AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0827980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0827980 MtrunA17_Chr6g0449601 97.076 171 5 0 1 171 1683 1853 9.39e-110 343
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0827980 AT3G27670.1 48.214 168 86 1 1 168 1672 1838 1.56e-48 168

Find 50 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATACCTGTGTATTATTAAA+AGG 0.180843 5_3:-69948132 Msa0827980:CDS
AAGATTAAGCTTGTGAAAAT+TGG 0.199638 5_3:-69949178 Msa0827980:five_prime_UTR
CTGCAGGCACTGCAGTTTCT+TGG 0.239370 5_3:-69948426 Msa0827980:intron
TCTTAGCATGAACCTTCTTT+TGG 0.261696 5_3:+69949188 None:intergenic
GTTAAAGAAGTTTGAAGATC+TGG 0.310122 5_3:+69947913 None:intergenic
TTGTTGGTCCACAATCATAA+AGG 0.368455 5_3:+69948371 None:intergenic
TGAATACCCACCTTGTGATT+TGG 0.374877 5_3:+69949096 None:intergenic
TCTGCTTAATTTAACTCTGT+TGG 0.397019 5_3:+69947793 None:intergenic
ATGATTCTCACTTTCATCAA+TGG 0.397060 5_3:+69948185 None:intergenic
TCTTACCACAGAAGGAAAAC+TGG 0.397246 5_3:+69948863 None:intergenic
ATGTGTAATACATTTCAGTT+AGG 0.410269 5_3:-69947698 Msa0827980:three_prime_UTR
GCAGATGGCTCTTATGTTCA+AGG 0.410983 5_3:-69948216 Msa0827980:CDS
TGTTGGTCCACAATCATAAA+GGG 0.431523 5_3:+69948372 None:intergenic
AGGATTCTGGGATGTCCTCT+TGG 0.432338 5_3:-69948967 Msa0827980:CDS
AACTCAACTGGTTTGAGCTA+AGG 0.437976 5_3:+69947890 None:intergenic
AGCTTAAGCTGCTTATCTAA+TGG 0.444960 5_3:+69948102 None:intergenic
CGACAGAACGTATATATGAT+TGG 0.456539 5_3:-69948251 Msa0827980:CDS
TTCTGACTAAGTAACTCAAC+TGG 0.459149 5_3:+69947878 None:intergenic
TTGACACTTGACACTACAAT+AGG 0.460183 5_3:-69948030 Msa0827980:three_prime_UTR
GATCATTTAGTACCGTTTGT+TGG 0.466568 5_3:+69948355 None:intergenic
TTCTATTGAAGTCCAAAAGA+AGG 0.467092 5_3:-69949209 Msa0827980:five_prime_UTR
TGATTTGGAGTTCAATATGT+AGG 0.469868 5_3:+69949111 None:intergenic
ACTCAACTGGTTTGAGCTAA+GGG 0.473088 5_3:+69947891 None:intergenic
ATATATGCCCTTTATGATTG+TGG 0.478176 5_3:-69948379 Msa0827980:CDS
ATACCTGTGTATTATTAAAA+GGG 0.483637 5_3:-69948131 Msa0827980:CDS
TCTCTTCCACCAACTGAACT+AGG 0.491381 5_3:-69949154 Msa0827980:five_prime_UTR
CTCGAGTTTCTTACCACAGA+AGG 0.492277 5_3:+69948855 None:intergenic
AGTTGTAATACACTCAATAG+TGG 0.496725 5_3:-69947856 Msa0827980:three_prime_UTR
TATGATTGTGGACCAACAAA+CGG 0.502944 5_3:-69948367 Msa0827980:CDS
CTTTCAAGCAACAACCATGC+TGG 0.512593 5_3:+69948077 None:intergenic
AAAAGAGACACAAGGGTGAC+TGG 0.514144 5_3:+69948330 None:intergenic
TGATGAAAGTGAGAATCATA+TGG 0.518457 5_3:-69948181 Msa0827980:CDS
