AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0722890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0722890 MtrunA17_Chr5g0413921 96.784 342 11 0 1 342 1 342 0.0 672
Msa0722890 MtrunA17_Chr5g0413931 74.855 346 81 2 1 341 1 345 0.0 521
Msa0722890 MtrunA17_Chr5g0413941 74.551 334 85 0 1 334 1 334 0.0 514
Msa0722890 MtrunA17_Chr5g0413951 72.923 325 88 0 5 329 5 329 2.16e-176 493
Msa0722890 MtrunA17_Chr7g0227921 44.828 319 167 3 12 326 16 329 1.85e-80 249
Msa0722890 MtrunA17_Chr5g0413961 41.088 331 184 3 19 342 26 352 5.88e-80 248
Msa0722890 MtrunA17_Chr4g0059311 45.312 320 172 3 10 327 14 332 3.38e-79 246
Msa0722890 MtrunA17_Chr3g0126101 41.590 327 179 4 12 333 11 330 7.25e-77 239
Msa0722890 MtrunA17_Chr1g0150151 41.433 321 183 4 12 328 16 335 3.55e-72 228
Msa0722890 MtrunA17_Chr6g0451211 41.562 320 177 4 12 326 17 331 4.52e-71 225
Msa0722890 MtrunA17_Chr6g0451251 44.771 306 161 3 24 326 29 329 8.60e-68 217
Msa0722890 MtrunA17_Chr6g0451201 39.941 338 187 4 12 340 17 347 9.51e-67 214
Msa0722890 MtrunA17_Chr6g0451261 41.118 304 163 2 11 306 15 310 1.13e-64 220
Msa0722890 MtrunA17_Chr6g0451171 40.127 314 179 3 16 326 21 328 6.08e-64 207
Msa0722890 MtrunA17_Chr3g0114731 36.308 325 202 4 12 335 23 343 5.41e-59 195
Msa0722890 MtrunA17_Chr7g0255561 34.891 321 203 4 12 327 19 338 3.88e-58 192
Msa0722890 MtrunA17_Chr7g0255581 34.891 321 203 4 12 327 19 338 3.88e-58 192
Msa0722890 MtrunA17_Chr8g0354121 34.472 322 202 5 12 326 18 337 8.23e-58 192
Msa0722890 MtrunA17_Chr3g0081421 33.724 341 214 6 3 336 13 348 2.92e-57 191
Msa0722890 MtrunA17_Chr3g0081511 34.545 330 204 6 4 326 14 338 2.41e-56 188
Msa0722890 MtrunA17_Chr3g0126091 32.945 343 217 5 12 342 16 357 6.27e-55 183
Msa0722890 MtrunA17_Chr3g0114691 34.194 310 190 5 31 332 44 347 1.14e-53 181
Msa0722890 MtrunA17_Chr3g0129601 32.827 329 210 4 8 332 36 357 7.81e-53 178
Msa0722890 MtrunA17_Chr1g0160171 34.663 326 201 4 12 331 21 340 9.09e-53 178
Msa0722890 MtrunA17_Chr1g0155761 30.172 348 223 3 4 332 18 364 1.25e-51 176
Msa0722890 MtrunA17_Chr6g0483401 33.333 333 212 4 16 341 19 348 1.31e-50 172
Msa0722890 MtrunA17_Chr1g0182291 33.846 325 199 7 13 328 18 335 2.18e-50 172
Msa0722890 MtrunA17_Chr1g0186641 31.889 323 201 7 22 333 24 338 1.19e-49 169
Msa0722890 MtrunA17_Chr7g0263101 33.951 324 199 6 12 327 18 334 1.76e-49 169
Msa0722890 MtrunA17_Chr1g0186611 31.269 323 203 7 22 333 27 341 6.34e-49 167
Msa0722890 MtrunA17_Chr3g0142131 30.409 342 223 4 4 331 17 357 1.09e-48 168
Msa0722890 MtrunA17_Chr1g0160181 31.737 334 219 6 12 339 23 353 4.77e-48 166
Msa0722890 MtrunA17_Chr1g0164831 32.399 321 201 7 23 333 19 333 1.35e-47 164
Msa0722890 MtrunA17_Chr6g0463971 31.454 337 225 4 2 334 11 345 1.58e-47 164
Msa0722890 MtrunA17_Chr1g0186571 31.928 332 205 7 12 333 18 338 6.53e-47 162
Msa0722890 MtrunA17_Chr8g0383731 34.839 310 192 5 25 326 23 330 1.91e-46 161
Msa0722890 MtrunA17_Chr3g0114711 31.044 364 206 9 13 336 20 378 2.20e-46 162
Msa0722890 MtrunA17_Chr2g0278561 31.965 341 224 5 2 335 10 349 1.27e-45 160
Msa0722890 MtrunA17_Chr2g0284261 31.250 304 205 3 23 325 24 324 1.71e-45 159
Msa0722890 MtrunA17_Chr5g0407991 32.047 337 211 5 11 331 13 347 2.99e-45 159
Msa0722890 MtrunA17_Chr8g0334761 32.317 328 216 5 12 336 15 339 4.88e-45 157
Msa0722890 MtrunA17_Chr1g0171301 31.290 310 208 5 27 333 36 343 5.55e-45 157
Msa0722890 MtrunA17_Chr5g0408031 32.641 337 205 7 12 329 15 348 8.08e-45 157
Msa0722890 MtrunA17_Chr5g0408001 32.432 333 205 6 12 327 15 344 2.73e-44 156
Msa0722890 MtrunA17_Chr1g0186561 31.429 315 200 6 27 333 36 342 3.97e-44 155
Msa0722890 MtrunA17_Chr1g0186601 29.936 314 205 6 27 333 36 341 4.91e-44 154
Msa0722890 MtrunA17_Chr2g0290611 32.110 327 218 3 2 325 7 332 1.30e-43 154
Msa0722890 MtrunA17_Chr5g0408041 31.519 349 214 8 5 331 6 351 1.59e-43 154
Msa0722890 MtrunA17_Chr1g0184221 27.246 345 231 4 12 341 21 360 2.73e-43 153
Msa0722890 MtrunA17_Chr4g0050941 32.184 348 205 6 12 342 17 350 3.10e-43 153
Msa0722890 MtrunA17_Chr6g0451191 37.864 206 125 1 121 326 1 203 1.48e-42 147
Msa0722890 MtrunA17_Chr3g0129591 31.611 329 213 5 15 335 13 337 1.84e-42 150
Msa0722890 MtrunA17_Chr2g0278541 29.429 333 223 4 3 325 8 338 3.79e-42 150
Msa0722890 MtrunA17_Chr4g0073241 31.454 337 217 5 3 332 4 333 4.90e-42 150
Msa0722890 MtrunA17_Chr2g0332001 31.909 351 224 7 3 342 34 380 1.22e-41 149
Msa0722890 MtrunA17_Chr2g0278551 30.982 326 223 1 2 325 7 332 1.27e-41 149
Msa0722890 MtrunA17_Chr7g0271561 28.182 330 227 5 12 332 12 340 2.39e-41 148
Msa0722890 MtrunA17_Chr4g0050891 30.462 325 217 5 3 320 9 331 3.38e-41 147
Msa0722890 MtrunA17_Chr3g0129571 29.254 335 220 6 12 335 12 340 3.92e-41 147
Msa0722890 MtrunA17_Chr3g0114701 31.003 329 222 4 12 336 14 341 3.42e-40 145
Msa0722890 MtrunA17_Chr5g0407971 29.972 357 219 8 12 343 16 366 3.66e-40 145
Msa0722890 MtrunA17_Chr4g0050881 30.909 330 210 6 12 331 14 335 1.82e-39 142
Msa0722890 MtrunA17_Chr4g0051021 28.963 328 225 4 3 325 10 334 2.41e-38 140
Msa0722890 MtrunA17_Chr8g0369841 29.762 336 214 6 10 327 12 343 2.46e-38 140
Msa0722890 MtrunA17_Chr1g0160111 27.987 318 202 6 33 333 33 340 6.85e-37 136
