Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0837660 | RHN53916.1 | 91.128 | 541 | 5 | 2 | 3 | 501 | 59 | 598 | 0.0 | 992 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0837660 | sp|B5X4Z4|NAGS2_ARATH | 64.739 | 536 | 131 | 4 | 16 | 493 | 75 | 610 | 0.0 | 688 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0837660 | A0A396HKT9 | 91.128 | 541 | 5 | 2 | 3 | 501 | 59 | 598 | 0.0 | 992 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0837660 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004860 | Msa0837660 | 0.802475 | 5.513438e-49 | -8.615850e-47 |
| Msa0004920 | Msa0837660 | 0.800138 | 1.658648e-48 | -8.615850e-47 |
| Msa0004980 | Msa0837660 | 0.803443 | 3.477400e-49 | -8.615850e-47 |
| Msa0008340 | Msa0837660 | 0.800593 | 1.340333e-48 | -8.615850e-47 |
| Msa0008410 | Msa0837660 | 0.805637 | 1.212646e-49 | -8.615850e-47 |
| Msa0013050 | Msa0837660 | -0.806144 | 9.485115e-50 | -8.615850e-47 |
| Msa0021810 | Msa0837660 | 0.824651 | 7.190124e-54 | -8.615850e-47 |
| Msa0032840 | Msa0837660 | -0.841986 | 3.406174e-58 | -8.615850e-47 |
| Msa0047980 | Msa0837660 | 0.819403 | 1.183125e-52 | -8.615850e-47 |
| Msa0049650 | Msa0837660 | -0.810270 | 1.252772e-50 | -8.615850e-47 |
| Msa0064350 | Msa0837660 | 0.865287 | 6.587042e-65 | -8.615850e-47 |
| Msa0064380 | Msa0837660 | 0.827441 | 1.562248e-54 | -8.615850e-47 |
| Msa0073670 | Msa0837660 | 0.804946 | 1.692505e-49 | -8.615850e-47 |
| Msa0084650 | Msa0837660 | 0.842503 | 2.485711e-58 | -8.615850e-47 |
| Msa0114540 | Msa0837660 | -0.814030 | 1.896018e-51 | -8.615850e-47 |
| Msa0115390 | Msa0837660 | 0.801541 | 8.576497e-49 | -8.615850e-47 |
| Msa0607340 | Msa0837660 | -0.809757 | 1.616348e-50 | -8.615850e-47 |
| Msa0622960 | Msa0837660 | 0.808675 | 2.756113e-50 | -8.615850e-47 |
| Msa0623700 | Msa0837660 | 0.813034 | 3.140118e-51 | -8.615850e-47 |
| Msa0625530 | Msa0837660 | -0.803571 | 3.271041e-49 | -8.615850e-47 |
| Msa0627910 | Msa0837660 | 0.847211 | 1.335235e-59 | -8.615850e-47 |
| Msa0647490 | Msa0837660 | 0.818549 | 1.850014e-52 | -8.615850e-47 |
| Msa0663900 | Msa0837660 | 0.813299 | 2.745356e-51 | -8.615850e-47 |
| Msa0673010 | Msa0837660 | -0.831978 | 1.228222e-55 | -8.615850e-47 |
| Msa0680240 | Msa0837660 | 0.819331 | 1.228548e-52 | -8.615850e-47 |
| Msa0682780 | Msa0837660 | 0.813259 | 2.801706e-51 | -8.615850e-47 |
| Msa0687630 | Msa0837660 | 0.835311 | 1.806788e-56 | -8.615850e-47 |
| Msa0690160 | Msa0837660 | 0.825666 | 4.139276e-54 | -8.615850e-47 |
| Msa0692230 | Msa0837660 | 0.809641 | 1.711683e-50 | -8.615850e-47 |
| Msa0706960 | Msa0837660 | 0.812327 | 4.482776e-51 | -8.615850e-47 |
| Msa0713790 | Msa0837660 | 0.962991 | 1.532489e-121 | -8.615850e-47 |
| Msa0713820 | Msa0837660 | 0.841703 | 4.046703e-58 | -8.615850e-47 |
| Msa0743260 | Msa0837660 | 0.815713 | 8.027304e-52 | -8.615850e-47 |
| Msa0756030 | Msa0837660 | 0.914305 | 2.239590e-84 | -8.615850e-47 |
