Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0942210 | XP_003618787.1 | 99.257 | 404 | 3 | 0 | 1 | 404 | 1 | 404 | 0.0 | 835 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0942210 | sp|Q93Z25|PLY22_ARATH | 73.020 | 404 | 106 | 2 | 4 | 404 | 29 | 432 | 0.0 | 616 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0942210 | G7KN73 | 99.257 | 404 | 3 | 0 | 1 | 404 | 1 | 404 | 0.0 | 835 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0044160 | Msa0942210 | 0.814755 | 1.311006e-51 | -8.615850e-47 |
| Msa0605560 | Msa0942210 | 0.814676 | 1.364903e-51 | -8.615850e-47 |
| Msa0657720 | Msa0942210 | 0.804328 | 2.277240e-49 | -8.615850e-47 |
| Msa0714640 | Msa0942210 | 0.806722 | 7.164946e-50 | -8.615850e-47 |
| Msa0722890 | Msa0942210 | 0.825207 | 5.316592e-54 | -8.615850e-47 |
| Msa0723090 | Msa0942210 | 0.858977 | 5.687780e-63 | -8.615850e-47 |
| Msa0226310 | Msa0942210 | 0.804389 | 2.211294e-49 | -8.615850e-47 |
| Msa0437520 | Msa0942210 | 0.808639 | 2.805941e-50 | -8.615850e-47 |
| Msa0304570 | Msa0942210 | 0.818266 | 2.143915e-52 | -8.615850e-47 |
| Msa0304680 | Msa0942210 | 0.830697 | 2.537306e-55 | -8.615850e-47 |
| Msa0330520 | Msa0942210 | 0.805649 | 1.205516e-49 | -8.615850e-47 |
| Msa0528570 | Msa0942210 | 0.804605 | 1.993888e-49 | -8.615850e-47 |
| Msa0876930 | Msa0942210 | 0.862828 | 3.844513e-64 | -8.615850e-47 |
| Msa0876940 | Msa0942210 | 0.919365 | 4.930245e-87 | -8.615850e-47 |
| Msa0942210 | Msa0942220 | 0.946854 | 2.080789e-105 | -8.615850e-47 |
| Msa0942210 | Msa0979180 | 0.849902 | 2.398923e-60 | -8.615850e-47 |
| Msa0942210 | Msa1415540 | 0.804797 | 1.817825e-49 | -8.615850e-47 |
| Msa0942210 | Msa1449900 | 0.812782 | 3.565422e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0942210 | MtrunA17_Chr6g0458201 | 99.257 | 404 | 3 | 0 | 1 | 404 | 1 | 404 | 0.0 | 835 |
| Msa0942210 | MtrunA17_Chr4g0058051 | 80.890 | 382 | 72 | 1 | 24 | 404 | 26 | 407 | 0.0 | 638 |
| Msa0942210 | MtrunA17_Chr3g0116521 | 75.682 | 403 | 98 | 0 | 2 | 404 | 4 | 406 | 0.0 | 620 |
| Msa0942210 | MtrunA17_Chr3g0123321 | 75.192 | 391 | 94 | 2 | 14 | 404 | 16 | 403 | 0.0 | 587 |
| Msa0942210 | MtrunA17_Chr1g0169731 | 67.885 | 383 | 123 | 0 | 22 | 404 | 68 | 450 | 0.0 | 565 |
| Msa0942210 | MtrunA17_Chr7g0237761 | 65.803 | 386 | 132 | 0 | 19 | 404 | 59 | 444 | 0.0 | 550 |
| Msa0942210 | MtrunA17_Chr3g0123331 | 66.093 | 407 | 134 | 4 | 1 | 404 | 1 | 406 | 0.0 | 533 |
| Msa0942210 | MtrunA17_Chr1g0210321 | 60.775 | 413 | 151 | 3 | 1 | 402 | 1 | 413 | 0.0 | 517 |
| Msa0942210 | MtrunA17_Chr1g0180381 | 62.500 | 384 | 137 | 1 | 26 | 402 | 34 | 417 | 0.0 | 515 |
| Msa0942210 | MtrunA17_Chr7g0264891 | 63.492 | 378 | 137 | 1 | 26 | 402 | 35 | 412 | 3.12e-178 | 504 |
| Msa0942210 | MtrunA17_Chr4g0060211 | 57.487 | 374 | 154 | 3 | 34 | 404 | 63 | 434 | 2.32e-160 | 458 |
| Msa0942210 | MtrunA17_Chr5g0416061 | 56.250 | 368 | 160 | 1 | 38 | 404 | 82 | 449 | 1.20e-153 | 441 |
| Msa0942210 | MtrunA17_Chr8g0362561 | 53.616 | 401 | 170 | 4 | 20 | 404 | 55 | 455 | 3.31e-152 | 438 |
| Msa0942210 | MtrunA17_Chr4g0075231 | 60.571 | 350 | 134 | 3 | 56 | 404 | 39 | 385 | 5.18e-151 | 432 |
| Msa0942210 | MtrunA17_Chr5g0416041 | 55.928 | 388 | 162 | 3 | 26 | 404 | 62 | 449 | 2.12e-150 | 433 |
| Msa0942210 | MtrunA17_Chr6g0452861 | 49.548 | 442 | 184 | 6 | 2 | 404 | 12 | 453 | 5.38e-147 | 424 |
| Msa0942210 | MtrunA17_Chr2g0288921 | 58.857 | 350 | 140 | 3 | 56 | 404 | 63 | 409 | 5.42e-146 | 421 |
| Msa0942210 | MtrunA17_Chr7g0216581 | 58.192 | 354 | 147 | 1 | 52 | 404 | 98 | 451 | 3.06e-143 | 415 |
| Msa0942210 | MtrunA17_Chr8g0373761 | 45.480 | 354 | 185 | 6 | 56 | 404 | 42 | 392 | 4.24e-99 | 300 |
