Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0718780 | AES62131.1 | 77.632 | 76 | 10 | 1 | 6 | 74 | 1 | 76 | 6.51e-31 | 113 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0718780 | G7I518 | 77.632 | 76 | 10 | 1 | 6 | 74 | 1 | 76 | 3.11e-31 | 113 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0718780 | Msa0729800 | 0.801555 | 8.520071e-49 | -8.615850e-47 |
| Msa0718780 | Msa0743810 | 0.812073 | 5.093338e-51 | -8.615850e-47 |
| Msa0718780 | Msa0745060 | 0.818685 | 1.722602e-52 | -8.615850e-47 |
| Msa0718780 | Msa0825860 | 0.821876 | 3.197881e-53 | -8.615850e-47 |
| Msa0718780 | Msa0865320 | 0.822487 | 2.307644e-53 | -8.615850e-47 |
| Msa0718780 | Msa1025370 | 0.806076 | 9.802968e-50 | -8.615850e-47 |
| Msa0718780 | Msa1044830 | 0.819205 | 1.312115e-52 | -8.615850e-47 |
| Msa0718780 | Msa1100980 | 0.809644 | 1.708693e-50 | -8.615850e-47 |
| Msa0718780 | Msa1269670 | 0.818380 | 2.019921e-52 | -8.615850e-47 |
| Msa0718780 | Msa1270230 | 0.805143 | 1.538879e-49 | -8.615850e-47 |
| Msa0718780 | Msa1270270 | 0.812315 | 4.509484e-51 | -8.615850e-47 |
| Msa0718780 | Msa1287810 | 0.814838 | 1.256233e-51 | -8.615850e-47 |
| Msa0718780 | Msa1415310 | 0.838218 | 3.276600e-57 | -8.615850e-47 |
| Msa0414420 | Msa0718780 | 0.817045 | 4.042338e-52 | -8.615850e-47 |
| Msa0267000 | Msa0718780 | 0.806458 | 8.148116e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0718780 | MtrunA17_Chr1g0199591 | 77.632 | 76 | 10 | 1 | 6 | 74 | 1 | 76 | 5.98e-35 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 7 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAATGATTCACAAGAGAAT+TGG | 0.359166 | 5_1:+13119461 | Msa0718780:CDS |
| CGTTAACAAGCTGTTGTTGT+TGG | 0.410445 | 5_1:+13119591 | Msa0718780:CDS |
| TTATGAGTCTGTGATGTTCA+TGG | 0.412377 | 5_1:+13119618 | Msa0718780:CDS |
| ATTTGTTCAACACACGTATG+TGG | 0.525210 | 5_1:-13119516 | None:intergenic |
| TGTGATGTTCATGGTGATGA+TGG | 0.528435 | 5_1:+13119627 | Msa0718780:CDS |
| CTGCGTGTGAACAGAGAGGA+TGG | 0.615522 | 5_1:+13119650 | Msa0718780:CDS |
| ATGGCTGCGTGTGAACAGAG+AGG | 0.769554 | 5_1:+13119646 | Msa0718780:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAATTCATGAAAAATTATT+AGG | + | chr5_1:13119038-13119057 | Msa0718780:intron | 15.0% |
| !! | AAATTAAAAATTAAAGTTGC+TGG | - | chr5_1:13119332-13119351 | None:intergenic | 15.0% |
| !! | GAAATTCATGAAAAATTATT+AGG | + | chr5_1:13119038-13119057 | Msa0718780:intron | 15.0% |
