AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0612520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0612520 MtrunA17_Chr4g0047171 90.741 216 10 1 75 290 71 276 8.15e-98 288
Msa0612520 MtrunA17_Chr4g0024211 56.055 289 87 9 1 288 1 250 3.54e-75 230
Msa0612520 MtrunA17_Chr1g0176911 48.276 348 113 9 1 288 1 341 9.66e-74 229
Msa0612520 MtrunA17_Chr5g0420431 48.485 363 89 12 1 288 1 340 3.22e-73 228
Msa0612520 MtrunA17_Chr4g0024201 45.806 310 81 10 16 288 16 275 2.25e-63 201
Msa0612520 MtrunA17_Chr3g0081151 44.279 402 99 12 1 288 1 391 8.41e-62 200
Msa0612520 MtrunA17_Chr4g0033381 56.989 186 63 3 105 290 170 338 6.37e-60 194
Msa0612520 MtrunA17_Chr6g0466861 48.029 279 93 7 15 288 16 247 1.95e-47 159
Msa0612520 MtrunA17_Chr4g0007081 63.636 143 48 1 147 289 208 346 1.12e-46 160
Msa0612520 MtrunA17_Chr4g0047181 36.743 479 104 13 1 288 45 515 1.56e-46 163
Msa0612520 MtrunA17_Chr8g0383411 58.929 112 41 2 179 289 269 376 5.94e-37 135
Msa0612520 MtrunA17_Chr2g0328311 62.195 82 30 1 207 288 2 82 4.34e-27 101
Msa0612520 MtrunA17_Chr4g0069771 42.515 167 55 3 40 193 68 206 3.31e-21 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0612520 AT2G34700.1 44.966 149 75 2 146 288 26 173 2.04e-37 131
Msa0612520 AT2G33790.1 37.681 207 118 5 93 290 35 239 1.41e-29 112
Msa0612520 AT1G28290.2 45.926 135 64 3 163 290 183 315 3.07e-29 113
Msa0612520 AT1G28290.1 45.926 135 64 3 163 290 227 359 4.68e-29 114

Find 138 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCTTCCTCTCTCTTTCT+AGG 0.176186 4_2:-60975521 Msa0612520:five_prime_UTR
TCTCTTACACTTTGAGAAAA+TGG 0.191278 4_2:-60975491 Msa0612520:exon
GTGAGTGAGGGTTGATTTAT+AGG 0.202696 4_2:+60975569 None:intergenic
TGCTGGTGTTGATACTCTTT+TGG 0.212871 4_2:-60974936 Msa0612520:CDS
TTGTGTGAGGAGGGTAAGTT+GGG 0.250591 4_2:+60975389 None:intergenic
TCCACTTTCGTGTTAAATTA+TGG 0.251191 4_2:-60973062 Msa0612520:three_prime_UTR
GCATAGCTTGTGATGTTCTT+TGG 0.271925 4_2:+60973329 None:intergenic
AACCTTCATGGTGGTCTTAC+CGG 0.294151 4_2:-60973251 Msa0612520:CDS
TGAACAGCTATAAAGCTTCT+AGG 0.305603 4_2:+60974989 None:intergenic
TTGGGAGCTACTCAAATTCT+TGG 0.313293 4_2:-60974917 Msa0612520:intron
TTGGAACAGGAGGGTGTAAT+GGG 0.327634 4_2:+60975071 None:intergenic
GTTGTGTGAGGAGGGTAAGT+TGG 0.333157 4_2:+60975388 None:intergenic
AACCGTTCTTATCAGTGGTC+TGG 0.337205 4_2:+60973369 None:intergenic
GGGTGTAGAGGGGTGTGAAT+AGG 0.348091 4_2:+60975253 None:intergenic
TCGTGTTAAATTATGGTGTT+TGG 0.355340 4_2:-60973055 Msa0612520:three_prime_UTR
AGGGGTGTAGGATGCAATGG+TGG 0.362731 4_2:+60975013 None:intergenic
CTTAGCCACAAACGGCTTCT+CGG 0.373734 4_2:+60973214 None:intergenic
CGAGAAGCCGTTTGTGGCTA+AGG 0.379375 4_2:-60973213 Msa0612520:CDS
GGTGGGTGTGCTGGAGCAGG+TGG 0.379735 4_2:+60975031 None:intergenic
GGGTGTAGTGGTGGATGTGC+TGG 0.382371 4_2:+60975127 None:intergenic
GGTGGTGGTGATGGTGGTGA+GGG 0.385537 4_2:+60975311 None:intergenic
TCCAACCGTGTAGAGAATAA+AGG 0.386366 4_2:+60973184 None:intergenic
GGAGCAACAGGTGTGTGGAC+TGG 0.395530 4_2:+60975148 None:intergenic
TTAAAGCCTTCGAACCTTCA+TGG 0.396084 4_2:-60973263 Msa0612520:CDS
GAGCAACAGGTGTGTGGACT+GGG 0.397889 4_2:+60975149 None:intergenic
CCCTTTATTCTCTACACGGT+TGG 0.398136 4_2:-60973185 Msa0612520:CDS
AGTGAATGTGGTGGTGGTGA+TGG 0.399069 4_2:+60975302 None:intergenic
TGGTGGTGGTGATGGTGGTG+AGG 0.400493 4_2:+60975310 None:intergenic
GTTGGTGGGTGAAGTGGAGC+TGG 0.401765 4_2:+60975364 None:intergenic
CAGGGGTTGGTGAGTGAATG+TGG 0.410018 4_2:+60975290 None:intergenic
GTCTTACCGGTGCTGGTCTT+AGG 0.411218 4_2:-60973238 Msa0612520:CDS
CCTCTTCCTCTCTCTTTCTA+GGG 0.411759 4_2:-60975520 Msa0612520:five_prime_UTR
TAGTTGGGTATACGGGTTAC+GGG 0.414287 4_2:-60972872 None:intergenic