GTTGTAATACACTCAATAGT+GGG 0.520257 5_3:-69947855 Msa0827980:three_prime_UTR
GCAGCTTAAGCTTGCCAGCA+TGG 0.521799 5_3:-69948091 Msa0827980:CDS
TTCTGTCGATGAAAAGAGAT+GGG 0.543675 5_3:+69948266 None:intergenic
AAAGGGCATATATTTGCAGC+AGG 0.551031 5_3:+69948389 None:intergenic
GTTCTGTCGATGAAAAGAGA+TGG 0.566137 5_3:+69948265 None:intergenic
CTGTACATTAGTGCTACCCT+TGG 0.592413 5_3:-69947735 Msa0827980:three_prime_UTR
TGGACAATGCACATAGCAGA+TGG 0.614143 5_3:-69948231 Msa0827980:CDS
TATCTACAACTGCTTCAGCT+CGG 0.614709 5_3:+69947831 None:intergenic
TCAAGAGTTTCAATAAGCCA+CGG 0.616357 5_3:+69948900 None:intergenic
TATCGGCTAAAAGAGACACA+AGG 0.621421 5_3:+69948322 None:intergenic
GGATGTCCTCTTGGAAGTTG+CGG 0.626881 5_3:-69948958 Msa0827980:CDS
GCTTGTCTAAGAAAACAGAA+TGG 0.637205 5_3:+69947757 None:intergenic
AATGTAAAGCATCACAAACG+TGG 0.649517 5_3:-69947991 Msa0827980:three_prime_UTR
ATCGGCTAAAAGAGACACAA+GGG 0.663048 5_3:+69948323 None:intergenic
AAACAGCCGCAACTTCCAAG+AGG 0.669482 5_3:+69948952 None:intergenic
AGATAAGTTTCAAAAGACCG+TGG 0.693472 5_3:-69948917 Msa0827980:CDS
TTGTAATACACTCAATAGTG+GGG 0.693516 5_3:-69947854 Msa0827980:three_prime_UTR
ATATTGAACTCCAAATCACA+AGG 0.704962 5_3:-69949115 Msa0827980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCAAATAAATGAAATAT+CGG + chr5_3:69948158-69948177 None:intergenic 10.0%
!! AAAAAAAACAAAAAAACTTT+AGG - chr5_3:69948267-69948286 Msa0827980:CDS 10.0%
!! AAAAAAACAAAAAAACTTTA+GGG - chr5_3:69948268-69948287 Msa0827980:CDS 10.0%
!! CAAATTTCATATATATATTA+AGG - chr5_3:69948301-69948320 Msa0827980:CDS 10.0%
!!! TTTTCAAATAAATGAAATAT+CGG + chr5_3:69948158-69948177 None:intergenic 10.0%
!! AAAAAAAACAAAAAAACTTT+AGG - chr5_3:69948267-69948286 Msa0827980:CDS 10.0%
!! AAAAAAACAAAAAAACTTTA+GGG - chr5_3:69948268-69948287 Msa0827980:CDS 10.0%
!! CAAATTTCATATATATATTA+AGG - chr5_3:69948301-69948320 Msa0827980:CDS 10.0%
!! ATACCTTTATTAAAACTTAT+TGG - chr5_3:69948048-69948067 Msa0827980:three_prime_UTR 15.0%
!! TACCTTTATTAAAACTTATT+GGG - chr5_3:69948049-69948068 Msa0827980:three_prime_UTR 15.0%
!!! ATTTTAAAGCAAAATTATCT+TGG - chr5_3:69948232-69948251 Msa0827980:CDS 15.0%
!! TTAAAAAAAAATTTAGTTGG+TGG + chr5_3:69948928-69948947 None:intergenic 15.0%
!! CGTTTAAAAAAAAATTTAGT+TGG + chr5_3:69948931-69948950 None:intergenic 15.0%
!!! ACTAAATTTTTTTTTAAACG+TGG - chr5_3:69948931-69948950 Msa0827980:CDS 15.0%
!!! CTAAATTTTTTTTTAAACGT+GGG - chr5_3:69948932-69948951 Msa0827980:CDS 15.0%
!!! TAAATTTTTTTTTAAACGTG+GGG - chr5_3:69948933-69948952 Msa0827980:CDS 15.0%
