AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0794030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0794030 MtrunA17_Chr5g0395061 95.131 267 7 1 1 267 1 261 0.0 532
Msa0794030 MtrunA17_Chr6g0468311 75.000 48 6 1 1 48 1 42 5.31e-18 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0794030 AT1G64980.2 78.277 267 55 2 3 267 12 277 3.68e-163 453
Msa0794030 AT1G64980.1 79.401 267 45 3 1 267 1 257 1.65e-162 451
Msa0794030 AT1G64980.3 79.401 267 45 3 1 267 1 257 1.65e-162 451

Find 92 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCCTCCTTTCTTTCTTTC+TGG 0.032183 5_3:+5842586 Msa0794030:five_prime_UTR
CTCAGATCTGATTGCTTAAT+TGG 0.085095 5_3:-5843429 None:intergenic
TCCATCTTTGTAGTCTCTTT+TGG 0.193307 5_3:-5843657 None:intergenic
ATTGAAGACCAATTCTTTCT+AGG 0.195136 5_3:-5843702 None:intergenic
TCTCTATTTAGCTGATATTA+AGG 0.242104 5_3:+5843575 Msa0794030:CDS
ACGTGAAGACATTGCATTTC+AGG 0.259904 5_3:+5843356 Msa0794030:CDS
ATTTCCTTGAAGGACATAAT+AGG 0.275347 5_3:+5843859 Msa0794030:CDS
AAGATTCTTAACACCTAGTT+TGG 0.296665 5_3:+5843512 Msa0794030:CDS
TGTTTAGAGTCAGTCAATTA+TGG 0.301013 5_3:+5844080 Msa0794030:three_prime_UTR
TGGCTTGAGGATGATGAAAT+TGG 0.336305 5_3:+5843816 Msa0794030:CDS
GGAGTAAGAACCTTGTTCTT+AGG 0.346332 5_3:-5843747 None:intergenic
TTTAGCTGATATTAAGGAAT+TGG 0.346940 5_3:+5843581 Msa0794030:CDS
TAGAGATTTGAAGATGCATT+TGG 0.347816 5_3:+5844038 Msa0794030:exon
TTGCGATTATGCATTGTTCT+TGG 0.347846 5_3:+5844318 Msa0794030:three_prime_UTR
CTTTCCTTCATAGGTTTCAA+TGG 0.348106 5_3:+5843796 Msa0794030:CDS
GAGGGCCGTGGTTTGAGGCT+TGG 0.352682 5_3:+5843934 Msa0794030:CDS
AGACTGGTGCTTTCCTTCAT+AGG 0.356440 5_3:+5843787 Msa0794030:CDS
TCGGGGAGGGCCGTGGTTTG+AGG 0.366626 5_3:+5843929 Msa0794030:CDS
ATACTGTATCGAGTAAATCT+AGG 0.369673 5_3:-5842435 None:intergenic
ACGGCCCTCCCCGAGTATAA+TGG 0.377630 5_3:-5843920 None:intergenic
TTATTCTTAGACCTCTTGTC+AGG 0.383421 5_3:-5844258 None:intergenic
ATTGCGAATTTGCTGATCTA+TGG 0.387264 5_3:+5843961 Msa0794030:CDS
TTTCCTTGAAGGACATAATA+GGG 0.389168 5_3:+5843860 Msa0794030:CDS
TACTAGTTGGTAATGGTGAT+TGG 0.390769 5_3:-5843260 None:intergenic
TAGAAAGAATTGGTCTTCAA+TGG 0.393256 5_3:+5843704 Msa0794030:CDS
CAGCCCTATTATGTCCTTCA+AGG 0.399988 5_3:-5843863 None:intergenic
TTAATTGGTATGATCTCAAC+AGG 0.402966 5_3:-5843414 None:intergenic
TCAAGGTTGGGCTATGTTTG+TGG 0.418527 5_3:+5843542 Msa0794030:CDS
ATTTGTTTAATGACCTATGT+AGG 0.427955 5_3:+5844234 Msa0794030:three_prime_UTR
CCTAGTTTGGCAAATTATCA+AGG 0.430049 5_3:+5843525 Msa0794030:CDS
CCTTGATAATTTGCCAAACT+AGG 0.430154 5_3:-5843525 None:intergenic
ATATTCTATAATCCAGCTAT+TGG 0.437101 5_3:+5842356 Msa0794030:five_prime_UTR
TTTGGCAAATTATCAAGGTT+GGG 0.439482 5_3:+5843530 Msa0794030:CDS
AAACCCTTCAAGATCTTTGT+GGG 0.439682 5_3:+5843324 Msa0794030:CDS
CAAGTGTTGTTGGACTTGTT+TGG 0.442730 5_3:-5842563 None:intergenic
GTCTGTGAATTCACAGCATC+AGG 0.443576 5_3:-5843768 None:intergenic
CTGTGTATCCTAGAAAGAAT+TGG 0.452193 5_3:+5843694 Msa0794030:CDS
TTTGTTTGGAATTTCCTTGA+AGG 0.455406 5_3:+5843849 Msa0794030:CDS
CGAGTATAATGGATAGCCTT+AGG 0.455563 5_3:-5843909 None:intergenic
GTTTGGCAAATTATCAAGGT+TGG 0.457948 5_3:+5843529 Msa0794030:CDS
AAGGAGGGGACAAGTGTTGT+TGG 0.460012 5_3:-5842573 None:intergenic
AGCTTCAATATAGTTTGTGC+AGG 0.461318 5_3:+5844363 Msa0794030:three_prime_UTR
GAAACCCTTCAAGATCTTTG+TGG 0.462717 5_3:+5843323 Msa0794030:CDS
