AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1132330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1132330 MtrunA17_Chr5g0395061 95.131 267 7 1 1 267 1 261 0.0 532
Msa1132330 MtrunA17_Chr6g0468311 75.000 48 6 1 1 48 1 42 5.31e-18 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1132330 AT1G64980.2 78.277 267 55 2 3 267 12 277 3.68e-163 453
Msa1132330 AT1G64980.1 79.401 267 45 3 1 267 1 257 1.65e-162 451
Msa1132330 AT1G64980.3 79.401 267 45 3 1 267 1 257 1.65e-162 451

Find 91 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCCTCCTTTCTTTCTTTC+TGG 0.032183 7_3:-85197405 Msa1132330:intron
CTCAGATCTGATTGCTTAAT+TGG 0.085095 7_3:+85196562 None:intergenic
TCCATCTTTGTAGTCTCTTT+TGG 0.193307 7_3:+85196334 None:intergenic
ATTGAAGACCAATTCTTTCT+AGG 0.195136 7_3:+85196289 None:intergenic
TCTCTATTTAGCTGATATTA+AGG 0.242104 7_3:-85196416 Msa1132330:CDS
ACGTGAAGACATTGCATTTC+AGG 0.259904 7_3:-85196635 Msa1132330:CDS
ATTTCCTTGAAGGACATAAT+AGG 0.275347 7_3:-85196132 Msa1132330:CDS
AAGATTCTTAACACCTAGTT+TGG 0.296665 7_3:-85196479 Msa1132330:CDS
TGTTTAGAGTCAGTCAATTA+TGG 0.301013 7_3:-85195911 Msa1132330:three_prime_UTR
TGGCTTGAGGATGATGAAAT+TGG 0.336305 7_3:-85196175 Msa1132330:CDS
GGAGTAAGAACCTTGTTCTT+AGG 0.346332 7_3:+85196244 None:intergenic
TTTAGCTGATATTAAGGAAT+TGG 0.346940 7_3:-85196410 Msa1132330:CDS
TAGAGATTTGAAGATGCATT+TGG 0.347816 7_3:-85195953 Msa1132330:three_prime_UTR
TTGCGATTATGCATTGTTCT+TGG 0.347846 7_3:-85195666 Msa1132330:three_prime_UTR
CTTTCCTTCATAGGTTTCAA+TGG 0.348106 7_3:-85196195 Msa1132330:CDS
GAGGGCCGTGGTTTGAGGCT+TGG 0.352682 7_3:-85196057 Msa1132330:CDS
AGACTGGTGCTTTCCTTCAT+AGG 0.356440 7_3:-85196204 Msa1132330:CDS
TGTGATTGATTTGAAGCTAT+TGG 0.360642 7_3:+85197582 None:intergenic
TCGGGGAGGGCCGTGGTTTG+AGG 0.366626 7_3:-85196062 Msa1132330:CDS
ATACTGTATCGAGTAAATCT+AGG 0.369673 7_3:+85197556 None:intergenic
ACGGCCCTCCCCGAGTATAA+TGG 0.377630 7_3:+85196071 None:intergenic
TTATTCTTAGACCTCTTGTC+AGG 0.383421 7_3:+85195726 None:intergenic
ATTGCGAATTTGCTGATCTA+TGG 0.387264 7_3:-85196030 Msa1132330:CDS
TTTCCTTGAAGGACATAATA+GGG 0.389168 7_3:-85196131 Msa1132330:CDS
TACTAGTTGGTAATGGTGAT+TGG 0.390769 7_3:+85196731 None:intergenic
TAGAAAGAATTGGTCTTCAA+TGG 0.393256 7_3:-85196287 Msa1132330:CDS
CAGCCCTATTATGTCCTTCA+AGG 0.399988 7_3:+85196128 None:intergenic
TTAATTGGTATGATCTCAAC+AGG 0.402966 7_3:+85196577 None:intergenic
TCAAGGTTGGGCTATGTTTG+TGG 0.418527 7_3:-85196449 Msa1132330:CDS
ATTTGTTTAATGACCTATGT+AGG 0.427955 7_3:-85195750 Msa1132330:three_prime_UTR
CCTAGTTTGGCAAATTATCA+AGG 0.430049 7_3:-85196466 Msa1132330:CDS
CCTTGATAATTTGCCAAACT+AGG 0.430154 7_3:+85196466 None:intergenic
ATATTCTATAATCCAGCTAT+TGG 0.437101 7_3:-85197643 Msa1132330:five_prime_UTR
TTTGGCAAATTATCAAGGTT+GGG 0.439482 7_3:-85196461 Msa1132330:CDS
AAACCCTTCAAGATCTTTGT+GGG 0.439682 7_3:-85196667 Msa1132330:CDS
CAAGTGTTGTTGGACTTGTT+TGG 0.442730 7_3:+85197428 None:intergenic
GTCTGTGAATTCACAGCATC+AGG 0.443576 7_3:+85196223 None:intergenic
CTGTGTATCCTAGAAAGAAT+TGG 0.452193 7_3:-85196297 Msa1132330:CDS
TTTGTTTGGAATTTCCTTGA+AGG 0.455406 7_3:-85196142 Msa1132330:CDS
CGAGTATAATGGATAGCCTT+AGG 0.455563 7_3:+85196082 None:intergenic
GAAGAGAAATAGAGAAAGAG+AGG 0.456795 7_3:+85197469 None:intergenic
GTTTGGCAAATTATCAAGGT+TGG 0.457948 7_3:-85196462 Msa1132330:CDS
AAGGAGGGGACAAGTGTTGT+TGG 0.460012 7_3:+85197418 None:intergenic
