AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0750990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0750990 MtrunA17_Chr5g0395061 95.131 267 7 1 1 267 1 261 0.0 532
Msa0750990 MtrunA17_Chr6g0468311 75.000 48 6 1 1 48 1 42 5.31e-18 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0750990 AT1G64980.2 78.277 267 55 2 3 267 12 277 3.68e-163 453
Msa0750990 AT1G64980.1 79.401 267 45 3 1 267 1 257 1.65e-162 451
Msa0750990 AT1G64980.3 79.401 267 45 3 1 267 1 257 1.65e-162 451

Find 91 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCCTCCTTTCTTTCTTTC+TGG 0.032183 5_2:+4581596 Msa0750990:five_prime_UTR
CTCAGATCTGATTGCTTAAT+TGG 0.085095 5_2:-4582439 None:intergenic
TCCATCTTTGTAGTCTCTTT+TGG 0.193307 5_2:-4582667 None:intergenic
ATTGAAGACCAATTCTTTCT+AGG 0.195136 5_2:-4582712 None:intergenic
TCTCTATTTAGCTGATATTA+AGG 0.242104 5_2:+4582585 Msa0750990:CDS
ACGTGAAGACATTGCATTTC+AGG 0.259904 5_2:+4582366 Msa0750990:CDS
ATTTCCTTGAAGGACATAAT+AGG 0.275347 5_2:+4582869 Msa0750990:CDS
AAGATTCTTAACACCTAGTT+TGG 0.296665 5_2:+4582522 Msa0750990:CDS
TGTTTAGAGTCAGTCAATTA+TGG 0.301013 5_2:+4583090 Msa0750990:three_prime_UTR
TGGCTTGAGGATGATGAAAT+TGG 0.336305 5_2:+4582826 Msa0750990:CDS
GGAGTAAGAACCTTGTTCTT+AGG 0.346332 5_2:-4582757 None:intergenic
TTTAGCTGATATTAAGGAAT+TGG 0.346940 5_2:+4582591 Msa0750990:CDS
TAGAGATTTGAAGATGCATT+TGG 0.347816 5_2:+4583048 Msa0750990:exon
TTGCGATTATGCATTGTTCT+TGG 0.347846 5_2:+4583335 Msa0750990:three_prime_UTR
CTTTCCTTCATAGGTTTCAA+TGG 0.348106 5_2:+4582806 Msa0750990:CDS
GAGGGCCGTGGTTTGAGGCT+TGG 0.352682 5_2:+4582944 Msa0750990:CDS
AGACTGGTGCTTTCCTTCAT+AGG 0.356440 5_2:+4582797 Msa0750990:CDS
TGTGATTGATTTGAAGCTAT+TGG 0.360642 5_2:-4581419 None:intergenic
TCGGGGAGGGCCGTGGTTTG+AGG 0.366626 5_2:+4582939 Msa0750990:CDS
ATACTGTATCGAGTAAATCT+AGG 0.369673 5_2:-4581445 None:intergenic
ACGGCCCTCCCCGAGTATAA+TGG 0.377630 5_2:-4582930 None:intergenic
TTATTCTTAGACCTCTTGTC+AGG 0.383421 5_2:-4583275 None:intergenic
ATTGCGAATTTGCTGATCTA+TGG 0.387264 5_2:+4582971 Msa0750990:CDS
TTTCCTTGAAGGACATAATA+GGG 0.389168 5_2:+4582870 Msa0750990:CDS
TACTAGTTGGTAATGGTGAT+TGG 0.390769 5_2:-4582270 None:intergenic
TAGAAAGAATTGGTCTTCAA+TGG 0.393256 5_2:+4582714 Msa0750990:CDS
CAGCCCTATTATGTCCTTCA+AGG 0.399988 5_2:-4582873 None:intergenic
TTAATTGGTATGATCTCAAC+AGG 0.402966 5_2:-4582424 None:intergenic
TCAAGGTTGGGCTATGTTTG+TGG 0.418527 5_2:+4582552 Msa0750990:CDS
CAATTGTTGTTGGACTTGTT+TGG 0.421205 5_2:-4581573 None:intergenic
ATTTGTTTAATGACCTATGT+AGG 0.427955 5_2:+4583251 Msa0750990:three_prime_UTR
CCTAGTTTGGCAAATTATCA+AGG 0.430049 5_2:+4582535 Msa0750990:CDS
CCTTGATAATTTGCCAAACT+AGG 0.430154 5_2:-4582535 None:intergenic
ATATTCTATAATCCAGCTAT+TGG 0.437101 5_2:+4581358 Msa0750990:five_prime_UTR
TTTGGCAAATTATCAAGGTT+GGG 0.439482 5_2:+4582540 Msa0750990:CDS
AAACCCTTCAAGATCTTTGT+GGG 0.439682 5_2:+4582334 Msa0750990:CDS
GTCTGTGAATTCACAGCATC+AGG 0.443576 5_2:-4582778 None:intergenic
CTGTGTATCCTAGAAAGAAT+TGG 0.452193 5_2:+4582704 Msa0750990:CDS
TTTGTTTGGAATTTCCTTGA+AGG 0.455406 5_2:+4582859 Msa0750990:CDS
CGAGTATAATGGATAGCCTT+AGG 0.455563 5_2:-4582919 None:intergenic
GAAGAGAAATAGAGAAAGAG+AGG 0.456795 5_2:-4581532 None:intergenic
GTTTGGCAAATTATCAAGGT+TGG 0.457948 5_2:+4582539 Msa0750990:CDS