ATGATGCAAGTTATGAGAAT+AGG 0.469528 5_3:+5844147 Msa0794030:three_prime_UTR
ATTCCATTACCATTACTAGT+TGG 0.470434 5_3:-5843273 None:intergenic
TCAAGGAAATTCCAAACAAA+AGG 0.473734 5_3:-5843846 None:intergenic
GAAGAGAAATAGAGAAAGAG+AGG 0.476636 5_3:-5842522 None:intergenic
ATTATACTCGGGGAGGGCCG+TGG 0.478358 5_3:+5843922 Msa0794030:CDS
CTAAGGCTATCCATTATACT+CGG 0.478464 5_3:+5843910 Msa0794030:CDS
GTCGTCATTCAATCATGAAA+AGG 0.493012 5_3:+5843382 Msa0794030:CDS
TTACCATTACTAGTTGGTAA+TGG 0.495217 5_3:-5843267 None:intergenic
TATATTGCAATACCAATAGC+TGG 0.497053 5_3:-5842368 None:intergenic
TTGTTGGACTTGTTTGGAAA+AGG 0.507198 5_3:-5842557 None:intergenic
TCCAAAAGAGACTACAAAGA+TGG 0.508455 5_3:+5843656 Msa0794030:CDS
GGAATTGGTAGATTTGATTG+AGG 0.511326 5_3:+5843596 Msa0794030:CDS
TTACCAACTAGTAATGGTAA+TGG 0.521531 5_3:+5843270 Msa0794030:CDS
GGTCTTTATTGGCGTGAAAG+AGG 0.522858 5_3:+5843459 Msa0794030:CDS
TAATTACCAGAAAGAAAGAA+AGG 0.528833 5_3:-5842592 None:intergenic
TACCAGAAAGAAAGAAAGGA+GGG 0.529239 5_3:-5842588 None:intergenic
CAAGCCATTGAAACCTATGA+AGG 0.539155 5_3:-5843800 None:intergenic
ATAACCCACAAAGATCTTGA+AGG 0.541294 5_3:-5843328 None:intergenic
TACTGTATCGAGTAAATCTA+GGG 0.549887 5_3:-5842434 None:intergenic
TCATAGGTTTCAATGGCTTG+AGG 0.551220 5_3:+5843803 Msa0794030:CDS
CCTCTTGTCAGGGCCTACAT+AGG 0.556427 5_3:-5844247 None:intergenic
AAAATCAAATACATCATGAA+AGG 0.558701 5_3:-5844209 None:intergenic
TTCTTCCAAGCCTCAAACCA+CGG 0.559243 5_3:-5843939 None:intergenic
TAGAGAAAGAGAGGATTGAG+CGG 0.561034 5_3:-5842513 None:intergenic
TAAGGCTATCCATTATACTC+GGG 0.565710 5_3:+5843911 Msa0794030:CDS
TGATCTATGGCTGAATGAGA+TGG 0.566426 5_3:+5843974 Msa0794030:CDS
TAACCCACAAAGATCTTGAA+GGG 0.577120 5_3:-5843327 None:intergenic
CTGTGGTCATCCTAAGAACA+AGG 0.579621 5_3:+5843737 Msa0794030:CDS
TATTCTTAGACCTCTTGTCA+GGG 0.580570 5_3:-5844257 None:intergenic
GGACTTGTTTGGAAAAGGAG+AGG 0.580718 5_3:-5842552 None:intergenic
TGATCCAACTACTTCACCTA+AGG 0.585896 5_3:+5843893 Msa0794030:CDS
TCACCATTACCAACTAGTAA+TGG 0.590252 5_3:+5843264 Msa0794030:CDS
ATAGCCTTAGGTGAAGTAGT+TGG 0.594477 5_3:-5843897 None:intergenic
TATAACCCTCCCTCCACTGT+CGG 0.604729 5_3:+5842475 Msa0794030:five_prime_UTR
CCTATGTAGGCCCTGACAAG+AGG 0.608691 5_3:+5844247 Msa0794030:three_prime_UTR
ACATGTTCACCGACAGTGGA+GGG 0.612323 5_3:-5842484 None:intergenic
ACGCACATGTTCACCGACAG+TGG 0.617473 5_3:-5842488 None:intergenic
CACATGTTCACCGACAGTGG+AGG 0.623716 5_3:-5842485 None:intergenic
GATGCTGTGAATTCACAGAC+TGG 0.623826 5_3:+5843771 Msa0794030:CDS
AAAGAGACTACAAAGATGGA+TGG 0.623848 5_3:+5843660 Msa0794030:CDS
GTTCACCGACAGTGGAGGGA+GGG 0.634569 5_3:-5842480 None:intergenic
TTACCAGAAAGAAAGAAAGG+AGG 0.643019 5_3:-5842589 None:intergenic
GCTATCCATTATACTCGGGG+AGG 0.652245 5_3:+5843915 Msa0794030:CDS
TGTTCACCGACAGTGGAGGG+AGG 0.653233 5_3:-5842481 None:intergenic
ACCAGAAAGAAAGAAAGGAG+GGG 0.656464 5_3:-5842587 None:intergenic
ACAGAGATGAGTTTGAGCAA+TGG 0.659058 5_3:+5843231 Msa0794030:intron
CTATCCATTATACTCGGGGA+GGG 0.668674 5_3:+5843916 Msa0794030:CDS
AAGGCTATCCATTATACTCG+GGG 0.680190 5_3:+5843912 Msa0794030:CDS
TGAAATGCAATGTCTTCACG+TGG 0.692922 5_3:-5843354 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTATATGATCAAA+TGG + chr5_3:5842975-5842994 Msa0794030:intron 10.0%
!!! TTTTTTTTATATGATCAAAT+GGG + chr5_3:5842976-5842995 Msa0794030:intron 10.0%