| Msa0756060 | Msa0837660 | 0.815511 | 8.905287e-52 | -8.615850e-47 |
| Msa0775260 | Msa0837660 | 0.808770 | 2.630086e-50 | -8.615850e-47 |
| Msa0778320 | Msa0837660 | 0.805881 | 1.077375e-49 | -8.615850e-47 |
| Msa0787750 | Msa0837660 | 0.829964 | 3.834080e-55 | -8.615850e-47 |
| Msa0797750 | Msa0837660 | 0.870102 | 1.880013e-66 | -8.615850e-47 |
| Msa0806920 | Msa0837660 | 0.816017 | 6.867215e-52 | -8.615850e-47 |
| Msa0824640 | Msa0837660 | 0.817548 | 3.115043e-52 | -8.615850e-47 |
| Msa0833310 | Msa0837660 | 0.809288 | 2.037283e-50 | -8.615850e-47 |
| Msa0835280 | Msa0837660 | 0.851547 | 8.268933e-61 | -8.615850e-47 |
| Msa0837660 | Msa0864010 | 0.818537 | 1.861069e-52 | -8.615850e-47 |
| Msa0837660 | Msa0900630 | 0.819158 | 1.345231e-52 | -8.615850e-47 |
| Msa0837660 | Msa0937670 | 0.812834 | 3.472745e-51 | -8.615850e-47 |
| Msa0837660 | Msa1046020 | 0.810345 | 1.207373e-50 | -8.615850e-47 |
| Msa0837660 | Msa1046060 | 0.805563 | 1.256977e-49 | -8.615850e-47 |
| Msa0837660 | Msa1072460 | -0.803123 | 4.050949e-49 | -8.615850e-47 |
| Msa0837660 | Msa1082700 | 0.831106 | 2.014934e-55 | -8.615850e-47 |
| Msa0837660 | Msa1109570 | 0.841630 | 4.230270e-58 | -8.615850e-47 |
| Msa0837660 | Msa1127830 | 0.801336 | 9.448642e-49 | -8.615850e-47 |
| Msa0837660 | Msa1134110 | 0.815721 | 7.994706e-52 | -8.615850e-47 |
| Msa0837660 | Msa1143840 | 0.800734 | 1.254354e-48 | -8.615850e-47 |
| Msa0837660 | Msa1165870 | 0.808481 | 3.032721e-50 | -8.615850e-47 |
| Msa0837660 | Msa1171870 | 0.819498 | 1.125362e-52 | -8.615850e-47 |
| Msa0837660 | Msa1184370 | 0.808668 | 2.766002e-50 | -8.615850e-47 |
| Msa0837660 | Msa1199990 | 0.820698 | 5.978311e-53 | -8.615850e-47 |
| Msa0837660 | Msa1218960 | 0.829356 | 5.387998e-55 | -8.615850e-47 |
| Msa0837660 | Msa1225150 | -0.801042 | 1.084940e-48 | -8.615850e-47 |
| Msa0837660 | Msa1260130 | 0.810949 | 8.936328e-51 | -8.615850e-47 |
| Msa0837660 | Msa1267260 | 0.808093 | 3.668900e-50 | -8.615850e-47 |
| Msa0837660 | Msa1269300 | 0.804156 | 2.473318e-49 | -8.615850e-47 |
| Msa0837660 | Msa1288170 | 0.810341 | 1.209867e-50 | -8.615850e-47 |
| Msa0837660 | Msa1303780 | 0.809054 | 2.287095e-50 | -8.615850e-47 |
| Msa0837660 | Msa1304820 | 0.869423 | 3.130614e-66 | -8.615850e-47 |
| Msa0837660 | Msa1306430 | 0.824093 | 9.728797e-54 | -8.615850e-47 |
| Msa0837660 | Msa1307950 | 0.812890 | 3.376101e-51 | -8.615850e-47 |
| Msa0837660 | Msa1310690 | 0.810920 | 9.066334e-51 | -8.615850e-47 |
| Msa0837660 | Msa1335940 | 0.813520 | 2.455677e-51 | -8.615850e-47 |
| Msa0837660 | Msa1336950 | 0.825253 | 5.183390e-54 | -8.615850e-47 |
| Msa0837660 | Msa1340020 | 0.814377 | 1.588844e-51 | -8.615850e-47 |
| Msa0837660 | Msa1340650 | 0.804035 | 2.620789e-49 | -8.615850e-47 |
| Msa0837660 | Msa1345360 | 0.826382 | 2.798159e-54 | -8.615850e-47 |
| Msa0837660 | Msa1363120 | 0.842385 | 2.670439e-58 | -8.615850e-47 |
