AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0733630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0733630 MtrunA17_Chr4g0020281 100.000 48 0 0 1 48 1 48 5.59e-29 97.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0733630 AT3G52040.1 72.917 48 13 0 1 48 1 48 1.37e-17 69.3

Find 49 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATATCCAACAAAATTTA+AGG 0.122688 5_1:+46965599 None:intergenic
GTTCTATGAATCTCAGAATT+TGG 0.189259 5_1:+46966110 None:intergenic
GAAATGAAAGAATTAGAATT+AGG 0.190429 5_1:+46967287 None:intergenic
TCCTCCCGTTGTAGTGTTAA+TGG 0.192394 5_1:+46966189 None:intergenic
TGCATAGAGGGTTGGTTTAA+TGG 0.192936 5_1:-46966058 Msa0733630:three_prime_UTR
AAAGCTAAGAAATGTGTTTA+TGG 0.202424 5_1:-46965830 Msa0733630:three_prime_UTR
TAATCAAGTGGTTGCCAAAA+AGG 0.245471 5_1:+46965926 None:intergenic
AATACTAATTATAGAGAAAT+AGG 0.269817 5_1:-46966152 Msa0733630:three_prime_UTR
GGAATAAAGAGTTAATTTAA+TGG 0.289905 5_1:+46965621 None:intergenic
AAATGAAAGAATTAGAATTA+GGG 0.311904 5_1:+46967288 None:intergenic
GCATTGATGAAAAGCGTTAT+TGG 0.317728 5_1:-46965558 Msa0733630:three_prime_UTR
AAGAATTAGAATTAGGGGTT+AGG 0.333926 5_1:+46967294 None:intergenic
GAATTGATTTCTTCTTAGTT+TGG 0.339268 5_1:+46967217 None:intergenic
AACGAAAGTTACAAAGGATA+TGG 0.347960 5_1:-46967067 Msa0733630:CDS
TCGATTTCGAAATATACAAT+TGG 0.376395 5_1:+46967334 None:intergenic
GAGGGTTGGTTTAATGGCAT+TGG 0.381497 5_1:-46966052 Msa0733630:three_prime_UTR
AATTGTCACATGGGAGTAGA+TGG 0.393050 5_1:-46965884 Msa0733630:three_prime_UTR
GTATATCCAACAAAATTTAA+GGG 0.401580 5_1:+46965600 None:intergenic
AGAATTAGAATTAGGGGTTA+GGG 0.415001 5_1:+46967295 None:intergenic
AATTTACCTTTGCGAGTTTG+AGG 0.415610 5_1:+46967171 None:intergenic
CGATTTCGAAATATACAATT+GGG 0.415895 5_1:+46967335 None:intergenic
ATGTGTTTATGGACTGTTAC+GGG 0.416741 5_1:-46965819 Msa0733630:three_prime_UTR
TGGTCATGATATTTGATGAT+TGG 0.457422 5_1:-46965768 Msa0733630:three_prime_UTR
ATATCCTTTGTAACTTTCGT+TGG 0.460250 5_1:+46967069 None:intergenic
GAAGCCATTAACACTACAAC+GGG 0.464801 5_1:-46966193 Msa0733630:three_prime_UTR
ACTTTGCCATGACGATTAGC+AGG 0.529511 5_1:+46967195 None:intergenic
TTTGAGGACACCATGCATAG+AGG 0.545592 5_1:-46966071 Msa0733630:three_prime_UTR
TTGAGGACACCATGCATAGA+GGG 0.546985 5_1:-46966070 Msa0733630:three_prime_UTR
AGGATCAGTTGAACATCTGT+CGG 0.555154 5_1:+46965983 None:intergenic
GGACACCATGCATAGAGGGT+TGG 0.560118 5_1:-46966066 Msa0733630:three_prime_UTR
TCAATTCCTGCTAATCGTCA+TGG 0.560841 5_1:-46967201 Msa0733630:CDS
GGATCAGTTGAACATCTGTC+GGG 0.563586 5_1:+46965984 None:intergenic