Msa0722890 MtrunA17_Chr5g0441761 28.086 324 226 4 6 328 13 330 8.56e-37 135
Msa0722890 MtrunA17_Chr2g0310441 29.730 296 205 3 32 325 35 329 1.96e-36 135
Msa0722890 MtrunA17_Chr8g0334771 30.395 329 226 3 12 338 15 342 3.42e-34 129
Msa0722890 MtrunA17_Chr1g0211801 32.189 233 153 3 23 253 27 256 2.45e-33 124
Msa0722890 MtrunA17_Chr5g0399281 26.220 328 236 3 2 325 3 328 9.24e-33 124
Msa0722890 MtrunA17_Chr4g0051001 30.392 306 206 4 3 303 27 330 2.76e-32 123
Msa0722890 MtrunA17_Chr8g0334751 28.228 333 236 3 12 342 16 347 8.01e-32 122
Msa0722890 MtrunA17_Chr3g0129581 28.024 339 224 5 15 341 13 343 5.56e-31 120
Msa0722890 MtrunA17_Chr5g0428881 25.220 341 236 5 11 344 22 350 2.65e-30 119
Msa0722890 MtrunA17_Chr4g0056081 25.982 331 240 4 2 327 9 339 2.54e-29 115
Msa0722890 MtrunA17_Chr1g0177221 29.918 244 167 4 12 253 23 264 4.12e-29 113
Msa0722890 MtrunA17_Chr3g0084091 28.936 235 166 1 92 325 15 249 2.17e-28 112
Msa0722890 MtrunA17_Chr4g0050931 25.680 331 198 5 3 325 8 298 2.38e-28 112
Msa0722890 MtrunA17_Chr5g0429001 27.586 261 170 3 92 344 36 285 5.37e-28 111
Msa0722890 MtrunA17_Chr5g0404811 26.636 214 155 1 120 331 6 219 2.25e-24 99.8
Msa0722890 MtrunA17_Chr8g0334711 32.642 193 126 3 12 202 15 205 4.10e-22 92.8
Msa0722890 MtrunA17_Chr6g0451181 49.485 97 49 0 24 120 29 125 4.57e-20 85.1
Msa0722890 MtrunA17_Chr6g0451241 56.923 65 28 0 262 326 16 80 1.73e-19 82.8
Msa0722890 MtrunA17_Chr2g0278021 30.047 213 140 3 121 325 1 212 3.33e-19 86.3
Msa0722890 MtrunA17_Chr8g0334721 35.185 108 70 0 218 325 2 109 1.03e-16 76.6
Msa0722890 MtrunA17_Chr5g0404801 35.537 121 76 2 1 120 1 120 4.88e-14 68.2
Msa0722890 MtrunA17_Chr1g0177231 29.630 81 57 0 253 333 28 108 7.10e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0722890 AT5G40240.1 43.491 338 186 2 10 342 22 359 3.11e-87 267
Msa0722890 AT5G40240.3 43.491 338 186 2 10 342 22 359 3.11e-87 267
Msa0722890 AT5G40240.2 43.491 338 186 2 10 342 36 373 9.20e-87 266
Msa0722890 AT5G40230.1 43.149 343 174 3 12 341 25 359 2.68e-84 259
Msa0722890 AT5G40210.1 45.231 325 159 5 4 327 10 316 2.02e-81 251
Msa0722890 AT3G28050.1 46.082 319 166 3 8 325 13 326 5.70e-80 248
Msa0722890 AT3G28080.1 40.113 354 186 6 4 341 11 354 1.10e-69 222
Msa0722890 AT4G15540.1 42.500 320 168 3 12 328 21 327 1.52e-69 221
Msa0722890 AT4G15540.2 42.500 320 168 3 12 328 21 327 1.52e-69 221
Msa0722890 AT3G28100.1 41.194 335 176 5 4 327 11 335 3.16e-69 220
Msa0722890 AT3G28070.1 42.651 347 186 7 4 341 14 356 1.57e-63 206
Msa0722890 AT3G28130.2 41.212 330 186 5 4 328 9 335 4.40e-63 204
Msa0722890 AT3G28080.2 38.701 354 183 7 4 341 11 346 2.70e-62 202
Msa0722890 AT2G39510.1 36.335 322 200 5 11 329 11 330 7.86e-57 189
Msa0722890 AT3G28080.5 38.951 267 138 5 91 341 8 265 2.66e-56 184
Msa0722890 AT3G28080.7 38.951 267 138 5 91 341 8 265 2.66e-56 184
Msa0722890 AT3G28080.8 38.951 267 138 5 91 341 8 265 2.66e-56 184
Msa0722890 AT3G28080.6 38.951 267 138 5 91 341 8 265 2.66e-56 184
Msa0722890 AT3G28070.2 42.692 260 137 6 91 341 8 264 2.72e-56 184
Msa0722890 AT5G07050.1 35.152 330 203 6 12 331 22 350 3.68e-56 188
Msa0722890 AT3G18200.2 32.344 337 216 5 1 332 28 357 7.80e-55 184
Msa0722890 AT3G28130.5 42.798 243 131 5 91 328 10 249 3.55e-53 176
Msa0722890 AT3G28130.1 42.798 243 131 5 91 328 10 249 3.55e-53 176
Msa0722890 AT3G28070.3 42.623 305 166 6 4 303 14 314 1.24e-51 174
Msa0722890 AT1G21890.1 30.931 333 217 4 12 333 15 345 1.63e-49 170
Msa0722890 AT4G08290.1 32.704 318 208 4 12 325 17 332 2.94e-49 169
Msa0722890 AT4G08300.1 31.098 328 217 4 12 333 15 339 4.03e-49 169
Msa0722890 AT1G09380.1 32.407 324 197 4 12 322 12 326 3.60e-48 166
Msa0722890 AT3G28080.3 36.232 345 143 7 12 341 18 300 1.41e-47 163
Msa0722890 AT3G30340.1 30.818 318 211 4 23 335 26 339 2.65e-47 164
Msa0722890 AT1G75500.1 30.499 341 226 5 2 333 14 352 4.91e-47 164
Msa0722890 AT1G75500.2 30.499 341 226 5 2 333 14 352 4.91e-47 164
Msa0722890 AT1G44800.1 29.630 324 224 4 12 333 15 336 2.12e-46 162
Msa0722890 AT1G70260.1 32.087 321 205 7 25 333 29 348 5.49e-46 160
Msa0722890 AT4G01440.1 28.662 314 222 2 23 335 25 337 1.49e-45 159
Msa0722890 AT2G37460.1 31.611 329 212 6 3 325 10 331 2.10e-45 159
Msa0722890 AT4G30420.2 31.611 329 216 6 3 325 4 329 6.08e-45 158
Msa0722890 AT4G30420.1 31.875 320 209 6 12 325 3 319 8.17e-45 157
Msa0722890 AT5G64700.1 33.016 315 202 5 27 333 27 340 8.28e-45 157
Msa0722890 AT2G40900.1 32.024 331 206 7 4 327 7 325 2.31e-44 157
Msa0722890 AT5G13670.1 30.723 332 221 6 1 326 1 329 4.19e-43 153
Msa0722890 AT3G28080.9 35.443 237 128 5 121 341 1 228 1.61e-42 147
Msa0722890 AT3G28070.5 39.130 230 128 6 121 341 1 227 3.47e-42 147
Msa0722890 AT3G56620.1 30.938 320 215 5 4 322 7 321 2.68e-41 148
Msa0722890 AT3G53210.1 28.909 339 224 5 4 333 6 336 5.64e-41 147
Msa0722890 AT4G28040.1 31.361 338 206 8 1 328 1 322 1.08e-40 146
Msa0722890 AT4G28040.5 31.361 338 206 8 1 328 1 322 1.08e-40 146
Msa0722890 AT4G28040.3 31.361 338 206 8 1 328 1 322 1.08e-40 146
Msa0722890 AT4G28040.4 31.361 338 206 8 1 328 1 322 1.08e-40 146
Msa0722890 AT4G28040.2 31.361 338 206 8 1 328 1 322 1.08e-40 146
Msa0722890 AT1G43650.1 31.546 317 212 4 12 327 11 323 1.19e-40 146
