AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0966750


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0966750 MtrunA17_Chr6g0487951 87.879 33 4 0 1 33 1 33 5.41e-15 62.4
Msa0966750 MtrunA17_Chr8g0389431 81.818 33 6 0 1 33 1 33 3.60e-13 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 22 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGATCTCCTTGATGGCTTT+TGG 0.181319 6_3:-95446720 None:intergenic
AAACTTTCTGATCTCCTTGA+TGG 0.303540 6_3:-95446727 None:intergenic
ACCAGCCATGGAGACGATTA+TGG 0.347553 6_3:-95445637 None:intergenic
TTAATCTCAGAAGCTTAAAA+TGG 0.362156 6_3:+95445553 Msa0966750:intron
TCCATAATCGTCTCCATGGC+TGG 0.466803 6_3:+95445636 Msa0966750:CDS
CGAAATAGCCTTGATCAGCT+TGG 0.492465 6_3:-95446790 None:intergenic
GAGACGATTATGGAGATTGA+TGG 0.492577 6_3:-95445627 None:intergenic
TTACAGTACTTACAAGAAGT+AGG 0.501858 6_3:+95446695 Msa0966750:intron
AAGATTGAAATTACCAGCCA+TGG 0.508931 6_3:-95445649 None:intergenic
TCTCAGAAGCTTAAAATGGT+GGG 0.524841 6_3:+95445557 Msa0966750:intron
GTAGGCACCAAAAGCCATCA+AGG 0.525139 6_3:+95446713 Msa0966750:exon
TCTAACATCGTTAGTGCCCA+TGG 0.538776 6_3:-95446760 None:intergenic
GAAGCTTAAAATGGTGGGGA+AGG 0.552835 6_3:+95445562 Msa0966750:intron
ATCTCAGAAGCTTAAAATGG+TGG 0.554033 6_3:+95445556 Msa0966750:intron
AGTTGATGCCAAGCTGATCA+AGG 0.554096 6_3:+95446782 Msa0966750:three_prime_UTR
CTCAGAAGCTTAAAATGGTG+GGG 0.563857 6_3:+95445558 Msa0966750:intron
AATCTCCATAATCGTCTCCA+TGG 0.567088 6_3:+95445632 Msa0966750:CDS
TGCAGCACGTAGAAAAGAAG+AGG 0.581022 6_3:+95445586 Msa0966750:CDS
AAAAGAAGAGGTGGTTACAC+GGG 0.584738 6_3:+95445598 Msa0966750:CDS
GAAAAGAAGAGGTGGTTACA+CGG 0.586191 6_3:+95445597 Msa0966750:CDS
AAGCTTAAAATGGTGGGGAA+GGG 0.609608 6_3:+95445563 Msa0966750:exon
AGCACGTAGAAAAGAAGAGG+TGG 0.682543 6_3:+95445589 Msa0966750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTAAAATCAAAATCAT+GGG - chr6_3:95445750-95445769 None:intergenic 10.0%
!! TTTAATTAAAATCAAAATCA+TGG - chr6_3:95445751-95445770 None:intergenic 10.0%
!! TTAATTAAAATCAAAATCAT+GGG - chr6_3:95445750-95445769 None:intergenic 10.0%
!! TTTAATTAAAATCAAAATCA+TGG - chr6_3:95445751-95445770 None:intergenic 10.0%
!! TTAATTAAAATCAAAATCAT+GGG - chr6_3:95445750-95445769 None:intergenic 10.0%
!! TTTAATTAAAATCAAAATCA+TGG - chr6_3:95445751-95445770 None:intergenic 10.0%
!! TTAATTAAAATCAAAATCAT+GGG - chr6_3:95445750-95445769 None:intergenic 10.0%
