AlfalfaGEDB | Msa0274440

M. sativa cultivar zhongmu-4 / Msa0274440

KEGG: K10999
GO: GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005975 GO:0005976 GO:0006073 GO:0006950 GO:0006952 GO:0006970 GO:0007049 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009059 GO:0009250 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009832 GO:0009833 GO:0009834 GO:0009987 GO:0010035 GO:0012505 GO:0016020 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0016759 GO:0016760 GO:0022402 GO:0030243 GO:0030244 GO:0030312 GO:0033692 GO:0034637 GO:0034645 GO:0035251 GO:0042221 GO:0042546 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0050832 GO:0050896 GO:0051273 GO:0051274 GO:0051301 GO:0051704 GO:0051707 GO:0061640 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0098542 GO:1901576 GO:1901700 GO:1903047
PFAM: PF14569.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0274440	XP_013462596.1	99.387	979	4	1	1	977	1	979	0.0	2019

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0274440	sp\|Q8LPK5\|CESA8_ARATH	82.132	985	168	3	1	977	1	985	0.0	1674

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0274440	A0A072V4N8	99.387	979	4	1	1	977	1	979	0.0	2019

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0040660	Msa0274440	0.863425	2.512091e-64	-8.615850e-47
Msa0081250	Msa0274440	0.814085	1.844084e-51	-8.615850e-47
Msa0102040	Msa0274440	0.818235	2.178511e-52	-8.615850e-47
Msa0173780	Msa0274440	0.807653	4.550236e-50	-8.615850e-47
Msa0184680	Msa0274440	0.836856	7.322752e-57	-8.615850e-47
Msa0193850	Msa0274440	0.924979	3.410778e-90	-8.615850e-47
Msa0199960	Msa0274440	0.832015	1.203218e-55	-8.615850e-47
Msa0235710	Msa0274440	0.956397	3.246452e-114	-8.615850e-47
Msa0243600	Msa0274440	0.857668	1.395546e-62	-8.615850e-47
Msa0274440	Msa0280690	0.808300	3.314515e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0274440	MtrunA17_Chr2g0284211	99.387	979	4	1	1	977	1	979	2019
Msa0274440	MtrunA17_Chr8g0379241	81.864	998	153	8	1	977	1	991	1652
Msa0274440	MtrunA17_Chr2g0296991	65.098	1020	293	14	3	970	31	1039	1325
Msa0274440	MtrunA17_Chr4g0073711	63.221	1006	303	8	9	963	37	1026	1311
Msa0274440	MtrunA17_Chr3g0088821	66.631	929	280	12	75	977	154	1078	1263
Msa0274440	MtrunA17_Chr3g0137951	66.810	931	281	13	70	977	162	1087	1261
Msa0274440	MtrunA17_Chr8g0383701	67.010	873	259	8	110	962	220	1083	1243
Msa0274440	MtrunA17_Chr8g0352231	67.861	893	263	11	101	977	194	1078	1233
Msa0274440	MtrunA17_Chr1g0201731	67.281	868	262	6	110	964	228	1086	1233
Msa0274440	MtrunA17_Chr1g0183381	66.994	815	250	5	158	970	102	899	1169

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0274440	AT4G18780.1	82.132	985	168	3	1	977	1	985	1674
Msa0274440	AT5G44030.1	63.163	1037	304	13	9	977	23	1049	1320
Msa0274440	AT5G17420.1	63.101	1019	297	11	7	974	35	1025	1311
Msa0274440	AT5G44030.2	61.568	1059	314	10	9	977	23	1078	1311
Msa0274440	AT5G05170.1	61.392	1049	318	14	9	974	20	1064	1292
Msa0274440	AT2G25540.1	61.667	1020	318	15	9	961	33	1046	1289
Msa0274440	AT4G32410.1	61.725	1032	310	13	9	960	39	1065	1286
Msa0274440	AT5G64740.1	57.696	1059	344	12	9	977	39	1083	1253
Msa0274440	AT2G21770.1	66.104	888	268	10	110	977	212	1086	1235
Msa0274440	AT5G09870.1	64.892	883	280	9	110	977	201	1068	1224

