AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0695650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0695650 MtrunA17_Chr4g0047231 94.136 324 17 1 1 322 1 324 0.0 636
Msa0695650 MtrunA17_Chr4g0047221 84.568 324 46 3 1 322 55 376 0.0 574
Msa0695650 MtrunA17_Chr2g0282301 56.748 326 128 7 1 322 1 317 3.31e-126 363
Msa0695650 MtrunA17_Chr2g0282311 48.428 318 155 4 6 322 10 319 7.93e-108 317
Msa0695650 MtrunA17_Chr4g0040171 53.719 242 101 6 83 322 58 290 6.67e-85 257
Msa0695650 MtrunA17_Chr5g0396281 35.500 200 124 3 91 288 44 240 2.95e-43 149
Msa0695650 MtrunA17_Chr7g0251511 34.641 153 98 2 145 296 129 280 1.30e-32 123
Msa0695650 MtrunA17_Chr4g0075591 37.589 141 81 2 138 278 110 243 2.04e-31 119
Msa0695650 MtrunA17_Chr1g0166241 37.008 127 74 2 147 273 153 273 4.08e-30 117
Msa0695650 MtrunA17_Chr8g0339051 36.296 135 85 1 145 278 129 263 4.36e-30 116
Msa0695650 MtrunA17_Chr4g0075611 34.177 158 96 2 121 278 112 261 4.94e-30 115
Msa0695650 MtrunA17_Chr1g0184881 26.978 278 153 8 62 308 81 339 1.02e-27 110
Msa0695650 MtrunA17_Chr2g0289701 34.641 153 91 3 147 296 134 280 1.19e-27 109
Msa0695650 MtrunA17_Chr7g0260551 33.333 165 95 3 114 278 136 285 4.74e-27 108
Msa0695650 MtrunA17_Chr1g0188091 36.220 127 75 1 145 271 173 293 6.13e-27 108
Msa0695650 MtrunA17_Chr7g0234691 37.037 135 83 2 145 278 169 302 8.43e-27 107
Msa0695650 MtrunA17_Chr8g0384101 33.871 124 81 1 149 271 84 207 6.87e-26 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0695650 AT4G32480.1 47.080 274 124 6 36 308 21 274 4.46e-82 250
Msa0695650 AT2G20670.1 47.748 222 112 3 89 308 61 280 1.74e-73 229
Msa0695650 AT3G07350.1 40.909 132 78 0 147 278 135 266 9.64e-38 136
Msa0695650 AT4G14620.1 39.062 128 72 1 151 278 147 268 1.42e-32 124
Msa0695650 AT3G25240.1 31.466 232 147 5 68 291 30 257 1.79e-32 122
Msa0695650 AT2G38820.2 34.783 161 89 5 111 271 133 277 7.91e-31 118
Msa0695650 AT3G22970.2 37.879 132 75 2 148 278 57 182 3.46e-30 115
Msa0695650 AT2G39650.1 28.723 188 126 2 90 277 61 240 4.32e-30 116
Msa0695650 AT2G39650.2 30.220 182 119 2 96 277 5 178 4.67e-30 114
Msa0695650 AT3G22970.1 38.462 130 73 2 148 276 168 291 6.17e-29 114
Msa0695650 AT3G54550.1 30.769 143 89 2 148 286 145 281 3.75e-25 102
Msa0695650 AT1G62420.1 31.655 139 88 2 149 285 110 243 3.42e-24 100
Msa0695650 AT1G12030.1 32.061 131 82 2 149 278 112 236 6.59e-23 96.7
Msa0695650 AT1G77160.2 29.412 136 84 2 148 271 26 161 5.44e-20 87.0
Msa0695650 AT1G77145.1 29.167 144 90 2 148 279 76 219 8.37e-20 87.4
Msa0695650 AT1G77160.1 29.412 136 84 2 148 271 81 216 1.29e-19 87.0
Msa0695650 AT1G77145.2 32.710 107 68 1 177 279 56 162 3.27e-15 73.6

Find 56 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAAGAGAAAGGAGATATT+AGG 0.188794 4_4:-65276200 Msa0695650:CDS
AGTGGTTAGGTCCATTTAAA+AGG 0.261572 4_4:-65275963 Msa0695650:CDS
TTCAACTAATTCACTTTCAT+TGG 0.273663 4_4:-65275935 Msa0695650:CDS
GTGCAATTTGTAATACTAAA+TGG 0.278765 4_4:-65276844 Msa0695650:CDS
