AlfalfaGEDB | Msa0756030

M. sativa cultivar zhongmu-4 / Msa0756030

KEGG: K14682
GO: -
PFAM: PF00696.31

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0756030	sp\|B5X4Z4\|NAGS2_ARATH	66.389	601	180	8	15	601	18	610	0.0	797

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0756030	G7K2G8	97.377	610	14	2	1	609	1	609	0.0	1215

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TFs/TRs:

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Gene ID	Type	Classification
Msa0756030	TR	GNAT

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0046100	Msa0756030	0.802663	5.041121e-49	-8.615850e-47
Msa0270270	Msa0756030	0.800944	1.136269e-48	-8.615850e-47
Msa0275600	Msa0756030	0.800401	1.466254e-48	-8.615850e-47
Msa0298390	Msa0756030	-0.809020	2.326054e-50	-8.615850e-47
Msa0378340	Msa0756030	0.814322	1.634291e-51	-8.615850e-47
Msa0427690	Msa0756030	0.810837	9.449858e-51	-8.615850e-47
Msa0518520	Msa0756030	0.807811	4.212669e-50	-8.615850e-47
Msa0627910	Msa0756030	0.806742	7.097351e-50	-8.615850e-47
Msa0713790	Msa0756030	0.933232	2.565942e-95	-8.615850e-47
Msa0756030	Msa0775260	0.811002	8.703663e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0756030	MtrunA17_Chr5g0401051	95.574	610	14	3	1	609	1	598	0.0	1186

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0756030	AT4G37670.2	66.389	601	180	8	15	601	18	610	797
Msa0756030	AT2G22910.1	70.209	527	154	2	78	601	75	601	768
Msa0756030	AT4G37670.1	65.226	532	163	8	15	532	18	541	681

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Find 158 sgRNAs with CRISPR-Local

