AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1415310


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1415310 MtrunA17_Chr1g0171561 72.549 51 4 1 18 58 361 411 6.07e-15 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCATAATACTGCTCCCCTT+TGG 0.399303 tig0024199:+60488 None:intergenic
GGCAAATTGTAGGGTGTGAA+AGG 0.482250 tig0024199:-60516 Msa1415310:intron
CGAAGAGATGGCCGTACAGT+TGG 0.497848 tig0024199:-60408 Msa1415310:CDS
AGCAGTATACTGCAGGTCTT+CGG 0.516574 tig0024199:-60431 Msa1415310:CDS
AATAATGAAACAGCCGAAAG+AGG 0.517338 tig0024199:-60735 None:intergenic
GTGTGAAAGGAGAAGCCAAA+GGG 0.524133 tig0024199:-60503 Msa1415310:CDS
GCAGGTCTTCGGCGAAGAGA+TGG 0.543464 tig0024199:-60420 Msa1415310:CDS
GCTCAATCTGTCCACGCCAT+CGG 0.552859 tig0024199:+60688 None:intergenic
GCTGAGAAGCAGTATACTGC+AGG 0.565896 tig0024199:-60438 Msa1415310:CDS
CAGTATACTGCTTCTCAGCA+CGG 0.579616 tig0024199:+60442 None:intergenic
AAAAGATGGCGGAGGAGGAG+TGG 0.584037 tig0024199:-60711 Msa1415310:CDS
GGCGGAGGAGGAGTGGCCGA+TGG 0.593201 tig0024199:-60704 Msa1415310:CDS
AAAGACGTAATCCAACTGTA+CGG 0.652711 tig0024199:+60397 None:intergenic
TGTGAAAGGAGAAGCCAAAG+GGG 0.666256 tig0024199:-60502 Msa1415310:CDS
GGTGTGAAAGGAGAAGCCAA+AGG 0.691576 tig0024199:-60504 Msa1415310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTTAGTTGTTCTTGAATGAC+TGG - tig0024199:60522-60541 Msa1415310:intron 30.0%
CAGACTCAAAATGTGATAGT+AGG + tig0024199:60571-60590 None:intergenic 35.0%
!! ACTATCACATTTTGAGTCTG+AGG - tig0024199:60571-60590 Msa1415310:intron 35.0%
AAAGACGTAATCCAACTGTA+CGG + tig0024199:60732-60751 None:intergenic 35.0%
ATATGACATAGCAGTGAGTC+TGG + tig0024199:60470-60489 None:intergenic 40.0%
AACCTCCTCATGTTATTGTC+GGG + tig0024199:60501-60520 None:intergenic 40.0%
CAACCTCCTCATGTTATTGT+CGG + tig0024199:60502-60521 None:intergenic 40.0%
CAGCCGAAAGAGGAAAAAGA+TGG - tig0024199:60401-60420 Msa1415310:CDS 45.0%
! CCGCCATCTTTTTCCTCTTT+CGG + tig0024199:60407-60426 None:intergenic 45.0%
CCGAAAGAGGAAAAAGATGG+CGG - tig0024199:60404-60423 Msa1415310:CDS 45.0%
! AAAGAGGAAAAAGATGGCGG+AGG - tig0024199:60407-60426 Msa1415310:CDS 45.0%
TCGTGCCTATGGCAAATTGT+AGG - tig0024199:60600-60619 Msa1415310:intron 45.0%
CGTGCCTATGGCAAATTGTA+GGG - tig0024199:60601-60620 Msa1415310:intron 45.0%
CACACCCTACAATTTGCCAT+AGG + tig0024199:60608-60627 None:intergenic 45.0%
GGCAAATTGTAGGGTGTGAA+AGG - tig0024199:60610-60629 Msa1415310:intron 45.0%
GTGTGAAAGGAGAAGCCAAA+GGG - tig0024199:60623-60642 Msa1415310:intron 45.0%
TGTGAAAGGAGAAGCCAAAG+GGG - tig0024199:60624-60643 Msa1415310:intron 45.0%
GTCATAATACTGCTCCCCTT+TGG + tig0024199:60641-60660 None:intergenic 45.0%
CAGTATACTGCTTCTCAGCA+CGG + tig0024199:60687-60706 None:intergenic 45.0%
AGCAGTATACTGCAGGTCTT+CGG - tig0024199:60695-60714 Msa1415310:intron 45.0%
GTCCTCCCGACAATAACATG+AGG - tig0024199:60493-60512 Msa1415310:CDS 50.0%
CTCCTCATGTTATTGTCGGG+AGG + tig0024199:60498-60517 None:intergenic 50.0%
CTCCCGACAATAACATGAGG+AGG - tig0024199:60496-60515 Msa1415310:CDS 50.0%
TGAGGTTGTACTCGTGCCTA+TGG - tig0024199:60589-60608 Msa1415310:intron 50.0%
GGTGTGAAAGGAGAAGCCAA+AGG - tig0024199:60622-60641 Msa1415310:intron 50.0%
GCTGAGAAGCAGTATACTGC+AGG - tig0024199:60688-60707 Msa1415310:intron 50.0%
!! GAGGAAAAAGATGGCGGAGG+AGG - tig0024199:60410-60429 Msa1415310:CDS 55.0%
!! AAAAGATGGCGGAGGAGGAG+TGG - tig0024199:60415-60434 Msa1415310:CDS 55.0%
GCTCAATCTGTCCACGCCAT+CGG + tig0024199:60441-60460 None:intergenic 55.0%
CGAAGAGATGGCCGTACAGT+TGG - tig0024199:60718-60737 Msa1415310:CDS 55.0%
GCAGGTCTTCGGCGAAGAGA+TGG - tig0024199:60706-60725 Msa1415310:CDS 60.0%
AGGAGGAGTGGCCGATGGCG+TGG - tig0024199:60427-60446 Msa1415310:CDS 70.0%
GGCGGAGGAGGAGTGGCCGA+TGG - tig0024199:60422-60441 Msa1415310:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0024199 gene 60395 60753 60395 ID=Msa1415310;Name=Msa1415310
tig0024199 mRNA 60395 60753 60395 ID=Msa1415310-mRNA-1;Parent=Msa1415310;Name=Msa1415310-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0|0|0.5|1|1|2|0|60
tig0024199 exon 60703 60753 60703 ID=Msa1415310-mRNA-1:exon:22328;Parent=Msa1415310-mRNA-1
tig0024199 exon 60395 60526 60395 ID=Msa1415310-mRNA-1:exon:22327;Parent=Msa1415310-mRNA-1
tig0024199 CDS 60703 60753 60703 ID=Msa1415310-mRNA-1:cds;Parent=Msa1415310-mRNA-1
tig0024199 CDS 60395 60526 60395 ID=Msa1415310-mRNA-1:cds;Parent=Msa1415310-mRNA-1
Gene Sequence

>Msa1415310

ATGAAACAGCCGAAAGAGGAAAAAGATGGCGGAGGAGGAGTGGCCGATGGCGGTGTGAAAGGAGAAGCCAAAGGGGAGCAGTATTATGACAATCAAAATGACAACAACTTACTCCGTGCTGAGAAGCAGTATACTGCAGGTCTTCGGCGAAGAGATGGCCGTACAGTTGGATTACGTCTTTAA

Protein sequence

>Msa1415310

MKQPKEEKDGGGGVADGGVKGEAKGEQYYDNQNDNNLLRAEKQYTAGLRRRDGRTVGLRL*