35.0%
!!! AGCGAACCAATTGTTTTGAA+GGG - chr4_4:74735877-74735896 Msa0702890:five_prime_UTR 35.0%
GTAGTTCCCTTCAAAACAAT+TGG + chr4_4:74735886-74735905 None:intergenic 35.0%
CTGCAAGAAATCTTAATGGG+TGG + chr4_4:74734826-74734845 None:intergenic 40.0%
CACCCATTAAGATTTCTTGC+AGG - chr4_4:74734824-74734843 Msa0702890:three_prime_UTR 40.0%
GGCCTGCAAGAAATCTTAAT+GGG + chr4_4:74734829-74734848 None:intergenic 40.0%
AGGCCTGCAAGAAATCTTAA+TGG + chr4_4:74734830-74734849 None:intergenic 40.0%
TAATTGAGGAGTGAGTGAAG+AGG + chr4_4:74734854-74734873 None:intergenic 40.0%
TAGTAGAGAAACATGGCTCT+GGG - chr4_4:74734946-74734965 Msa0702890:three_prime_UTR 40.0%
! GCCTAAGGGAAAACGTTTTA+GGG - chr4_4:74735086-74735105 Msa0702890:CDS 40.0%
! CTATACACTTTTGCTCCTCT+TGG + chr4_4:74735478-74735497 None:intergenic 40.0%
! CAAGAGGAGCAAAAGTGTAT+AGG - chr4_4:74735476-74735495 Msa0702890:intron 40.0%
! ATATGATTTCTGGGTCTCTC+CGG - chr4_4:74735845-74735864 Msa0702890:five_prime_UTR 40.0%
! TTGGTTCGCTACATAACATC+CGG + chr4_4:74735867-74735886 None:intergenic 40.0%
CTGCAAGAAATCTTAATGGG+TGG + chr4_4:74734826-74734845 None:intergenic 40.0%
CACCCATTAAGATTTCTTGC+AGG - chr4_4:74734824-74734843 Msa0702890:three_prime_UTR 40.0%
GGCCTGCAAGAAATCTTAAT+GGG + chr4_4:74734829-74734848 None:intergenic 40.0%
AGGCCTGCAAGAAATCTTAA+TGG + chr4_4:74734830-74734849 None:intergenic 40.0%
TAATTGAGGAGTGAGTGAAG+AGG + chr4_4:74734854-74734873 None:intergenic 40.0%
TAGTAGAGAAACATGGCTCT+GGG - chr4_4:74734946-74734965 Msa0702890:three_prime_UTR 40.0%
! GCCTAAGGGAAAACGTTTTA+GGG - chr4_4:74735086-74735105 Msa0702890:CDS 40.0%
! CTATACACTTTTGCTCCTCT+TGG + chr4_4:74735478-74735497 None:intergenic 40.0%
! CAAGAGGAGCAAAAGTGTAT+AGG - chr4_4:74735476-74735495 Msa0702890:intron 40.0%
! ATATGATTTCTGGGTCTCTC+CGG - chr4_4:74735845-74735864 Msa0702890:five_prime_UTR 40.0%
! TTGGTTCGCTACATAACATC+CGG + chr4_4:74735867-74735886 None:intergenic 40.0%
CTAGTAGAGAAACATGGCTC+TGG - chr4_4:74734945-74734964 Msa0702890:three_prime_UTR 45.0%
GGTTGGACCTTTAACACTGT+TGG - chr4_4:74735060-74735079 Msa0702890:exon 45.0%
GGACCTTTAACACTGTTGGT+TGG - chr4_4:74735064-74735083 Msa0702890:CDS 45.0%
AGGCCAACCAACAGTGTTAA+AGG + chr4_4:74735070-74735089 None:intergenic 45.0%
!! TAACACTGTTGGTTGGCCTA+AGG - chr4_4:74735071-74735090 Msa0702890:CDS 45.0%
!! AACACTGTTGGTTGGCCTAA+GGG - chr4_4:74735072-74735091 Msa0702890:CDS 45.0%
! GGCCTAAGGGAAAACGTTTT+AGG - chr4_4:74735085-74735104 Msa0702890:CDS 45.0%
! GCCCTAAAACGTTTTCCCTT+AGG + chr4_4:74735090-74735109 None:intergenic 45.0%
!!! AAGGGAAAACGTTTTAGGGC+TGG - chr4_4:74735090-74735109 Msa0702890:CDS 45.0%
TAGTCCATCAGCTCACAATG+TGG - chr4_4:74735414-74735433 Msa0702890:intron 45.0%
AATGTGGTGGCAGTGAACAA+AGG - chr4_4:74735430-74735449 Msa0702890:intron 45.0%
GATAGTTGCAAGACACCAAG+AGG - chr4_4:74735460-74735479 Msa0702890:intron 45.0%
! GGAGCAAAAGTGTATAGGTC+AGG - chr4_4:74735481-74735500 Msa0702890:intron 45.0%
GGTCAGGAAAGGATCAGATA+AGG - chr4_4:74735497-74735516 Msa0702890:intron 45.0%