AATGTGTTTATGGACTGTTA+CGG 0.567882 5_1:-46965820 Msa0733630:three_prime_UTR
GTAACTTGTAAGCACTTGTA+CGG 0.571883 5_1:-46966218 Msa0733630:exon
AATGAAAGAATTAGAATTAG+GGG 0.573583 5_1:+46967289 None:intergenic
AGATTCATAGAACGAAGCTA+AGG 0.585176 5_1:-46966100 Msa0733630:three_prime_UTR
GATTCATAGAACGAAGCTAA+GGG 0.611997 5_1:-46966099 Msa0733630:three_prime_UTR
TTAAACCAACCCTCTATGCA+TGG 0.616170 5_1:+46966061 None:intergenic
AAAGTACCTCAAACTCGCAA+AGG 0.619602 5_1:-46967177 Msa0733630:intron
GCCATTAACACTACAACGGG+AGG 0.622819 5_1:-46966190 Msa0733630:three_prime_UTR
ACTTGTAAGCACTTGTACGG+TGG 0.626055 5_1:-46966215 Msa0733630:three_prime_UTR
AGATGTTCAACTGATCCTCT+CGG 0.627027 5_1:-46965978 Msa0733630:three_prime_UTR
TATCTGCAGAAGAAGACTGA+GGG 0.633770 5_1:-46966010 Msa0733630:three_prime_UTR
AAGCAAGGAGAATTGTCACA+TGG 0.643229 5_1:-46965894 Msa0733630:three_prime_UTR
AGCAAGGAGAATTGTCACAT+GGG 0.662171 5_1:-46965893 Msa0733630:three_prime_UTR
ATGGCATTGGATCAGTATCG+AGG 0.665786 5_1:-46966039 Msa0733630:three_prime_UTR
GGAAGCCATTAACACTACAA+CGG 0.667610 5_1:-46966194 Msa0733630:three_prime_UTR
GAAGCCAACGAAAGTTACAA+AGG 0.673022 5_1:-46967073 Msa0733630:CDS
ATATCTGCAGAAGAAGACTG+AGG 0.682318 5_1:-46966011 Msa0733630:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAACAAAAAAATAAATAA+TGG + chr5_1:46965745-46965764 None:intergenic 10.0%
!!! ATAATTATCTAATTTGAAAT+TGG - chr5_1:46966502-46966521 Msa0733630:intron 10.0%
!! AACAACAAAAAAATAAATAA+TGG + chr5_1:46965745-46965764 None:intergenic 10.0%
!!! ATAATTATCTAATTTGAAAT+TGG - chr5_1:46966502-46966521 Msa0733630:intron 10.0%
!! AAATGAAAGAATTAGAATTA+GGG + chr5_1:46965599-46965618 None:intergenic 15.0%
!!! TTTAATTTTCAGTTATGATT+TGG - chr5_1:46965915-46965934 Msa0733630:three_prime_UTR 15.0%
!!! TTAATTTTCAGTTATGATTT+GGG - chr5_1:46965916-46965935 Msa0733630:three_prime_UTR 15.0%
!! TATATTAACAGTTTAAACAA+TGG - chr5_1:46966592-46966611 Msa0733630:intron 15.0%
!!! TTCTCTATAATTAGTATTTT+TGG + chr5_1:46966730-46966749 None:intergenic 15.0%
!! AATACTAATTATAGAGAAAT+AGG - chr5_1:46966732-46966751 Msa0733630:intron 15.0%
!!! TATCATAACATTTTAAATTG+TGG - chr5_1:46967096-46967115 Msa0733630:intron 15.0%
!! AAATGAAAGAATTAGAATTA+GGG + chr5_1:46965599-46965618 None:intergenic 15.0%
!!! TTTAATTTTCAGTTATGATT+TGG - chr5_1:46965915-46965934 Msa0733630:three_prime_UTR 15.0%
!!! TTAATTTTCAGTTATGATTT+GGG - chr5_1:46965916-46965935 Msa0733630:three_prime_UTR 15.0%
!! TATATTAACAGTTTAAACAA+TGG - chr5_1:46966592-46966611 Msa0733630:intron 15.0%
!!! TTCTCTATAATTAGTATTTT+TGG + chr5_1:46966730-46966749 None:intergenic 15.0%
