Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0737470 | XP_003616397.1 | 100.000 | 47 | 0 | 0 | 7 | 53 | 345 | 391 | 1.99e-23 | 100 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0737470 | G7KBQ2 | 100.000 | 47 | 0 | 0 | 7 | 53 | 345 | 391 | 9.53e-24 | 100 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0114060 | Msa0737470 | 0.806650 | 7.419608e-50 | -8.615850e-47 |
| Msa0657970 | Msa0737470 | 0.801621 | 8.257330e-49 | -8.615850e-47 |
| Msa0709400 | Msa0737470 | 0.809514 | 1.822305e-50 | -8.615850e-47 |
| Msa0737470 | Msa0945970 | 0.804432 | 2.166695e-49 | -8.615850e-47 |
| Msa0737470 | Msa1124960 | 0.807406 | 5.134437e-50 | -8.615850e-47 |
| Msa0737470 | Msa1170410 | 0.840503 | 8.363303e-58 | -8.615850e-47 |
| Msa0737470 | Msa1228590 | 0.803558 | 3.291976e-49 | -8.615850e-47 |
| Msa0737470 | Msa1244600 | 0.812998 | 3.196906e-51 | -8.615850e-47 |
| Msa0737470 | Msa1267430 | 0.800025 | 1.748878e-48 | -8.615850e-47 |
| Msa0159930 | Msa0737470 | 0.808004 | 3.832444e-50 | -8.615850e-47 |
| Msa0475550 | Msa0737470 | 0.803748 | 3.006337e-49 | -8.615850e-47 |
| Msa0477080 | Msa0737470 | 0.816100 | 6.580938e-52 | -8.615850e-47 |
| Msa0287410 | Msa0737470 | 0.809171 | 2.159361e-50 | -8.615850e-47 |
| Msa0493630 | Msa0737470 | 0.805184 | 1.508767e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0737470 | MtrunA17_Chr5g0435171 | 100.000 | 47 | 0 | 0 | 7 | 53 | 345 | 391 | 1.83e-27 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0737470 | AT1G66510.3 | 70.833 | 48 | 13 | 1 | 5 | 52 | 302 | 348 | 1.24e-17 | 73.9 |
| Msa0737470 | AT1G66510.4 | 70.833 | 48 | 13 | 1 | 5 | 52 | 346 | 392 | 1.46e-17 | 73.9 |
| Msa0737470 | AT1G66510.1 | 70.833 | 48 | 13 | 1 | 5 | 52 | 346 | 392 | 1.46e-17 | 73.9 |
| Msa0737470 | AT1G66510.2 | 70.833 | 48 | 13 | 1 | 5 | 52 | 346 | 392 | 1.46e-17 | 73.9 |
Find 23 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACAGTAGATTCATCATTCA+TGG | 0.286708 | 5_1:+54717744 | Msa0737450:intron |
| AAGCTATTAGAGAGCAATCT+AGG | 0.318940 | 5_1:-54717333 | Msa0737470:CDS |
| CAATTTCTCAAGCTTCTGAT+AGG | 0.334528 | 5_1:+54716959 | Msa0737450:intron |
| CAGCAGAACAACGCTGTTGA+TGG | 0.346353 | 5_1:-54717300 | Msa0737470:CDS |
| TGTAATATCTTGGCTACAAA+AGG | 0.360930 | 5_1:-54717103 | Msa0737470:three_prime_UTR |
| TGCGCTATCCATTGCTAAGA+TGG | 0.382642 | 5_1:+54716994 | Msa0737450:intron |
| AATTAAATATCTAAACATAT+TGG | 0.383034 | 5_1:+54716768 | Msa0737450:intron |
| TCAGAAGCTTGAGAAATTGT+TGG | 0.403714 | 5_1:-54716955 | Msa0737470:three_prime_UTR |
| ACAGTGATTGCCACTTCATC+AGG | 0.414921 | 5_1:+54716791 | Msa0737450:intron |