GCGTTAGCCGGTGGGTGAAC+CGG 0.414913 4_2:+60975334 None:intergenic
TTAGTTGGGTATACGGGTTA+CGG 0.415140 4_2:-60972873 None:intergenic
GAGCAGGAGGGTGAGGATGA+TGG 0.417914 4_2:+60975176 None:intergenic
ACCGTTCTTATCAGTGGTCT+GGG 0.419238 4_2:+60973370 None:intergenic
GGTGAGGATGATGGTGGTGG+TGG 0.424562 4_2:+60975185 None:intergenic
TTCGAAGGCTTTAATCCATT+CGG 0.425021 4_2:+60973272 None:intergenic
GTTGGAACAGGAGGGTGTAA+TGG 0.425934 4_2:+60975070 None:intergenic
CATGGTGGTCTTACCGGTGC+TGG 0.430807 4_2:-60973245 Msa0612520:CDS
TGGTGGTGAGGGGCGTTAGC+CGG 0.434825 4_2:+60975322 None:intergenic
CCGTGAGTTGGTGGTTTAGC+AGG 0.434974 4_2:+60975208 None:intergenic
GTGTGGACTGGGGAAGGAGC+AGG 0.436294 4_2:+60975160 None:intergenic
TTGGTGAGTGAATGTGGTGG+TGG 0.439920 4_2:+60975296 None:intergenic
TTAGCCACAAACGGCTTCTC+GGG 0.442517 4_2:+60973215 None:intergenic
AATGGTGGGTGTGCTGGAGC+AGG 0.443203 4_2:+60975028 None:intergenic
GAGGGTGAGGATGATGGTGG+TGG 0.446074 4_2:+60975182 None:intergenic
GGATGCAATGGTGGGTGTGC+TGG 0.446386 4_2:+60975022 None:intergenic
TAGGAGAAGGAGTAGGAGCA+GGG 0.447008 4_2:+60975272 None:intergenic
ATAGGAGAAGGAGTAGGAGC+AGG 0.447520 4_2:+60975271 None:intergenic
AGAAGCTTTATAGCTGTTCA+AGG 0.449540 4_2:-60974986 Msa0612520:CDS
GCTGGAGCAGGTGGGTGTAA+TGG 0.449877 4_2:+60975040 None:intergenic
GGGGAAGGAGCAGGAGGGTG+AGG 0.451502 4_2:+60975169 None:intergenic
GTGTGAACAGGTGGGTGTAG+TGG 0.456043 4_2:+60975115 None:intergenic
GGTGGATGTGCTGGAGCAAC+AGG 0.458900 4_2:+60975136 None:intergenic
CCCTAGAAAGAGAGAGGAAG+AGG 0.459003 4_2:+60975520 None:intergenic
TGAACAGGTGGGTGTAGTGG+TGG 0.461198 4_2:+60975118 None:intergenic
AATGGAGGGTGAGTTGGAAC+AGG 0.461672 4_2:+60975058 None:intergenic
GGAGCAGGTGGGTGTAATGG+AGG 0.467004 4_2:+60975043 None:intergenic
GGGTGTAATGGAGGGTGAGT+TGG 0.472310 4_2:+60975052 None:intergenic
CCAACCGTGTAGAGAATAAA+GGG 0.474781 4_2:+60973185 None:intergenic
ACCATAATTTAACACGAAAG+TGG 0.478975 4_2:+60973061 None:intergenic
AATGGTAAGGAGGGTGTAGA+GGG 0.479744 4_2:+60975242 None:intergenic
AGAGGAAGAGGATGTGATGT+TGG 0.480795 4_2:+60975532 None:intergenic
GGAACAACAGGGGTGTGAAC+AGG 0.484015 4_2:+60975103 None:intergenic
TAGGCCCGAGAAGCCGTTTG+TGG 0.488242 4_2:-60973219 Msa0612520:CDS
GTTGGTGGTTTAGCAGGTAC+AGG 0.492827 4_2:+60975214 None:intergenic
TGGTGGTGGCCGTGAGTTGG+TGG 0.495728 4_2:+60975199 None:intergenic
GGGGTGTAGGATGCAATGGT+GGG 0.495833 4_2:+60975014 None:intergenic
TGAACCGGTGACTTGGTTGG+TGG 0.499429 4_2:+60975349 None:intergenic
GGTGAGGGGCGTTAGCCGGT+GGG 0.507227 4_2:+60975326 None:intergenic
GAATGTGGTGGTGGTGATGG+TGG 0.512130 4_2:+60975305 None:intergenic
GAAGGAGTAGGAGCAGGGGT+TGG 0.512859 4_2:+60975277 None:intergenic
TGGACTGGGGAAGGAGCAGG+AGG 0.517336 4_2:+60975163 None:intergenic
GAATGGTAAGGAGGGTGTAG+AGG 0.518563 4_2:+60975241 None:intergenic
GAACAGCTATAAAGCTTCTA+GGG 0.519875 4_2:+60974990 None:intergenic
GTAAAAGTAGCAATATTGCA+AGG 0.527067 4_2:+60975461 None:intergenic
GACTTGGTTGGTGGGTGAAG+TGG 0.529058 4_2:+60975358 None:intergenic
GAACCGGTGACTTGGTTGGT+GGG 0.532249 4_2:+60975350 None:intergenic
TGGTGGTGGTGGCCGTGAGT+TGG 0.535050 4_2:+60975196 None:intergenic
GGAAGACCCTTAGCCACAAA+CGG 0.535213 4_2:+60973206 None:intergenic
GTGGGTGTGCTGGAGCAGGT+GGG 0.537085 4_2:+60975032 None:intergenic
TGGTGAGGGGCGTTAGCCGG+TGG 0.537298 4_2:+60975325 None:intergenic
AGAGGGGTGTGAATAGGAGA+AGG 0.538140 4_2:+60975259 None:intergenic
ACAACAGGGGTGTGAACAGG+TGG 0.540027 4_2:+60975106 None:intergenic
AAGCTTCTAGGGAGGGGTGT+AGG 0.543464 4_2:+60975001 None:intergenic
GGGTGAACCGGTGACTTGGT+TGG 0.547932 4_2:+60975346 None:intergenic
ACAGGTGTAGAATGGTAAGG+AGG 0.548661 4_2:+60975232 None:intergenic
GTGGTGGTGATGGTGGTGAG+GGG 0.548870 4_2:+60975312 None:intergenic
