AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1449900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1449900 MtrunA17_Chr1g0210771 92.521 361 26 1 19 379 23 382 0.0 701
Msa1449900 MtrunA17_Chr1g0171171 70.526 380 106 4 1 379 1 375 0.0 569
Msa1449900 MtrunA17_Chr3g0105301 41.493 335 177 6 55 375 43 372 3.15e-94 286
Msa1449900 MtrunA17_Chr5g0430371 39.655 348 181 7 55 379 58 399 1.33e-92 283
Msa1449900 MtrunA17_Chr2g0283581 41.390 331 174 6 55 379 42 358 1.05e-90 277
Msa1449900 MtrunA17_Chr4g0006991 41.210 347 181 8 53 379 84 427 1.14e-87 271
Msa1449900 MtrunA17_Chr3g0097891 41.916 334 171 8 55 379 31 350 1.51e-87 268
Msa1449900 MtrunA17_Chr2g0325481 39.157 332 182 7 54 379 49 366 1.79e-87 269
Msa1449900 MtrunA17_Chr7g0258851 38.421 380 211 9 19 379 212 587 1.24e-86 274
Msa1449900 MtrunA17_Chr1g0175671 40.922 347 186 8 50 379 165 509 7.71e-86 270
Msa1449900 MtrunA17_Chr2g0314791 41.298 339 167 9 53 379 27 345 4.77e-84 259
Msa1449900 MtrunA17_Chr5g0413271 36.729 373 220 9 15 379 112 476 1.04e-82 260
Msa1449900 MtrunA17_Chr2g0314801 38.739 333 184 7 51 375 29 349 3.06e-82 255
Msa1449900 MtrunA17_Chr5g0413851 37.234 376 206 9 14 379 4 359 1.34e-81 253
Msa1449900 MtrunA17_Chr4g0041011 37.202 336 181 8 55 379 47 363 1.66e-80 251
Msa1449900 MtrunA17_Chr1g0213281 36.623 385 220 8 17 379 133 515 8.75e-80 254
Msa1449900 MtrunA17_Chr7g0269991 35.224 335 195 5 55 375 137 463 7.58e-79 250
Msa1449900 MtrunA17_Chr4g0071391 34.115 384 224 8 21 379 67 446 1.51e-77 246
Msa1449900 MtrunA17_Chr7g0242701 38.617 347 190 8 53 379 84 427 2.15e-77 244
Msa1449900 MtrunA17_Chr3g0113671 39.080 348 177 11 55 375 106 445 8.63e-77 244
Msa1449900 MtrunA17_Chr4g0045951 36.243 378 208 9 12 379 8 362 1.10e-76 241
Msa1449900 MtrunA17_Chr8g0360521 38.109 349 182 11 55 379 71 409 1.44e-76 242
Msa1449900 MtrunA17_Chr4g0045941 35.345 348 198 7 42 379 20 350 4.43e-76 239
Msa1449900 MtrunA17_Chr4g0005491 34.527 391 227 12 10 379 25 407 2.70e-75 239
Msa1449900 MtrunA17_Chr5g0437291 39.167 360 167 12 53 375 163 507 3.75e-75 241
Msa1449900 MtrunA17_Chr4g0068111 36.141 368 211 9 31 379 91 453 2.53e-74 238
Msa1449900 MtrunA17_Chr3g0091031 34.254 362 204 9 29 377 6 346 1.68e-72 229
Msa1449900 MtrunA17_Chr3g0085081 33.889 360 208 8 29 377 6 346 1.89e-72 229
Msa1449900 MtrunA17_Chr3g0114101 34.694 392 228 10 14 379 41 430 6.19e-72 231
Msa1449900 MtrunA17_Chr8g0368461 34.473 351 202 9 52 375 175 524 3.53e-71 232
Msa1449900 MtrunA17_Chr3g0085071 34.375 352 197 9 34 377 36 361 1.08e-70 226
Msa1449900 MtrunA17_Chr5g0437601 36.522 345 196 9 55 379 83 424 1.85e-69 224
Msa1449900 MtrunA17_Chr7g0243301 33.803 355 204 10 51 379 60 409 2.62e-68 221
Msa1449900 MtrunA17_Chr4g0005481 34.000 350 211 8 47 379 100 446 1.25e-67 220
Msa1449900 MtrunA17_Chr7g0259711 33.526 346 205 8 55 379 91 432 3.35e-61 203
Msa1449900 MtrunA17_Chr3g0126021 34.574 376 209 8 25 379 100 459 7.16e-61 203
Msa1449900 MtrunA17_Chr2g0287901 32.162 370 216 9 32 377 77 435 1.81e-60 201
Msa1449900 MtrunA17_Chr8g0384151 31.765 340 208 4 53 379 88 416 6.63e-57 192
Msa1449900 MtrunA17_Chr1g0209921 39.732 224 125 7 53 271 42 260 2.24e-53 177
Msa1449900 MtrunA17_Chr1g0178081 30.250 400 199 12 1 379 1 341 1.03e-51 176
Msa1449900 MtrunA17_Chr6g0476041 28.919 370 218 12 40 375 152 510 1.38e-41 152
Msa1449900 MtrunA17_Chr1g0188191 28.889 360 201 13 55 375 234 577 2.52e-37 142
Msa1449900 MtrunA17_Chr8g0347321 28.721 383 230 16 30 379 67 439 1.83e-34 132
Msa1449900 MtrunA17_Chr5g0414061 27.596 366 213 13 46 375 85 434 2.02e-33 129
Msa1449900 MtrunA17_Chr7g0226991 26.667 360 211 11 53 375 55 398 5.89e-33 127
Msa1449900 MtrunA17_Chr3g0140371 27.401 354 209 11 55 375 18 356 7.42e-33 126
Msa1449900 MtrunA17_Chr8g0384421 27.966 354 212 14 53 377 131 470 4.13e-32 126
Msa1449900 MtrunA17_Chr8g0391481 28.212 358 208 13 53 375 25 368 1.03e-31 123
Msa1449900 MtrunA17_Chr8g0387921 24.816 407 242 13 11 375 31 415 4.58e-31 122
Msa1449900 MtrunA17_Chr6g0456691 26.034 411 250 16 7 375 17 415 7.60e-31 122
Msa1449900 MtrunA17_Chr5g0414091 26.596 376 198 14 50 375 80 427 7.78e-31 122
Msa1449900 MtrunA17_Chr4g0049421 26.761 355 214 12 55 376 48 389 1.52e-30 120
Msa1449900 MtrunA17_Chr2g0283851 28.933 356 196 13 59 375 59 396 1.71e-30 120
Msa1449900 MtrunA17_Chr5g0414041 30.141 355 202 13 55 375 87 429 6.38e-30 119
Msa1449900 MtrunA17_Chr6g0451321 27.548 363 208 16 53 379 114 457 2.22e-29 118
Msa1449900 MtrunA17_Chr4g0070341 26.902 368 220 15 45 377 116 469 1.95e-27 112
Msa1449900 MtrunA17_Chr6g0456681 26.050 357 216 11 53 375 62 404 1.41e-26 109
Msa1449900 MtrunA17_Chr5g0414051 34.320 169 103 5 55 218 75 240 1.90e-25 106
Msa1449900 MtrunA17_Chr7g0221271 38.931 131 79 1 51 180 75 205 2.43e-24 99.4
Msa1449900 MtrunA17_Chr7g0221291 46.875 96 50 1 51 145 75 170 1.20e-23 96.3