Msa0722890 AT1G21890.2 30.693 303 197 4 12 303 15 315 1.71e-40 145
Msa0722890 AT3G28100.3 38.258 264 143 4 4 256 11 265 2.47e-40 143
Msa0722890 AT3G28130.3 39.437 213 121 5 121 328 1 210 5.51e-40 141
Msa0722890 AT1G68170.2 31.642 335 205 5 4 325 10 333 5.60e-39 142
Msa0722890 AT1G68170.1 31.498 327 201 4 12 325 7 323 1.02e-38 141
Msa0722890 AT4G01440.3 28.772 285 201 2 23 306 25 308 3.16e-38 139
Msa0722890 AT1G60050.1 30.864 324 206 7 25 333 30 350 2.22e-37 138
Msa0722890 AT2G37450.2 31.132 318 189 5 12 325 13 304 3.81e-37 136
Msa0722890 AT3G28080.4 33.333 270 103 5 87 341 4 211 4.59e-37 133
Msa0722890 AT3G18200.1 32.618 233 147 3 104 332 1 227 1.03e-36 133
Msa0722890 AT5G45370.1 29.503 322 213 5 10 329 22 331 1.52e-36 135
Msa0722890 AT3G45870.1 29.268 328 220 2 4 320 8 334 6.09e-36 134
Msa0722890 AT5G47470.1 29.107 347 231 6 2 339 24 364 1.14e-35 133
Msa0722890 AT5G45370.2 28.869 336 221 6 10 329 22 355 1.39e-35 133
Msa0722890 AT5G45370.4 28.869 336 221 6 10 329 22 355 1.39e-35 133
Msa0722890 AT4G01430.1 29.154 319 212 8 23 332 24 337 1.63e-35 132
Msa0722890 AT5G47470.2 29.107 347 231 6 2 339 52 392 1.89e-35 133
Msa0722890 AT1G25270.1 30.312 320 219 3 12 328 7 325 3.52e-35 132
Msa0722890 AT5G47470.3 28.443 334 230 4 2 332 52 379 8.71e-35 131
Msa0722890 AT3G28130.4 39.286 252 146 4 4 250 9 258 8.75e-35 128
Msa0722890 AT4G01440.5 29.075 227 161 0 109 335 3 229 9.17e-35 128
Msa0722890 AT4G01440.2 29.075 227 161 0 109 335 3 229 9.17e-35 128
Msa0722890 AT4G08290.2 33.206 262 162 5 3 253 1 260 1.19e-34 128
Msa0722890 AT1G01070.1 28.754 313 213 6 23 329 31 339 3.44e-33 126
Msa0722890 AT4G19185.1 28.829 333 219 4 12 328 25 355 1.16e-32 125
Msa0722890 AT1G01070.2 28.808 302 199 7 34 329 1 292 1.79e-31 120
Msa0722890 AT3G30340.2 32.203 236 151 4 23 253 26 257 3.91e-31 119
Msa0722890 AT3G28100.2 40.952 210 105 3 4 203 11 211 4.32e-31 118
Msa0722890 AT3G45870.8 28.617 311 210 2 4 303 8 317 5.61e-31 119
Msa0722890 AT3G53210.2 28.800 250 163 3 92 333 37 279 1.14e-30 119
Msa0722890 AT1G11450.3 27.213 305 218 4 23 325 31 333 8.56e-30 117
Msa0722890 AT2G37450.3 32.489 237 151 3 93 325 1 232 1.44e-29 114
Msa0722890 AT4G01450.2 28.947 304 210 3 23 325 25 323 1.86e-29 116
Msa0722890 AT4G01430.2 30.000 290 189 8 23 303 24 308 3.07e-29 115
Msa0722890 AT4G01450.1 28.723 282 195 3 23 303 25 301 1.11e-28 114
Msa0722890 AT1G11450.2 27.599 279 198 4 51 327 8 284 4.64e-28 111
Msa0722890 AT1G11450.5 27.599 279 198 4 51 327 15 291 7.26e-28 111
Msa0722890 AT4G01440.4 29.185 233 163 2 22 253 24 255 9.87e-28 109
Msa0722890 AT3G28130.6 43.216 199 107 3 12 206 17 213 2.14e-27 107
Msa0722890 AT1G11460.1 27.184 309 214 8 23 325 31 334 2.74e-27 110
Msa0722890 AT5G64700.2 34.188 234 145 5 27 252 27 259 3.78e-27 108
Msa0722890 AT3G53210.3 29.323 266 170 6 4 259 6 263 4.64e-27 107
Msa0722890 AT4G19185.2 28.185 259 168 4 86 328 21 277 8.78e-27 108
Msa0722890 AT1G11450.1 28.099 242 172 2 85 325 1 241 4.19e-26 105
Msa0722890 AT4G01450.4 30.131 229 154 3 23 250 25 248 4.28e-26 105
Msa0722890 AT5G45370.6 27.953 254 167 4 91 329 1 253 4.79e-25 102
Msa0722890 AT5G45370.5 27.953 254 167 4 91 329 1 253 4.79e-25 102
Msa0722890 AT1G11450.4 26.502 283 204 4 23 303 31 311 1.18e-23 99.8
Msa0722890 AT1G43650.2 31.559 263 174 5 1 262 1 258 3.83e-23 97.4
Msa0722890 AT4G01450.3 31.551 187 122 3 23 208 25 206 1.39e-22 95.1
Msa0722890 AT3G45870.9 28.409 264 176 3 4 256 8 269 1.42e-21 93.2
Msa0722890 AT2G37450.1 29.858 211 139 3 119 325 60 265 4.91e-21 92.0
Msa0722890 AT3G45870.5 27.830 212 141 2 120 320 49 259 2.48e-20 90.5
Msa0722890 AT4G16620.1 24.925 333 236 5 6 332 10 334 8.25e-20 89.7
Msa0722890 AT3G28060.1 42.017 119 53 4 193 303 44 154 9.01e-19 82.4
Msa0722890 AT4G24980.1 27.347 245 129 9 86 328 44 241 5.58e-18 83.2
Msa0722890 AT4G01430.3 27.861 201 134 5 136 329 1 197 2.05e-17 80.5
Msa0722890 AT4G16620.4 27.686 242 162 4 96 332 4 237 3.25e-17 80.9
Msa0722890 AT4G16620.2 27.686 242 162 4 96 332 4 237 3.25e-17 80.9
Msa0722890 AT5G45370.3 26.906 223 147 4 122 329 74 295 3.85e-17 81.6
Msa0722890 AT3G45870.6 26.667 195 131 2 120 303 49 242 1.07e-15 76.3
Msa0722890 AT3G45870.7 26.667 195 131 2 120 303 49 242 1.07e-15 76.3
Msa0722890 AT3G45870.2 28.873 142 100 1 179 320 43 183 1.80e-13 69.7

Find 103 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTCGCCTAGGCACCTATTT+TGG 0.233543 5_1:-20408375 None:intergenic
CAGAGGGAAATTCAAAAGTT+TGG 0.240714 5_1:+20407251 Msa0722890:CDS
GGGAGTGAGAGCAAGGAAAT+TGG 0.245597 5_1:+20405572 Msa0722890:five_prime_UTR
ATATACAGACTTGGATTATA+AGG 0.260123 5_1:+20407149 Msa0722890:intron
CATAAAATTGAGGATGAAAT+TGG 0.268466 5_1:-20405797 None:intergenic
GTAATAAAATATGTACTATT+TGG 0.269620 5_1:+20408606 Msa0722890:three_prime_UTR
GAGTGTTACCACCCACTTCT+AGG 0.278870 5_1:-20405652 None:intergenic
GCCTTTCAAAATATAGATAT+TGG 0.282976 5_1:+20406806 Msa0722890:CDS
CCTCTGTTGTCAACCAAAAT+AGG 0.289207 5_1:+20408362 Msa0722890:CDS
CTTGTTACCACTAGCTCTTC+AGG 0.295870 5_1:-20407178 None:intergenic
AGCAGCCAAGCTAAAATAAT+TGG 0.302050 5_1:+20406716 Msa0722890:CDS
GGCACAGTGGTCTCCATTGC+AGG 0.326321 5_1:+20406737 Msa0722890:CDS