GAGGGTGAGTTGGAACAGGA+GGG 0.549639 4_2:+60975062 None:intergenic
ACAGGTGTGTGGACTGGGGA+AGG 0.551806 4_2:+60975154 None:intergenic
AGGAGAAGGAGTAGGAGCAG+GGG 0.557814 4_2:+60975273 None:intergenic
GAGCTGGTGTTGTGTGAGGA+GGG 0.558920 4_2:+60975380 None:intergenic
GTCAAATCTTGCAAGTATGC+TGG 0.559072 4_2:-60974953 Msa0612520:CDS
GGAGGGTGAGTTGGAACAGG+AGG 0.559242 4_2:+60975061 None:intergenic
CGGTGGGTGAACCGGTGACT+TGG 0.560182 4_2:+60975342 None:intergenic
GGAACAGGAGGGTGTAATGG+GGG 0.562210 4_2:+60975073 None:intergenic
GCTATAAAGCTTCTAGGGAG+GGG 0.564039 4_2:+60974995 None:intergenic
CAAGTCACCGGTTCACCCAC+CGG 0.566063 4_2:-60975341 Msa0612520:CDS
TGGAACAGGAGGGTGTAATG+GGG 0.566240 4_2:+60975072 None:intergenic
CTCGGGCCTAAGACCAGCAC+CGG 0.566604 4_2:+60973232 None:intergenic
GAGAAGCCGTTTGTGGCTAA+GGG 0.568492 4_2:-60973212 Msa0612520:CDS
CAGGAGGGTGAGGATGATGG+TGG 0.571819 4_2:+60975179 None:intergenic
GTTATGAGAGAGTGTGAGTG+AGG 0.572156 4_2:+60975556 None:intergenic
AGCTATAAAGCTTCTAGGGA+GGG 0.575095 4_2:+60974994 None:intergenic
TAGCAGGTACAGGTGTAGAA+TGG 0.575670 4_2:+60975224 None:intergenic
GGGAGGGGTGTAGGATGCAA+TGG 0.575724 4_2:+60975010 None:intergenic
AAAATAACCGTTCTTATCAG+TGG 0.576999 4_2:+60973364 None:intergenic
GGTACAGGTGTAGAATGGTA+AGG 0.578784 4_2:+60975229 None:intergenic
TCACCCACCAACCAAGTCAC+CGG 0.586790 4_2:-60975353 Msa0612520:CDS
CAGGTGTAGAATGGTAAGGA+GGG 0.586995 4_2:+60975233 None:intergenic
GGAGCTGGTGTTGTGTGAGG+AGG 0.590088 4_2:+60975379 None:intergenic
TAAAAGTAGCAATATTGCAA+GGG 0.590364 4_2:+60975462 None:intergenic
AGTGGAGCTGGTGTTGTGTG+AGG 0.593333 4_2:+60975376 None:intergenic
GTGTGAATAGGAGAAGGAGT+AGG 0.597611 4_2:+60975265 None:intergenic
GGGGATGAGTTGGAACAACA+GGG 0.598634 4_2:+60975092 None:intergenic
GAGCAGGTGGGTGTAATGGA+GGG 0.598759 4_2:+60975044 None:intergenic
AAGTAACCCTAGAAAGAGAG+AGG 0.599079 4_2:+60975514 None:intergenic
TCTTCCCTTTATTCTCTACA+CGG 0.602477 4_2:-60973189 Msa0612520:CDS
CACCGGTAAGACCACCATGA+AGG 0.605543 4_2:+60973249 None:intergenic
CAACAACACAAAGTACAAGT+TGG 0.607796 4_2:-60973402 Msa0612520:CDS
ATGGTAAGGAGGGTGTAGAG+GGG 0.618924 4_2:+60975243 None:intergenic
AGACCACCATGAAGGTTCGA+AGG 0.625392 4_2:+60973257 None:intergenic
TTATGAGAGAGTGTGAGTGA+GGG 0.631870 4_2:+60975557 None:intergenic
GTGCTGGAGCAACAGGTGTG+TGG 0.635755 4_2:+60975143 None:intergenic
CAGCTATAAAGCTTCTAGGG+AGG 0.636143 4_2:+60974993 None:intergenic
CAACAGGGGTGTGAACAGGT+GGG 0.648196 4_2:+60975107 None:intergenic
GGGTTGGTGAGTGAATGTGG+TGG 0.650934 4_2:+60975293 None:intergenic
ACCCAGACCACTGATAAGAA+CGG 0.652681 4_2:-60973371 Msa0612520:CDS
CCTGCTAAACCACCAACTCA+CGG 0.657475 4_2:-60975208 Msa0612520:CDS
CATTTAGGCTCAAATGCAAG+AGG 0.660159 4_2:+60973161 None:intergenic
GGACTGGGGAAGGAGCAGGA+GGG 0.674543 4_2:+60975164 None:intergenic
GTTGCATTGAAGCTTCACAA+CGG 0.689892 4_2:+60973421 None:intergenic
AGCAACAGGTGTGTGGACTG+GGG 0.697477 4_2:+60975150 None:intergenic
GGGATGAGTTGGAACAACAG+GGG 0.726853 4_2:+60975093 None:intergenic
AAGCCTTCGAACCTTCATGG+TGG 0.750456 4_2:-60973260 Msa0612520:CDS
TGTGTGAGGAGGGTAAGTTG+GGG 0.787508 4_2:+60975390 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTATGAAAAAATTTTAATTT+TGG + chr4_2:60974423-60974442 None:intergenic 10.0%
!!! AAAAAATAGAGTATATTTTA+AGG - chr4_2:60974683-60974702 Msa0612520:intron 10.0%
!! TATTAGATGAACAAAAAAAT+GGG + chr4_2:60973973-60973992 None:intergenic 15.0%
!! ATATTAGATGAACAAAAAAA+TGG + chr4_2:60973974-60973993 None:intergenic 15.0%
!!! ATGTTGTGAAATTATTTTTT+AGG - chr4_2:60974306-60974325 Msa0612520:intron 15.0%
!!! TAAGTACATTAATTACTTTT+AGG + chr4_2:60974563-60974582 None:intergenic 15.0%
!!! ATAATAGTACATTATTTTGT+TGG - chr4_2:60974924-60974943 Msa0612520:CDS 15.0%