! AGGATCAGATAAGGCTTGCT+AGG - chr4_4:74735506-74735525 Msa0702890:intron 45.0%
!! GGATCAGATAAGGCTTGCTA+GGG - chr4_4:74735507-74735526 Msa0702890:intron 45.0%
!! GATCAGATAAGGCTTGCTAG+GGG - chr4_4:74735508-74735527 Msa0702890:intron 45.0%
CTAGTAGAGAAACATGGCTC+TGG - chr4_4:74734945-74734964 Msa0702890:three_prime_UTR 45.0%
GGTTGGACCTTTAACACTGT+TGG - chr4_4:74735060-74735079 Msa0702890:exon 45.0%
GGACCTTTAACACTGTTGGT+TGG - chr4_4:74735064-74735083 Msa0702890:CDS 45.0%
AGGCCAACCAACAGTGTTAA+AGG + chr4_4:74735070-74735089 None:intergenic 45.0%
!! TAACACTGTTGGTTGGCCTA+AGG - chr4_4:74735071-74735090 Msa0702890:CDS 45.0%
!! AACACTGTTGGTTGGCCTAA+GGG - chr4_4:74735072-74735091 Msa0702890:CDS 45.0%
! GGCCTAAGGGAAAACGTTTT+AGG - chr4_4:74735085-74735104 Msa0702890:CDS 45.0%
! GCCCTAAAACGTTTTCCCTT+AGG + chr4_4:74735090-74735109 None:intergenic 45.0%
!!! AAGGGAAAACGTTTTAGGGC+TGG - chr4_4:74735090-74735109 Msa0702890:CDS 45.0%
TAGTCCATCAGCTCACAATG+TGG - chr4_4:74735414-74735433 Msa0702890:intron 45.0%
AATGTGGTGGCAGTGAACAA+AGG - chr4_4:74735430-74735449 Msa0702890:intron 45.0%
GATAGTTGCAAGACACCAAG+AGG - chr4_4:74735460-74735479 Msa0702890:intron 45.0%
! GGAGCAAAAGTGTATAGGTC+AGG - chr4_4:74735481-74735500 Msa0702890:intron 45.0%
GGTCAGGAAAGGATCAGATA+AGG - chr4_4:74735497-74735516 Msa0702890:intron 45.0%
! AGGATCAGATAAGGCTTGCT+AGG - chr4_4:74735506-74735525 Msa0702890:intron 45.0%
!! GGATCAGATAAGGCTTGCTA+GGG - chr4_4:74735507-74735526 Msa0702890:intron 45.0%
!! GATCAGATAAGGCTTGCTAG+GGG - chr4_4:74735508-74735527 Msa0702890:intron 45.0%
CTCATCTGACCAAGACACGT+GGG + chr4_4:74734764-74734783 None:intergenic 50.0%
ACTCATCTGACCAAGACACG+TGG + chr4_4:74734765-74734784 None:intergenic 50.0%
!! AGAAACATGGCTCTGGGAAG+AGG - chr4_4:74734952-74734971 Msa0702890:three_prime_UTR 50.0%
CATGCTGCTACCTACACTGT+TGG - chr4_4:74735027-74735046 Msa0702890:three_prime_UTR 50.0%
GTGTTAAAGGTCCAACCTCC+AGG + chr4_4:74735057-74735076 None:intergenic 50.0%
! TTTAGGGCTGGTGACACACT+TGG - chr4_4:74735102-74735121 Msa0702890:CDS 50.0%
TCCATCAGCTCACAATGTGG+TGG - chr4_4:74735417-74735436 Msa0702890:intron 50.0%
TGGTGGCAGTGAACAAAGGA+GGG - chr4_4:74735434-74735453 Msa0702890:intron 50.0%
CTCATCTGACCAAGACACGT+GGG + chr4_4:74734764-74734783 None:intergenic 50.0%
ACTCATCTGACCAAGACACG+TGG + chr4_4:74734765-74734784 None:intergenic 50.0%
!! AGAAACATGGCTCTGGGAAG+AGG - chr4_4:74734952-74734971 Msa0702890:three_prime_UTR 50.0%
CATGCTGCTACCTACACTGT+TGG - chr4_4:74735027-74735046 Msa0702890:three_prime_UTR 50.0%
GTGTTAAAGGTCCAACCTCC+AGG + chr4_4:74735057-74735076 None:intergenic 50.0%
! TTTAGGGCTGGTGACACACT+TGG - chr4_4:74735102-74735121 Msa0702890:CDS 50.0%
TCCATCAGCTCACAATGTGG+TGG - chr4_4:74735417-74735436 Msa0702890:intron 50.0%
TGGTGGCAGTGAACAAAGGA+GGG - chr4_4:74735434-74735453 Msa0702890:intron 50.0%
TGAGGAGTGAGTGAAGAGGC+AGG + chr4_4:74734850-74734869 None:intergenic 55.0%
TCTGGGAAGAGGCAGTGCAT+TGG - chr4_4:74734963-74734982 Msa0702890:three_prime_UTR 55.0%