!! ATACCTTTATTAAAACTTAT+TGG - chr5_3:69948048-69948067 Msa0827980:three_prime_UTR 15.0%
!! TACCTTTATTAAAACTTATT+GGG - chr5_3:69948049-69948068 Msa0827980:three_prime_UTR 15.0%
!!! ATTTTAAAGCAAAATTATCT+TGG - chr5_3:69948232-69948251 Msa0827980:CDS 15.0%
!! TTAAAAAAAAATTTAGTTGG+TGG + chr5_3:69948928-69948947 None:intergenic 15.0%
!! CGTTTAAAAAAAAATTTAGT+TGG + chr5_3:69948931-69948950 None:intergenic 15.0%
!!! ACTAAATTTTTTTTTAAACG+TGG - chr5_3:69948931-69948950 Msa0827980:CDS 15.0%
!!! CTAAATTTTTTTTTAAACGT+GGG - chr5_3:69948932-69948951 Msa0827980:CDS 15.0%
!!! TAAATTTTTTTTTAAACGTG+GGG - chr5_3:69948933-69948952 Msa0827980:CDS 15.0%
!! AATTTAGAAATATATTGAGC+TGG - chr5_3:69947856-69947875 Msa0827980:three_prime_UTR 20.0%
!!! TACCCAATAAGTTTTAATAA+AGG + chr5_3:69948054-69948073 None:intergenic 20.0%
!!! TGCTGACAAATTTTTTAAAT+TGG + chr5_3:69948094-69948113 None:intergenic 20.0%
!! ATACCTGTGTATTATTAAAA+GGG - chr5_3:69948745-69948764 Msa0827980:intron 20.0%
!! AATTTAGAAATATATTGAGC+TGG - chr5_3:69947856-69947875 Msa0827980:three_prime_UTR 20.0%
!!! TACCCAATAAGTTTTAATAA+AGG + chr5_3:69948054-69948073 None:intergenic 20.0%
!!! TGCTGACAAATTTTTTAAAT+TGG + chr5_3:69948094-69948113 None:intergenic 20.0%
!! ATACCTGTGTATTATTAAAA+GGG - chr5_3:69948745-69948764 Msa0827980:intron 20.0%
! AAGATTAAGCTTGTGAAAAT+TGG - chr5_3:69947707-69947726 Msa0827980:three_prime_UTR 25.0%
!! GCTAAATATTAACTTTTTGC+AGG - chr5_3:69947889-69947908 Msa0827980:three_prime_UTR 25.0%
! CTTATTGGGTAGTATTTATT+AGG - chr5_3:69948063-69948082 Msa0827980:exon 25.0%
! AAAATTTGTCAGCAAGAATT+TGG - chr5_3:69948100-69948119 Msa0827980:CDS 25.0%
! CATACCTGTGTATTATTAAA+AGG - chr5_3:69948744-69948763 Msa0827980:intron 25.0%
! ATGTGTAATACATTTCAGTT+AGG - chr5_3:69949178-69949197 Msa0827980:five_prime_UTR 25.0%
! AAGATTAAGCTTGTGAAAAT+TGG - chr5_3:69947707-69947726 Msa0827980:three_prime_UTR 25.0%
!! GCTAAATATTAACTTTTTGC+AGG - chr5_3:69947889-69947908 Msa0827980:three_prime_UTR 25.0%
! CTTATTGGGTAGTATTTATT+AGG - chr5_3:69948063-69948082 Msa0827980:exon 25.0%
! AAAATTTGTCAGCAAGAATT+TGG - chr5_3:69948100-69948119 Msa0827980:CDS 25.0%
! CATACCTGTGTATTATTAAA+AGG - chr5_3:69948744-69948763 Msa0827980:intron 25.0%
! ATGTGTAATACATTTCAGTT+AGG - chr5_3:69949178-69949197 Msa0827980:five_prime_UTR 25.0%
TTCTATTGAAGTCCAAAAGA+AGG - chr5_3:69947676-69947695 Msa0827980:three_prime_UTR 30.0%
!! TTCATTTTTCCTAGTTCAGT+TGG + chr5_3:69947743-69947762 None:intergenic 30.0%
! TGATTTGGAGTTCAATATGT+AGG + chr5_3:69947768-69947787 None:intergenic 30.0%