ATAGTTGTCTGTTCCTTAAC+GGG 0.347528 5_1:-20408492 None:intergenic
ACTTATTGTTGCAGCATGAT+TGG 0.348960 5_1:+20408254 Msa0722890:intron
TTAACTGTGACATTATATAA+AGG 0.353485 5_1:+20406764 Msa0722890:CDS
TCATACTTGCTTTAATTTCA+AGG 0.356799 5_1:+20406116 Msa0722890:CDS
AAGGAACAGACAACTATATA+AGG 0.361781 5_1:+20408498 Msa0722890:three_prime_UTR
GTTAAATCAGATTGGATAGT+TGG 0.366365 5_1:+20406851 Msa0722890:CDS
TTGAACATAGCTACATATAT+TGG 0.366626 5_1:-20408029 None:intergenic
TCCAATGGAGGGTCCTTAAC+GGG 0.376598 5_1:-20408452 None:intergenic
TATACAGACTTGGATTATAA+GGG 0.382696 5_1:+20407150 Msa0722890:intron
TTTGGATTCTCAGACCTGAT+AGG 0.386129 5_1:+20407269 Msa0722890:CDS
ACCTTTCAGTTAAATCAGAT+TGG 0.389374 5_1:+20406843 Msa0722890:CDS
TATAGTTGTCTGTTCCTTAA+CGG 0.393263 5_1:-20408493 None:intergenic
CATATATTGGTCCTTTCTTA+CGG 0.398364 5_1:-20408016 None:intergenic
AATATAGATATTGGAGGAAG+TGG 0.416383 5_1:+20406815 Msa0722890:CDS
AAAGGAAACATAGAGCAAAA+AGG 0.418237 5_1:-20405733 None:intergenic
CTCCAATGGAGGGTCCTTAA+CGG 0.418414 5_1:-20408453 None:intergenic
ATCAAATCAGCCACCAATGA+TGG 0.421771 5_1:+20405677 Msa0722890:CDS
CTCTCTCCTGGGTAACTAAC+GGG 0.421896 5_1:+20408526 Msa0722890:three_prime_UTR
TATAAGGACATTCTCTCTCC+TGG 0.428043 5_1:+20408514 Msa0722890:three_prime_UTR
ACAGCTACCAACTCCCTATC+AGG 0.429577 5_1:-20407283 None:intergenic
TGAAAGGCGTTACTAATCAA+TGG 0.433685 5_1:-20406791 None:intergenic
TTGGGAGACAGTCTTTATCT+TGG 0.441217 5_1:+20408095 Msa0722890:CDS
AGTGCAATGACCATTCCTAA+TGG 0.441266 5_1:-20408053 None:intergenic
GTGCAATGACCATTCCTAAT+GGG 0.462955 5_1:-20408052 None:intergenic
GGAGTTGTCCTTGTTAAGTA+AGG 0.466511 5_1:-20408430 None:intergenic
TAACTGTGACATTATATAAA+GGG 0.467351 5_1:+20406765 Msa0722890:CDS
GTAGCTATGTTCAACCCATT+AGG 0.469889 5_1:+20408038 Msa0722890:CDS
TTGAAGTGGGAGTGAGAGCA+AGG 0.476081 5_1:+20405565 Msa0722890:five_prime_UTR
GCAAGTATGAAGGTAAAAGC+AGG 0.494725 5_1:-20406103 None:intergenic
CCCCGTTAAGGACCCTCCAT+TGG 0.504947 5_1:+20408451 Msa0722890:three_prime_UTR
TTGGATTCTCAGACCTGATA+GGG 0.506332 5_1:+20407270 Msa0722890:CDS
TGCAGTTCAATTCCTAGAAG+TGG 0.506993 5_1:+20405640 Msa0722890:CDS
GTTAAGTAAGGACCCTCCAT+TGG 0.510063 5_1:-20408418 None:intergenic
ACAAGGACAACTCCCCGTTA+AGG 0.521691 5_1:+20408439 Msa0722890:three_prime_UTR
GGAGCTGCTATAATAGCCAT+TGG 0.524019 5_1:+20408275 Msa0722890:CDS
AGTGAGAGCAAGGAAATTGG+AGG 0.525885 5_1:+20405575 Msa0722890:five_prime_UTR
TGAAATTAAAGCAAGTATGA+AGG 0.529601 5_1:-20406113 None:intergenic
TTAGGAATGGTCATTGCACT+TGG 0.530569 5_1:+20408056 Msa0722890:CDS
TATTACTTCTTTATGCAAGT+GGG 0.533976 5_1:-20408589 None:intergenic
TTATTACTTCTTTATGCAAG+TGG 0.541619 5_1:-20408590 None:intergenic
CGTTAAGGACCCTCCATTGG+AGG 0.542327 5_1:+20408454 Msa0722890:three_prime_UTR
GTAATAAAATGTGTACTACT+TGG 0.542705 5_1:+20408647 Msa0722890:three_prime_UTR
GCACAGTGGTCTCCATTGCA+GGG 0.543263 5_1:+20406738 Msa0722890:CDS
AAAATTGAGGATGAAATTGG+AGG 0.543678 5_1:-20405794 None:intergenic
GAGACAGTCTTTATCTTGGA+AGG 0.547932 5_1:+20408099 Msa0722890:CDS
CACAGTTAATGCCCCTGCAA+TGG 0.548584 5_1:-20406750 None:intergenic
ACATGGGCATTCCGTAAGAA+AGG 0.551083 5_1:+20408005 Msa0722890:CDS
CACAGTGGTCTCCATTGCAG+GGG 0.557629 5_1:+20406739 Msa0722890:CDS
TTATTACTTCTTTATGCAAG+TGG 0.560226 5_1:-20408631 None:intergenic
AATGGTCATTGCACTTGGCA+TGG 0.561423 5_1:+20408061 Msa0722890:CDS
GGACAGCACAATTATTGAAG+TGG 0.561827 5_1:+20405551 Msa0722890:five_prime_UTR
TTTCAAAATATAGATATTGG+AGG 0.562558 5_1:+20406809 Msa0722890:CDS
TATTACTTCTTTATGCAAGT+GGG 0.564425 5_1:-20408630 None:intergenic
AATACTCATTCCATCATTGG+TGG 0.565236 5_1:-20405687 None:intergenic
ATAAGGACATTCTCTCTCCT+GGG 0.568019 5_1:+20408515 Msa0722890:three_prime_UTR
TCTCTCTCCTGGGTAACTAA+CGG 0.570460 5_1:+20408525 Msa0722890:three_prime_UTR
TGAAGGTAAAAGCAGGGAGA+AGG 0.572430 5_1:-20406096 None:intergenic
TCCAATCTGATTTAACTGAA+AGG 0.573414 5_1:-20406844 None:intergenic
GCAGTTCAATTCCTAGAAGT+GGG 0.574665 5_1:+20405641 Msa0722890:CDS
AGCATAGCAGATGCTAGTGT+GGG 0.575963 5_1:-20406070 None:intergenic
AAGCATAGCAGATGCTAGTG+TGG 0.579129 5_1:-20406071 None:intergenic
ATTGAGGATGAAATTGGAGG+AGG 0.581877 5_1:-20405791 None:intergenic
ATGGTCATTGCACTTGGCAT+GGG 0.582373 5_1:+20408062 Msa0722890:CDS
GGACAATATACAAAACAGAA+AGG 0.582580 5_1:-20406904 None:intergenic
GTCAACCAAAATAGGTGCCT+AGG 0.585021 5_1:+20408370 Msa0722890:CDS
TTACAAATTCAAAGTCGCCT+AGG 0.585263 5_1:-20408387 None:intergenic
CAAGTATGAAGGTAAAAGCA+GGG 0.587024 5_1:-20406102 None:intergenic
TCTCAGACCTGATAGGGAGT+TGG 0.589811 5_1:+20407276 Msa0722890:CDS
TATGTTCAACCCATTAGGAA+TGG 0.601626 5_1:+20408043 Msa0722890:CDS
TGAGGAGTGTTGTATACACA+TGG 0.602238 5_1:+20407988 Msa0722890:CDS
CATAACTGCATAAAATCCAA+TGG 0.603922 5_1:-20408291 None:intergenic
AGCAAGTGTAACAGCAACAA+TGG 0.605117 5_1:+20405616 Msa0722890:CDS
TATGTGGGCACAAGCTCAAG+AGG 0.606878 5_1:+20408310 Msa0722890:CDS
GGATTATCCTGAAGAGCTAG+TGG 0.609543 5_1:+20407171 Msa0722890:CDS
TGTAAGAGCATCTCCAATGG+AGG 0.612515 5_1:+20408405 Msa0722890:three_prime_UTR
ATTTGTAAGAGCATCTCCAA+TGG 0.614542 5_1:+20408402 Msa0722890:three_prime_UTR
GACAGCACAATTATTGAAGT+GGG 0.617063 5_1:+20405552 Msa0722890:five_prime_UTR