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Find 243 sgRNAs with CRISPR-Local

Find 369 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGATCGGAAAGATTGAT+TGG	0.491570	2_3:-6265791	None:intergenic
AAAGACAGCATGGGGAAAAG+AGG	0.541039	2_3:+6265547	Msa0274440:CDS
AAAGAGGACAATGTCTACTA+AGG	0.520358	2_3:-6265856	None:intergenic
AAAGGACTTGACGGCATTCA+AGG	0.471693	2_3:+6265052	Msa0274440:CDS
AAAGTACAATAAGCAACGAG+GGG	0.696943	2_3:-6265962	None:intergenic
AAAGTCAGCTGTCTCCACAA+GGG	0.704045	2_3:-6263721	None:intergenic
AAATAGAGTGGAAAGTTGGA+AGG	0.558941	2_3:+6262624	Msa0274440:CDS
AACCTTGTTGGTGTTGTAGC+TGG	0.415993	2_3:+6266573	Msa0274440:CDS
AACGAGCAGTTCTGGGTAAT+TGG	0.298572	2_3:+6266378	Msa0274440:CDS
AACTCTTGTGGTGAACAAGT+TGG	0.583291	2_3:+6261578	Msa0274440:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAGTATAGTTTGAGAAGTTA+AGG	+	chr2_3:6261487-6261506	Msa0274440:five_prime_UTR	30.0%
TTCACCAAACTTTGATATCT+GGG	-	chr2_3:6262217-6262236	None:intergenic	30.0%
GTAGAATTAAATGATGAGTC+TGG	+	chr2_3:6262586-6262605	Msa0274440:intron	30.0%
ACAGAACTGTTATAATCATG+CGG	+	chr2_3:6263162-6263181	Msa0274440:CDS	30.0%
TATTGATAGGAGACCATTTA+GGG	-	chr2_3:6263313-6263332	None:intergenic	30.0%
TAAATGGTCTCCTATCAATA+GGG	+	chr2_3:6263313-6263332	Msa0274440:CDS	30.0%
AAACTTTATATTGCCATGTC+AGG	+	chr2_3:6263511-6263530	Msa0274440:intron	30.0%
TCATGTTATGTTTCAGATGA+TGG	+	chr2_3:6263675-6263694	Msa0274440:CDS	30.0%
TCAGAAGATTGACTATTTGA+AGG	+	chr2_3:6263812-6263831	Msa0274440:CDS	30.0%
AATACTGAATGTCAGAAACA+AGG	-	chr2_3:6264065-6264084	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	6261254	6267220	6261254	ID=Msa0274440;Name=Msa0274440
chr2_3	mRNA	6261254	6267220	6261254	ID=Msa0274440-mRNA-1;Parent=Msa0274440;Name=Msa0274440-mRNA-1;_AED=0.09;_eAED=0.09;_QI=297\|1\|1\|1\|1\|1\|13\|363\|977
chr2_3	exon	6261254	6261728	6261254	ID=Msa0274440-mRNA-1:exon:2907;Parent=Msa0274440-mRNA-1
chr2_3	exon	6262035	6262114	6262035	ID=Msa0274440-mRNA-1:exon:2908;Parent=Msa0274440-mRNA-1
chr2_3	exon	6262444	6262495	6262444	ID=Msa0274440-mRNA-1:exon:2909;Parent=Msa0274440-mRNA-1
chr2_3	exon	6262590	6262737	6262590	ID=Msa0274440-mRNA-1:exon:2910;Parent=Msa0274440-mRNA-1