Msa1449900 MtrunA17_Chr6g0455901 26.098 410 242 15 8 375 22 412 1.76e-22 98.2
Msa1449900 MtrunA17_Chr2g0287931 35.593 118 64 3 115 220 3 120 1.15e-15 73.2
Msa1449900 MtrunA17_Chr1g0209931 48.101 79 28 3 302 379 26 92 1.21e-13 66.6
Msa1449900 MtrunA17_Chr5g0429211 36.000 75 48 0 108 182 194 268 1.25e-11 64.7
Msa1449900 MtrunA17_Chr5g0429191 52.174 46 20 1 327 372 2 45 3.18e-11 58.2
Msa1449900 MtrunA17_Chr3g0085101 29.907 107 69 3 240 340 1 107 5.04e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1449900 AT3G54260.1 60.000 380 137 6 2 377 4 372 2.27e-167 473
Msa1449900 AT2G31110.2 37.978 366 198 9 21 375 11 358 2.05e-87 269
Msa1449900 AT5G58600.1 39.474 342 183 8 55 379 66 400 4.03e-86 267
Msa1449900 AT1G29050.1 39.701 335 175 6 55 379 61 378 7.47e-85 263
Msa1449900 AT2G30900.1 40.120 334 176 9 53 375 41 361 6.53e-84 260
Msa1449900 AT2G34070.1 38.348 339 187 7 49 379 59 383 2.80e-83 259
Msa1449900 AT5G06700.1 37.705 366 204 8 37 379 230 594 1.12e-82 264
Msa1449900 AT2G30010.1 39.481 347 182 9 55 379 56 396 1.77e-80 252
Msa1449900 AT5G01360.2 37.176 347 196 8 53 379 34 378 2.10e-80 251
Msa1449900 AT2G30010.2 39.481 347 182 9 55 379 99 439 2.65e-80 253
Msa1449900 AT5G01360.1 37.176 347 196 8 53 379 90 434 7.58e-80 251
Msa1449900 AT3G62390.1 36.795 337 194 6 53 375 136 467 1.18e-79 252
Msa1449900 AT1G78710.1 37.994 329 184 8 54 375 37 352 1.40e-79 248
Msa1449900 AT3G12060.1 38.286 350 191 9 50 379 191 535 8.96e-79 252
Msa1449900 AT1G73140.1 37.464 347 191 9 53 379 65 405 2.82e-78 247
Msa1449900 AT2G42570.1 38.209 335 182 8 54 379 46 364 5.12e-78 245
Msa1449900 AT5G20590.1 38.081 344 193 8 53 379 139 479 6.79e-78 248
Msa1449900 AT3G55990.1 37.027 370 202 10 28 375 119 479 3.37e-77 246
Msa1449900 AT5G49340.1 35.809 377 211 9 22 379 77 441 2.10e-76 243
Msa1449900 AT2G40150.1 37.784 352 184 10 54 375 70 416 6.77e-76 241
Msa1449900 AT1G60790.1 35.549 346 203 9 49 375 184 528 7.46e-75 242
Msa1449900 AT3G14850.2 35.629 334 193 7 55 379 34 354 2.05e-73 233
Msa1449900 AT5G01620.2 35.838 346 199 9 53 379 108 449 3.98e-73 234
Msa1449900 AT5G01620.1 35.838 346 199 9 53 379 108 449 3.98e-73 234
Msa1449900 AT5G01620.3 35.838 346 199 9 53 379 116 457 5.01e-73 234
Msa1449900 AT2G40160.2 36.599 347 198 10 55 379 77 423 2.46e-72 233
Msa1449900 AT2G40160.1 36.311 347 199 10 55 379 77 423 2.97e-72 232
Msa1449900 AT3G11030.1 36.443 343 200 7 55 379 106 448 1.73e-71 231
Msa1449900 AT2G40320.1 33.081 396 241 9 8 379 27 422 6.10e-68 221
Msa1449900 AT2G38320.1 31.017 403 242 11 3 379 12 404 3.61e-67 218
Msa1449900 AT2G31110.3 38.189 254 154 3 20 272 10 261 5.99e-66 210
Msa1449900 AT2G30900.2 43.243 222 121 5 53 271 41 260 2.76e-63 204
Msa1449900 AT1G48880.1 33.524 349 186 8 54 379 114 439 1.68e-61 204
Msa1449900 AT5G19160.1 32.800 375 215 9 33 377 75 442 4.01e-61 204
Msa1449900 AT5G58600.2 40.435 230 130 4 55 282 66 290 2.48e-60 197
Msa1449900 AT3G62390.2 39.301 229 128 3 53 270 136 364 3.24e-59 197
Msa1449900 AT3G06080.2 33.333 354 202 9 50 377 98 443 4.40e-59 199
Msa1449900 AT2G38320.2 32.209 326 192 8 78 379 1 321 2.46e-57 190
Msa1449900 AT5G06230.2 33.144 353 203 8 53 379 19 364 8.77e-57 190
Msa1449900 AT5G06230.1 33.144 353 203 8 53 379 60 405 1.46e-56 191
Msa1449900 AT2G34070.2 36.882 263 145 6 124 379 1 249 7.01e-56 184
Msa1449900 AT1G78710.2 31.461 356 176 10 30 379 3 296 1.32e-55 185
Msa1449900 AT3G11570.1 31.486 397 232 13 8 379 38 419 9.39e-55 186
Msa1449900 AT2G40160.3 38.767 227 128 6 55 270 77 303 5.78e-52 176
Msa1449900 AT5G20590.2 39.130 230 127 5 53 269 139 368 1.30e-51 177
Msa1449900 AT5G19160.2 35.443 237 140 4 50 273 94 330 3.61e-46 161
Msa1449900 AT3G06080.1 34.177 237 143 4 50 273 98 334 3.99e-44 156
Msa1449900 AT3G14850.1 33.462 260 152 6 124 375 1 247 5.57e-44 153
Msa1449900 AT2G31110.1 39.450 218 108 7 166 375 9 210 1.43e-43 150
Msa1449900 AT5G06230.3 33.333 240 141 4 53 273 60 299 2.59e-41 147
Msa1449900 AT1G70230.1 30.372 349 203 12 55 379 79 411 1.34e-37 140
Msa1449900 AT5G51640.1 28.912 377 224 13 31 375 120 484 2.24e-37 141
Msa1449900 AT4G25360.1 28.612 353 215 11 54 375 174 520 1.39e-36 139
Msa1449900 AT4G25360.2 28.612 353 215 11 54 375 174 520 1.39e-36 139
Msa1449900 AT1G01430.1 25.337 371 240 12 35 375 80 443 4.42e-33 129
Msa1449900 AT4G01080.1 27.841 352 215 12 51 375 87 426 8.87e-33 127
Msa1449900 AT5G64020.1 27.042 355 222 15 55 377 57 406 9.36e-33 127
Msa1449900 AT3G28150.1 28.256 407 214 18 12 377 39 408 2.32e-31 123
Msa1449900 AT5G15890.1 27.557 352 216 10 49 375 181 518 3.77e-31 124
Msa1449900 AT5G20680.6 27.249 389 227 15 26 379 90 457 6.02e-31 123
Msa1449900 AT2G37720.1 26.667 360 213 15 53 379 141 482 9.93e-31 122
Msa1449900 AT5G20680.4 27.249 389 227 15 26 379 184 551 1.07e-30 123
Msa1449900 AT5G20680.1 27.249 389 227 15 26 379 184 551 1.07e-30 123