GATGCCTTTGGCTTCTGCTT+TGG 0.309443 4_4:+65275889 None:intergenic
GATGATTGTGATGTGGGTTT+TGG 0.352300 4_4:-65277367 Msa0695650:CDS
CAACAGAACTTGTTCTGTAT+AGG 0.355728 4_4:+65277023 None:intergenic
CAAAGAGAAAGGAGATATTA+GGG 0.363806 4_4:-65276199 Msa0695650:CDS
TTATGTGTATGGCAGCTAAA+AGG 0.369972 4_4:-65276047 Msa0695650:CDS
ATAGCAGTACAATGCATATC+AGG 0.379730 4_4:+65275835 None:intergenic
ACAGTTAACATCGATGCCTT+TGG 0.407087 4_4:+65275877 None:intergenic
ACTTGTCTCATAAAACAGTT+TGG 0.416082 4_4:+65276899 None:intergenic
TTTCTTGATGATTGTGATGT+GGG 0.416102 4_4:-65277373 Msa0695650:CDS
TCAACTAATTCACTTTCATT+GGG 0.420472 4_4:-65275934 Msa0695650:CDS
CATAATCAAGATTATGTGTA+TGG 0.427742 4_4:-65276058 Msa0695650:CDS
GTCAAAACATAACATAATCA+TGG 0.437841 4_4:-65277512 Msa0695650:exon
CCTAGTCACTCGTGCTAAAG+AGG 0.444536 4_4:+65277483 None:intergenic
TTACCTGCAAAAGCTGAAGT+TGG 0.467608 4_4:+65277212 None:intergenic
AAAACAGTTTGGACACGATC+TGG 0.468895 4_4:+65276910 None:intergenic
CTTTCGAGCACAATTTGAGA+TGG 0.476688 4_4:-65276160 Msa0695650:CDS
GGCAGCTAAAAGGTGTTTGA+AGG 0.493144 4_4:-65276037 Msa0695650:CDS
TCTAACCAGCTTCAAAATGC+TGG 0.495680 4_4:-65276875 Msa0695650:CDS
CAATATGCAGACACAAATCT+TGG 0.504940 4_4:-65277412 Msa0695650:CDS
GTTTCATCATGATAATCATA+TGG 0.507505 4_4:-65277323 Msa0695650:CDS
AGTTCACATAATCTTCCATC+AGG 0.512882 4_4:-65276818 Msa0695650:intron
TTTCGAGCACAATTTGAGAT+GGG 0.513578 4_4:-65276159 Msa0695650:CDS
GAATTCAAAGCAGGTTCAAA+CGG 0.514136 4_4:+65275660 None:intergenic
GTTCTGTTGAAATAAGCATA+AGG 0.516383 4_4:-65277009 Msa0695650:CDS
TACATGCAAGCTAAGTGGTT+AGG 0.517313 4_4:-65275976 Msa0695650:CDS
GTGACTAGGTATTTGCCAGC+TGG 0.519709 4_4:-65277469 Msa0695650:CDS
TCATGATAATCATATGGAGT+TGG 0.523947 4_4:-65277317 Msa0695650:CDS
TGATCTTGGGAATTCAAAGC+AGG 0.531761 4_4:+65275651 None:intergenic
CCTCTTTAGCACGAGTGACT+AGG 0.537423 4_4:-65277483 Msa0695650:CDS
AATTATTTATAGCAATACAT+AGG 0.537770 4_4:-65275683 Msa0695650:three_prime_UTR
TGAGATGGGAAAAGCAAGTG+AGG 0.544475 4_4:-65276145 Msa0695650:CDS
AAACTCCCTGAGGTTTATGT+AGG 0.545128 4_4:-65276102 Msa0695650:CDS
CATAATACAAGCAAAGAGAA+AGG 0.545468 4_4:-65276210 Msa0695650:CDS
TGCAACGAAAGAAGCAATTG+AGG 0.553944 4_4:-65276984 Msa0695650:CDS
TCAAAACATAACATAATCAT+GGG 0.557183 4_4:-65277511 Msa0695650:exon
TCTAGTGAGAGGGTTAAAGA+AGG 0.564021 4_4:-65277271 Msa0695650:CDS
TGTAGTTGTAGTAGACAAAG+TGG 0.573858 4_4:-65276938 Msa0695650:CDS
ATAAATACATGCAAGCTAAG+TGG 0.576303 4_4:-65275981 Msa0695650:CDS
ATGAGGATAAATCTAGTGAG+AGG 0.582076 4_4:-65277282 Msa0695650:CDS
TATTTCTAGTGAGAAAGGTG+TGG 0.607699 4_4:-65275738 Msa0695650:three_prime_UTR
ACAAAATGCACATGGGACCA+TGG 0.611544 4_4:-65276011 Msa0695650:CDS
TAATCCAAAGCAGAAGCCAA+AGG 0.630360 4_4:-65275893 Msa0695650:CDS
TAAAGAAGGTTGTAACAATG+AGG 0.631226 4_4:-65277257 Msa0695650:CDS
GTTGGATATTGAAGATGATG+AGG 0.631925 4_4:-65277299 Msa0695650:CDS
CAGGGAGTTTCCTCACAAGA+CGG 0.633524 4_4:+65276115 None:intergenic
GAAGGAAAACAAAATGCACA+TGG 0.638628 4_4:-65276019 Msa0695650:CDS