| !! | AAATTAAAAATTAAAGTTGC+TGG | - | chr5_1:13119332-13119351 | None:intergenic | 15.0% |
| !!! | AAACTTGAAATGTTTTTGTA+TGG | - | chr5_1:13119281-13119300 | None:intergenic | 20.0% |
| !! | TTATGGGTTTAAAAAAGATT+GGG | + | chr5_1:13119541-13119560 | Msa0718780:intron | 20.0% |
| !!! | AAACTTGAAATGTTTTTGTA+TGG | - | chr5_1:13119281-13119300 | None:intergenic | 20.0% |
| !! | TTATGGGTTTAAAAAAGATT+GGG | + | chr5_1:13119541-13119560 | Msa0718780:intron | 20.0% |
| !!! | CATATTCTGATTCTAAAATC+CGG | + | chr5_1:13118975-13118994 | Msa0718780:intron | 25.0% |
| ! | GTTATGGGTTTAAAAAAGAT+TGG | + | chr5_1:13119540-13119559 | Msa0718780:intron | 25.0% |
| ! | TATGGGTTTAAAAAAGATTG+GGG | + | chr5_1:13119542-13119561 | Msa0718780:intron | 25.0% |
| !!! | CATATTCTGATTCTAAAATC+CGG | + | chr5_1:13118975-13118994 | Msa0718780:intron | 25.0% |
| ! | GTTATGGGTTTAAAAAAGAT+TGG | + | chr5_1:13119540-13119559 | Msa0718780:intron | 25.0% |
| ! | TATGGGTTTAAAAAAGATTG+GGG | + | chr5_1:13119542-13119561 | Msa0718780:intron | 25.0% |
| ! | TCTAAAATCCGGATAAATGA+TGG | + | chr5_1:13118986-13119005 | Msa0718780:intron | 30.0% |
| CTAAAATCCGGATAAATGAT+GGG | + | chr5_1:13118987-13119006 | Msa0718780:intron | 30.0% | |
| CTGTTGAATATTACATGATG+GGG | - | chr5_1:13119368-13119387 | None:intergenic | 30.0% | |
| GCTGTTGAATATTACATGAT+GGG | - | chr5_1:13119369-13119388 | None:intergenic | 30.0% | |
| AGCTGTTGAATATTACATGA+TGG | - | chr5_1:13119370-13119389 | None:intergenic | 30.0% | |
| TCAATGATTCACAAGAGAAT+TGG | + | chr5_1:13119461-13119480 | Msa0718780:CDS | 30.0% | |
| TGTGTTGAACAAATGGTTAT+GGG | + | chr5_1:13119525-13119544 | Msa0718780:intron | 30.0% | |
| TAAAAAAGATTGGGGTTTCA+AGG | + | chr5_1:13119550-13119569 | Msa0718780:intron | 30.0% | |
| ! | TCTAAAATCCGGATAAATGA+TGG | + | chr5_1:13118986-13119005 | Msa0718780:intron | 30.0% |
| CTAAAATCCGGATAAATGAT+GGG | + | chr5_1:13118987-13119006 | Msa0718780:intron | 30.0% | |
| CTGTTGAATATTACATGATG+GGG | - | chr5_1:13119368-13119387 | None:intergenic | 30.0% | |
| GCTGTTGAATATTACATGAT+GGG | - | chr5_1:13119369-13119388 | None:intergenic | 30.0% | |
| AGCTGTTGAATATTACATGA+TGG | - | chr5_1:13119370-13119389 | None:intergenic | 30.0% | |
| TCAATGATTCACAAGAGAAT+TGG | + | chr5_1:13119461-13119480 | Msa0718780:CDS | 30.0% | |
| TGTGTTGAACAAATGGTTAT+GGG | + | chr5_1:13119525-13119544 | Msa0718780:intron | 30.0% | |
| TAAAAAAGATTGGGGTTTCA+AGG | + | chr5_1:13119550-13119569 | Msa0718780:intron | 30.0% | |
