AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0570010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0570010 MtrunA17_Chr8g0385291 82.194 556 41 9 1 551 1 503 0.0 889
Msa0570010 MtrunA17_Chr3g0131071 65.739 575 151 14 9 551 13 573 0.0 658
Msa0570010 MtrunA17_Chr4g0014901 68.992 387 111 5 165 545 207 590 0.0 554
Msa0570010 MtrunA17_Chr4g0014911 65.396 341 113 1 211 546 121 461 3.30e-160 464
Msa0570010 MtrunA17_Chr2g0293201 53.333 345 153 4 212 549 124 467 3.61e-130 388
Msa0570010 MtrunA17_Chr4g0004981 52.367 338 150 7 209 539 104 437 1.69e-117 355
Msa0570010 MtrunA17_Chr1g0183881 43.909 353 184 6 206 549 120 467 1.34e-99 309
Msa0570010 MtrunA17_Chr7g0243541 44.578 332 131 8 214 539 100 384 1.68e-87 276
Msa0570010 MtrunA17_Chr3g0131581 30.516 213 132 6 224 434 64 262 5.81e-19 87.0
Msa0570010 MtrunA17_Chr8g0385681 29.954 217 128 7 224 434 47 245 3.79e-16 78.6
Msa0570010 MtrunA17_Chr3g0144911 31.250 224 123 9 229 434 110 320 6.50e-16 80.1
Msa0570010 MtrunA17_Chr4g0035391 27.982 218 138 6 229 434 107 317 1.46e-15 79.7
Msa0570010 MtrunA17_Chr8g0385691 29.493 217 129 7 224 434 44 242 1.91e-15 76.3
Msa0570010 MtrunA17_Chr1g0155521 28.899 218 136 7 229 434 112 322 3.41e-15 78.6
Msa0570010 MtrunA17_Chr5g0404711 25.974 231 146 7 229 444 76 296 1.13e-14 76.6
Msa0570010 MtrunA17_Chr4g0035381 27.064 218 140 6 229 434 84 294 1.18e-14 75.9
Msa0570010 MtrunA17_Chr4g0035361 26.754 228 148 6 229 444 1 221 2.00e-13 72.8
Msa0570010 MtrunA17_Chr3g0144891 26.316 228 149 6 229 444 97 317 4.36e-13 72.0
Msa0570010 MtrunA17_Chr7g0236461 27.650 217 138 7 230 434 101 310 5.62e-13 71.6
Msa0570010 MtrunA17_Chr8g0372651 28.139 231 141 7 229 444 79 299 1.23e-12 70.5
Msa0570010 MtrunA17_Chr3g0141821 28.507 221 133 8 229 434 93 303 8.40e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0570010 AT4G09160.2 66.568 338 108 1 214 546 301 638 2.39e-172 502
Msa0570010 AT4G09160.3 66.568 338 108 1 214 546 301 638 2.94e-172 502
Msa0570010 AT4G09160.1 66.568 338 108 1 214 546 326 663 3.25e-172 503
Msa0570010 AT1G72160.1 66.082 342 111 1 210 546 147 488 1.23e-168 487
Msa0570010 AT1G22530.1 55.297 387 155 5 163 547 313 683 1.10e-144 433
Msa0570010 AT1G22530.2 55.297 387 155 5 163 547 279 649 2.00e-144 431
Msa0570010 AT1G72150.1 48.975 488 222 8 79 546 93 573 1.09e-142 424
Msa0570010 AT1G30690.1 48.986 345 163 7 212 550 202 539 2.90e-117 357
Msa0570010 AT1G30690.2 48.986 345 163 7 212 550 202 539 2.90e-117 357
Msa0570010 AT3G51670.1 47.872 376 185 8 180 548 34 405 6.72e-114 344
Msa0570010 AT1G01630.1 30.435 253 150 9 194 434 7 245 2.08e-18 85.5
Msa0570010 AT4G39180.3 29.817 218 134 6 229 434 96 306 1.81e-17 86.3
Msa0570010 AT4G39180.2 29.817 218 134 6 229 434 96 306 1.81e-17 86.3
Msa0570010 AT4G39180.1 29.817 218 134 6 229 434 96 306 1.90e-17 85.9
Msa0570010 AT2G21540.6 28.440 218 137 6 229 434 95 305 3.02e-16 82.4
Msa0570010 AT2G21540.2 28.440 218 137 6 229 434 95 305 3.02e-16 82.4
Msa0570010 AT2G21540.1 28.440 218 137 6 229 434 95 305 3.02e-16 82.4
Msa0570010 AT2G21540.4 28.440 218 137 6 229 434 95 305 3.02e-16 82.4
Msa0570010 AT2G21540.3 28.440 218 137 6 229 434 95 305 3.03e-16 82.0
Msa0570010 AT2G21540.5 28.440 218 137 6 229 434 95 305 3.03e-16 82.0
Msa0570010 AT4G34580.2 28.054 221 134 7 229 434 88 298 2.17e-15 79.7
Msa0570010 AT4G34580.1 28.054 221 134 7 229 434 88 298 2.17e-15 79.7
Msa0570010 AT3G24840.7 28.634 227 143 7 230 444 104 323 4.62e-15 78.6
Msa0570010 AT3G24840.3 28.634 227 143 7 230 444 104 323 4.62e-15 78.6
Msa0570010 AT3G24840.5 28.634 227 143 7 230 444 104 323 4.62e-15 78.6
Msa0570010 AT3G24840.1 28.634 227 143 7 230 444 104 323 5.26e-15 78.6
Msa0570010 AT3G24840.6 28.634 227 143 7 230 444 104 323 5.26e-15 78.6
Msa0570010 AT3G24840.4 28.634 227 143 7 230 444 104 323 5.26e-15 78.6
Msa0570010 AT3G24840.2 28.634 227 143 7 230 444 104 323 5.26e-15 78.6
Msa0570010 AT1G19650.1 28.571 231 140 7 229 444 104 324 8.89e-15 77.8
Msa0570010 AT2G16380.2 26.754 228 148 6 229 444 88 308 1.72e-14 76.6
Msa0570010 AT2G16380.1 26.754 228 148 6 229 444 88 308 1.72e-14 76.6
Msa0570010 AT1G19650.3 29.412 221 131 7 229 434 104 314 6.31e-14 74.3
Msa0570010 AT1G19650.2 28.261 230 140 7 230 444 33 252 1.36e-13 73.9
Msa0570010 AT4G39170.1 28.632 234 136 10 229 444 108 328 1.58e-13 73.9
Msa0570010 AT4G39170.2 28.632 234 136 10 229 444 108 328 1.71e-13 73.6
Msa0570010 AT2G21520.1 26.050 238 156 7 220 444 100 330 4.58e-13 72.4
Msa0570010 AT2G21520.2 26.050 238 156 7 220 444 104 334 5.02e-13 72.4
Msa0570010 AT2G21520.3 26.050 238 156 7 220 444 104 334 5.02e-13 72.4
Msa0570010 AT1G22180.2 26.818 220 141 5 226 441 45 248 1.10e-12 69.7
Msa0570010 AT1G22180.4 26.818 220 141 5 226 441 45 248 1.10e-12 69.7
Msa0570010 AT1G75370.1 26.754 228 148 7 229 444 113 333 1.31e-11 67.8