AGCTTCAATATAGTTTGTGC+AGG 0.461318 7_3:-85195621 Msa1132330:three_prime_UTR
GAAACCCTTCAAGATCTTTG+TGG 0.462717 7_3:-85196668 Msa1132330:CDS
ATGATGCAAGTTATGAGAAT+AGG 0.469528 7_3:-85195837 Msa1132330:three_prime_UTR
ATTCCATTACCATTACTAGT+TGG 0.470434 7_3:+85196718 None:intergenic
TCAAGGAAATTCCAAACAAA+AGG 0.473734 7_3:+85196145 None:intergenic
ATTATACTCGGGGAGGGCCG+TGG 0.478358 7_3:-85196069 Msa1132330:CDS
CTAAGGCTATCCATTATACT+CGG 0.478464 7_3:-85196081 Msa1132330:CDS
GTCGTCATTCAATCATGAAA+AGG 0.493012 7_3:-85196609 Msa1132330:CDS
TTACCATTACTAGTTGGTAA+TGG 0.495217 7_3:+85196724 None:intergenic
TATATTGCAATACCAATAGC+TGG 0.497053 7_3:+85197631 None:intergenic
TCCAAAAGAGACTACAAAGA+TGG 0.508455 7_3:-85196335 Msa1132330:CDS
GGAATTGGTAGATTTGATTG+AGG 0.511326 7_3:-85196395 Msa1132330:CDS
TTACCAACTAGTAATGGTAA+TGG 0.521531 7_3:-85196721 Msa1132330:CDS
GGTCTTTATTGGCGTGAAAG+AGG 0.522858 7_3:-85196532 Msa1132330:CDS
TAATTACCAGAAAGAAAGAA+AGG 0.528833 7_3:+85197399 None:intergenic
TACCAGAAAGAAAGAAAGGA+GGG 0.529239 7_3:+85197403 None:intergenic
CAAGCCATTGAAACCTATGA+AGG 0.539155 7_3:+85196191 None:intergenic
ATAACCCACAAAGATCTTGA+AGG 0.541294 7_3:+85196663 None:intergenic
TACTGTATCGAGTAAATCTA+GGG 0.549887 7_3:+85197557 None:intergenic
TCATAGGTTTCAATGGCTTG+AGG 0.551220 7_3:-85196188 Msa1132330:CDS
CCTCTTGTCAGGGCCTACAT+AGG 0.556427 7_3:+85195737 None:intergenic
AAAATCAAATACATCATGAA+AGG 0.558701 7_3:+85195775 None:intergenic
TTCTTCCAAGCCTCAAACCA+CGG 0.559243 7_3:+85196052 None:intergenic
TAGAGAAAGAGAGGATTGAG+CGG 0.561034 7_3:+85197478 None:intergenic
TAAGGCTATCCATTATACTC+GGG 0.565710 7_3:-85196080 Msa1132330:CDS
TGATCTATGGCTGAATGAGA+TGG 0.566426 7_3:-85196017 Msa1132330:CDS
TATTCTTAGACCTCTTGTCA+GGG 0.567090 7_3:+85195727 None:intergenic
TAACCCACAAAGATCTTGAA+GGG 0.577120 7_3:+85196664 None:intergenic
CTGTGGTCATCCTAAGAACA+AGG 0.579621 7_3:-85196254 Msa1132330:CDS
TGATCCAACTACTTCACCTA+AGG 0.585896 7_3:-85196098 Msa1132330:CDS
TCACCATTACCAACTAGTAA+TGG 0.590252 7_3:-85196727 Msa1132330:CDS
ATAGCCTTAGGTGAAGTAGT+TGG 0.594477 7_3:+85196094 None:intergenic
TATAACCCTCCCTCCACTGT+CGG 0.604729 7_3:-85197516 Msa1132330:five_prime_UTR
CCTATGTAGGCCCTGACAAG+AGG 0.608691 7_3:-85195737 Msa1132330:three_prime_UTR
ACATGTTCACCGACAGTGGA+GGG 0.612323 7_3:+85197507 None:intergenic
ACGCACATGTTCACCGACAG+TGG 0.617473 7_3:+85197503 None:intergenic
CACATGTTCACCGACAGTGG+AGG 0.623716 7_3:+85197506 None:intergenic
GATGCTGTGAATTCACAGAC+TGG 0.623826 7_3:-85196220 Msa1132330:CDS
AAAGAGACTACAAAGATGGA+TGG 0.623848 7_3:-85196331 Msa1132330:CDS
GTTCACCGACAGTGGAGGGA+GGG 0.634569 7_3:+85197511 None:intergenic
TTACCAGAAAGAAAGAAAGG+AGG 0.643019 7_3:+85197402 None:intergenic
GCTATCCATTATACTCGGGG+AGG 0.652245 7_3:-85196076 Msa1132330:CDS
TGTTCACCGACAGTGGAGGG+AGG 0.653233 7_3:+85197510 None:intergenic
ACCAGAAAGAAAGAAAGGAG+GGG 0.656464 7_3:+85197404 None:intergenic
ACAGAGATGAGTTTGAGCAA+TGG 0.659058 7_3:-85196760 Msa1132330:intron
CTATCCATTATACTCGGGGA+GGG 0.668674 7_3:-85196075 Msa1132330:CDS
AAGGCTATCCATTATACTCG+GGG 0.680190 7_3:-85196079 Msa1132330:CDS
TGAAATGCAATGTCTTCACG+TGG 0.692922 7_3:+85196637 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTATATGATCAAAT+GGG - chr7_3:85196260-85196279 Msa1132330:CDS 10.0%
!!! TTTTTTTTATATGATCAAAT+GGG - chr7_3:85196260-85196279 Msa1132330:CDS 10.0%
!!! TTTTTTGTATATGATCAAAT+GGG - chr7_3:85196054-85196073 Msa1132330:CDS 15.0%
!! TTTGAATTATTACAACAATT+GGG - chr7_3:85196094-85196113 Msa1132330:CDS 15.0%