AGCTTCAATATAGTTTGTGC+AGG 0.461318 5_2:+4583380 Msa0750990:three_prime_UTR
GAAACCCTTCAAGATCTTTG+TGG 0.462717 5_2:+4582333 Msa0750990:CDS
AAGGAGGGGACAATTGTTGT+TGG 0.464646 5_2:-4581583 None:intergenic
ATGATGCAAGTTATGAGAAT+AGG 0.469528 5_2:+4583164 Msa0750990:three_prime_UTR
ATTCCATTACCATTACTAGT+TGG 0.470434 5_2:-4582283 None:intergenic
TCAAGGAAATTCCAAACAAA+AGG 0.473734 5_2:-4582856 None:intergenic
ATTATACTCGGGGAGGGCCG+TGG 0.478358 5_2:+4582932 Msa0750990:CDS
CTAAGGCTATCCATTATACT+CGG 0.478464 5_2:+4582920 Msa0750990:CDS
GTCGTCATTCAATCATGAAA+AGG 0.493012 5_2:+4582392 Msa0750990:CDS
TTACCATTACTAGTTGGTAA+TGG 0.495217 5_2:-4582277 None:intergenic
TATATTGCAATACCAATAGC+TGG 0.497053 5_2:-4581370 None:intergenic
TCCAAAAGAGACTACAAAGA+TGG 0.508455 5_2:+4582666 Msa0750990:CDS
GGAATTGGTAGATTTGATTG+AGG 0.511326 5_2:+4582606 Msa0750990:CDS
TTACCAACTAGTAATGGTAA+TGG 0.521531 5_2:+4582280 Msa0750990:CDS
GGTCTTTATTGGCGTGAAAG+AGG 0.522858 5_2:+4582469 Msa0750990:CDS
TAATTACCAGAAAGAAAGAA+AGG 0.528833 5_2:-4581602 None:intergenic
TACCAGAAAGAAAGAAAGGA+GGG 0.529239 5_2:-4581598 None:intergenic
CAAGCCATTGAAACCTATGA+AGG 0.539155 5_2:-4582810 None:intergenic
ATAACCCACAAAGATCTTGA+AGG 0.541294 5_2:-4582338 None:intergenic
TACTGTATCGAGTAAATCTA+GGG 0.549887 5_2:-4581444 None:intergenic
TCATAGGTTTCAATGGCTTG+AGG 0.551220 5_2:+4582813 Msa0750990:CDS
CCTCTTGTCAGGGCCTACAT+AGG 0.556427 5_2:-4583264 None:intergenic
AAAATCAAATACATCATGAA+AGG 0.558701 5_2:-4583226 None:intergenic
TTCTTCCAAGCCTCAAACCA+CGG 0.559243 5_2:-4582949 None:intergenic
TAGAGAAAGAGAGGATTGAG+CGG 0.561034 5_2:-4581523 None:intergenic
TAAGGCTATCCATTATACTC+GGG 0.565710 5_2:+4582921 Msa0750990:CDS
TGATCTATGGCTGAATGAGA+TGG 0.566426 5_2:+4582984 Msa0750990:CDS
TAACCCACAAAGATCTTGAA+GGG 0.577120 5_2:-4582337 None:intergenic
CTGTGGTCATCCTAAGAACA+AGG 0.579621 5_2:+4582747 Msa0750990:CDS
TATTCTTAGACCTCTTGTCA+GGG 0.580570 5_2:-4583274 None:intergenic
TGATCCAACTACTTCACCTA+AGG 0.585896 5_2:+4582903 Msa0750990:CDS
TCACCATTACCAACTAGTAA+TGG 0.590252 5_2:+4582274 Msa0750990:CDS
ATAGCCTTAGGTGAAGTAGT+TGG 0.594477 5_2:-4582907 None:intergenic
TATAACCCTCCCTCCACTGT+CGG 0.604729 5_2:+4581485 Msa0750990:five_prime_UTR
CCTATGTAGGCCCTGACAAG+AGG 0.608691 5_2:+4583264 Msa0750990:three_prime_UTR
ACATGTTCACCGACAGTGGA+GGG 0.612323 5_2:-4581494 None:intergenic
ACGCACATGTTCACCGACAG+TGG 0.617473 5_2:-4581498 None:intergenic
CACATGTTCACCGACAGTGG+AGG 0.623716 5_2:-4581495 None:intergenic
GATGCTGTGAATTCACAGAC+TGG 0.623826 5_2:+4582781 Msa0750990:CDS
AAAGAGACTACAAAGATGGA+TGG 0.623848 5_2:+4582670 Msa0750990:CDS
GTTCACCGACAGTGGAGGGA+GGG 0.634569 5_2:-4581490 None:intergenic
TTACCAGAAAGAAAGAAAGG+AGG 0.643019 5_2:-4581599 None:intergenic
GCTATCCATTATACTCGGGG+AGG 0.652245 5_2:+4582925 Msa0750990:CDS
TGTTCACCGACAGTGGAGGG+AGG 0.653233 5_2:-4581491 None:intergenic
ACCAGAAAGAAAGAAAGGAG+GGG 0.656464 5_2:-4581597 None:intergenic
ACAGAGATGAGTTTGAGCAA+TGG 0.659058 5_2:+4582241 Msa0750990:intron
CTATCCATTATACTCGGGGA+GGG 0.668674 5_2:+4582926 Msa0750990:CDS
AAGGCTATCCATTATACTCG+GGG 0.680190 5_2:+4582922 Msa0750990:CDS
TGAAATGCAATGTCTTCACG+TGG 0.692922 5_2:-4582364 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTATATGATCAAA+TGG + chr5_2:4581986-4582005 Msa0750990:intron 10.0%
!!! TTTTTTTTATATGATCAAAT+GGG + chr5_2:4581987-4582006 Msa0750990:intron 10.0%