Msa0570010 AT4G08690.2 25.000 220 141 6 226 439 42 243 1.49e-11 65.9
Msa0570010 AT4G08690.1 25.000 220 141 6 226 439 42 243 1.49e-11 65.9
Msa0570010 AT4G08690.3 25.000 220 141 6 226 439 42 243 1.49e-11 65.9
Msa0570010 AT1G75170.2 25.701 214 138 6 226 434 43 240 5.00e-11 64.3
Msa0570010 AT1G75170.1 25.701 214 138 6 226 434 43 240 5.00e-11 64.3
Msa0570010 AT1G75370.2 27.193 228 147 7 229 444 113 333 6.26e-11 65.5

Find 169 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAACTGAATTCTCCATTTA+AGG 0.132695 4_1:-72038203 None:intergenic
TAATAGAGGTGATGGGTTTA+AGG 0.153149 4_1:-72037200 None:intergenic
CCTGAGGAAGTATCCATTTA+TGG 0.183327 4_1:+72037886 Msa0570010:CDS
TCTTGGGAGGTGAGAGTAAT+AGG 0.186017 4_1:+72039767 Msa0570010:CDS
AGAGACAAAATTTCCTTAAA+TGG 0.187206 4_1:+72038190 Msa0570010:CDS
TTGAGTAAAGATGGCGAATT+TGG 0.210654 4_1:+72038904 Msa0570010:CDS
GCAACAGGTTCTTGCTTTGC+AGG 0.224764 4_1:-72037832 None:intergenic
ACCTGTTTGGCCACAAATTC+GGG 0.225030 4_1:-72038375 None:intergenic
ATTCTCTGGAGAAAAGAATT+TGG 0.227723 4_1:+72038012 Msa0570010:CDS
ATTCTCCTGGACCTGGTAAA+TGG 0.228568 4_1:+72038301 Msa0570010:CDS
ACAAGCCCTTCAATTGTTTC+AGG 0.268118 4_1:+72038344 Msa0570010:CDS
TACCTGTTTGGCCACAAATT+CGG 0.279110 4_1:-72038376 None:intergenic
AGTTCCTTGAGAAGAGTATT+AGG 0.285523 4_1:+72038220 Msa0570010:CDS
CCATTGACAACACAAGTTCT+AGG 0.290988 4_1:+72039939 Msa0570010:CDS
AAAAGCAAGTTTGTCTTTGC+TGG 0.291845 4_1:+72038720 Msa0570010:CDS
ATTTGGAATAGAGGAGTTAA+TGG 0.294500 4_1:+72038029 Msa0570010:CDS
AAGGAGTGGTATCCCATAAA+TGG 0.296881 4_1:-72037899 None:intergenic
TGTTCATGTCAATGATCTTA+AGG 0.301789 4_1:+72038278 Msa0570010:CDS
ATCTTGTTGCTCTGACTCTA+AGG 0.326417 4_1:-72037362 None:intergenic
AGCAAAGACAAACTTGCTTT+TGG 0.331662 4_1:-72038718 None:intergenic
TGATGATGAATGAATATAAC+TGG 0.337085 4_1:-72037013 None:intergenic
TGCAGAAATGTATACTATCT+TGG 0.342464 4_1:+72039750 Msa0570010:intron
TCAGTCAGAGAGTGAAGATT+TGG 0.342948 4_1:-72039990 None:intergenic
CAAATTATCGGCGGTATTGC+CGG 0.349805 4_1:-72036989 None:intergenic
CAACAACATGTGGTGTTGTT+TGG 0.359368 4_1:-72037282 None:intergenic
CTATAAGAAAGAAGAGGATT+AGG 0.361078 4_1:+72036871 Msa0570010:five_prime_UTR
AATAGAGGTGATGGGTTTAA+GGG 0.362709 4_1:-72037199 None:intergenic
GTTTCAACTTTCTCTGCAAC+AGG 0.363178 4_1:-72037847 None:intergenic
AGTTCAATTTGAAGATCATT+TGG 0.363250 4_1:-72040104 None:intergenic
TTGTTCTTCGGTATTAGATT+TGG 0.364091 4_1:-72037440 None:intergenic
GAGTTAATGGATGAGAAATT+AGG 0.366666 4_1:+72038042 Msa0570010:CDS
AGGTTTGGTGGTTTGTTCTT+CGG 0.384117 4_1:-72037452 None:intergenic
AATATAACTGGGCAAATTAT+CGG 0.385445 4_1:-72037001 None:intergenic
GAGTAATAGGGTGGGAAGTA+AGG 0.385717 4_1:+72039780 Msa0570010:CDS
TGAGAAATTAGGTGATGAAT+TGG 0.386783 4_1:+72038053 Msa0570010:CDS
AGTTGAAAAGAGAGCTAAGT+TGG 0.387163 4_1:+72036898 Msa0570010:five_prime_UTR
AGACAATCAAATTACCTGTT+TGG 0.387293 4_1:-72038388 None:intergenic
CCCCGAATTTGTGGCCAAAC+AGG 0.390025 4_1:+72038374 Msa0570010:CDS
AAACAATTGAAGGGCTTGTT+TGG 0.391690 4_1:-72038340 None:intergenic
ATAATCAAACAAAACAATTT+CGG 0.397732 4_1:-72040059 None:intergenic
AGGAATAATGTGAAGAGAAA+AGG 0.397998 4_1:-72037228 None:intergenic
ACATGTGGTGTTGTTTGGTT+TGG 0.400074 4_1:-72037277 None:intergenic
GTTTGGAATATCAGAAGGTT+TGG 0.400595 4_1:-72037467 None:intergenic
TGCAGGTTCTTCTGGTGGGT+TGG 0.405196 4_1:-72037641 None:intergenic
CTGAGGAAGTATCCATTTAT+GGG 0.406828 4_1:+72037887 Msa0570010:CDS
CCCATGAAAATAGAACCTTT+TGG 0.416110 4_1:+72036948 Msa0570010:five_prime_UTR
AAAGGAAGAAGGAGATAAGA+AGG 0.419922 4_1:+72037708 Msa0570010:CDS
TTGGTTCTCTGGGTAAGAAA+AGG 0.421719 4_1:-72038699 None:intergenic
CTCAATCCACCGTACTTCAC+AGG 0.426324 4_1:-72038886 None:intergenic
GCAGAAATGTATACTATCTT+GGG 0.426741 4_1:+72039751 Msa0570010:intron
TGAGAAGAGTATTAGGAACT+TGG 0.426783 4_1:+72038227 Msa0570010:CDS
TTCTTATAGCTCATAGTATC+CGG 0.431052 4_1:-72036857 None:intergenic
CGTCACTTCTTTCGTCTACA+AGG 0.435281 4_1:-72037918 None:intergenic
AATCATCATGTTTGCACAAG+TGG 0.436481 4_1:+72040017 Msa0570010:three_prime_UTR
CTATACTGTGATTGTCCAAA+AGG 0.437811 4_1:+72039838 Msa0570010:CDS
TTCTCCTGGACCTGGTAAAT+GGG 0.441533 4_1:+72038302 Msa0570010:CDS
CCTAGAACTTGTGTTGTCAA+TGG 0.442818 4_1:-72039939 None:intergenic
AGTAGTAGGAAGGTGGAGCC+AGG 0.442834 4_1:-72037104 None:intergenic
CTGCTACTGAGATCACAATA+AGG 0.444194 4_1:+72038938 Msa0570010:CDS
GTATAGCTTCCTTCATTGCT+TGG 0.448666 4_1:-72039821 None:intergenic
GAGAAAAGAATTTGGAATAG+AGG 0.448687 4_1:+72038020 Msa0570010:CDS
TTACATGCATGGATTTGATA+AGG 0.449042 4_1:+72038086 Msa0570010:CDS
AGGTGATGAATTGGAGAAAG+TGG 0.450262 4_1:+72038062 Msa0570010:CDS
CAAAAGGGTCTCAGTTGACT+TGG 0.453511 4_1:-72038748 None:intergenic
CTTGTGCAGGTTCTTCTGGT+GGG 0.455127 4_1:-72037645 None:intergenic
AGTAGCTACCGTGGTGGTGG+TGG 0.457222 4_1:-72037587 None:intergenic
TTCCTTTCAATCATTCCAAA+AGG 0.458743 4_1:-72036963 None:intergenic