AATTCCCTACGAAAACCAGC+TGG 0.645424 4_4:+65277454 None:intergenic
TGAGGATAAATCTAGTGAGA+GGG 0.649115 4_4:-65277281 Msa0695650:CDS
AGGATTATAACCGTCTTGTG+AGG 0.653130 4_4:-65276125 Msa0695650:CDS
AAAGGCATCGATGTTAACTG+TGG 0.685100 4_4:-65275875 Msa0695650:CDS
AAGGAAAACAAAATGCACAT+GGG 0.713631 4_4:-65276018 Msa0695650:CDS
TCTTGTGAGGAAACTCCCTG+AGG 0.737856 4_4:-65276112 Msa0695650:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTTTAGTGATTTAAAA+GGG + chr4_4:65276601-65276620 None:intergenic 10.0%
!!! AAATATTTTAGTGATTTAAA+AGG + chr4_4:65276602-65276621 None:intergenic 10.0%
!!! AATATTTTAGTGATTTAAAA+GGG + chr4_4:65276601-65276620 None:intergenic 10.0%
!!! AAATATTTTAGTGATTTAAA+AGG + chr4_4:65276602-65276621 None:intergenic 10.0%
!!! TATTTTCTTAATCAATTTTG+AGG - chr4_4:65276035-65276054 Msa0695650:CDS 15.0%
!!! TATTTTCTTAATCAATTTTG+AGG - chr4_4:65276035-65276054 Msa0695650:CDS 15.0%
!! ACAGGATTATTAAAAAATTA+AGG - chr4_4:65276738-65276757 Msa0695650:intron 15.0%
!! TCAACAATAACAAAAAATTA+AGG - chr4_4:65276777-65276796 Msa0695650:intron 15.0%
!!! TGATACTATTTTTTGTTTTT+TGG - chr4_4:65277385-65277404 Msa0695650:CDS 15.0%
!! AATTATTTATAGCAATACAT+AGG - chr4_4:65277499-65277518 Msa0695650:exon 15.0%
!!! TATTTTCTTAATCAATTTTG+AGG - chr4_4:65276035-65276054 Msa0695650:CDS 15.0%
!!! TATTTTCTTAATCAATTTTG+AGG - chr4_4:65276035-65276054 Msa0695650:CDS 15.0%
!! ACAGGATTATTAAAAAATTA+AGG - chr4_4:65276738-65276757 Msa0695650:intron 15.0%
!! TCAACAATAACAAAAAATTA+AGG - chr4_4:65276777-65276796 Msa0695650:intron 15.0%
!!! TGATACTATTTTTTGTTTTT+TGG - chr4_4:65277385-65277404 Msa0695650:CDS 15.0%
!! AATTATTTATAGCAATACAT+AGG - chr4_4:65277499-65277518 Msa0695650:exon 15.0%
!! TCAAAACATAACATAATCAT+GGG - chr4_4:65275671-65275690 Msa0695650:three_prime_UTR 20.0%
!! AATTAAATGAAATTACCTGA+TGG + chr4_4:65276382-65276401 None:intergenic 20.0%
!! TAATACATTCTTGTCAATAT+AGG + chr4_4:65276664-65276683 None:intergenic 20.0%
!! TATATTGACAAGAATGTATT+AGG - chr4_4:65276663-65276682 Msa0695650:intron 20.0%
!!! TAAAAAATTAAGGTGTTATG+TGG - chr4_4:65276748-65276767 Msa0695650:intron 20.0%
!!! TAACAAAAAATTAAGGTGTT+AGG - chr4_4:65276784-65276803 Msa0695650:intron 20.0%
!! ATATGTGATTATAACTATAC+AGG - chr4_4:65276924-65276943 Msa0695650:CDS 20.0%
!! TATGTGATTATAACTATACA+GGG - chr4_4:65276925-65276944 Msa0695650:CDS 20.0%
!!! ATTTTTTGTTTTTTGGTAGA+TGG - chr4_4:65277392-65277411 Msa0695650:CDS 20.0%
!!! TTTTTTGTTTTTTGGTAGAT+GGG - chr4_4:65277393-65277412 Msa0695650:CDS 20.0%
!!! TTTTTTATTTCTAGTGAGAA+AGG - chr4_4:65277439-65277458 Msa0695650:CDS 20.0%
!! TCAAAACATAACATAATCAT+GGG - chr4_4:65275671-65275690 Msa0695650:three_prime_UTR 20.0%
!! AATTAAATGAAATTACCTGA+TGG + chr4_4:65276382-65276401 None:intergenic 20.0%
!! TAATACATTCTTGTCAATAT+AGG + chr4_4:65276664-65276683 None:intergenic 20.0%
!! TATATTGACAAGAATGTATT+AGG - chr4_4:65276663-65276682 Msa0695650:intron 20.0%
!!! TAAAAAATTAAGGTGTTATG+TGG - chr4_4:65276748-65276767 Msa0695650:intron 20.0%