| Msa0837660 | Msa1364910 | 0.861319 | 1.115590e-63 | -8.615850e-47 |
| Msa0837660 | Msa1365150 | 0.811637 | 6.336353e-51 | -8.615850e-47 |
| Msa0837660 | Msa1367880 | 0.805776 | 1.133914e-49 | -8.615850e-47 |
| Msa0837660 | Msa1370340 | 0.835454 | 1.661988e-56 | -8.615850e-47 |
| Msa0837660 | Msa1370860 | 0.803751 | 3.001328e-49 | -8.615850e-47 |
| Msa0837660 | Msa1373330 | 0.819194 | 1.320260e-52 | -8.615850e-47 |
| Msa0837660 | Msa1382720 | 0.804840 | 1.781074e-49 | -8.615850e-47 |
| Msa0837660 | Msa1392200 | 0.816059 | 6.722022e-52 | -8.615850e-47 |
| Msa0837660 | Msa1393740 | 0.818419 | 1.979610e-52 | -8.615850e-47 |
| Msa0837660 | Msa1394500 | 0.806593 | 7.628921e-50 | -8.615850e-47 |
| Msa0837660 | Msa1397810 | 0.812336 | 4.462950e-51 | -8.615850e-47 |
| Msa0837660 | Msa1404010 | 0.815198 | 1.045113e-51 | -8.615850e-47 |
| Msa0837660 | Msa1404190 | 0.829206 | 5.859363e-55 | -8.615850e-47 |
| Msa0837660 | Msa1408790 | 0.805729 | 1.159928e-49 | -8.615850e-47 |
| Msa0837660 | Msa1423910 | 0.812444 | 4.226329e-51 | -8.615850e-47 |
| Msa0837660 | Msa1435750 | 0.853987 | 1.660764e-61 | -8.615850e-47 |
| Msa0837660 | Msa1453780 | -0.810313 | 1.226408e-50 | -8.615850e-47 |
| Msa0837660 | Msa1460470 | 0.800037 | 1.739168e-48 | -8.615850e-47 |
| Msa0837660 | Msa1465080 | 0.813658 | 2.289338e-51 | -8.615850e-47 |
| Msa0837660 | Msa1465960 | 0.802029 | 6.809500e-49 | -8.615850e-47 |
| Msa0143080 | Msa0837660 | 0.807934 | 3.966341e-50 | -8.615850e-47 |
| Msa0143430 | Msa0837660 | 0.807350 | 5.277332e-50 | -8.615850e-47 |
| Msa0148100 | Msa0837660 | 0.805694 | 1.179392e-49 | -8.615850e-47 |
| Msa0155430 | Msa0837660 | 0.800419 | 1.454179e-48 | -8.615850e-47 |
| Msa0172960 | Msa0837660 | -0.822406 | 2.409057e-53 | -8.615850e-47 |
| Msa0183460 | Msa0837660 | 0.802454 | 5.567193e-49 | -8.615850e-47 |
| Msa0188630 | Msa0837660 | 0.817116 | 3.897202e-52 | -8.615850e-47 |
| Msa0191050 | Msa0837660 | 0.834398 | 3.067717e-56 | -8.615850e-47 |
| Msa0194480 | Msa0837660 | 0.844811 | 5.999130e-59 | -8.615850e-47 |
| Msa0222920 | Msa0837660 | 0.827009 | 1.982484e-54 | -8.615850e-47 |
| Msa0230550 | Msa0837660 | -0.832094 | 1.149856e-55 | -8.615850e-47 |
| Msa0233350 | Msa0837660 | 0.833293 | 5.795072e-56 | -8.615850e-47 |
| Msa0236270 | Msa0837660 | 0.830334 | 3.114608e-55 | -8.615850e-47 |
| Msa0236980 | Msa0837660 | 0.818347 | 2.055541e-52 | -8.615850e-47 |
| Msa0369050 | Msa0837660 | 0.801143 | 1.034674e-48 | -8.615850e-47 |
| Msa0376690 | Msa0837660 | 0.803003 | 4.288439e-49 | -8.615850e-47 |
| Msa0380040 | Msa0837660 | 0.836250 | 1.044395e-56 | -8.615850e-47 |
| Msa0394400 | Msa0837660 | 0.813182 | 2.914062e-51 | -8.615850e-47 |
| Msa0402330 | Msa0837660 | 0.827831 | 1.258915e-54 | -8.615850e-47 |
| Msa0414950 | Msa0837660 | -0.818452 | 1.946074e-52 | -8.615850e-47 |
| Msa0415430 | Msa0837660 | 0.800122 | 1.671513e-48 | -8.615850e-47 |