!!! TTTTTTTTTATATGATCAAA+TGG + chr5_3:5842975-5842994 Msa0794030:intron 10.0%
!!! TTTTTTTTATATGATCAAAT+GGG + chr5_3:5842976-5842995 Msa0794030:intron 10.0%
!!! TTTTTTGTATATGATCAAAT+GGG + chr5_3:5842769-5842788 Msa0794030:intron 15.0%
!! TTTGAATTATTTCAACAATT+GGG + chr5_3:5842809-5842828 Msa0794030:intron 15.0%
!!! TTTTTTCTATATGATCAAAT+GGG + chr5_3:5842870-5842889 Msa0794030:intron 15.0%
!! ATTTGAATTATTACAACAAT+TGG + chr5_3:5842910-5842929 Msa0794030:intron 15.0%
!! TTTGAATTATTACAACAATT+GGG + chr5_3:5842911-5842930 Msa0794030:intron 15.0%
!! AGAAAAAAAACAACAAATTA+AGG - chr5_3:5843210-5843229 None:intergenic 15.0%
!!! ATTATCAGATTTTTCTTTTT+TGG - chr5_3:5844018-5844037 None:intergenic 15.0%
!!! TTTTTTGTATATGATCAAAT+GGG + chr5_3:5842769-5842788 Msa0794030:intron 15.0%
!! TTTGAATTATTTCAACAATT+GGG + chr5_3:5842809-5842828 Msa0794030:intron 15.0%
!!! TTTTTTCTATATGATCAAAT+GGG + chr5_3:5842870-5842889 Msa0794030:intron 15.0%
!! ATTTGAATTATTACAACAAT+TGG + chr5_3:5842910-5842929 Msa0794030:intron 15.0%
!! TTTGAATTATTACAACAATT+GGG + chr5_3:5842911-5842930 Msa0794030:intron 15.0%
!! AGAAAAAAAACAACAAATTA+AGG - chr5_3:5843210-5843229 None:intergenic 15.0%
!!! ATTATCAGATTTTTCTTTTT+TGG - chr5_3:5844018-5844037 None:intergenic 15.0%
!!! TTTGTTTTCTTTGATGTATA+AGG + chr5_3:5842662-5842681 Msa0794030:intron 20.0%
!!! GTTTTTTGTATATGATCAAA+TGG + chr5_3:5842768-5842787 Msa0794030:intron 20.0%
!! CTTTGAATTATTTCAACAAT+TGG + chr5_3:5842808-5842827 Msa0794030:intron 20.0%
!!! GTTTTTTCTATATGATCAAA+TGG + chr5_3:5842869-5842888 Msa0794030:intron 20.0%
!! TATCACATTATTAAAACAGT+TGG + chr5_3:5843086-5843105 Msa0794030:intron 20.0%
!! AAAATCAAATACATCATGAA+AGG - chr5_3:5844212-5844231 None:intergenic 20.0%
!!! TTTGTTTTCTTTGATGTATA+AGG + chr5_3:5842662-5842681 Msa0794030:intron 20.0%
!!! GTTTTTTGTATATGATCAAA+TGG + chr5_3:5842768-5842787 Msa0794030:intron 20.0%
!! CTTTGAATTATTTCAACAAT+TGG + chr5_3:5842808-5842827 Msa0794030:intron 20.0%
!!! GTTTTTTCTATATGATCAAA+TGG + chr5_3:5842869-5842888 Msa0794030:intron 20.0%
!! TATCACATTATTAAAACAGT+TGG + chr5_3:5843086-5843105 Msa0794030:intron 20.0%
!! AAAATCAAATACATCATGAA+AGG - chr5_3:5844212-5844231 None:intergenic 20.0%
! ATATTCTATAATCCAGCTAT+TGG + chr5_3:5842356-5842375 Msa0794030:five_prime_UTR 25.0%
! TAATTACCAGAAAGAAAGAA+AGG - chr5_3:5842595-5842614 None:intergenic 25.0%
! TATCACATTATTACAACAAC+TGG + chr5_3:5842707-5842726 Msa0794030:intron 25.0%
! ATCACATTATTACAACAACT+GGG + chr5_3:5842708-5842727 Msa0794030:intron 25.0%
!! TTTCAACAATTGGGTTATTT+TGG + chr5_3:5842818-5842837 Msa0794030:intron 25.0%
!! TCTCTAAAAGAACAAATCAA+TGG - chr5_3:5843034-5843053 None:intergenic 25.0%
!!! TTTTTTCTGTCATGATCAAA+TGG + chr5_3:5843150-5843169 Msa0794030:intron 25.0%
!! TTTTTCTGTCATGATCAAAT+GGG + chr5_3:5843151-5843170 Msa0794030:intron 25.0%
! TCTCTATTTAGCTGATATTA+AGG + chr5_3:5843575-5843594 Msa0794030:CDS 25.0%
! TTTAGCTGATATTAAGGAAT+TGG + chr5_3:5843581-5843600 Msa0794030:CDS 25.0%
! ATTTGTTTAATGACCTATGT+AGG + chr5_3:5844234-5844253 Msa0794030:three_prime_UTR 25.0%
!!! ATTGAGTGTTTTAATCAACA+AGG + chr5_3:5844287-5844306 Msa0794030:three_prime_UTR 25.0%
! ATATTCTATAATCCAGCTAT+TGG + chr5_3:5842356-5842375 Msa0794030:five_prime_UTR 25.0%
! TAATTACCAGAAAGAAAGAA+AGG - chr5_3:5842595-5842614 None:intergenic 25.0%