!!! TAACAAAAAATTAAGGTGTT+AGG - chr4_4:65276784-65276803 Msa0695650:intron 20.0%
!! ATATGTGATTATAACTATAC+AGG - chr4_4:65276924-65276943 Msa0695650:CDS 20.0%
!! TATGTGATTATAACTATACA+GGG - chr4_4:65276925-65276944 Msa0695650:CDS 20.0%
!!! ATTTTTTGTTTTTTGGTAGA+TGG - chr4_4:65277392-65277411 Msa0695650:CDS 20.0%
!!! TTTTTTGTTTTTTGGTAGAT+GGG - chr4_4:65277393-65277412 Msa0695650:CDS 20.0%
!!! TTTTTTATTTCTAGTGAGAA+AGG - chr4_4:65277439-65277458 Msa0695650:CDS 20.0%
! GTCAAAACATAACATAATCA+TGG - chr4_4:65275670-65275689 Msa0695650:three_prime_UTR 25.0%
! GTTTCATCATGATAATCATA+TGG - chr4_4:65275859-65275878 Msa0695650:CDS 25.0%
!! ATGAGGAGAATAGAAATTTT+TGG - chr4_4:65275942-65275961 Msa0695650:CDS 25.0%
!! TGAGGAGAATAGAAATTTTT+GGG - chr4_4:65275943-65275962 Msa0695650:CDS 25.0%
! GTGCAATTTGTAATACTAAA+TGG - chr4_4:65276338-65276357 Msa0695650:intron 25.0%
! AAGGTCAAAATAGATCATAT+GGG + chr4_4:65276573-65276592 None:intergenic 25.0%
! TAAGGTCAAAATAGATCATA+TGG + chr4_4:65276574-65276593 None:intergenic 25.0%
! AATAATAGTGCAACAGAAAA+AGG + chr4_4:65276627-65276646 None:intergenic 25.0%
! ACAAGAATGTATTAGGTATT+AGG - chr4_4:65276670-65276689 Msa0695650:intron 25.0%
! CAAGAATGTATTAGGTATTA+GGG - chr4_4:65276671-65276690 Msa0695650:intron 25.0%
! AAGAATGTATTAGGTATTAG+GGG - chr4_4:65276672-65276691 Msa0695650:intron 25.0%
! ATGTGATTATAACTATACAG+GGG - chr4_4:65276926-65276945 Msa0695650:CDS 25.0%
! CATAATCAAGATTATGTGTA+TGG - chr4_4:65277124-65277143 Msa0695650:intron 25.0%
!! ATGTATTTATGTTTTCTCCA+TGG + chr4_4:65277191-65277210 None:intergenic 25.0%
! TTCAACTAATTCACTTTCAT+TGG - chr4_4:65277247-65277266 Msa0695650:CDS 25.0%
! TCAACTAATTCACTTTCATT+GGG - chr4_4:65277248-65277267 Msa0695650:CDS 25.0%
! GTCAAAACATAACATAATCA+TGG - chr4_4:65275670-65275689 Msa0695650:three_prime_UTR 25.0%
! GTTTCATCATGATAATCATA+TGG - chr4_4:65275859-65275878 Msa0695650:CDS 25.0%
!! ATGAGGAGAATAGAAATTTT+TGG - chr4_4:65275942-65275961 Msa0695650:CDS 25.0%
!! TGAGGAGAATAGAAATTTTT+GGG - chr4_4:65275943-65275962 Msa0695650:CDS 25.0%
! GTGCAATTTGTAATACTAAA+TGG - chr4_4:65276338-65276357 Msa0695650:intron 25.0%
! AAGGTCAAAATAGATCATAT+GGG + chr4_4:65276573-65276592 None:intergenic 25.0%
! TAAGGTCAAAATAGATCATA+TGG + chr4_4:65276574-65276593 None:intergenic 25.0%
! AATAATAGTGCAACAGAAAA+AGG + chr4_4:65276627-65276646 None:intergenic 25.0%
! ACAAGAATGTATTAGGTATT+AGG - chr4_4:65276670-65276689 Msa0695650:intron 25.0%
! CAAGAATGTATTAGGTATTA+GGG - chr4_4:65276671-65276690 Msa0695650:intron 25.0%
! AAGAATGTATTAGGTATTAG+GGG - chr4_4:65276672-65276691 Msa0695650:intron 25.0%
! ATGTGATTATAACTATACAG+GGG - chr4_4:65276926-65276945 Msa0695650:CDS 25.0%
! CATAATCAAGATTATGTGTA+TGG - chr4_4:65277124-65277143 Msa0695650:intron 25.0%
!! ATGTATTTATGTTTTCTCCA+TGG + chr4_4:65277191-65277210 None:intergenic 25.0%
! TTCAACTAATTCACTTTCAT+TGG - chr4_4:65277247-65277266 Msa0695650:CDS 25.0%
! TCAACTAATTCACTTTCATT+GGG - chr4_4:65277248-65277267 Msa0695650:CDS 25.0%