!!! TTTTTTCTATATGATCAAAT+GGG - chr7_3:85196155-85196174 Msa1132330:CDS 15.0%
!! ATTTGAATTATTACAACAAT+TGG - chr7_3:85196195-85196214 Msa1132330:CDS 15.0%
!! TTTGAATTATTACAACAATT+GGG - chr7_3:85196094-85196113 Msa1132330:CDS 15.0%
!!! GTTTTTTTTATATGATCAAA+TGG - chr7_3:85196259-85196278 Msa1132330:CDS 15.0%
!! GAAAAAAAAACAACAAATTA+AGG + chr7_3:85196494-85196513 None:intergenic 15.0%
!!! ATTATCAGATTTTTCTTTTT+TGG + chr7_3:85197303-85197322 None:intergenic 15.0%
!!! TTTTTTGTATATGATCAAAT+GGG - chr7_3:85196054-85196073 Msa1132330:CDS 15.0%
!! TTTGAATTATTACAACAATT+GGG - chr7_3:85196094-85196113 Msa1132330:CDS 15.0%
!!! TTTTTTCTATATGATCAAAT+GGG - chr7_3:85196155-85196174 Msa1132330:CDS 15.0%
!! ATTTGAATTATTACAACAAT+TGG - chr7_3:85196195-85196214 Msa1132330:CDS 15.0%
!! TTTGAATTATTACAACAATT+GGG - chr7_3:85196094-85196113 Msa1132330:CDS 15.0%
!!! GTTTTTTTTATATGATCAAA+TGG - chr7_3:85196259-85196278 Msa1132330:CDS 15.0%
!! GAAAAAAAAACAACAAATTA+AGG + chr7_3:85196494-85196513 None:intergenic 15.0%
!!! ATTATCAGATTTTTCTTTTT+TGG + chr7_3:85197303-85197322 None:intergenic 15.0%
!!! TTTGTTTTCTTTGATGTATA+AGG - chr7_3:85195947-85195966 Msa1132330:three_prime_UTR 20.0%
!!! GTTTTTTGTATATGATCAAA+TGG - chr7_3:85196053-85196072 Msa1132330:CDS 20.0%
!! CTTTGAATTATTACAACAAT+TGG - chr7_3:85196093-85196112 Msa1132330:CDS 20.0%
!!! GTTTTTTCTATATGATCAAA+TGG - chr7_3:85196154-85196173 Msa1132330:CDS 20.0%
!! TATCACATTATTAAAACAGT+TGG - chr7_3:85196370-85196389 Msa1132330:CDS 20.0%
!! AAAATCAAATACATCATGAA+AGG + chr7_3:85197504-85197523 None:intergenic 20.0%
!!! TTTGTTTTCTTTGATGTATA+AGG - chr7_3:85195947-85195966 Msa1132330:three_prime_UTR 20.0%
!!! GTTTTTTGTATATGATCAAA+TGG - chr7_3:85196053-85196072 Msa1132330:CDS 20.0%
!! CTTTGAATTATTACAACAAT+TGG - chr7_3:85196093-85196112 Msa1132330:CDS 20.0%
!!! GTTTTTTCTATATGATCAAA+TGG - chr7_3:85196154-85196173 Msa1132330:CDS 20.0%
!! TATCACATTATTAAAACAGT+TGG - chr7_3:85196370-85196389 Msa1132330:CDS 20.0%
!! AAAATCAAATACATCATGAA+AGG + chr7_3:85197504-85197523 None:intergenic 20.0%
! ATATTCTATAATCCAGCTAT+TGG - chr7_3:85195633-85195652 Msa1132330:three_prime_UTR 25.0%
! TAATTACCAGAAAGAAAGAA+AGG + chr7_3:85195880-85195899 None:intergenic 25.0%
!! TTACAACAATTGGGTTATTT+TGG - chr7_3:85196103-85196122 Msa1132330:CDS 25.0%
!! TCTCTAAAAGAACAAATCAA+TGG + chr7_3:85196318-85196337 None:intergenic 25.0%
!!! TTTTTTCTGTCATGATCAAA+TGG - chr7_3:85196434-85196453 Msa1132330:CDS 25.0%
!! TTTTTCTGTCATGATCAAAT+GGG - chr7_3:85196435-85196454 Msa1132330:CDS 25.0%
! TCTCTATTTAGCTGATATTA+AGG - chr7_3:85196860-85196879 Msa1132330:intron 25.0%
! TTTAGCTGATATTAAGGAAT+TGG - chr7_3:85196866-85196885 Msa1132330:intron 25.0%
! ATTTGTTTAATGACCTATGT+AGG - chr7_3:85197526-85197545 Msa1132330:five_prime_UTR 25.0%
!!! ATTGAGTGTTTTAATCAACA+AGG - chr7_3:85197579-85197598 Msa1132330:five_prime_UTR 25.0%
! ATATTCTATAATCCAGCTAT+TGG - chr7_3:85195633-85195652 Msa1132330:three_prime_UTR 25.0%
! TAATTACCAGAAAGAAAGAA+AGG + chr7_3:85195880-85195899 None:intergenic 25.0%
!! TTACAACAATTGGGTTATTT+TGG - chr7_3:85196103-85196122 Msa1132330:CDS 25.0%
!! TCTCTAAAAGAACAAATCAA+TGG + chr7_3:85196318-85196337 None:intergenic 25.0%
!!! TTTTTTCTGTCATGATCAAA+TGG - chr7_3:85196434-85196453 Msa1132330:CDS 25.0%
!! TTTTTCTGTCATGATCAAAT+GGG - chr7_3:85196435-85196454 Msa1132330:CDS 25.0%
! TCTCTATTTAGCTGATATTA+AGG - chr7_3:85196860-85196879 Msa1132330:intron 25.0%