!! TTTAATTAAAATCAAAATCA+TGG - chr6_3:95445751-95445770 None:intergenic 10.0%
!! GAAATGTTTCATTGAAAATT+TGG + chr6_3:95445871-95445890 Msa0966750:intron 20.0%
!! CTAAGTTATTTATCAATACA+GGG - chr6_3:95446001-95446020 None:intergenic 20.0%
!!! CATATGCTATTTTTCATAAA+AGG - chr6_3:95446200-95446219 None:intergenic 20.0%
!! TGAAAACATATTTGCATTAA+AGG - chr6_3:95446234-95446253 None:intergenic 20.0%
!! TTACAAATAATCAAAGAACT+AGG - chr6_3:95446388-95446407 None:intergenic 20.0%
!!! TAAGCTATTTTTATTGCAAA+AGG + chr6_3:95446450-95446469 Msa0966750:intron 20.0%
!! TGTTTGTGTAATTGTATATT+TGG + chr6_3:95446620-95446639 Msa0966750:intron 20.0%
!! AGTTTCTGTAATTGTTTAAA+TGG + chr6_3:95446653-95446672 Msa0966750:intron 20.0%
!! GAAATGTTTCATTGAAAATT+TGG + chr6_3:95445871-95445890 Msa0966750:intron 20.0%
!! CTAAGTTATTTATCAATACA+GGG - chr6_3:95446001-95446020 None:intergenic 20.0%
!!! CATATGCTATTTTTCATAAA+AGG - chr6_3:95446200-95446219 None:intergenic 20.0%
!! TGAAAACATATTTGCATTAA+AGG - chr6_3:95446234-95446253 None:intergenic 20.0%
!! TTACAAATAATCAAAGAACT+AGG - chr6_3:95446388-95446407 None:intergenic 20.0%
!!! TAAGCTATTTTTATTGCAAA+AGG + chr6_3:95446450-95446469 Msa0966750:intron 20.0%
!! TGTTTGTGTAATTGTATATT+TGG + chr6_3:95446620-95446639 Msa0966750:intron 20.0%
!! AGTTTCTGTAATTGTTTAAA+TGG + chr6_3:95446653-95446672 Msa0966750:intron 20.0%
! TTAATCTCAGAAGCTTAAAA+TGG + chr6_3:95445553-95445572 Msa0966750:intron 25.0%
! AAGCCATATTTATCAATACA+GGG - chr6_3:95445795-95445814 None:intergenic 25.0%
! TTTCAATAAAACACAGAGAA+TGG - chr6_3:95445855-95445874 None:intergenic 25.0%
! GCTAAGTTATTTATCAATAC+AGG - chr6_3:95446002-95446021 None:intergenic 25.0%
! AAAAAACAGCCTATTTAAAC+AGG - chr6_3:95446158-95446177 None:intergenic 25.0%
!! TGCAAATATGTTTTCACTAA+CGG + chr6_3:95446238-95446257 Msa0966750:intron 25.0%
! AAGATAAGTCTAATCAAAAC+TGG + chr6_3:95446361-95446380 Msa0966750:intron 25.0%
!!! TGAGAAGCACTTATTAAAAA+CGG - chr6_3:95446486-95446505 None:intergenic 25.0%
! ATACAATTACACAAACATCA+AGG - chr6_3:95446617-95446636 None:intergenic 25.0%
! AAACAATTACAGAAACTACA+AGG - chr6_3:95446650-95446669 None:intergenic 25.0%
!! GAATTGTAATTTTGTTAGAC+TGG + chr6_3:95446844-95446863 Msa0966750:three_prime_UTR 25.0%
! TTAATCTCAGAAGCTTAAAA+TGG + chr6_3:95445553-95445572 Msa0966750:intron 25.0%
! AAGCCATATTTATCAATACA+GGG - chr6_3:95445795-95445814 None:intergenic 25.0%
! TTTCAATAAAACACAGAGAA+TGG - chr6_3:95445855-95445874 None:intergenic 25.0%