| Msa0942210 | MtrunA17_Chr3g0089181 | 45.763 | 354 | 184 | 7 | 56 | 404 | 23 | 373 | 1.34e-97 | 296 |
| Msa0942210 | MtrunA17_Chr8g0373721 | 45.198 | 354 | 186 | 6 | 56 | 404 | 42 | 392 | 2.68e-97 | 296 |
| Msa0942210 | MtrunA17_Chr8g0373731 | 45.198 | 354 | 186 | 6 | 56 | 404 | 42 | 392 | 1.29e-91 | 281 |
| Msa0942210 | MtrunA17_Chr8g0373701 | 44.068 | 354 | 190 | 7 | 56 | 404 | 97 | 447 | 1.31e-89 | 278 |
| Msa0942210 | MtrunA17_Chr5g0403391 | 42.373 | 354 | 196 | 6 | 56 | 404 | 41 | 391 | 2.07e-84 | 263 |
| Msa0942210 | MtrunA17_Chr4g0031851 | 45.955 | 309 | 161 | 5 | 95 | 400 | 28 | 333 | 3.79e-84 | 261 |
| Msa0942210 | MtrunA17_Chr5g0414541 | 42.373 | 354 | 196 | 6 | 56 | 404 | 41 | 391 | 3.92e-84 | 262 |
| Msa0942210 | MtrunA17_Chr5g0403481 | 42.090 | 354 | 197 | 6 | 56 | 404 | 41 | 391 | 1.16e-83 | 261 |
| Msa0942210 | MtrunA17_Chr5g0403461 | 42.090 | 354 | 197 | 6 | 56 | 404 | 41 | 391 | 1.16e-83 | 261 |
| Msa0942210 | MtrunA17_Chr5g0403491 | 42.090 | 354 | 197 | 6 | 56 | 404 | 41 | 391 | 1.16e-83 | 261 |
| Msa0942210 | MtrunA17_Chr5g0403411 | 42.090 | 354 | 197 | 6 | 56 | 404 | 41 | 391 | 3.69e-81 | 254 |
| Msa0942210 | MtrunA17_Chr1g0183941 | 43.657 | 268 | 147 | 3 | 131 | 396 | 1 | 266 | 6.28e-69 | 219 |
| Msa0942210 | MtrunA17_Chr3g0113551 | 76.984 | 126 | 29 | 0 | 201 | 326 | 161 | 286 | 4.26e-68 | 218 |
| Msa0942210 | MtrunA17_Chr6g0481851 | 40.433 | 277 | 109 | 6 | 89 | 364 | 6 | 227 | 1.08e-52 | 176 |
| Msa0942210 | MtrunA17_Chr1g0212931 | 38.356 | 292 | 154 | 7 | 69 | 359 | 9 | 275 | 1.71e-49 | 170 |
| Msa0942210 | MtrunA17_Chr2g0288901 | 50.641 | 156 | 72 | 3 | 250 | 404 | 5 | 156 | 3.43e-48 | 161 |
| Msa0942210 | MtrunA17_Chr2g0288911 | 63.115 | 122 | 44 | 1 | 131 | 251 | 1 | 122 | 2.48e-43 | 147 |
| Msa0942210 | MtrunA17_Chr1g0182021 | 36.140 | 285 | 158 | 4 | 75 | 359 | 64 | 324 | 1.02e-39 | 145 |
| Msa0942210 | MtrunA17_Chr1g0183951 | 37.778 | 90 | 53 | 2 | 29 | 117 | 17 | 104 | 1.09e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0942210 | AT5G63180.1 | 73.020 | 404 | 106 | 2 | 4 | 404 | 29 | 432 | 0.0 | 616 |
| Msa0942210 | AT3G27400.2 | 72.167 | 406 | 106 | 3 | 4 | 404 | 13 | 416 | 0.0 | 613 |
| Msa0942210 | AT3G27400.1 | 72.167 | 406 | 106 | 3 | 4 | 404 | 9 | 412 | 0.0 | 613 |
| Msa0942210 | AT1G67750.1 | 73.779 | 389 | 100 | 1 | 16 | 404 | 22 | 408 | 0.0 | 607 |
| Msa0942210 | AT4G24780.2 | 72.388 | 402 | 109 | 2 | 4 | 404 | 8 | 408 | 0.0 | 593 |
| Msa0942210 | AT4G24780.1 | 72.388 | 402 | 109 | 2 | 4 | 404 | 8 | 408 | 0.0 | 593 |
| Msa0942210 | AT4G13710.1 | 66.412 | 393 | 128 | 1 | 16 | 404 | 78 | 470 | 0.0 | 588 |
| Msa0942210 | AT4G13710.2 | 66.412 | 393 | 128 | 1 | 16 | 404 | 59 | 451 | 0.0 | 585 |
| Msa0942210 | AT3G07010.1 | 68.069 | 404 | 122 | 1 | 8 | 404 | 13 | 416 | 0.0 | 572 |
| Msa0942210 | AT3G07010.2 | 70.822 | 377 | 110 | 0 | 28 | 404 | 7 | 383 | 0.0 | 567 |
| Msa0942210 | AT1G04680.1 | 67.358 | 386 | 122 | 1 | 23 | 404 | 44 | 429 | 0.0 | 554 |
| Msa0942210 | AT3G24670.1 | 68.325 | 382 | 121 | 0 | 23 | 404 | 59 | 440 | 0.0 | 550 |
| Msa0942210 | AT3G24230.1 | 65.026 | 386 | 130 | 2 | 23 | 404 | 67 | 451 | 0.0 | 545 |
| Msa0942210 | AT5G48900.1 | 64.663 | 416 | 133 | 2 | 3 | 404 | 2 | 417 | 0.0 | 536 |
| Msa0942210 | AT4G13210.2 | 68.407 | 383 | 120 | 1 | 23 | 404 | 56 | 438 | 0.0 | 527 |
| Msa0942210 | AT4G13210.1 | 68.407 | 383 | 120 | 1 | 23 | 404 | 19 | 401 | 0.0 | 525 |
| Msa0942210 | AT3G53190.1 | 61.259 | 413 | 143 | 3 | 1 | 396 | 2 | 414 | 3.95e-180 | 510 |
| Msa0942210 | AT5G04310.3 | 62.402 | 383 | 131 | 1 | 27 | 396 | 53 | 435 | 2.02e-171 | 488 |
| Msa0942210 | AT5G04310.1 | 62.402 | 383 | 131 | 1 | 27 | 396 | 53 | 435 | 2.82e-170 | 486 |