!!! TTTTTTTTTATATGATCAAA+TGG + chr5_2:4581986-4582005 Msa0750990:intron 10.0%
!!! TTTTTTTTATATGATCAAAT+GGG + chr5_2:4581987-4582006 Msa0750990:intron 10.0%
!!! TTTTTTGTATATGATCAAAT+GGG + chr5_2:4581779-4581798 Msa0750990:intron 15.0%
!! TTTGAATTATTTCAACAATT+GGG + chr5_2:4581819-4581838 Msa0750990:intron 15.0%
!!! TTTTTTCTATATGATCAAAT+GGG + chr5_2:4581881-4581900 Msa0750990:intron 15.0%
!! ATTTGAATTATTACAACAAT+TGG + chr5_2:4581921-4581940 Msa0750990:intron 15.0%
!! TTTGAATTATTACAACAATT+GGG + chr5_2:4581922-4581941 Msa0750990:intron 15.0%
!! AGAAAAAAAACAACAAATTA+AGG - chr5_2:4582220-4582239 None:intergenic 15.0%
!!! ATTATCAGATTTTTCTTTTT+TGG - chr5_2:4583028-4583047 None:intergenic 15.0%
!!! TTTTTTGTATATGATCAAAT+GGG + chr5_2:4581779-4581798 Msa0750990:intron 15.0%
!! TTTGAATTATTTCAACAATT+GGG + chr5_2:4581819-4581838 Msa0750990:intron 15.0%
!!! TTTTTTCTATATGATCAAAT+GGG + chr5_2:4581881-4581900 Msa0750990:intron 15.0%
!! ATTTGAATTATTACAACAAT+TGG + chr5_2:4581921-4581940 Msa0750990:intron 15.0%
!! TTTGAATTATTACAACAATT+GGG + chr5_2:4581922-4581941 Msa0750990:intron 15.0%
!! AGAAAAAAAACAACAAATTA+AGG - chr5_2:4582220-4582239 None:intergenic 15.0%
!!! ATTATCAGATTTTTCTTTTT+TGG - chr5_2:4583028-4583047 None:intergenic 15.0%
!!! GTTTTTTGTATATGATCAAA+TGG + chr5_2:4581778-4581797 Msa0750990:intron 20.0%
!! CTTTGAATTATTTCAACAAT+TGG + chr5_2:4581818-4581837 Msa0750990:intron 20.0%
!!! GTTTTTTCTATATGATCAAA+TGG + chr5_2:4581880-4581899 Msa0750990:intron 20.0%
!! TATCACATTATTAAAACAGT+TGG + chr5_2:4582097-4582116 Msa0750990:intron 20.0%
!! AAAATCAAATACATCATGAA+AGG - chr5_2:4583229-4583248 None:intergenic 20.0%
!!! GTTTTTTGTATATGATCAAA+TGG + chr5_2:4581778-4581797 Msa0750990:intron 20.0%
!! CTTTGAATTATTTCAACAAT+TGG + chr5_2:4581818-4581837 Msa0750990:intron 20.0%
!!! GTTTTTTCTATATGATCAAA+TGG + chr5_2:4581880-4581899 Msa0750990:intron 20.0%
!! TATCACATTATTAAAACAGT+TGG + chr5_2:4582097-4582116 Msa0750990:intron 20.0%
!! AAAATCAAATACATCATGAA+AGG - chr5_2:4583229-4583248 None:intergenic 20.0%
! ATATTCTATAATCCAGCTAT+TGG + chr5_2:4581358-4581377 Msa0750990:five_prime_UTR 25.0%
! TAATTACCAGAAAGAAAGAA+AGG - chr5_2:4581605-4581624 None:intergenic 25.0%
! TATCACATTATTACAACAAC+TGG + chr5_2:4581717-4581736 Msa0750990:intron 25.0%
! ATCACATTATTACAACAACT+GGG + chr5_2:4581718-4581737 Msa0750990:intron 25.0%
!! TTTCAACAATTGGGTTATTT+TGG + chr5_2:4581828-4581847 Msa0750990:intron 25.0%
!! TCTCTAAAAGAACAAATCAA+TGG - chr5_2:4582045-4582064 None:intergenic 25.0%
!!! TTTTTTCTGTCATGATCAAA+TGG + chr5_2:4582160-4582179 Msa0750990:intron 25.0%
!! TTTTTCTGTCATGATCAAAT+GGG + chr5_2:4582161-4582180 Msa0750990:intron 25.0%
! TCTCTATTTAGCTGATATTA+AGG + chr5_2:4582585-4582604 Msa0750990:CDS 25.0%
! TTTAGCTGATATTAAGGAAT+TGG + chr5_2:4582591-4582610 Msa0750990:CDS 25.0%
! ATTTGTTTAATGACCTATGT+AGG + chr5_2:4583251-4583270 Msa0750990:three_prime_UTR 25.0%
!!! ATTGAGTGTTTTAATCAACA+AGG + chr5_2:4583304-4583323 Msa0750990:three_prime_UTR 25.0%
! ATATTCTATAATCCAGCTAT+TGG + chr5_2:4581358-4581377 Msa0750990:five_prime_UTR 25.0%
! TAATTACCAGAAAGAAAGAA+AGG - chr5_2:4581605-4581624 None:intergenic 25.0%
! TATCACATTATTACAACAAC+TGG + chr5_2:4581717-4581736 Msa0750990:intron 25.0%
! ATCACATTATTACAACAACT+GGG + chr5_2:4581718-4581737 Msa0750990:intron 25.0%