!!! TAATAGTACATTATTTTGTT+GGG - chr4_2:60974925-60974944 Msa0612520:CDS 15.0%
!! TTTCAAAATACATAAAATGA+TGG + chr4_2:60975007-60975026 None:intergenic 15.0%
!!! ATAAACTCTAATTTTCTTAA+CGG + chr4_2:60975330-60975349 None:intergenic 15.0%
!! TTGAATTGAACTATGAATAT+TGG - chr4_2:60973779-60973798 Msa0612520:intron 20.0%
!! TCCAAAGTTTAGAAAAATAT+TGG - chr4_2:60974063-60974082 Msa0612520:intron 20.0%
!!! ACCAATATTTTTCTAAACTT+TGG + chr4_2:60974067-60974086 None:intergenic 20.0%
!!! ATTTTTTTTTTGACAAGTCA+TGG + chr4_2:60974162-60974181 None:intergenic 20.0%
!!! AAAGTGAGTATTTTTCTATT+CGG + chr4_2:60974643-60974662 None:intergenic 20.0%
!! TTAATCATTGTTTGCAATTA+AGG - chr4_2:60974781-60974800 Msa0612520:intron 20.0%
!! TAATCATTGTTTGCAATTAA+GGG - chr4_2:60974782-60974801 Msa0612520:intron 20.0%
!!! TTTTATTTCTTTACCATTCT+TGG + chr4_2:60974852-60974871 None:intergenic 20.0%
!!! GTATTTTGAAATTGAATTGT+AGG - chr4_2:60975016-60975035 Msa0612520:CDS 20.0%
!!! TAAACTCTAATTTTCTTAAC+GGG + chr4_2:60975329-60975348 None:intergenic 20.0%
! TAAAAGTAGCAATATTGCAA+GGG + chr4_2:60973003-60973022 None:intergenic 25.0%
! CTAAAAAGGGAATAATTGTT+AGG + chr4_2:60973899-60973918 None:intergenic 25.0%
!! CTAACAATTATTCCCTTTTT+AGG - chr4_2:60973897-60973916 Msa0612520:intron 25.0%
! ATCACAGATAAACCTAAAAA+GGG + chr4_2:60973912-60973931 None:intergenic 25.0%
! ACTTTGGATTTGTAAACTTT+GGG + chr4_2:60974051-60974070 None:intergenic 25.0%
! AACTTTGGATTTGTAAACTT+TGG + chr4_2:60974052-60974071 None:intergenic 25.0%
!!! TGCTTACATGTGTTTTTTTA+GGG + chr4_2:60974223-60974242 None:intergenic 25.0%
!!! TTGCTTACATGTGTTTTTTT+AGG + chr4_2:60974224-60974243 None:intergenic 25.0%
! AACAATGATTAAAGTTTCTC+CGG + chr4_2:60974773-60974792 None:intergenic 25.0%
!!! AACGGTTATTTTTTCATTGA+AGG - chr4_2:60975109-60975128 Msa0612520:CDS 25.0%
!! AAACTCTAATTTTCTTAACG+GGG + chr4_2:60975328-60975347 None:intergenic 25.0%
!!! TTCTCAGAAACTATTTTAGT+TGG - chr4_2:60975574-60975593 Msa0612520:five_prime_UTR 25.0%
!!! TCTCAGAAACTATTTTAGTT+GGG - chr4_2:60975575-60975594 Msa0612520:five_prime_UTR 25.0%
!!! AACTATTTTAGTTGGGTATA+CGG - chr4_2:60975582-60975601 Msa0612520:five_prime_UTR 25.0%
! TCTCTTACACTTTGAGAAAA+TGG - chr4_2:60972971-60972990 Msa0612520:three_prime_UTR 30.0%
GTAAAAGTAGCAATATTGCA+AGG + chr4_2:60973004-60973023 None:intergenic 30.0%
TCATAGTTCAATTCAAACAG+TGG + chr4_2:60973775-60973794 None:intergenic 30.0%
CATCACAGATAAACCTAAAA+AGG + chr4_2:60973913-60973932 None:intergenic 30.0%
! TTTAGGTTTATCTGTGATGT+TGG - chr4_2:60973914-60973933 Msa0612520:intron 30.0%
TTAGGTTTATCTGTGATGTT+GGG - chr4_2:60973915-60973934 Msa0612520:intron 30.0%
AGTACTACGTTAAAATATGG+AGG + chr4_2:60974340-60974359 None:intergenic 30.0%
AGCAGTACTACGTTAAAATA+TGG + chr4_2:60974343-60974362 None:intergenic 30.0%
ACTGCTACAGTATAAAAGTA+TGG - chr4_2:60974357-60974376 Msa0612520:intron 30.0%
! CTGCTACAGTATAAAAGTAT+GGG - chr4_2:60974358-60974377 Msa0612520:intron 30.0%
AATATCTCTAGATCGTAGTA+CGG - chr4_2:60974594-60974613 Msa0612520:intron 30.0%
ACAATGATTAAAGTTTCTCC+GGG + chr4_2:60974772-60974791 None:intergenic 30.0%
CGTTGTCATATCAATGTAAA+AGG + chr4_2:60974952-60974971 None:intergenic 30.0%
AAAATAACCGTTCTTATCAG+TGG + chr4_2:60975101-60975120 None:intergenic 30.0%
!!! GTTTTTTAGTGTTGAGAGAT+TGG - chr4_2:60975375-60975394 Msa0612520:CDS 30.0%
TCCACTTTCGTGTTAAATTA+TGG - chr4_2:60975400-60975419 Msa0612520:CDS 30.0%
ACCATAATTTAACACGAAAG+TGG + chr4_2:60975404-60975423 None:intergenic 30.0%
TCGTGTTAAATTATGGTGTT+TGG - chr4_2:60975407-60975426 Msa0612520:CDS 30.0%
! CAAGACAATTTTCTAAGTAC+AGG + chr4_2:60975547-60975566 None:intergenic 30.0%
!!! ACTATTTTAGTTGGGTATAC+GGG - chr4_2:60975583-60975602 Msa0612520:five_prime_UTR 30.0%