Find 311 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACATGTCTGCTTCTTCAAG+AGG	0.505053	5_2:-10041056	None:intergenic
AACGAGGAATTAAAGGAGCG+AGG	0.605298	5_2:-10039033	None:intergenic
AACGCTGCAATGTGTGCAGC+AGG	0.498191	5_2:+10040245	Msa0756030:CDS
AACGTCTAAGTCGAATGAAT+GGG	0.522685	5_2:+10041309	Msa0756030:CDS
AACTTCTTGACAGGTTTGTG+AGG	0.570293	5_2:+10043423	Msa0756030:intron
AACTTGGGCTACTCCAGCTC+TGG	0.523097	5_2:+10040632	Msa0756030:CDS
AAGAGATGGGATGGGAACAA+TGG	0.612647	5_2:+10041740	Msa0756030:CDS
AAGAGTGGAATTACTGTTGG+TGG	0.488225	5_2:+10043551	Msa0756030:CDS
AAGCAACAAATTTGTCCAAG+CGG	0.643686	5_2:+10038830	Msa0756030:five_prime_UTR
AAGCTTCTACCAAATAAGAG+TGG	0.625840	5_2:+10043536	Msa0756030:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTTAGTAACAGTAATGTTTC+TGG	+	chr5_2:10039524-10039543	Msa0756030:intron	30.0%
AATGTTGTCAATTTGCACAT+GGG	-	chr5_2:10039608-10039627	None:intergenic	30.0%
GACAACATTCTCAAAGATAA+AGG	+	chr5_2:10039619-10039638	Msa0756030:CDS	30.0%
TCTCAAAGATAAAGGTTAGT+TGG	+	chr5_2:10039627-10039646	Msa0756030:intron	30.0%
TGCATCTAGTAACTTGAAAA+TGG	+	chr5_2:10039931-10039950	Msa0756030:intron	30.0%
ACTTGAAAATGGCTTATAGT+TGG	+	chr5_2:10039942-10039961	Msa0756030:intron	30.0%
CTTGAAAATGGCTTATAGTT+GGG	+	chr5_2:10039943-10039962	Msa0756030:intron	30.0%
CAGTAACATGGTCAATTATA+AGG	-	chr5_2:10040163-10040182	None:intergenic	30.0%
ATGTCTACGAATATTGCATA+TGG	-	chr5_2:10040315-10040334	None:intergenic	30.0%
ATATGCAATATTCGTAGACA+TGG	+	chr5_2:10040314-10040333	Msa0756030:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	10038814	10043811	10038814	ID=Msa0756030;Name=Msa0756030
chr5_2	mRNA	10038814	10043811	10038814	ID=Msa0756030-mRNA-1;Parent=Msa0756030;Name=Msa0756030-mRNA-1;_AED=0.16;_eAED=0.16;_QI=102\|1\|1\|1\|1\|1\|10\|216\|609
chr5_2	exon	10038814	10039269	10038814	ID=Msa0756030-mRNA-1:exon:4613;Parent=Msa0756030-mRNA-1
chr5_2	exon	10039553	10039640	10039553	ID=Msa0756030-mRNA-1:exon:4614;Parent=Msa0756030-mRNA-1
chr5_2	exon	10040192	10040400	10040192	ID=Msa0756030-mRNA-1:exon:4615;Parent=Msa0756030-mRNA-1
chr5_2	exon	10040521	10040672	10040521	ID=Msa0756030-mRNA-1:exon:4616;Parent=Msa0756030-mRNA-1
chr5_2	exon	10040947	10041370	10040947	ID=Msa0756030-mRNA-1:exon:4617;Parent=Msa0756030-mRNA-1
chr5_2	exon	10041672	10041769	10041672	ID=Msa0756030-mRNA-1:exon:4618;Parent=Msa0756030-mRNA-1
chr5_2	exon	10042299	10042410	10042299	ID=Msa0756030-mRNA-1:exon:4619;Parent=Msa0756030-mRNA-1
chr5_2	exon	10042522	10042681	10042522	ID=Msa0756030-mRNA-1:exon:4620;Parent=Msa0756030-mRNA-1
chr5_2	exon	10043189	10043261	10043189	ID=Msa0756030-mRNA-1:exon:4621;Parent=Msa0756030-mRNA-1
chr5_2	exon	10043436	10043811	10043436	ID=Msa0756030-mRNA-1:exon:4622;Parent=Msa0756030-mRNA-1
chr5_2	five_prime_UTR	10038814	10038915	10038814	ID=Msa0756030-mRNA-1:five_prime_utr;Parent=Msa0756030-mRNA-1
chr5_2	CDS	10038916	10039269	10038916	ID=Msa0756030-mRNA-1:cds;Parent=Msa0756030-mRNA-1
chr5_2	CDS	10039553	10039640	10039553	ID=Msa0756030-mRNA-1:cds;Parent=Msa0756030-mRNA-1
chr5_2	CDS	10040192	10040400	10040192	ID=Msa0756030-mRNA-1:cds;Parent=Msa0756030-mRNA-1
chr5_2	CDS	10040521	10040672	10040521	ID=Msa0756030-mRNA-1:cds;Parent=Msa0756030-mRNA-1
chr5_2	CDS	10040947	10041370	10040947	ID=Msa0756030-mRNA-1:cds;Parent=Msa0756030-mRNA-1
chr5_2	CDS	10041672	10041769	10041672	ID=Msa0756030-mRNA-1:cds;Parent=Msa0756030-mRNA-1
chr5_2	CDS	10042299	10042410	10042299	ID=Msa0756030-mRNA-1:cds;Parent=Msa0756030-mRNA-1
chr5_2	CDS	10042522	10042681	10042522	ID=Msa0756030-mRNA-1:cds;Parent=Msa0756030-mRNA-1
chr5_2	CDS	10043189	10043261	10043189	ID=Msa0756030-mRNA-1:cds;Parent=Msa0756030-mRNA-1
chr5_2	CDS	10043436	10043595	10043436	ID=Msa0756030-mRNA-1:cds;Parent=Msa0756030-mRNA-1
chr5_2	three_prime_UTR	10043596	10043811	10043596	ID=Msa0756030-mRNA-1:three_prime_utr;Parent=Msa0756030-mRNA-1