Msa1449900 AT5G20680.3 27.249 389 227 15 26 379 184 551 1.07e-30 123
Msa1449900 AT5G20680.5 27.249 389 227 15 26 379 184 551 1.07e-30 123
Msa1449900 AT5G20680.2 27.249 389 227 15 26 379 166 533 1.11e-30 123
Msa1449900 AT5G15900.1 29.899 398 201 18 26 379 46 409 3.97e-30 120
Msa1449900 AT5G15900.2 29.899 398 201 18 26 379 65 428 5.33e-30 120
Msa1449900 AT4G11090.1 28.019 414 246 18 1 375 21 421 1.09e-29 119
Msa1449900 AT5G64470.3 25.862 348 221 11 55 376 55 391 1.23e-28 115
Msa1449900 AT5G64470.2 26.271 354 218 13 55 376 55 397 6.52e-28 114
Msa1449900 AT3G02440.2 28.205 351 209 13 55 375 135 472 2.98e-27 112
Msa1449900 AT3G02440.1 28.205 351 209 13 55 375 127 464 3.14e-27 112
Msa1449900 AT2G14530.1 26.184 359 217 10 55 379 62 406 7.12e-27 110
Msa1449900 AT4G23790.1 26.593 361 213 14 53 375 77 423 1.25e-26 110
Msa1449900 AT5G64470.4 26.471 238 153 8 55 275 55 287 4.15e-19 87.4
Msa1449900 AT5G64470.1 26.471 238 153 8 55 275 55 287 4.81e-19 87.4

Find 96 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCTGGTGTGCCTGATATT+TGG 0.189016 tig0032745:+6540 Msa1449900:CDS
GTGATTTCTCAGTTGGAAAA+TGG 0.208372 tig0032745:+5125 Msa1449900:CDS
AGACATCAATTGAGTTCTTC+TGG 0.226253 tig0032745:+5761 Msa1449900:CDS
ATTTCTTCCATGAATCTCTT+TGG 0.226552 tig0032745:-5102 None:intergenic
GCTCATTGGTGGACTCATTC+TGG 0.278727 tig0032745:+5901 Msa1449900:CDS
TGATTAAATTGTAGGCAAAA+TGG 0.281278 tig0032745:+6275 Msa1449900:intron
AAAGTGGGTGGATTTGAATT+TGG 0.291594 tig0032745:+6124 Msa1449900:CDS
TTTCTTCCATGAATCTCTTT+GGG 0.298282 tig0032745:-5101 None:intergenic
AGCATTTCGTTCCAAATATC+AGG 0.327867 tig0032745:-6551 None:intergenic
TTCAGCCACATGCATGTTCC+AGG 0.356693 tig0032745:+6335 Msa1449900:CDS
GTAATATCCTGTAGATATAC+TGG 0.367331 tig0032745:-6401 None:intergenic
GGACCAGTAGTAGTGCTAAA+AGG 0.371991 tig0032745:+6356 Msa1449900:CDS
TAATAAGAGAATTCTACATT+TGG 0.381121 tig0032745:+5819 Msa1449900:CDS
TTGAAGAGAATGCAAGGTAT+TGG 0.381566 tig0032745:+5848 Msa1449900:CDS
ATAGCATGGAAAGACATTAA+AGG 0.392314 tig0032745:-5653 None:intergenic
TTCTTCCAATGCAAGCCAAC+TGG 0.394214 tig0032745:-5053 None:intergenic
TGCAGCCATTGGTGCCTCCC+TGG 0.399442 tig0032745:+6524 Msa1449900:CDS
TTTAATGTCTTTCCATGCTA+TGG 0.404785 tig0032745:+5655 Msa1449900:CDS
TTGCATTTGATTCAGCTCAT+TGG 0.406976 tig0032745:+5887 Msa1449900:CDS
TATTCCAACTGACAGGAAAA+AGG 0.407152 tig0032745:+5616 Msa1449900:CDS
TTCTTCAGTTCTACCAATAG+TGG 0.407885 tig0032745:-5787 None:intergenic
TGTATTGAGTCCTTTCTCAT+AGG 0.414458 tig0032745:-6096 None:intergenic
GTTCTTCTGGGCACCACTAT+TGG 0.416633 tig0032745:+5774 Msa1449900:CDS
CATTTGATTCAGCTCATTGG+TGG 0.419213 tig0032745:+5890 Msa1449900:CDS
GACATCAATTGAGTTCTTCT+GGG 0.424420 tig0032745:+5762 Msa1449900:CDS
AGCCAACTGGACACTTCCTC+TGG 0.424797 tig0032745:-5040 None:intergenic
AAGAAATGTGATTTCTCAGT+TGG 0.430768 tig0032745:+5118 Msa1449900:CDS
TTTGATGCACTGAAGTATCT+TGG 0.440296 tig0032745:+5506 Msa1449900:CDS
AAATGGAAGTGGAAGCCATT+AGG 0.444002 tig0032745:+5247 Msa1449900:CDS
AGACATTAAAGGACCATCAT+AGG 0.444756 tig0032745:-5642 None:intergenic
CATTTCTCATAATCTGAATC+CGG 0.445205 tig0032745:-5229 None:intergenic
CTTGGGATGGAACAACCTAA+TGG 0.446475 tig0032745:-5262 None:intergenic
AGTCCTCTGATTGCAGCCAT+TGG 0.458504 tig0032745:+6513 Msa1449900:CDS
CGGATTCAGATTATGAGAAA+TGG 0.459664 tig0032745:+5230 Msa1449900:CDS
TTCTATAATGAGAAACCAAT+GGG 0.459846 tig0032745:+5565 Msa1449900:CDS
GTATGGCACCTAGACATAAC+AGG 0.470822 tig0032745:+6176 Msa1449900:CDS
AGTGTCCAGTTGGCTTGCAT+TGG 0.472840 tig0032745:+5048 Msa1449900:CDS
TTCCAAATATCAGGCACACC+AGG 0.474779 tig0032745:-6542 None:intergenic
TGCAAACCCAAAGAGATTCA+TGG 0.480389 tig0032745:+5095 Msa1449900:CDS
TCTCTTGTATGCTTAGTTCA+AGG 0.493508 tig0032745:+5590 Msa1449900:CDS
CATCACAAACATGAATCAAA+TGG 0.494804 tig0032745:+6070 Msa1449900:CDS
CAAATGGTTGCCTATGAGAA+AGG 0.503172 tig0032745:+6086 Msa1449900:CDS
GAGATTTCCAGTATATCTAC+AGG 0.504762 tig0032745:+6394 Msa1449900:CDS
GCAGTAACTTGTCAAAAGAA+TGG 0.505516 tig0032745:+5202 Msa1449900:CDS
GTTGGAAATGTTACAATCAA+AGG 0.507595 tig0032745:+6297 Msa1449900:CDS
TAAATTGTAGGCAAAATGGT+TGG 0.508760 tig0032745:+6279 Msa1449900:intron
ATGAGAAAGGACTCAATACA+TGG 0.509591 tig0032745:+6099 Msa1449900:CDS
TTTAGCACTACTACTGGTCC+TGG 0.509768 tig0032745:-6353 None:intergenic
TATTGGTAGAACTGAAGAAA+GGG 0.514662 tig0032745:+5791 Msa1449900:CDS
AAGGAGTTATTCCAACTGAC+AGG 0.516555 tig0032745:+5609 Msa1449900:CDS
TACCAGGTGGGATTATTACA+TGG 0.524110 tig0032745:+6034 Msa1449900:intron
GCACACCAGGGAGGCACCAA+TGG 0.532334 tig0032745:-6529 None:intergenic
GTTACTGCAGTGGCGAGATA+AGG 0.535012 tig0032745:-5187 None:intergenic
CAGATTATGAGAAATGGAAG+TGG 0.537613 tig0032745:+5236 Msa1449900:CDS
CTATTGGTAGAACTGAAGAA+AGG 0.537856 tig0032745:+5790 Msa1449900:CDS
ACATCCTTCAGTGTATAGTA+AGG 0.542161 tig0032745:+6451 Msa1449900:CDS