!! TTTCAACAATTGGGTTATTT+TGG + chr5_2:4581828-4581847 Msa0750990:intron 25.0%
!! TCTCTAAAAGAACAAATCAA+TGG - chr5_2:4582045-4582064 None:intergenic 25.0%
!!! TTTTTTCTGTCATGATCAAA+TGG + chr5_2:4582160-4582179 Msa0750990:intron 25.0%
!! TTTTTCTGTCATGATCAAAT+GGG + chr5_2:4582161-4582180 Msa0750990:intron 25.0%
! TCTCTATTTAGCTGATATTA+AGG + chr5_2:4582585-4582604 Msa0750990:CDS 25.0%
! TTTAGCTGATATTAAGGAAT+TGG + chr5_2:4582591-4582610 Msa0750990:CDS 25.0%
! ATTTGTTTAATGACCTATGT+AGG + chr5_2:4583251-4583270 Msa0750990:three_prime_UTR 25.0%
!!! ATTGAGTGTTTTAATCAACA+AGG + chr5_2:4583304-4583323 Msa0750990:three_prime_UTR 25.0%
TATATTGCAATACCAATAGC+TGG - chr5_2:4581373-4581392 None:intergenic 30.0%
!! TGTGATTGATTTGAAGCTAT+TGG - chr5_2:4581422-4581441 None:intergenic 30.0%
TACTGTATCGAGTAAATCTA+GGG - chr5_2:4581447-4581466 None:intergenic 30.0%
ATACTGTATCGAGTAAATCT+AGG - chr5_2:4581448-4581467 None:intergenic 30.0%
CTTTCACATTTCATTCGATT+GGG + chr5_2:4581634-4581653 Msa0750990:intron 30.0%
!! TTACCATTACTAGTTGGTAA+TGG - chr5_2:4582280-4582299 None:intergenic 30.0%
TTACCAACTAGTAATGGTAA+TGG + chr5_2:4582280-4582299 Msa0750990:CDS 30.0%
ATTCCATTACCATTACTAGT+TGG - chr5_2:4582286-4582305 None:intergenic 30.0%
TTAATTGGTATGATCTCAAC+AGG - chr5_2:4582427-4582446 None:intergenic 30.0%
TGAGAAAAAGTGGTCTTTAT+TGG + chr5_2:4582458-4582477 Msa0750990:CDS 30.0%
AAGATTCTTAACACCTAGTT+TGG + chr5_2:4582522-4582541 Msa0750990:CDS 30.0%
TTTGGCAAATTATCAAGGTT+GGG + chr5_2:4582540-4582559 Msa0750990:CDS 30.0%
ATTGAAGACCAATTCTTTCT+AGG - chr5_2:4582715-4582734 None:intergenic 30.0%
!! TAGAAAGAATTGGTCTTCAA+TGG + chr5_2:4582714-4582733 Msa0750990:CDS 30.0%
!!! TCAATGGTTTTGTATAACTG+TGG + chr5_2:4582730-4582749 Msa0750990:CDS 30.0%
TCAAGGAAATTCCAAACAAA+AGG - chr5_2:4582859-4582878 None:intergenic 30.0%
! TTTGTTTGGAATTTCCTTGA+AGG + chr5_2:4582859-4582878 Msa0750990:CDS 30.0%
ATTTCCTTGAAGGACATAAT+AGG + chr5_2:4582869-4582888 Msa0750990:CDS 30.0%
TTTCCTTGAAGGACATAATA+GGG + chr5_2:4582870-4582889 Msa0750990:CDS 30.0%
TAGAGATTTGAAGATGCATT+TGG + chr5_2:4583048-4583067 Msa0750990:exon 30.0%
! TGTTTAGAGTCAGTCAATTA+TGG + chr5_2:4583090-4583109 Msa0750990:three_prime_UTR 30.0%
ATGATGCAAGTTATGAGAAT+AGG + chr5_2:4583164-4583183 Msa0750990:three_prime_UTR 30.0%
TATATTGCAATACCAATAGC+TGG - chr5_2:4581373-4581392 None:intergenic 30.0%
!! TGTGATTGATTTGAAGCTAT+TGG - chr5_2:4581422-4581441 None:intergenic 30.0%
TACTGTATCGAGTAAATCTA+GGG - chr5_2:4581447-4581466 None:intergenic 30.0%
ATACTGTATCGAGTAAATCT+AGG - chr5_2:4581448-4581467 None:intergenic 30.0%
CTTTCACATTTCATTCGATT+GGG + chr5_2:4581634-4581653 Msa0750990:intron 30.0%
!! TTACCATTACTAGTTGGTAA+TGG - chr5_2:4582280-4582299 None:intergenic 30.0%
TTACCAACTAGTAATGGTAA+TGG + chr5_2:4582280-4582299 Msa0750990:CDS 30.0%
ATTCCATTACCATTACTAGT+TGG - chr5_2:4582286-4582305 None:intergenic 30.0%
TTAATTGGTATGATCTCAAC+AGG - chr5_2:4582427-4582446 None:intergenic 30.0%
TGAGAAAAAGTGGTCTTTAT+TGG + chr5_2:4582458-4582477 Msa0750990:CDS 30.0%
AAGATTCTTAACACCTAGTT+TGG + chr5_2:4582522-4582541 Msa0750990:CDS 30.0%
TTTGGCAAATTATCAAGGTT+GGG + chr5_2:4582540-4582559 Msa0750990:CDS 30.0%
ATTGAAGACCAATTCTTTCT+AGG - chr5_2:4582715-4582734 None:intergenic 30.0%
!! TAGAAAGAATTGGTCTTCAA+TGG + chr5_2:4582714-4582733 Msa0750990:CDS 30.0%