! TATCACATTATTACAACAAC+TGG + chr5_3:5842707-5842726 Msa0794030:intron 25.0%
! ATCACATTATTACAACAACT+GGG + chr5_3:5842708-5842727 Msa0794030:intron 25.0%
!! TTTCAACAATTGGGTTATTT+TGG + chr5_3:5842818-5842837 Msa0794030:intron 25.0%
!! TCTCTAAAAGAACAAATCAA+TGG - chr5_3:5843034-5843053 None:intergenic 25.0%
!!! TTTTTTCTGTCATGATCAAA+TGG + chr5_3:5843150-5843169 Msa0794030:intron 25.0%
!! TTTTTCTGTCATGATCAAAT+GGG + chr5_3:5843151-5843170 Msa0794030:intron 25.0%
! TCTCTATTTAGCTGATATTA+AGG + chr5_3:5843575-5843594 Msa0794030:CDS 25.0%
! TTTAGCTGATATTAAGGAAT+TGG + chr5_3:5843581-5843600 Msa0794030:CDS 25.0%
! ATTTGTTTAATGACCTATGT+AGG + chr5_3:5844234-5844253 Msa0794030:three_prime_UTR 25.0%
!!! ATTGAGTGTTTTAATCAACA+AGG + chr5_3:5844287-5844306 Msa0794030:three_prime_UTR 25.0%
TATATTGCAATACCAATAGC+TGG - chr5_3:5842371-5842390 None:intergenic 30.0%
TACTGTATCGAGTAAATCTA+GGG - chr5_3:5842437-5842456 None:intergenic 30.0%
ATACTGTATCGAGTAAATCT+AGG - chr5_3:5842438-5842457 None:intergenic 30.0%
CTTTCACATTTCATTCGATT+GGG + chr5_3:5842624-5842643 Msa0794030:intron 30.0%
!! TTACCATTACTAGTTGGTAA+TGG - chr5_3:5843270-5843289 None:intergenic 30.0%
TTACCAACTAGTAATGGTAA+TGG + chr5_3:5843270-5843289 Msa0794030:CDS 30.0%
ATTCCATTACCATTACTAGT+TGG - chr5_3:5843276-5843295 None:intergenic 30.0%
TTAATTGGTATGATCTCAAC+AGG - chr5_3:5843417-5843436 None:intergenic 30.0%
TGAGAAAAAGTGGTCTTTAT+TGG + chr5_3:5843448-5843467 Msa0794030:CDS 30.0%
AAGATTCTTAACACCTAGTT+TGG + chr5_3:5843512-5843531 Msa0794030:CDS 30.0%
TTTGGCAAATTATCAAGGTT+GGG + chr5_3:5843530-5843549 Msa0794030:CDS 30.0%
ATTGAAGACCAATTCTTTCT+AGG - chr5_3:5843705-5843724 None:intergenic 30.0%
!! TAGAAAGAATTGGTCTTCAA+TGG + chr5_3:5843704-5843723 Msa0794030:CDS 30.0%
!!! TCAATGGTTTTGTATAACTG+TGG + chr5_3:5843720-5843739 Msa0794030:CDS 30.0%
TCAAGGAAATTCCAAACAAA+AGG - chr5_3:5843849-5843868 None:intergenic 30.0%
! TTTGTTTGGAATTTCCTTGA+AGG + chr5_3:5843849-5843868 Msa0794030:CDS 30.0%
ATTTCCTTGAAGGACATAAT+AGG + chr5_3:5843859-5843878 Msa0794030:CDS 30.0%
TTTCCTTGAAGGACATAATA+GGG + chr5_3:5843860-5843879 Msa0794030:CDS 30.0%
TAGAGATTTGAAGATGCATT+TGG + chr5_3:5844038-5844057 Msa0794030:exon 30.0%
! TGTTTAGAGTCAGTCAATTA+TGG + chr5_3:5844080-5844099 Msa0794030:three_prime_UTR 30.0%
ATGATGCAAGTTATGAGAAT+AGG + chr5_3:5844147-5844166 Msa0794030:three_prime_UTR 30.0%
TATATTGCAATACCAATAGC+TGG - chr5_3:5842371-5842390 None:intergenic 30.0%
TACTGTATCGAGTAAATCTA+GGG - chr5_3:5842437-5842456 None:intergenic 30.0%
ATACTGTATCGAGTAAATCT+AGG - chr5_3:5842438-5842457 None:intergenic 30.0%
CTTTCACATTTCATTCGATT+GGG + chr5_3:5842624-5842643 Msa0794030:intron 30.0%
!! TTACCATTACTAGTTGGTAA+TGG - chr5_3:5843270-5843289 None:intergenic 30.0%
TTACCAACTAGTAATGGTAA+TGG + chr5_3:5843270-5843289 Msa0794030:CDS 30.0%
ATTCCATTACCATTACTAGT+TGG - chr5_3:5843276-5843295 None:intergenic 30.0%
TTAATTGGTATGATCTCAAC+AGG - chr5_3:5843417-5843436 None:intergenic 30.0%
TGAGAAAAAGTGGTCTTTAT+TGG + chr5_3:5843448-5843467 Msa0794030:CDS 30.0%
AAGATTCTTAACACCTAGTT+TGG + chr5_3:5843512-5843531 Msa0794030:CDS 30.0%
TTTGGCAAATTATCAAGGTT+GGG + chr5_3:5843530-5843549 Msa0794030:CDS 30.0%
ATTGAAGACCAATTCTTTCT+AGG - chr5_3:5843705-5843724 None:intergenic 30.0%
!! TAGAAAGAATTGGTCTTCAA+TGG + chr5_3:5843704-5843723 Msa0794030:CDS 30.0%
!!! TCAATGGTTTTGTATAACTG+TGG + chr5_3:5843720-5843739 Msa0794030:CDS 30.0%