GAACAGCTATAAAGCTTCTA+GGG + chr4_2:60973475-60973494 None:intergenic 35.0%
TGAACAGCTATAAAGCTTCT+AGG + chr4_2:60973476-60973495 None:intergenic 35.0%
! AGAAGCTTTATAGCTGTTCA+AGG - chr4_2:60973476-60973495 Msa0612520:intron 35.0%
CCATTAATCTCGTAGATCTA+CGG + chr4_2:60973857-60973876 None:intergenic 35.0%
TAGGTTTATCTGTGATGTTG+GGG - chr4_2:60973916-60973935 Msa0612520:intron 35.0%
ATTCCCTAACATATGTCCTT+AGG - chr4_2:60974520-60974539 Msa0612520:intron 35.0%
TTCCCTAACATATGTCCTTA+GGG - chr4_2:60974521-60974540 Msa0612520:intron 35.0%
CGTAAAAAAGTTATGTTCCC+CGG - chr4_2:60974751-60974770 Msa0612520:intron 35.0%
CAATGATTAAAGTTTCTCCG+GGG + chr4_2:60974771-60974790 None:intergenic 35.0%
! GTGTTAAGTACTGTTAAGAG+AGG - chr4_2:60974821-60974840 Msa0612520:intron 35.0%
CTAAAACGACAAAGTAGCTT+TGG + chr4_2:60974885-60974904 None:intergenic 35.0%
!! AGTGTCGTTTAGCAACAAAA+AGG + chr4_2:60974982-60975001 None:intergenic 35.0%
CAACAACACAAAGTACAAGT+TGG - chr4_2:60975060-60975079 Msa0612520:CDS 35.0%
! TTCGAAGGCTTTAATCCATT+CGG + chr4_2:60975193-60975212 None:intergenic 35.0%
TCTTCCCTTTATTCTCTACA+CGG - chr4_2:60975273-60975292 Msa0612520:CDS 35.0%
! TTTTCTTAACGGGGACATTT+AGG + chr4_2:60975319-60975338 None:intergenic 35.0%
! GTGAGTGAGGGTTGATTTAT+AGG + chr4_2:60972896-60972915 None:intergenic 40.0%
! TTATGAGAGAGTGTGAGTGA+GGG + chr4_2:60972908-60972927 None:intergenic 40.0%
AAGTAACCCTAGAAAGAGAG+AGG + chr4_2:60972951-60972970 None:intergenic 40.0%
! AGCTATAAAGCTTCTAGGGA+GGG + chr4_2:60973471-60973490 None:intergenic 40.0%
GTCAAATCTTGCAAGTATGC+TGG - chr4_2:60973509-60973528 Msa0612520:intron 40.0%
!!! TGCTGGTGTTGATACTCTTT+TGG - chr4_2:60973526-60973545 Msa0612520:intron 40.0%
!!! GCTGGTGTTGATACTCTTTT+GGG - chr4_2:60973527-60973546 Msa0612520:intron 40.0%
TTGGGAGCTACTCAAATTCT+TGG - chr4_2:60973545-60973564 Msa0612520:intron 40.0%
CCGTAGATCTACGAGATTAA+TGG - chr4_2:60973854-60973873 Msa0612520:intron 40.0%
AGGTTTATCTGTGATGTTGG+GGG - chr4_2:60973917-60973936 Msa0612520:intron 40.0%
! TCTTGTTATCTTGTGCCCTT+AGG - chr4_2:60974381-60974400 Msa0612520:intron 40.0%
! CTTGTTATCTTGTGCCCTTA+GGG - chr4_2:60974382-60974401 Msa0612520:intron 40.0%
!! TTCCTTAACTTGTGCCCTAA+GGG + chr4_2:60974399-60974418 None:intergenic 40.0%
!! ATTCCTTAACTTGTGCCCTA+AGG + chr4_2:60974400-60974419 None:intergenic 40.0%
TGCCCTAAGGACATATGTTA+GGG + chr4_2:60974526-60974545 None:intergenic 40.0%
CAATGTTAACATGTGCCCTA+AGG + chr4_2:60974539-60974558 None:intergenic 40.0%
CTTAGGGCACATGTTAACAT+TGG - chr4_2:60974537-60974556 Msa0612520:intron 40.0%
ATCGTAGTACGGTAGTACTT+TGG - chr4_2:60974605-60974624 Msa0612520:intron 40.0%
TCGTAGTACGGTAGTACTTT+GGG - chr4_2:60974606-60974625 Msa0612520:intron 40.0%
TTGCAATTAAGGGAACAGTG+TGG - chr4_2:60974792-60974811 Msa0612520:intron 40.0%
AAGAGAGGAGAATCCAAGAA+TGG - chr4_2:60974836-60974855 Msa0612520:intron 40.0%
GTTGCATTGAAGCTTCACAA+CGG + chr4_2:60975044-60975063 None:intergenic 40.0%
!!! TATCAGTGGTCTGGGTTTTT+TGG + chr4_2:60975087-60975106 None:intergenic 40.0%
! GCATAGCTTGTGATGTTCTT+TGG + chr4_2:60975136-60975155 None:intergenic 40.0%
TTAAAGCCTTCGAACCTTCA+TGG - chr4_2:60975199-60975218 Msa0612520:CDS 40.0%
CCAACCGTGTAGAGAATAAA+GGG + chr4_2:60975280-60975299 None:intergenic 40.0%
TCCAACCGTGTAGAGAATAA+AGG + chr4_2:60975281-60975300 None:intergenic 40.0%
CATTTAGGCTCAAATGCAAG+AGG + chr4_2:60975304-60975323 None:intergenic 40.0%
! GTTATGAGAGAGTGTGAGTG+AGG + chr4_2:60972909-60972928 None:intergenic 45.0%
AGAGGAAGAGGATGTGATGT+TGG + chr4_2:60972933-60972952 None:intergenic 45.0%
TCCTCTTCCTCTCTCTTTCT+AGG - chr4_2:60972941-60972960 Msa0612520:three_prime_UTR 45.0%