!! AATACTAATTATAGAGAAAT+AGG - chr5_1:46966732-46966751 Msa0733630:intron 15.0%
!!! TATCATAACATTTTAAATTG+TGG - chr5_1:46967096-46967115 Msa0733630:intron 15.0%
!! AATGAAAGAATTAGAATTAG+GGG + chr5_1:46965598-46965617 None:intergenic 20.0%
!! GAAATGAAAGAATTAGAATT+AGG + chr5_1:46965600-46965619 None:intergenic 20.0%
!!! TCTAATTTGAAATTGGTTTT+TGG - chr5_1:46966509-46966528 Msa0733630:intron 20.0%
!!! CTAATTTGAAATTGGTTTTT+GGG - chr5_1:46966510-46966529 Msa0733630:intron 20.0%
!! CTGTTAATATACATTATCAT+AGG + chr5_1:46966583-46966602 None:intergenic 20.0%
!!! ATATTGACTTAACTCTTTTT+TGG - chr5_1:46966625-46966644 Msa0733630:intron 20.0%
!!! TATTGACTTAACTCTTTTTT+GGG - chr5_1:46966626-46966645 Msa0733630:intron 20.0%
!!! ATTGTGTAAATGATTTTTCA+CGG + chr5_1:46966932-46966951 None:intergenic 20.0%
!!! CTTGAATATTTTTAATCAAG+TGG + chr5_1:46966973-46966992 None:intergenic 20.0%
!! TGATTAAAAATATTCAAGCA+AGG - chr5_1:46966975-46966994 Msa0733630:intron 20.0%
!!! TATGAAGTATGAACTTTTTA+AGG + chr5_1:46967243-46967262 None:intergenic 20.0%
!! GGAATAAAGAGTTAATTTAA+TGG + chr5_1:46967266-46967285 None:intergenic 20.0%
!!! TTTATTCCCTTAAATTTTGT+TGG - chr5_1:46967278-46967297 Msa0733630:five_prime_UTR 20.0%
!! GTATATCCAACAAAATTTAA+GGG + chr5_1:46967287-46967306 None:intergenic 20.0%
!! TGTATATCCAACAAAATTTA+AGG + chr5_1:46967288-46967307 None:intergenic 20.0%
!!! TAAATTTTGTTGGATATACA+AGG - chr5_1:46967288-46967307 Msa0733630:five_prime_UTR 20.0%
!! AATGAAAGAATTAGAATTAG+GGG + chr5_1:46965598-46965617 None:intergenic 20.0%
!! GAAATGAAAGAATTAGAATT+AGG + chr5_1:46965600-46965619 None:intergenic 20.0%
!!! TCTAATTTGAAATTGGTTTT+TGG - chr5_1:46966509-46966528 Msa0733630:intron 20.0%
!!! CTAATTTGAAATTGGTTTTT+GGG - chr5_1:46966510-46966529 Msa0733630:intron 20.0%
!! CTGTTAATATACATTATCAT+AGG + chr5_1:46966583-46966602 None:intergenic 20.0%
!!! ATATTGACTTAACTCTTTTT+TGG - chr5_1:46966625-46966644 Msa0733630:intron 20.0%
!!! TATTGACTTAACTCTTTTTT+GGG - chr5_1:46966626-46966645 Msa0733630:intron 20.0%
!!! ATTGTGTAAATGATTTTTCA+CGG + chr5_1:46966932-46966951 None:intergenic 20.0%
!!! CTTGAATATTTTTAATCAAG+TGG + chr5_1:46966973-46966992 None:intergenic 20.0%
!! TGATTAAAAATATTCAAGCA+AGG - chr5_1:46966975-46966994 Msa0733630:intron 20.0%
!!! TATGAAGTATGAACTTTTTA+AGG + chr5_1:46967243-46967262 None:intergenic 20.0%
!! GGAATAAAGAGTTAATTTAA+TGG + chr5_1:46967266-46967285 None:intergenic 20.0%
!!! TTTATTCCCTTAAATTTTGT+TGG - chr5_1:46967278-46967297 Msa0733630:five_prime_UTR 20.0%
!! GTATATCCAACAAAATTTAA+GGG + chr5_1:46967287-46967306 None:intergenic 20.0%