TCAAGGAAATTCCAAACAAA+AGG - chr5_3:5843849-5843868 None:intergenic 30.0%
! TTTGTTTGGAATTTCCTTGA+AGG + chr5_3:5843849-5843868 Msa0794030:CDS 30.0%
ATTTCCTTGAAGGACATAAT+AGG + chr5_3:5843859-5843878 Msa0794030:CDS 30.0%
TTTCCTTGAAGGACATAATA+GGG + chr5_3:5843860-5843879 Msa0794030:CDS 30.0%
TAGAGATTTGAAGATGCATT+TGG + chr5_3:5844038-5844057 Msa0794030:exon 30.0%
! TGTTTAGAGTCAGTCAATTA+TGG + chr5_3:5844080-5844099 Msa0794030:three_prime_UTR 30.0%
ATGATGCAAGTTATGAGAAT+AGG + chr5_3:5844147-5844166 Msa0794030:three_prime_UTR 30.0%
GAAGAGAAATAGAGAAAGAG+AGG - chr5_3:5842525-5842544 None:intergenic 35.0%
! TTGTTGGACTTGTTTGGAAA+AGG - chr5_3:5842560-5842579 None:intergenic 35.0%
TACCAGAAAGAAAGAAAGGA+GGG - chr5_3:5842591-5842610 None:intergenic 35.0%
TTACCAGAAAGAAAGAAAGG+AGG - chr5_3:5842592-5842611 None:intergenic 35.0%
CCAATCGAATGAAATGTGAA+AGG - chr5_3:5842626-5842645 None:intergenic 35.0%
CCTTTCACATTTCATTCGAT+TGG + chr5_3:5842623-5842642 Msa0794030:intron 35.0%
!! TACTAGTTGGTAATGGTGAT+TGG - chr5_3:5843263-5843282 None:intergenic 35.0%
TCACCATTACCAACTAGTAA+TGG + chr5_3:5843264-5843283 Msa0794030:CDS 35.0%
AAACCCTTCAAGATCTTTGT+GGG + chr5_3:5843324-5843343 Msa0794030:CDS 35.0%
TAACCCACAAAGATCTTGAA+GGG - chr5_3:5843330-5843349 None:intergenic 35.0%
ATAACCCACAAAGATCTTGA+AGG - chr5_3:5843331-5843350 None:intergenic 35.0%
GTCGTCATTCAATCATGAAA+AGG + chr5_3:5843382-5843401 Msa0794030:CDS 35.0%
CTCAGATCTGATTGCTTAAT+TGG - chr5_3:5843432-5843451 None:intergenic 35.0%
CAATCAGATCTGAGAAAAAG+TGG + chr5_3:5843438-5843457 Msa0794030:CDS 35.0%
! CCTTGATAATTTGCCAAACT+AGG - chr5_3:5843528-5843547 None:intergenic 35.0%
CCTAGTTTGGCAAATTATCA+AGG + chr5_3:5843525-5843544 Msa0794030:CDS 35.0%
GTTTGGCAAATTATCAAGGT+TGG + chr5_3:5843529-5843548 Msa0794030:CDS 35.0%
GGAATTGGTAGATTTGATTG+AGG + chr5_3:5843596-5843615 Msa0794030:CDS 35.0%
TCCAAAAGAGACTACAAAGA+TGG + chr5_3:5843656-5843675 Msa0794030:CDS 35.0%
! TCCATCTTTGTAGTCTCTTT+TGG - chr5_3:5843660-5843679 None:intergenic 35.0%
AAAGAGACTACAAAGATGGA+TGG + chr5_3:5843660-5843679 Msa0794030:CDS 35.0%
CTGTGTATCCTAGAAAGAAT+TGG + chr5_3:5843694-5843713 Msa0794030:CDS 35.0%
CTTTCCTTCATAGGTTTCAA+TGG + chr5_3:5843796-5843815 Msa0794030:CDS 35.0%
!!! TTGGTTCTGTACCTTTTGTT+TGG + chr5_3:5843835-5843854 Msa0794030:CDS 35.0%
CTAAGGCTATCCATTATACT+CGG + chr5_3:5843910-5843929 Msa0794030:CDS 35.0%
TAAGGCTATCCATTATACTC+GGG + chr5_3:5843911-5843930 Msa0794030:CDS 35.0%
!! ATTGCGAATTTGCTGATCTA+TGG + chr5_3:5843961-5843980 Msa0794030:CDS 35.0%
!!! TTTCTTTTTTGGCTTGAGCA+AGG - chr5_3:5844007-5844026 None:intergenic 35.0%
TATTCTTAGACCTCTTGTCA+GGG - chr5_3:5844260-5844279 None:intergenic 35.0%
TTATTCTTAGACCTCTTGTC+AGG - chr5_3:5844261-5844280 None:intergenic 35.0%
TTGCGATTATGCATTGTTCT+TGG + chr5_3:5844318-5844337 Msa0794030:three_prime_UTR 35.0%
! AGCTTCAATATAGTTTGTGC+AGG + chr5_3:5844363-5844382 Msa0794030:three_prime_UTR 35.0%
! GCTTCAATATAGTTTGTGCA+GGG + chr5_3:5844364-5844383 Msa0794030:three_prime_UTR 35.0%
! CTTCAATATAGTTTGTGCAG+GGG + chr5_3:5844365-5844384 Msa0794030:three_prime_UTR 35.0%
GAAGAGAAATAGAGAAAGAG+AGG - chr5_3:5842525-5842544 None:intergenic 35.0%
! TTGTTGGACTTGTTTGGAAA+AGG - chr5_3:5842560-5842579 None:intergenic 35.0%
TACCAGAAAGAAAGAAAGGA+GGG - chr5_3:5842591-5842610 None:intergenic 35.0%