| GTGAGATTTAGTATGGGAAA+GGG | + | chr5_1:13119102-13119121 | Msa0718780:intron | 35.0% | |
| AAGATCAACTTTCCCATCAT+GGG | - | chr5_1:13119137-13119156 | None:intergenic | 35.0% | |
| TAAGATCAACTTTCCCATCA+TGG | - | chr5_1:13119138-13119157 | None:intergenic | 35.0% | |
| TTGGAGGAGAGAATATTGTT+GGG | - | chr5_1:13119230-13119249 | None:intergenic | 35.0% | |
| ! | TTAAAGTTGCTGGATTTGCT+TGG | - | chr5_1:13119322-13119341 | None:intergenic | 35.0% |
| ATTTGTTCAACACACGTATG+TGG | - | chr5_1:13119519-13119538 | None:intergenic | 35.0% | |
| ACATACGTGTGTTGAACAAA+TGG | + | chr5_1:13119518-13119537 | Msa0718780:intron | 35.0% | |
| GTGTGTTGAACAAATGGTTA+TGG | + | chr5_1:13119524-13119543 | Msa0718780:intron | 35.0% | |
| TTATGAGTCTGTGATGTTCA+TGG | + | chr5_1:13119618-13119637 | Msa0718780:CDS | 35.0% | |
| GTGAGATTTAGTATGGGAAA+GGG | + | chr5_1:13119102-13119121 | Msa0718780:intron | 35.0% | |
| AAGATCAACTTTCCCATCAT+GGG | - | chr5_1:13119137-13119156 | None:intergenic | 35.0% | |
| TAAGATCAACTTTCCCATCA+TGG | - | chr5_1:13119138-13119157 | None:intergenic | 35.0% | |
| TTGGAGGAGAGAATATTGTT+GGG | - | chr5_1:13119230-13119249 | None:intergenic | 35.0% | |
| ! | TTAAAGTTGCTGGATTTGCT+TGG | - | chr5_1:13119322-13119341 | None:intergenic | 35.0% |
| ATTTGTTCAACACACGTATG+TGG | - | chr5_1:13119519-13119538 | None:intergenic | 35.0% | |
| ACATACGTGTGTTGAACAAA+TGG | + | chr5_1:13119518-13119537 | Msa0718780:intron | 35.0% | |
| GTGTGTTGAACAAATGGTTA+TGG | + | chr5_1:13119524-13119543 | Msa0718780:intron | 35.0% | |
| TTATGAGTCTGTGATGTTCA+TGG | + | chr5_1:13119618-13119637 | Msa0718780:CDS | 35.0% | |
| TCAGACACCCATCATTTATC+CGG | - | chr5_1:13118997-13119016 | None:intergenic | 40.0% | |
| CTCCTAATACCTCTAAACCA+CGG | + | chr5_1:13119080-13119099 | Msa0718780:intron | 40.0% | |
| TAAATCTCACCGTGGTTTAG+AGG | - | chr5_1:13119092-13119111 | None:intergenic | 40.0% | |
| AACCACGGTGAGATTTAGTA+TGG | + | chr5_1:13119095-13119114 | Msa0718780:intron | 40.0% | |
| ACCACGGTGAGATTTAGTAT+GGG | + | chr5_1:13119096-13119115 | Msa0718780:intron | 40.0% | |
| GGTGAGATTTAGTATGGGAA+AGG | + | chr5_1:13119101-13119120 | Msa0718780:intron | 40.0% | |
| TGGAGGAGAGAATATTGTTG+GGG | - | chr5_1:13119229-13119248 | None:intergenic | 40.0% | |
| GTTGGAGGAGAGAATATTGT+TGG | - | chr5_1:13119231-13119250 | None:intergenic | 40.0% | |
| CGTTAACAAGCTGTTGTTGT+TGG | + | chr5_1:13119591-13119610 | Msa0718780:CDS | 40.0% | |
| ! | TGTGATGTTCATGGTGATGA+TGG | + | chr5_1:13119627-13119646 | Msa0718780:CDS | 40.0% |