| ATGTGGATCATGTAATATCT+TGG | 0.479613 | 5_1:-54717113 | Msa0737470:three_prime_UTR |
| GCTTGAGAAATTGTTGGACT+AGG | 0.485641 | 5_1:-54716949 | Msa0737470:three_prime_UTR |
| TGTAAAATGTCAGTGTCTAA+AGG | 0.513891 | 5_1:+54717034 | Msa0737450:intron |
| AATTTCTCAAGCTTCTGATA+GGG | 0.516408 | 5_1:+54716960 | Msa0737450:intron |
| TCATCTAACATCTCTACTAC+AGG | 0.528298 | 5_1:+54717157 | Msa0737450:intron |
| GTAATATCTTGGCTACAAAA+GGG | 0.548619 | 5_1:-54717102 | Msa0737470:three_prime_UTR |
| ATGTAAATCCATCTTAGCAA+TGG | 0.552526 | 5_1:-54717002 | Msa0737470:three_prime_UTR |
| AGCTATTAGAGAGCAATCTA+GGG | 0.559485 | 5_1:-54717332 | Msa0737470:CDS |
| TATTAGAGAGCAATCTAGGG+TGG | 0.601228 | 5_1:-54717329 | Msa0737470:CDS |
| GCTCACGCAGTTTAGAAATG+TGG | 0.604959 | 5_1:-54717130 | Msa0737470:exon |
| TTACATGTTGCCTGATGAAG+TGG | 0.607987 | 5_1:-54716801 | Msa0737470:three_prime_UTR |
| ATTAGAGAGCAATCTAGGGT+GGG | 0.617313 | 5_1:-54717328 | Msa0737470:CDS |
| TGTCTAAAGGTCATGCAAAG+TGG | 0.650698 | 5_1:+54717047 | Msa0737450:intron |
| GTACTTTGATGAGAACGATG+AGG | 0.679398 | 5_1:-54717274 | Msa0737470:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATAAAAGATAAGATGATT+TGG | + | chr5_1:54716808-54716827 | Msa0737450:intron | 20.0% |
| !! | AGATAAAAGATAAGATGATT+TGG | + | chr5_1:54716808-54716827 | Msa0737450:intron | 20.0% |
| ! | ATCATGAAAGTAAGAGAATA+TGG | + | chr5_1:54716844-54716863 | Msa0737450:intron | 25.0% |
| ! | ACCAATAAGATGATTGATTA+AGG | + | chr5_1:54717322-54717341 | Msa0737450:intron | 25.0% |
| ! | ATGACTGTTAAAAAACATGT+CGG | - | chr5_1:54717609-54717628 | Msa0737470:intron | 25.0% |
| ! | ATCATGAAAGTAAGAGAATA+TGG | + | chr5_1:54716844-54716863 | Msa0737450:intron | 25.0% |
| ! | ACCAATAAGATGATTGATTA+AGG | + | chr5_1:54717322-54717341 | Msa0737450:intron | 25.0% |
| ! | ATGACTGTTAAAAAACATGT+CGG | - | chr5_1:54717609-54717628 | Msa0737470:intron | 25.0% |
| GCCTTAATCAATCATCTTAT+TGG | - | chr5_1:54717318-54717337 | Msa0737470:CDS | 30.0% | |
| ATGTGGATCATGTAATATCT+TGG | - | chr5_1:54717405-54717424 | Msa0737470:intron | 30.0% | |
| TGTAATATCTTGGCTACAAA+AGG | - | chr5_1:54717415-54717434 | Msa0737470:intron | 30.0% | |
| GTAATATCTTGGCTACAAAA+GGG | - | chr5_1:54717416-54717435 | Msa0737470:intron | 30.0% | |
| TGTAAAATGTCAGTGTCTAA+AGG | + | chr5_1:54717487-54717506 | Msa0737450:intron | 30.0% | |
| ATGTAAATCCATCTTAGCAA+TGG | - | chr5_1:54717516-54717535 | Msa0737470:intron | 30.0% | |
| AATTTCTCAAGCTTCTGATA+GGG | + | chr5_1:54717561-54717580 | Msa0737450:intron | 30.0% | |