| Msa0429290 | Msa0837660 | 0.828619 | 8.127412e-55 | -8.615850e-47 |
| Msa0441130 | Msa0837660 | 0.812723 | 3.673629e-51 | -8.615850e-47 |
| Msa0449820 | Msa0837660 | 0.812498 | 4.112711e-51 | -8.615850e-47 |
| Msa0449830 | Msa0837660 | 0.824516 | 7.738700e-54 | -8.615850e-47 |
| Msa0471590 | Msa0837660 | 0.803668 | 3.122965e-49 | -8.615850e-47 |
| Msa0475580 | Msa0837660 | 0.825634 | 4.211041e-54 | -8.615850e-47 |
| Msa0481480 | Msa0837660 | 0.816282 | 5.993515e-52 | -8.615850e-47 |
| Msa0241780 | Msa0837660 | 0.802575 | 5.256685e-49 | -8.615850e-47 |
| Msa0258920 | Msa0837660 | -0.812097 | 5.033116e-51 | -8.615850e-47 |
| Msa0264350 | Msa0837660 | 0.816576 | 5.148917e-52 | -8.615850e-47 |
| Msa0275600 | Msa0837660 | 0.802142 | 6.455710e-49 | -8.615850e-47 |
| Msa0282250 | Msa0837660 | 0.803348 | 3.638232e-49 | -8.615850e-47 |
| Msa0294390 | Msa0837660 | 0.800464 | 1.423746e-48 | -8.615850e-47 |
| Msa0296760 | Msa0837660 | 0.801733 | 7.832418e-49 | -8.615850e-47 |
| Msa0298390 | Msa0837660 | -0.802605 | 5.181771e-49 | -8.615850e-47 |
| Msa0310410 | Msa0837660 | 0.803238 | 3.835660e-49 | -8.615850e-47 |
| Msa0311700 | Msa0837660 | 0.803891 | 2.807226e-49 | -8.615850e-47 |
| Msa0312990 | Msa0837660 | 0.837952 | 3.836278e-57 | -8.615850e-47 |
| Msa0328210 | Msa0837660 | 0.821273 | 4.408387e-53 | -8.615850e-47 |
| Msa0343810 | Msa0837660 | 0.831211 | 1.897926e-55 | -8.615850e-47 |
| Msa0347680 | Msa0837660 | 0.800098 | 1.690106e-48 | -8.615850e-47 |
| Msa0355270 | Msa0837660 | 0.825147 | 5.492350e-54 | -8.615850e-47 |
| Msa0485850 | Msa0837660 | 0.820337 | 7.234029e-53 | -8.615850e-47 |
| Msa0508590 | Msa0837660 | 0.811137 | 8.137622e-51 | -8.615850e-47 |
| Msa0508630 | Msa0837660 | 0.809927 | 1.485785e-50 | -8.615850e-47 |
| Msa0519220 | Msa0837660 | 0.819588 | 1.073500e-52 | -8.615850e-47 |
| Msa0552810 | Msa0837660 | 0.824019 | 1.012595e-53 | -8.615850e-47 |
| Msa0553220 | Msa0837660 | -0.800875 | 1.173898e-48 | -8.615850e-47 |
| Msa0591770 | Msa0837660 | 0.801110 | 1.050812e-48 | -8.615850e-47 |
| Msa0594910 | Msa0837660 | 0.800754 | 1.242324e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0837660 | MtrunA17_Chr5g0401051 | 91.128 | 541 | 5 | 2 | 3 | 501 | 59 | 598 | 0.0 | 992 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0837660 | AT4G37670.2 | 64.739 | 536 | 131 | 4 | 16 | 493 | 75 | 610 | 0.0 | 688 |
| Msa0837660 | AT2G22910.1 | 64.326 | 527 | 132 | 3 | 23 | 493 | 75 | 601 | 0.0 | 673 |
| Msa0837660 | AT4G37670.1 | 62.955 | 467 | 115 | 3 | 16 | 424 | 75 | 541 | 0.0 | 575 |
Find 125 sgRNAs with CRISPR-Local
Find 2 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAGCTTGTGCCTTGGCAAT+TGG | 0.248266 | 5_4:-11692659 | Msa0837660:CDS |
| ACAGTAATTCCACTCTTATT+TGG | 0.256128 | 5_4:+11690077 | None:intergenic |
| TCTCCTCAGGGTAAGGAAAA+AGG | 0.290072 | 5_4:+11691035 | None:intergenic |
| CAGCTTGTGCCTTGGCAATT+GGG | 0.298414 | 5_4:-11692658 | Msa0837660:CDS |