!! TGTATATCCAACAAAATTTA+AGG + chr5_1:46967288-46967307 None:intergenic 20.0%
!!! TAAATTTTGTTGGATATACA+AGG - chr5_1:46967288-46967307 Msa0733630:five_prime_UTR 20.0%
! CGATTTCGAAATATACAATT+GGG + chr5_1:46965552-46965571 None:intergenic 25.0%
! TCGATTTCGAAATATACAAT+TGG + chr5_1:46965553-46965572 None:intergenic 25.0%
!! CAGAAATCAAAACTTTTCAA+GGG - chr5_1:46965644-46965663 Msa0733630:three_prime_UTR 25.0%
!! GAATTGATTTCTTCTTAGTT+TGG + chr5_1:46965670-46965689 None:intergenic 25.0%
!! ACAATTTTCTGATCTTTACA+GGG - chr5_1:46965777-46965796 Msa0733630:three_prime_UTR 25.0%
! GAAAATTAGAGAAAAGCAAA+AGG + chr5_1:46965861-46965880 None:intergenic 25.0%
!!! AATTTGTAGTGTGTTTTTTG+TGG - chr5_1:46965887-46965906 Msa0733630:three_prime_UTR 25.0%
! TATCTTATAGGACTTGATTA+GGG + chr5_1:46966205-46966224 None:intergenic 25.0%
! TTATCTTATAGGACTTGATT+AGG + chr5_1:46966206-46966225 None:intergenic 25.0%
! TTGATAAGCACTTATCTTAT+AGG + chr5_1:46966217-46966236 None:intergenic 25.0%
!!! CATACTTTCTTGTTTTACTT+TGG + chr5_1:46966263-46966282 None:intergenic 25.0%
! CAAAGTAAAACAAGAAAGTA+TGG - chr5_1:46966261-46966280 Msa0733630:intron 25.0%
!! AAGCTATAAACTGTTTTCAT+CGG - chr5_1:46966301-46966320 Msa0733630:intron 25.0%
!!! ATTGACTTAACTCTTTTTTG+GGG - chr5_1:46966627-46966646 Msa0733630:intron 25.0%
! AAAGCTAAGAAATGTGTTTA+TGG - chr5_1:46967054-46967073 Msa0733630:intron 25.0%
! CGATTTCGAAATATACAATT+GGG + chr5_1:46965552-46965571 None:intergenic 25.0%
! TCGATTTCGAAATATACAAT+TGG + chr5_1:46965553-46965572 None:intergenic 25.0%
!! CAGAAATCAAAACTTTTCAA+GGG - chr5_1:46965644-46965663 Msa0733630:three_prime_UTR 25.0%
!! GAATTGATTTCTTCTTAGTT+TGG + chr5_1:46965670-46965689 None:intergenic 25.0%
!! ACAATTTTCTGATCTTTACA+GGG - chr5_1:46965777-46965796 Msa0733630:three_prime_UTR 25.0%
! GAAAATTAGAGAAAAGCAAA+AGG + chr5_1:46965861-46965880 None:intergenic 25.0%
!!! AATTTGTAGTGTGTTTTTTG+TGG - chr5_1:46965887-46965906 Msa0733630:three_prime_UTR 25.0%
! TATCTTATAGGACTTGATTA+GGG + chr5_1:46966205-46966224 None:intergenic 25.0%
! TTATCTTATAGGACTTGATT+AGG + chr5_1:46966206-46966225 None:intergenic 25.0%
! TTGATAAGCACTTATCTTAT+AGG + chr5_1:46966217-46966236 None:intergenic 25.0%
!!! CATACTTTCTTGTTTTACTT+TGG + chr5_1:46966263-46966282 None:intergenic 25.0%
! CAAAGTAAAACAAGAAAGTA+TGG - chr5_1:46966261-46966280 Msa0733630:intron 25.0%
!! AAGCTATAAACTGTTTTCAT+CGG - chr5_1:46966301-46966320 Msa0733630:intron 25.0%
!!! ATTGACTTAACTCTTTTTTG+GGG - chr5_1:46966627-46966646 Msa0733630:intron 25.0%
! AAAGCTAAGAAATGTGTTTA+TGG - chr5_1:46967054-46967073 Msa0733630:intron 25.0%