TTACCAGAAAGAAAGAAAGG+AGG - chr5_3:5842592-5842611 None:intergenic 35.0%
CCAATCGAATGAAATGTGAA+AGG - chr5_3:5842626-5842645 None:intergenic 35.0%
CCTTTCACATTTCATTCGAT+TGG + chr5_3:5842623-5842642 Msa0794030:intron 35.0%
!! TACTAGTTGGTAATGGTGAT+TGG - chr5_3:5843263-5843282 None:intergenic 35.0%
TCACCATTACCAACTAGTAA+TGG + chr5_3:5843264-5843283 Msa0794030:CDS 35.0%
AAACCCTTCAAGATCTTTGT+GGG + chr5_3:5843324-5843343 Msa0794030:CDS 35.0%
TAACCCACAAAGATCTTGAA+GGG - chr5_3:5843330-5843349 None:intergenic 35.0%
ATAACCCACAAAGATCTTGA+AGG - chr5_3:5843331-5843350 None:intergenic 35.0%
GTCGTCATTCAATCATGAAA+AGG + chr5_3:5843382-5843401 Msa0794030:CDS 35.0%
CTCAGATCTGATTGCTTAAT+TGG - chr5_3:5843432-5843451 None:intergenic 35.0%
CAATCAGATCTGAGAAAAAG+TGG + chr5_3:5843438-5843457 Msa0794030:CDS 35.0%
! CCTTGATAATTTGCCAAACT+AGG - chr5_3:5843528-5843547 None:intergenic 35.0%
CCTAGTTTGGCAAATTATCA+AGG + chr5_3:5843525-5843544 Msa0794030:CDS 35.0%
GTTTGGCAAATTATCAAGGT+TGG + chr5_3:5843529-5843548 Msa0794030:CDS 35.0%
GGAATTGGTAGATTTGATTG+AGG + chr5_3:5843596-5843615 Msa0794030:CDS 35.0%
TCCAAAAGAGACTACAAAGA+TGG + chr5_3:5843656-5843675 Msa0794030:CDS 35.0%
! TCCATCTTTGTAGTCTCTTT+TGG - chr5_3:5843660-5843679 None:intergenic 35.0%
AAAGAGACTACAAAGATGGA+TGG + chr5_3:5843660-5843679 Msa0794030:CDS 35.0%
CTGTGTATCCTAGAAAGAAT+TGG + chr5_3:5843694-5843713 Msa0794030:CDS 35.0%
CTTTCCTTCATAGGTTTCAA+TGG + chr5_3:5843796-5843815 Msa0794030:CDS 35.0%
!!! TTGGTTCTGTACCTTTTGTT+TGG + chr5_3:5843835-5843854 Msa0794030:CDS 35.0%
CTAAGGCTATCCATTATACT+CGG + chr5_3:5843910-5843929 Msa0794030:CDS 35.0%
TAAGGCTATCCATTATACTC+GGG + chr5_3:5843911-5843930 Msa0794030:CDS 35.0%
!! ATTGCGAATTTGCTGATCTA+TGG + chr5_3:5843961-5843980 Msa0794030:CDS 35.0%
!!! TTTCTTTTTTGGCTTGAGCA+AGG - chr5_3:5844007-5844026 None:intergenic 35.0%
TATTCTTAGACCTCTTGTCA+GGG - chr5_3:5844260-5844279 None:intergenic 35.0%
TTATTCTTAGACCTCTTGTC+AGG - chr5_3:5844261-5844280 None:intergenic 35.0%
TTGCGATTATGCATTGTTCT+TGG + chr5_3:5844318-5844337 Msa0794030:three_prime_UTR 35.0%
TAGAGAAAGAGAGGATTGAG+CGG - chr5_3:5842516-5842535 None:intergenic 40.0%
! CAAGTGTTGTTGGACTTGTT+TGG - chr5_3:5842566-5842585 None:intergenic 40.0%
ACCAGAAAGAAAGAAAGGAG+GGG - chr5_3:5842590-5842609 None:intergenic 40.0%
ACAGAGATGAGTTTGAGCAA+TGG + chr5_3:5843231-5843250 Msa0794030:intron 40.0%
GAAACCCTTCAAGATCTTTG+TGG + chr5_3:5843323-5843342 Msa0794030:CDS 40.0%
TGAAATGCAATGTCTTCACG+TGG - chr5_3:5843357-5843376 None:intergenic 40.0%
ACGTGAAGACATTGCATTTC+AGG + chr5_3:5843356-5843375 Msa0794030:CDS 40.0%
GGAGTAAGAACCTTGTTCTT+AGG - chr5_3:5843750-5843769 None:intergenic 40.0%
CAAGCCATTGAAACCTATGA+AGG - chr5_3:5843803-5843822 None:intergenic 40.0%
TCATAGGTTTCAATGGCTTG+AGG + chr5_3:5843803-5843822 Msa0794030:CDS 40.0%
TGGCTTGAGGATGATGAAAT+TGG + chr5_3:5843816-5843835 Msa0794030:CDS 40.0%
TGATCCAACTACTTCACCTA+AGG + chr5_3:5843893-5843912 Msa0794030:CDS 40.0%
! ATAGCCTTAGGTGAAGTAGT+TGG - chr5_3:5843900-5843919 None:intergenic 40.0%
!! CGAGTATAATGGATAGCCTT+AGG - chr5_3:5843912-5843931 None:intergenic 40.0%
AAGGCTATCCATTATACTCG+GGG + chr5_3:5843912-5843931 Msa0794030:CDS 40.0%
TGATCTATGGCTGAATGAGA+TGG + chr5_3:5843974-5843993 Msa0794030:CDS 40.0%
TAGAGAAAGAGAGGATTGAG+CGG - chr5_3:5842516-5842535 None:intergenic 40.0%