AGCATACAAGAGATTCCCAT+TGG 0.544133 tig0032745:-5580 None:intergenic
GACTCAATACATGGGCAAAG+TGG 0.544827 tig0032745:+6108 Msa1449900:CDS
ATGACTGCATTTAGAAGAGA+TGG 0.549263 tig0032745:+6428 Msa1449900:CDS
GAGAGGCAGAAACAAGGTAA+TGG 0.549704 tig0032745:+6488 Msa1449900:CDS
ATTCTATAATGAGAAACCAA+TGG 0.552968 tig0032745:+5564 Msa1449900:CDS
ATTAATATCCTACCATAGCA+TGG 0.553143 tig0032745:-5667 None:intergenic
CATTAGGTTGTTCCATCCCA+AGG 0.558505 tig0032745:+5263 Msa1449900:CDS
AGGTAATAAGTGAAGAAGAG+AGG 0.559968 tig0032745:+6471 Msa1449900:CDS
GACAGTTGGAATCATAGAGA+GGG 0.562013 tig0032745:-5165 None:intergenic
AGTACGTACCTGTTATGTCT+AGG 0.567486 tig0032745:-6184 None:intergenic
GCACCAATGGCTGCAATCAG+AGG 0.571338 tig0032745:-6516 None:intergenic
GTTTCATCATCACCTTGGGA+TGG 0.572029 tig0032745:-5275 None:intergenic
TTGTCAAAAGAATGGACGGC+CGG 0.574038 tig0032745:+5210 Msa1449900:CDS
GGAAAGAGAATGATGTTGGT+GGG 0.575995 tig0032745:+5539 Msa1449900:CDS
ACTCAATACATGGGCAAAGT+GGG 0.582566 tig0032745:+6109 Msa1449900:CDS
GAGAGGAAAGAGAATGATGT+TGG 0.587409 tig0032745:+5535 Msa1449900:CDS
AATGGCTGCAATCAGAGGAC+TGG 0.587580 tig0032745:-6511 None:intergenic
GAGAATGCAAGGTATTGGAA+AGG 0.589108 tig0032745:+5853 Msa1449900:CDS
ATCCAGAGGAAGTGTCCAGT+TGG 0.594248 tig0032745:+5038 Msa1449900:CDS
ATTTGATTGAAGAGAATGCA+AGG 0.595081 tig0032745:+5842 Msa1449900:CDS
AAGTATCTTGGTAAAATGAG+AGG 0.596417 tig0032745:+5518 Msa1449900:CDS
AGGTGGGATTATTACATGGA+AGG 0.598017 tig0032745:+6038 Msa1449900:intron
CAATACATGGGCAAAGTGGG+TGG 0.598037 tig0032745:+6112 Msa1449900:CDS
ATTACCTTACTATACACTGA+AGG 0.598619 tig0032745:-6455 None:intergenic
GTGTATGTGGATAAGAAGGA+AGG 0.606097 tig0032745:-6737 None:intergenic
GTTGGAATCATAGAGAGGGT+AGG 0.612157 tig0032745:-5161 None:intergenic
TAACTTGTCAAAAGAATGGA+CGG 0.614689 tig0032745:+5206 Msa1449900:CDS
GGAACGAAATGCTGAGTGCA+TGG 0.615044 tig0032745:+6561 Msa1449900:CDS
GGATTGTAAGATCAGTAAGT+CGG 0.632612 tig0032745:-6716 None:intergenic
GAAGAAGAGAGGCAGAAACA+AGG 0.633633 tig0032745:+6482 Msa1449900:CDS
GGACAGTTGGAATCATAGAG+AGG 0.634353 tig0032745:-5166 None:intergenic
TCCAAATATCAGGCACACCA+GGG 0.635948 tig0032745:-6541 None:intergenic
AGTGGCGAGATAAGGACAGT+TGG 0.638580 tig0032745:-5179 None:intergenic
AGGAAAGAGAATGATGTTGG+TGG 0.644337 tig0032745:+5538 Msa1449900:CDS
AGTAGTAGTGCTAAAAGGAG+TGG 0.649261 tig0032745:+6361 Msa1449900:CDS
TGAGAAAGGACTCAATACAT+GGG 0.677756 tig0032745:+6100 Msa1449900:CDS
CTGGTCCTGGAACATGCATG+TGG 0.678164 tig0032745:-6340 None:intergenic
ATTATCACTGATCAATCCAG+AGG 0.696073 tig0032745:+5024 Msa1449900:CDS
AAATATCAGGCACACCAGGG+AGG 0.749580 tig0032745:-6538 None:intergenic
AGAAACTGAAGATGTCAACA+TGG 0.749769 tig0032745:+5072 Msa1449900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGAATACATTTACATAAA+AGG - tig0032745:5398-5417 None:intergenic 15.0%
!! CAAAAATAAAATATTGATGA+AGG - tig0032745:6224-6243 None:intergenic 15.0%
!! TTTAACTATAACTAAAAAGA+TGG + tig0032745:6585-6604 Msa1449900:exon 15.0%
!! TTTGAATACATTTACATAAA+AGG - tig0032745:5398-5417 None:intergenic 15.0%
!! CAAAAATAAAATATTGATGA+AGG - tig0032745:6224-6243 None:intergenic 15.0%
!! TTTAACTATAACTAAAAAGA+TGG + tig0032745:6585-6604 Msa1449900:exon 15.0%
!! ATAAAAATGACAGTAAATGA+AGG - tig0032745:4979-4998 None:intergenic 20.0%
!!! CATTTTTAGAAGGAAAATTA+AGG - tig0032745:5704-5723 None:intergenic 20.0%
!!! GTAAGTAAATCATTTTTAGA+AGG - tig0032745:5714-5733 None:intergenic 20.0%
!! TAATAAGAGAATTCTACATT+TGG + tig0032745:5819-5838 Msa1449900:CDS 20.0%
!!! TAATCATTTTTACATCCTAA+TGG + tig0032745:5995-6014 Msa1449900:intron 20.0%
!! AGAACATTTGATTAAATTGT+AGG + tig0032745:6267-6286 Msa1449900:intron 20.0%
!! ATAAAAATGACAGTAAATGA+AGG - tig0032745:4979-4998 None:intergenic 20.0%
!!! CATTTTTAGAAGGAAAATTA+AGG - tig0032745:5704-5723 None:intergenic 20.0%
!!! GTAAGTAAATCATTTTTAGA+AGG - tig0032745:5714-5733 None:intergenic 20.0%
!! TAATAAGAGAATTCTACATT+TGG + tig0032745:5819-5838 Msa1449900:CDS 20.0%
!!! TAATCATTTTTACATCCTAA+TGG + tig0032745:5995-6014 Msa1449900:intron 20.0%
!! AGAACATTTGATTAAATTGT+AGG + tig0032745:6267-6286 Msa1449900:intron 20.0%
!!! CTGTCATTTTTATTCTTCTT+AGG + tig0032745:4986-5005 Msa1449900:CDS 25.0%
! AAGCATGTAAGAAAACAAAA+AGG - tig0032745:5321-5340 None:intergenic 25.0%
! ATTCTATAATGAGAAACCAA+TGG + tig0032745:5564-5583 Msa1449900:CDS 25.0%
! TTCTATAATGAGAAACCAAT+GGG + tig0032745:5565-5584 Msa1449900:CDS 25.0%
!! TAATCAAGATTGATTCCATT+AGG - tig0032745:6013-6032 None:intergenic 25.0%
! AATCAATCTTGATTAATACC+AGG + tig0032745:6018-6037 Msa1449900:intron 25.0%
! TGATTAAATTGTAGGCAAAA+TGG + tig0032745:6275-6294 Msa1449900:intron 25.0%
! CTATAACTAAAAAGATGGAA+TGG + tig0032745:6590-6609 Msa1449900:three_prime_UTR 25.0%
!!! CTGTCATTTTTATTCTTCTT+AGG + tig0032745:4986-5005 Msa1449900:CDS 25.0%
! AAGCATGTAAGAAAACAAAA+AGG - tig0032745:5321-5340 None:intergenic 25.0%