! TTTAGCTGATATTAAGGAAT+TGG - chr7_3:85196866-85196885 Msa1132330:intron 25.0%
! ATTTGTTTAATGACCTATGT+AGG - chr7_3:85197526-85197545 Msa1132330:five_prime_UTR 25.0%
!!! ATTGAGTGTTTTAATCAACA+AGG - chr7_3:85197579-85197598 Msa1132330:five_prime_UTR 25.0%
TATATTGCAATACCAATAGC+TGG + chr7_3:85195648-85195667 None:intergenic 30.0%
!! TGTGATTGATTTGAAGCTAT+TGG + chr7_3:85195697-85195716 None:intergenic 30.0%
TACTGTATCGAGTAAATCTA+GGG + chr7_3:85195722-85195741 None:intergenic 30.0%
ATACTGTATCGAGTAAATCT+AGG + chr7_3:85195723-85195742 None:intergenic 30.0%
CTTTCACATTTCATTCGATT+GGG - chr7_3:85195909-85195928 Msa1132330:three_prime_UTR 30.0%
TATCACATTATTACAGCAAC+TGG - chr7_3:85195992-85196011 Msa1132330:CDS 30.0%
ATCACATTATTACAGCAACT+GGG - chr7_3:85195993-85196012 Msa1132330:CDS 30.0%
!! TTACCATTACTAGTTGGTAA+TGG + chr7_3:85196555-85196574 None:intergenic 30.0%
TTACCAACTAGTAATGGTAA+TGG - chr7_3:85196555-85196574 Msa1132330:CDS 30.0%
ATTCCATTACCATTACTAGT+TGG + chr7_3:85196561-85196580 None:intergenic 30.0%
TTAATTGGTATGATCTCAAC+AGG + chr7_3:85196702-85196721 None:intergenic 30.0%
TGAGAAAAAGTGGTCTTTAT+TGG - chr7_3:85196733-85196752 Msa1132330:CDS 30.0%
AAGATTCTTAACACCTAGTT+TGG - chr7_3:85196797-85196816 Msa1132330:intron 30.0%
TTTGGCAAATTATCAAGGTT+GGG - chr7_3:85196815-85196834 Msa1132330:intron 30.0%
ATTGAAGACCAATTCTTTCT+AGG + chr7_3:85196990-85197009 None:intergenic 30.0%
!! TAGAAAGAATTGGTCTTCAA+TGG - chr7_3:85196989-85197008 Msa1132330:intron 30.0%
!!! TCAATGGTTTTGTATAACTG+TGG - chr7_3:85197005-85197024 Msa1132330:intron 30.0%
TCAAGGAAATTCCAAACAAA+AGG + chr7_3:85197134-85197153 None:intergenic 30.0%
! TTTGTTTGGAATTTCCTTGA+AGG - chr7_3:85197134-85197153 Msa1132330:intron 30.0%
ATTTCCTTGAAGGACATAAT+AGG - chr7_3:85197144-85197163 Msa1132330:intron 30.0%
TTTCCTTGAAGGACATAATA+GGG - chr7_3:85197145-85197164 Msa1132330:intron 30.0%
TAGAGATTTGAAGATGCATT+TGG - chr7_3:85197323-85197342 Msa1132330:intron 30.0%
! TGTTTAGAGTCAGTCAATTA+TGG - chr7_3:85197365-85197384 Msa1132330:intron 30.0%
ATGATGCAAGTTATGAGAAT+AGG - chr7_3:85197439-85197458 Msa1132330:five_prime_UTR 30.0%
TATATTGCAATACCAATAGC+TGG + chr7_3:85195648-85195667 None:intergenic 30.0%
!! TGTGATTGATTTGAAGCTAT+TGG + chr7_3:85195697-85195716 None:intergenic 30.0%
TACTGTATCGAGTAAATCTA+GGG + chr7_3:85195722-85195741 None:intergenic 30.0%
ATACTGTATCGAGTAAATCT+AGG + chr7_3:85195723-85195742 None:intergenic 30.0%
CTTTCACATTTCATTCGATT+GGG - chr7_3:85195909-85195928 Msa1132330:three_prime_UTR 30.0%
TATCACATTATTACAGCAAC+TGG - chr7_3:85195992-85196011 Msa1132330:CDS 30.0%
ATCACATTATTACAGCAACT+GGG - chr7_3:85195993-85196012 Msa1132330:CDS 30.0%
!! TTACCATTACTAGTTGGTAA+TGG + chr7_3:85196555-85196574 None:intergenic 30.0%
TTACCAACTAGTAATGGTAA+TGG - chr7_3:85196555-85196574 Msa1132330:CDS 30.0%
ATTCCATTACCATTACTAGT+TGG + chr7_3:85196561-85196580 None:intergenic 30.0%
TTAATTGGTATGATCTCAAC+AGG + chr7_3:85196702-85196721 None:intergenic 30.0%
TGAGAAAAAGTGGTCTTTAT+TGG - chr7_3:85196733-85196752 Msa1132330:CDS 30.0%
AAGATTCTTAACACCTAGTT+TGG - chr7_3:85196797-85196816 Msa1132330:intron 30.0%
TTTGGCAAATTATCAAGGTT+GGG - chr7_3:85196815-85196834 Msa1132330:intron 30.0%
ATTGAAGACCAATTCTTTCT+AGG + chr7_3:85196990-85197009 None:intergenic 30.0%
!! TAGAAAGAATTGGTCTTCAA+TGG - chr7_3:85196989-85197008 Msa1132330:intron 30.0%
!!! TCAATGGTTTTGTATAACTG+TGG - chr7_3:85197005-85197024 Msa1132330:intron 30.0%