| Msa0942210 | AT5G04310.2 | 62.402 | 383 | 131 | 1 | 27 | 396 | 53 | 435 | 2.82e-170 | 486 |
| Msa0942210 | AT5G55720.1 | 55.831 | 403 | 165 | 4 | 4 | 404 | 1 | 392 | 2.83e-167 | 474 |
| Msa0942210 | AT3G54920.1 | 59.481 | 385 | 132 | 4 | 27 | 396 | 37 | 412 | 2.89e-167 | 478 |
| Msa0942210 | AT1G14420.1 | 54.211 | 380 | 166 | 2 | 33 | 404 | 80 | 459 | 4.24e-151 | 436 |
| Msa0942210 | AT1G11920.1 | 58.000 | 350 | 144 | 3 | 56 | 404 | 37 | 384 | 8.05e-148 | 424 |
| Msa0942210 | AT4G22090.1 | 57.429 | 350 | 145 | 3 | 56 | 404 | 47 | 393 | 7.57e-146 | 420 |
| Msa0942210 | AT3G01270.1 | 56.056 | 355 | 154 | 2 | 52 | 404 | 121 | 475 | 2.67e-145 | 421 |
| Msa0942210 | AT5G15110.1 | 55.211 | 355 | 157 | 2 | 52 | 404 | 118 | 472 | 3.17e-145 | 421 |
| Msa0942210 | AT4G22080.1 | 57.143 | 350 | 146 | 3 | 56 | 404 | 47 | 393 | 9.92e-144 | 414 |
| Msa0942210 | AT2G02720.1 | 53.521 | 355 | 159 | 1 | 56 | 404 | 101 | 455 | 3.66e-140 | 407 |
| Msa0942210 | AT1G30350.1 | 51.372 | 401 | 162 | 5 | 4 | 404 | 1 | 368 | 1.15e-139 | 403 |
| Msa0942210 | AT5G09280.1 | 47.984 | 248 | 127 | 2 | 89 | 335 | 6 | 252 | 5.71e-78 | 243 |
| Msa0942210 | AT3G55140.1 | 39.510 | 286 | 147 | 7 | 75 | 359 | 16 | 276 | 4.77e-50 | 172 |
| Msa0942210 | AT3G09540.1 | 36.111 | 288 | 158 | 5 | 73 | 359 | 61 | 323 | 3.23e-43 | 155 |
| Msa0942210 | AT3G55140.2 | 39.044 | 251 | 132 | 6 | 110 | 359 | 22 | 252 | 4.04e-42 | 150 |
| Msa0942210 | AT3G09540.3 | 35.764 | 288 | 155 | 6 | 73 | 359 | 61 | 319 | 3.36e-41 | 150 |
| Msa0942210 | AT3G09540.2 | 35.510 | 245 | 134 | 4 | 73 | 317 | 61 | 281 | 1.18e-35 | 132 |
Find 81 sgRNAs with CRISPR-Local
Find 149 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTTAATTCACAACATATT+TGG | 0.148343 | 6_3:-16739098 | Msa0942210:CDS |
| CACCAGAACATAGTGGATTT+TGG | 0.165374 | 6_3:-16739149 | Msa0942210:CDS |
| TGTCGGTTGTTCTCCCAATT+TGG | 0.183698 | 6_3:+16739525 | None:intergenic |
| GAAGAGGATGAAACAAAATT+TGG | 0.190078 | 6_3:+16740557 | None:intergenic |
| ATGCATACCTTGGCATTCTT+TGG | 0.240400 | 6_3:+16738881 | None:intergenic |
| ATCTACATTGTCACTGATTC+AGG | 0.262745 | 6_3:-16739441 | Msa0942210:CDS |
| TCAATTAAAACAAGAGCTTT+TGG | 0.287323 | 6_3:-16739322 | Msa0942210:CDS |
| TCATGACAAAGTTATGCTTT+TGG | 0.300199 | 6_3:-16738980 | Msa0942210:CDS |
| GCTGATTGTGCAATTGGATT+TGG | 0.305034 | 6_3:-16739495 | Msa0942210:CDS |
| GTCGGTTGTTCTCCCAATTT+GGG | 0.308400 | 6_3:+16739526 | None:intergenic |
| CAATTCAAGATGAACCATTA+TGG | 0.310393 | 6_3:-16739371 | Msa0942210:CDS |
| TCCTTGCTAAATCTATTATC+TGG | 0.317797 | 6_3:+16737987 | None:intergenic |
| TACCTTAATGTTCCTGGCTT+AGG | 0.318773 | 6_3:+16739399 | None:intergenic |
| GTTGGAGATGTGACCCAAAT+TGG | 0.323275 | 6_3:-16739539 | Msa0942210:CDS |
| GCACCATACCTTAATGTTCC+TGG | 0.337883 | 6_3:+16739393 | None:intergenic |
| TTCCCTTGGCTGTTGATTGT+AGG | 0.338196 | 6_3:+16738023 | None:intergenic |
| ACCTTAATGTTCCTGGCTTA+GGG | 0.354026 | 6_3:+16739400 | None:intergenic |
| ACATCTTGTACAACAAGTTC+AGG | 0.356255 | 6_3:+16740524 | None:intergenic |
| AGACTAGCTGATTGTGCAAT+TGG | 0.358822 | 6_3:-16739501 | Msa0942210:CDS |
| ACCCTAAGCCAGGAACATTA+AGG | 0.369613 | 6_3:-16739401 | Msa0942210:CDS |
| GTGAATATAATAGCTGGAAT+TGG | 0.370547 | 6_3:-16737935 | Msa0942210:CDS |
| CACAATCAGCTAGTCTTTGT+CGG | 0.381751 | 6_3:+16739508 | None:intergenic |
| GCAAGACCAGCCTCTCTTGT+TGG | 0.384223 | 6_3:-16737819 | Msa0942210:CDS |
| GAGGGTGATTTGTTCTTGAA+TGG | 0.394829 | 6_3:-16737906 | Msa0942210:CDS |
| CTCTAGGTCTAGGAGAAACT+TGG | 0.396252 | 6_3:-16739601 | Msa0942210:CDS |
| AAGAGGATGAAACAAAATTT+GGG | 0.396495 | 6_3:+16740558 | None:intergenic |
| GGCTATTTATCATGTGGAAC+TGG | 0.405816 | 6_3:-16739579 | Msa0942210:CDS |