AGAATTAGAATTAGGGGTTA+GGG + chr5_1:46965592-46965611 None:intergenic 30.0%
AAGAATTAGAATTAGGGGTT+AGG + chr5_1:46965593-46965612 None:intergenic 30.0%
! GCAGAAATCAAAACTTTTCA+AGG - chr5_1:46965643-46965662 Msa0733630:three_prime_UTR 30.0%
! CACAATTTTCTGATCTTTAC+AGG - chr5_1:46965776-46965795 Msa0733630:three_prime_UTR 30.0%
ATATCCTTTGTAACTTTCGT+TGG + chr5_1:46965818-46965837 None:intergenic 30.0%
AACGAAAGTTACAAAGGATA+TGG - chr5_1:46965817-46965836 Msa0733630:three_prime_UTR 30.0%
!! TTCAGTTATGATTTGGGTTT+TGG - chr5_1:46965922-46965941 Msa0733630:three_prime_UTR 30.0%
! AAAGTATTCATGTTTCTGCT+TGG - chr5_1:46965962-46965981 Msa0733630:three_prime_UTR 30.0%
TTTCATAAGCTCAATCCAAA+CGG + chr5_1:46966005-46966024 None:intergenic 30.0%
TTATGGCAGTAAATAACCTT+AGG - chr5_1:46966421-46966440 Msa0733630:intron 30.0%
GCTCATGAATAATTTACCTA+AGG + chr5_1:46966440-46966459 None:intergenic 30.0%
AACAGTTTAAACAATGGTTG+AGG - chr5_1:46966598-46966617 Msa0733630:intron 30.0%
GTTCTATGAATCTCAGAATT+TGG + chr5_1:46966777-46966796 None:intergenic 30.0%
! AATGTGTTTATGGACTGTTA+CGG - chr5_1:46967064-46967083 Msa0733630:CDS 30.0%
! TGGTCATGATATTTGATGAT+TGG - chr5_1:46967116-46967135 Msa0733630:intron 30.0%
AGAATTAGAATTAGGGGTTA+GGG + chr5_1:46965592-46965611 None:intergenic 30.0%
AAGAATTAGAATTAGGGGTT+AGG + chr5_1:46965593-46965612 None:intergenic 30.0%
! GCAGAAATCAAAACTTTTCA+AGG - chr5_1:46965643-46965662 Msa0733630:three_prime_UTR 30.0%
! CACAATTTTCTGATCTTTAC+AGG - chr5_1:46965776-46965795 Msa0733630:three_prime_UTR 30.0%
ATATCCTTTGTAACTTTCGT+TGG + chr5_1:46965818-46965837 None:intergenic 30.0%
AACGAAAGTTACAAAGGATA+TGG - chr5_1:46965817-46965836 Msa0733630:three_prime_UTR 30.0%
!! TTCAGTTATGATTTGGGTTT+TGG - chr5_1:46965922-46965941 Msa0733630:three_prime_UTR 30.0%
! AAAGTATTCATGTTTCTGCT+TGG - chr5_1:46965962-46965981 Msa0733630:three_prime_UTR 30.0%
TTTCATAAGCTCAATCCAAA+CGG + chr5_1:46966005-46966024 None:intergenic 30.0%
TTATGGCAGTAAATAACCTT+AGG - chr5_1:46966421-46966440 Msa0733630:intron 30.0%
GCTCATGAATAATTTACCTA+AGG + chr5_1:46966440-46966459 None:intergenic 30.0%
AACAGTTTAAACAATGGTTG+AGG - chr5_1:46966598-46966617 Msa0733630:intron 30.0%
GTTCTATGAATCTCAGAATT+TGG + chr5_1:46966777-46966796 None:intergenic 30.0%
! AATGTGTTTATGGACTGTTA+CGG - chr5_1:46967064-46967083 Msa0733630:CDS 30.0%
! TGGTCATGATATTTGATGAT+TGG - chr5_1:46967116-46967135 Msa0733630:intron 30.0%
AATTTACCTTTGCGAGTTTG+AGG + chr5_1:46965716-46965735 None:intergenic 35.0%
CTGCTTGGATAAACAGATTA+AGG - chr5_1:46965977-46965996 Msa0733630:three_prime_UTR 35.0%