TCAAGGAAATTCCAAACAAA+AGG + chr7_3:85197134-85197153 None:intergenic 30.0%
! TTTGTTTGGAATTTCCTTGA+AGG - chr7_3:85197134-85197153 Msa1132330:intron 30.0%
ATTTCCTTGAAGGACATAAT+AGG - chr7_3:85197144-85197163 Msa1132330:intron 30.0%
TTTCCTTGAAGGACATAATA+GGG - chr7_3:85197145-85197164 Msa1132330:intron 30.0%
TAGAGATTTGAAGATGCATT+TGG - chr7_3:85197323-85197342 Msa1132330:intron 30.0%
! TGTTTAGAGTCAGTCAATTA+TGG - chr7_3:85197365-85197384 Msa1132330:intron 30.0%
ATGATGCAAGTTATGAGAAT+AGG - chr7_3:85197439-85197458 Msa1132330:five_prime_UTR 30.0%
GAAGAGAAATAGAGAAAGAG+AGG + chr7_3:85195810-85195829 None:intergenic 35.0%
TACCAGAAAGAAAGAAAGGA+GGG + chr7_3:85195876-85195895 None:intergenic 35.0%
TTACCAGAAAGAAAGAAAGG+AGG + chr7_3:85195877-85195896 None:intergenic 35.0%
CCAATCGAATGAAATGTGAA+AGG + chr7_3:85195911-85195930 None:intergenic 35.0%
CCTTTCACATTTCATTCGAT+TGG - chr7_3:85195908-85195927 Msa1132330:three_prime_UTR 35.0%
!! TACTAGTTGGTAATGGTGAT+TGG + chr7_3:85196548-85196567 None:intergenic 35.0%
TCACCATTACCAACTAGTAA+TGG - chr7_3:85196549-85196568 Msa1132330:CDS 35.0%
AAACCCTTCAAGATCTTTGT+GGG - chr7_3:85196609-85196628 Msa1132330:CDS 35.0%
TAACCCACAAAGATCTTGAA+GGG + chr7_3:85196615-85196634 None:intergenic 35.0%
ATAACCCACAAAGATCTTGA+AGG + chr7_3:85196616-85196635 None:intergenic 35.0%
GTCGTCATTCAATCATGAAA+AGG - chr7_3:85196667-85196686 Msa1132330:CDS 35.0%
CTCAGATCTGATTGCTTAAT+TGG + chr7_3:85196717-85196736 None:intergenic 35.0%
CAATCAGATCTGAGAAAAAG+TGG - chr7_3:85196723-85196742 Msa1132330:CDS 35.0%
! CCTTGATAATTTGCCAAACT+AGG + chr7_3:85196813-85196832 None:intergenic 35.0%
CCTAGTTTGGCAAATTATCA+AGG - chr7_3:85196810-85196829 Msa1132330:intron 35.0%
GTTTGGCAAATTATCAAGGT+TGG - chr7_3:85196814-85196833 Msa1132330:intron 35.0%
GGAATTGGTAGATTTGATTG+AGG - chr7_3:85196881-85196900 Msa1132330:intron 35.0%
TCCAAAAGAGACTACAAAGA+TGG - chr7_3:85196941-85196960 Msa1132330:intron 35.0%
! TCCATCTTTGTAGTCTCTTT+TGG + chr7_3:85196945-85196964 None:intergenic 35.0%
AAAGAGACTACAAAGATGGA+TGG - chr7_3:85196945-85196964 Msa1132330:intron 35.0%
CTGTGTATCCTAGAAAGAAT+TGG - chr7_3:85196979-85196998 Msa1132330:intron 35.0%
CTTTCCTTCATAGGTTTCAA+TGG - chr7_3:85197081-85197100 Msa1132330:intron 35.0%
!!! TTGGTTCTGTACCTTTTGTT+TGG - chr7_3:85197120-85197139 Msa1132330:intron 35.0%
CTAAGGCTATCCATTATACT+CGG - chr7_3:85197195-85197214 Msa1132330:intron 35.0%
TAAGGCTATCCATTATACTC+GGG - chr7_3:85197196-85197215 Msa1132330:intron 35.0%
!! ATTGCGAATTTGCTGATCTA+TGG - chr7_3:85197246-85197265 Msa1132330:intron 35.0%
!!! TTTCTTTTTTGGCTTGAGCA+AGG + chr7_3:85197292-85197311 None:intergenic 35.0%
TATTCTTAGACCTCTTGTCA+GGG + chr7_3:85197552-85197571 None:intergenic 35.0%
TTATTCTTAGACCTCTTGTC+AGG + chr7_3:85197553-85197572 None:intergenic 35.0%
TTGCGATTATGCATTGTTCT+TGG - chr7_3:85197610-85197629 Msa1132330:five_prime_UTR 35.0%
! AGCTTCAATATAGTTTGTGC+AGG - chr7_3:85197655-85197674 Msa1132330:five_prime_UTR 35.0%
! GCTTCAATATAGTTTGTGCA+GGG - chr7_3:85197656-85197675 Msa1132330:five_prime_UTR 35.0%
! CTTCAATATAGTTTGTGCAG+GGG - chr7_3:85197657-85197676 Msa1132330:five_prime_UTR 35.0%
GAAGAGAAATAGAGAAAGAG+AGG + chr7_3:85195810-85195829 None:intergenic 35.0%
TACCAGAAAGAAAGAAAGGA+GGG + chr7_3:85195876-85195895 None:intergenic 35.0%
TTACCAGAAAGAAAGAAAGG+AGG + chr7_3:85195877-85195896 None:intergenic 35.0%
CCAATCGAATGAAATGTGAA+AGG + chr7_3:85195911-85195930 None:intergenic 35.0%