! GCTAAGTTATTTATCAATAC+AGG - chr6_3:95446002-95446021 None:intergenic 25.0%
! AAAAAACAGCCTATTTAAAC+AGG - chr6_3:95446158-95446177 None:intergenic 25.0%
!! TGCAAATATGTTTTCACTAA+CGG + chr6_3:95446238-95446257 Msa0966750:intron 25.0%
! AAGATAAGTCTAATCAAAAC+TGG + chr6_3:95446361-95446380 Msa0966750:intron 25.0%
!!! TGAGAAGCACTTATTAAAAA+CGG - chr6_3:95446486-95446505 None:intergenic 25.0%
! ATACAATTACACAAACATCA+AGG - chr6_3:95446617-95446636 None:intergenic 25.0%
! AAACAATTACAGAAACTACA+AGG - chr6_3:95446650-95446669 None:intergenic 25.0%
TTTCTATCATTTGCTTCTCA+TGG + chr6_3:95445670-95445689 Msa0966750:intron 30.0%
! AGACCCTGTATTGATAAATA+TGG + chr6_3:95445789-95445808 Msa0966750:intron 30.0%
CAAGCCATATTTATCAATAC+AGG - chr6_3:95445796-95445815 None:intergenic 30.0%
TATTTATCAATACAGGGTCT+AGG - chr6_3:95445789-95445808 None:intergenic 30.0%
!!! TAAGCTGTTTTTATTCGCTA+AGG + chr6_3:95446125-95446144 Msa0966750:intron 30.0%
! GCAAATATGTTTTCACTAAC+GGG + chr6_3:95446239-95446258 Msa0966750:intron 30.0%
TTAGACTTATCTTGAGATCA+TGG - chr6_3:95446354-95446373 None:intergenic 30.0%
TGTTTAAATGAAAGCAGCTT+AGG + chr6_3:95446514-95446533 Msa0966750:intron 30.0%
! TCATTTAATTGCTGGCTATT+TGG - chr6_3:95446572-95446591 None:intergenic 30.0%
ACTGTGTTTCATTTAATTGC+TGG - chr6_3:95446580-95446599 None:intergenic 30.0%
TTACAGTACTTACAAGAAGT+AGG + chr6_3:95446695-95446714 Msa0966750:intron 30.0%
TTTCTATCATTTGCTTCTCA+TGG + chr6_3:95445670-95445689 Msa0966750:intron 30.0%
! AGACCCTGTATTGATAAATA+TGG + chr6_3:95445789-95445808 Msa0966750:intron 30.0%
CAAGCCATATTTATCAATAC+AGG - chr6_3:95445796-95445815 None:intergenic 30.0%
TATTTATCAATACAGGGTCT+AGG - chr6_3:95445789-95445808 None:intergenic 30.0%
!!! TAAGCTGTTTTTATTCGCTA+AGG + chr6_3:95446125-95446144 Msa0966750:intron 30.0%
! GCAAATATGTTTTCACTAAC+GGG + chr6_3:95446239-95446258 Msa0966750:intron 30.0%
TTAGACTTATCTTGAGATCA+TGG - chr6_3:95446354-95446373 None:intergenic 30.0%
TGTTTAAATGAAAGCAGCTT+AGG + chr6_3:95446514-95446533 Msa0966750:intron 30.0%
! TCATTTAATTGCTGGCTATT+TGG - chr6_3:95446572-95446591 None:intergenic 30.0%
ACTGTGTTTCATTTAATTGC+TGG - chr6_3:95446580-95446599 None:intergenic 30.0%
TTACAGTACTTACAAGAAGT+AGG + chr6_3:95446695-95446714 Msa0966750:intron 30.0%
ATCTCAGAAGCTTAAAATGG+TGG + chr6_3:95445556-95445575 Msa0966750:intron 35.0%
TCTCAGAAGCTTAAAATGGT+GGG + chr6_3:95445557-95445576 Msa0966750:intron 35.0%