CCTCTTCCTCTCTCTTTCTA+GGG - chr4_2:60972942-60972961 Msa0612520:three_prime_UTR 45.0%
TTGTGTGAGGAGGGTAAGTT+GGG + chr4_2:60973076-60973095 None:intergenic 45.0%
GTGTGAATAGGAGAAGGAGT+AGG + chr4_2:60973200-60973219 None:intergenic 45.0%
! AATGGTAAGGAGGGTGTAGA+GGG + chr4_2:60973223-60973242 None:intergenic 45.0%
CAGGTGTAGAATGGTAAGGA+GGG + chr4_2:60973232-60973251 None:intergenic 45.0%
ACAGGTGTAGAATGGTAAGG+AGG + chr4_2:60973233-60973252 None:intergenic 45.0%
GGTACAGGTGTAGAATGGTA+AGG + chr4_2:60973236-60973255 None:intergenic 45.0%
TAGCAGGTACAGGTGTAGAA+TGG + chr4_2:60973241-60973260 None:intergenic 45.0%
! TTGGAACAGGAGGGTGTAAT+GGG + chr4_2:60973394-60973413 None:intergenic 45.0%
! GCTATAAAGCTTCTAGGGAG+GGG + chr4_2:60973470-60973489 None:intergenic 45.0%
! CAGCTATAAAGCTTCTAGGG+AGG + chr4_2:60973472-60973491 None:intergenic 45.0%
GTGCCCTAAGGACATATGTT+AGG + chr4_2:60974527-60974546 None:intergenic 45.0%
AGTACGGTAGTACTTTGGGT+AGG - chr4_2:60974610-60974629 Msa0612520:intron 45.0%
GTACGGTAGTACTTTGGGTA+GGG - chr4_2:60974611-60974630 Msa0612520:intron 45.0%
TACGGTAGTACTTTGGGTAG+GGG - chr4_2:60974612-60974631 Msa0612520:intron 45.0%
ACCCAGACCACTGATAAGAA+CGG - chr4_2:60975091-60975110 Msa0612520:CDS 45.0%
ACCGTTCTTATCAGTGGTCT+GGG + chr4_2:60975095-60975114 None:intergenic 45.0%
AACCGTTCTTATCAGTGGTC+TGG + chr4_2:60975096-60975115 None:intergenic 45.0%
!! GTTTTAGTCAGTGCACCGAA+TGG - chr4_2:60975175-60975194 Msa0612520:CDS 45.0%
! AACCTTCATGGTGGTCTTAC+CGG - chr4_2:60975211-60975230 Msa0612520:CDS 45.0%
CCCTTTATTCTCTACACGGT+TGG - chr4_2:60975277-60975296 Msa0612520:CDS 45.0%
CCCTAGAAAGAGAGAGGAAG+AGG + chr4_2:60972945-60972964 None:intergenic 50.0%
TGTGTGAGGAGGGTAAGTTG+GGG + chr4_2:60973075-60973094 None:intergenic 50.0%
GTTGTGTGAGGAGGGTAAGT+TGG + chr4_2:60973077-60973096 None:intergenic 50.0%
!! AGTGAATGTGGTGGTGGTGA+TGG + chr4_2:60973163-60973182 None:intergenic 50.0%
TTGGTGAGTGAATGTGGTGG+TGG + chr4_2:60973169-60973188 None:intergenic 50.0%
TAGGAGAAGGAGTAGGAGCA+GGG + chr4_2:60973193-60973212 None:intergenic 50.0%
ATAGGAGAAGGAGTAGGAGC+AGG + chr4_2:60973194-60973213 None:intergenic 50.0%
! AGAGGGGTGTGAATAGGAGA+AGG + chr4_2:60973206-60973225 None:intergenic 50.0%
! ATGGTAAGGAGGGTGTAGAG+GGG + chr4_2:60973222-60973241 None:intergenic 50.0%
! GAATGGTAAGGAGGGTGTAG+AGG + chr4_2:60973224-60973243 None:intergenic 50.0%
!! GTTGGTGGTTTAGCAGGTAC+AGG + chr4_2:60973251-60973270 None:intergenic 50.0%
CCTGCTAAACCACCAACTCA+CGG - chr4_2:60973254-60973273 Msa0612520:CDS 50.0%
! GGGATGAGTTGGAACAACAG+GGG + chr4_2:60973372-60973391 None:intergenic 50.0%
! GGGGATGAGTTGGAACAACA+GGG + chr4_2:60973373-60973392 None:intergenic 50.0%
! TGGAACAGGAGGGTGTAATG+GGG + chr4_2:60973393-60973412 None:intergenic 50.0%
! GTTGGAACAGGAGGGTGTAA+TGG + chr4_2:60973395-60973414 None:intergenic 50.0%
! AATGGAGGGTGAGTTGGAAC+AGG + chr4_2:60973407-60973426 None:intergenic 50.0%
!! TGTGATGTTGGGGGTACTCT+TGG - chr4_2:60973926-60973945 Msa0612520:intron 50.0%
!! GTGATGTTGGGGGTACTCTT+GGG - chr4_2:60973927-60973946 Msa0612520:intron 50.0%
TGCCCTTAGGGCACAAGTTA+AGG - chr4_2:60974394-60974413 Msa0612520:intron 50.0%
AAGCCTTCGAACCTTCATGG+TGG - chr4_2:60975202-60975221 Msa0612520:CDS 50.0%
AGACCACCATGAAGGTTCGA+AGG + chr4_2:60975208-60975227 None:intergenic 50.0%
TTAGCCACAAACGGCTTCTC+GGG + chr4_2:60975250-60975269 None:intergenic 50.0%
CTTAGCCACAAACGGCTTCT+CGG + chr4_2:60975251-60975270 None:intergenic 50.0%
GAGAAGCCGTTTGTGGCTAA+GGG - chr4_2:60975250-60975269 Msa0612520:CDS 50.0%
GGAAGACCCTTAGCCACAAA+CGG + chr4_2:60975259-60975278 None:intergenic 50.0%
!! GAGCTGGTGTTGTGTGAGGA+GGG + chr4_2:60973085-60973104 None:intergenic 55.0%