!!! TCAATGGTTTTGTATAACTG+TGG + chr5_2:4582730-4582749 Msa0750990:CDS 30.0%
TCAAGGAAATTCCAAACAAA+AGG - chr5_2:4582859-4582878 None:intergenic 30.0%
! TTTGTTTGGAATTTCCTTGA+AGG + chr5_2:4582859-4582878 Msa0750990:CDS 30.0%
ATTTCCTTGAAGGACATAAT+AGG + chr5_2:4582869-4582888 Msa0750990:CDS 30.0%
TTTCCTTGAAGGACATAATA+GGG + chr5_2:4582870-4582889 Msa0750990:CDS 30.0%
TAGAGATTTGAAGATGCATT+TGG + chr5_2:4583048-4583067 Msa0750990:exon 30.0%
! TGTTTAGAGTCAGTCAATTA+TGG + chr5_2:4583090-4583109 Msa0750990:three_prime_UTR 30.0%
ATGATGCAAGTTATGAGAAT+AGG + chr5_2:4583164-4583183 Msa0750990:three_prime_UTR 30.0%
GAAGAGAAATAGAGAAAGAG+AGG - chr5_2:4581535-4581554 None:intergenic 35.0%
! CAATTGTTGTTGGACTTGTT+TGG - chr5_2:4581576-4581595 None:intergenic 35.0%
TACCAGAAAGAAAGAAAGGA+GGG - chr5_2:4581601-4581620 None:intergenic 35.0%
TTACCAGAAAGAAAGAAAGG+AGG - chr5_2:4581602-4581621 None:intergenic 35.0%
CCAATCGAATGAAATGTGAA+AGG - chr5_2:4581636-4581655 None:intergenic 35.0%
CCTTTCACATTTCATTCGAT+TGG + chr5_2:4581633-4581652 Msa0750990:intron 35.0%
!! TACTAGTTGGTAATGGTGAT+TGG - chr5_2:4582273-4582292 None:intergenic 35.0%
TCACCATTACCAACTAGTAA+TGG + chr5_2:4582274-4582293 Msa0750990:CDS 35.0%
AAACCCTTCAAGATCTTTGT+GGG + chr5_2:4582334-4582353 Msa0750990:CDS 35.0%
TAACCCACAAAGATCTTGAA+GGG - chr5_2:4582340-4582359 None:intergenic 35.0%
ATAACCCACAAAGATCTTGA+AGG - chr5_2:4582341-4582360 None:intergenic 35.0%
GTCGTCATTCAATCATGAAA+AGG + chr5_2:4582392-4582411 Msa0750990:CDS 35.0%
CTCAGATCTGATTGCTTAAT+TGG - chr5_2:4582442-4582461 None:intergenic 35.0%
CAATCAGATCTGAGAAAAAG+TGG + chr5_2:4582448-4582467 Msa0750990:CDS 35.0%
! CCTTGATAATTTGCCAAACT+AGG - chr5_2:4582538-4582557 None:intergenic 35.0%
CCTAGTTTGGCAAATTATCA+AGG + chr5_2:4582535-4582554 Msa0750990:CDS 35.0%
GTTTGGCAAATTATCAAGGT+TGG + chr5_2:4582539-4582558 Msa0750990:CDS 35.0%
GGAATTGGTAGATTTGATTG+AGG + chr5_2:4582606-4582625 Msa0750990:CDS 35.0%
TCCAAAAGAGACTACAAAGA+TGG + chr5_2:4582666-4582685 Msa0750990:CDS 35.0%
! TCCATCTTTGTAGTCTCTTT+TGG - chr5_2:4582670-4582689 None:intergenic 35.0%
AAAGAGACTACAAAGATGGA+TGG + chr5_2:4582670-4582689 Msa0750990:CDS 35.0%
CTGTGTATCCTAGAAAGAAT+TGG + chr5_2:4582704-4582723 Msa0750990:CDS 35.0%
CTTTCCTTCATAGGTTTCAA+TGG + chr5_2:4582806-4582825 Msa0750990:CDS 35.0%
!!! TTGGTTCTGTACCTTTTGTT+TGG + chr5_2:4582845-4582864 Msa0750990:CDS 35.0%
CTAAGGCTATCCATTATACT+CGG + chr5_2:4582920-4582939 Msa0750990:CDS 35.0%
TAAGGCTATCCATTATACTC+GGG + chr5_2:4582921-4582940 Msa0750990:CDS 35.0%
!! ATTGCGAATTTGCTGATCTA+TGG + chr5_2:4582971-4582990 Msa0750990:CDS 35.0%
!!! TTTCTTTTTTGGCTTGAGCA+AGG - chr5_2:4583017-4583036 None:intergenic 35.0%
TATTCTTAGACCTCTTGTCA+GGG - chr5_2:4583277-4583296 None:intergenic 35.0%
TTATTCTTAGACCTCTTGTC+AGG - chr5_2:4583278-4583297 None:intergenic 35.0%
TTGCGATTATGCATTGTTCT+TGG + chr5_2:4583335-4583354 Msa0750990:three_prime_UTR 35.0%
! AGCTTCAATATAGTTTGTGC+AGG + chr5_2:4583380-4583399 Msa0750990:three_prime_UTR 35.0%
! GCTTCAATATAGTTTGTGCA+GGG + chr5_2:4583381-4583400 Msa0750990:three_prime_UTR 35.0%
! CTTCAATATAGTTTGTGCAG+GGG + chr5_2:4583382-4583401 Msa0750990:three_prime_UTR 35.0%
GAAGAGAAATAGAGAAAGAG+AGG - chr5_2:4581535-4581554 None:intergenic 35.0%
! CAATTGTTGTTGGACTTGTT+TGG - chr5_2:4581576-4581595 None:intergenic 35.0%