| GCCTTAATCAATCATCTTAT+TGG | - | chr5_1:54717318-54717337 | Msa0737470:CDS | 30.0% | |
| ATGTGGATCATGTAATATCT+TGG | - | chr5_1:54717405-54717424 | Msa0737470:intron | 30.0% | |
| TGTAATATCTTGGCTACAAA+AGG | - | chr5_1:54717415-54717434 | Msa0737470:intron | 30.0% | |
| GTAATATCTTGGCTACAAAA+GGG | - | chr5_1:54717416-54717435 | Msa0737470:intron | 30.0% | |
| TGTAAAATGTCAGTGTCTAA+AGG | + | chr5_1:54717487-54717506 | Msa0737450:intron | 30.0% | |
| ATGTAAATCCATCTTAGCAA+TGG | - | chr5_1:54717516-54717535 | Msa0737470:intron | 30.0% | |
| AATTTCTCAAGCTTCTGATA+GGG | + | chr5_1:54717561-54717580 | Msa0737450:intron | 30.0% | |
| AAGCTATTAGAGAGCAATCT+AGG | - | chr5_1:54717185-54717204 | Msa0737470:intron | 35.0% | |
| AGCTATTAGAGAGCAATCTA+GGG | - | chr5_1:54717186-54717205 | Msa0737470:intron | 35.0% | |
| TCATCTAACATCTCTACTAC+AGG | + | chr5_1:54717364-54717383 | Msa0737450:intron | 35.0% | |
| CAATTTCTCAAGCTTCTGAT+AGG | + | chr5_1:54717562-54717581 | Msa0737450:intron | 35.0% | |
| TCAGAAGCTTGAGAAATTGT+TGG | - | chr5_1:54717563-54717582 | Msa0737470:intron | 35.0% | |
| ! | TAAAAAACATGTCGGCTAGA+CGG | - | chr5_1:54717617-54717636 | Msa0737470:intron | 35.0% |
| AAGCTATTAGAGAGCAATCT+AGG | - | chr5_1:54717185-54717204 | Msa0737470:intron | 35.0% | |
| AGCTATTAGAGAGCAATCTA+GGG | - | chr5_1:54717186-54717205 | Msa0737470:intron | 35.0% | |
| TCATCTAACATCTCTACTAC+AGG | + | chr5_1:54717364-54717383 | Msa0737450:intron | 35.0% | |
| CAATTTCTCAAGCTTCTGAT+AGG | + | chr5_1:54717562-54717581 | Msa0737450:intron | 35.0% | |
| TCAGAAGCTTGAGAAATTGT+TGG | - | chr5_1:54717563-54717582 | Msa0737470:intron | 35.0% | |
| ! | TAAAAAACATGTCGGCTAGA+CGG | - | chr5_1:54717617-54717636 | Msa0737470:intron | 35.0% |
| ! | CTTTCCTTTTCTTGCAGACA+AGG | - | chr5_1:54717153-54717172 | Msa0737470:CDS | 40.0% |
| ACTTCCTTGTCTGCAAGAAA+AGG | + | chr5_1:54717160-54717179 | Msa0737450:intron | 40.0% | |
| TATTAGAGAGCAATCTAGGG+TGG | - | chr5_1:54717189-54717208 | Msa0737470:intron | 40.0% | |
| ! | ATTAGAGAGCAATCTAGGGT+GGG | - | chr5_1:54717190-54717209 | Msa0737470:intron | 40.0% |
| GTACTTTGATGAGAACGATG+AGG | - | chr5_1:54717244-54717263 | Msa0737470:intron | 40.0% | |
| !!! | GCTACAAAAGGGTGTTTTTG+AGG | - | chr5_1:54717427-54717446 | Msa0737470:intron | 40.0% |
| TGTCTAAAGGTCATGCAAAG+TGG | + | chr5_1:54717474-54717493 | Msa0737450:intron | 40.0% | |
| GCTTGAGAAATTGTTGGACT+AGG | - | chr5_1:54717569-54717588 | Msa0737470:intron | 40.0% | |
| TTACATGTTGCCTGATGAAG+TGG | - | chr5_1:54717717-54717736 | Msa0737470:intron | 40.0% | |
| ! | CTTTCCTTTTCTTGCAGACA+AGG | - | chr5_1:54717153-54717172 | Msa0737470:CDS | 40.0% |