ATATTGAACTCCAAATCACA+AGG - chr5_3:69947770-69947789 Msa0827980:three_prime_UTR 30.0%
! ATTAACTTTTTGCAGGATTC+TGG - chr5_3:69947896-69947915 Msa0827980:three_prime_UTR 30.0%
! TTAACTTTTTGCAGGATTCT+GGG - chr5_3:69947897-69947916 Msa0827980:three_prime_UTR 30.0%
ATTGAAGAGAGTAGTACTTT+AGG - chr5_3:69948329-69948348 Msa0827980:CDS 30.0%
TTGAAGAGAGTAGTACTTTA+GGG - chr5_3:69948330-69948349 Msa0827980:CDS 30.0%
ATATATGCCCTTTATGATTG+TGG - chr5_3:69948497-69948516 Msa0827980:intron 30.0%
! AACAGTGTTCCAGTTTTTAT+CGG + chr5_3:69948574-69948593 None:intergenic 30.0%
ATGATTCTCACTTTCATCAA+TGG + chr5_3:69948694-69948713 None:intergenic 30.0%
TGATGAAAGTGAGAATCATA+TGG - chr5_3:69948695-69948714 Msa0827980:intron 30.0%
! GTACCCTTTTAATAATACAC+AGG + chr5_3:69948751-69948770 None:intergenic 30.0%
GTTAAAGAAGTTTGAAGATC+TGG + chr5_3:69948966-69948985 None:intergenic 30.0%
AGTTGTAATACACTCAATAG+TGG - chr5_3:69949020-69949039 Msa0827980:intron 30.0%
GTTGTAATACACTCAATAGT+GGG - chr5_3:69949021-69949040 Msa0827980:intron 30.0%
TTGTAATACACTCAATAGTG+GGG - chr5_3:69949022-69949041 Msa0827980:intron 30.0%
TCTGCTTAATTTAACTCTGT+TGG + chr5_3:69949086-69949105 None:intergenic 30.0%
! ATTTTTGCAAGTAATGCCAA+GGG + chr5_3:69949160-69949179 None:intergenic 30.0%
TCTTGATTTCAGAGTTGAAT+AGG - chr5_3:69949240-69949259 Msa0827980:five_prime_UTR 30.0%
TTCTATTGAAGTCCAAAAGA+AGG - chr5_3:69947676-69947695 Msa0827980:three_prime_UTR 30.0%
!! TTCATTTTTCCTAGTTCAGT+TGG + chr5_3:69947743-69947762 None:intergenic 30.0%
! TGATTTGGAGTTCAATATGT+AGG + chr5_3:69947768-69947787 None:intergenic 30.0%
ATATTGAACTCCAAATCACA+AGG - chr5_3:69947770-69947789 Msa0827980:three_prime_UTR 30.0%
! ATTAACTTTTTGCAGGATTC+TGG - chr5_3:69947896-69947915 Msa0827980:three_prime_UTR 30.0%
! TTAACTTTTTGCAGGATTCT+GGG - chr5_3:69947897-69947916 Msa0827980:three_prime_UTR 30.0%
ATTGAAGAGAGTAGTACTTT+AGG - chr5_3:69948329-69948348 Msa0827980:CDS 30.0%
TTGAAGAGAGTAGTACTTTA+GGG - chr5_3:69948330-69948349 Msa0827980:CDS 30.0%
ATATATGCCCTTTATGATTG+TGG - chr5_3:69948497-69948516 Msa0827980:intron 30.0%
! AACAGTGTTCCAGTTTTTAT+CGG + chr5_3:69948574-69948593 None:intergenic 30.0%
ATGATTCTCACTTTCATCAA+TGG + chr5_3:69948694-69948713 None:intergenic 30.0%
TGATGAAAGTGAGAATCATA+TGG - chr5_3:69948695-69948714 Msa0827980:intron 30.0%
! GTACCCTTTTAATAATACAC+AGG + chr5_3:69948751-69948770 None:intergenic 30.0%
GTTAAAGAAGTTTGAAGATC+TGG + chr5_3:69948966-69948985 None:intergenic 30.0%
AGTTGTAATACACTCAATAG+TGG - chr5_3:69949020-69949039 Msa0827980:intron 30.0%