TCATTTATAGGCAATTTGTG+TGG 0.623525 5_1:+20407959 Msa0722890:intron
GTGGGACCCGTTAGTTACCC+AGG 0.625794 5_1:-20408532 None:intergenic
TGGAGGGTCCTTACTTAACA+AGG 0.631921 5_1:+20408422 Msa0722890:three_prime_UTR
ATTGTAGCTCTCATTGCAGA+GGG 0.632720 5_1:+20407235 Msa0722890:CDS
AACAAGCATTTGTTGCAGCA+TGG 0.633651 5_1:+20407195 Msa0722890:CDS
CCAATGGAGGGTCCTTAACG+GGG 0.647949 5_1:-20408451 None:intergenic
GTAAGAGCATCTCCAATGGA+GGG 0.652205 5_1:+20408406 Msa0722890:three_prime_UTR
AATTGTAGCTCTCATTGCAG+AGG 0.661606 5_1:+20407234 Msa0722890:CDS
CAATTTGTGTGGTATCAATG+AGG 0.677875 5_1:+20407970 Msa0722890:CDS
AGCTAAAATAATTGGCACAG+TGG 0.680195 5_1:+20406724 Msa0722890:CDS
AAGCATTTGTTGCAGCATGG+TGG 0.684295 5_1:+20407198 Msa0722890:CDS
TAGTTGTCTGTTCCTTAACG+GGG 0.684918 5_1:-20408491 None:intergenic
GTTCAATTCCTAGAAGTGGG+TGG 0.702602 5_1:+20405644 Msa0722890:CDS
GAGGAGTGTTGTATACACAT+GGG 0.704060 5_1:+20407989 Msa0722890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAACAGAAAAATAATA+TGG + chr5_1:20406641-20406660 Msa0722890:intron 10.0%
!! TCAAAAAAAAAAAAACAAAT+TGG - chr5_1:20408153-20408172 None:intergenic 10.0%
!!! ATTTGTTTTTTTTTTTTTGA+AGG + chr5_1:20408153-20408172 Msa0722890:intron 10.0%
!!! TTTATTATTTTTTTAAACGT+GGG - chr5_1:20408553-20408572 None:intergenic 10.0%
!!! TTTTATTATTTTTTTAAACG+TGG - chr5_1:20408554-20408573 None:intergenic 10.0%
!! AAAAATTCTACATAAAATTG+AGG - chr5_1:20405810-20405829 None:intergenic 15.0%
!!! TCAAAGTAAATATTTTTGTA+TGG + chr5_1:20405919-20405938 Msa0722890:intron 15.0%
!! TATGAATTTAGTTGTATAAA+AGG + chr5_1:20405943-20405962 Msa0722890:intron 15.0%
!!! GATATATTTCAATCATTTTT+TGG + chr5_1:20406575-20406594 Msa0722890:intron 15.0%
!!! AGAGATTGATATTTTTAAAA+CGG + chr5_1:20407065-20407084 Msa0722890:intron 15.0%
!!! TTAGATTATTTCTAACAAAT+TGG - chr5_1:20408187-20408206 None:intergenic 15.0%
!! GTAATAAAATATGTACTATT+TGG + chr5_1:20408606-20408625 Msa0722890:three_prime_UTR 15.0%
!!! TCTTTTTCTATGGTAAAAAA+AGG - chr5_1:20405768-20405787 None:intergenic 20.0%
!! AGGAAATTTATGTGAAAATT+TGG + chr5_1:20405963-20405982 Msa0722890:intron 20.0%
!! GTAAAAAATTTAATGTCACT+CGG + chr5_1:20406262-20406281 Msa0722890:intron 20.0%
!!! ATTTTTCTGTTTTTTTTGCA+AGG - chr5_1:20406637-20406656 None:intergenic 20.0%
!! TTAACTGTGACATTATATAA+AGG + chr5_1:20406764-20406783 Msa0722890:CDS 20.0%
!! TAACTGTGACATTATATAAA+GGG + chr5_1:20406765-20406784 Msa0722890:CDS 20.0%
!!! TCCAATATCTATATTTTGAA+AGG - chr5_1:20406810-20406829 None:intergenic 20.0%
!! TTTCAAAATATAGATATTGG+AGG + chr5_1:20406809-20406828 Msa0722890:CDS 20.0%
!! GATATAACTATATGTAAACA+TGG + chr5_1:20406993-20407012 Msa0722890:intron 20.0%
!!! ATGTCATTAATGGTTTTATA+CGG + chr5_1:20407544-20407563 Msa0722890:intron 20.0%
!!! CATATTTTACATCAATAACA+TGG - chr5_1:20407731-20407750 None:intergenic 20.0%
!! GAATTATAATTTAACCAATG+TGG + chr5_1:20407920-20407939 Msa0722890:intron 20.0%
!!! TATTACTCATTTTTACAAGT+GGG - chr5_1:20408674-20408693 None:intergenic 20.0%
! AAAAATACTCATTCCATCAT+TGG - chr5_1:20405693-20405712 None:intergenic 25.0%
! CATAAAATTGAGGATGAAAT+TGG - chr5_1:20405800-20405819 None:intergenic 25.0%
!! ATAAAATTTGTTCATACCTG+AGG - chr5_1:20405844-20405863 None:intergenic 25.0%
! TGAAATTAAAGCAAGTATGA+AGG - chr5_1:20406116-20406135 None:intergenic 25.0%
!! TCATACTTGCTTTAATTTCA+AGG + chr5_1:20406116-20406135 Msa0722890:CDS 25.0%
!!! TTTTATACCCCTTTTTGAAA+AGG + chr5_1:20406456-20406475 Msa0722890:intron 25.0%
!! AAAGATTCCTTTTCAAAAAG+GGG - chr5_1:20406466-20406485 None:intergenic 25.0%
!! CAAAGATTCCTTTTCAAAAA+GGG - chr5_1:20406467-20406486 None:intergenic 25.0%
!! ACAAAGATTCCTTTTCAAAA+AGG - chr5_1:20406468-20406487 None:intergenic 25.0%
!! TTTTTGCAAGGATTAAAGAA+GGG - chr5_1:20406625-20406644 None:intergenic 25.0%
!!! TTTTTTGCAAGGATTAAAGA+AGG - chr5_1:20406626-20406645 None:intergenic 25.0%
! GCCTTTCAAAATATAGATAT+TGG + chr5_1:20406806-20406825 Msa0722890:CDS 25.0%
! TTCGAAAAGATTTATTTACC+TGG - chr5_1:20406928-20406947 None:intergenic 25.0%
!! AAGATTTTAAATCGTAGTTG+CGG + chr5_1:20406960-20406979 Msa0722890:intron 25.0%
! ATATACAGACTTGGATTATA+AGG + chr5_1:20407149-20407168 Msa0722890:intron 25.0%
! TATACAGACTTGGATTATAA+GGG + chr5_1:20407150-20407169 Msa0722890:intron 25.0%
! AATGACATTGCATAGAAAAA+GGG - chr5_1:20407532-20407551 None:intergenic 25.0%
! TAATGACATTGCATAGAAAA+AGG - chr5_1:20407533-20407552 None:intergenic 25.0%
! TTTCTATGCAATGTCATTAA+TGG + chr5_1:20407534-20407553 Msa0722890:intron 25.0%
! ACATGATAAGATATGATTAG+AGG - chr5_1:20407571-20407590 None:intergenic 25.0%
! ATTCTCTAACAAAGATTAAC+AGG - chr5_1:20407756-20407775 None:intergenic 25.0%
!!! ATGCATCACAACTTTAAAAT+CGG + chr5_1:20407890-20407909 Msa0722890:intron 25.0%
!! TTTGTGGTTGTTTCATTTAT+AGG + chr5_1:20407947-20407966 Msa0722890:intron 25.0%
! TTGAACATAGCTACATATAT+TGG - chr5_1:20408032-20408051 None:intergenic 25.0%
! TATTACTTCTTTATGCAAGT+GGG - chr5_1:20408592-20408611 None:intergenic 25.0%
! TTATTACTTCTTTATGCAAG+TGG - chr5_1:20408593-20408612 None:intergenic 25.0%
! TATTACTTCTTTATGCAAGT+GGG - chr5_1:20408592-20408611 None:intergenic 25.0%
! TTATTACTTCTTTATGCAAG+TGG - chr5_1:20408593-20408612 None:intergenic 25.0%