CCTTTCACATTTCATTCGAT+TGG - chr7_3:85195908-85195927 Msa1132330:three_prime_UTR 35.0%
!! TACTAGTTGGTAATGGTGAT+TGG + chr7_3:85196548-85196567 None:intergenic 35.0%
TCACCATTACCAACTAGTAA+TGG - chr7_3:85196549-85196568 Msa1132330:CDS 35.0%
AAACCCTTCAAGATCTTTGT+GGG - chr7_3:85196609-85196628 Msa1132330:CDS 35.0%
TAACCCACAAAGATCTTGAA+GGG + chr7_3:85196615-85196634 None:intergenic 35.0%
ATAACCCACAAAGATCTTGA+AGG + chr7_3:85196616-85196635 None:intergenic 35.0%
GTCGTCATTCAATCATGAAA+AGG - chr7_3:85196667-85196686 Msa1132330:CDS 35.0%
CTCAGATCTGATTGCTTAAT+TGG + chr7_3:85196717-85196736 None:intergenic 35.0%
CAATCAGATCTGAGAAAAAG+TGG - chr7_3:85196723-85196742 Msa1132330:CDS 35.0%
! CCTTGATAATTTGCCAAACT+AGG + chr7_3:85196813-85196832 None:intergenic 35.0%
CCTAGTTTGGCAAATTATCA+AGG - chr7_3:85196810-85196829 Msa1132330:intron 35.0%
GTTTGGCAAATTATCAAGGT+TGG - chr7_3:85196814-85196833 Msa1132330:intron 35.0%
GGAATTGGTAGATTTGATTG+AGG - chr7_3:85196881-85196900 Msa1132330:intron 35.0%
TCCAAAAGAGACTACAAAGA+TGG - chr7_3:85196941-85196960 Msa1132330:intron 35.0%
! TCCATCTTTGTAGTCTCTTT+TGG + chr7_3:85196945-85196964 None:intergenic 35.0%
AAAGAGACTACAAAGATGGA+TGG - chr7_3:85196945-85196964 Msa1132330:intron 35.0%
CTGTGTATCCTAGAAAGAAT+TGG - chr7_3:85196979-85196998 Msa1132330:intron 35.0%
CTTTCCTTCATAGGTTTCAA+TGG - chr7_3:85197081-85197100 Msa1132330:intron 35.0%
!!! TTGGTTCTGTACCTTTTGTT+TGG - chr7_3:85197120-85197139 Msa1132330:intron 35.0%
CTAAGGCTATCCATTATACT+CGG - chr7_3:85197195-85197214 Msa1132330:intron 35.0%
TAAGGCTATCCATTATACTC+GGG - chr7_3:85197196-85197215 Msa1132330:intron 35.0%
!! ATTGCGAATTTGCTGATCTA+TGG - chr7_3:85197246-85197265 Msa1132330:intron 35.0%
!!! TTTCTTTTTTGGCTTGAGCA+AGG + chr7_3:85197292-85197311 None:intergenic 35.0%
TATTCTTAGACCTCTTGTCA+GGG + chr7_3:85197552-85197571 None:intergenic 35.0%
TTATTCTTAGACCTCTTGTC+AGG + chr7_3:85197553-85197572 None:intergenic 35.0%
TTGCGATTATGCATTGTTCT+TGG - chr7_3:85197610-85197629 Msa1132330:five_prime_UTR 35.0%
TAGAGAAAGAGAGGATTGAG+CGG + chr7_3:85195801-85195820 None:intergenic 40.0%
! GGACTTGTTTGGAAAAAGAG+AGG + chr7_3:85195840-85195859 None:intergenic 40.0%
! CAAGTGTTGTTGGACTTGTT+TGG + chr7_3:85195851-85195870 None:intergenic 40.0%
ACCAGAAAGAAAGAAAGGAG+GGG + chr7_3:85195875-85195894 None:intergenic 40.0%
ACAGAGATGAGTTTGAGCAA+TGG - chr7_3:85196516-85196535 Msa1132330:CDS 40.0%
GAAACCCTTCAAGATCTTTG+TGG - chr7_3:85196608-85196627 Msa1132330:CDS 40.0%
TGAAATGCAATGTCTTCACG+TGG + chr7_3:85196642-85196661 None:intergenic 40.0%
ACGTGAAGACATTGCATTTC+AGG - chr7_3:85196641-85196660 Msa1132330:CDS 40.0%
GGAGTAAGAACCTTGTTCTT+AGG + chr7_3:85197035-85197054 None:intergenic 40.0%
CAAGCCATTGAAACCTATGA+AGG + chr7_3:85197088-85197107 None:intergenic 40.0%
TCATAGGTTTCAATGGCTTG+AGG - chr7_3:85197088-85197107 Msa1132330:intron 40.0%
TGGCTTGAGGATGATGAAAT+TGG - chr7_3:85197101-85197120 Msa1132330:intron 40.0%
TGATCCAACTACTTCACCTA+AGG - chr7_3:85197178-85197197 Msa1132330:intron 40.0%
! ATAGCCTTAGGTGAAGTAGT+TGG + chr7_3:85197185-85197204 None:intergenic 40.0%
!! CGAGTATAATGGATAGCCTT+AGG + chr7_3:85197197-85197216 None:intergenic 40.0%
AAGGCTATCCATTATACTCG+GGG - chr7_3:85197197-85197216 Msa1132330:intron 40.0%
TGATCTATGGCTGAATGAGA+TGG - chr7_3:85197259-85197278 Msa1132330:intron 40.0%
TAGAGAAAGAGAGGATTGAG+CGG + chr7_3:85195801-85195820 None:intergenic 40.0%