GTTGTAATACACTCAATAGT+GGG - chr5_3:69949021-69949040 Msa0827980:intron 30.0%
TTGTAATACACTCAATAGTG+GGG - chr5_3:69949022-69949041 Msa0827980:intron 30.0%
TCTGCTTAATTTAACTCTGT+TGG + chr5_3:69949086-69949105 None:intergenic 30.0%
! ATTTTTGCAAGTAATGCCAA+GGG + chr5_3:69949160-69949179 None:intergenic 30.0%
! TCTTAGCATGAACCTTCTTT+TGG + chr5_3:69947691-69947710 None:intergenic 35.0%
! ATTTTTCCTAGTTCAGTTGG+TGG + chr5_3:69947740-69947759 None:intergenic 35.0%
CACAGTAACTTGGATTGATT+GGG - chr5_3:69947809-69947828 Msa0827980:three_prime_UTR 35.0%
AGATAAGTTTCAAAAGACCG+TGG - chr5_3:69947959-69947978 Msa0827980:three_prime_UTR 35.0%
TCAAGAGTTTCAATAAGCCA+CGG + chr5_3:69947979-69947998 None:intergenic 35.0%
! TACTTTAGGGACTACATTGA+TGG - chr5_3:69948343-69948362 Msa0827980:CDS 35.0%
GGTCAATAACAAATTCAGCT+TGG + chr5_3:69948398-69948417 None:intergenic 35.0%
AGATGGATATAAAGTGAGCA+AGG + chr5_3:69948419-69948438 None:intergenic 35.0%
TGTTGGTCCACAATCATAAA+GGG + chr5_3:69948507-69948526 None:intergenic 35.0%
TTGTTGGTCCACAATCATAA+AGG + chr5_3:69948508-69948527 None:intergenic 35.0%
TATGATTGTGGACCAACAAA+CGG - chr5_3:69948509-69948528 Msa0827980:intron 35.0%
GATCATTTAGTACCGTTTGT+TGG + chr5_3:69948524-69948543 None:intergenic 35.0%
! CTCTTTTAGCCGATAAAAAC+TGG - chr5_3:69948562-69948581 Msa0827980:intron 35.0%
TTCTGTCGATGAAAAGAGAT+GGG + chr5_3:69948613-69948632 None:intergenic 35.0%
CGACAGAACGTATATATGAT+TGG - chr5_3:69948625-69948644 Msa0827980:intron 35.0%
AGCTTAAGCTGCTTATCTAA+TGG + chr5_3:69948777-69948796 None:intergenic 35.0%
TTGACACTTGACACTACAAT+AGG - chr5_3:69948846-69948865 Msa0827980:intron 35.0%
! AATGTAAAGCATCACAAACG+TGG - chr5_3:69948885-69948904 Msa0827980:CDS 35.0%
TTCTGACTAAGTAACTCAAC+TGG + chr5_3:69949001-69949020 None:intergenic 35.0%
GCTTGTCTAAGAAAACAGAA+TGG + chr5_3:69949122-69949141 None:intergenic 35.0%
! CATTTTTGCAAGTAATGCCA+AGG + chr5_3:69949161-69949180 None:intergenic 35.0%
! TCTTAGCATGAACCTTCTTT+TGG + chr5_3:69947691-69947710 None:intergenic 35.0%
! ATTTTTCCTAGTTCAGTTGG+TGG + chr5_3:69947740-69947759 None:intergenic 35.0%
CACAGTAACTTGGATTGATT+GGG - chr5_3:69947809-69947828 Msa0827980:three_prime_UTR 35.0%
AGATAAGTTTCAAAAGACCG+TGG - chr5_3:69947959-69947978 Msa0827980:three_prime_UTR 35.0%
TCAAGAGTTTCAATAAGCCA+CGG + chr5_3:69947979-69947998 None:intergenic 35.0%
! TACTTTAGGGACTACATTGA+TGG - chr5_3:69948343-69948362 Msa0827980:CDS 35.0%
GGTCAATAACAAATTCAGCT+TGG + chr5_3:69948398-69948417 None:intergenic 35.0%
AGATGGATATAAAGTGAGCA+AGG + chr5_3:69948419-69948438 None:intergenic 35.0%