! GTAATAAAATGTGTACTACT+TGG + chr5_1:20408647-20408666 Msa0722890:three_prime_UTR 25.0%
!!! CTATTACTCATTTTTACAAG+TGG - chr5_1:20408675-20408694 None:intergenic 25.0%
AAAGGAAACATAGAGCAAAA+AGG - chr5_1:20405736-20405755 None:intergenic 30.0%
! AAAAAAAGGTGCATGGTAAA+AGG - chr5_1:20405754-20405773 None:intergenic 30.0%
AAAATTGAGGATGAAATTGG+AGG - chr5_1:20405797-20405816 None:intergenic 30.0%
!! GTGCAGATTTTAACGAATAT+TGG + chr5_1:20406031-20406050 Msa0722890:CDS 30.0%
GAAAATTGTGTTCAATGTGA+AGG + chr5_1:20406293-20406312 Msa0722890:intron 30.0%
! GCAAAATCATCAACTTTTCA+GGG - chr5_1:20406436-20406455 None:intergenic 30.0%
! AGCAAAATCATCAACTTTTC+AGG - chr5_1:20406437-20406456 None:intergenic 30.0%
! TTTTCCAGTAGAACTACAAA+TGG - chr5_1:20406533-20406552 None:intergenic 30.0%
AATATAGATATTGGAGGAAG+TGG + chr5_1:20406815-20406834 Msa0722890:CDS 30.0%
ACCTTTCAGTTAAATCAGAT+TGG + chr5_1:20406843-20406862 Msa0722890:CDS 30.0%
TCCAATCTGATTTAACTGAA+AGG - chr5_1:20406847-20406866 None:intergenic 30.0%
GTTAAATCAGATTGGATAGT+TGG + chr5_1:20406851-20406870 Msa0722890:CDS 30.0%
GGACAATATACAAAACAGAA+AGG - chr5_1:20406907-20406926 None:intergenic 30.0%
! TTCTGTTTTGTATATTGTCC+AGG + chr5_1:20406907-20406926 Msa0722890:CDS 30.0%
TAAATTCCCAATTTAGTGCA+AGG - chr5_1:20407106-20407125 None:intergenic 30.0%
TCTGCATATATATACAGACT+TGG + chr5_1:20407140-20407159 Msa0722890:intron 30.0%
TTGTATGCAATGTCATTGAA+AGG + chr5_1:20407379-20407398 Msa0722890:intron 30.0%
ATTGCGTACAATACACAAAA+TGG + chr5_1:20407456-20407475 Msa0722890:intron 30.0%
CTTTATTCACATATAGGTCT+AGG - chr5_1:20407846-20407865 None:intergenic 30.0%
CTAGACCTATATGTGAATAA+AGG + chr5_1:20407844-20407863 Msa0722890:intron 30.0%
TGATGCCTTTATTCACATAT+AGG - chr5_1:20407852-20407871 None:intergenic 30.0%
TCATTTATAGGCAATTTGTG+TGG + chr5_1:20407959-20407978 Msa0722890:intron 30.0%
CATATATTGGTCCTTTCTTA+CGG - chr5_1:20408019-20408038 None:intergenic 30.0%
CATAACTGCATAAAATCCAA+TGG - chr5_1:20408294-20408313 None:intergenic 30.0%
! TTGGATTTTATGCAGTTATG+TGG + chr5_1:20408294-20408313 Msa0722890:CDS 30.0%
! TGGATTTTATGCAGTTATGT+GGG + chr5_1:20408295-20408314 Msa0722890:CDS 30.0%
TATAGTTGTCTGTTCCTTAA+CGG - chr5_1:20408496-20408515 None:intergenic 30.0%
AAGGAACAGACAACTATATA+AGG + chr5_1:20408498-20408517 Msa0722890:three_prime_UTR 30.0%
GACAGCACAATTATTGAAGT+GGG + chr5_1:20405552-20405571 Msa0722890:five_prime_UTR 35.0%
CAAGGAAATTGGAGGAAAAA+TGG + chr5_1:20405583-20405602 Msa0722890:exon 35.0%
AATACTCATTCCATCATTGG+TGG - chr5_1:20405690-20405709 None:intergenic 35.0%
! CTATGGTAAAAAAAGGTGCA+TGG - chr5_1:20405761-20405780 None:intergenic 35.0%
! TTTTTCTTCTTAGTTGCCTC+AGG + chr5_1:20405825-20405844 Msa0722890:CDS 35.0%
! ATTTTCTCCTTCAAATCCTC+CGG + chr5_1:20405885-20405904 Msa0722890:intron 35.0%
CAAGTATGAAGGTAAAAGCA+GGG - chr5_1:20406105-20406124 None:intergenic 35.0%
TCCACTAACATCTATGACTT+GGG - chr5_1:20406207-20406226 None:intergenic 35.0%
TTCCACTAACATCTATGACT+TGG - chr5_1:20406208-20406227 None:intergenic 35.0%
AAGTCATAGATGTTAGTGGA+AGG + chr5_1:20406207-20406226 Msa0722890:intron 35.0%
ACCAAATACAATTGCCACAT+CGG - chr5_1:20406244-20406263 None:intergenic 35.0%
! TATGCCATTTGTAGTTCTAC+TGG + chr5_1:20406526-20406545 Msa0722890:intron 35.0%
!!! TTATTTTAGCTTGGCTGCTA+TGG - chr5_1:20406715-20406734 None:intergenic 35.0%
AGCAGCCAAGCTAAAATAAT+TGG + chr5_1:20406716-20406735 Msa0722890:CDS 35.0%
!!! CTGTGCCAATTATTTTAGCT+TGG - chr5_1:20406724-20406743 None:intergenic 35.0%
AGCTAAAATAATTGGCACAG+TGG + chr5_1:20406724-20406743 Msa0722890:CDS 35.0%
!! TGAAAGGCGTTACTAATCAA+TGG - chr5_1:20406794-20406813 None:intergenic 35.0%
! TTTTAAATCGTAGTTGCGGT+TGG + chr5_1:20406964-20406983 Msa0722890:intron 35.0%
TTTAAATCGTAGTTGCGGTT+GGG + chr5_1:20406965-20406984 Msa0722890:intron 35.0%
CACTTACCTTGCACTAAATT+GGG + chr5_1:20407097-20407116 Msa0722890:intron 35.0%
CAGAGGGAAATTCAAAAGTT+TGG + chr5_1:20407251-20407270 Msa0722890:CDS 35.0%
CAATGTCATTGAAAGGTCAA+TGG + chr5_1:20407386-20407405 Msa0722890:intron 35.0%
CGTACAATACACAAAATGGT+AGG + chr5_1:20407460-20407479 Msa0722890:intron 35.0%
! TTGTAAAACCAGCTTTTAGC+AGG + chr5_1:20407776-20407795 Msa0722890:intron 35.0%
AGGTCTAGGTTCAAATATTG+TGG - chr5_1:20407832-20407851 None:intergenic 35.0%
CAATTTGTGTGGTATCAATG+AGG + chr5_1:20407970-20407989 Msa0722890:CDS 35.0%
TATGTTCAACCCATTAGGAA+TGG + chr5_1:20408043-20408062 Msa0722890:CDS 35.0%
! TGGCATGGGTGTAATATTTT+TGG + chr5_1:20408076-20408095 Msa0722890:CDS 35.0%
! GGCATGGGTGTAATATTTTT+GGG + chr5_1:20408077-20408096 Msa0722890:CDS 35.0%
ACTTATTGTTGCAGCATGAT+TGG + chr5_1:20408254-20408273 Msa0722890:intron 35.0%
!!! TATTTTGGTTGACAACAGAG+GGG - chr5_1:20408363-20408382 None:intergenic 35.0%
!!! CTATTTTGGTTGACAACAGA+GGG - chr5_1:20408364-20408383 None:intergenic 35.0%
TTACAAATTCAAAGTCGCCT+AGG - chr5_1:20408390-20408409 None:intergenic 35.0%
!! ATTTGTAAGAGCATCTCCAA+TGG + chr5_1:20408402-20408421 Msa0722890:three_prime_UTR 35.0%
! ATTTTTCTTCCTCCAATGGA+GGG - chr5_1:20408466-20408485 None:intergenic 35.0%
! AATTTTTCTTCCTCCAATGG+AGG - chr5_1:20408467-20408486 None:intergenic 35.0%
! CTCAATTTTTCTTCCTCCAA+TGG - chr5_1:20408470-20408489 None:intergenic 35.0%