GAGAAAAGGAATTGTAATAG+AGG 0.461429 4_1:-72037214 None:intergenic
TGGTTCTCTGGGTAAGAAAA+GGG 0.464110 4_1:-72038698 None:intergenic
TACTTCACAGGGAGTTGCTC+TGG 0.465408 4_1:-72038874 None:intergenic
TGATTAAGAACACGATTCTC+TGG 0.467531 4_1:+72037998 Msa0570010:CDS
GGACAATTACCCCGAATTTG+TGG 0.468856 4_1:+72038365 Msa0570010:CDS
CCTGTGTGTTATAACATATA+TGG 0.470073 4_1:+72038117 Msa0570010:CDS
GATACTTCCTCAGGTGGTAA+TGG 0.483512 4_1:-72037877 None:intergenic
TCTTGTGCAGGTTCTTCTGG+TGG 0.483607 4_1:-72037646 None:intergenic
AGGAATTGTAATAGAGGTGA+TGG 0.486222 4_1:-72037208 None:intergenic
AAAGACGAAGAAAAGGAAGA+AGG 0.486264 4_1:+72037697 Msa0570010:CDS
AAAGCAAGTTTGTCTTTGCT+GGG 0.493026 4_1:+72038721 Msa0570010:CDS
AGATCATTTGGTGGTAAAAG+TGG 0.493558 4_1:-72040092 None:intergenic
CAATGTGCCATGGTGGTACT+TGG 0.493581 4_1:+72038656 Msa0570010:CDS
TGGAATATCAGAAGGTTTGG+TGG 0.495296 4_1:-72037464 None:intergenic
AATTGTCCTGAAACAATTGA+AGG 0.495434 4_1:-72038350 None:intergenic
AACGAAGAAAGACGAAGAAA+AGG 0.495758 4_1:+72037690 Msa0570010:CDS
CCATAAATGGATACTTCCTC+AGG 0.496479 4_1:-72037886 None:intergenic
TAAGAAGGAAGAGGAAGTGA+AGG 0.497688 4_1:+72037723 Msa0570010:CDS
TGTTGGCTGTAAGCATACTT+TGG 0.501286 4_1:-72036800 None:intergenic
CCTTCCTACTACTTCCAACC+TGG 0.501872 4_1:+72037114 Msa0570010:five_prime_UTR
AGTTCCCATTTACCAGGTCC+AGG 0.502724 4_1:-72038306 None:intergenic
CTTGGGAGGTGAGAGTAATA+GGG 0.506539 4_1:+72039768 Msa0570010:CDS
ATGAGTCTCAAGAGTGATGT+TGG 0.508469 4_1:-72037413 None:intergenic
AGAAGGAGATAAGAAGGAAG+AGG 0.510068 4_1:+72037714 Msa0570010:CDS
GAGAAAGTGGTTTACATGCA+TGG 0.510618 4_1:+72038075 Msa0570010:CDS
GGTGGTACTTGGCAGTGAAT+AGG 0.518645 4_1:+72038667 Msa0570010:CDS
TCAATTTGAAGATCATTTGG+TGG 0.520392 4_1:-72040101 None:intergenic
AAATAGAAGTTAACTAAAGC+AGG 0.521019 4_1:+72036921 Msa0570010:five_prime_UTR
TACGGTGGATTGAGTAAAGA+TGG 0.524873 4_1:+72038895 Msa0570010:CDS
TTGCAAAACAAACGTGGGAT+GGG 0.527633 4_1:-72037059 None:intergenic
TTTGGAATGATTGAAAGGAA+TGG 0.530582 4_1:+72036966 Msa0570010:five_prime_UTR
TGTCTAAGTTCCCATTTACC+AGG 0.530869 4_1:-72038312 None:intergenic
GTATCTTCTGTGGAGGATGA+TGG 0.535272 4_1:+72037754 Msa0570010:CDS
TGCAGTAGCTACCGTGGTGG+TGG 0.535296 4_1:-72037590 None:intergenic
GGTGATGGGTTTAAGGGGAA+AGG 0.537560 4_1:-72037193 None:intergenic
AATGATCTTAAGGATTCTCC+TGG 0.539991 4_1:+72038288 Msa0570010:CDS
GAATTTGTACCAAGCAATGA+AGG 0.540167 4_1:+72039812 Msa0570010:CDS
TGTAGAATTTCCTGTTACTG+AGG 0.540830 4_1:+72038978 Msa0570010:CDS
CTTAAGGATTCTCCTGGACC+TGG 0.542401 4_1:+72038294 Msa0570010:CDS
CCAACCAGGTTGGAAGTAGT+AGG 0.549032 4_1:-72037118 None:intergenic
ATGGTAAGAACAACTTTACC+AGG 0.550180 4_1:-72039920 None:intergenic
ACTCTATTGATATGAACAGC+AGG 0.550851 4_1:-72040129 None:intergenic
GAATGATTGAAAGGAATGGC+CGG 0.551815 4_1:+72036970 Msa0570010:five_prime_UTR
CCTACTACTTCCAACCTGGT+TGG 0.554708 4_1:+72037118 Msa0570010:five_prime_UTR
AGTTTCAAAATTAGTGAACC+TGG 0.558010 4_1:+72039902 Msa0570010:CDS
TTGGTGGAGCAGAGAAGGCA+TGG 0.561032 4_1:-72037546 None:intergenic
ATCGTGGGACATGTTGGAAG+AGG 0.563039 4_1:-72037248 None:intergenic
GTTGCAAAACAAACGTGGGA+TGG 0.563898 4_1:-72037060 None:intergenic
GTAGTAGGAAGGTGGAGCCA+GGG 0.564401 4_1:-72037103 None:intergenic
GGAATTGTAATAGAGGTGAT+GGG 0.567103 4_1:-72037207 None:intergenic
GAGCAACTCCCTGTGAAGTA+CGG 0.569298 4_1:+72038877 Msa0570010:CDS
AGAGTCATCGTGGGACATGT+TGG 0.571010 4_1:-72037254 None:intergenic
TCATTGTTTGGAATATCAGA+AGG 0.571936 4_1:-72037472 None:intergenic
AGGTGTTCATCAATGTGCCA+TGG 0.572219 4_1:+72038646 Msa0570010:intron
GGGAGGTGAGAGTAATAGGG+TGG 0.573952 4_1:+72039771 Msa0570010:CDS
GTGAGTATGTTGTGCCAACC+AGG 0.574096 4_1:-72037132 None:intergenic
TGTCCAAAAGGCAAGAAAAG+TGG 0.574530 4_1:+72039850 Msa0570010:CDS
ATGCATGGATTTGATAAGGA+AGG 0.579630 4_1:+72038090 Msa0570010:CDS
GGAAAATGTAGTATCTTCTG+TGG 0.580397 4_1:+72037744 Msa0570010:CDS
TCTTGTTGCTCTGACTCTAA+GGG 0.581269 4_1:-72037361 None:intergenic
GTAGCTACCGTGGTGGTGGT+GGG 0.586439 4_1:-72037586 None:intergenic
AGTTCCTAATACTCTTCTCA+AGG 0.586771 4_1:-72038224 None:intergenic
AGAAGTTAACTAAAGCAGGC+AGG 0.594282 4_1:+72036925 Msa0570010:five_prime_UTR
TTCATTGCCATTACCACCTG+AGG 0.595484 4_1:+72037870 Msa0570010:CDS
CTTCTTTCGTCTACAAGGAG+TGG 0.596620 4_1:-72037913 None:intergenic
AACAACACCACATGTTGTTG+AGG 0.598014 4_1:+72037285 Msa0570010:CDS
ATAGGGTGGGAAGTAAGGTA+TGG 0.600440 4_1:+72039785 Msa0570010:CDS
ATTGTCCTGAAACAATTGAA+GGG 0.601114 4_1:-72038349 None:intergenic
AGGGTCTCAGTTGACTTGGA+AGG 0.603122 4_1:-72038744 None:intergenic
CCATATATGTTATAACACAC+AGG 0.604070 4_1:-72038117 None:intergenic
TTGGTTTGGAGAGTCATCGT+GGG 0.606315 4_1:-72037263 None:intergenic
TTCAGGTGCAGTAGCTACCG+TGG 0.607269 4_1:-72037596 None:intergenic
TATGAGCTATAAGAAAGAAG+AGG 0.608629 4_1:+72036865 Msa0570010:five_prime_UTR