chr2_3	exon	6263088	6263363	6263088	ID=Msa0274440-mRNA-1:exon:2911;Parent=Msa0274440-mRNA-1
chr2_3	exon	6263533	6263878	6263533	ID=Msa0274440-mRNA-1:exon:2912;Parent=Msa0274440-mRNA-1
chr2_3	exon	6264124	6264261	6264124	ID=Msa0274440-mRNA-1:exon:2913;Parent=Msa0274440-mRNA-1
chr2_3	exon	6264493	6264618	6264493	ID=Msa0274440-mRNA-1:exon:2914;Parent=Msa0274440-mRNA-1
chr2_3	exon	6264705	6264917	6264705	ID=Msa0274440-mRNA-1:exon:2915;Parent=Msa0274440-mRNA-1
chr2_3	exon	6265043	6265277	6265043	ID=Msa0274440-mRNA-1:exon:2916;Parent=Msa0274440-mRNA-1
chr2_3	exon	6265369	6265568	6265369	ID=Msa0274440-mRNA-1:exon:2917;Parent=Msa0274440-mRNA-1
chr2_3	exon	6265674	6266027	6265674	ID=Msa0274440-mRNA-1:exon:2918;Parent=Msa0274440-mRNA-1
chr2_3	exon	6266270	6267220	6266270	ID=Msa0274440-mRNA-1:exon:2919;Parent=Msa0274440-mRNA-1
chr2_3	five_prime_UTR	6261254	6261550	6261254	ID=Msa0274440-mRNA-1:five_prime_utr;Parent=Msa0274440-mRNA-1
chr2_3	CDS	6261551	6261728	6261551	ID=Msa0274440-mRNA-1:cds;Parent=Msa0274440-mRNA-1
chr2_3	CDS	6262035	6262114	6262035	ID=Msa0274440-mRNA-1:cds;Parent=Msa0274440-mRNA-1
chr2_3	CDS	6262444	6262495	6262444	ID=Msa0274440-mRNA-1:cds;Parent=Msa0274440-mRNA-1
chr2_3	CDS	6262590	6262737	6262590	ID=Msa0274440-mRNA-1:cds;Parent=Msa0274440-mRNA-1
chr2_3	CDS	6263088	6263363	6263088	ID=Msa0274440-mRNA-1:cds;Parent=Msa0274440-mRNA-1
chr2_3	CDS	6263533	6263878	6263533	ID=Msa0274440-mRNA-1:cds;Parent=Msa0274440-mRNA-1
chr2_3	CDS	6264124	6264261	6264124	ID=Msa0274440-mRNA-1:cds;Parent=Msa0274440-mRNA-1
chr2_3	CDS	6264493	6264618	6264493	ID=Msa0274440-mRNA-1:cds;Parent=Msa0274440-mRNA-1
chr2_3	CDS	6264705	6264917	6264705	ID=Msa0274440-mRNA-1:cds;Parent=Msa0274440-mRNA-1
chr2_3	CDS	6265043	6265277	6265043	ID=Msa0274440-mRNA-1:cds;Parent=Msa0274440-mRNA-1
chr2_3	CDS	6265369	6265568	6265369	ID=Msa0274440-mRNA-1:cds;Parent=Msa0274440-mRNA-1
chr2_3	CDS	6265674	6266027	6265674	ID=Msa0274440-mRNA-1:cds;Parent=Msa0274440-mRNA-1
chr2_3	CDS	6266270	6266857	6266270	ID=Msa0274440-mRNA-1:cds;Parent=Msa0274440-mRNA-1
chr2_3	three_prime_UTR	6266858	6267220	6266858	ID=Msa0274440-mRNA-1:three_prime_utr;Parent=Msa0274440-mRNA-1