| GGTCCAATACTTGATGAAAA+TGG | 0.316810 | 5_4:-11692608 | Msa0837660:CDS |
| AGCGAGCAAGGCTTTGCTAT+TGG | 0.336026 | 5_4:-11692344 | Msa0837660:CDS |
| TTAAAATTGTTATGCTCAAC+AGG | 0.336039 | 5_4:+11692470 | None:intergenic |
| TGAAATATGTGCAGATTATA+TGG | 0.338432 | 5_4:-11690447 | Msa0837660:intron |
| CAAACAACTTATTCAGCCTT+TGG | 0.343314 | 5_4:-11691272 | Msa0837660:CDS |
| TTGGAATTGTTTACAAGAGA+TGG | 0.378945 | 5_4:-11691896 | Msa0837660:CDS |
| TTCGTTCCCTTCGTGAAGCT+TGG | 0.379069 | 5_4:-11694562 | Msa0837660:CDS |
| AGCTGCTATTTCACTTTGCT+CGG | 0.382328 | 5_4:+11692529 | None:intergenic |
| TTGTTGGATGGCACTATTAG+TGG | 0.387463 | 5_4:-11691929 | Msa0837660:CDS |
| GCAGTCAAATCTCCACCTAA+TGG | 0.388073 | 5_4:-11692443 | Msa0837660:CDS |
| TGCCCTTGGCCACGGCAATC+AGG | 0.388955 | 5_4:+11690980 | None:intergenic |
| GGAATTGATTATGGGTCAAC+TGG | 0.390661 | 5_4:-11693083 | Msa0837660:CDS |
| CATATAATACTTAGAATTGC+GGG | 0.398277 | 5_4:+11690112 | None:intergenic |
| TTTGAGAATGGAGATGGCTT+AGG | 0.403018 | 5_4:-11692371 | Msa0837660:CDS |
| TCGAGCTACAAGATTGTTAC+GGG | 0.407532 | 5_4:+11689949 | None:intergenic |
| TTTGGAGGCATGTGGCATAC+TGG | 0.410523 | 5_4:-11691254 | Msa0837660:CDS |
| TGATTGCCGTGGCCAAGGGC+AGG | 0.426100 | 5_4:-11690978 | Msa0837660:CDS |
| ATGACTAAGGTTTGAATTGT+GGG | 0.427626 | 5_4:+11689834 | None:intergenic |
| GCAATTTGTCTCCCTGCCCT+TGG | 0.430775 | 5_4:+11690966 | None:intergenic |
| ACCTCTGCACACTTCTCCTC+AGG | 0.437888 | 5_4:+11691022 | None:intergenic |
| GTGGTCGGTGGAATTGATTA+TGG | 0.441744 | 5_4:-11693092 | Msa0837660:CDS |
| GTTGGATAATTTCGTTGTTG+TGG | 0.449871 | 5_4:-11691089 | Msa0837660:CDS |
| TTCTTTCTCCAGCACCTATG+AGG | 0.453361 | 5_4:-11692687 | Msa0837660:intron |
| ACAAGCTGTTGCAACCTCAT+AGG | 0.454255 | 5_4:+11692673 | None:intergenic |
| ATGTCTACGAATATTGCATA+TGG | 0.454955 | 5_4:+11693310 | None:intergenic |
| TTGTAGCTCGAATCTGTGTT+TGG | 0.456577 | 5_4:-11689937 | Msa0837660:three_prime_UTR |
| ATTCAATACTTCACCAGAGC+TGG | 0.458082 | 5_4:+11692977 | None:intergenic |
| GAACGTCTAAGTCGAATGAA+TGG | 0.458588 | 5_4:-11692314 | Msa0837660:CDS |
| GTGAAAGCGGTTGAGGAAGA+AGG | 0.459644 | 5_4:-11692500 | Msa0837660:CDS |
| CGCATGCGTGAGAGACTTGA+TGG | 0.466167 | 5_4:-11693035 | Msa0837660:CDS |
| GAGAAGAAAGCAGCATCTCT+TGG | 0.466675 | 5_4:-11690425 | Msa0837660:CDS |
| GGTTCAAAGAGTTCACTTGT+TGG | 0.467382 | 5_4:-11691945 | Msa0837660:CDS |
| TTCGAGCTACAAGATTGTTA+CGG | 0.468888 | 5_4:+11689948 | None:intergenic |
| TCTCCTGATTGCCGTGGCCA+AGG | 0.471147 | 5_4:-11690983 | Msa0837660:CDS |
| TTGAGAATGGAGATGGCTTA+GGG | 0.472572 | 5_4:-11692370 | Msa0837660:CDS |
| TCGAATCTGTGTTTGGTACC+AGG | 0.482437 | 5_4:-11689930 | Msa0837660:three_prime_UTR |
| GGAGCAAGGCTAAGTATGTT+GGG | 0.485838 | 5_4:-11693430 | Msa0837660:CDS |
| TAACTATGTGAAAGCGGTTG+AGG | 0.486501 | 5_4:-11692507 | Msa0837660:CDS |
| AAGAGTGGAATTACTGTTGG+TGG | 0.488225 | 5_4:-11690071 | Msa0837660:CDS |