!! TTTTCTTGATGATTGTGATG+TGG - chr4_4:65275808-65275827 Msa0695650:exon 30.0%
! TTTCTTGATGATTGTGATGT+GGG - chr4_4:65275809-65275828 Msa0695650:exon 30.0%
TCATGATAATCATATGGAGT+TGG - chr4_4:65275865-65275884 Msa0695650:CDS 30.0%
TAAAGAAGGTTGTAACAATG+AGG - chr4_4:65275925-65275944 Msa0695650:CDS 30.0%
! TTGTGTTGTGTTGACAAAAT+TGG - chr4_4:65276115-65276134 Msa0695650:CDS 30.0%
GTTCTGTTGAAATAAGCATA+AGG - chr4_4:65276173-65276192 Msa0695650:CDS 30.0%
ACTTGTCTCATAAAACAGTT+TGG + chr4_4:65276286-65276305 None:intergenic 30.0%
GTGATTTAAAAGGGACTTTA+AGG + chr4_4:65276592-65276611 None:intergenic 30.0%
CTGTTGCACTATTATTAATC+AGG - chr4_4:65276631-65276650 Msa0695650:intron 30.0%
GTTTGTATTTGAAAAGCAAC+AGG - chr4_4:65276720-65276739 Msa0695650:intron 30.0%
CATAATACAAGCAAAGAGAA+AGG - chr4_4:65276972-65276991 Msa0695650:CDS 30.0%
CAAAGAGAAAGGAGATATTA+GGG - chr4_4:65276983-65277002 Msa0695650:CDS 30.0%
AAGGAAAACAAAATGCACAT+GGG - chr4_4:65277164-65277183 Msa0695650:intron 30.0%
ATAAATACATGCAAGCTAAG+TGG - chr4_4:65277201-65277220 Msa0695650:intron 30.0%
!! GTTGAAGTGTTCCTTTTAAA+TGG + chr4_4:65277233-65277252 None:intergenic 30.0%
!! TTTTCTTGATGATTGTGATG+TGG - chr4_4:65275808-65275827 Msa0695650:exon 30.0%
! TTTCTTGATGATTGTGATGT+GGG - chr4_4:65275809-65275828 Msa0695650:exon 30.0%
TCATGATAATCATATGGAGT+TGG - chr4_4:65275865-65275884 Msa0695650:CDS 30.0%
TAAAGAAGGTTGTAACAATG+AGG - chr4_4:65275925-65275944 Msa0695650:CDS 30.0%
! TTGTGTTGTGTTGACAAAAT+TGG - chr4_4:65276115-65276134 Msa0695650:CDS 30.0%
GTTCTGTTGAAATAAGCATA+AGG - chr4_4:65276173-65276192 Msa0695650:CDS 30.0%
ACTTGTCTCATAAAACAGTT+TGG + chr4_4:65276286-65276305 None:intergenic 30.0%
GTGATTTAAAAGGGACTTTA+AGG + chr4_4:65276592-65276611 None:intergenic 30.0%
CTGTTGCACTATTATTAATC+AGG - chr4_4:65276631-65276650 Msa0695650:intron 30.0%
GTTTGTATTTGAAAAGCAAC+AGG - chr4_4:65276720-65276739 Msa0695650:intron 30.0%
CATAATACAAGCAAAGAGAA+AGG - chr4_4:65276972-65276991 Msa0695650:CDS 30.0%
CAAAGAGAAAGGAGATATTA+GGG - chr4_4:65276983-65277002 Msa0695650:CDS 30.0%
AAGGAAAACAAAATGCACAT+GGG - chr4_4:65277164-65277183 Msa0695650:intron 30.0%
ATAAATACATGCAAGCTAAG+TGG - chr4_4:65277201-65277220 Msa0695650:intron 30.0%
!! GTTGAAGTGTTCCTTTTAAA+TGG + chr4_4:65277233-65277252 None:intergenic 30.0%
CAATATGCAGACACAAATCT+TGG - chr4_4:65275770-65275789 Msa0695650:three_prime_UTR 35.0%
GTTGGATATTGAAGATGATG+AGG - chr4_4:65275883-65275902 Msa0695650:CDS 35.0%
ATGAGGATAAATCTAGTGAG+AGG - chr4_4:65275900-65275919 Msa0695650:CDS 35.0%
TGAGGATAAATCTAGTGAGA+GGG - chr4_4:65275901-65275920 Msa0695650:CDS 35.0%
! CAACAGAACTTGTTCTGTAT+AGG + chr4_4:65276162-65276181 None:intergenic 35.0%
TGTAGTTGTAGTAGACAAAG+TGG - chr4_4:65276244-65276263 Msa0695650:intron 35.0%
!!! TAAAACCAGCATTTTGAAGC+TGG + chr4_4:65276315-65276334 None:intergenic 35.0%
AGTTCACATAATCTTCCATC+AGG - chr4_4:65276364-65276383 Msa0695650:intron 35.0%