AAGATTGAAATTACCAGCCA+TGG - chr6_3:95445652-95445671 None:intergenic 35.0%
AAACTTTCTGATCTCCTTGA+TGG - chr6_3:95446730-95446749 None:intergenic 35.0%
AAAGTTTGCACAAAAAGCCA+TGG + chr6_3:95446743-95446762 Msa0966750:three_prime_UTR 35.0%
AAGTTTGCACAAAAAGCCAT+GGG + chr6_3:95446744-95446763 Msa0966750:three_prime_UTR 35.0%
ATCTCAGAAGCTTAAAATGG+TGG + chr6_3:95445556-95445575 Msa0966750:intron 35.0%
TCTCAGAAGCTTAAAATGGT+GGG + chr6_3:95445557-95445576 Msa0966750:intron 35.0%
AAGATTGAAATTACCAGCCA+TGG - chr6_3:95445652-95445671 None:intergenic 35.0%
AAACTTTCTGATCTCCTTGA+TGG - chr6_3:95446730-95446749 None:intergenic 35.0%
AAAGTTTGCACAAAAAGCCA+TGG + chr6_3:95446743-95446762 Msa0966750:three_prime_UTR 35.0%
AAGTTTGCACAAAAAGCCAT+GGG + chr6_3:95446744-95446763 Msa0966750:three_prime_UTR 35.0%
CTCAGAAGCTTAAAATGGTG+GGG + chr6_3:95445558-95445577 Msa0966750:intron 40.0%
! AAGCTTAAAATGGTGGGGAA+GGG + chr6_3:95445563-95445582 Msa0966750:exon 40.0%
GAAAAGAAGAGGTGGTTACA+CGG + chr6_3:95445597-95445616 Msa0966750:CDS 40.0%
AAAAGAAGAGGTGGTTACAC+GGG + chr6_3:95445598-95445617 Msa0966750:CDS 40.0%
GAGACGATTATGGAGATTGA+TGG - chr6_3:95445630-95445649 None:intergenic 40.0%
AATCTCCATAATCGTCTCCA+TGG + chr6_3:95445632-95445651 Msa0966750:CDS 40.0%
! GTTTTTATTCGCTAAGGACC+TGG + chr6_3:95446131-95446150 Msa0966750:intron 40.0%
CTCAGAAGCTTAAAATGGTG+GGG + chr6_3:95445558-95445577 Msa0966750:intron 40.0%
! AAGCTTAAAATGGTGGGGAA+GGG + chr6_3:95445563-95445582 Msa0966750:exon 40.0%
GAAAAGAAGAGGTGGTTACA+CGG + chr6_3:95445597-95445616 Msa0966750:CDS 40.0%
AAAAGAAGAGGTGGTTACAC+GGG + chr6_3:95445598-95445617 Msa0966750:CDS 40.0%
GAGACGATTATGGAGATTGA+TGG - chr6_3:95445630-95445649 None:intergenic 40.0%
AATCTCCATAATCGTCTCCA+TGG + chr6_3:95445632-95445651 Msa0966750:CDS 40.0%
! GTTTTTATTCGCTAAGGACC+TGG + chr6_3:95446131-95446150 Msa0966750:intron 40.0%
! GAAGCTTAAAATGGTGGGGA+AGG + chr6_3:95445562-95445581 Msa0966750:intron 45.0%
TGCAGCACGTAGAAAAGAAG+AGG + chr6_3:95445586-95445605 Msa0966750:CDS 45.0%
AGCACGTAGAAAAGAAGAGG+TGG + chr6_3:95445589-95445608 Msa0966750:CDS 45.0%
GGACCTGGACCTGTTTAAAT+AGG + chr6_3:95446146-95446165 Msa0966750:intron 45.0%
CAGCCTATTTAAACAGGTCC+AGG - chr6_3:95446152-95446171 None:intergenic 45.0%
!! CTGATCTCCTTGATGGCTTT+TGG - chr6_3:95446723-95446742 None:intergenic 45.0%
TCTAACATCGTTAGTGCCCA+TGG - chr6_3:95446763-95446782 None:intergenic 45.0%