GGAGGTGAGAGTAATAGGGT+GGG 0.610127 4_1:+72039772 Msa0570010:CDS
ATAGAGGTGATGGGTTTAAG+GGG 0.610223 4_1:-72037198 None:intergenic
GGTTGGAAGTAGTAGGAAGG+TGG 0.614157 4_1:-72037111 None:intergenic
TAGTAGTTGCAAAACAAACG+TGG 0.615786 4_1:-72037065 None:intergenic
TAACATTGCAGTGCAGACCC+TGG 0.620062 4_1:+72037086 Msa0570010:five_prime_UTR
TCAATCCACCGTACTTCACA+GGG 0.625536 4_1:-72038885 None:intergenic
TTTGGTTTGGAGAGTCATCG+TGG 0.627279 4_1:-72037264 None:intergenic
AAATGTAGTATCTTCTGTGG+AGG 0.629752 4_1:+72037747 Msa0570010:CDS
AGAACCTGCACAAGAAAACA+AGG 0.630005 4_1:+72037654 Msa0570010:CDS
TTCTTGCTTTGCAGGAACAG+AGG 0.630059 4_1:-72037824 None:intergenic
TAAATGGATACTTCCTCAGG+TGG 0.634257 4_1:-72037883 None:intergenic
GTGAAGATTTGGTCTTCAAG+CGG 0.637718 4_1:-72039979 None:intergenic
CCAGGTTGGAAGTAGTAGGA+AGG 0.642316 4_1:-72037114 None:intergenic
GAAATGTATACTATCTTGGG+AGG 0.647101 4_1:+72039754 Msa0570010:intron
ACAACTTCCTCAACAACATG+TGG 0.651182 4_1:-72037292 None:intergenic
CCTGTTTGGCCACAAATTCG+GGG 0.652164 4_1:-72038374 None:intergenic
CATATATGTTATAACACACA+GGG 0.656526 4_1:-72038116 None:intergenic
AAAACAACCCACCACCACCA+CGG 0.657260 4_1:+72037579 Msa0570010:CDS
ATATGGTGAATTTCAGAACA+AGG 0.659457 4_1:+72038134 Msa0570010:CDS
CAACTCCCTGTGAAGTACGG+TGG 0.660112 4_1:+72038880 Msa0570010:CDS
AAGTGTCTAAATATGAGTGT+TGG 0.686179 4_1:-72036817 None:intergenic
TTCACTGCCAAGTACCACCA+TGG 0.690995 4_1:-72038663 None:intergenic
AGTAGTTGCAAAACAAACGT+GGG 0.697584 4_1:-72037064 None:intergenic
ATAACTGGGCAAATTATCGG+CGG 0.704638 4_1:-72036998 None:intergenic
AGGTGCAGTAGCTACCGTGG+TGG 0.704823 4_1:-72037593 None:intergenic
TGTTCATCAATGTGCCATGG+TGG 0.709016 4_1:+72038649 Msa0570010:CDS
GTATGTTGTGCCAACCAGGT+TGG 0.709233 4_1:-72037128 None:intergenic
CAATAGTGCATACACCACCA+TGG 0.712017 4_1:-72038257 None:intergenic
GATGATGAATGAATATAACT+GGG 0.716682 4_1:-72037012 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTACATACAATTTTTTTATA+TGG + chr4_1:72038563-72038582 Msa0570010:intron 10.0%
!!! GACTTTTTTTTTTTTTATTT+GGG - chr4_1:72039454-72039473 None:intergenic 10.0%
!!! TGACTTTTTTTTTTTTTATT+TGG - chr4_1:72039455-72039474 None:intergenic 10.0%
!!! TGCAATTTTTTGAAATATTA+AGG - chr4_1:72038518-72038537 None:intergenic 15.0%
!!! ATTGTGAATAATTTGATTTT+TGG + chr4_1:72038825-72038844 Msa0570010:intron 15.0%
!!! AGGTTTGATATTTTTTTATT+TGG + chr4_1:72039721-72039740 Msa0570010:intron 15.0%
!! ATAATCAAACAAAACAATTT+CGG - chr4_1:72040062-72040081 None:intergenic 15.0%
!!! TTCACTTTTTTCATATTTAC+CGG + chr4_1:72036838-72036857 Msa0570010:five_prime_UTR 20.0%
!! TTGTCTTCTTATAACATTAT+TGG + chr4_1:72038405-72038424 Msa0570010:intron 20.0%
!!! TTAACACTTTCTGTTTTTTT+AGG + chr4_1:72038626-72038645 Msa0570010:intron 20.0%
!! GGAATAGAATATAAAATCAA+AGG - chr4_1:72039103-72039122 None:intergenic 20.0%
!!! AATTTTTACAAGCATTAAAG+TGG - chr4_1:72039263-72039282 None:intergenic 20.0%
!!! TGATGTTTTGATTTCTTTAT+AGG + chr4_1:72039529-72039548 Msa0570010:intron 20.0%
!! AAATATCAAACCTTATATCA+AGG - chr4_1:72039714-72039733 None:intergenic 20.0%
! AAATAGAAGTTAACTAAAGC+AGG + chr4_1:72036921-72036940 Msa0570010:five_prime_UTR 25.0%
! AATATAACTGGGCAAATTAT+CGG - chr4_1:72037004-72037023 None:intergenic 25.0%
! GATGATGAATGAATATAACT+GGG - chr4_1:72037015-72037034 None:intergenic 25.0%
!! TGATGATGAATGAATATAAC+TGG - chr4_1:72037016-72037035 None:intergenic 25.0%
!! TGTTTTTCTTCTTTTATTGG+TGG - chr4_1:72037565-72037584 None:intergenic 25.0%
!!! GGTTGTTTTTCTTCTTTTAT+TGG - chr4_1:72037568-72037587 None:intergenic 25.0%
! CATATATGTTATAACACACA+GGG - chr4_1:72038119-72038138 None:intergenic 25.0%
! AGAGACAAAATTTCCTTAAA+TGG + chr4_1:72038190-72038209 Msa0570010:CDS 25.0%
! TTTGCTAAAAGAATATACGT+TGG - chr4_1:72038494-72038513 None:intergenic 25.0%
!! TATATGTTTTCACCTCAAAA+GGG - chr4_1:72038766-72038785 None:intergenic 25.0%
!! TTATATGTTTTCACCTCAAA+AGG - chr4_1:72038767-72038786 None:intergenic 25.0%
!!! TTTTGAGGTGAAAACATATA+AGG + chr4_1:72038766-72038785 Msa0570010:intron 25.0%
!! TTTGAGGTGAAAACATATAA+GGG + chr4_1:72038767-72038786 Msa0570010:intron 25.0%
!!! AGGGTTAAAAATTTGCTTTT+TGG + chr4_1:72038786-72038805 Msa0570010:intron 25.0%
! TAGGATTAAGAATCTTTCAT+AGG + chr4_1:72039041-72039060 Msa0570010:intron 25.0%
! TCAGATAATATTGACAAAAG+AGG - chr4_1:72039070-72039089 None:intergenic 25.0%
!! AGAGCATTCTACATATATAA+AGG + chr4_1:72039359-72039378 Msa0570010:intron 25.0%
! GAGCATTCTACATATATAAA+GGG + chr4_1:72039360-72039379 Msa0570010:intron 25.0%
! TGAAAACGACTTTAAACAAA+AGG + chr4_1:72039394-72039413 Msa0570010:intron 25.0%
! GACTTTAAACAAAAGGAAAA+AGG + chr4_1:72039401-72039420 Msa0570010:intron 25.0%
! ATCAAAACATCAACATCATT+AGG - chr4_1:72039521-72039540 None:intergenic 25.0%
! ATGTTAGAATTAGTTAGTGA+TGG + chr4_1:72039649-72039668 Msa0570010:intron 25.0%