! ATTCTATAATGAGAAACCAA+TGG + tig0032745:5564-5583 Msa1449900:CDS 25.0%
! TTCTATAATGAGAAACCAAT+GGG + tig0032745:5565-5584 Msa1449900:CDS 25.0%
!! TAATCAAGATTGATTCCATT+AGG - tig0032745:6013-6032 None:intergenic 25.0%
! AATCAATCTTGATTAATACC+AGG + tig0032745:6018-6037 Msa1449900:intron 25.0%
! TGATTAAATTGTAGGCAAAA+TGG + tig0032745:6275-6294 Msa1449900:intron 25.0%
! CTATAACTAAAAAGATGGAA+TGG + tig0032745:6590-6609 Msa1449900:three_prime_UTR 25.0%
TTTCTTCCATGAATCTCTTT+GGG - tig0032745:5104-5123 None:intergenic 30.0%
ATTTCTTCCATGAATCTCTT+TGG - tig0032745:5105-5124 None:intergenic 30.0%
AAGAAATGTGATTTCTCAGT+TGG + tig0032745:5118-5137 Msa1449900:CDS 30.0%
! TAACTTGTCAAAAGAATGGA+CGG + tig0032745:5206-5225 Msa1449900:CDS 30.0%
CATTTCTCATAATCTGAATC+CGG - tig0032745:5232-5251 None:intergenic 30.0%
TAATGTTTCATCATCACCTT+GGG - tig0032745:5282-5301 None:intergenic 30.0%
TTAATGTTTCATCATCACCT+TGG - tig0032745:5283-5302 None:intergenic 30.0%
! TGCAACATTTTTGACATGTA+TGG + tig0032745:5345-5364 Msa1449900:intron 30.0%
AGAAGCTAAATTTATCAGCA+TGG - tig0032745:5470-5489 None:intergenic 30.0%
AAGTATCTTGGTAAAATGAG+AGG + tig0032745:5518-5537 Msa1449900:CDS 30.0%
ATAGCATGGAAAGACATTAA+AGG - tig0032745:5656-5675 None:intergenic 30.0%
TTTAATGTCTTTCCATGCTA+TGG + tig0032745:5655-5674 Msa1449900:CDS 30.0%
ATTAATATCCTACCATAGCA+TGG - tig0032745:5670-5689 None:intergenic 30.0%
ACATGTTGTTCACTTATTAC+AGG + tig0032745:5732-5751 Msa1449900:intron 30.0%
TATTGGTAGAACTGAAGAAA+GGG + tig0032745:5791-5810 Msa1449900:CDS 30.0%
ATTTGATTGAAGAGAATGCA+AGG + tig0032745:5842-5861 Msa1449900:CDS 30.0%
AATCTTGATTAATACCAGGT+GGG + tig0032745:6022-6041 Msa1449900:intron 30.0%
CATCACAAACATGAATCAAA+TGG + tig0032745:6070-6089 Msa1449900:CDS 30.0%
TAAATTGTAGGCAAAATGGT+TGG + tig0032745:6279-6298 Msa1449900:intron 30.0%
GTTGGAAATGTTACAATCAA+AGG + tig0032745:6297-6316 Msa1449900:CDS 30.0%
! ATGTGGCTGAAATATTTTAC+AGG - tig0032745:6326-6345 None:intergenic 30.0%
GTAATATCCTGTAGATATAC+TGG - tig0032745:6404-6423 None:intergenic 30.0%
ATTACCTTACTATACACTGA+AGG - tig0032745:6458-6477 None:intergenic 30.0%
!!! GAATGGATCTGAGTTTTTTA+TGG + tig0032745:6607-6626 Msa1449900:three_prime_UTR 30.0%
TTTCTTCCATGAATCTCTTT+GGG - tig0032745:5104-5123 None:intergenic 30.0%
ATTTCTTCCATGAATCTCTT+TGG - tig0032745:5105-5124 None:intergenic 30.0%
AAGAAATGTGATTTCTCAGT+TGG + tig0032745:5118-5137 Msa1449900:CDS 30.0%
! TAACTTGTCAAAAGAATGGA+CGG + tig0032745:5206-5225 Msa1449900:CDS 30.0%
CATTTCTCATAATCTGAATC+CGG - tig0032745:5232-5251 None:intergenic 30.0%
TAATGTTTCATCATCACCTT+GGG - tig0032745:5282-5301 None:intergenic 30.0%
TTAATGTTTCATCATCACCT+TGG - tig0032745:5283-5302 None:intergenic 30.0%
! TGCAACATTTTTGACATGTA+TGG + tig0032745:5345-5364 Msa1449900:intron 30.0%
AGAAGCTAAATTTATCAGCA+TGG - tig0032745:5470-5489 None:intergenic 30.0%
AAGTATCTTGGTAAAATGAG+AGG + tig0032745:5518-5537 Msa1449900:CDS 30.0%
ATAGCATGGAAAGACATTAA+AGG - tig0032745:5656-5675 None:intergenic 30.0%
TTTAATGTCTTTCCATGCTA+TGG + tig0032745:5655-5674 Msa1449900:CDS 30.0%
ATTAATATCCTACCATAGCA+TGG - tig0032745:5670-5689 None:intergenic 30.0%
ACATGTTGTTCACTTATTAC+AGG + tig0032745:5732-5751 Msa1449900:intron 30.0%
TATTGGTAGAACTGAAGAAA+GGG + tig0032745:5791-5810 Msa1449900:CDS 30.0%
ATTTGATTGAAGAGAATGCA+AGG + tig0032745:5842-5861 Msa1449900:CDS 30.0%
AATCTTGATTAATACCAGGT+GGG + tig0032745:6022-6041 Msa1449900:intron 30.0%
CATCACAAACATGAATCAAA+TGG + tig0032745:6070-6089 Msa1449900:CDS 30.0%
TAAATTGTAGGCAAAATGGT+TGG + tig0032745:6279-6298 Msa1449900:intron 30.0%
GTTGGAAATGTTACAATCAA+AGG + tig0032745:6297-6316 Msa1449900:CDS 30.0%
! ATGTGGCTGAAATATTTTAC+AGG - tig0032745:6326-6345 None:intergenic 30.0%
GTAATATCCTGTAGATATAC+TGG - tig0032745:6404-6423 None:intergenic 30.0%
ATTACCTTACTATACACTGA+AGG - tig0032745:6458-6477 None:intergenic 30.0%
!!! GAATGGATCTGAGTTTTTTA+TGG + tig0032745:6607-6626 Msa1449900:three_prime_UTR 30.0%
ATTATCACTGATCAATCCAG+AGG + tig0032745:5024-5043 Msa1449900:CDS 35.0%
AGAAACTGAAGATGTCAACA+TGG + tig0032745:5072-5091 Msa1449900:CDS 35.0%
GTGATTTCTCAGTTGGAAAA+TGG + tig0032745:5125-5144 Msa1449900:CDS 35.0%
! GCAGTAACTTGTCAAAAGAA+TGG + tig0032745:5202-5221 Msa1449900:CDS 35.0%
CGGATTCAGATTATGAGAAA+TGG + tig0032745:5230-5249 Msa1449900:CDS 35.0%
CAGATTATGAGAAATGGAAG+TGG + tig0032745:5236-5255 Msa1449900:CDS 35.0%
! TTTGATGCACTGAAGTATCT+TGG + tig0032745:5506-5525 Msa1449900:CDS 35.0%
TCTCTTGTATGCTTAGTTCA+AGG + tig0032745:5590-5609 Msa1449900:CDS 35.0%
TATTCCAACTGACAGGAAAA+AGG + tig0032745:5616-5635 Msa1449900:CDS 35.0%
AGACATTAAAGGACCATCAT+AGG - tig0032745:5645-5664 None:intergenic 35.0%
AGACATCAATTGAGTTCTTC+TGG + tig0032745:5761-5780 Msa1449900:CDS 35.0%
GACATCAATTGAGTTCTTCT+GGG + tig0032745:5762-5781 Msa1449900:CDS 35.0%
TTCTTCAGTTCTACCAATAG+TGG - tig0032745:5790-5809 None:intergenic 35.0%