| GTTCATGATTGTGTTCCAAC+GGG | 0.420422 | 6_3:-16739192 | Msa0942210:CDS |
| GGAGCATTCTTTAGACAAAC+TGG | 0.421504 | 6_3:-16737885 | Msa0942210:CDS |
| TCCCTTGGCTGTTGATTGTA+GGG | 0.426471 | 6_3:+16738024 | None:intergenic |
| AATTCATACAAGACAATTGA+TGG | 0.427271 | 6_3:-16739297 | Msa0942210:CDS |
| GATCATATTCAAAAGAGATA+TGG | 0.439191 | 6_3:-16739349 | Msa0942210:CDS |
| GCAATCCAATTGATGATTGT+TGG | 0.439325 | 6_3:-16739557 | Msa0942210:CDS |
| CATCTCCAACAATCATCAAT+TGG | 0.440002 | 6_3:+16739552 | None:intergenic |
| GGTTCCATCACTACTACATC+TGG | 0.450765 | 6_3:-16737798 | Msa0942210:CDS |
| GAACAATGATTACACACATT+GGG | 0.453403 | 6_3:-16738075 | Msa0942210:CDS |
| GTTAATAATGTTATAATACA+TGG | 0.461906 | 6_3:-16739222 | Msa0942210:CDS |
| CAGAAAGTGAATATAATAGC+TGG | 0.465031 | 6_3:-16737941 | Msa0942210:CDS |
| GTCCAAAATCCACTATGTTC+TGG | 0.466293 | 6_3:+16739147 | None:intergenic |
| GTGCAAGAAATCTGTTCCCT+TGG | 0.476596 | 6_3:+16738009 | None:intergenic |
| GCATCAATGTCTCTAGGTCT+AGG | 0.489694 | 6_3:-16739611 | Msa0942210:CDS |
| TTGGAGATGTGACCCAAATT+GGG | 0.502512 | 6_3:-16739538 | Msa0942210:CDS |
| CTAATATTTGTGTTCCCCGT+TGG | 0.503319 | 6_3:+16739177 | None:intergenic |
| GTAGGAGCATCAATGTCTCT+AGG | 0.514562 | 6_3:-16739617 | Msa0942210:intron |
| AGCTGGAATTGGAGATCAGA+GGG | 0.527960 | 6_3:-16737924 | Msa0942210:CDS |
| TCTTTGTCTAATTGTCGTGA+TGG | 0.533384 | 6_3:-16739063 | Msa0942210:CDS |
| CATTGGGAAATGTATGCAAT+TGG | 0.540092 | 6_3:-16738059 | Msa0942210:CDS |
| ATGGAACCAACAAGAGAGGC+TGG | 0.542918 | 6_3:+16737813 | None:intergenic |
| TGTTCATGATTGTGTTCCAA+CGG | 0.543560 | 6_3:-16739193 | Msa0942210:CDS |
| GGTCTTATTGATGTTATACA+TGG | 0.549548 | 6_3:-16739042 | Msa0942210:CDS |
| TGAACAATGATTACACACAT+TGG | 0.549687 | 6_3:-16738076 | Msa0942210:CDS |
| AGAGATTCACCAGAACATAG+TGG | 0.561715 | 6_3:-16739156 | Msa0942210:CDS |
| TGGATTTGGAAAAGATGCAA+TGG | 0.564287 | 6_3:-16739481 | Msa0942210:CDS |
| TGGTGTGCTTACTTGCAAGA+AGG | 0.564587 | 6_3:-16737778 | Msa0942210:CDS |
| TACAAGACAATTGATGGAAG+AGG | 0.566366 | 6_3:-16739291 | Msa0942210:CDS |
| AATTCCATCACCATCTGAAA+CGG | 0.566606 | 6_3:+16739125 | None:intergenic |
| ACCAGATAATAGATTTAGCA+AGG | 0.568377 | 6_3:-16737988 | Msa0942210:CDS |
| GGATTTGGAAAAGATGCAAT+GGG | 0.573388 | 6_3:-16739480 | Msa0942210:CDS |
| AACCCTACAATCAACAGCCA+AGG | 0.574532 | 6_3:-16738026 | Msa0942210:CDS |
| AACTTGGGCTATTTATCATG+TGG | 0.574554 | 6_3:-16739585 | Msa0942210:CDS |
| TGGACCGTTTCAGATGGTGA+TGG | 0.575388 | 6_3:-16739129 | Msa0942210:CDS |
| CACACCAGATGTAGTAGTGA+TGG | 0.576168 | 6_3:+16737794 | None:intergenic |
| CACACAAGACAAGGACATGC+AGG | 0.577203 | 6_3:-16738941 | Msa0942210:CDS |
| AAGCCAGGAACATTAAGGTA+TGG | 0.578760 | 6_3:-16739396 | Msa0942210:CDS |
| TTCAGGGTCTTGAACTGAAG+AGG | 0.579107 | 6_3:+16740541 | None:intergenic |
| GGTGTGCTTACTTGCAAGAA+GGG | 0.583647 | 6_3:-16737777 | Msa0942210:CDS |
| GCTAGTGTTCACATTGCAAA+TGG | 0.586396 | 6_3:-16739267 | Msa0942210:CDS |
| TGGGAAATGTATGCAATTGG+TGG | 0.593671 | 6_3:-16738056 | Msa0942210:CDS |
| CATCTTGTACAACAAGTTCA+GGG | 0.601679 | 6_3:+16740525 | None:intergenic |
| GATGCAGTAAACCCTAAGCC+AGG | 0.603794 | 6_3:-16739411 | Msa0942210:CDS |
| TCTAGGTCTAGGAGAAACTT+GGG | 0.631443 | 6_3:-16739600 | Msa0942210:CDS |
| ACCCTACAATCAACAGCCAA+GGG | 0.633679 | 6_3:-16738025 | Msa0942210:CDS |
| TGATTCTTACACACAAGACA+AGG | 0.643329 | 6_3:-16738950 | Msa0942210:CDS |
| AGTGTTCACATTGCAAATGG+TGG | 0.646316 | 6_3:-16739264 | Msa0942210:CDS |
| TAGCTGGAATTGGAGATCAG+AGG | 0.658304 | 6_3:-16737925 | Msa0942210:CDS |
| GGGAAATGTATGCAATTGGT+GGG | 0.659423 | 6_3:-16738055 | Msa0942210:CDS |
| AGTGATGGAACCAACAAGAG+AGG | 0.669990 | 6_3:+16737809 | None:intergenic |