! AATTCATTTGCCTTGATATA+AGG + chr4_1:72039701-72039720 Msa0570010:intron 25.0%
! AGTTCAATTTGAAGATCATT+TGG - chr4_1:72040107-72040126 None:intergenic 25.0%
AAGTGTCTAAATATGAGTGT+TGG - chr4_1:72036820-72036839 None:intergenic 30.0%
TTCTTATAGCTCATAGTATC+CGG - chr4_1:72036860-72036879 None:intergenic 30.0%
TATGAGCTATAAGAAAGAAG+AGG + chr4_1:72036865-72036884 Msa0570010:five_prime_UTR 30.0%
CTATAAGAAAGAAGAGGATT+AGG + chr4_1:72036871-72036890 Msa0570010:five_prime_UTR 30.0%
! CCAAAAGGTTCTATTTTCAT+GGG - chr4_1:72036951-72036970 None:intergenic 30.0%
! TCCAAAAGGTTCTATTTTCA+TGG - chr4_1:72036952-72036971 None:intergenic 30.0%
!!! AACCTTTTGGAATGATTGAA+AGG + chr4_1:72036961-72036980 Msa0570010:five_prime_UTR 30.0%
TTCCTTTCAATCATTCCAAA+AGG - chr4_1:72036966-72036985 None:intergenic 30.0%
! TTTGGAATGATTGAAAGGAA+TGG + chr4_1:72036966-72036985 Msa0570010:five_prime_UTR 30.0%
GAGAAAAGGAATTGTAATAG+AGG - chr4_1:72037217-72037236 None:intergenic 30.0%
AGGAATAATGTGAAGAGAAA+AGG - chr4_1:72037231-72037250 None:intergenic 30.0%
TTGTTCTTCGGTATTAGATT+TGG - chr4_1:72037443-72037462 None:intergenic 30.0%
! TCATTGTTTGGAATATCAGA+AGG - chr4_1:72037475-72037494 None:intergenic 30.0%
!!! AGTGACTTGTTTTCATTGTT+TGG - chr4_1:72037487-72037506 None:intergenic 30.0%
! GAATGAGAATTTTAAGCTCT+TGG - chr4_1:72037510-72037529 None:intergenic 30.0%
!! GAAAGTTGTTGTTCTTTTTC+AGG - chr4_1:72037616-72037635 None:intergenic 30.0%
!!! AAGAGATTTTAAGGTGAAAG+AGG + chr4_1:72037966-72037985 Msa0570010:CDS 30.0%
! ATTCTCTGGAGAAAAGAATT+TGG + chr4_1:72038012-72038031 Msa0570010:CDS 30.0%
! GAGAAAAGAATTTGGAATAG+AGG + chr4_1:72038020-72038039 Msa0570010:CDS 30.0%
! ATTTGGAATAGAGGAGTTAA+TGG + chr4_1:72038029-72038048 Msa0570010:CDS 30.0%
! GAGTTAATGGATGAGAAATT+AGG + chr4_1:72038042-72038061 Msa0570010:CDS 30.0%
TGAGAAATTAGGTGATGAAT+TGG + chr4_1:72038053-72038072 Msa0570010:CDS 30.0%
TTACATGCATGGATTTGATA+AGG + chr4_1:72038086-72038105 Msa0570010:CDS 30.0%
CCATATATGTTATAACACAC+AGG - chr4_1:72038120-72038139 None:intergenic 30.0%
CCTGTGTGTTATAACATATA+TGG + chr4_1:72038117-72038136 Msa0570010:CDS 30.0%
ATATGGTGAATTTCAGAACA+AGG + chr4_1:72038134-72038153 Msa0570010:CDS 30.0%
!! CAAGAAAACTTTTTCTGATG+AGG + chr4_1:72038164-72038183 Msa0570010:CDS 30.0%
TGTTCATGTCAATGATCTTA+AGG + chr4_1:72038278-72038297 Msa0570010:CDS 30.0%
ATTGTCCTGAAACAATTGAA+GGG - chr4_1:72038352-72038371 None:intergenic 30.0%
AATTGTCCTGAAACAATTGA+AGG - chr4_1:72038353-72038372 None:intergenic 30.0%
AGACAATCAAATTACCTGTT+TGG - chr4_1:72038391-72038410 None:intergenic 30.0%
GTTATTCACATTGGAATGAT+TGG - chr4_1:72038472-72038491 None:intergenic 30.0%
! TATACGTTGGTTATTCACAT+TGG - chr4_1:72038481-72038500 None:intergenic 30.0%
TAATCCTATGTTGCATTCTT+TGG - chr4_1:72039029-72039048 None:intergenic 30.0%
AGACTGTGAAAGAGATATAT+AGG - chr4_1:72039485-72039504 None:intergenic 30.0%
TATATATCTCTTTCACAGTC+TGG + chr4_1:72039484-72039503 Msa0570010:intron 30.0%
AAAACATCAACATCATTAGG+TGG - chr4_1:72039518-72039537 None:intergenic 30.0%
!! CTAACATTTTACACTAGTCT+AGG - chr4_1:72039636-72039655 None:intergenic 30.0%
TGCAGAAATGTATACTATCT+TGG + chr4_1:72039750-72039769 Msa0570010:intron 30.0%
GCAGAAATGTATACTATCTT+GGG + chr4_1:72039751-72039770 Msa0570010:intron 30.0%
AGTTTCAAAATTAGTGAACC+TGG + chr4_1:72039902-72039921 Msa0570010:CDS 30.0%
TCAATTTGAAGATCATTTGG+TGG - chr4_1:72040104-72040123 None:intergenic 30.0%
AGTTGAAAAGAGAGCTAAGT+TGG + chr4_1:72036898-72036917 Msa0570010:five_prime_UTR 35.0%
! CCCATGAAAATAGAACCTTT+TGG + chr4_1:72036948-72036967 Msa0570010:five_prime_UTR 35.0%
AGTAGTTGCAAAACAAACGT+GGG - chr4_1:72037067-72037086 None:intergenic 35.0%
TAGTAGTTGCAAAACAAACG+TGG - chr4_1:72037068-72037087 None:intergenic 35.0%
!! AATAGAGGTGATGGGTTTAA+GGG - chr4_1:72037202-72037221 None:intergenic 35.0%
!! TAATAGAGGTGATGGGTTTA+AGG - chr4_1:72037203-72037222 None:intergenic 35.0%
GGAATTGTAATAGAGGTGAT+GGG - chr4_1:72037210-72037229 None:intergenic 35.0%
AGGAATTGTAATAGAGGTGA+TGG - chr4_1:72037211-72037230 None:intergenic 35.0%
! TTTTCTTCTCAGGTTCATCA+GGG - chr4_1:72037393-72037412 None:intergenic 35.0%
! GTTTGGAATATCAGAAGGTT+TGG - chr4_1:72037470-72037489 None:intergenic 35.0%
AACGAAGAAAGACGAAGAAA+AGG + chr4_1:72037690-72037709 Msa0570010:CDS 35.0%
AAAGACGAAGAAAAGGAAGA+AGG + chr4_1:72037697-72037716 Msa0570010:CDS 35.0%
AAAGGAAGAAGGAGATAAGA+AGG + chr4_1:72037708-72037727 Msa0570010:CDS 35.0%
GGAAAATGTAGTATCTTCTG+TGG + chr4_1:72037744-72037763 Msa0570010:CDS 35.0%
AAATGTAGTATCTTCTGTGG+AGG + chr4_1:72037747-72037766 Msa0570010:CDS 35.0%
!! CTGAGGAAGTATCCATTTAT+GGG + chr4_1:72037887-72037906 Msa0570010:CDS 35.0%
! TCTACGTGCAAGAGATTTTA+AGG + chr4_1:72037957-72037976 Msa0570010:CDS 35.0%
TGATTAAGAACACGATTCTC+TGG + chr4_1:72037998-72038017 Msa0570010:CDS 35.0%
ATGCATGGATTTGATAAGGA+AGG + chr4_1:72038090-72038109 Msa0570010:CDS 35.0%