| GAATGGGTACCTGTCAGAAT+TGG | 0.488645 | 5_4:-11692297 | Msa0837660:CDS |
| GGGAGCAAGGCTAAGTATGT+TGG | 0.490609 | 5_4:-11693431 | Msa0837660:CDS |
| TGGTAACTTTCTTGCAGCCA+AGG | 0.491225 | 5_4:-11693243 | Msa0837660:intron |
| AGCTTGTGCCTTGGCAATTG+GGG | 0.494564 | 5_4:-11692657 | Msa0837660:CDS |
| CAAGAGGCAAGAAACGAATC+AGG | 0.494769 | 5_4:+11692582 | None:intergenic |
| GCACACTTCTCCTCAGGGTA+AGG | 0.495453 | 5_4:+11691028 | None:intergenic |
| CATGACTAAGGTTTGAATTG+TGG | 0.496054 | 5_4:+11689833 | None:intergenic |
| AACGCTGCAATGTGTGCAGC+AGG | 0.498191 | 5_4:-11693377 | Msa0837660:CDS |
| AAGTTAATATGCGTTATAGA+TGG | 0.498769 | 5_4:-11692629 | Msa0837660:CDS |
| CAAAGAGTTCACTTGTTGGA+TGG | 0.500446 | 5_4:-11691941 | Msa0837660:CDS |
| ACAACTTATTCAGCCTTTGG+AGG | 0.501598 | 5_4:-11691269 | Msa0837660:CDS |
| CTCCTGATTGCCGTGGCCAA+GGG | 0.501830 | 5_4:-11690982 | Msa0837660:CDS |
| CTACGATAAGCGAGAAGATA+AGG | 0.502922 | 5_4:+11694539 | None:intergenic |
| AACATGTCTGCTTCTTCAAG+AGG | 0.505053 | 5_4:+11692566 | None:intergenic |
| ATTGTTTACAAGAGATGGGA+TGG | 0.511266 | 5_4:-11691891 | Msa0837660:CDS |
| TGTCTCCCTGCCCTTGGCCA+CGG | 0.513144 | 5_4:+11690972 | None:intergenic |
| TTTGAATTGTGGGCAGGACA+AGG | 0.516611 | 5_4:+11689844 | None:intergenic |
| AACGTCTAAGTCGAATGAAT+GGG | 0.522685 | 5_4:-11692313 | Msa0837660:CDS |
| TGGAATTGTTTACAAGAGAT+GGG | 0.523017 | 5_4:-11691895 | Msa0837660:CDS |
| AACTTGGGCTACTCCAGCTC+TGG | 0.523097 | 5_4:-11692990 | Msa0837660:CDS |
| CTGCTGTACGTGTTGTAAGT+AGG | 0.526017 | 5_4:+11690387 | None:intergenic |
| GGATGGGAACAATGGTGGCT+AGG | 0.526598 | 5_4:-11691874 | Msa0837660:intron |
| GGTAAGTGTGATTGGAAGCA+TGG | 0.528087 | 5_4:-11690004 | Msa0837660:three_prime_UTR |
| TTCGTTGTTGTGGAAAGAGA+AGG | 0.528576 | 5_4:-11691079 | Msa0837660:CDS |
| GAGCAAGGCTTTGCTATTGG+AGG | 0.529772 | 5_4:-11692341 | Msa0837660:CDS |
| TGGTCGGTGGAATTGATTAT+GGG | 0.530856 | 5_4:-11693091 | Msa0837660:CDS |
| CTGCAGCAGCCAATTCTGAC+AGG | 0.535179 | 5_4:+11692288 | None:intergenic |
| GATTGCCGTGGCCAAGGGCA+GGG | 0.536027 | 5_4:-11690977 | Msa0837660:CDS |
| CTTGACAGGTTTGTGAGGCG+TGG | 0.536755 | 5_4:-11690194 | Msa0837660:intron |
| CTAAGGTTTGAATTGTGGGC+AGG | 0.541457 | 5_4:+11689838 | None:intergenic |
| ATAGCAGCTAAACTTTCAGT+TGG | 0.541876 | 5_4:-11693338 | Msa0837660:CDS |
| GCAACTTTCCACAATGGTGT+TGG | 0.543734 | 5_4:-11692395 | Msa0837660:CDS |
| TCATATAATACTTAGAATTG+CGG | 0.547939 | 5_4:+11690111 | None:intergenic |
| TTTATAGCTGCTACAAACGT+TGG | 0.550580 | 5_4:-11691107 | Msa0837660:intron |
| CACAATTCAAACCTTAGTCA+TGG | 0.553441 | 5_4:-11689832 | None:intergenic |
| ACTCCTCAACTCCATGACTA+AGG | 0.559553 | 5_4:+11689821 | None:intergenic |
| GGCTTAGGGACTAGCGAGCA+AGG | 0.560101 | 5_4:-11692356 | Msa0837660:CDS |
| TTACAACACGTACAGCAGAT+TGG | 0.561074 | 5_4:-11690382 | Msa0837660:intron |
| GGGCAGGGAGACAAATTGCT+TGG | 0.563989 | 5_4:-11690962 | Msa0837660:intron |