| TTCATGATTGTGTTCCAACG+GGG | 0.684589 | 6_3:-16739191 | Msa0942210:CDS |
| TTGTTGTACAAGATGTACAA+AGG | 0.691137 | 6_3:-16740517 | Msa0942210:intron |
| GGCTTGTCCAAAGAATGCCA+AGG | 0.703891 | 6_3:-16738888 | Msa0942210:intron |
| TTTGGAAAAGATGCAATGGG+TGG | 0.760333 | 6_3:-16739477 | Msa0942210:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATTTTGTATTTTTTTATTT+TGG | + | chr6_3:16738243-16738262 | None:intergenic | 10.0% |
| !! | CATAATATATATAATTAACT+TGG | + | chr6_3:16738654-16738673 | None:intergenic | 10.0% |
| !! | CAATTACAAATAAAATTATT+TGG | + | chr6_3:16739760-16739779 | None:intergenic | 10.0% |
| !!! | AGACTTTTTTCTTTATAAAT+GGG | + | chr6_3:16738423-16738442 | None:intergenic | 15.0% |
| !!! | AAGACTTTTTTCTTTATAAA+TGG | + | chr6_3:16738424-16738443 | None:intergenic | 15.0% |
| !! | TTTATAAAGAAAAAAGTCTT+TGG | - | chr6_3:16738424-16738443 | Msa0942210:intron | 15.0% |
| !!! | ATTTTAATTTCTCTTTTTGT+AGG | - | chr6_3:16738731-16738750 | Msa0942210:intron | 15.0% |
| !! | GTTAATAATGTTATAATACA+TGG | - | chr6_3:16739144-16739163 | Msa0942210:CDS | 15.0% |
| !!! | AATATTTTTTTTTAGCATAG+TGG | - | chr6_3:16739541-16739560 | Msa0942210:CDS | 15.0% |
| !! | AAACCCAAATTAAAAAAAAT+TGG | - | chr6_3:16739628-16739647 | Msa0942210:intron | 15.0% |
| !! | AACCCAAATTAAAAAAAATT+GGG | - | chr6_3:16739629-16739648 | Msa0942210:intron | 15.0% |
| !!! | ACATTTTCTTTTATGTTTAT+GGG | + | chr6_3:16739927-16739946 | None:intergenic | 15.0% |
| !!! | TACATTTTCTTTTATGTTTA+TGG | + | chr6_3:16739928-16739947 | None:intergenic | 15.0% |
| !! | CTGAAAACAATAATTATTTA+TGG | + | chr6_3:16740244-16740263 | None:intergenic | 15.0% |
| !! | AAAGTTTGATTCATATGTAA+TGG | - | chr6_3:16737982-16738001 | Msa0942210:CDS | 20.0% |
| !! | TGTTTAATTCACAACATATT+TGG | - | chr6_3:16739268-16739287 | Msa0942210:CDS | 20.0% |
| !!! | ACTATTGCTTTTAATCATTT+TGG | - | chr6_3:16739450-16739469 | Msa0942210:CDS | 20.0% |
| !!! | TGCCCAATTTTTTTTAATTT+GGG | + | chr6_3:16739634-16739653 | None:intergenic | 20.0% |
| !!! | ATGCCCAATTTTTTTTAATT+TGG | + | chr6_3:16739635-16739654 | None:intergenic | 20.0% |
| !!! | TTTGTTTTTAAATGAGAAAC+TGG | - | chr6_3:16739667-16739686 | Msa0942210:intron | 20.0% |
| !! | ATGATACTACTAAGAAATTA+GGG | + | chr6_3:16740025-16740044 | None:intergenic | 20.0% |
| !!! | TTGAATTTTTTAAGGTAGTT+AGG | + | chr6_3:16740114-16740133 | None:intergenic | 20.0% |
| !!! | CCTACTTTTTGAATTTTTTA+AGG | + | chr6_3:16740122-16740141 | None:intergenic | 20.0% |
| !! | CCTTAAAAAATTCAAAAAGT+AGG | - | chr6_3:16740119-16740138 | Msa0942210:intron | 20.0% |
| !! | AAATTCAAAAAGTAGGATAA+AGG | - | chr6_3:16740126-16740145 | Msa0942210:intron | 20.0% |
| !! | TTGAACAAATAATAAGCAAT+AGG | + | chr6_3:16740178-16740197 | None:intergenic | 20.0% |
| ! | AAGAGGATGAAACAAAATTT+GGG | + | chr6_3:16737811-16737830 | None:intergenic | 25.0% |
| !! | CACATTAGATACAATGTTTT+TGG | + | chr6_3:16738008-16738027 | None:intergenic | 25.0% |
| ! | ATTACTCTATGATATCACTT+TGG | + | chr6_3:16738100-16738119 | None:intergenic | 25.0% |
| ! | ATTAATCTCAAAGCTCATAT+AGG | - | chr6_3:16738167-16738186 | Msa0942210:intron | 25.0% |
| !! | ATTTAATAGAAGTGCATTGT+TGG | - | chr6_3:16738342-16738361 | Msa0942210:intron | 25.0% |
| !! | CTTTTGAATATGATCCATAA+TGG | + | chr6_3:16739012-16739031 | None:intergenic | 25.0% |
| ! | GATCATATTCAAAAGAGATA+TGG | - | chr6_3:16739017-16739036 | Msa0942210:CDS | 25.0% |
| !! | TCAATTAAAACAAGAGCTTT+TGG | - | chr6_3:16739044-16739063 | Msa0942210:CDS | 25.0% |
| ! | AATTCATACAAGACAATTGA+TGG | - | chr6_3:16739069-16739088 | Msa0942210:CDS | 25.0% |
| !!! | CTTTTAATCATTTTGGTGAA+GGG | - | chr6_3:16739457-16739476 | Msa0942210:CDS | 25.0% |