TACCAGAAAGAAAGAAAGGA+GGG - chr5_2:4581601-4581620 None:intergenic 35.0%
TTACCAGAAAGAAAGAAAGG+AGG - chr5_2:4581602-4581621 None:intergenic 35.0%
CCAATCGAATGAAATGTGAA+AGG - chr5_2:4581636-4581655 None:intergenic 35.0%
CCTTTCACATTTCATTCGAT+TGG + chr5_2:4581633-4581652 Msa0750990:intron 35.0%
!! TACTAGTTGGTAATGGTGAT+TGG - chr5_2:4582273-4582292 None:intergenic 35.0%
TCACCATTACCAACTAGTAA+TGG + chr5_2:4582274-4582293 Msa0750990:CDS 35.0%
AAACCCTTCAAGATCTTTGT+GGG + chr5_2:4582334-4582353 Msa0750990:CDS 35.0%
TAACCCACAAAGATCTTGAA+GGG - chr5_2:4582340-4582359 None:intergenic 35.0%
ATAACCCACAAAGATCTTGA+AGG - chr5_2:4582341-4582360 None:intergenic 35.0%
GTCGTCATTCAATCATGAAA+AGG + chr5_2:4582392-4582411 Msa0750990:CDS 35.0%
CTCAGATCTGATTGCTTAAT+TGG - chr5_2:4582442-4582461 None:intergenic 35.0%
CAATCAGATCTGAGAAAAAG+TGG + chr5_2:4582448-4582467 Msa0750990:CDS 35.0%
! CCTTGATAATTTGCCAAACT+AGG - chr5_2:4582538-4582557 None:intergenic 35.0%
CCTAGTTTGGCAAATTATCA+AGG + chr5_2:4582535-4582554 Msa0750990:CDS 35.0%
GTTTGGCAAATTATCAAGGT+TGG + chr5_2:4582539-4582558 Msa0750990:CDS 35.0%
GGAATTGGTAGATTTGATTG+AGG + chr5_2:4582606-4582625 Msa0750990:CDS 35.0%
TCCAAAAGAGACTACAAAGA+TGG + chr5_2:4582666-4582685 Msa0750990:CDS 35.0%
! TCCATCTTTGTAGTCTCTTT+TGG - chr5_2:4582670-4582689 None:intergenic 35.0%
AAAGAGACTACAAAGATGGA+TGG + chr5_2:4582670-4582689 Msa0750990:CDS 35.0%
CTGTGTATCCTAGAAAGAAT+TGG + chr5_2:4582704-4582723 Msa0750990:CDS 35.0%
CTTTCCTTCATAGGTTTCAA+TGG + chr5_2:4582806-4582825 Msa0750990:CDS 35.0%
!!! TTGGTTCTGTACCTTTTGTT+TGG + chr5_2:4582845-4582864 Msa0750990:CDS 35.0%
CTAAGGCTATCCATTATACT+CGG + chr5_2:4582920-4582939 Msa0750990:CDS 35.0%
TAAGGCTATCCATTATACTC+GGG + chr5_2:4582921-4582940 Msa0750990:CDS 35.0%
!! ATTGCGAATTTGCTGATCTA+TGG + chr5_2:4582971-4582990 Msa0750990:CDS 35.0%
!!! TTTCTTTTTTGGCTTGAGCA+AGG - chr5_2:4583017-4583036 None:intergenic 35.0%
TATTCTTAGACCTCTTGTCA+GGG - chr5_2:4583277-4583296 None:intergenic 35.0%
TTATTCTTAGACCTCTTGTC+AGG - chr5_2:4583278-4583297 None:intergenic 35.0%
TTGCGATTATGCATTGTTCT+TGG + chr5_2:4583335-4583354 Msa0750990:three_prime_UTR 35.0%
TAGAGAAAGAGAGGATTGAG+CGG - chr5_2:4581526-4581545 None:intergenic 40.0%
! GGACTTGTTTGGAAAAAGAG+AGG - chr5_2:4581565-4581584 None:intergenic 40.0%
ACCAGAAAGAAAGAAAGGAG+GGG - chr5_2:4581600-4581619 None:intergenic 40.0%
ACAGAGATGAGTTTGAGCAA+TGG + chr5_2:4582241-4582260 Msa0750990:intron 40.0%
GAAACCCTTCAAGATCTTTG+TGG + chr5_2:4582333-4582352 Msa0750990:CDS 40.0%
TGAAATGCAATGTCTTCACG+TGG - chr5_2:4582367-4582386 None:intergenic 40.0%
ACGTGAAGACATTGCATTTC+AGG + chr5_2:4582366-4582385 Msa0750990:CDS 40.0%
GGAGTAAGAACCTTGTTCTT+AGG - chr5_2:4582760-4582779 None:intergenic 40.0%
CAAGCCATTGAAACCTATGA+AGG - chr5_2:4582813-4582832 None:intergenic 40.0%
TCATAGGTTTCAATGGCTTG+AGG + chr5_2:4582813-4582832 Msa0750990:CDS 40.0%
TGGCTTGAGGATGATGAAAT+TGG + chr5_2:4582826-4582845 Msa0750990:CDS 40.0%
TGATCCAACTACTTCACCTA+AGG + chr5_2:4582903-4582922 Msa0750990:CDS 40.0%
! ATAGCCTTAGGTGAAGTAGT+TGG - chr5_2:4582910-4582929 None:intergenic 40.0%
!! CGAGTATAATGGATAGCCTT+AGG - chr5_2:4582922-4582941 None:intergenic 40.0%
AAGGCTATCCATTATACTCG+GGG + chr5_2:4582922-4582941 Msa0750990:CDS 40.0%
TGATCTATGGCTGAATGAGA+TGG + chr5_2:4582984-4583003 Msa0750990:CDS 40.0%
