AlfalfaGEDB | Msa0716150

M. sativa cultivar zhongmu-4 / Msa0716150

KEGG: K20858
GO: GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006810 GO:0008150 GO:0015291 GO:0015292 GO:0022804 GO:0022857 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234 GO:0055085
PFAM: PF04678.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0716150	XP_003611914.3	97.384	344	8	1	6	349	1	343	0.0	690

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0716150	sp\|O64823\|MCU2_ARATH	49.107	336	143	7	6	328	1	321	1.78e-110	328

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0716150	G7KAV9	97.384	344	8	1	6	349	1	343	0.0	690

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0312890	Msa0716150	0.824945	6.129317e-54	-8.615850e-47
Msa0312950	Msa0716150	0.807829	4.173997e-50	-8.615850e-47
Msa0312970	Msa0716150	0.809023	2.322594e-50	-8.615850e-47
Msa0474570	Msa0716150	0.803125	4.047093e-49	-8.615850e-47
Msa0612590	Msa0716150	0.844679	6.512301e-59	-8.615850e-47
Msa0695650	Msa0716150	0.826309	2.911585e-54	-8.615850e-47
Msa0716150	Msa0798790	0.909715	4.189626e-82	-8.615850e-47
Msa0716150	Msa0837320	0.875119	3.961793e-68	-8.615850e-47
Msa0716150	Msa0888980	0.825075	5.711358e-54	-8.615850e-47
Msa0716150	Msa1158620	0.815068	1.117420e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0716150	MtrunA17_Chr8g0373631	54.324	370	130	8	6	348	1	358	5.07e-132	381
Msa0716150	MtrunA17_Chr5g0403551	93.939	198	11	1	1	198	19	215	9.47e-122	349
Msa0716150	MtrunA17_Chr5g0403541	98.462	130	2	0	220	349	1	130	1.09e-93	275
Msa0716150	MtrunA17_Chr1g0154611	41.265	332	182	5	6	330	1	326	1.29e-82	254
Msa0716150	MtrunA17_Chr3g0108861	34.247	219	142	1	115	331	99	317	3.04e-45	157
Msa0716150	MtrunA17_Chr5g0433141	33.023	215	142	1	115	327	92	306	1.90e-41	147

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0716150	AT2G23790.1	49.107	336	143	7	6	328	1	321	1.81e-111	328
Msa0716150	AT4G36820.1	46.407	334	153	6	8	329	4	323	1.70e-101	303
Msa0716150	AT5G66650.1	45.732	328	163	4	6	331	1	315	2.20e-96	289
Msa0716150	AT5G42610.1	43.511	262	142	4	70	326	21	281	1.30e-71	225
Msa0716150	AT1G57610.2	35.071	211	132	4	119	325	46	255	3.95e-40	143
Msa0716150	AT1G57610.1	35.071	211	132	4	119	325	46	255	3.95e-40	143
Msa0716150	AT1G09575.1	31.858	226	150	3	104	326	36	260	2.15e-36	133
Msa0716150	AT1G09575.2	31.858	226	150	3	104	326	36	260	2.15e-36	133
Msa0716150	AT1G57610.3	34.783	161	100	4	119	275	46	205	8.16e-24	97.8
Msa0716150	AT1G57610.4	34.783	161	100	4	119	275	46	205	8.16e-24	97.8

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Find 76 sgRNAs with CRISPR-Local