Gene Sequence

>Msa0756030

ATGGCTCCTTCTTTATCGCGCCTCTATACTTCCCTTCCAAACCCTATCAACTTCACCACTTCATCTTCTCGCCAATACTCTCACTTCAATCCTCAACAACTTAATCTCCAATTCAAACCTCGCTCCTTTAATTCCTCGTTTTTCACCGCTCAAAATCACGAAACAATGACAAAACGCAATGCGTTGAACAACGAAGGAGTGAACGGAATCACTGAAGAAGGAGATGAACAACAACAATTCATTCGTTCCCTTCGTGAAGCTTGGCCTTATCTTCTCGCTTATCGTAGAACCACTTTTGTTCTCGTCATTTCTGCTGAAATTGTTGATAGTCCTTCTTTGGATCCCATTCTCAAGGATATAGCTTTTCTTCATCACCTTGGAATCCACTTTGTTATTGTTCCTGGCACCCATGTGCAAATTGACAACATTCTCAAAGATAAAGGGAGCAAGGCTAAGTATGTTGGGAGATATAGAATAACAGACGATGAATCTCTAAACGCTGCAATGTGTGCAGCAGGAGGAATAATGTCGATGATAGCAGCTAAACTTTCAGTTGGACCTTCCATATGCAATATTCGTAGACATGGTGATAATAGTCGATTGCATGACGTTAGTGTCAGTGTTACAAGTGGTAACTTTCTTGCAGCCAAGAGAAGAGGGGTGGTCGGTGGAATTGATTATGGGTCAACTGGGGAAGTTAAAAAAATAGACGCTACTCGCATGCGTGAGAGACTTGATGGTGGTTCTATAGTTATTTTAACCAACTTGGGCTACTCCAGCTCTGGTGAAGTATTGAATTGCAACACCTATGAGGTTGCAACAGCTTGTGCCTTGGCAATTGGGGCGGACAAGTTAATATGCGTTATAGATGGTCCAATACTTGATGAAAATGGACGCCTGATTCGTTTCTTGCCTCTTGAAGAAGCAGACATGTTAATTCGCAAGCGGGCCGAGCAAAGTGAAATAGCAGCTAACTATGTGAAAGCGGTTGAGGAAGAAGGTTTAAATCCTGTTGAGCATAACAATTTTAACGGGGCAGTCAAATCTCCACCTAATGGAAAACGCATTACAGAATGGCATAATGCAACTTTCCACAATGGTGTTGGTTTTGAGAATGGAGATGGCTTAGGGACTAGCGAGCAAGGCTTTGCTATTGGAGGTCAAGAACGTCTAAGTCGAATGAATGGGTACCTGTCAGAATTGGCTGCTGCAGCTTTTGTTTGCAGGGGAGGGGTTCAAAGAGTTCACTTGTTGGATGGCACTATTAGTGGAGTTTTATTGTTGGAATTGTTTACAAGAGATGGGATGGGAACAATGGTGGCTAGTGACCTTTACGAAGGCACTCGAAAAGCAGAGAAAGAAGATTTTTCTGGAATCAAACAACTTATTCAGCCTTTGGAGGCATGTGGCATACTGGTTAAGCGGTCTGATGAAGAGCTGCTACAAACGTTGGATAATTTCGTTGTTGTGGAAAGAGAAGGTCAAATAATTGCATGTGGTGGCCTTTTTCCTTACCCTGAGGAGAAGTGTGCAGAGGTTGCTGCTATTGCAGTATCTCCTGATTGCCGTGGCCAAGGGCAGGGAGACAAATTGCTTGATTATATGGAGAAGAAAGCAGCATCTCTTGGATTCAATAAGCTTTTCCTACTTACAACACGTACAGCAGATTGGTTTGTGAGGCGTGGATTTTCAGAATGTTCAATTGATAATATTCCTGAAAAGAAAAAGAAAACAATCAACATGTCCCGCAATTCTAAGTATTATATGAAGAAGCTTCTACCAAATAAGAGTGGAATTACTGTTGGTGGAAACAAATTAGTTACAAATTAG

Protein sequence

>Msa0756030

MAPSLSRLYTSLPNPINFTTSSSRQYSHFNPQQLNLQFKPRSFNSSFFTAQNHETMTKRNALNNEGVNGITEEGDEQQQFIRSLREAWPYLLAYRRTTFVLVISAEIVDSPSLDPILKDIAFLHHLGIHFVIVPGTHVQIDNILKDKGSKAKYVGRYRITDDESLNAAMCAAGGIMSMIAAKLSVGPSICNIRRHGDNSRLHDVSVSVTSGNFLAAKRRGVVGGIDYGSTGEVKKIDATRMRERLDGGSIVILTNLGYSSSGEVLNCNTYEVATACALAIGADKLICVIDGPILDENGRLIRFLPLEEADMLIRKRAEQSEIAANYVKAVEEEGLNPVEHNNFNGAVKSPPNGKRITEWHNATFHNGVGFENGDGLGTSEQGFAIGGQERLSRMNGYLSELAAAAFVCRGGVQRVHLLDGTISGVLLLELFTRDGMGTMVASDLYEGTRKAEKEDFSGIKQLIQPLEACGILVKRSDEELLQTLDNFVVVEREGQIIACGGLFPYPEEKCAEVAAIAVSPDCRGQGQGDKLLDYMEKKAASLGFNKLFLLTTRTADWFVRRGFSECSIDNIPEKKKKTINMSRNSKYYMKKLLPNKSGITVGGNKLVTN*