TAGAGAAAGAGAGGATTGAG+CGG - chr5_2:4581526-4581545 None:intergenic 40.0%
! GGACTTGTTTGGAAAAAGAG+AGG - chr5_2:4581565-4581584 None:intergenic 40.0%
ACCAGAAAGAAAGAAAGGAG+GGG - chr5_2:4581600-4581619 None:intergenic 40.0%
ACAGAGATGAGTTTGAGCAA+TGG + chr5_2:4582241-4582260 Msa0750990:intron 40.0%
GAAACCCTTCAAGATCTTTG+TGG + chr5_2:4582333-4582352 Msa0750990:CDS 40.0%
TGAAATGCAATGTCTTCACG+TGG - chr5_2:4582367-4582386 None:intergenic 40.0%
ACGTGAAGACATTGCATTTC+AGG + chr5_2:4582366-4582385 Msa0750990:CDS 40.0%
GGAGTAAGAACCTTGTTCTT+AGG - chr5_2:4582760-4582779 None:intergenic 40.0%
CAAGCCATTGAAACCTATGA+AGG - chr5_2:4582813-4582832 None:intergenic 40.0%
TCATAGGTTTCAATGGCTTG+AGG + chr5_2:4582813-4582832 Msa0750990:CDS 40.0%
TGGCTTGAGGATGATGAAAT+TGG + chr5_2:4582826-4582845 Msa0750990:CDS 40.0%
TGATCCAACTACTTCACCTA+AGG + chr5_2:4582903-4582922 Msa0750990:CDS 40.0%
! ATAGCCTTAGGTGAAGTAGT+TGG - chr5_2:4582910-4582929 None:intergenic 40.0%
!! CGAGTATAATGGATAGCCTT+AGG - chr5_2:4582922-4582941 None:intergenic 40.0%
AAGGCTATCCATTATACTCG+GGG + chr5_2:4582922-4582941 Msa0750990:CDS 40.0%
TGATCTATGGCTGAATGAGA+TGG + chr5_2:4582984-4583003 Msa0750990:CDS 40.0%
AAGGAGGGGACAATTGTTGT+TGG - chr5_2:4581586-4581605 None:intergenic 45.0%
TCCCCTCCTTTCTTTCTTTC+TGG + chr5_2:4581596-4581615 Msa0750990:five_prime_UTR 45.0%
GGTCTTTATTGGCGTGAAAG+AGG + chr5_2:4582469-4582488 Msa0750990:CDS 45.0%
TCAAGGTTGGGCTATGTTTG+TGG + chr5_2:4582552-4582571 Msa0750990:CDS 45.0%
CTGTGGTCATCCTAAGAACA+AGG + chr5_2:4582747-4582766 Msa0750990:CDS 45.0%
GTCTGTGAATTCACAGCATC+AGG - chr5_2:4582781-4582800 None:intergenic 45.0%
GATGCTGTGAATTCACAGAC+TGG + chr5_2:4582781-4582800 Msa0750990:CDS 45.0%
AGACTGGTGCTTTCCTTCAT+AGG + chr5_2:4582797-4582816 Msa0750990:CDS 45.0%
CAGCCCTATTATGTCCTTCA+AGG - chr5_2:4582876-4582895 None:intergenic 45.0%
CTATCCATTATACTCGGGGA+GGG + chr5_2:4582926-4582945 Msa0750990:CDS 45.0%
TTCTTCCAAGCCTCAAACCA+CGG - chr5_2:4582952-4582971 None:intergenic 45.0%
AAGGAGGGGACAATTGTTGT+TGG - chr5_2:4581586-4581605 None:intergenic 45.0%
TCCCCTCCTTTCTTTCTTTC+TGG + chr5_2:4581596-4581615 Msa0750990:five_prime_UTR 45.0%
GGTCTTTATTGGCGTGAAAG+AGG + chr5_2:4582469-4582488 Msa0750990:CDS 45.0%
TCAAGGTTGGGCTATGTTTG+TGG + chr5_2:4582552-4582571 Msa0750990:CDS 45.0%
CTGTGGTCATCCTAAGAACA+AGG + chr5_2:4582747-4582766 Msa0750990:CDS 45.0%
GTCTGTGAATTCACAGCATC+AGG - chr5_2:4582781-4582800 None:intergenic 45.0%
GATGCTGTGAATTCACAGAC+TGG + chr5_2:4582781-4582800 Msa0750990:CDS 45.0%
AGACTGGTGCTTTCCTTCAT+AGG + chr5_2:4582797-4582816 Msa0750990:CDS 45.0%
CAGCCCTATTATGTCCTTCA+AGG - chr5_2:4582876-4582895 None:intergenic 45.0%
CTATCCATTATACTCGGGGA+GGG + chr5_2:4582926-4582945 Msa0750990:CDS 45.0%
TTCTTCCAAGCCTCAAACCA+CGG - chr5_2:4582952-4582971 None:intergenic 45.0%
TATAACCCTCCCTCCACTGT+CGG + chr5_2:4581485-4581504 Msa0750990:five_prime_UTR 50.0%
ACATGTTCACCGACAGTGGA+GGG - chr5_2:4581497-4581516 None:intergenic 50.0%
GCTATCCATTATACTCGGGG+AGG + chr5_2:4582925-4582944 Msa0750990:CDS 50.0%
TATAACCCTCCCTCCACTGT+CGG + chr5_2:4581485-4581504 Msa0750990:five_prime_UTR 50.0%
ACATGTTCACCGACAGTGGA+GGG - chr5_2:4581497-4581516 None:intergenic 50.0%
GCTATCCATTATACTCGGGG+AGG + chr5_2:4582925-4582944 Msa0750990:CDS 50.0%
CACATGTTCACCGACAGTGG+AGG - chr5_2:4581498-4581517 None:intergenic 55.0%