! TGATTAGGGTCTTTTTGGAT+TGG + chr5_1:46966191-46966210 None:intergenic 35.0%
AAGACACTAGCAAGAGTTTA+TGG - chr5_1:46966404-46966423 Msa0733630:intron 35.0%
ACAAGCTTCTGCATTTGAAT+CGG + chr5_1:46966462-46966481 None:intergenic 35.0%
!!! CTTTTTTGGGGCTTATCTTA+TGG - chr5_1:46966639-46966658 Msa0733630:intron 35.0%
! GTAACTTGTAAGCACTTGTA+CGG - chr5_1:46966666-46966685 Msa0733630:intron 35.0%
AGATTCATAGAACGAAGCTA+AGG - chr5_1:46966784-46966803 Msa0733630:intron 35.0%
GATTCATAGAACGAAGCTAA+GGG - chr5_1:46966785-46966804 Msa0733630:intron 35.0%
AATCAAGTGGTTGCCAAAAA+GGG + chr5_1:46966960-46966979 None:intergenic 35.0%
TAATCAAGTGGTTGCCAAAA+AGG + chr5_1:46966961-46966980 None:intergenic 35.0%
! ATGTGTTTATGGACTGTTAC+GGG - chr5_1:46967065-46967084 Msa0733630:CDS 35.0%
!! GCATTGATGAAAAGCGTTAT+TGG - chr5_1:46967326-46967345 Msa0733630:five_prime_UTR 35.0%
AATTTACCTTTGCGAGTTTG+AGG + chr5_1:46965716-46965735 None:intergenic 35.0%
CTGCTTGGATAAACAGATTA+AGG - chr5_1:46965977-46965996 Msa0733630:three_prime_UTR 35.0%
! TGATTAGGGTCTTTTTGGAT+TGG + chr5_1:46966191-46966210 None:intergenic 35.0%
AAGACACTAGCAAGAGTTTA+TGG - chr5_1:46966404-46966423 Msa0733630:intron 35.0%
ACAAGCTTCTGCATTTGAAT+CGG + chr5_1:46966462-46966481 None:intergenic 35.0%
!!! CTTTTTTGGGGCTTATCTTA+TGG - chr5_1:46966639-46966658 Msa0733630:intron 35.0%
! GTAACTTGTAAGCACTTGTA+CGG - chr5_1:46966666-46966685 Msa0733630:intron 35.0%
AGATTCATAGAACGAAGCTA+AGG - chr5_1:46966784-46966803 Msa0733630:intron 35.0%
GATTCATAGAACGAAGCTAA+GGG - chr5_1:46966785-46966804 Msa0733630:intron 35.0%
AATCAAGTGGTTGCCAAAAA+GGG + chr5_1:46966960-46966979 None:intergenic 35.0%
TAATCAAGTGGTTGCCAAAA+AGG + chr5_1:46966961-46966980 None:intergenic 35.0%
! ATGTGTTTATGGACTGTTAC+GGG - chr5_1:46967065-46967084 Msa0733630:CDS 35.0%
!! GCATTGATGAAAAGCGTTAT+TGG - chr5_1:46967326-46967345 Msa0733630:five_prime_UTR 35.0%
TCAATTCCTGCTAATCGTCA+TGG - chr5_1:46965683-46965702 Msa0733630:three_prime_UTR 40.0%
! AAAGTACCTCAAACTCGCAA+AGG - chr5_1:46965707-46965726 Msa0733630:three_prime_UTR 40.0%
GAAGCCAACGAAAGTTACAA+AGG - chr5_1:46965811-46965830 Msa0733630:three_prime_UTR 40.0%
AAACAGATTAAGGCTCCGTT+TGG - chr5_1:46965987-46966006 Msa0733630:three_prime_UTR 40.0%
! GGACTTGATTAGGGTCTTTT+TGG + chr5_1:46966196-46966215 None:intergenic 40.0%
GGAAGCCATTAACACTACAA+CGG - chr5_1:46966690-46966709 Msa0733630:intron 40.0%
GAAGCCATTAACACTACAAC+GGG - chr5_1:46966691-46966710 Msa0733630:intron 40.0%
!! GAAGCTAAGGGTTGATTTTG+AGG - chr5_1:46966797-46966816 Msa0733630:intron 40.0%
TTAAACCAACCCTCTATGCA+TGG + chr5_1:46966826-46966845 None:intergenic 40.0%