Gene Sequence

>Msa0274440

ATGATGGAATCTGGAGTTAGCTTTTGTAACTCTTGTGGTGAACAAGTTGGAGTTGATGCTAATGGAGAGGTGTTTGTGGCTTGTCATGAGTGTTATTTCCCAATTTGTAAGGCTTGTGTTGATTATGAAACCAGTGAGGGACGTAGTGTTTGTTTGAGATGTGGCAATCCCTATGCTGATAGAACAAGAGGTAAGGATGACACCAAAGTTCCTGCAAATCAATCCACAACAACGGCTGCACAGATCAGCACTTCTCAGGATGTTGGACTCCATGCTAGGCATATCAGTACAGTGTCCACAGTGGACAGTGAATTAAATGATGAGTCTGGGAATCCTATCTGGAAAAATAGAGTGGAAAGTTGGAAGGAGAAGGACAAAAAGAACAAGAAGAAAAAGGAAGCACCTAAGGCTGGAAATGAGACTCCAGTTCCACATGAACAGCAGATGGATGAAATGCAGCCCTCCTCTGAGGCTGCAGCTGCTGAGCCACTTTCAGTGATAATTCCAGTCTCAAAATCGAAACTTGGACCATACAGAACTGTTATAATCATGCGGCTCGTAATATTGGGTCTGTTTTTCCATTATAGAGTTACACATCCAGTTGATAGTGCTTTTGGTCTGTGGTTGACATCTATCATCTGTGAGATCTGGTTTGCTTTTTCCTGGGTGTTAGATCAGTTCCCTAAATGGTCTCCTATCAATAGGGTTGCTTTTACGGACAGGCTCTCGGCAAGGTTTGAAAGAGAGGGTGAACCTTGTCAGCTTGCTCCTGTGGATTTCTTTGTCAGTACAGTTGATCCGTTGAAAGAACCGCCGTTGATCACAGCTAACACAGTTCTTTCTATCCTTGCCGTGGACTATCCTGTGGAAAAAGTATCATGTTATGTTTCAGATGATGGTGCTGCAATGCTGACATTTGAATCCCTTGTGGAGACAGCTGACTTTGCAAGAAAGTGGGTTCCATTTTGCAAAAAGTTTGAGATTGAACCACGTGCACCTGAGTTTTACTTCTCTCAGAAGATTGACTATTTGAAGGACAAAGTTCAACCTTCTTTTGTGAAGGAACGTAGAGCAATGAAAAGAGAATATGAAGAGTATAAAGTCAGAGTTAATGCTTTGGTAGCTAAGGCTCAGAAAACACCAGATGAAGGGTGGACTATGCAAGATGGAACCTCTTGGCCAGGAAATAATTCACGCGACCATCCTGGCATGATTCAGGTTTTCCTTGGACACAGTGGTGCTCACGACATAGAAGGAAACGAACTTCCAAGGTTGGTCTATGTTTCCAGAGAGAAAAGGCCAGGTTACCAACACCACAAAAAGGCTGGTGCTGAAAATGCACTGGTGAGGGTGTCTGCAGTTCTCACAAACGCTCCCTACATTCTCAATCTTGACTGTGATCATTATGTTAACAATAGCAAGGCTGTTCGAGAAGCGATGTGTTTTCTGATGGATCCAGTAGTCGGTAGAGATGTTTGTTATGTGCAGTTCCCCCAAAGATTTGATGGTATTGATCGTAGTGATCGATATGCCAACCGGAACACAGTTTTCTTCGATGTTAATATGAAAGGACTTGACGGCATTCAAGGACCTGTATATGTGGGGACTGGATGTGTTTTCAATCGACAAGCGCTTTATGGATACAGTCCACCTTCTATGCCCCCATTACCAAAATCTTCATCTTGCTGTTGCTTCCGCTCAAAGAAGCCTGCCAAAGATGTCTCAGAGCTTTATAAAGATGCAAAGAGGGAAGAGCTTGATGCTGCTATTTTTAATCTCAGGGAGATTGAGAACTATGATGAGTATGAGAGGTCAATGCTTATTTCACAACTTAGCTTTGAAAAAACATTTGGTTTGTCCACTGTTTTCATTGAATCTACACTAATGGAGAATGGTGGTGTGTCTGAATCTGCGGATCCCTCAATGCTGATCAAGGAGGCCATTCATGTAATCGGTTGTGGCTACGAAGAAAAGACAGCATGGGGAAAAGAGATTGGTTGGATATATGGTTCAGTGACTGAGGATATCTTAACGGGGTTCAAGATGCAATGTCGTGGATGGAGGTCAATTTACTGCATGCCCTTAAGGCCTGCGTTCAAAGGGTCAGCGCCAATCAATCTTTCCGATCGTTTGCACCAAGTTCTTCGATGGGCTCTTGGATCAGTTGAAATTTTCCTTAGTAGACATTGTCCTCTTTGGTATGGATTTGCTGGTGGTCGTCTGAAACTGCTGCAGAGACTGGCTTATATAAACACCATCGTTTACCCTTTCACATCCCTTCCCCTCGTTGCTTATTGTACTTTGCCTGCAATTTGCCTACTCACAGGAAAATTCATCATACCAACGCTATCAAACATTGCAAGTGCACTTTTTCTTGGACTTTTCATCTCTATTATATTGACCAGTGTTCTTGAGCTGAGGTGGAGCGGTGTTACCATAGAAGATTTATGGCGTAACGAGCAGTTCTGGGTAATTGGAGGTGTTTCGGCTCATCTTTTTGCTGTCTTCCAAGGATTCCTAAAGATGTTAGCAGGTGTTGACACAAACTTCACTGTGACAGCAAAAGCTGCTGATGATGCAGAATTTGGTGAACTATATATGATCAAATGGACAACACTCTTAATTCCTCCAACAAGTCTTATCATTATTAACCTTGTTGGTGTTGTAGCTGGATTTTCTGATGCACTTAATGGTGGATATGAATCTTGGGGACCACTCATTGGAAAAGTTTTCTTCGCATTTTGGGTGATTTTTCATCTTTATCCATTCCTTAAGGGTCTTATGGGTCGCCAAAACCGTACTCCAACCATTGTTATTCTTTGGTCAGTGCTTTTGGCTTCTGTCTTCTCACTTGTTTGGGTTAAGATTAATCCATTTGTGAGCAAAGTTGATAGTTCAGCTATTTCTCAGACTTGTATTTCTATTGATTGTTAA