Find 286 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAGTTGCGTAACGGTTT+CGG	0.307406	5_1:-9559429	Msa0716150:CDS
AAATAATAATTTACCTGTTC+AGG	0.280313	5_1:+9559290	None:intergenic
AAGAAAAGAATACGAAGAAA+TGG	0.402281	5_1:-9558343	Msa0716150:CDS
AAGCAGACTTTGGAGTGTTT+TGG	0.306984	5_1:+9558398	None:intergenic
AAGCTGAAAACAATGGACAA+TGG	0.660072	5_1:-9559605	Msa0716150:CDS
AAGGAGGACGGTGTTAGCGT+CGG	0.634588	5_1:-9559518	Msa0716150:CDS
AAGGTTCTTTCCCGGTCTTG+AGG	0.616769	5_1:+9558127	None:intergenic
AATTAGAGATGGTGAAATCA+AGG	0.549065	5_1:-9559466	Msa0716150:CDS
ACAATGGTAGGAATCGTATT+CGG	0.527920	5_1:-9559589	Msa0716150:CDS
ACATCCATGTACTTCATGAC+TGG	0.497631	5_1:-9558162	Msa0716150:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGATGTGAAGAAATTGTTGA+AGG	-	chr5_1:9558363-9558382	Msa0716150:CDS	30.0%
AATTAGAGATGGTGAAATCA+AGG	-	chr5_1:9558392-9558411	Msa0716150:CDS	30.0%
AAATGGTGAAGATCAAAGAT+AGG	-	chr5_1:9558716-9558735	Msa0716150:intron	30.0%
ACCATTAATTCAAAGTTAGG+GGG	-	chr5_1:9559010-9559029	Msa0716150:intron	30.0%
TCATCTAAACATACACTTGA+TGG	-	chr5_1:9559267-9559286	Msa0716150:intron	30.0%
AGAAATGGAGAAAAAGAAGT+TGG	-	chr5_1:9559530-9559549	Msa0716150:CDS	30.0%
GGATGTTAAATAGAAGCAAA+TGG	+	chr5_1:9559682-9559701	None:intergenic	30.0%
CAAAATTGTGAAGCTTCATA+AGG	+	chr5_1:9559803-9559822	None:intergenic	30.0%
AGATGTGAAGAAATTGTTGA+AGG	-	chr5_1:9558363-9558382	Msa0716150:CDS	30.0%
AATTAGAGATGGTGAAATCA+AGG	-	chr5_1:9558392-9558411	Msa0716150:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	9557954	9559926	9557954	ID=Msa0716150;Name=Msa0716150
chr5_1	mRNA	9557954	9559926	9557954	ID=Msa0716150-mRNA-1;Parent=Msa0716150;Name=Msa0716150-mRNA-1;_AED=0.06;_eAED=0.06;_QI=44\|0\|0.5\|1\|1\|1\|2\|0\|349
chr5_1	exon	9557954	9558424	9557954	ID=Msa0716150-mRNA-1:exon:3822;Parent=Msa0716150-mRNA-1
chr5_1	exon	9559304	9559926	9559304	ID=Msa0716150-mRNA-1:exon:3821;Parent=Msa0716150-mRNA-1
chr5_1	five_prime_UTR	9559883	9559926	9559883	ID=Msa0716150-mRNA-1:five_prime_utr;Parent=Msa0716150-mRNA-1
chr5_1	CDS	9559304	9559882	9559304	ID=Msa0716150-mRNA-1:cds;Parent=Msa0716150-mRNA-1
chr5_1	CDS	9557954	9558424	9557954	ID=Msa0716150-mRNA-1:cds;Parent=Msa0716150-mRNA-1

Gene Sequence

>Msa0716150

ATGAATTTATCATCAATGGCGTTTAAGAGAACGCTTGCACAACGGTTATTCAATATAACCAAAATCTCATCACAAAATCTCACGAATTGTCGCATTTCATCTTCCTCCGTTAGTGTTTCAAAATCCGAATCTGACATTTCGCCTGAACCAGGCGATAATGGAGCATTCAAAAGATTCATTCACAAGCGAACCGGTTTTGAACCGAAACAACAAACCGGGTTCAAATCAGAGCTTCATCAACCAAACCTGATGGAGAAGCTGAAAACAATGGACAATGGTAGGAATCGTATTCGGTTGGATGGATTGATCATCAAAACGCCGGCGGAGAAACCGGCGACGCCGAAGGAGGACGGTGTTAGCGTCGGAGATGTGAAGAAATTGTTGAAGGTGGCGCAATTAGAGATGGTGAAATCAAGGCTGAGAGAATCATCAAAGAGTTGCGTAACGGTTTCGGAATTGATTCGAATTTGTTCTGAATATTGTTCTGATCATGATCAAGCGGTTAAGATTGTGAAGATGCTTGATGATTCTGCGGCTGTGATTATCTTAGGAGATGTTGTTTTTCTTAGACCTGAACAGGTAGCCAAAACACTCCAAAGTCTGCTTCCTACACCGGGAATAAAGGTATCAGATTCAGTAAGAAAAGAATACGAAGAAATGGAGAAAAAGAAGTTGGCCATCGACGAAGAAGCCGAAGCCTTGGTTGATCGTGAGCTTTGGGGAGGTTTGGGTTTCATTATGGTGAAAACAGCAGTATTAATGAGACTCACATTTTGGGAGCTTACTTGGGATGTAATGGAACCCATTTGCTTCTATTTAACATCCATGTACTTCATGACTGGTTATACATTCTTCCTCAAGACCGGGAAAGAACCTTCTTTTAGAGGTTTTTACGAAGCTCGTTTCAGCTCGAAGCAGAAGCGCCTTATGAAGCTTCACAATTTTGACATTGAAAAGTACAATAAGCTTAGCAACGTTTGTTCTCCCAATGTTCCTCCTATGTTTGATTCCTCCACTGCTCTCACATCTGAAAATTCTTTACATCACTGA

Protein sequence

>Msa0716150

MNLSSMAFKRTLAQRLFNITKISSQNLTNCRISSSSVSVSKSESDISPEPGDNGAFKRFIHKRTGFEPKQQTGFKSELHQPNLMEKLKTMDNGRNRIRLDGLIIKTPAEKPATPKEDGVSVGDVKKLLKVAQLEMVKSRLRESSKSCVTVSELIRICSEYCSDHDQAVKIVKMLDDSAAVIILGDVVFLRPEQVAKTLQSLLPTPGIKVSDSVRKEYEEMEKKKLAIDEEAEALVDRELWGGLGFIMVKTAVLMRLTFWELTWDVMEPICFYLTSMYFMTGYTFFLKTGKEPSFRGFYEARFSSKQKRLMKLHNFDIEKYNKLSNVCSPNVPPMFDSSTALTSENSLHH*