GCTGCTACCTACACTGTTGG+AGG - chr4_4:74735030-74735049 Msa0702890:three_prime_UTR 55.0%
ACCTACACTGTTGGAGGTCC+TGG - chr4_4:74735036-74735055 Msa0702890:three_prime_UTR 55.0%
TCCAGGACCTCCAACAGTGT+AGG + chr4_4:74735040-74735059 None:intergenic 55.0%
TACACTGTTGGAGGTCCTGG+AGG - chr4_4:74735039-74735058 Msa0702890:three_prime_UTR 55.0%
GCCACCACATTGTGAGCTGA+TGG + chr4_4:74735421-74735440 None:intergenic 55.0%
GTGGTGGCAGTGAACAAAGG+AGG - chr4_4:74735433-74735452 Msa0702890:intron 55.0%
TTTGTGGGTCACTGCGAGTC+TGG - chr4_4:74735553-74735572 Msa0702890:CDS 55.0%
TGAGGAGTGAGTGAAGAGGC+AGG + chr4_4:74734850-74734869 None:intergenic 55.0%
TCTGGGAAGAGGCAGTGCAT+TGG - chr4_4:74734963-74734982 Msa0702890:three_prime_UTR 55.0%
GCTGCTACCTACACTGTTGG+AGG - chr4_4:74735030-74735049 Msa0702890:three_prime_UTR 55.0%
ACCTACACTGTTGGAGGTCC+TGG - chr4_4:74735036-74735055 Msa0702890:three_prime_UTR 55.0%
TCCAGGACCTCCAACAGTGT+AGG + chr4_4:74735040-74735059 None:intergenic 55.0%
TACACTGTTGGAGGTCCTGG+AGG - chr4_4:74735039-74735058 Msa0702890:three_prime_UTR 55.0%
GCCACCACATTGTGAGCTGA+TGG + chr4_4:74735421-74735440 None:intergenic 55.0%
GTGGTGGCAGTGAACAAAGG+AGG - chr4_4:74735433-74735452 Msa0702890:intron 55.0%
TTTGTGGGTCACTGCGAGTC+TGG - chr4_4:74735553-74735572 Msa0702890:CDS 55.0%
TTGGGCAGACCCACGTGTCT+TGG - chr4_4:74734752-74734771 Msa0702890:three_prime_UTR 60.0%
CTGTTGGAGGTCCTGGAGGT+TGG - chr4_4:74735043-74735062 Msa0702890:three_prime_UTR 60.0%
TTGGGCAGACCCACGTGTCT+TGG - chr4_4:74734752-74734771 Msa0702890:three_prime_UTR 60.0%
CTGTTGGAGGTCCTGGAGGT+TGG - chr4_4:74735043-74735062 Msa0702890:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 74734721 74735945 74734721 ID=Msa0702890;Name=Msa0702890
chr4_4 mRNA 74734721 74735945 74734721 ID=Msa0702890-mRNA-1;Parent=Msa0702890;Name=Msa0702890-mRNA-1;_AED=0.00;_eAED=0.00;_QI=237|1|1|1|1|1|2|342|121
chr4_4 exon 74734721 74735262 74734721 ID=Msa0702890-mRNA-1:exon:23323;Parent=Msa0702890-mRNA-1
chr4_4 exon 74735543 74735945 74735543 ID=Msa0702890-mRNA-1:exon:23322;Parent=Msa0702890-mRNA-1
chr4_4 five_prime_UTR 74735709 74735945 74735709 ID=Msa0702890-mRNA-1:five_prime_utr;Parent=Msa0702890-mRNA-1
chr4_4 CDS 74735543 74735708 74735543 ID=Msa0702890-mRNA-1:cds;Parent=Msa0702890-mRNA-1
chr4_4 CDS 74735063 74735262 74735063 ID=Msa0702890-mRNA-1:cds;Parent=Msa0702890-mRNA-1
chr4_4 three_prime_UTR 74734721 74735062 74734721 ID=Msa0702890-mRNA-1:three_prime_utr;Parent=Msa0702890-mRNA-1
Gene Sequence

>Msa0702890

ATGGCTCTGGGAAGAGGCAGTGCATTGGTTCTACTAGTCTGCTTTTTTGTTCTTAACTCTGAGTTTGCTCATGCTGCTACCTACACTGTTGGAGGTCCTGGAGGTTGGACCTTTAACACTGTTGGTTGGCCTAAGGGAAAACGTTTTAGGGCTGGTGACACACTTGTGTTCAACTATAGTCCATCAGCTCACAATGTGGTGGCAGTGAACAAAGGAGGGTATGATAGTTGCAAGACACCAAGAGGAGCAAAAGTGTATAGGTCAGGAAAGGATCAGATAAGGCTTGCTAGGGGACAAAACTACTTCATTTGCAACTTTGTGGGTCACTGCGAGTCTGGAATGAAAATTGCCATCAATGCTGCTTAA
Protein sequence

>Msa0702890

MALGRGSALVLLVCFFVLNSEFAHAATYTVGGPGGWTFNTVGWPKGKRFRAGDTLVFNYSPSAHNVVAVNKGGYDSCKTPRGAKVYRSGKDQIRLARGQNYFICNFVGHCESGMKIAINAA*