| GTGAACGGAATCACTGAAGA+AGG | 0.566221 | 5_4:-11694605 | Msa0837660:CDS |
| ATGGAAAACGCATTACAGAA+TGG | 0.566481 | 5_4:-11692424 | Msa0837660:CDS |
| TTGTGCCTTGGCAATTGGGG+CGG | 0.568264 | 5_4:-11692654 | Msa0837660:CDS |
| AACTTCTTGACAGGTTTGTG+AGG | 0.570293 | 5_4:-11690199 | Msa0837660:intron |
| AGACATGTTAATTCGCAAGC+GGG | 0.573614 | 5_4:-11692552 | Msa0837660:CDS |
| GAATTGATTATGGGTCAACT+GGG | 0.574055 | 5_4:-11693082 | Msa0837660:CDS |
| GGTTAAGCGGTCTGATGAAG+AGG | 0.575532 | 5_4:-11691233 | Msa0837660:intron |
| TTGAACAACGAAGGAGTGAA+CGG | 0.576908 | 5_4:-11694620 | Msa0837660:CDS |
| GTAAGTGTGATTGGAAGCAT+GGG | 0.577212 | 5_4:-11690003 | Msa0837660:three_prime_UTR |
| CAGAGAAGAGGGGTGGTCGG+TGG | 0.577629 | 5_4:-11693104 | Msa0837660:CDS |
| AGCAGCTAACTATGTGAAAG+CGG | 0.584271 | 5_4:-11692513 | Msa0837660:CDS |
| AATAAGAGTGGAATTACTGT+TGG | 0.587427 | 5_4:-11690074 | Msa0837660:CDS |
| CTTGTCCGCCCCAATTGCCA+AGG | 0.592760 | 5_4:+11692649 | None:intergenic |
| TGACAGAGAAGAGGGGTGGT+CGG | 0.595410 | 5_4:-11693107 | Msa0837660:intron |
| TTGAATTGTGGGCAGGACAA+GGG | 0.600713 | 5_4:+11689845 | None:intergenic |
| ATTCAGCCTTTGGAGGCATG+TGG | 0.604861 | 5_4:-11691262 | Msa0837660:CDS |
| ATATGCAATATTCGTAGACA+TGG | 0.605536 | 5_4:-11693308 | Msa0837660:CDS |
| ACAACAGATTTACAATACAA+AGG | 0.606913 | 5_4:+11689887 | None:intergenic |
| GGTTGCAACAGCTTGTGCCT+TGG | 0.608428 | 5_4:-11692666 | Msa0837660:CDS |
| CTACGAATATTGCATATGGA+AGG | 0.610959 | 5_4:+11693314 | None:intergenic |
| AAGAGATGGGATGGGAACAA+TGG | 0.612647 | 5_4:-11691882 | Msa0837660:CDS |
| TTGTTTACAAGAGATGGGAT+GGG | 0.612686 | 5_4:-11691890 | Msa0837660:CDS |
| CGCAATGCGTTGAACAACGA+AGG | 0.616719 | 5_4:-11694629 | Msa0837660:CDS |
| TACTGCGTTTACAGGGAGCA+AGG | 0.617201 | 5_4:-11693444 | Msa0837660:intron |
| TCTCAAAACCAACACCATTG+TGG | 0.620130 | 5_4:+11692387 | None:intergenic |
| AAGCTTCTACCAAATAAGAG+TGG | 0.625840 | 5_4:-11690086 | Msa0837660:CDS |
| AGATGGGATGGGAACAATGG+TGG | 0.628080 | 5_4:-11691879 | Msa0837660:CDS |
| AGAGCTGGAGTAGCCCAAGT+TGG | 0.630783 | 5_4:+11692992 | None:intergenic |
| AGTATGCCACATGCCTCCAA+AGG | 0.632526 | 5_4:+11691256 | None:intergenic |
| CATGTGGCATACTGGTTAAG+CGG | 0.633218 | 5_4:-11691246 | Msa0837660:CDS |
| CAGACATGTTAATTCGCAAG+CGG | 0.635149 | 5_4:-11692553 | Msa0837660:CDS |
| GCAGTATCTCCTGATTGCCG+TGG | 0.635516 | 5_4:-11690989 | Msa0837660:CDS |
| GAAGGTCAAATAATTGCATG+TGG | 0.638311 | 5_4:-11691061 | Msa0837660:CDS |
| AATGCTACATGTCGAAGACC+TGG | 0.640649 | 5_4:+11689912 | None:intergenic |
| CCTCTGCACACTTCTCCTCA+GGG | 0.642147 | 5_4:+11691023 | None:intergenic |
| GCTGCAATGTGTGCAGCAGG+AGG | 0.644204 | 5_4:-11693374 | Msa0837660:CDS |
| CCCTGAGGAGAAGTGTGCAG+AGG | 0.655036 | 5_4:-11691023 | Msa0837660:CDS |
| GGTCAAATAATTGCATGTGG+TGG | 0.656987 | 5_4:-11691058 | Msa0837660:CDS |
| GTTAGTGTCAGTGTTACAAG+TGG | 0.659841 | 5_4:-11693263 | Msa0837660:CDS |