CTATTGGTAGAACTGAAGAA+AGG + tig0032745:5790-5809 Msa1449900:CDS 35.0%
TTGAAGAGAATGCAAGGTAT+TGG + tig0032745:5848-5867 Msa1449900:CDS 35.0%
TTGCATTTGATTCAGCTCAT+TGG + tig0032745:5887-5906 Msa1449900:CDS 35.0%
CAATCTTGATTAATACCAGG+TGG + tig0032745:6021-6040 Msa1449900:intron 35.0%
TGTATTGAGTCCTTTCTCAT+AGG - tig0032745:6099-6118 None:intergenic 35.0%
ATGAGAAAGGACTCAATACA+TGG + tig0032745:6099-6118 Msa1449900:CDS 35.0%
TGAGAAAGGACTCAATACAT+GGG + tig0032745:6100-6119 Msa1449900:CDS 35.0%
! AAAGTGGGTGGATTTGAATT+TGG + tig0032745:6124-6143 Msa1449900:CDS 35.0%
! CCATACTTCTAAAAACGACT+CGG - tig0032745:6162-6181 None:intergenic 35.0%
GAGATTTCCAGTATATCTAC+AGG + tig0032745:6394-6413 Msa1449900:CDS 35.0%
ATGACTGCATTTAGAAGAGA+TGG + tig0032745:6428-6447 Msa1449900:CDS 35.0%
ACATCCTTCAGTGTATAGTA+AGG + tig0032745:6451-6470 Msa1449900:CDS 35.0%
AGGTAATAAGTGAAGAAGAG+AGG + tig0032745:6471-6490 Msa1449900:CDS 35.0%
AGCATTTCGTTCCAAATATC+AGG - tig0032745:6554-6573 None:intergenic 35.0%
GGATTGTAAGATCAGTAAGT+CGG - tig0032745:6719-6738 None:intergenic 35.0%
ATTATCACTGATCAATCCAG+AGG + tig0032745:5024-5043 Msa1449900:CDS 35.0%
AGAAACTGAAGATGTCAACA+TGG + tig0032745:5072-5091 Msa1449900:CDS 35.0%
GTGATTTCTCAGTTGGAAAA+TGG + tig0032745:5125-5144 Msa1449900:CDS 35.0%
! GCAGTAACTTGTCAAAAGAA+TGG + tig0032745:5202-5221 Msa1449900:CDS 35.0%
CGGATTCAGATTATGAGAAA+TGG + tig0032745:5230-5249 Msa1449900:CDS 35.0%
CAGATTATGAGAAATGGAAG+TGG + tig0032745:5236-5255 Msa1449900:CDS 35.0%
! TTTGATGCACTGAAGTATCT+TGG + tig0032745:5506-5525 Msa1449900:CDS 35.0%
TCTCTTGTATGCTTAGTTCA+AGG + tig0032745:5590-5609 Msa1449900:CDS 35.0%
TATTCCAACTGACAGGAAAA+AGG + tig0032745:5616-5635 Msa1449900:CDS 35.0%
AGACATTAAAGGACCATCAT+AGG - tig0032745:5645-5664 None:intergenic 35.0%
AGACATCAATTGAGTTCTTC+TGG + tig0032745:5761-5780 Msa1449900:CDS 35.0%
GACATCAATTGAGTTCTTCT+GGG + tig0032745:5762-5781 Msa1449900:CDS 35.0%
TTCTTCAGTTCTACCAATAG+TGG - tig0032745:5790-5809 None:intergenic 35.0%
CTATTGGTAGAACTGAAGAA+AGG + tig0032745:5790-5809 Msa1449900:CDS 35.0%
TTGAAGAGAATGCAAGGTAT+TGG + tig0032745:5848-5867 Msa1449900:CDS 35.0%
TTGCATTTGATTCAGCTCAT+TGG + tig0032745:5887-5906 Msa1449900:CDS 35.0%
CAATCTTGATTAATACCAGG+TGG + tig0032745:6021-6040 Msa1449900:intron 35.0%
TGTATTGAGTCCTTTCTCAT+AGG - tig0032745:6099-6118 None:intergenic 35.0%
ATGAGAAAGGACTCAATACA+TGG + tig0032745:6099-6118 Msa1449900:CDS 35.0%
TGAGAAAGGACTCAATACAT+GGG + tig0032745:6100-6119 Msa1449900:CDS 35.0%
! AAAGTGGGTGGATTTGAATT+TGG + tig0032745:6124-6143 Msa1449900:CDS 35.0%
! CCATACTTCTAAAAACGACT+CGG - tig0032745:6162-6181 None:intergenic 35.0%
GAGATTTCCAGTATATCTAC+AGG + tig0032745:6394-6413 Msa1449900:CDS 35.0%
ATGACTGCATTTAGAAGAGA+TGG + tig0032745:6428-6447 Msa1449900:CDS 35.0%
ACATCCTTCAGTGTATAGTA+AGG + tig0032745:6451-6470 Msa1449900:CDS 35.0%
AGGTAATAAGTGAAGAAGAG+AGG + tig0032745:6471-6490 Msa1449900:CDS 35.0%
AGCATTTCGTTCCAAATATC+AGG - tig0032745:6554-6573 None:intergenic 35.0%
GGATTGTAAGATCAGTAAGT+CGG - tig0032745:6719-6738 None:intergenic 35.0%
TGCAAACCCAAAGAGATTCA+TGG + tig0032745:5095-5114 Msa1449900:CDS 40.0%
GACAGTTGGAATCATAGAGA+GGG - tig0032745:5168-5187 None:intergenic 40.0%
! CTTTTGACAAGTTACTGCAG+TGG - tig0032745:5200-5219 None:intergenic 40.0%
AAATGGAAGTGGAAGCCATT+AGG + tig0032745:5247-5266 Msa1449900:CDS 40.0%
AGCATGGTTTACGTGTGTTT+GGG - tig0032745:5454-5473 None:intergenic 40.0%
! AGCTTCTCGATGACATTTTC+AGG + tig0032745:5483-5502 Msa1449900:intron 40.0%
GAGAGGAAAGAGAATGATGT+TGG + tig0032745:5535-5554 Msa1449900:CDS 40.0%
AGGAAAGAGAATGATGTTGG+TGG + tig0032745:5538-5557 Msa1449900:CDS 40.0%
GGAAAGAGAATGATGTTGGT+GGG + tig0032745:5539-5558 Msa1449900:CDS 40.0%
AGCATACAAGAGATTCCCAT+TGG - tig0032745:5583-5602 None:intergenic 40.0%
AAGGAGTTATTCCAACTGAC+AGG + tig0032745:5609-5628 Msa1449900:CDS 40.0%
AGGAAAAAGGTCACCTATGA+TGG + tig0032745:5629-5648 Msa1449900:CDS 40.0%
ATGTCTTTCCATGCTATGGT+AGG + tig0032745:5659-5678 Msa1449900:intron 40.0%
!! GAGAATGCAAGGTATTGGAA+AGG + tig0032745:5853-5872 Msa1449900:CDS 40.0%
CATTTGATTCAGCTCATTGG+TGG + tig0032745:5890-5909 Msa1449900:CDS 40.0%
TACCAGGTGGGATTATTACA+TGG + tig0032745:6034-6053 Msa1449900:intron 40.0%
TTCCATGTAATAATCCCACC+TGG - tig0032745:6039-6058 None:intergenic 40.0%
AGGTGGGATTATTACATGGA+AGG + tig0032745:6038-6057 Msa1449900:intron 40.0%
CAAATGGTTGCCTATGAGAA+AGG + tig0032745:6086-6105 Msa1449900:CDS 40.0%
ACTCAATACATGGGCAAAGT+GGG + tig0032745:6109-6128 Msa1449900:CDS 40.0%
!!! AAAAACGACTCGGTTTTTCG+AGG - tig0032745:6152-6171 None:intergenic 40.0%
!!! CCGAGTCGTTTTTAGAAGTA+TGG + tig0032745:6159-6178 Msa1449900:CDS 40.0%
AGTACGTACCTGTTATGTCT+AGG - tig0032745:6187-6206 None:intergenic 40.0%
! ACTCCTTTTAGCACTACTAC+TGG - tig0032745:6362-6381 None:intergenic 40.0%