!! AGTGGAGCTGGTGTTGTGTG+AGG + chr4_2:60973089-60973108 None:intergenic 55.0%
!! GACTTGGTTGGTGGGTGAAG+TGG + chr4_2:60973107-60973126 None:intergenic 55.0%
TCACCCACCAACCAAGTCAC+CGG - chr4_2:60973109-60973128 Msa0612520:three_prime_UTR 55.0%
!! GAACCGGTGACTTGGTTGGT+GGG + chr4_2:60973115-60973134 None:intergenic 55.0%
!! TGAACCGGTGACTTGGTTGG+TGG + chr4_2:60973116-60973135 None:intergenic 55.0%
!! GAATGTGGTGGTGGTGATGG+TGG + chr4_2:60973160-60973179 None:intergenic 55.0%
!! GGGTTGGTGAGTGAATGTGG+TGG + chr4_2:60973172-60973191 None:intergenic 55.0%
!! CAGGGGTTGGTGAGTGAATG+TGG + chr4_2:60973175-60973194 None:intergenic 55.0%
AGGAGAAGGAGTAGGAGCAG+GGG + chr4_2:60973192-60973211 None:intergenic 55.0%
! GGGTGTAGAGGGGTGTGAAT+AGG + chr4_2:60973212-60973231 None:intergenic 55.0%
!! CCGTGAGTTGGTGGTTTAGC+AGG + chr4_2:60973257-60973276 None:intergenic 55.0%
! AGCAACAGGTGTGTGGACTG+GGG + chr4_2:60973315-60973334 None:intergenic 55.0%
GAGCAACAGGTGTGTGGACT+GGG + chr4_2:60973316-60973335 None:intergenic 55.0%
TGAACAGGTGGGTGTAGTGG+TGG + chr4_2:60973347-60973366 None:intergenic 55.0%
GTGTGAACAGGTGGGTGTAG+TGG + chr4_2:60973350-60973369 None:intergenic 55.0%
! CAACAGGGGTGTGAACAGGT+GGG + chr4_2:60973358-60973377 None:intergenic 55.0%
! ACAACAGGGGTGTGAACAGG+TGG + chr4_2:60973359-60973378 None:intergenic 55.0%
! GGAACAACAGGGGTGTGAAC+AGG + chr4_2:60973362-60973381 None:intergenic 55.0%
! GGGGGATGAGTTGGAACAAC+AGG + chr4_2:60973374-60973393 None:intergenic 55.0%
! GGGTGTAATGGGGGATGAGT+TGG + chr4_2:60973383-60973402 None:intergenic 55.0%
! GGAACAGGAGGGTGTAATGG+GGG + chr4_2:60973392-60973411 None:intergenic 55.0%
! GAGGGTGAGTTGGAACAGGA+GGG + chr4_2:60973403-60973422 None:intergenic 55.0%
! GGGTGTAATGGAGGGTGAGT+TGG + chr4_2:60973413-60973432 None:intergenic 55.0%
! GAGCAGGTGGGTGTAATGGA+GGG + chr4_2:60973421-60973440 None:intergenic 55.0%
GGGGTGTAGGATGCAATGGT+GGG + chr4_2:60973451-60973470 None:intergenic 55.0%
! AGGGGTGTAGGATGCAATGG+TGG + chr4_2:60973452-60973471 None:intergenic 55.0%
! AAGCTTCTAGGGAGGGGTGT+AGG + chr4_2:60973464-60973483 None:intergenic 55.0%
CACCGGTAAGACCACCATGA+AGG + chr4_2:60975216-60975235 None:intergenic 55.0%
!! GTCTTACCGGTGCTGGTCTT+AGG - chr4_2:60975224-60975243 Msa0612520:CDS 55.0%
CGAGAAGCCGTTTGTGGCTA+AGG - chr4_2:60975249-60975268 Msa0612520:CDS 55.0%
!! GGAGCTGGTGTTGTGTGAGG+AGG + chr4_2:60973086-60973105 None:intergenic 60.0%
! GTTGGTGGGTGAAGTGGAGC+TGG + chr4_2:60973101-60973120 None:intergenic 60.0%
!! GGGTGAACCGGTGACTTGGT+TGG + chr4_2:60973119-60973138 None:intergenic 60.0%
!! CAAGTCACCGGTTCACCCAC+CGG - chr4_2:60973121-60973140 Msa0612520:three_prime_UTR 60.0%
!! GTGGTGGTGATGGTGGTGAG+GGG + chr4_2:60973153-60973172 None:intergenic 60.0%
!! GGTGGTGGTGATGGTGGTGA+GGG + chr4_2:60973154-60973173 None:intergenic 60.0%
!! TGGTGGTGGTGATGGTGGTG+AGG + chr4_2:60973155-60973174 None:intergenic 60.0%
GAAGGAGTAGGAGCAGGGGT+TGG + chr4_2:60973188-60973207 None:intergenic 60.0%
!! GGTGAGGATGATGGTGGTGG+TGG + chr4_2:60973280-60973299 None:intergenic 60.0%
!! GAGGGTGAGGATGATGGTGG+TGG + chr4_2:60973283-60973302 None:intergenic 60.0%
! CAGGAGGGTGAGGATGATGG+TGG + chr4_2:60973286-60973305 None:intergenic 60.0%
GAGCAGGAGGGTGAGGATGA+TGG + chr4_2:60973289-60973308 None:intergenic 60.0%
!! ACAGGTGTGTGGACTGGGGA+AGG + chr4_2:60973311-60973330 None:intergenic 60.0%
GGAGCAACAGGTGTGTGGAC+TGG + chr4_2:60973317-60973336 None:intergenic 60.0%
GTGCTGGAGCAACAGGTGTG+TGG + chr4_2:60973322-60973341 None:intergenic 60.0%
GGTGGATGTGCTGGAGCAAC+AGG + chr4_2:60973329-60973348 None:intergenic 60.0%
!! GGGTGTAGTGGTGGATGTGC+TGG + chr4_2:60973338-60973357 None:intergenic 60.0%
! GGAGGGTGAGTTGGAACAGG+AGG + chr4_2:60973404-60973423 None:intergenic 60.0%