GGAACTGAATTCTCCATTTA+AGG - chr4_1:72038206-72038225 None:intergenic 35.0%
!! AGTTCCTTGAGAAGAGTATT+AGG + chr4_1:72038220-72038239 Msa0570010:CDS 35.0%
AGTTCCTAATACTCTTCTCA+AGG - chr4_1:72038227-72038246 None:intergenic 35.0%
!! TGAGAAGAGTATTAGGAACT+TGG + chr4_1:72038227-72038246 Msa0570010:CDS 35.0%
!! AGGAACTTGGATTTTAACCA+TGG + chr4_1:72038240-72038259 Msa0570010:CDS 35.0%
!! AACTTGGATTTTAACCATGG+TGG + chr4_1:72038243-72038262 Msa0570010:CDS 35.0%
AATGATCTTAAGGATTCTCC+TGG + chr4_1:72038288-72038307 Msa0570010:CDS 35.0%
! AAACAATTGAAGGGCTTGTT+TGG - chr4_1:72038343-72038362 None:intergenic 35.0%
!!! AAACTTGCTTTTGGTTCTCT+GGG - chr4_1:72038712-72038731 None:intergenic 35.0%
!! AGCAAAGACAAACTTGCTTT+TGG - chr4_1:72038721-72038740 None:intergenic 35.0%
AAAAGCAAGTTTGTCTTTGC+TGG + chr4_1:72038720-72038739 Msa0570010:CDS 35.0%
AAAGCAAGTTTGTCTTTGCT+GGG + chr4_1:72038721-72038740 Msa0570010:CDS 35.0%
TTGAGTAAAGATGGCGAATT+TGG + chr4_1:72038904-72038923 Msa0570010:CDS 35.0%
!!! TCTACAGTATGTTTTGATGC+TGG - chr4_1:72038964-72038983 None:intergenic 35.0%
TGTAGAATTTCCTGTTACTG+AGG + chr4_1:72038978-72038997 Msa0570010:CDS 35.0%
TTCTATTCCACAAATTGTGC+AGG + chr4_1:72039114-72039133 Msa0570010:intron 35.0%
!!! TTTTGTTAGCTTTGCAGTGT+TGG + chr4_1:72039229-72039248 Msa0570010:intron 35.0%
GAAATGTATACTATCTTGGG+AGG + chr4_1:72039754-72039773 Msa0570010:intron 35.0%
GAATTTGTACCAAGCAATGA+AGG + chr4_1:72039812-72039831 Msa0570010:CDS 35.0%
CTATACTGTGATTGTCCAAA+AGG + chr4_1:72039838-72039857 Msa0570010:CDS 35.0%
ATGGTAAGAACAACTTTACC+AGG - chr4_1:72039923-72039942 None:intergenic 35.0%
AATCATCATGTTTGCACAAG+TGG + chr4_1:72040017-72040036 Msa0570010:three_prime_UTR 35.0%
! AGATCATTTGGTGGTAAAAG+TGG - chr4_1:72040095-72040114 None:intergenic 35.0%
ACTCTATTGATATGAACAGC+AGG - chr4_1:72040132-72040151 None:intergenic 35.0%
! TGTTGGCTGTAAGCATACTT+TGG - chr4_1:72036803-72036822 None:intergenic 40.0%
AGAAGTTAACTAAAGCAGGC+AGG + chr4_1:72036925-72036944 Msa0570010:five_prime_UTR 40.0%
GAATGATTGAAAGGAATGGC+CGG + chr4_1:72036970-72036989 Msa0570010:five_prime_UTR 40.0%
ATAACTGGGCAAATTATCGG+CGG - chr4_1:72037001-72037020 None:intergenic 40.0%
TTGCAAAACAAACGTGGGAT+GGG - chr4_1:72037062-72037081 None:intergenic 40.0%
!! ATAGAGGTGATGGGTTTAAG+GGG - chr4_1:72037201-72037220 None:intergenic 40.0%
!! ACATGTGGTGTTGTTTGGTT+TGG - chr4_1:72037280-72037299 None:intergenic 40.0%
!! CAACAACATGTGGTGTTGTT+TGG - chr4_1:72037285-72037304 None:intergenic 40.0%
AACAACACCACATGTTGTTG+AGG + chr4_1:72037285-72037304 Msa0570010:CDS 40.0%
ACAACTTCCTCAACAACATG+TGG - chr4_1:72037295-72037314 None:intergenic 40.0%
!!! GTAGCTGTTTTTTCAGGTTG+GGG - chr4_1:72037337-72037356 None:intergenic 40.0%
!!! CGTAGCTGTTTTTTCAGGTT+GGG - chr4_1:72037338-72037357 None:intergenic 40.0%
!!! TCGTAGCTGTTTTTTCAGGT+TGG - chr4_1:72037339-72037358 None:intergenic 40.0%
!!! GGAATCGTAGCTGTTTTTTC+AGG - chr4_1:72037343-72037362 None:intergenic 40.0%
TCTTGTTGCTCTGACTCTAA+GGG - chr4_1:72037364-72037383 None:intergenic 40.0%
ATCTTGTTGCTCTGACTCTA+AGG - chr4_1:72037365-72037384 None:intergenic 40.0%
! GTTTTCTTCTCAGGTTCATC+AGG - chr4_1:72037394-72037413 None:intergenic 40.0%
!!! GTGATGTTGGTTTTCTTCTC+AGG - chr4_1:72037403-72037422 None:intergenic 40.0%
ATGAGTCTCAAGAGTGATGT+TGG - chr4_1:72037416-72037435 None:intergenic 40.0%
!! AGGTTTGGTGGTTTGTTCTT+CGG - chr4_1:72037455-72037474 None:intergenic 40.0%
TGGAATATCAGAAGGTTTGG+TGG - chr4_1:72037467-72037486 None:intergenic 40.0%
! TTTTATTGGTGGAGCAGAGA+AGG - chr4_1:72037554-72037573 None:intergenic 40.0%
! TTTTCTTGTGCAGGTTCTTC+TGG - chr4_1:72037652-72037671 None:intergenic 40.0%
AGAACCTGCACAAGAAAACA+AGG + chr4_1:72037654-72037673 Msa0570010:CDS 40.0%
AGAAGGAGATAAGAAGGAAG+AGG + chr4_1:72037714-72037733 Msa0570010:CDS 40.0%
TAAGAAGGAAGAGGAAGTGA+AGG + chr4_1:72037723-72037742 Msa0570010:CDS 40.0%
GTTTCAACTTTCTCTGCAAC+AGG - chr4_1:72037850-72037869 None:intergenic 40.0%
TAAATGGATACTTCCTCAGG+TGG - chr4_1:72037886-72037905 None:intergenic 40.0%
CCATAAATGGATACTTCCTC+AGG - chr4_1:72037889-72037908 None:intergenic 40.0%
!! CCTGAGGAAGTATCCATTTA+TGG + chr4_1:72037886-72037905 Msa0570010:CDS 40.0%
AAGGAGTGGTATCCCATAAA+TGG - chr4_1:72037902-72037921 None:intergenic 40.0%
AGGTGATGAATTGGAGAAAG+TGG + chr4_1:72038062-72038081 Msa0570010:CDS 40.0%
GAGAAAGTGGTTTACATGCA+TGG + chr4_1:72038075-72038094 Msa0570010:CDS 40.0%
TGTCTAAGTTCCCATTTACC+AGG - chr4_1:72038315-72038334 None:intergenic 40.0%
ACAAGCCCTTCAATTGTTTC+AGG + chr4_1:72038344-72038363 Msa0570010:CDS 40.0%
TACCTGTTTGGCCACAAATT+CGG - chr4_1:72038379-72038398 None:intergenic 40.0%
TGGTTCTCTGGGTAAGAAAA+GGG - chr4_1:72038701-72038720 None:intergenic 40.0%
! TTGGTTCTCTGGGTAAGAAA+AGG - chr4_1:72038702-72038721 None:intergenic 40.0%
!!! CAAACTTGCTTTTGGTTCTC+TGG - chr4_1:72038713-72038732 None:intergenic 40.0%