!! TGCATAGAGGGTTGGTTTAA+TGG - chr5_1:46966826-46966845 Msa0733630:intron 40.0%
ATATCTGCAGAAGAAGACTG+AGG - chr5_1:46966873-46966892 Msa0733630:intron 40.0%
TATCTGCAGAAGAAGACTGA+GGG - chr5_1:46966874-46966893 Msa0733630:intron 40.0%
AGGATCAGTTGAACATCTGT+CGG + chr5_1:46966904-46966923 None:intergenic 40.0%
AGATGTTCAACTGATCCTCT+CGG - chr5_1:46966906-46966925 Msa0733630:intron 40.0%
! TACACAATACGCACCCTTTT+TGG - chr5_1:46966944-46966963 Msa0733630:intron 40.0%
AAGCAAGGAGAATTGTCACA+TGG - chr5_1:46966990-46967009 Msa0733630:intron 40.0%
AGCAAGGAGAATTGTCACAT+GGG - chr5_1:46966991-46967010 Msa0733630:intron 40.0%
AATTGTCACATGGGAGTAGA+TGG - chr5_1:46967000-46967019 Msa0733630:intron 40.0%
TCAATTCCTGCTAATCGTCA+TGG - chr5_1:46965683-46965702 Msa0733630:three_prime_UTR 40.0%
! AAAGTACCTCAAACTCGCAA+AGG - chr5_1:46965707-46965726 Msa0733630:three_prime_UTR 40.0%
GAAGCCAACGAAAGTTACAA+AGG - chr5_1:46965811-46965830 Msa0733630:three_prime_UTR 40.0%
AAACAGATTAAGGCTCCGTT+TGG - chr5_1:46965987-46966006 Msa0733630:three_prime_UTR 40.0%
! GGACTTGATTAGGGTCTTTT+TGG + chr5_1:46966196-46966215 None:intergenic 40.0%
GGAAGCCATTAACACTACAA+CGG - chr5_1:46966690-46966709 Msa0733630:intron 40.0%
GAAGCCATTAACACTACAAC+GGG - chr5_1:46966691-46966710 Msa0733630:intron 40.0%
!! GAAGCTAAGGGTTGATTTTG+AGG - chr5_1:46966797-46966816 Msa0733630:intron 40.0%
TTAAACCAACCCTCTATGCA+TGG + chr5_1:46966826-46966845 None:intergenic 40.0%
!! TGCATAGAGGGTTGGTTTAA+TGG - chr5_1:46966826-46966845 Msa0733630:intron 40.0%
ATATCTGCAGAAGAAGACTG+AGG - chr5_1:46966873-46966892 Msa0733630:intron 40.0%
TATCTGCAGAAGAAGACTGA+GGG - chr5_1:46966874-46966893 Msa0733630:intron 40.0%
AGGATCAGTTGAACATCTGT+CGG + chr5_1:46966904-46966923 None:intergenic 40.0%
AGATGTTCAACTGATCCTCT+CGG - chr5_1:46966906-46966925 Msa0733630:intron 40.0%
! TACACAATACGCACCCTTTT+TGG - chr5_1:46966944-46966963 Msa0733630:intron 40.0%
AAGCAAGGAGAATTGTCACA+TGG - chr5_1:46966990-46967009 Msa0733630:intron 40.0%
AGCAAGGAGAATTGTCACAT+GGG - chr5_1:46966991-46967010 Msa0733630:intron 40.0%
AATTGTCACATGGGAGTAGA+TGG - chr5_1:46967000-46967019 Msa0733630:intron 40.0%
ACTTTGCCATGACGATTAGC+AGG + chr5_1:46965692-46965711 None:intergenic 45.0%
ACTTGTAAGCACTTGTACGG+TGG - chr5_1:46966669-46966688 Msa0733630:intron 45.0%
TCCTCCCGTTGTAGTGTTAA+TGG + chr5_1:46966698-46966717 None:intergenic 45.0%
TTTGAGGACACCATGCATAG+AGG - chr5_1:46966813-46966832 Msa0733630:intron 45.0%
TTGAGGACACCATGCATAGA+GGG - chr5_1:46966814-46966833 Msa0733630:intron 45.0%
!! GAGGGTTGGTTTAATGGCAT+TGG - chr5_1:46966832-46966851 Msa0733630:intron 45.0%