ATAGTTGTCTGTTCCTTAAC+GGG - chr5_1:20408495-20408514 None:intergenic 35.0%
GGACAGCACAATTATTGAAG+TGG + chr5_1:20405551-20405570 Msa0722890:five_prime_UTR 40.0%
AGCAAGTGTAACAGCAACAA+TGG + chr5_1:20405616-20405635 Msa0722890:CDS 40.0%
TGCAGTTCAATTCCTAGAAG+TGG + chr5_1:20405640-20405659 Msa0722890:CDS 40.0%
GCAGTTCAATTCCTAGAAGT+GGG + chr5_1:20405641-20405660 Msa0722890:CDS 40.0%
ATCAAATCAGCCACCAATGA+TGG + chr5_1:20405677-20405696 Msa0722890:CDS 40.0%
! GAGGAGGAGATCTTTTTCTA+TGG - chr5_1:20405778-20405797 None:intergenic 40.0%
ATTGAGGATGAAATTGGAGG+AGG - chr5_1:20405794-20405813 None:intergenic 40.0%
TACTTTGACAACGATAGAGC+CGG - chr5_1:20405907-20405926 None:intergenic 40.0%
GCAAGTATGAAGGTAAAAGC+AGG - chr5_1:20406106-20406125 None:intergenic 40.0%
ACCCAAGTCATAGATGTTAG+TGG + chr5_1:20406203-20406222 Msa0722890:intron 40.0%
AGGTCTAATCATATCCGATG+TGG + chr5_1:20406227-20406246 Msa0722890:intron 40.0%
TCCGATGTGGCAATTGTATT+TGG + chr5_1:20406240-20406259 Msa0722890:intron 40.0%
TGAACACAATGTGCACTTTC+AGG + chr5_1:20406666-20406685 Msa0722890:intron 40.0%
! TGCAGATTTTTGTGTGAACC+AGG + chr5_1:20407020-20407039 Msa0722890:intron 40.0%
GCACTTACCTTGCACTAAAT+TGG + chr5_1:20407096-20407115 Msa0722890:intron 40.0%
! AACAAGCATTTGTTGCAGCA+TGG + chr5_1:20407195-20407214 Msa0722890:CDS 40.0%
AATTGTAGCTCTCATTGCAG+AGG + chr5_1:20407234-20407253 Msa0722890:CDS 40.0%
ATTGTAGCTCTCATTGCAGA+GGG + chr5_1:20407235-20407254 Msa0722890:CDS 40.0%
TTTGGATTCTCAGACCTGAT+AGG + chr5_1:20407269-20407288 Msa0722890:CDS 40.0%
TTGGATTCTCAGACCTGATA+GGG + chr5_1:20407270-20407289 Msa0722890:CDS 40.0%
CATTGAAAGGTCAATGGTTC+AGG + chr5_1:20407392-20407411 Msa0722890:intron 40.0%
CACACATAGAGTTTGGGAAA+GGG - chr5_1:20407487-20407506 None:intergenic 40.0%
TCACACATAGAGTTTGGGAA+AGG - chr5_1:20407488-20407507 None:intergenic 40.0%
AGCTCTCACACATAGAGTTT+GGG - chr5_1:20407493-20407512 None:intergenic 40.0%
AAGCTCTCACACATAGAGTT+TGG - chr5_1:20407494-20407513 None:intergenic 40.0%
TGCATAGAAAAAGGGCAACA+TGG - chr5_1:20407524-20407543 None:intergenic 40.0%
TAACCAATGTGGAGACTTTG+TGG + chr5_1:20407931-20407950 Msa0722890:intron 40.0%
!! TGAGGAGTGTTGTATACACA+TGG + chr5_1:20407988-20408007 Msa0722890:CDS 40.0%
!! GAGGAGTGTTGTATACACAT+GGG + chr5_1:20407989-20408008 Msa0722890:CDS 40.0%
GTAGCTATGTTCAACCCATT+AGG + chr5_1:20408038-20408057 Msa0722890:CDS 40.0%
GTGCAATGACCATTCCTAAT+GGG - chr5_1:20408055-20408074 None:intergenic 40.0%
AGTGCAATGACCATTCCTAA+TGG - chr5_1:20408056-20408075 None:intergenic 40.0%
TTAGGAATGGTCATTGCACT+TGG + chr5_1:20408056-20408075 Msa0722890:CDS 40.0%
TTGGGAGACAGTCTTTATCT+TGG + chr5_1:20408095-20408114 Msa0722890:CDS 40.0%
GAGACAGTCTTTATCTTGGA+AGG + chr5_1:20408099-20408118 Msa0722890:CDS 40.0%
!!! ATTTTGGTTGACAACAGAGG+GGG - chr5_1:20408362-20408381 None:intergenic 40.0%
!!! CCTATTTTGGTTGACAACAG+AGG - chr5_1:20408365-20408384 None:intergenic 40.0%
CCTCTGTTGTCAACCAAAAT+AGG + chr5_1:20408362-20408381 Msa0722890:CDS 40.0%
GGAGTTGTCCTTGTTAAGTA+AGG - chr5_1:20408433-20408452 None:intergenic 40.0%
GAAAAATTGAGTCCCCGTTA+AGG + chr5_1:20408479-20408498 Msa0722890:three_prime_UTR 40.0%
TAGTTGTCTGTTCCTTAACG+GGG - chr5_1:20408494-20408513 None:intergenic 40.0%
TATAAGGACATTCTCTCTCC+TGG + chr5_1:20408514-20408533 Msa0722890:three_prime_UTR 40.0%
ATAAGGACATTCTCTCTCCT+GGG + chr5_1:20408515-20408534 Msa0722890:three_prime_UTR 40.0%
AGTGAGAGCAAGGAAATTGG+AGG + chr5_1:20405575-20405594 Msa0722890:five_prime_UTR 45.0%
GGAGGAAAAATGGTGATCGA+AGG + chr5_1:20405593-20405612 Msa0722890:exon 45.0%
GTTCAATTCCTAGAAGTGGG+TGG + chr5_1:20405644-20405663 Msa0722890:CDS 45.0%
GATTGATCTTGAGCTACCTG+TGG + chr5_1:20405993-20406012 Msa0722890:intron 45.0%
AGCATAGCAGATGCTAGTGT+GGG - chr5_1:20406073-20406092 None:intergenic 45.0%
AAGCATAGCAGATGCTAGTG+TGG - chr5_1:20406074-20406093 None:intergenic 45.0%
TGAAGGTAAAAGCAGGGAGA+AGG - chr5_1:20406099-20406118 None:intergenic 45.0%
CACAATGTGCACTTTCAGGA+TGG + chr5_1:20406670-20406689 Msa0722890:intron 45.0%
GGATTATCCTGAAGAGCTAG+TGG + chr5_1:20407171-20407190 Msa0722890:CDS 45.0%
! CTTGTTACCACTAGCTCTTC+AGG - chr5_1:20407181-20407200 None:intergenic 45.0%
! AAGCATTTGTTGCAGCATGG+TGG + chr5_1:20407198-20407217 Msa0722890:CDS 45.0%
CAACCACAAAGTCTCCACAT+TGG - chr5_1:20407937-20407956 None:intergenic 45.0%
ACATGGGCATTCCGTAAGAA+AGG + chr5_1:20408005-20408024 Msa0722890:CDS 45.0%
AATGGTCATTGCACTTGGCA+TGG + chr5_1:20408061-20408080 Msa0722890:CDS 45.0%
ATGGTCATTGCACTTGGCAT+GGG + chr5_1:20408062-20408081 Msa0722890:CDS 45.0%
GGAGCTGCTATAATAGCCAT+TGG + chr5_1:20408275-20408294 Msa0722890:CDS 45.0%
GTCAACCAAAATAGGTGCCT+AGG + chr5_1:20408370-20408389 Msa0722890:CDS 45.0%
!! TGTAAGAGCATCTCCAATGG+AGG + chr5_1:20408405-20408424 Msa0722890:three_prime_UTR 45.0%
!! GTAAGAGCATCTCCAATGGA+GGG + chr5_1:20408406-20408425 Msa0722890:three_prime_UTR 45.0%
GTTAAGTAAGGACCCTCCAT+TGG - chr5_1:20408421-20408440 None:intergenic 45.0%
TGGAGGGTCCTTACTTAACA+AGG + chr5_1:20408422-20408441 Msa0722890:three_prime_UTR 45.0%
TCTCTCTCCTGGGTAACTAA+CGG + chr5_1:20408525-20408544 Msa0722890:three_prime_UTR 45.0%
!! AATAAATATTATTATGTATA+TGG + chr5_1:20406359-20406378 Msa0722890:intron 5.0%