AGTAGTAGTGCTAAAAGGAG+TGG + tig0032745:6361-6380 Msa1449900:CDS 40.0%
TGCAAACCCAAAGAGATTCA+TGG + tig0032745:5095-5114 Msa1449900:CDS 40.0%
GACAGTTGGAATCATAGAGA+GGG - tig0032745:5168-5187 None:intergenic 40.0%
! CTTTTGACAAGTTACTGCAG+TGG - tig0032745:5200-5219 None:intergenic 40.0%
AAATGGAAGTGGAAGCCATT+AGG + tig0032745:5247-5266 Msa1449900:CDS 40.0%
AGCATGGTTTACGTGTGTTT+GGG - tig0032745:5454-5473 None:intergenic 40.0%
! AGCTTCTCGATGACATTTTC+AGG + tig0032745:5483-5502 Msa1449900:intron 40.0%
GAGAGGAAAGAGAATGATGT+TGG + tig0032745:5535-5554 Msa1449900:CDS 40.0%
AGGAAAGAGAATGATGTTGG+TGG + tig0032745:5538-5557 Msa1449900:CDS 40.0%
GGAAAGAGAATGATGTTGGT+GGG + tig0032745:5539-5558 Msa1449900:CDS 40.0%
AGCATACAAGAGATTCCCAT+TGG - tig0032745:5583-5602 None:intergenic 40.0%
AAGGAGTTATTCCAACTGAC+AGG + tig0032745:5609-5628 Msa1449900:CDS 40.0%
AGGAAAAAGGTCACCTATGA+TGG + tig0032745:5629-5648 Msa1449900:CDS 40.0%
ATGTCTTTCCATGCTATGGT+AGG + tig0032745:5659-5678 Msa1449900:intron 40.0%
!! GAGAATGCAAGGTATTGGAA+AGG + tig0032745:5853-5872 Msa1449900:CDS 40.0%
CATTTGATTCAGCTCATTGG+TGG + tig0032745:5890-5909 Msa1449900:CDS 40.0%
TACCAGGTGGGATTATTACA+TGG + tig0032745:6034-6053 Msa1449900:intron 40.0%
TTCCATGTAATAATCCCACC+TGG - tig0032745:6039-6058 None:intergenic 40.0%
AGGTGGGATTATTACATGGA+AGG + tig0032745:6038-6057 Msa1449900:intron 40.0%
CAAATGGTTGCCTATGAGAA+AGG + tig0032745:6086-6105 Msa1449900:CDS 40.0%
ACTCAATACATGGGCAAAGT+GGG + tig0032745:6109-6128 Msa1449900:CDS 40.0%
!!! AAAAACGACTCGGTTTTTCG+AGG - tig0032745:6152-6171 None:intergenic 40.0%
!!! CCGAGTCGTTTTTAGAAGTA+TGG + tig0032745:6159-6178 Msa1449900:CDS 40.0%
AGTACGTACCTGTTATGTCT+AGG - tig0032745:6187-6206 None:intergenic 40.0%
! ACTCCTTTTAGCACTACTAC+TGG - tig0032745:6362-6381 None:intergenic 40.0%
AGTAGTAGTGCTAAAAGGAG+TGG + tig0032745:6361-6380 Msa1449900:CDS 40.0%
TTCTTCCAATGCAAGCCAAC+TGG - tig0032745:5056-5075 None:intergenic 45.0%
GTTGGAATCATAGAGAGGGT+AGG - tig0032745:5164-5183 None:intergenic 45.0%
GGACAGTTGGAATCATAGAG+AGG - tig0032745:5169-5188 None:intergenic 45.0%
TTGTCAAAAGAATGGACGGC+CGG + tig0032745:5210-5229 Msa1449900:CDS 45.0%
CTTGGGATGGAACAACCTAA+TGG - tig0032745:5265-5284 None:intergenic 45.0%
! CATTAGGTTGTTCCATCCCA+AGG + tig0032745:5263-5282 Msa1449900:CDS 45.0%
GTTTCATCATCACCTTGGGA+TGG - tig0032745:5278-5297 None:intergenic 45.0%
CAGCATGGTTTACGTGTGTT+TGG - tig0032745:5455-5474 None:intergenic 45.0%
! GTGACCTTTTTCCTGTCAGT+TGG - tig0032745:5623-5642 None:intergenic 45.0%
GACTCAATACATGGGCAAAG+TGG + tig0032745:6108-6127 Msa1449900:CDS 45.0%
GTATGGCACCTAGACATAAC+AGG + tig0032745:6176-6195 Msa1449900:CDS 45.0%
TTTAGCACTACTACTGGTCC+TGG - tig0032745:6356-6375 None:intergenic 45.0%
GGACCAGTAGTAGTGCTAAA+AGG + tig0032745:6356-6375 Msa1449900:CDS 45.0%
GAAGAAGAGAGGCAGAAACA+AGG + tig0032745:6482-6501 Msa1449900:CDS 45.0%
GAGAGGCAGAAACAAGGTAA+TGG + tig0032745:6488-6507 Msa1449900:CDS 45.0%
TCCAAATATCAGGCACACCA+GGG - tig0032745:6544-6563 None:intergenic 45.0%
TTCCAAATATCAGGCACACC+AGG - tig0032745:6545-6564 None:intergenic 45.0%
TTCTTCCAATGCAAGCCAAC+TGG - tig0032745:5056-5075 None:intergenic 45.0%
GTTGGAATCATAGAGAGGGT+AGG - tig0032745:5164-5183 None:intergenic 45.0%
GGACAGTTGGAATCATAGAG+AGG - tig0032745:5169-5188 None:intergenic 45.0%
TTGTCAAAAGAATGGACGGC+CGG + tig0032745:5210-5229 Msa1449900:CDS 45.0%
CTTGGGATGGAACAACCTAA+TGG - tig0032745:5265-5284 None:intergenic 45.0%
! CATTAGGTTGTTCCATCCCA+AGG + tig0032745:5263-5282 Msa1449900:CDS 45.0%
GTTTCATCATCACCTTGGGA+TGG - tig0032745:5278-5297 None:intergenic 45.0%
CAGCATGGTTTACGTGTGTT+TGG - tig0032745:5455-5474 None:intergenic 45.0%
! GTGACCTTTTTCCTGTCAGT+TGG - tig0032745:5623-5642 None:intergenic 45.0%
GACTCAATACATGGGCAAAG+TGG + tig0032745:6108-6127 Msa1449900:CDS 45.0%
GTATGGCACCTAGACATAAC+AGG + tig0032745:6176-6195 Msa1449900:CDS 45.0%
TTTAGCACTACTACTGGTCC+TGG - tig0032745:6356-6375 None:intergenic 45.0%
GGACCAGTAGTAGTGCTAAA+AGG + tig0032745:6356-6375 Msa1449900:CDS 45.0%
GAAGAAGAGAGGCAGAAACA+AGG + tig0032745:6482-6501 Msa1449900:CDS 45.0%
GAGAGGCAGAAACAAGGTAA+TGG + tig0032745:6488-6507 Msa1449900:CDS 45.0%
TCCAAATATCAGGCACACCA+GGG - tig0032745:6544-6563 None:intergenic 45.0%
TTCCAAATATCAGGCACACC+AGG - tig0032745:6545-6564 None:intergenic 45.0%
!! ATCCAGAGGAAGTGTCCAGT+TGG + tig0032745:5038-5057 Msa1449900:CDS 50.0%
!! AGTGTCCAGTTGGCTTGCAT+TGG + tig0032745:5048-5067 Msa1449900:CDS 50.0%
AGTGGCGAGATAAGGACAGT+TGG - tig0032745:5182-5201 None:intergenic 50.0%
GTTACTGCAGTGGCGAGATA+AGG - tig0032745:5190-5209 None:intergenic 50.0%
GTTCTTCTGGGCACCACTAT+TGG + tig0032745:5774-5793 Msa1449900:CDS 50.0%
!! GCTCATTGGTGGACTCATTC+TGG + tig0032745:5901-5920 Msa1449900:CDS 50.0%
CAATACATGGGCAAAGTGGG+TGG + tig0032745:6112-6131 Msa1449900:CDS 50.0%
TTCAGCCACATGCATGTTCC+AGG + tig0032745:6335-6354 Msa1449900:CDS 50.0%