| ! | TGGAAGCAAATGCTTTTGTG+TGG | + | chr5_1:13119670-13119689 | Msa0718780:CDS | 40.0% |
| ! | AAGCAAATGCTTTTGTGTGG+TGG | + | chr5_1:13119673-13119692 | Msa0718780:CDS | 40.0% |
| TCAGACACCCATCATTTATC+CGG | - | chr5_1:13118997-13119016 | None:intergenic | 40.0% | |
| CTCCTAATACCTCTAAACCA+CGG | + | chr5_1:13119080-13119099 | Msa0718780:intron | 40.0% | |
| TAAATCTCACCGTGGTTTAG+AGG | - | chr5_1:13119092-13119111 | None:intergenic | 40.0% | |
| AACCACGGTGAGATTTAGTA+TGG | + | chr5_1:13119095-13119114 | Msa0718780:intron | 40.0% | |
| ACCACGGTGAGATTTAGTAT+GGG | + | chr5_1:13119096-13119115 | Msa0718780:intron | 40.0% | |
| GGTGAGATTTAGTATGGGAA+AGG | + | chr5_1:13119101-13119120 | Msa0718780:intron | 40.0% | |
| TGGAGGAGAGAATATTGTTG+GGG | - | chr5_1:13119229-13119248 | None:intergenic | 40.0% | |
| GTTGGAGGAGAGAATATTGT+TGG | - | chr5_1:13119231-13119250 | None:intergenic | 40.0% | |
| CGTTAACAAGCTGTTGTTGT+TGG | + | chr5_1:13119591-13119610 | Msa0718780:CDS | 40.0% | |
| ! | TGTGATGTTCATGGTGATGA+TGG | + | chr5_1:13119627-13119646 | Msa0718780:CDS | 40.0% |
| ! | TGGAAGCAAATGCTTTTGTG+TGG | + | chr5_1:13119670-13119689 | Msa0718780:CDS | 40.0% |
| ! | AAGCAAATGCTTTTGTGTGG+TGG | + | chr5_1:13119673-13119692 | Msa0718780:CDS | 40.0% |
| ! | TGATGGGTGTCTGAGAAATG+TGG | + | chr5_1:13119003-13119022 | Msa0718780:intron | 45.0% |
| !! | CACCGTGGTTTAGAGGTATT+AGG | - | chr5_1:13119085-13119104 | None:intergenic | 45.0% |
| TCCCATACTAAATCTCACCG+TGG | - | chr5_1:13119100-13119119 | None:intergenic | 45.0% | |
| AGGGAAGTAGTTTCCCATGA+TGG | + | chr5_1:13119121-13119140 | Msa0718780:intron | 45.0% | |
| GGGAAGTAGTTTCCCATGAT+GGG | + | chr5_1:13119122-13119141 | Msa0718780:intron | 45.0% | |
| ! | AAGTTGCTGGATTTGCTTGG+AGG | - | chr5_1:13119319-13119338 | None:intergenic | 45.0% |
| !! | ATGCTTTTGTGTGGTGGTGA+TGG | + | chr5_1:13119679-13119698 | Msa0718780:CDS | 45.0% |
| !! | TGCTTTTGTGTGGTGGTGAT+GGG | + | chr5_1:13119680-13119699 | Msa0718780:CDS | 45.0% |
| ! | TGATGGGTGTCTGAGAAATG+TGG | + | chr5_1:13119003-13119022 | Msa0718780:intron | 45.0% |
| !! | CACCGTGGTTTAGAGGTATT+AGG | - | chr5_1:13119085-13119104 | None:intergenic | 45.0% |
| TCCCATACTAAATCTCACCG+TGG | - | chr5_1:13119100-13119119 | None:intergenic | 45.0% | |
| AGGGAAGTAGTTTCCCATGA+TGG | + | chr5_1:13119121-13119140 | Msa0718780:intron | 45.0% | |
| GGGAAGTAGTTTCCCATGAT+GGG | + | chr5_1:13119122-13119141 | Msa0718780:intron | 45.0% | |