! GGACTTGTTTGGAAAAAGAG+AGG + chr7_3:85195840-85195859 None:intergenic 40.0%
! CAAGTGTTGTTGGACTTGTT+TGG + chr7_3:85195851-85195870 None:intergenic 40.0%
ACCAGAAAGAAAGAAAGGAG+GGG + chr7_3:85195875-85195894 None:intergenic 40.0%
ACAGAGATGAGTTTGAGCAA+TGG - chr7_3:85196516-85196535 Msa1132330:CDS 40.0%
GAAACCCTTCAAGATCTTTG+TGG - chr7_3:85196608-85196627 Msa1132330:CDS 40.0%
TGAAATGCAATGTCTTCACG+TGG + chr7_3:85196642-85196661 None:intergenic 40.0%
ACGTGAAGACATTGCATTTC+AGG - chr7_3:85196641-85196660 Msa1132330:CDS 40.0%
GGAGTAAGAACCTTGTTCTT+AGG + chr7_3:85197035-85197054 None:intergenic 40.0%
CAAGCCATTGAAACCTATGA+AGG + chr7_3:85197088-85197107 None:intergenic 40.0%
TCATAGGTTTCAATGGCTTG+AGG - chr7_3:85197088-85197107 Msa1132330:intron 40.0%
TGGCTTGAGGATGATGAAAT+TGG - chr7_3:85197101-85197120 Msa1132330:intron 40.0%
TGATCCAACTACTTCACCTA+AGG - chr7_3:85197178-85197197 Msa1132330:intron 40.0%
! ATAGCCTTAGGTGAAGTAGT+TGG + chr7_3:85197185-85197204 None:intergenic 40.0%
!! CGAGTATAATGGATAGCCTT+AGG + chr7_3:85197197-85197216 None:intergenic 40.0%
AAGGCTATCCATTATACTCG+GGG - chr7_3:85197197-85197216 Msa1132330:intron 40.0%
TGATCTATGGCTGAATGAGA+TGG - chr7_3:85197259-85197278 Msa1132330:intron 40.0%
TCCCCTCCTTTCTTTCTTTC+TGG - chr7_3:85195871-85195890 Msa1132330:three_prime_UTR 45.0%
GGTCTTTATTGGCGTGAAAG+AGG - chr7_3:85196744-85196763 Msa1132330:CDS 45.0%
TCAAGGTTGGGCTATGTTTG+TGG - chr7_3:85196827-85196846 Msa1132330:intron 45.0%
CTGTGGTCATCCTAAGAACA+AGG - chr7_3:85197022-85197041 Msa1132330:intron 45.0%
GTCTGTGAATTCACAGCATC+AGG + chr7_3:85197056-85197075 None:intergenic 45.0%
GATGCTGTGAATTCACAGAC+TGG - chr7_3:85197056-85197075 Msa1132330:intron 45.0%
AGACTGGTGCTTTCCTTCAT+AGG - chr7_3:85197072-85197091 Msa1132330:intron 45.0%
CAGCCCTATTATGTCCTTCA+AGG + chr7_3:85197151-85197170 None:intergenic 45.0%
CTATCCATTATACTCGGGGA+GGG - chr7_3:85197201-85197220 Msa1132330:intron 45.0%
TTCTTCCAAGCCTCAAACCA+CGG + chr7_3:85197227-85197246 None:intergenic 45.0%
TCCCCTCCTTTCTTTCTTTC+TGG - chr7_3:85195871-85195890 Msa1132330:three_prime_UTR 45.0%
GGTCTTTATTGGCGTGAAAG+AGG - chr7_3:85196744-85196763 Msa1132330:CDS 45.0%
TCAAGGTTGGGCTATGTTTG+TGG - chr7_3:85196827-85196846 Msa1132330:intron 45.0%
CTGTGGTCATCCTAAGAACA+AGG - chr7_3:85197022-85197041 Msa1132330:intron 45.0%
GTCTGTGAATTCACAGCATC+AGG + chr7_3:85197056-85197075 None:intergenic 45.0%
GATGCTGTGAATTCACAGAC+TGG - chr7_3:85197056-85197075 Msa1132330:intron 45.0%
AGACTGGTGCTTTCCTTCAT+AGG - chr7_3:85197072-85197091 Msa1132330:intron 45.0%
CAGCCCTATTATGTCCTTCA+AGG + chr7_3:85197151-85197170 None:intergenic 45.0%
CTATCCATTATACTCGGGGA+GGG - chr7_3:85197201-85197220 Msa1132330:intron 45.0%
TTCTTCCAAGCCTCAAACCA+CGG + chr7_3:85197227-85197246 None:intergenic 45.0%
TATAACCCTCCCTCCACTGT+CGG - chr7_3:85195760-85195779 Msa1132330:three_prime_UTR 50.0%
ACATGTTCACCGACAGTGGA+GGG + chr7_3:85195772-85195791 None:intergenic 50.0%
! AAGGAGGGGACAAGTGTTGT+TGG + chr7_3:85195861-85195880 None:intergenic 50.0%
GCTATCCATTATACTCGGGG+AGG - chr7_3:85197200-85197219 Msa1132330:intron 50.0%
TATAACCCTCCCTCCACTGT+CGG - chr7_3:85195760-85195779 Msa1132330:three_prime_UTR 50.0%
ACATGTTCACCGACAGTGGA+GGG + chr7_3:85195772-85195791 None:intergenic 50.0%
! AAGGAGGGGACAAGTGTTGT+TGG + chr7_3:85195861-85195880 None:intergenic 50.0%
GCTATCCATTATACTCGGGG+AGG - chr7_3:85197200-85197219 Msa1132330:intron 50.0%
CACATGTTCACCGACAGTGG+AGG + chr7_3:85195773-85195792 None:intergenic 55.0%