! CAAGTGTTGTTGGACTTGTT+TGG - chr5_3:5842566-5842585 None:intergenic 40.0%
ACCAGAAAGAAAGAAAGGAG+GGG - chr5_3:5842590-5842609 None:intergenic 40.0%
ACAGAGATGAGTTTGAGCAA+TGG + chr5_3:5843231-5843250 Msa0794030:intron 40.0%
GAAACCCTTCAAGATCTTTG+TGG + chr5_3:5843323-5843342 Msa0794030:CDS 40.0%
TGAAATGCAATGTCTTCACG+TGG - chr5_3:5843357-5843376 None:intergenic 40.0%
ACGTGAAGACATTGCATTTC+AGG + chr5_3:5843356-5843375 Msa0794030:CDS 40.0%
GGAGTAAGAACCTTGTTCTT+AGG - chr5_3:5843750-5843769 None:intergenic 40.0%
CAAGCCATTGAAACCTATGA+AGG - chr5_3:5843803-5843822 None:intergenic 40.0%
TCATAGGTTTCAATGGCTTG+AGG + chr5_3:5843803-5843822 Msa0794030:CDS 40.0%
TGGCTTGAGGATGATGAAAT+TGG + chr5_3:5843816-5843835 Msa0794030:CDS 40.0%
TGATCCAACTACTTCACCTA+AGG + chr5_3:5843893-5843912 Msa0794030:CDS 40.0%
! ATAGCCTTAGGTGAAGTAGT+TGG - chr5_3:5843900-5843919 None:intergenic 40.0%
!! CGAGTATAATGGATAGCCTT+AGG - chr5_3:5843912-5843931 None:intergenic 40.0%
AAGGCTATCCATTATACTCG+GGG + chr5_3:5843912-5843931 Msa0794030:CDS 40.0%
TGATCTATGGCTGAATGAGA+TGG + chr5_3:5843974-5843993 Msa0794030:CDS 40.0%
! GGACTTGTTTGGAAAAGGAG+AGG - chr5_3:5842555-5842574 None:intergenic 45.0%
TCCCCTCCTTTCTTTCTTTC+TGG + chr5_3:5842586-5842605 Msa0794030:five_prime_UTR 45.0%
GGTCTTTATTGGCGTGAAAG+AGG + chr5_3:5843459-5843478 Msa0794030:CDS 45.0%
TCAAGGTTGGGCTATGTTTG+TGG + chr5_3:5843542-5843561 Msa0794030:CDS 45.0%
CTGTGGTCATCCTAAGAACA+AGG + chr5_3:5843737-5843756 Msa0794030:CDS 45.0%
GTCTGTGAATTCACAGCATC+AGG - chr5_3:5843771-5843790 None:intergenic 45.0%
GATGCTGTGAATTCACAGAC+TGG + chr5_3:5843771-5843790 Msa0794030:CDS 45.0%
AGACTGGTGCTTTCCTTCAT+AGG + chr5_3:5843787-5843806 Msa0794030:CDS 45.0%
CAGCCCTATTATGTCCTTCA+AGG - chr5_3:5843866-5843885 None:intergenic 45.0%
CTATCCATTATACTCGGGGA+GGG + chr5_3:5843916-5843935 Msa0794030:CDS 45.0%
TTCTTCCAAGCCTCAAACCA+CGG - chr5_3:5843942-5843961 None:intergenic 45.0%
! GGACTTGTTTGGAAAAGGAG+AGG - chr5_3:5842555-5842574 None:intergenic 45.0%
TCCCCTCCTTTCTTTCTTTC+TGG + chr5_3:5842586-5842605 Msa0794030:five_prime_UTR 45.0%
GGTCTTTATTGGCGTGAAAG+AGG + chr5_3:5843459-5843478 Msa0794030:CDS 45.0%
TCAAGGTTGGGCTATGTTTG+TGG + chr5_3:5843542-5843561 Msa0794030:CDS 45.0%
CTGTGGTCATCCTAAGAACA+AGG + chr5_3:5843737-5843756 Msa0794030:CDS 45.0%
GTCTGTGAATTCACAGCATC+AGG - chr5_3:5843771-5843790 None:intergenic 45.0%
GATGCTGTGAATTCACAGAC+TGG + chr5_3:5843771-5843790 Msa0794030:CDS 45.0%
AGACTGGTGCTTTCCTTCAT+AGG + chr5_3:5843787-5843806 Msa0794030:CDS 45.0%
CAGCCCTATTATGTCCTTCA+AGG - chr5_3:5843866-5843885 None:intergenic 45.0%
CTATCCATTATACTCGGGGA+GGG + chr5_3:5843916-5843935 Msa0794030:CDS 45.0%
TTCTTCCAAGCCTCAAACCA+CGG - chr5_3:5843942-5843961 None:intergenic 45.0%
TATAACCCTCCCTCCACTGT+CGG + chr5_3:5842475-5842494 Msa0794030:five_prime_UTR 50.0%
ACATGTTCACCGACAGTGGA+GGG - chr5_3:5842487-5842506 None:intergenic 50.0%
! AAGGAGGGGACAAGTGTTGT+TGG - chr5_3:5842576-5842595 None:intergenic 50.0%
GCTATCCATTATACTCGGGG+AGG + chr5_3:5843915-5843934 Msa0794030:CDS 50.0%
TATAACCCTCCCTCCACTGT+CGG + chr5_3:5842475-5842494 Msa0794030:five_prime_UTR 50.0%
ACATGTTCACCGACAGTGGA+GGG - chr5_3:5842487-5842506 None:intergenic 50.0%
! AAGGAGGGGACAAGTGTTGT+TGG - chr5_3:5842576-5842595 None:intergenic 50.0%
GCTATCCATTATACTCGGGG+AGG + chr5_3:5843915-5843934 Msa0794030:CDS 50.0%
CACATGTTCACCGACAGTGG+AGG - chr5_3:5842488-5842507 None:intergenic 55.0%