| !! | TGTTAGCAAAAATGTGTTTT+TGG | - | chr6_3:16739582-16739601 | Msa0942210:CDS | 25.0% |
| !! | GTTAGCAAAAATGTGTTTTT+GGG | - | chr6_3:16739583-16739602 | Msa0942210:CDS | 25.0% |
| ! | AAGAAAATCTCTCTATAGTT+GGG | + | chr6_3:16739864-16739883 | None:intergenic | 25.0% |
| ! | AAAGAAAATCTCTCTATAGT+TGG | + | chr6_3:16739865-16739884 | None:intergenic | 25.0% |
| ! | CATGATACTACTAAGAAATT+AGG | + | chr6_3:16740026-16740045 | None:intergenic | 25.0% |
| ! | AATTTCTTAGTAGTATCATG+AGG | - | chr6_3:16740026-16740045 | Msa0942210:intron | 25.0% |
| !!! | TTACTTTGTTTTTGGTTCAA+TGG | + | chr6_3:16740054-16740073 | None:intergenic | 25.0% |
| !!! | TAAGGTCTTTACTTTGTTTT+TGG | + | chr6_3:16740062-16740081 | None:intergenic | 25.0% |
| ! | CACATTTCTTTGAATTTGTA+AGG | + | chr6_3:16740080-16740099 | None:intergenic | 25.0% |
| GAAGAGGATGAAACAAAATT+TGG | + | chr6_3:16737812-16737831 | None:intergenic | 30.0% | |
| TTGTTGTACAAGATGTACAA+AGG | - | chr6_3:16737849-16737868 | Msa0942210:CDS | 30.0% | |
| AGCTGAGAATAAACTAGAAT+CGG | - | chr6_3:16738271-16738290 | Msa0942210:intron | 30.0% | |
| ACAATGTGGTGAATTATCAT+CGG | + | chr6_3:16738392-16738411 | None:intergenic | 30.0% | |
| ! | AATGGGTTTATATGACAATG+TGG | + | chr6_3:16738406-16738425 | None:intergenic | 30.0% |
| GTTTAATTACTGTACATCGA+CGG | - | chr6_3:16738567-16738586 | Msa0942210:intron | 30.0% | |
| CAATTCAAGATGAACCATTA+TGG | - | chr6_3:16738995-16739014 | Msa0942210:CDS | 30.0% | |
| !! | GGTCTTATTGATGTTATACA+TGG | - | chr6_3:16739324-16739343 | Msa0942210:CDS | 30.0% |
| ! | TCATGACAAAGTTATGCTTT+TGG | - | chr6_3:16739386-16739405 | Msa0942210:CDS | 30.0% |
| ! | CATGACAAAGTTATGCTTTT+GGG | - | chr6_3:16739387-16739406 | Msa0942210:CDS | 30.0% |
| !! | GCTTTTAATCATTTTGGTGA+AGG | - | chr6_3:16739456-16739475 | Msa0942210:CDS | 30.0% |
| TGTCATATATATGCATACCT+TGG | + | chr6_3:16739498-16739517 | None:intergenic | 30.0% | |
| TAGAAAAATCTCTCTCCTAA+AGG | + | chr6_3:16739802-16739821 | None:intergenic | 30.0% | |
| ! | GCTATTATGAGAGATTTTGA+AGG | + | chr6_3:16739982-16740001 | None:intergenic | 30.0% |
| ! | ATTGTTTTCAGATGTAGACA+TGG | - | chr6_3:16740253-16740272 | Msa0942210:intron | 30.0% |
| ! | TAGACATGGATATTTTCATG+TGG | - | chr6_3:16740267-16740286 | Msa0942210:intron | 30.0% |
| TGAACAATGATTACACACAT+TGG | - | chr6_3:16740290-16740309 | Msa0942210:intron | 30.0% | |
| GAACAATGATTACACACATT+GGG | - | chr6_3:16740291-16740310 | Msa0942210:intron | 30.0% | |
| ACCAGATAATAGATTTAGCA+AGG | - | chr6_3:16740378-16740397 | Msa0942210:intron | 30.0% | |
| TCCTTGCTAAATCTATTATC+TGG | + | chr6_3:16740382-16740401 | None:intergenic | 30.0% | |
| CAGAAAGTGAATATAATAGC+TGG | - | chr6_3:16740425-16740444 | Msa0942210:intron | 30.0% | |
| GTGAATATAATAGCTGGAAT+TGG | - | chr6_3:16740431-16740450 | Msa0942210:intron | 30.0% | |
| CATCTTGTACAACAAGTTCA+GGG | + | chr6_3:16737844-16737863 | None:intergenic | 35.0% | |
| ACATCTTGTACAACAAGTTC+AGG | + | chr6_3:16737845-16737864 | None:intergenic | 35.0% | |
| AACTTGGGCTATTTATCATG+TGG | - | chr6_3:16738781-16738800 | Msa0942210:intron | 35.0% | |
| ! | GCAATCCAATTGATGATTGT+TGG | - | chr6_3:16738809-16738828 | Msa0942210:intron | 35.0% |
| CATCTCCAACAATCATCAAT+TGG | + | chr6_3:16738817-16738836 | None:intergenic | 35.0% | |
| TGGATTTGGAAAAGATGCAA+TGG | - | chr6_3:16738885-16738904 | Msa0942210:intron | 35.0% | |
| GGATTTGGAAAAGATGCAAT+GGG | - | chr6_3:16738886-16738905 | Msa0942210:intron | 35.0% | |
| !! | ATCTACATTGTCACTGATTC+AGG | - | chr6_3:16738925-16738944 | Msa0942210:CDS | 35.0% |
| TACAAGACAATTGATGGAAG+AGG | - | chr6_3:16739075-16739094 | Msa0942210:CDS | 35.0% | |
| TGTTCATGATTGTGTTCCAA+CGG | - | chr6_3:16739173-16739192 | Msa0942210:CDS | 35.0% | |