ACGCACATGTTCACCGACAG+TGG + chr7_3:85195776-85195795 None:intergenic 55.0%
CCTCTTGTCAGGGCCTACAT+AGG + chr7_3:85197542-85197561 None:intergenic 55.0%
CCTATGTAGGCCCTGACAAG+AGG - chr7_3:85197539-85197558 Msa1132330:five_prime_UTR 55.0%
CACATGTTCACCGACAGTGG+AGG + chr7_3:85195773-85195792 None:intergenic 55.0%
ACGCACATGTTCACCGACAG+TGG + chr7_3:85195776-85195795 None:intergenic 55.0%
CCTCTTGTCAGGGCCTACAT+AGG + chr7_3:85197542-85197561 None:intergenic 55.0%
CCTATGTAGGCCCTGACAAG+AGG - chr7_3:85197539-85197558 Msa1132330:five_prime_UTR 55.0%
GTTCACCGACAGTGGAGGGA+GGG + chr7_3:85195768-85195787 None:intergenic 60.0%
TGTTCACCGACAGTGGAGGG+AGG + chr7_3:85195769-85195788 None:intergenic 60.0%
ACGGCCCTCCCCGAGTATAA+TGG + chr7_3:85197208-85197227 None:intergenic 60.0%
ATTATACTCGGGGAGGGCCG+TGG - chr7_3:85197207-85197226 Msa1132330:intron 60.0%
GTTCACCGACAGTGGAGGGA+GGG + chr7_3:85195768-85195787 None:intergenic 60.0%
TGTTCACCGACAGTGGAGGG+AGG + chr7_3:85195769-85195788 None:intergenic 60.0%
ACGGCCCTCCCCGAGTATAA+TGG + chr7_3:85197208-85197227 None:intergenic 60.0%
ATTATACTCGGGGAGGGCCG+TGG - chr7_3:85197207-85197226 Msa1132330:intron 60.0%
! GAGGGCCGTGGTTTGAGGCT+TGG - chr7_3:85197219-85197238 Msa1132330:intron 65.0%
! GAGGGCCGTGGTTTGAGGCT+TGG - chr7_3:85197219-85197238 Msa1132330:intron 65.0%
TCGGGGAGGGCCGTGGTTTG+AGG - chr7_3:85197214-85197233 Msa1132330:intron 70.0%
TCGGGGAGGGCCGTGGTTTG+AGG - chr7_3:85197214-85197233 Msa1132330:intron 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 85195621 85197677 85195621 ID=Msa1132330;Name=Msa1132330
chr7_3 mRNA 85195621 85197677 85195621 ID=Msa1132330-mRNA-1;Parent=Msa1132330;Name=Msa1132330-mRNA-1;_AED=0.00;_eAED=0.00;_QI=272|0|0.5|1|0|0.5|2|352|267
chr7_3 exon 85195621 85196776 85195621 ID=Msa1132330-mRNA-1:exon:25457;Parent=Msa1132330-mRNA-1
chr7_3 exon 85197406 85197677 85197406 ID=Msa1132330-mRNA-1:exon:25456;Parent=Msa1132330-mRNA-1
chr7_3 five_prime_UTR 85197406 85197677 85197406 ID=Msa1132330-mRNA-1:five_prime_utr;Parent=Msa1132330-mRNA-1
chr7_3 CDS 85195973 85196776 85195973 ID=Msa1132330-mRNA-1:cds;Parent=Msa1132330-mRNA-1
chr7_3 three_prime_UTR 85195621 85195972 85195621 ID=Msa1132330-mRNA-1:three_prime_utr;Parent=Msa1132330-mRNA-1
Gene Sequence

>Msa1132330

ATGAGTTTGAGCAATGGTAAAAACCAATCACCATTACCAACTAGTAATGGTAATGGAATAGATTCTGCTGAGAATGAGAATGCAAAGAAACCCTTCAAGATCTTTGTGGGTTATGATCCACGTGAAGACATTGCATTTCAGGTATGTCGTCATTCAATCATGAAAAGGTCTTCAATTCCTGTTGAGATCATACCAATTAAGCAATCAGATCTGAGAAAAAGTGGTCTTTATTGGCGTGAAAGAGGTCAATTTGAGAGCACTGAGTTTTCTTTTACAAGATTCTTAACACCTAGTTTGGCAAATTATCAAGGTTGGGCTATGTTTGTGGATTGTGATTTTCTCTATTTAGCTGATATTAAGGAATTGGTAGATTTGATTGAGGATAAGTATGCTATTATGTGTGTTCAACATGATTATACTCCAAAAGAGACTACAAAGATGGATGGTGCTGTTCAAACTGTGTATCCTAGAAAGAATTGGTCTTCAATGGTTTTGTATAACTGTGGTCATCCTAAGAACAAGGTTCTTACTCCTGATGCTGTGAATTCACAGACTGGTGCTTTCCTTCATAGGTTTCAATGGCTTGAGGATGATGAAATTGGTTCTGTACCTTTTGTTTGGAATTTCCTTGAAGGACATAATAGGGCTGTTGAGAATGATCCAACTACTTCACCTAAGGCTATCCATTATACTCGGGGAGGGCCGTGGTTTGAGGCTTGGAAGAATTGCGAATTTGCTGATCTATGGCTGAATGAGATGGAAGAGTACCTTGCTCAAGCCAAAAAAGAAAAATCTGATAATTAG
Protein sequence

>Msa1132330

MSLSNGKNQSPLPTSNGNGIDSAENENAKKPFKIFVGYDPREDIAFQVCRHSIMKRSSIPVEIIPIKQSDLRKSGLYWRERGQFESTEFSFTRFLTPSLANYQGWAMFVDCDFLYLADIKELVDLIEDKYAIMCVQHDYTPKETTKMDGAVQTVYPRKNWSSMVLYNCGHPKNKVLTPDAVNSQTGAFLHRFQWLEDDEIGSVPFVWNFLEGHNRAVENDPTTSPKAIHYTRGGPWFEAWKNCEFADLWLNEMEEYLAQAKKEKSDN*