TGTTGGTCCACAATCATAAA+GGG + chr5_3:69948507-69948526 None:intergenic 35.0%
TTGTTGGTCCACAATCATAA+AGG + chr5_3:69948508-69948527 None:intergenic 35.0%
TATGATTGTGGACCAACAAA+CGG - chr5_3:69948509-69948528 Msa0827980:intron 35.0%
GATCATTTAGTACCGTTTGT+TGG + chr5_3:69948524-69948543 None:intergenic 35.0%
! CTCTTTTAGCCGATAAAAAC+TGG - chr5_3:69948562-69948581 Msa0827980:intron 35.0%
TTCTGTCGATGAAAAGAGAT+GGG + chr5_3:69948613-69948632 None:intergenic 35.0%
CGACAGAACGTATATATGAT+TGG - chr5_3:69948625-69948644 Msa0827980:intron 35.0%
AGCTTAAGCTGCTTATCTAA+TGG + chr5_3:69948777-69948796 None:intergenic 35.0%
TTGACACTTGACACTACAAT+AGG - chr5_3:69948846-69948865 Msa0827980:intron 35.0%
! AATGTAAAGCATCACAAACG+TGG - chr5_3:69948885-69948904 Msa0827980:CDS 35.0%
TTCTGACTAAGTAACTCAAC+TGG + chr5_3:69949001-69949020 None:intergenic 35.0%
GCTTGTCTAAGAAAACAGAA+TGG + chr5_3:69949122-69949141 None:intergenic 35.0%
! CATTTTTGCAAGTAATGCCA+AGG + chr5_3:69949161-69949180 None:intergenic 35.0%
TTGAACTCCAAATCACAAGG+TGG - chr5_3:69947773-69947792 Msa0827980:three_prime_UTR 40.0%
TGAACTCCAAATCACAAGGT+GGG - chr5_3:69947774-69947793 Msa0827980:three_prime_UTR 40.0%
TGAATACCCACCTTGTGATT+TGG + chr5_3:69947783-69947802 None:intergenic 40.0%
GCACAGTAACTTGGATTGAT+TGG - chr5_3:69947808-69947827 Msa0827980:three_prime_UTR 40.0%
! TGTATCCAGTTTTCCTTCTG+TGG - chr5_3:69948008-69948027 Msa0827980:three_prime_UTR 40.0%
TCTTACCACAGAAGGAAAAC+TGG + chr5_3:69948016-69948035 None:intergenic 40.0%
! CTGCAGAATAATGGAAGAGA+TGG + chr5_3:69948436-69948455 None:intergenic 40.0%
CATCTCTTCCATTATTCTGC+AGG - chr5_3:69948434-69948453 Msa0827980:intron 40.0%
! AAAGGGCATATATTTGCAGC+AGG + chr5_3:69948490-69948509 None:intergenic 40.0%
ATCGGCTAAAAGAGACACAA+GGG + chr5_3:69948556-69948575 None:intergenic 40.0%
! TATCGGCTAAAAGAGACACA+AGG + chr5_3:69948557-69948576 None:intergenic 40.0%
GTTCTGTCGATGAAAAGAGA+TGG + chr5_3:69948614-69948633 None:intergenic 40.0%
!! ACTCAACTGGTTTGAGCTAA+GGG + chr5_3:69948988-69949007 None:intergenic 40.0%
!! AACTCAACTGGTTTGAGCTA+AGG + chr5_3:69948989-69949008 None:intergenic 40.0%
TATCTACAACTGCTTCAGCT+CGG + chr5_3:69949048-69949067 None:intergenic 40.0%
TTGAACTCCAAATCACAAGG+TGG - chr5_3:69947773-69947792 Msa0827980:three_prime_UTR 40.0%
TGAACTCCAAATCACAAGGT+GGG - chr5_3:69947774-69947793 Msa0827980:three_prime_UTR 40.0%
TGAATACCCACCTTGTGATT+TGG + chr5_3:69947783-69947802 None:intergenic 40.0%
GCACAGTAACTTGGATTGAT+TGG - chr5_3:69947808-69947827 Msa0827980:three_prime_UTR 40.0%