| ! | AAGTTGCTGGATTTGCTTGG+AGG | - | chr5_1:13119319-13119338 | None:intergenic | 45.0% |
| !! | ATGCTTTTGTGTGGTGGTGA+TGG | + | chr5_1:13119679-13119698 | Msa0718780:CDS | 45.0% |
| !! | TGCTTTTGTGTGGTGGTGAT+GGG | + | chr5_1:13119680-13119699 | Msa0718780:CDS | 45.0% |
| TCCTCCAACCACAACCTGCA+AGG | + | chr5_1:13119242-13119261 | Msa0718780:intron | 55.0% | |
| AGTTGCAGCCTTGCAGGTTG+TGG | - | chr5_1:13119253-13119272 | None:intergenic | 55.0% | |
| GAGAAGAGTTGCAGCCTTGC+AGG | - | chr5_1:13119259-13119278 | None:intergenic | 55.0% | |
| ATGGCTGCGTGTGAACAGAG+AGG | + | chr5_1:13119646-13119665 | Msa0718780:CDS | 55.0% | |
| CTGCGTGTGAACAGAGAGGA+TGG | + | chr5_1:13119650-13119669 | Msa0718780:CDS | 55.0% | |
| TCCTCCAACCACAACCTGCA+AGG | + | chr5_1:13119242-13119261 | Msa0718780:intron | 55.0% | |
| AGTTGCAGCCTTGCAGGTTG+TGG | - | chr5_1:13119253-13119272 | None:intergenic | 55.0% | |
| GAGAAGAGTTGCAGCCTTGC+AGG | - | chr5_1:13119259-13119278 | None:intergenic | 55.0% | |
| ATGGCTGCGTGTGAACAGAG+AGG | + | chr5_1:13119646-13119665 | Msa0718780:CDS | 55.0% | |
| CTGCGTGTGAACAGAGAGGA+TGG | + | chr5_1:13119650-13119669 | Msa0718780:CDS | 55.0% | |
| GCCTTGCAGGTTGTGGTTGG+AGG | - | chr5_1:13119246-13119265 | None:intergenic | 60.0% | |
| GCAGCCTTGCAGGTTGTGGT+TGG | - | chr5_1:13119249-13119268 | None:intergenic | 60.0% | |
| GCCTTGCAGGTTGTGGTTGG+AGG | - | chr5_1:13119246-13119265 | None:intergenic | 60.0% | |
| GCAGCCTTGCAGGTTGTGGT+TGG | - | chr5_1:13119249-13119268 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 13118949 | 13119705 | 13118949 | ID=Msa0718780;Name=Msa0718780 |
| chr5_1 | mRNA | 13118949 | 13119705 | 13118949 | ID=Msa0718780-mRNA-1;Parent=Msa0718780;Name=Msa0718780-mRNA-1;_AED=0.11;_eAED=0.13;_QI=0|0|0|0.66|0|0|3|0|74 |
| chr5_1 | exon | 13118949 | 13118953 | 13118949 | ID=Msa0718780-mRNA-1:exon:5399;Parent=Msa0718780-mRNA-1 |
| chr5_1 | exon | 13119454 | 13119525 | 13119454 | ID=Msa0718780-mRNA-1:exon:5400;Parent=Msa0718780-mRNA-1 |
| chr5_1 | exon | 13119558 | 13119705 | 13119558 | ID=Msa0718780-mRNA-1:exon:5401;Parent=Msa0718780-mRNA-1 |
| chr5_1 | CDS | 13118949 | 13118953 | 13118949 | ID=Msa0718780-mRNA-1:cds;Parent=Msa0718780-mRNA-1 |
| chr5_1 | CDS | 13119454 | 13119525 | 13119454 | ID=Msa0718780-mRNA-1:cds;Parent=Msa0718780-mRNA-1 |
| chr5_1 | CDS | 13119558 | 13119705 | 13119558 | ID=Msa0718780-mRNA-1:cds;Parent=Msa0718780-mRNA-1 |
| Gene Sequence |
| Protein sequence |