GGAGCAGGTGGGTGTAATGG+AGG + chr4_2:60973422-60973441 None:intergenic 60.0%
GCTGGAGCAGGTGGGTGTAA+TGG + chr4_2:60973425-60973444 None:intergenic 60.0%
!! AATGGTGGGTGTGCTGGAGC+AGG + chr4_2:60973437-60973456 None:intergenic 60.0%
!! GGATGCAATGGTGGGTGTGC+TGG + chr4_2:60973443-60973462 None:intergenic 60.0%
! GGGAGGGGTGTAGGATGCAA+TGG + chr4_2:60973455-60973474 None:intergenic 60.0%
! CATGGTGGTCTTACCGGTGC+TGG - chr4_2:60975217-60975236 Msa0612520:CDS 60.0%
TAGGCCCGAGAAGCCGTTTG+TGG - chr4_2:60975243-60975262 Msa0612520:CDS 60.0%
CGGTGGGTGAACCGGTGACT+TGG + chr4_2:60973123-60973142 None:intergenic 65.0%
GCGTTAGCCGGTGGGTGAAC+CGG + chr4_2:60973131-60973150 None:intergenic 65.0%
!! TGGTGGTGAGGGGCGTTAGC+CGG + chr4_2:60973143-60973162 None:intergenic 65.0%
!! TGGTGGTGGCCGTGAGTTGG+TGG + chr4_2:60973266-60973285 None:intergenic 65.0%
!! TGGTGGTGGTGGCCGTGAGT+TGG + chr4_2:60973269-60973288 None:intergenic 65.0%
GGACTGGGGAAGGAGCAGGA+GGG + chr4_2:60973301-60973320 None:intergenic 65.0%
! TGGACTGGGGAAGGAGCAGG+AGG + chr4_2:60973302-60973321 None:intergenic 65.0%
!! GTGTGGACTGGGGAAGGAGC+AGG + chr4_2:60973305-60973324 None:intergenic 65.0%
GTGGGTGTGCTGGAGCAGGT+GGG + chr4_2:60973433-60973452 None:intergenic 65.0%
CTCGGGCCTAAGACCAGCAC+CGG + chr4_2:60975233-60975252 None:intergenic 65.0%
!! GGTGAGGGGCGTTAGCCGGT+GGG + chr4_2:60973139-60973158 None:intergenic 70.0%
!! TGGTGAGGGGCGTTAGCCGG+TGG + chr4_2:60973140-60973159 None:intergenic 70.0%
GGGGAAGGAGCAGGAGGGTG+AGG + chr4_2:60973296-60973315 None:intergenic 70.0%
GGTGGGTGTGCTGGAGCAGG+TGG + chr4_2:60973434-60973453 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 60972875 60975609 60972875 ID=Msa0612520;Name=Msa0612520
chr4_2 mRNA 60972875 60975609 60972875 ID=Msa0612520-mRNA-1;Parent=Msa0612520;Name=Msa0612520-mRNA-1;_AED=0.06;_eAED=0.06;_QI=115|1|1|1|1|1|2|276|290
chr4_2 exon 60972875 60973446 60972875 ID=Msa0612520-mRNA-1:exon:16463;Parent=Msa0612520-mRNA-1
chr4_2 exon 60974918 60975609 60974918 ID=Msa0612520-mRNA-1:exon:16462;Parent=Msa0612520-mRNA-1
chr4_2 five_prime_UTR 60975495 60975609 60975495 ID=Msa0612520-mRNA-1:five_prime_utr;Parent=Msa0612520-mRNA-1
chr4_2 CDS 60974918 60975494 60974918 ID=Msa0612520-mRNA-1:cds;Parent=Msa0612520-mRNA-1
chr4_2 CDS 60973151 60973446 60973151 ID=Msa0612520-mRNA-1:cds;Parent=Msa0612520-mRNA-1
chr4_2 three_prime_UTR 60972875 60973150 60972875 ID=Msa0612520-mRNA-1:three_prime_utr;Parent=Msa0612520-mRNA-1
Gene Sequence

>Msa0612520

ATGGCTAAAACCCTTGCAATATTGCTACTTTTACTTCAACTCACTTCTTTCACTGCATTTTCTGAAGAGCTTGAAACTCTTACCCCAACTTACCCTCCTCACACAACACCAGCTCCACTTCACCCACCAACCAAGTCACCGGTTCACCCACCGGCTAACGCCCCTCACCACCATCACCACCACCACATTCACTCACCAACCCCTGCTCCTACTCCTTCTCCTATTCACACCCCTCTACACCCTCCTTACCATTCTACACCTGTACCTGCTAAACCACCAACTCACGGCCACCACCACCATCATCCTCACCCTCCTGCTCCTTCCCCAGTCCACACACCTGTTGCTCCAGCACATCCACCACTACACCCACCTGTTCACACCCCTGTTGTTCCAACTCATCCCCCATTACACCCTCCTGTTCCAACTCACCCTCCATTACACCCACCTGCTCCAGCACACCCACCATTGCATCCTACACCCCTCCCTAGAAGCTTTATAGCTGTTCAAGGTGTTGTTTATGTCAAATCTTGCAAGTATGCTGGTGTTGATACTCTTTTGGGAGCTACTCAAATTCTTGGAGCCGTTGTGAAGCTTCAATGCAACAACACAAAGTACAAGTTGGTCCAAAAAACCCAGACCACTGATAAGAACGGTTATTTTTTCATTGAAGGACCAAAGAACATCACAAGCTATGCAGCTCATAAGTGCAACATTGTTTTAGTCAGTGCACCGAATGGATTAAAGCCTTCGAACCTTCATGGTGGTCTTACCGGTGCTGGTCTTAGGCCCGAGAAGCCGTTTGTGGCTAAGGGTCTTCCCTTTATTCTCTACACGGTTGGACCTCTTGCATTTGAGCCTAAATGTCCCCGTTAA
Protein sequence

>Msa0612520

MAKTLAILLLLLQLTSFTAFSEELETLTPTYPPHTTPAPLHPPTKSPVHPPANAPHHHHHHHIHSPTPAPTPSPIHTPLHPPYHSTPVPAKPPTHGHHHHHPHPPAPSPVHTPVAPAHPPLHPPVHTPVVPTHPPLHPPVPTHPPLHPPAPAHPPLHPTPLPRSFIAVQGVVYVKSCKYAGVDTLLGATQILGAVVKLQCNNTKYKLVQKTQTTDKNGYFFIEGPKNITSYAAHKCNIVLVSAPNGLKPSNLHGGLTGAGLRPEKPFVAKGLPFILYTVGPLAFEPKCPR*