TTGAAGTGGGAGTGAGAGCA+AGG + chr5_1:20405565-20405584 Msa0722890:five_prime_UTR 50.0%
GGGAGTGAGAGCAAGGAAAT+TGG + chr5_1:20405572-20405591 Msa0722890:five_prime_UTR 50.0%
GAGTGTTACCACCCACTTCT+AGG - chr5_1:20405655-20405674 None:intergenic 50.0%
GATAGAGCCGGAGGATTTGA+AGG - chr5_1:20405895-20405914 None:intergenic 50.0%
TTTGACAACGATAGAGCCGG+AGG - chr5_1:20405904-20405923 None:intergenic 50.0%
CACAGTTAATGCCCCTGCAA+TGG - chr5_1:20406753-20406772 None:intergenic 50.0%
AATCTCTCTGCAGTTGCACG+TGG - chr5_1:20407052-20407071 None:intergenic 50.0%
TCTCAGACCTGATAGGGAGT+TGG + chr5_1:20407276-20407295 Msa0722890:CDS 50.0%
ACAGCTACCAACTCCCTATC+AGG - chr5_1:20407286-20407305 None:intergenic 50.0%
AAGGTCAATGGTTCAGGTCG+TGG + chr5_1:20407398-20407417 Msa0722890:intron 50.0%
! AAAGCGCTCCTGCTAAAAGC+TGG - chr5_1:20407787-20407806 None:intergenic 50.0%
TATGTGGGCACAAGCTCAAG+AGG + chr5_1:20408310-20408329 Msa0722890:CDS 50.0%
! AGTCGCCTAGGCACCTATTT+TGG - chr5_1:20408378-20408397 None:intergenic 50.0%
ACAAGGACAACTCCCCGTTA+AGG + chr5_1:20408439-20408458 Msa0722890:three_prime_UTR 50.0%
TCCAATGGAGGGTCCTTAAC+GGG - chr5_1:20408455-20408474 None:intergenic 50.0%
CTCCAATGGAGGGTCCTTAA+CGG - chr5_1:20408456-20408475 None:intergenic 50.0%
CTCTCTCCTGGGTAACTAAC+GGG + chr5_1:20408526-20408545 Msa0722890:three_prime_UTR 50.0%
CTGCAGTACTGCACAACCAC+AGG - chr5_1:20406012-20406031 None:intergenic 55.0%
GCACAGTGGTCTCCATTGCA+GGG + chr5_1:20406738-20406757 Msa0722890:CDS 55.0%
CACAGTGGTCTCCATTGCAG+GGG + chr5_1:20406739-20406758 Msa0722890:CDS 55.0%
!! AGTTGCACGTGGTGGATACC+TGG - chr5_1:20407041-20407060 None:intergenic 55.0%
CCAATGGAGGGTCCTTAACG+GGG - chr5_1:20408454-20408473 None:intergenic 55.0%
CGTTAAGGACCCTCCATTGG+AGG + chr5_1:20408454-20408473 Msa0722890:three_prime_UTR 55.0%
GGCACAGTGGTCTCCATTGC+AGG + chr5_1:20406737-20406756 Msa0722890:CDS 60.0%
CTCTCTGCAGTTGCACGTGG+TGG - chr5_1:20407049-20407068 None:intergenic 60.0%
CCCCGTTAAGGACCCTCCAT+TGG + chr5_1:20408451-20408470 Msa0722890:three_prime_UTR 60.0%
GTGGGACCCGTTAGTTACCC+AGG - chr5_1:20408535-20408554 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 20405533 20408758 20405533 ID=Msa0722890;Name=Msa0722890
chr5_1 mRNA 20405533 20408758 20405533 ID=Msa0722890-mRNA-1;Parent=Msa0722890;Name=Msa0722890-mRNA-1;_AED=0.37;_eAED=0.37;_QI=69|1|1|1|1|1|6|367|344
chr5_1 exon 20405533 20405846 20405533 ID=Msa0722890-mRNA-1:exon:7769;Parent=Msa0722890-mRNA-1
chr5_1 exon 20406024 20406137 20406024 ID=Msa0722890-mRNA-1:exon:7770;Parent=Msa0722890-mRNA-1
chr5_1 exon 20406688 20406928 20406688 ID=Msa0722890-mRNA-1:exon:7771;Parent=Msa0722890-mRNA-1
chr5_1 exon 20407157 20407315 20407157 ID=Msa0722890-mRNA-1:exon:7772;Parent=Msa0722890-mRNA-1
chr5_1 exon 20407969 20408120 20407969 ID=Msa0722890-mRNA-1:exon:7773;Parent=Msa0722890-mRNA-1
chr5_1 exon 20408268 20408758 20408268 ID=Msa0722890-mRNA-1:exon:7774;Parent=Msa0722890-mRNA-1
chr5_1 five_prime_UTR 20405533 20405601 20405533 ID=Msa0722890-mRNA-1:five_prime_utr;Parent=Msa0722890-mRNA-1
chr5_1 CDS 20405602 20405846 20405602 ID=Msa0722890-mRNA-1:cds;Parent=Msa0722890-mRNA-1
chr5_1 CDS 20406024 20406137 20406024 ID=Msa0722890-mRNA-1:cds;Parent=Msa0722890-mRNA-1
chr5_1 CDS 20406688 20406928 20406688 ID=Msa0722890-mRNA-1:cds;Parent=Msa0722890-mRNA-1
chr5_1 CDS 20407157 20407315 20407157 ID=Msa0722890-mRNA-1:cds;Parent=Msa0722890-mRNA-1
chr5_1 CDS 20407969 20408120 20407969 ID=Msa0722890-mRNA-1:cds;Parent=Msa0722890-mRNA-1
chr5_1 CDS 20408268 20408391 20408268 ID=Msa0722890-mRNA-1:cds;Parent=Msa0722890-mRNA-1
chr5_1 three_prime_UTR 20408392 20408758 20408392 ID=Msa0722890-mRNA-1:three_prime_utr;Parent=Msa0722890-mRNA-1
Gene Sequence

>Msa0722890

ATGGTGATCGAAGGAGCAAGTGTAACAGCAACAATGGTTGCAGTTCAATTCCTAGAAGTGGGTGGTAACACTCTGATCAAATCAGCCACCAATGATGGAATGAGTATTTTTGTTTTCACTTTTTACTCAAACCTTTTTGCTCTATGTTTCCTTTTACCATGCACCTTTTTTTACCATAGAAAAAGATCTCCTCCTCCAATTTCATCCTCAATTTTATGTAGAATTTTTCTTCTTAGTTGCCTCAGTACTGCAGTGCAGATTTTAACGAATATTGGAATTGAATGTAGCTCTCCCACACTAGCATCTGCTATGCTTGACCTTCTCCCTGCTTTTACCTTCATACTTGCTTTAATTTCAAGGATGGAAAATCTAAATCTGAAACACCATAGCAGCCAAGCTAAAATAATTGGCACAGTGGTCTCCATTGCAGGGGCATTAACTGTGACATTATATAAAGGGATACCATTGATTAGTAACGCCTTTCAAAATATAGATATTGGAGGAAGTGGAATTCACCTTTCAGTTAAATCAGATTGGATAGTTGGTGCTTTTCTTCTTGCAACTGCTAGCTTTTGCCTTTCTGTTTTGTATATTGTCCAGACTTGGATTATAAGGGATTATCCTGAAGAGCTAGTGGTAACAAGCATTTGTTGCAGCATGGTGGTAATTCTATCTGCAATTGTAGCTCTCATTGCAGAGGGAAATTCAAAAGTTTGGATTCTCAGACCTGATAGGGAGTTGGTAGCTGTGTGTTATTCAGCAATTTGTGTGGTATCAATGAGGAGTGTTGTATACACATGGGCATTCCGTAAGAAAGGACCAATATATGTAGCTATGTTCAACCCATTAGGAATGGTCATTGCACTTGGCATGGGTGTAATATTTTTGGGAGACAGTCTTTATCTTGGAAGCATGATTGGAGCTGCTATAATAGCCATTGGATTTTATGCAGTTATGTGGGCACAAGCTCAAGAGGAAAACACAACATGTGAAAATAAATTTCCCCCTCTGTTGTCAACCAAAATAGGTGCCTAG
Protein sequence

>Msa0722890

MVIEGASVTATMVAVQFLEVGGNTLIKSATNDGMSIFVFTFYSNLFALCFLLPCTFFYHRKRSPPPISSSILCRIFLLSCLSTAVQILTNIGIECSSPTLASAMLDLLPAFTFILALISRMENLNLKHHSSQAKIIGTVVSIAGALTVTLYKGIPLISNAFQNIDIGGSGIHLSVKSDWIVGAFLLATASFCLSVLYIVQTWIIRDYPEELVVTSICCSMVVILSAIVALIAEGNSKVWILRPDRELVAVCYSAICVVSMRSVVYTWAFRKKGPIYVAMFNPLGMVIALGMGVIFLGDSLYLGSMIGAAIIAIGFYAVMWAQAQEENTTCENKFPPLLSTKIGA*