! TGTATCCAGTTTTCCTTCTG+TGG - chr5_3:69948008-69948027 Msa0827980:three_prime_UTR 40.0%
TCTTACCACAGAAGGAAAAC+TGG + chr5_3:69948016-69948035 None:intergenic 40.0%
! CTGCAGAATAATGGAAGAGA+TGG + chr5_3:69948436-69948455 None:intergenic 40.0%
CATCTCTTCCATTATTCTGC+AGG - chr5_3:69948434-69948453 Msa0827980:intron 40.0%
! AAAGGGCATATATTTGCAGC+AGG + chr5_3:69948490-69948509 None:intergenic 40.0%
ATCGGCTAAAAGAGACACAA+GGG + chr5_3:69948556-69948575 None:intergenic 40.0%
! TATCGGCTAAAAGAGACACA+AGG + chr5_3:69948557-69948576 None:intergenic 40.0%
GTTCTGTCGATGAAAAGAGA+TGG + chr5_3:69948614-69948633 None:intergenic 40.0%
!! ACTCAACTGGTTTGAGCTAA+GGG + chr5_3:69948988-69949007 None:intergenic 40.0%
!! AACTCAACTGGTTTGAGCTA+AGG + chr5_3:69948989-69949008 None:intergenic 40.0%
TATCTACAACTGCTTCAGCT+CGG + chr5_3:69949048-69949067 None:intergenic 40.0%
TCTCTTCCACCAACTGAACT+AGG - chr5_3:69947731-69947750 Msa0827980:three_prime_UTR 45.0%
TTCAGCTCTGCACAGTAACT+TGG - chr5_3:69947799-69947818 Msa0827980:three_prime_UTR 45.0%
CTCGAGTTTCTTACCACAGA+AGG + chr5_3:69948024-69948043 None:intergenic 45.0%
AAAAGAGACACAAGGGTGAC+TGG + chr5_3:69948549-69948568 None:intergenic 45.0%
TGGACAATGCACATAGCAGA+TGG - chr5_3:69948645-69948664 Msa0827980:intron 45.0%
GCAGATGGCTCTTATGTTCA+AGG - chr5_3:69948660-69948679 Msa0827980:intron 45.0%
CTTTCAAGCAACAACCATGC+TGG + chr5_3:69948802-69948821 None:intergenic 45.0%
CTGTACATTAGTGCTACCCT+TGG - chr5_3:69949141-69949160 Msa0827980:five_prime_UTR 45.0%
TCTCTTCCACCAACTGAACT+AGG - chr5_3:69947731-69947750 Msa0827980:three_prime_UTR 45.0%
TTCAGCTCTGCACAGTAACT+TGG - chr5_3:69947799-69947818 Msa0827980:three_prime_UTR 45.0%
CTCGAGTTTCTTACCACAGA+AGG + chr5_3:69948024-69948043 None:intergenic 45.0%
AAAAGAGACACAAGGGTGAC+TGG + chr5_3:69948549-69948568 None:intergenic 45.0%
TGGACAATGCACATAGCAGA+TGG - chr5_3:69948645-69948664 Msa0827980:intron 45.0%
GCAGATGGCTCTTATGTTCA+AGG - chr5_3:69948660-69948679 Msa0827980:intron 45.0%
CTTTCAAGCAACAACCATGC+TGG + chr5_3:69948802-69948821 None:intergenic 45.0%
CTGTACATTAGTGCTACCCT+TGG - chr5_3:69949141-69949160 Msa0827980:five_prime_UTR 45.0%
!! AAATTTCATATATATATTAA+GGG - chr5_3:69948302-69948321 Msa0827980:CDS 5.0%
!! AAATTTCATATATATATTAA+GGG - chr5_3:69948302-69948321 Msa0827980:CDS 5.0%
!! AGGATTCTGGGATGTCCTCT+TGG - chr5_3:69947909-69947928 Msa0827980:three_prime_UTR 50.0%
GGATGTCCTCTTGGAAGTTG+CGG - chr5_3:69947918-69947937 Msa0827980:three_prime_UTR 50.0%
AAACAGCCGCAACTTCCAAG+AGG + chr5_3:69947927-69947946 None:intergenic 50.0%