ACGCACATGTTCACCGACAG+TGG - chr5_3:5842491-5842510 None:intergenic 55.0%
CCTCTTGTCAGGGCCTACAT+AGG - chr5_3:5844250-5844269 None:intergenic 55.0%
CCTATGTAGGCCCTGACAAG+AGG + chr5_3:5844247-5844266 Msa0794030:three_prime_UTR 55.0%
CACATGTTCACCGACAGTGG+AGG - chr5_3:5842488-5842507 None:intergenic 55.0%
ACGCACATGTTCACCGACAG+TGG - chr5_3:5842491-5842510 None:intergenic 55.0%
CCTCTTGTCAGGGCCTACAT+AGG - chr5_3:5844250-5844269 None:intergenic 55.0%
CCTATGTAGGCCCTGACAAG+AGG + chr5_3:5844247-5844266 Msa0794030:three_prime_UTR 55.0%
GTTCACCGACAGTGGAGGGA+GGG - chr5_3:5842483-5842502 None:intergenic 60.0%
TGTTCACCGACAGTGGAGGG+AGG - chr5_3:5842484-5842503 None:intergenic 60.0%
ACGGCCCTCCCCGAGTATAA+TGG - chr5_3:5843923-5843942 None:intergenic 60.0%
ATTATACTCGGGGAGGGCCG+TGG + chr5_3:5843922-5843941 Msa0794030:CDS 60.0%
GTTCACCGACAGTGGAGGGA+GGG - chr5_3:5842483-5842502 None:intergenic 60.0%
TGTTCACCGACAGTGGAGGG+AGG - chr5_3:5842484-5842503 None:intergenic 60.0%
ACGGCCCTCCCCGAGTATAA+TGG - chr5_3:5843923-5843942 None:intergenic 60.0%
ATTATACTCGGGGAGGGCCG+TGG + chr5_3:5843922-5843941 Msa0794030:CDS 60.0%
! GAGGGCCGTGGTTTGAGGCT+TGG + chr5_3:5843934-5843953 Msa0794030:CDS 65.0%
! GAGGGCCGTGGTTTGAGGCT+TGG + chr5_3:5843934-5843953 Msa0794030:CDS 65.0%
TCGGGGAGGGCCGTGGTTTG+AGG + chr5_3:5843929-5843948 Msa0794030:CDS 70.0%
TCGGGGAGGGCCGTGGTTTG+AGG + chr5_3:5843929-5843948 Msa0794030:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 5842344 5844385 5842344 ID=Msa0794030;Name=Msa0794030
chr5_3 mRNA 5842344 5844385 5842344 ID=Msa0794030-mRNA-1;Parent=Msa0794030;Name=Msa0794030-mRNA-1;_AED=0.00;_eAED=0.00;_QI=264|0|0.5|1|0|0.5|2|345|267
chr5_3 exon 5842344 5842607 5842344 ID=Msa0794030-mRNA-1:exon:2328;Parent=Msa0794030-mRNA-1
chr5_3 exon 5843237 5844385 5843237 ID=Msa0794030-mRNA-1:exon:2329;Parent=Msa0794030-mRNA-1
chr5_3 five_prime_UTR 5842344 5842607 5842344 ID=Msa0794030-mRNA-1:five_prime_utr;Parent=Msa0794030-mRNA-1
chr5_3 CDS 5843237 5844040 5843237 ID=Msa0794030-mRNA-1:cds;Parent=Msa0794030-mRNA-1
chr5_3 three_prime_UTR 5844041 5844385 5844041 ID=Msa0794030-mRNA-1:three_prime_utr;Parent=Msa0794030-mRNA-1
Gene Sequence

>Msa0794030

ATGAGTTTGAGCAATGGTAAAAACCAATCACCATTACCAACTAGTAATGGTAATGGAATAGATTCTGCTGAGAATGAGAATGCAAAGAAACCCTTCAAGATCTTTGTGGGTTATGATCCACGTGAAGACATTGCATTTCAGGTATGTCGTCATTCAATCATGAAAAGGTCTTCAATTCCTGTTGAGATCATACCAATTAAGCAATCAGATCTGAGAAAAAGTGGTCTTTATTGGCGTGAAAGAGGTCAATTTGAGAGCACTGAGTTTTCTTTTACAAGATTCTTAACACCTAGTTTGGCAAATTATCAAGGTTGGGCTATGTTTGTGGATTGTGATTTTCTCTATTTAGCTGATATTAAGGAATTGGTAGATTTGATTGAGGATAAGTATGCTATTATGTGTGTTCAACATGATTATACTCCAAAAGAGACTACAAAGATGGATGGTGCTGTTCAAACTGTGTATCCTAGAAAGAATTGGTCTTCAATGGTTTTGTATAACTGTGGTCATCCTAAGAACAAGGTTCTTACTCCTGATGCTGTGAATTCACAGACTGGTGCTTTCCTTCATAGGTTTCAATGGCTTGAGGATGATGAAATTGGTTCTGTACCTTTTGTTTGGAATTTCCTTGAAGGACATAATAGGGCTGTTGAGAATGATCCAACTACTTCACCTAAGGCTATCCATTATACTCGGGGAGGGCCGTGGTTTGAGGCTTGGAAGAATTGCGAATTTGCTGATCTATGGCTGAATGAGATGGAAGAGTACCTTGCTCAAGCCAAAAAAGAAAAATCTGATAATTAG
Protein sequence

>Msa0794030

MSLSNGKNQSPLPTSNGNGIDSAENENAKKPFKIFVGYDPREDIAFQVCRHSIMKRSSIPVEIIPIKQSDLRKSGLYWRERGQFESTEFSFTRFLTPSLANYQGWAMFVDCDFLYLADIKELVDLIEDKYAIMCVQHDYTPKETTKMDGAVQTVYPRKNWSSMVLYNCGHPKNKVLTPDAVNSQTGAFLHRFQWLEDDEIGSVPFVWNFLEGHNRAVENDPTTSPKAIHYTRGGPWFEAWKNCEFADLWLNEMEEYLAQAKKEKSDN*