AATGGCTGCAATCAGAGGAC+TGG - tig0032745:6514-6533 None:intergenic 50.0%
AGTCCTCTGATTGCAGCCAT+TGG + tig0032745:6513-6532 Msa1449900:CDS 50.0%
AAATATCAGGCACACCAGGG+AGG - tig0032745:6541-6560 None:intergenic 50.0%
TCCCTGGTGTGCCTGATATT+TGG + tig0032745:6540-6559 Msa1449900:CDS 50.0%
GGAACGAAATGCTGAGTGCA+TGG + tig0032745:6561-6580 Msa1449900:CDS 50.0%
!! ATCCAGAGGAAGTGTCCAGT+TGG + tig0032745:5038-5057 Msa1449900:CDS 50.0%
!! AGTGTCCAGTTGGCTTGCAT+TGG + tig0032745:5048-5067 Msa1449900:CDS 50.0%
AGTGGCGAGATAAGGACAGT+TGG - tig0032745:5182-5201 None:intergenic 50.0%
GTTACTGCAGTGGCGAGATA+AGG - tig0032745:5190-5209 None:intergenic 50.0%
GTTCTTCTGGGCACCACTAT+TGG + tig0032745:5774-5793 Msa1449900:CDS 50.0%
!! GCTCATTGGTGGACTCATTC+TGG + tig0032745:5901-5920 Msa1449900:CDS 50.0%
CAATACATGGGCAAAGTGGG+TGG + tig0032745:6112-6131 Msa1449900:CDS 50.0%
TTCAGCCACATGCATGTTCC+AGG + tig0032745:6335-6354 Msa1449900:CDS 50.0%
AATGGCTGCAATCAGAGGAC+TGG - tig0032745:6514-6533 None:intergenic 50.0%
AGTCCTCTGATTGCAGCCAT+TGG + tig0032745:6513-6532 Msa1449900:CDS 50.0%
AAATATCAGGCACACCAGGG+AGG - tig0032745:6541-6560 None:intergenic 50.0%
TCCCTGGTGTGCCTGATATT+TGG + tig0032745:6540-6559 Msa1449900:CDS 50.0%
GGAACGAAATGCTGAGTGCA+TGG + tig0032745:6561-6580 Msa1449900:CDS 50.0%
AGCCAACTGGACACTTCCTC+TGG - tig0032745:5043-5062 None:intergenic 55.0%
CTGGTCCTGGAACATGCATG+TGG - tig0032745:6343-6362 None:intergenic 55.0%
GCACCAATGGCTGCAATCAG+AGG - tig0032745:6519-6538 None:intergenic 55.0%
AGCCAACTGGACACTTCCTC+TGG - tig0032745:5043-5062 None:intergenic 55.0%
CTGGTCCTGGAACATGCATG+TGG - tig0032745:6343-6362 None:intergenic 55.0%
GCACCAATGGCTGCAATCAG+AGG - tig0032745:6519-6538 None:intergenic 55.0%
! TGCAGCCATTGGTGCCTCCC+TGG + tig0032745:6524-6543 Msa1449900:CDS 65.0%
!! GCACACCAGGGAGGCACCAA+TGG - tig0032745:6532-6551 None:intergenic 65.0%
! TGCAGCCATTGGTGCCTCCC+TGG + tig0032745:6524-6543 Msa1449900:CDS 65.0%
!! GCACACCAGGGAGGCACCAA+TGG - tig0032745:6532-6551 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0032745 gene 4962 6747 4962 ID=Msa1449900;Name=Msa1449900
tig0032745 mRNA 4962 6747 4962 ID=Msa1449900-mRNA-1;Parent=Msa1449900;Name=Msa1449900-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0.75|0.6|1|1|1|5|158|379
tig0032745 exon 4962 5284 4962 ID=Msa1449900-mRNA-1:exon:17181;Parent=Msa1449900-mRNA-1
tig0032745 exon 5505 5676 5505 ID=Msa1449900-mRNA-1:exon:17182;Parent=Msa1449900-mRNA-1
tig0032745 exon 5754 5935 5754 ID=Msa1449900-mRNA-1:exon:17183;Parent=Msa1449900-mRNA-1
tig0032745 exon 6040 6201 6040 ID=Msa1449900-mRNA-1:exon:17184;Parent=Msa1449900-mRNA-1
tig0032745 exon 6289 6747 6289 ID=Msa1449900-mRNA-1:exon:17185;Parent=Msa1449900-mRNA-1
tig0032745 CDS 4962 5284 4962 ID=Msa1449900-mRNA-1:cds;Parent=Msa1449900-mRNA-1
tig0032745 CDS 5505 5676 5505 ID=Msa1449900-mRNA-1:cds;Parent=Msa1449900-mRNA-1
tig0032745 CDS 5754 5935 5754 ID=Msa1449900-mRNA-1:cds;Parent=Msa1449900-mRNA-1
tig0032745 CDS 6040 6201 6040 ID=Msa1449900-mRNA-1:cds;Parent=Msa1449900-mRNA-1
tig0032745 CDS 6289 6589 6289 ID=Msa1449900-mRNA-1:cds;Parent=Msa1449900-mRNA-1
tig0032745 three_prime_UTR 6590 6747 6590 ID=Msa1449900-mRNA-1:three_prime_utr;Parent=Msa1449900-mRNA-1
Gene Sequence

>Msa1449900

ATGGCAAAAACAAACCTTCATTTACTGTCATTTTTATTCTTCTTAGGCTTATTCAACTGCACATTATCACTGATCAATCCAGAGGAAGTGTCCAGTTGGCTTGCATTGGAAGAAACTGAAGATGTCAACATGGTGCAAACCCAAAGAGATTCATGGAAGAAATGTGATTTCTCAGTTGGAAAATGGATATTTGATCAAACCTACCCTCTCTATGATTCCAACTGTCCTTATCTCGCCACTGCAGTAACTTGTCAAAAGAATGGACGGCCGGATTCAGATTATGAGAAATGGAAGTGGAAGCCATTAGGTTGTTCCATCCCAAGGTTTGATGCACTGAAGTATCTTGGTAAAATGAGAGGAAAGAGAATGATGTTGGTGGGCGATTCTATAATGAGAAACCAATGGGAATCTCTTGTATGCTTAGTTCAAGGAGTTATTCCAACTGACAGGAAAAAGGTCACCTATGATGGTCCTTTAATGTCTTTCCATGCTATGGATTTTGAGACATCAATTGAGTTCTTCTGGGCACCACTATTGGTAGAACTGAAGAAAGGGTCTAATAATAAGAGAATTCTACATTTGGATTTGATTGAAGAGAATGCAAGGTATTGGAAAGGAGTTGATATTCTTGCATTTGATTCAGCTCATTGGTGGACTCATTCTGGTCAATCTAGCTCGTGGGATTATTACATGGAAGGAAATAGTATCATCACAAACATGAATCAAATGGTTGCCTATGAGAAAGGACTCAATACATGGGCAAAGTGGGTGGATTTGAATTTGGATCCTCGAAAAACCGAGTCGTTTTTAGAAGTATGGCACCTAGACATAACAGGTACGCAAAATGGTTGGAAATGTTACAATCAAAGGCAACCTGTAAAATATTTCAGCCACATGCATGTTCCAGGACCAGTAGTAGTGCTAAAAGGAGTGGTGAAGAGAATGAGATTTCCAGTATATCTACAGGATATTACAACAATGACTGCATTTAGAAGAGATGGACATCCTTCAGTGTATAGTAAGGTAATAAGTGAAGAAGAGAGGCAGAAACAAGGTAATGGCCAGTCCTCTGATTGCAGCCATTGGTGCCTCCCTGGTGTGCCTGATATTTGGAACGAAATGCTGAGTGCATGGCTTTAA
Protein sequence

>Msa1449900

MAKTNLHLLSFLFFLGLFNCTLSLINPEEVSSWLALEETEDVNMVQTQRDSWKKCDFSVGKWIFDQTYPLYDSNCPYLATAVTCQKNGRPDSDYEKWKWKPLGCSIPRFDALKYLGKMRGKRMMLVGDSIMRNQWESLVCLVQGVIPTDRKKVTYDGPLMSFHAMDFETSIEFFWAPLLVELKKGSNNKRILHLDLIEENARYWKGVDILAFDSAHWWTHSGQSSSWDYYMEGNSIITNMNQMVAYEKGLNTWAKWVDLNLDPRKTESFLEVWHLDITGTQNGWKCYNQRQPVKYFSHMHVPGPVVVLKGVVKRMRFPVYLQDITTMTAFRRDGHPSVYSKVISEEERQKQGNGQSSDCSHWCLPGVPDIWNEMLSAWL*