| ACTTCCTTGTCTGCAAGAAA+AGG | + | chr5_1:54717160-54717179 | Msa0737450:intron | 40.0% | |
| TATTAGAGAGCAATCTAGGG+TGG | - | chr5_1:54717189-54717208 | Msa0737470:intron | 40.0% | |
| ! | ATTAGAGAGCAATCTAGGGT+GGG | - | chr5_1:54717190-54717209 | Msa0737470:intron | 40.0% |
| GTACTTTGATGAGAACGATG+AGG | - | chr5_1:54717244-54717263 | Msa0737470:intron | 40.0% | |
| !!! | GCTACAAAAGGGTGTTTTTG+AGG | - | chr5_1:54717427-54717446 | Msa0737470:intron | 40.0% |
| TGTCTAAAGGTCATGCAAAG+TGG | + | chr5_1:54717474-54717493 | Msa0737450:intron | 40.0% | |
| GCTTGAGAAATTGTTGGACT+AGG | - | chr5_1:54717569-54717588 | Msa0737470:intron | 40.0% | |
| TTACATGTTGCCTGATGAAG+TGG | - | chr5_1:54717717-54717736 | Msa0737470:intron | 40.0% | |
| GCTCACGCAGTTTAGAAATG+TGG | - | chr5_1:54717388-54717407 | Msa0737470:intron | 45.0% | |
| TGCGCTATCCATTGCTAAGA+TGG | + | chr5_1:54717527-54717546 | Msa0737450:intron | 45.0% | |
| ! | ACAGTGATTGCCACTTCATC+AGG | + | chr5_1:54717730-54717749 | Msa0737450:intron | 45.0% |
| GCTCACGCAGTTTAGAAATG+TGG | - | chr5_1:54717388-54717407 | Msa0737470:intron | 45.0% | |
| TGCGCTATCCATTGCTAAGA+TGG | + | chr5_1:54717527-54717546 | Msa0737450:intron | 45.0% | |
| ! | ACAGTGATTGCCACTTCATC+AGG | + | chr5_1:54717730-54717749 | Msa0737450:intron | 45.0% |
| CAGCAGAACAACGCTGTTGA+TGG | - | chr5_1:54717218-54717237 | Msa0737470:intron | 50.0% | |
| CAGCAGAACAACGCTGTTGA+TGG | - | chr5_1:54717218-54717237 | Msa0737470:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 54716776 | 54717764 | 54716776 | ID=Msa0737470;Name=Msa0737470 |
| chr5_1 | mRNA | 54716776 | 54717764 | 54716776 | ID=Msa0737470-mRNA-1;Parent=Msa0737470;Name=Msa0737470-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0.5|0.33|0.66|1|1|3|362|53 |
| chr5_1 | exon | 54716776 | 54717185 | 54716776 | ID=Msa0737470-mRNA-1:exon:15406;Parent=Msa0737470-mRNA-1 |
| chr5_1 | exon | 54717275 | 54717370 | 54717275 | ID=Msa0737470-mRNA-1:exon:15405;Parent=Msa0737470-mRNA-1 |
| chr5_1 | exon | 54717747 | 54717764 | 54717747 | ID=Msa0737470-mRNA-1:exon:15404;Parent=Msa0737470-mRNA-1 |
| chr5_1 | CDS | 54717747 | 54717764 | 54717747 | ID=Msa0737470-mRNA-1:cds;Parent=Msa0737470-mRNA-1 |
| chr5_1 | CDS | 54717275 | 54717370 | 54717275 | ID=Msa0737470-mRNA-1:cds;Parent=Msa0737470-mRNA-1 |
| chr5_1 | CDS | 54717138 | 54717185 | 54717138 | ID=Msa0737470-mRNA-1:cds;Parent=Msa0737470-mRNA-1 |
| chr5_1 | three_prime_UTR | 54716776 | 54717137 | 54716776 | ID=Msa0737470-mRNA-1:three_prime_utr;Parent=Msa0737470-mRNA-1 |
| Gene Sequence |
| Protein sequence |