CTGCAGTGCCTGCAGAATAA+TGG + chr5_3:69948445-69948464 None:intergenic 50.0%
!! AGGATTCTGGGATGTCCTCT+TGG - chr5_3:69947909-69947928 Msa0827980:three_prime_UTR 50.0%
GGATGTCCTCTTGGAAGTTG+CGG - chr5_3:69947918-69947937 Msa0827980:three_prime_UTR 50.0%
AAACAGCCGCAACTTCCAAG+AGG + chr5_3:69947927-69947946 None:intergenic 50.0%
CTGCAGTGCCTGCAGAATAA+TGG + chr5_3:69948445-69948464 None:intergenic 50.0%
! CTGCAGGCACTGCAGTTTCT+TGG - chr5_3:69948450-69948469 Msa0827980:intron 55.0%
GCAGCTTAAGCTTGCCAGCA+TGG - chr5_3:69948785-69948804 Msa0827980:intron 55.0%
! CTGCAGGCACTGCAGTTTCT+TGG - chr5_3:69948450-69948469 Msa0827980:intron 55.0%
GCAGCTTAAGCTTGCCAGCA+TGG - chr5_3:69948785-69948804 Msa0827980:intron 55.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 69947639 69949259 69947639 ID=Msa0827980;Name=Msa0827980
chr5_3 mRNA 69947639 69949259 69947639 ID=Msa0827980-mRNA-1;Parent=Msa0827980;Name=Msa0827980-mRNA-1;_AED=0.00;_eAED=0.00;_QI=119|1|1|1|0.5|0.66|3|441|171
chr5_3 exon 69947639 69948442 69947639 ID=Msa0827980-mRNA-1:exon:21931;Parent=Msa0827980-mRNA-1
chr5_3 exon 69948869 69948987 69948869 ID=Msa0827980-mRNA-1:exon:21930;Parent=Msa0827980-mRNA-1
chr5_3 exon 69949107 69949259 69949107 ID=Msa0827980-mRNA-1:exon:21929;Parent=Msa0827980-mRNA-1
chr5_3 five_prime_UTR 69949141 69949259 69949141 ID=Msa0827980-mRNA-1:five_prime_utr;Parent=Msa0827980-mRNA-1
chr5_3 CDS 69949107 69949140 69949107 ID=Msa0827980-mRNA-1:cds;Parent=Msa0827980-mRNA-1
chr5_3 CDS 69948869 69948987 69948869 ID=Msa0827980-mRNA-1:cds;Parent=Msa0827980-mRNA-1
chr5_3 CDS 69948080 69948442 69948080 ID=Msa0827980-mRNA-1:cds;Parent=Msa0827980-mRNA-1
chr5_3 three_prime_UTR 69947639 69948079 69947639 ID=Msa0827980-mRNA-1:three_prime_utr;Parent=Msa0827980-mRNA-1
Gene Sequence

>Msa0827980

ATGAAATCCTACATATTGAACTCCAAATCACAAGGATTCTGGGATGTCCTCTTGGAAGTTGCGGCTGTTTTATATCATGCAGAGATAAGTTTCAAAAGACCGTGGCTTATTGAAACTCTTGAAATTAGCTGTGTATCCAGTTTTCCTTCTGTGGCACTGCAGTTTCTTGGTTTGCTATCTGCTACCTGCTGCAAATATATGCCCTTTATGATTGTGGACCAACAAACGGTACTAAATGATCTACCAGTCACCCTTGTGTCTCTTTTAGCCGATAAAAACTGGAACACTGTTGCAGAAACTGTAGTTTCCCATCTCTTTTCATCGACAGAACGTATATATGATTGGACAATGCACATAGCAGATGGCTCTTATGTTCAAGGTTCGCAAACCATTGATGAAAGTGAGAATCATATGGCTACTTTTCTACTGCAAGTGATGTATCATACCTGTGTATTATTAAAAGGGTACTTGCCATTAGATAAGCAGCTTAAGCTTGCCAGCATGGTTGTTGCTTGA
Protein sequence

>Msa0827980

MKSYILNSKSQGFWDVLLEVAAVLYHAEISFKRPWLIETLEISCVSSFPSVALQFLGLLSATCCKYMPFMIVDQQTVLNDLPVTLVSLLADKNWNTVAETVVSHLFSSTERIYDWTMHIADGSYVQGSQTIDESENHMATFLLQVMYHTCVLLKGYLPLDKQLKLASMVVA*