Protein sequence

>Msa0274440

MMESGVSFCNSCGEQVGVDANGEVFVACHECYFPICKACVDYETSEGRSVCLRCGNPYADRTRGKDDTKVPANQSTTTAAQISTSQDVGLHARHISTVSTVDSELNDESGNPIWKNRVESWKEKDKKNKKKKEAPKAGNETPVPHEQQMDEMQPSSEAAAAEPLSVIIPVSKSKLGPYRTVIIMRLVILGLFFHYRVTHPVDSAFGLWLTSIICEIWFAFSWVLDQFPKWSPINRVAFTDRLSARFEREGEPCQLAPVDFFVSTVDPLKEPPLITANTVLSILAVDYPVEKVSCYVSDDGAAMLTFESLVETADFARKWVPFCKKFEIEPRAPEFYFSQKIDYLKDKVQPSFVKERRAMKREYEEYKVRVNALVAKAQKTPDEGWTMQDGTSWPGNNSRDHPGMIQVFLGHSGAHDIEGNELPRLVYVSREKRPGYQHHKKAGAENALVRVSAVLTNAPYILNLDCDHYVNNSKAVREAMCFLMDPVVGRDVCYVQFPQRFDGIDRSDRYANRNTVFFDVNMKGLDGIQGPVYVGTGCVFNRQALYGYSPPSMPPLPKSSSCCCFRSKKPAKDVSELYKDAKREELDAAIFNLREIENYDEYERSMLISQLSFEKTFGLSTVFIESTLMENGGVSESADPSMLIKEAIHVIGCGYEEKTAWGKEIGWIYGSVTEDILTGFKMQCRGWRSIYCMPLRPAFKGSAPINLSDRLHQVLRWALGSVEIFLSRHCPLWYGFAGGRLKLLQRLAYINTIVYPFTSLPLVAYCTLPAICLLTGKFIIPTLSNIASALFLGLFISIILTSVLELRWSGVTIEDLWRNEQFWVIGGVSAHLFAVFQGFLKMLAGVDTNFTVTAKAADDAEFGELYMIKWTTLLIPPTSLIIINLVGVVAGFSDALNGGYESWGPLIGKVFFAFWVIFHLYPFLKGLMGRQNRTPTIVILWSVLLASVFSLVWVKINPFVSKVDSSAISQTCISIDC*