! TACAGGGGAACATACATTTT+TGG - chr4_4:65276941-65276960 Msa0695650:CDS 35.0%
GCAAAGAGAAAGGAGATATT+AGG - chr4_4:65276982-65277001 Msa0695650:CDS 35.0%
! TTTCGAGCACAATTTGAGAT+GGG - chr4_4:65277023-65277042 Msa0695650:CDS 35.0%
! CTTTTCCTACATAAACCTCA+GGG + chr4_4:65277088-65277107 None:intergenic 35.0%
TTATGTGTATGGCAGCTAAA+AGG - chr4_4:65277135-65277154 Msa0695650:intron 35.0%
GAAGGAAAACAAAATGCACA+TGG - chr4_4:65277163-65277182 Msa0695650:intron 35.0%
AGTGGTTAGGTCCATTTAAA+AGG - chr4_4:65277219-65277238 Msa0695650:CDS 35.0%
ATAGCAGTACAATGCATATC+AGG + chr4_4:65277350-65277369 None:intergenic 35.0%
!! TATTTCTAGTGAGAAAGGTG+TGG - chr4_4:65277444-65277463 Msa0695650:CDS 35.0%
GAATTCAAAGCAGGTTCAAA+CGG + chr4_4:65277525-65277544 None:intergenic 35.0%
CAATATGCAGACACAAATCT+TGG - chr4_4:65275770-65275789 Msa0695650:three_prime_UTR 35.0%
GTTGGATATTGAAGATGATG+AGG - chr4_4:65275883-65275902 Msa0695650:CDS 35.0%
ATGAGGATAAATCTAGTGAG+AGG - chr4_4:65275900-65275919 Msa0695650:CDS 35.0%
TGAGGATAAATCTAGTGAGA+GGG - chr4_4:65275901-65275920 Msa0695650:CDS 35.0%
! CAACAGAACTTGTTCTGTAT+AGG + chr4_4:65276162-65276181 None:intergenic 35.0%
TGTAGTTGTAGTAGACAAAG+TGG - chr4_4:65276244-65276263 Msa0695650:intron 35.0%
!!! TAAAACCAGCATTTTGAAGC+TGG + chr4_4:65276315-65276334 None:intergenic 35.0%
AGTTCACATAATCTTCCATC+AGG - chr4_4:65276364-65276383 Msa0695650:intron 35.0%
! TACAGGGGAACATACATTTT+TGG - chr4_4:65276941-65276960 Msa0695650:CDS 35.0%
GCAAAGAGAAAGGAGATATT+AGG - chr4_4:65276982-65277001 Msa0695650:CDS 35.0%
! TTTCGAGCACAATTTGAGAT+GGG - chr4_4:65277023-65277042 Msa0695650:CDS 35.0%
! CTTTTCCTACATAAACCTCA+GGG + chr4_4:65277088-65277107 None:intergenic 35.0%
TTATGTGTATGGCAGCTAAA+AGG - chr4_4:65277135-65277154 Msa0695650:intron 35.0%
GAAGGAAAACAAAATGCACA+TGG - chr4_4:65277163-65277182 Msa0695650:intron 35.0%
AGTGGTTAGGTCCATTTAAA+AGG - chr4_4:65277219-65277238 Msa0695650:CDS 35.0%
ATAGCAGTACAATGCATATC+AGG + chr4_4:65277350-65277369 None:intergenic 35.0%
!! TATTTCTAGTGAGAAAGGTG+TGG - chr4_4:65277444-65277463 Msa0695650:CDS 35.0%
GAATTCAAAGCAGGTTCAAA+CGG + chr4_4:65277525-65277544 None:intergenic 35.0%
! GTTTTCGTAGGGAATTGTCT+AGG - chr4_4:65275735-65275754 Msa0695650:three_prime_UTR 40.0%
! GATGATTGTGATGTGGGTTT+TGG - chr4_4:65275815-65275834 Msa0695650:exon 40.0%
TCTAGTGAGAGGGTTAAAGA+AGG - chr4_4:65275911-65275930 Msa0695650:CDS 40.0%
TTACCTGCAAAAGCTGAAGT+TGG + chr4_4:65275973-65275992 None:intergenic 40.0%
TGCAACGAAAGAAGCAATTG+AGG - chr4_4:65276198-65276217 Msa0695650:CDS 40.0%
AAAACAGTTTGGACACGATC+TGG + chr4_4:65276275-65276294 None:intergenic 40.0%
TCTAACCAGCTTCAAAATGC+TGG - chr4_4:65276307-65276326 Msa0695650:intron 40.0%
CTTTCGAGCACAATTTGAGA+TGG - chr4_4:65277022-65277041 Msa0695650:CDS 40.0%
AGGATTATAACCGTCTTGTG+AGG - chr4_4:65277057-65277076 Msa0695650:intron 40.0%
AAACTCCCTGAGGTTTATGT+AGG - chr4_4:65277080-65277099 Msa0695650:intron 40.0%
! GCTTTTCCTACATAAACCTC+AGG + chr4_4:65277089-65277108 None:intergenic 40.0%