| AATTCCATCACCATCTGAAA+CGG | + | chr6_3:16739244-16739263 | None:intergenic | 35.0% | |
| TCTTTGTCTAATTGTCGTGA+TGG | - | chr6_3:16739303-16739322 | Msa0942210:CDS | 35.0% | |
| TGATTCTTACACACAAGACA+AGG | - | chr6_3:16739416-16739435 | Msa0942210:CDS | 35.0% | |
| GGGAGTAGAAATATTTGCTT+AGG | - | chr6_3:16739603-16739622 | Msa0942210:CDS | 35.0% | |
| TGTGTAAGATCAACTCCTTT+AGG | - | chr6_3:16739784-16739803 | Msa0942210:intron | 35.0% | |
| ! | GGAGAGAGATTTTTCTATCA+CGG | - | chr6_3:16739805-16739824 | Msa0942210:intron | 35.0% |
| TCTCTATAGTTGGGAATATG+GGG | + | chr6_3:16739855-16739874 | None:intergenic | 35.0% | |
| CTCTCTATAGTTGGGAATAT+GGG | + | chr6_3:16739856-16739875 | None:intergenic | 35.0% | |
| TCTCTCTATAGTTGGGAATA+TGG | + | chr6_3:16739857-16739876 | None:intergenic | 35.0% | |
| CATTGGGAAATGTATGCAAT+TGG | - | chr6_3:16740307-16740326 | Msa0942210:intron | 35.0% | |
| GGTGAATTATCATCGGATGT+CGG | + | chr6_3:16738385-16738404 | None:intergenic | 40.0% | |
| TCTAGGTCTAGGAGAAACTT+GGG | - | chr6_3:16738766-16738785 | Msa0942210:intron | 40.0% | |
| GGCTATTTATCATGTGGAAC+TGG | - | chr6_3:16738787-16738806 | Msa0942210:intron | 40.0% | |
| TTGGAGATGTGACCCAAATT+GGG | - | chr6_3:16738828-16738847 | Msa0942210:intron | 40.0% | |
| !! | CACAATCAGCTAGTCTTTGT+CGG | + | chr6_3:16738861-16738880 | None:intergenic | 40.0% |
| ! | AGACTAGCTGATTGTGCAAT+TGG | - | chr6_3:16738865-16738884 | Msa0942210:intron | 40.0% |
| GCTGATTGTGCAATTGGATT+TGG | - | chr6_3:16738871-16738890 | Msa0942210:intron | 40.0% | |
| TTTGGAAAAGATGCAATGGG+TGG | - | chr6_3:16738889-16738908 | Msa0942210:CDS | 40.0% | |
| GATGCAATGGGTGGTAAAAA+TGG | - | chr6_3:16738898-16738917 | Msa0942210:CDS | 40.0% | |
| ACCTTAATGTTCCTGGCTTA+GGG | + | chr6_3:16738969-16738988 | None:intergenic | 40.0% | |
| TACCTTAATGTTCCTGGCTT+AGG | + | chr6_3:16738970-16738989 | None:intergenic | 40.0% | |
| AAGCCAGGAACATTAAGGTA+TGG | - | chr6_3:16738970-16738989 | Msa0942210:CDS | 40.0% | |
| GCTAGTGTTCACATTGCAAA+TGG | - | chr6_3:16739099-16739118 | Msa0942210:CDS | 40.0% | |
| AGTGTTCACATTGCAAATGG+TGG | - | chr6_3:16739102-16739121 | Msa0942210:CDS | 40.0% | |
| GTTCATGATTGTGTTCCAAC+GGG | - | chr6_3:16739174-16739193 | Msa0942210:CDS | 40.0% | |
| TTCATGATTGTGTTCCAACG+GGG | - | chr6_3:16739175-16739194 | Msa0942210:CDS | 40.0% | |
| CTAATATTTGTGTTCCCCGT+TGG | + | chr6_3:16739192-16739211 | None:intergenic | 40.0% | |
| AGAGATTCACCAGAACATAG+TGG | - | chr6_3:16739210-16739229 | Msa0942210:CDS | 40.0% | |
| !!! | CACCAGAACATAGTGGATTT+TGG | - | chr6_3:16739217-16739236 | Msa0942210:CDS | 40.0% |
| GTCCAAAATCCACTATGTTC+TGG | + | chr6_3:16739222-16739241 | None:intergenic | 40.0% | |
| ATGCATACCTTGGCATTCTT+TGG | + | chr6_3:16739488-16739507 | None:intergenic | 40.0% | |
| GGGGTTATCTACATAGAGAT+GGG | + | chr6_3:16739893-16739912 | None:intergenic | 40.0% | |
| TGGGGTTATCTACATAGAGA+TGG | + | chr6_3:16739894-16739913 | None:intergenic | 40.0% | |
| TATGTAGATAACCCCACAAG+TGG | - | chr6_3:16739898-16739917 | Msa0942210:intron | 40.0% | |
| ATGTAGATAACCCCACAAGT+GGG | - | chr6_3:16739899-16739918 | Msa0942210:intron | 40.0% | |
| TGGGAAATGTATGCAATTGG+TGG | - | chr6_3:16740310-16740329 | Msa0942210:intron | 40.0% | |
| GGGAAATGTATGCAATTGGT+GGG | - | chr6_3:16740311-16740330 | Msa0942210:intron | 40.0% | |
| !! | GAGGGTGATTTGTTCTTGAA+TGG | - | chr6_3:16740460-16740479 | Msa0942210:intron | 40.0% |
| ! | GGAGCATTCTTTAGACAAAC+TGG | - | chr6_3:16740481-16740500 | Msa0942210:intron | 40.0% |
| TTCAGGGTCTTGAACTGAAG+AGG | + | chr6_3:16737828-16737847 | None:intergenic | 45.0% | |
| CATCGGATGTCGGTGTAAAA+TGG | + | chr6_3:16738375-16738394 | None:intergenic | 45.0% | |