! ATGGCATTGGATCAGTATCG+AGG - chr5_1:46966845-46966864 Msa0733630:intron 45.0%
GGATCAGTTGAACATCTGTC+GGG + chr5_1:46966903-46966922 None:intergenic 45.0%
! AATGATTTTTCACGGCCGAG+AGG + chr5_1:46966924-46966943 None:intergenic 45.0%
ACTTTGCCATGACGATTAGC+AGG + chr5_1:46965692-46965711 None:intergenic 45.0%
ACTTGTAAGCACTTGTACGG+TGG - chr5_1:46966669-46966688 Msa0733630:intron 45.0%
TCCTCCCGTTGTAGTGTTAA+TGG + chr5_1:46966698-46966717 None:intergenic 45.0%
TTTGAGGACACCATGCATAG+AGG - chr5_1:46966813-46966832 Msa0733630:intron 45.0%
TTGAGGACACCATGCATAGA+GGG - chr5_1:46966814-46966833 Msa0733630:intron 45.0%
!! GAGGGTTGGTTTAATGGCAT+TGG - chr5_1:46966832-46966851 Msa0733630:intron 45.0%
! ATGGCATTGGATCAGTATCG+AGG - chr5_1:46966845-46966864 Msa0733630:intron 45.0%
GGATCAGTTGAACATCTGTC+GGG + chr5_1:46966903-46966922 None:intergenic 45.0%
! AATGATTTTTCACGGCCGAG+AGG + chr5_1:46966924-46966943 None:intergenic 45.0%
GCCATTAACACTACAACGGG+AGG - chr5_1:46966694-46966713 Msa0733630:intron 50.0%
GCCATTAACACTACAACGGG+AGG - chr5_1:46966694-46966713 Msa0733630:intron 50.0%
GGACACCATGCATAGAGGGT+TGG - chr5_1:46966818-46966837 Msa0733630:intron 55.0%
GGACACCATGCATAGAGGGT+TGG - chr5_1:46966818-46966837 Msa0733630:intron 55.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 46965534 46967372 46965534 ID=Msa0733630;Name=Msa0733630
chr5_1 mRNA 46965534 46967372 46965534 ID=Msa0733630-mRNA-1;Parent=Msa0733630;Name=Msa0733630-mRNA-1;_AED=0.18;_eAED=0.18;_QI=104|1|1|1|0.5|0.66|3|703|48
chr5_1 exon 46965534 46966239 46965534 ID=Msa0733630-mRNA-1:exon:13441;Parent=Msa0733630-mRNA-1
chr5_1 exon 46967056 46967108 46967056 ID=Msa0733630-mRNA-1:exon:13440;Parent=Msa0733630-mRNA-1
chr5_1 exon 46967178 46967372 46967178 ID=Msa0733630-mRNA-1:exon:13439;Parent=Msa0733630-mRNA-1
chr5_1 five_prime_UTR 46967269 46967372 46967269 ID=Msa0733630-mRNA-1:five_prime_utr;Parent=Msa0733630-mRNA-1
chr5_1 CDS 46967178 46967268 46967178 ID=Msa0733630-mRNA-1:cds;Parent=Msa0733630-mRNA-1
chr5_1 CDS 46967056 46967108 46967056 ID=Msa0733630-mRNA-1:cds;Parent=Msa0733630-mRNA-1
chr5_1 CDS 46966237 46966239 46966237 ID=Msa0733630-mRNA-1:cds;Parent=Msa0733630-mRNA-1
chr5_1 three_prime_UTR 46965534 46966236 46965534 ID=Msa0733630-mRNA-1:three_prime_utr;Parent=Msa0733630-mRNA-1
Gene Sequence

>Msa0733630

ATGACGCAGAAATCAAAACTTTTCAAGGGCCAAACTAAGAAGAAATCAATTCCTGCTAATCGTCATGGCAAAGTACCTCAAACTCGCAAAGGGAAGAGATTTGTGAAGCCAACGAAAGTTACAAAGGATATGGATGCTGATCGTTAA
Protein sequence

>Msa0733630

MTQKSKLFKGQTKKKSIPANRHGKVPQTRKGKRFVKPTKVTKDMDADR*