!! TACGGTGGATTGAGTAAAGA+TGG + chr4_1:72038895-72038914 Msa0570010:CDS 40.0%
CTGCTACTGAGATCACAATA+AGG + chr4_1:72038938-72038957 Msa0570010:CDS 40.0%
GAATTTCCTGTTACTGAGGT+TGG + chr4_1:72038982-72039001 Msa0570010:intron 40.0%
GAAAAACCAACCTCAGTAAC+AGG - chr4_1:72038991-72039010 None:intergenic 40.0%
GCATCCAAAGAATGCAACAT+AGG + chr4_1:72039022-72039041 Msa0570010:intron 40.0%
CATCAACATCATTAGGTGGT+TGG - chr4_1:72039514-72039533 None:intergenic 40.0%
GTATAGCTTCCTTCATTGCT+TGG - chr4_1:72039824-72039843 None:intergenic 40.0%
TGTCCAAAAGGCAAGAAAAG+TGG + chr4_1:72039850-72039869 Msa0570010:CDS 40.0%
!!! AAGCCACTTTTCTTGCCTTT+TGG - chr4_1:72039856-72039875 None:intergenic 40.0%
CCTAGAACTTGTGTTGTCAA+TGG - chr4_1:72039942-72039961 None:intergenic 40.0%
CCATTGACAACACAAGTTCT+AGG + chr4_1:72039939-72039958 Msa0570010:CDS 40.0%
! GTGAAGATTTGGTCTTCAAG+CGG - chr4_1:72039982-72040001 None:intergenic 40.0%
TCAGTCAGAGAGTGAAGATT+TGG - chr4_1:72039993-72040012 None:intergenic 40.0%
CAAATTATCGGCGGTATTGC+CGG - chr4_1:72036992-72037011 None:intergenic 45.0%
GTTGCAAAACAAACGTGGGA+TGG - chr4_1:72037063-72037082 None:intergenic 45.0%
!! TTGGTTTGGAGAGTCATCGT+GGG - chr4_1:72037266-72037285 None:intergenic 45.0%
!! TTTGGTTTGGAGAGTCATCG+TGG - chr4_1:72037267-72037286 None:intergenic 45.0%
!!! TCGTCCTTGTTTTCTTGTGC+AGG - chr4_1:72037661-72037680 None:intergenic 45.0%
GTATCTTCTGTGGAGGATGA+TGG + chr4_1:72037754-72037773 Msa0570010:CDS 45.0%
!! GGATGATGGAGCAAAAACTG+TGG + chr4_1:72037768-72037787 Msa0570010:CDS 45.0%
TGATGGAGCAAAAACTGTGG+AGG + chr4_1:72037771-72037790 Msa0570010:CDS 45.0%
TTCTTGCTTTGCAGGAACAG+AGG - chr4_1:72037827-72037846 None:intergenic 45.0%
TTCATTGCCATTACCACCTG+AGG + chr4_1:72037870-72037889 Msa0570010:CDS 45.0%
GATACTTCCTCAGGTGGTAA+TGG - chr4_1:72037880-72037899 None:intergenic 45.0%
CTTCTTTCGTCTACAAGGAG+TGG - chr4_1:72037916-72037935 None:intergenic 45.0%
CGTCACTTCTTTCGTCTACA+AGG - chr4_1:72037921-72037940 None:intergenic 45.0%
CAATAGTGCATACACCACCA+TGG - chr4_1:72038260-72038279 None:intergenic 45.0%
ATTCTCCTGGACCTGGTAAA+TGG + chr4_1:72038301-72038320 Msa0570010:CDS 45.0%
TTCTCCTGGACCTGGTAAAT+GGG + chr4_1:72038302-72038321 Msa0570010:CDS 45.0%
GGACAATTACCCCGAATTTG+TGG + chr4_1:72038365-72038384 Msa0570010:CDS 45.0%
ACCTGTTTGGCCACAAATTC+GGG - chr4_1:72038378-72038397 None:intergenic 45.0%
AGGTGTTCATCAATGTGCCA+TGG + chr4_1:72038646-72038665 Msa0570010:intron 45.0%
TGTTCATCAATGTGCCATGG+TGG + chr4_1:72038649-72038668 Msa0570010:CDS 45.0%
CAAAAGGGTCTCAGTTGACT+TGG - chr4_1:72038751-72038770 None:intergenic 45.0%
! AGTCAACTGAGACCCTTTTG+AGG + chr4_1:72038751-72038770 Msa0570010:CDS 45.0%
TCAATCCACCGTACTTCACA+GGG - chr4_1:72038888-72038907 None:intergenic 45.0%
CTTCAAGCCTGCACAATTTG+TGG - chr4_1:72039124-72039143 None:intergenic 45.0%
TCTTGGGAGGTGAGAGTAAT+AGG + chr4_1:72039767-72039786 Msa0570010:CDS 45.0%
CTTGGGAGGTGAGAGTAATA+GGG + chr4_1:72039768-72039787 Msa0570010:CDS 45.0%
GAGTAATAGGGTGGGAAGTA+AGG + chr4_1:72039780-72039799 Msa0570010:CDS 45.0%
ATAGGGTGGGAAGTAAGGTA+TGG + chr4_1:72039785-72039804 Msa0570010:CDS 45.0%
TAACATTGCAGTGCAGACCC+TGG + chr4_1:72037086-72037105 Msa0570010:five_prime_UTR 50.0%
GGTTGGAAGTAGTAGGAAGG+TGG - chr4_1:72037114-72037133 None:intergenic 50.0%
! CCAGGTTGGAAGTAGTAGGA+AGG - chr4_1:72037117-72037136 None:intergenic 50.0%
CCTTCCTACTACTTCCAACC+TGG + chr4_1:72037114-72037133 Msa0570010:five_prime_UTR 50.0%
! CCAACCAGGTTGGAAGTAGT+AGG - chr4_1:72037121-72037140 None:intergenic 50.0%
CCTACTACTTCCAACCTGGT+TGG + chr4_1:72037118-72037137 Msa0570010:five_prime_UTR 50.0%
GTATGTTGTGCCAACCAGGT+TGG - chr4_1:72037131-72037150 None:intergenic 50.0%
GTGAGTATGTTGTGCCAACC+AGG - chr4_1:72037135-72037154 None:intergenic 50.0%
!! GGTGATGGGTTTAAGGGGAA+AGG - chr4_1:72037196-72037215 None:intergenic 50.0%
ATCGTGGGACATGTTGGAAG+AGG - chr4_1:72037251-72037270 None:intergenic 50.0%
AGAGTCATCGTGGGACATGT+TGG - chr4_1:72037257-72037276 None:intergenic 50.0%
AAAACAACCCACCACCACCA+CGG + chr4_1:72037579-72037598 Msa0570010:CDS 50.0%
CTTGTGCAGGTTCTTCTGGT+GGG - chr4_1:72037648-72037667 None:intergenic 50.0%
TCTTGTGCAGGTTCTTCTGG+TGG - chr4_1:72037649-72037668 None:intergenic 50.0%
GCAACAGGTTCTTGCTTTGC+AGG - chr4_1:72037835-72037854 None:intergenic 50.0%
CTTAAGGATTCTCCTGGACC+TGG + chr4_1:72038294-72038313 Msa0570010:CDS 50.0%
AGTTCCCATTTACCAGGTCC+AGG - chr4_1:72038309-72038328 None:intergenic 50.0%
CCTGTTTGGCCACAAATTCG+GGG - chr4_1:72038377-72038396 None:intergenic 50.0%
CAATGTGCCATGGTGGTACT+TGG + chr4_1:72038656-72038675 Msa0570010:CDS 50.0%
TTCACTGCCAAGTACCACCA+TGG - chr4_1:72038666-72038685 None:intergenic 50.0%
GGTGGTACTTGGCAGTGAAT+AGG + chr4_1:72038667-72038686 Msa0570010:CDS 50.0%
!! AGGGTCTCAGTTGACTTGGA+AGG - chr4_1:72038747-72038766 None:intergenic 50.0%