| CATAATGCAACTTTCCACAA+TGG | 0.674650 | 5_4:-11692401 | Msa0837660:CDS |
| ATAAGGCCAAGCTTCACGAA+GGG | 0.694309 | 5_4:+11694556 | None:intergenic |
| AATTGATTATGGGTCAACTG+GGG | 0.744930 | 5_4:-11693081 | Msa0837660:CDS |
| ATGCGTGAGAGACTTGATGG+TGG | 0.746452 | 5_4:-11693032 | Msa0837660:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTGAACAACGAAGGAGTGAA+CGG | - | chr5_4:11689851-11689870 | Msa0837660:three_prime_UTR | 40.0% | |
| CGCAATGCGTTGAACAACGA+AGG | - | chr5_4:11689842-11689861 | Msa0837660:three_prime_UTR | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 11689833 | 11694660 | 11689833 | ID=Msa0837660;Name=Msa0837660 |
| chr5_4 | mRNA | 11689833 | 11694660 | 11689833 | ID=Msa0837660-mRNA-1;Parent=Msa0837660;Name=Msa0837660-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0.87|0.88|1|1|1|9|216|501 |
| chr5_4 | exon | 11689833 | 11690208 | 11689833 | ID=Msa0837660-mRNA-1:exon:5198;Parent=Msa0837660-mRNA-1 |
| chr5_4 | exon | 11690383 | 11690455 | 11690383 | ID=Msa0837660-mRNA-1:exon:5197;Parent=Msa0837660-mRNA-1 |
| chr5_4 | exon | 11690963 | 11691122 | 11690963 | ID=Msa0837660-mRNA-1:exon:5196;Parent=Msa0837660-mRNA-1 |
| chr5_4 | exon | 11691234 | 11691345 | 11691234 | ID=Msa0837660-mRNA-1:exon:5195;Parent=Msa0837660-mRNA-1 |
| chr5_4 | exon | 11691875 | 11691972 | 11691875 | ID=Msa0837660-mRNA-1:exon:5194;Parent=Msa0837660-mRNA-1 |
| chr5_4 | exon | 11692274 | 11692697 | 11692274 | ID=Msa0837660-mRNA-1:exon:5193;Parent=Msa0837660-mRNA-1 |
| chr5_4 | exon | 11692972 | 11693123 | 11692972 | ID=Msa0837660-mRNA-1:exon:5192;Parent=Msa0837660-mRNA-1 |
| chr5_4 | exon | 11693244 | 11693452 | 11693244 | ID=Msa0837660-mRNA-1:exon:5191;Parent=Msa0837660-mRNA-1 |
| chr5_4 | exon | 11694543 | 11694660 | 11694543 | ID=Msa0837660-mRNA-1:exon:5190;Parent=Msa0837660-mRNA-1 |
| chr5_4 | CDS | 11694543 | 11694660 | 11694543 | ID=Msa0837660-mRNA-1:cds;Parent=Msa0837660-mRNA-1 |
| chr5_4 | CDS | 11693244 | 11693452 | 11693244 | ID=Msa0837660-mRNA-1:cds;Parent=Msa0837660-mRNA-1 |
| chr5_4 | CDS | 11692972 | 11693123 | 11692972 | ID=Msa0837660-mRNA-1:cds;Parent=Msa0837660-mRNA-1 |
| chr5_4 | CDS | 11692274 | 11692697 | 11692274 | ID=Msa0837660-mRNA-1:cds;Parent=Msa0837660-mRNA-1 |
| chr5_4 | CDS | 11691875 | 11691972 | 11691875 | ID=Msa0837660-mRNA-1:cds;Parent=Msa0837660-mRNA-1 |
| chr5_4 | CDS | 11691234 | 11691345 | 11691234 | ID=Msa0837660-mRNA-1:cds;Parent=Msa0837660-mRNA-1 |
| chr5_4 | CDS | 11690963 | 11691122 | 11690963 | ID=Msa0837660-mRNA-1:cds;Parent=Msa0837660-mRNA-1 |
| chr5_4 | CDS | 11690383 | 11690455 | 11690383 | ID=Msa0837660-mRNA-1:cds;Parent=Msa0837660-mRNA-1 |
| chr5_4 | CDS | 11690049 | 11690208 | 11690049 | ID=Msa0837660-mRNA-1:cds;Parent=Msa0837660-mRNA-1 |
| chr5_4 | three_prime_UTR | 11689833 | 11690048 | 11689833 | ID=Msa0837660-mRNA-1:three_prime_utr;Parent=Msa0837660-mRNA-1 |
| Gene Sequence |
| Protein sequence |