| !! | GTAGGAGCATCAATGTCTCT+AGG | - | chr6_3:16738749-16738768 | Msa0942210:intron | 45.0% |
| GCATCAATGTCTCTAGGTCT+AGG | - | chr6_3:16738755-16738774 | Msa0942210:intron | 45.0% | |
| CTCTAGGTCTAGGAGAAACT+TGG | - | chr6_3:16738765-16738784 | Msa0942210:intron | 45.0% | |
| GTTGGAGATGTGACCCAAAT+TGG | - | chr6_3:16738827-16738846 | Msa0942210:intron | 45.0% | |
| GTCGGTTGTTCTCCCAATTT+GGG | + | chr6_3:16738843-16738862 | None:intergenic | 45.0% | |
| ! | TGTCGGTTGTTCTCCCAATT+TGG | + | chr6_3:16738844-16738863 | None:intergenic | 45.0% |
| ACCCTAAGCCAGGAACATTA+AGG | - | chr6_3:16738965-16738984 | Msa0942210:CDS | 45.0% | |
| GCACCATACCTTAATGTTCC+TGG | + | chr6_3:16738976-16738995 | None:intergenic | 45.0% | |
| ! | GGATTTTGGACCGTTTCAGA+TGG | - | chr6_3:16739231-16739250 | Msa0942210:CDS | 45.0% |
| TTTATGGGTTCCCACTTGTG+GGG | + | chr6_3:16739912-16739931 | None:intergenic | 45.0% | |
| GTTTATGGGTTCCCACTTGT+GGG | + | chr6_3:16739913-16739932 | None:intergenic | 45.0% | |
| TGTTTATGGGTTCCCACTTG+TGG | + | chr6_3:16739914-16739933 | None:intergenic | 45.0% | |
| AACCCTACAATCAACAGCCA+AGG | - | chr6_3:16740340-16740359 | Msa0942210:intron | 45.0% | |
| ACCCTACAATCAACAGCCAA+GGG | - | chr6_3:16740341-16740360 | Msa0942210:intron | 45.0% | |
| ! | TCCCTTGGCTGTTGATTGTA+GGG | + | chr6_3:16740345-16740364 | None:intergenic | 45.0% |
| ! | TTCCCTTGGCTGTTGATTGT+AGG | + | chr6_3:16740346-16740365 | None:intergenic | 45.0% |
| GTGCAAGAAATCTGTTCCCT+TGG | + | chr6_3:16740360-16740379 | None:intergenic | 45.0% | |
| TAGCTGGAATTGGAGATCAG+AGG | - | chr6_3:16740441-16740460 | Msa0942210:intron | 45.0% | |
| AGCTGGAATTGGAGATCAGA+GGG | - | chr6_3:16740442-16740461 | Msa0942210:intron | 45.0% | |
| ! | AGTGATGGAACCAACAAGAG+AGG | + | chr6_3:16740560-16740579 | None:intergenic | 45.0% |
| GGTTCCATCACTACTACATC+TGG | - | chr6_3:16740568-16740587 | Msa0942210:CDS | 45.0% | |
| CACACCAGATGTAGTAGTGA+TGG | + | chr6_3:16740575-16740594 | None:intergenic | 45.0% | |
| TGGTGTGCTTACTTGCAAGA+AGG | - | chr6_3:16740588-16740607 | Msa0942210:CDS | 45.0% | |
| GGTGTGCTTACTTGCAAGAA+GGG | - | chr6_3:16740589-16740608 | Msa0942210:CDS | 45.0% | |
| !!! | TTTTTTTTTTTTTTTTGTAA+AGG | - | chr6_3:16738477-16738496 | Msa0942210:intron | 5.0% |
| GATGCAGTAAACCCTAAGCC+AGG | - | chr6_3:16738955-16738974 | Msa0942210:CDS | 50.0% | |
| TGGACCGTTTCAGATGGTGA+TGG | - | chr6_3:16739237-16739256 | Msa0942210:CDS | 50.0% | |
| CACACAAGACAAGGACATGC+AGG | - | chr6_3:16739425-16739444 | Msa0942210:CDS | 50.0% | |
| GGCTTGTCCAAAGAATGCCA+AGG | - | chr6_3:16739478-16739497 | Msa0942210:CDS | 50.0% | |
| ATGGAACCAACAAGAGAGGC+TGG | + | chr6_3:16740556-16740575 | None:intergenic | 50.0% | |
| GCAAGACCAGCCTCTCTTGT+TGG | - | chr6_3:16740547-16740566 | Msa0942210:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 16737764 | 16740624 | 16737764 | ID=Msa0942210;Name=Msa0942210 |
| chr6_3 | mRNA | 16737764 | 16740624 | 16737764 | ID=Msa0942210-mRNA-1;Parent=Msa0942210;Name=Msa0942210-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|0|0|1|1|1|3|0|404 |
| chr6_3 | exon | 16740518 | 16740624 | 16740518 | ID=Msa0942210-mRNA-1:exon:5081;Parent=Msa0942210-mRNA-1 |
| chr6_3 | exon | 16738889 | 16739635 | 16738889 | ID=Msa0942210-mRNA-1:exon:5080;Parent=Msa0942210-mRNA-1 |
| chr6_3 | exon | 16737764 | 16738124 | 16737764 | ID=Msa0942210-mRNA-1:exon:5079;Parent=Msa0942210-mRNA-1 |
| chr6_3 | CDS | 16740518 | 16740624 | 16740518 | ID=Msa0942210-mRNA-1:cds;Parent=Msa0942210-mRNA-1 |
| chr6_3 | CDS | 16738889 | 16739635 | 16738889 | ID=Msa0942210-mRNA-1:cds;Parent=Msa0942210-mRNA-1 |
| chr6_3 | CDS | 16737764 | 16738124 | 16737764 | ID=Msa0942210-mRNA-1:cds;Parent=Msa0942210-mRNA-1 |
| Gene Sequence |
| Protein sequence |