TACTTCACAGGGAGTTGCTC+TGG - chr4_1:72038877-72038896 None:intergenic 50.0%
GAGCAACTCCCTGTGAAGTA+CGG + chr4_1:72038877-72038896 Msa0570010:CDS 50.0%
CTCAATCCACCGTACTTCAC+AGG - chr4_1:72038889-72038908 None:intergenic 50.0%
! AGCTTTGCAGTGTTGGACTG+TGG + chr4_1:72039236-72039255 Msa0570010:intron 50.0%
GGAGGTGAGAGTAATAGGGT+GGG + chr4_1:72039772-72039791 Msa0570010:CDS 50.0%
GTAGTAGGAAGGTGGAGCCA+GGG - chr4_1:72037106-72037125 None:intergenic 55.0%
AGTAGTAGGAAGGTGGAGCC+AGG - chr4_1:72037107-72037126 None:intergenic 55.0%
TTGGTGGAGCAGAGAAGGCA+TGG - chr4_1:72037549-72037568 None:intergenic 55.0%
TTCAGGTGCAGTAGCTACCG+TGG - chr4_1:72037599-72037618 None:intergenic 55.0%
! TGCAGGTTCTTCTGGTGGGT+TGG - chr4_1:72037644-72037663 None:intergenic 55.0%
CCCCGAATTTGTGGCCAAAC+AGG + chr4_1:72038374-72038393 Msa0570010:CDS 55.0%
CAACTCCCTGTGAAGTACGG+TGG + chr4_1:72038880-72038899 Msa0570010:CDS 55.0%
GGGAGGTGAGAGTAATAGGG+TGG + chr4_1:72039771-72039790 Msa0570010:CDS 55.0%
!! GTAGCTACCGTGGTGGTGGT+GGG - chr4_1:72037589-72037608 None:intergenic 60.0%
!! AGTAGCTACCGTGGTGGTGG+TGG - chr4_1:72037590-72037609 None:intergenic 60.0%
TGCAGTAGCTACCGTGGTGG+TGG - chr4_1:72037593-72037612 None:intergenic 60.0%
AGGTGCAGTAGCTACCGTGG+TGG - chr4_1:72037596-72037615 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 72036779 72040203 72036779 ID=Msa0570010;Name=Msa0570010
chr4_1 mRNA 72036779 72040203 72036779 ID=Msa0570010-mRNA-1;Parent=Msa0570010;Name=Msa0570010-mRNA-1;_AED=0.02;_eAED=0.02;_QI=480|1|1|1|1|1|4|191|551
chr4_1 exon 72036779 72038395 72036779 ID=Msa0570010-mRNA-1:exon:24530;Parent=Msa0570010-mRNA-1
chr4_1 exon 72038648 72038772 72038648 ID=Msa0570010-mRNA-1:exon:24531;Parent=Msa0570010-mRNA-1
chr4_1 exon 72038864 72038999 72038864 ID=Msa0570010-mRNA-1:exon:24532;Parent=Msa0570010-mRNA-1
chr4_1 exon 72039755 72040203 72039755 ID=Msa0570010-mRNA-1:exon:24533;Parent=Msa0570010-mRNA-1
chr4_1 five_prime_UTR 72036779 72037258 72036779 ID=Msa0570010-mRNA-1:five_prime_utr;Parent=Msa0570010-mRNA-1
chr4_1 CDS 72037259 72038395 72037259 ID=Msa0570010-mRNA-1:cds;Parent=Msa0570010-mRNA-1
chr4_1 CDS 72038648 72038772 72038648 ID=Msa0570010-mRNA-1:cds;Parent=Msa0570010-mRNA-1
chr4_1 CDS 72038864 72038999 72038864 ID=Msa0570010-mRNA-1:cds;Parent=Msa0570010-mRNA-1
chr4_1 CDS 72039755 72040012 72039755 ID=Msa0570010-mRNA-1:cds;Parent=Msa0570010-mRNA-1
chr4_1 three_prime_UTR 72040013 72040203 72040013 ID=Msa0570010-mRNA-1:three_prime_utr;Parent=Msa0570010-mRNA-1
Gene Sequence

>Msa0570010

ATGTCCCACGATGACTCTCCAAACCAAACAACACCACATGTTGTTGAGGAAGTTGTTGTTACTCATGTAAATGTTCCCCAACCTGAAAAAACAGCTACGATTCCCTTAGAGTCAGAGCAACAAGATTCACACCCTGATGAACCTGAGAAGAAAACCAACATCACTCTTGAGACTCATCTCTCCAAATCTAATACCGAAGAACAAACCACCAAACCTTCTGATATTCCAAACAATGAAAACAAGTCACTCCAAGAGCTTAAAATTCTCATTCAACAAGCACTCAATAACCATGCCTTCTCTGCTCCACCAATAAAAGAAGAAAAACAACCCACCACCACCACGGTAGCTACTGCACCTGAAAAAGAACAACAACTTTCTGATGCCAACCCACCAGAAGAACCTGCACAAGAAAACAAGGACGATAAAGTTGCAACGAAGAAAGACGAAGAAAAGGAAGAAGGAGATAAGAAGGAAGAGGAAGTGAAGGAAAATGTAGTATCTTCTGTGGAGGATGATGGAGCAAAAACTGTGGAGGCTATTGAAGAAAGCGTTGTTGCTGTTTCTGCCTCTGTTCCTGCAAAGCAAGAACCTGTTGCAGAGAAAGTTGAAACTTCATTGCCATTACCACCTGAGGAAGTATCCATTTATGGGATACCACTCCTTGTAGACGAAAGAAGTGACGTAATTCTGTTGAAATTTCTACGTGCAAGAGATTTTAAGGTGAAAGAGGCTTTCACAATGATTAAGAACACGATTCTCTGGAGAAAAGAATTTGGAATAGAGGAGTTAATGGATGAGAAATTAGGTGATGAATTGGAGAAAGTGGTTTACATGCATGGATTTGATAAGGAAGGTCACCCTGTGTGTTATAACATATATGGTGAATTTCAGAACAAGGAATTGTACAAGAAAACTTTTTCTGATGAGGAGAAGAGACAAAATTTCCTTAAATGGAGAATTCAGTTCCTTGAGAAGAGTATTAGGAACTTGGATTTTAACCATGGTGGTGTATGCACTATTGTTCATGTCAATGATCTTAAGGATTCTCCTGGACCTGGTAAATGGGAACTTAGACAAGCTACCAAACAAGCCCTTCAATTGTTTCAGGACAATTACCCCGAATTTGTGGCCAAACAGGTGTTCATCAATGTGCCATGGTGGTACTTGGCAGTGAATAGGATGATAAGCCCTTTTCTTACCCAGAGAACCAAAAGCAAGTTTGTCTTTGCTGGGCCTTCCAAGTCAACTGAGACCCTTTTGAGCTACATAGCTCCAGAGCAACTCCCTGTGAAGTACGGTGGATTGAGTAAAGATGGCGAATTTGGAAATTCAGATTCTGCTACTGAGATCACAATAAGGCCAGCATCAAAACATACTGTAGAATTTCCTGTTACTGAGAAATGTATACTATCTTGGGAGGTGAGAGTAATAGGGTGGGAAGTAAGGTATGGTGCAGAATTTGTACCAAGCAATGAAGGAAGCTATACTGTGATTGTCCAAAAGGCAAGAAAAGTGGCTTCATCAGAAGAAGCAGTGCTTTGCAACAGTTTCAAAATTAGTGAACCTGGTAAAGTTGTTCTTACCATTGACAACACAAGTTCTAGGAAGAAAAAGCTCTTGTACCGCTTGAAGACCAAATCTTCACTCTCTGACTGA
Protein sequence

>Msa0570010

MSHDDSPNQTTPHVVEEVVVTHVNVPQPEKTATIPLESEQQDSHPDEPEKKTNITLETHLSKSNTEEQTTKPSDIPNNENKSLQELKILIQQALNNHAFSAPPIKEEKQPTTTTVATAPEKEQQLSDANPPEEPAQENKDDKVATKKDEEKEEGDKKEEEVKENVVSSVEDDGAKTVEAIEESVVAVSASVPAKQEPVAEKVETSLPLPPEEVSIYGIPLLVDERSDVILLKFLRARDFKVKEAFTMIKNTILWRKEFGIEELMDEKLGDELEKVVYMHGFDKEGHPVCYNIYGEFQNKELYKKTFSDEEKRQNFLKWRIQFLEKSIRNLDFNHGGVCTIVHVNDLKDSPGPGKWELRQATKQALQLFQDNYPEFVAKQVFINVPWWYLAVNRMISPFLTQRTKSKFVFAGPSKSTETLLSYIAPEQLPVKYGGLSKDGEFGNSDSATEITIRPASKHTVEFPVTEKCILSWEVRVIGWEVRYGAEFVPSNEGSYTVIVQKARKVASSEEAVLCNSFKISEPGKVVLTIDNTSSRKKKLLYRLKTKSSLSD*