TACATGCAAGCTAAGTGGTT+AGG - chr4_4:65277206-65277225 Msa0695650:intron 40.0%
TAATCCAAAGCAGAAGCCAA+AGG - chr4_4:65277289-65277308 Msa0695650:CDS 40.0%
ACAGTTAACATCGATGCCTT+TGG + chr4_4:65277308-65277327 None:intergenic 40.0%
!! AAAGGCATCGATGTTAACTG+TGG - chr4_4:65277307-65277326 Msa0695650:CDS 40.0%
! GTTTTCGTAGGGAATTGTCT+AGG - chr4_4:65275735-65275754 Msa0695650:three_prime_UTR 40.0%
! GATGATTGTGATGTGGGTTT+TGG - chr4_4:65275815-65275834 Msa0695650:exon 40.0%
TCTAGTGAGAGGGTTAAAGA+AGG - chr4_4:65275911-65275930 Msa0695650:CDS 40.0%
TTACCTGCAAAAGCTGAAGT+TGG + chr4_4:65275973-65275992 None:intergenic 40.0%
TGCAACGAAAGAAGCAATTG+AGG - chr4_4:65276198-65276217 Msa0695650:CDS 40.0%
AAAACAGTTTGGACACGATC+TGG + chr4_4:65276275-65276294 None:intergenic 40.0%
TCTAACCAGCTTCAAAATGC+TGG - chr4_4:65276307-65276326 Msa0695650:intron 40.0%
CTTTCGAGCACAATTTGAGA+TGG - chr4_4:65277022-65277041 Msa0695650:CDS 40.0%
AGGATTATAACCGTCTTGTG+AGG - chr4_4:65277057-65277076 Msa0695650:intron 40.0%
AAACTCCCTGAGGTTTATGT+AGG - chr4_4:65277080-65277099 Msa0695650:intron 40.0%
! GCTTTTCCTACATAAACCTC+AGG + chr4_4:65277089-65277108 None:intergenic 40.0%
TACATGCAAGCTAAGTGGTT+AGG - chr4_4:65277206-65277225 Msa0695650:intron 40.0%
TAATCCAAAGCAGAAGCCAA+AGG - chr4_4:65277289-65277308 Msa0695650:CDS 40.0%
ACAGTTAACATCGATGCCTT+TGG + chr4_4:65277308-65277327 None:intergenic 40.0%
!! AAAGGCATCGATGTTAACTG+TGG - chr4_4:65277307-65277326 Msa0695650:CDS 40.0%
!!! ATTTGCCAGCTGGTTTTCGT+AGG - chr4_4:65275723-65275742 Msa0695650:three_prime_UTR 45.0%
!!! TTTGCCAGCTGGTTTTCGTA+GGG - chr4_4:65275724-65275743 Msa0695650:three_prime_UTR 45.0%
AATTCCCTACGAAAACCAGC+TGG + chr4_4:65275731-65275750 None:intergenic 45.0%
! CAACCAACTTCAGCTTTTGC+AGG - chr4_4:65275967-65275986 Msa0695650:CDS 45.0%
TGAGATGGGAAAAGCAAGTG+AGG - chr4_4:65277037-65277056 Msa0695650:intron 45.0%
!! GGCAGCTAAAAGGTGTTTGA+AGG - chr4_4:65277145-65277164 Msa0695650:intron 45.0%
ACAAAATGCACATGGGACCA+TGG - chr4_4:65277171-65277190 Msa0695650:intron 45.0%
!!! ATTTGCCAGCTGGTTTTCGT+AGG - chr4_4:65275723-65275742 Msa0695650:three_prime_UTR 45.0%
!!! TTTGCCAGCTGGTTTTCGTA+GGG - chr4_4:65275724-65275743 Msa0695650:three_prime_UTR 45.0%
AATTCCCTACGAAAACCAGC+TGG + chr4_4:65275731-65275750 None:intergenic 45.0%
! CAACCAACTTCAGCTTTTGC+AGG - chr4_4:65275967-65275986 Msa0695650:CDS 45.0%
TGAGATGGGAAAAGCAAGTG+AGG - chr4_4:65277037-65277056 Msa0695650:intron 45.0%
!! GGCAGCTAAAAGGTGTTTGA+AGG - chr4_4:65277145-65277164 Msa0695650:intron 45.0%
ACAAAATGCACATGGGACCA+TGG - chr4_4:65277171-65277190 Msa0695650:intron 45.0%
CCTAGTCACTCGTGCTAAAG+AGG + chr4_4:65275702-65275721 None:intergenic 50.0%
CCTCTTTAGCACGAGTGACT+AGG - chr4_4:65275699-65275718 Msa0695650:three_prime_UTR 50.0%
GTGACTAGGTATTTGCCAGC+TGG - chr4_4:65275713-65275732 Msa0695650:three_prime_UTR 50.0%
CAGGGAGTTTCCTCACAAGA+CGG + chr4_4:65277070-65277089 None:intergenic 50.0%
TCTTGTGAGGAAACTCCCTG+AGG - chr4_4:65277070-65277089 Msa0695650:intron 50.0%