ACGCACATGTTCACCGACAG+TGG - chr5_2:4581501-4581520 None:intergenic 55.0%
CCTCTTGTCAGGGCCTACAT+AGG - chr5_2:4583267-4583286 None:intergenic 55.0%
CCTATGTAGGCCCTGACAAG+AGG + chr5_2:4583264-4583283 Msa0750990:three_prime_UTR 55.0%
CACATGTTCACCGACAGTGG+AGG - chr5_2:4581498-4581517 None:intergenic 55.0%
ACGCACATGTTCACCGACAG+TGG - chr5_2:4581501-4581520 None:intergenic 55.0%
CCTCTTGTCAGGGCCTACAT+AGG - chr5_2:4583267-4583286 None:intergenic 55.0%
CCTATGTAGGCCCTGACAAG+AGG + chr5_2:4583264-4583283 Msa0750990:three_prime_UTR 55.0%
GTTCACCGACAGTGGAGGGA+GGG - chr5_2:4581493-4581512 None:intergenic 60.0%
TGTTCACCGACAGTGGAGGG+AGG - chr5_2:4581494-4581513 None:intergenic 60.0%
ACGGCCCTCCCCGAGTATAA+TGG - chr5_2:4582933-4582952 None:intergenic 60.0%
ATTATACTCGGGGAGGGCCG+TGG + chr5_2:4582932-4582951 Msa0750990:CDS 60.0%
GTTCACCGACAGTGGAGGGA+GGG - chr5_2:4581493-4581512 None:intergenic 60.0%
TGTTCACCGACAGTGGAGGG+AGG - chr5_2:4581494-4581513 None:intergenic 60.0%
ACGGCCCTCCCCGAGTATAA+TGG - chr5_2:4582933-4582952 None:intergenic 60.0%
ATTATACTCGGGGAGGGCCG+TGG + chr5_2:4582932-4582951 Msa0750990:CDS 60.0%
! GAGGGCCGTGGTTTGAGGCT+TGG + chr5_2:4582944-4582963 Msa0750990:CDS 65.0%
! GAGGGCCGTGGTTTGAGGCT+TGG + chr5_2:4582944-4582963 Msa0750990:CDS 65.0%
TCGGGGAGGGCCGTGGTTTG+AGG + chr5_2:4582939-4582958 Msa0750990:CDS 70.0%
TCGGGGAGGGCCGTGGTTTG+AGG + chr5_2:4582939-4582958 Msa0750990:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 4581346 4583402 4581346 ID=Msa0750990;Name=Msa0750990
chr5_2 mRNA 4581346 4583402 4581346 ID=Msa0750990-mRNA-1;Parent=Msa0750990;Name=Msa0750990-mRNA-1;_AED=0.00;_eAED=0.00;_QI=272|0|0.5|1|0|0.5|2|352|267
chr5_2 exon 4581346 4581617 4581346 ID=Msa0750990-mRNA-1:exon:1713;Parent=Msa0750990-mRNA-1
chr5_2 exon 4582247 4583402 4582247 ID=Msa0750990-mRNA-1:exon:1714;Parent=Msa0750990-mRNA-1
chr5_2 five_prime_UTR 4581346 4581617 4581346 ID=Msa0750990-mRNA-1:five_prime_utr;Parent=Msa0750990-mRNA-1
chr5_2 CDS 4582247 4583050 4582247 ID=Msa0750990-mRNA-1:cds;Parent=Msa0750990-mRNA-1
chr5_2 three_prime_UTR 4583051 4583402 4583051 ID=Msa0750990-mRNA-1:three_prime_utr;Parent=Msa0750990-mRNA-1
Gene Sequence

>Msa0750990

ATGAGTTTGAGCAATGGTAAAAACCAATCACCATTACCAACTAGTAATGGTAATGGAATAGATTCTGCTGAGAATGAGAATGCAAAGAAACCCTTCAAGATCTTTGTGGGTTATGATCCACGTGAAGACATTGCATTTCAGGTATGTCGTCATTCAATCATGAAAAGGTCTTCAATTCCTGTTGAGATCATACCAATTAAGCAATCAGATCTGAGAAAAAGTGGTCTTTATTGGCGTGAAAGAGGTCAATTTGAGAGCACTGAGTTTTCTTTTACAAGATTCTTAACACCTAGTTTGGCAAATTATCAAGGTTGGGCTATGTTTGTGGATTGTGATTTTCTCTATTTAGCTGATATTAAGGAATTGGTAGATTTGATTGAGGATAAGTATGCTATTATGTGTGTTCAACATGATTATACTCCAAAAGAGACTACAAAGATGGATGGTGCTGTTCAAACTGTGTATCCTAGAAAGAATTGGTCTTCAATGGTTTTGTATAACTGTGGTCATCCTAAGAACAAGGTTCTTACTCCTGATGCTGTGAATTCACAGACTGGTGCTTTCCTTCATAGGTTTCAATGGCTTGAGGATGATGAAATTGGTTCTGTACCTTTTGTTTGGAATTTCCTTGAAGGACATAATAGGGCTGTTGAGAATGATCCAACTACTTCACCTAAGGCTATCCATTATACTCGGGGAGGGCCGTGGTTTGAGGCTTGGAAGAATTGCGAATTTGCTGATCTATGGCTGAATGAGATGGAAGAGTACCTTGCTCAAGCCAAAAAAGAAAAATCTGATAATTAG
Protein sequence

>Msa0750990

MSLSNGKNQSPLPTSNGNGIDSAENENAKKPFKIFVGYDPREDIAFQVCRHSIMKRSSIPVEIIPIKQSDLRKSGLYWRERGQFESTEFSFTRFLTPSLANYQGWAMFVDCDFLYLADIKELVDLIEDKYAIMCVQHDYTPKETTKMDGAVQTVYPRKNWSSMVLYNCGHPKNKVLTPDAVNSQTGAFLHRFQWLEDDEIGSVPFVWNFLEGHNRAVENDPTTSPKAIHYTRGGPWFEAWKNCEFADLWLNEMEEYLAQAKKEKSDN*