! GATGCCTTTGGCTTCTGCTT+TGG + chr4_4:65277296-65277315 None:intergenic 50.0%
CCTAGTCACTCGTGCTAAAG+AGG + chr4_4:65275702-65275721 None:intergenic 50.0%
CCTCTTTAGCACGAGTGACT+AGG - chr4_4:65275699-65275718 Msa0695650:three_prime_UTR 50.0%
GTGACTAGGTATTTGCCAGC+TGG - chr4_4:65275713-65275732 Msa0695650:three_prime_UTR 50.0%
CAGGGAGTTTCCTCACAAGA+CGG + chr4_4:65277070-65277089 None:intergenic 50.0%
TCTTGTGAGGAAACTCCCTG+AGG - chr4_4:65277070-65277089 Msa0695650:intron 50.0%
! GATGCCTTTGGCTTCTGCTT+TGG + chr4_4:65277296-65277315 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 65275655 65277549 65275655 ID=Msa0695650;Name=Msa0695650
chr4_4 mRNA 65275655 65277549 65275655 ID=Msa0695650-mRNA-1;Parent=Msa0695650;Name=Msa0695650-mRNA-1;_AED=0.12;_eAED=0.12;_QI=34|1|1|1|1|1|3|167|322
chr4_4 exon 65275655 65276258 65275655 ID=Msa0695650-mRNA-1:exon:18793;Parent=Msa0695650-mRNA-1
chr4_4 exon 65276819 65277050 65276819 ID=Msa0695650-mRNA-1:exon:18792;Parent=Msa0695650-mRNA-1
chr4_4 exon 65277216 65277549 65277216 ID=Msa0695650-mRNA-1:exon:18791;Parent=Msa0695650-mRNA-1
chr4_4 five_prime_UTR 65277516 65277549 65277516 ID=Msa0695650-mRNA-1:five_prime_utr;Parent=Msa0695650-mRNA-1
chr4_4 CDS 65277216 65277515 65277216 ID=Msa0695650-mRNA-1:cds;Parent=Msa0695650-mRNA-1
chr4_4 CDS 65276819 65277050 65276819 ID=Msa0695650-mRNA-1:cds;Parent=Msa0695650-mRNA-1
chr4_4 CDS 65275822 65276258 65275822 ID=Msa0695650-mRNA-1:cds;Parent=Msa0695650-mRNA-1
chr4_4 three_prime_UTR 65275655 65275821 65275655 ID=Msa0695650-mRNA-1:three_prime_utr;Parent=Msa0695650-mRNA-1
Gene Sequence

>Msa0695650

ATGGGTGCAACCTCTTTAGCACGAGTGACTAGGTATTTGCCAGCTGGTTTTCGTAGGGAATTGTCTAGGCAAAAACTGCAGCAATATGCAGACACAAATCTTGGTGATGTTGAATTTGATTTTCTTGATGATTGTGATGTGGGTTTTGGTAGTAGTTCAAGTAGTGATGAGTTTCATCATGATAATCATATGGAGTTGGATATTGAAGATGATGAGGATAAATCTAGTGAGAGGGTTAAAGAAGGTTGTAACAATGAGGAGAATAGAAATTTTTGGGACAACCAACTTCAGCTTTTGCAGACAAACCTATACAGAACAAGTTCTGTTGAAATAAGCATAAGGAATGCAACGAAAGAAGCAATTGAGGAAATAGATAGATCAAAATTAGAGTGTAGTTGTAGTAGACAAAGTGGTGCAGCCAGATCGTGTCCAAACTGTTTTATGAGACAAGTCTCTAACCAGCTTCAAAATGCTGGTTTTAACAGTGCAATTTGTAATACTAAATGGACAAGTTCACATAATCTTCCATCAGGGGAACATACATTTTTGGATGTGATACATAATACAAGCAAAGAGAAAGGAGATATTAGGGTGATAATAGAACTAAACTTTCGAGCACAATTTGAGATGGGAAAAGCAAGTGAGGATTATAACCGTCTTGTGAGGAAACTCCCTGAGGTTTATGTAGGAAAAGCAGAGAGATTAAGCAACATAATCAAGATTATGTGTATGGCAGCTAAAAGGTGTTTGAAGGAAAACAAAATGCACATGGGACCATGGAGAAAACATAAATACATGCAAGCTAAGTGGTTAGGTCCATTTAAAAGGAACACTTCAACTAATTCACTTTCATTGGGATATTCTGAGAAGAATATTAATCCAAAGCAGAAGCCAAAGGCATCGATGTTAACTGTGGATTTGCTAGAGAAGTTTCCTGATATGCATTGTACTGCTATTGAAGTTGTGTAG
Protein sequence

>Msa0695650

MGATSLARVTRYLPAGFRRELSRQKLQQYADTNLGDVEFDFLDDCDVGFGSSSSSDEFHHDNHMELDIEDDEDKSSERVKEGCNNEENRNFWDNQLQLLQTNLYRTSSVEISIRNATKEAIEEIDRSKLECSCSRQSGAARSCPNCFMRQVSNQLQNAGFNSAICNTKWTSSHNLPSGEHTFLDVIHNTSKEKGDIRVIIELNFRAQFEMGKASEDYNRLVRKLPEVYVGKAERLSNIIKIMCMAAKRCLKENKMHMGPWRKHKYMQAKWLGPFKRNTSTNSLSLGYSEKNINPKQKPKASMLTVDLLEKFPDMHCTAIEVV*