! AGTTGATGCCAAGCTGATCA+AGG + chr6_3:95446782-95446801 Msa0966750:three_prime_UTR 45.0%
! CGAAATAGCCTTGATCAGCT+TGG - chr6_3:95446793-95446812 None:intergenic 45.0%
! GAAGCTTAAAATGGTGGGGA+AGG + chr6_3:95445562-95445581 Msa0966750:intron 45.0%
TGCAGCACGTAGAAAAGAAG+AGG + chr6_3:95445586-95445605 Msa0966750:CDS 45.0%
AGCACGTAGAAAAGAAGAGG+TGG + chr6_3:95445589-95445608 Msa0966750:CDS 45.0%
GGACCTGGACCTGTTTAAAT+AGG + chr6_3:95446146-95446165 Msa0966750:intron 45.0%
CAGCCTATTTAAACAGGTCC+AGG - chr6_3:95446152-95446171 None:intergenic 45.0%
!! CTGATCTCCTTGATGGCTTT+TGG - chr6_3:95446723-95446742 None:intergenic 45.0%
TCTAACATCGTTAGTGCCCA+TGG - chr6_3:95446763-95446782 None:intergenic 45.0%
! AGTTGATGCCAAGCTGATCA+AGG + chr6_3:95446782-95446801 Msa0966750:three_prime_UTR 45.0%
! CGAAATAGCCTTGATCAGCT+TGG - chr6_3:95446793-95446812 None:intergenic 45.0%
TCCATAATCGTCTCCATGGC+TGG + chr6_3:95445636-95445655 Msa0966750:CDS 50.0%
ACCAGCCATGGAGACGATTA+TGG - chr6_3:95445640-95445659 None:intergenic 50.0%
GTAGGCACCAAAAGCCATCA+AGG + chr6_3:95446713-95446732 Msa0966750:exon 50.0%
TCCATAATCGTCTCCATGGC+TGG + chr6_3:95445636-95445655 Msa0966750:CDS 50.0%
ACCAGCCATGGAGACGATTA+TGG - chr6_3:95445640-95445659 None:intergenic 50.0%
GTAGGCACCAAAAGCCATCA+AGG + chr6_3:95446713-95446732 Msa0966750:exon 50.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 95445545 95446919 95445545 ID=Msa0966750;Name=Msa0966750
chr6_3 mRNA 95445545 95446919 95445545 ID=Msa0966750-mRNA-1;Parent=Msa0966750;Name=Msa0966750-mRNA-1;_AED=0.12;_eAED=0.12;_QI=16|0.5|0.33|1|0|0|3|203|33
chr6_3 exon 95445545 95445551 95445545 ID=Msa0966750-mRNA-1:exon:17674;Parent=Msa0966750-mRNA-1
chr6_3 exon 95445563 95445657 95445563 ID=Msa0966750-mRNA-1:exon:17675;Parent=Msa0966750-mRNA-1
chr6_3 exon 95446701 95446919 95446701 ID=Msa0966750-mRNA-1:exon:17676;Parent=Msa0966750-mRNA-1
chr6_3 five_prime_UTR 95445545 95445551 95445545 ID=Msa0966750-mRNA-1:five_prime_utr;Parent=Msa0966750-mRNA-1
chr6_3 five_prime_UTR 95445563 95445571 95445563 ID=Msa0966750-mRNA-1:five_prime_utr;Parent=Msa0966750-mRNA-1
chr6_3 CDS 95445572 95445657 95445572 ID=Msa0966750-mRNA-1:cds;Parent=Msa0966750-mRNA-1
chr6_3 CDS 95446701 95446716 95446701 ID=Msa0966750-mRNA-1:cds;Parent=Msa0966750-mRNA-1
chr6_3 three_prime_UTR 95446717 95446919 95446717 ID=Msa0966750-mRNA-1:three_prime_utr;Parent=Msa0966750-mRNA-1
Gene Sequence

>Msa0966750

ATGGTGGGGAAGGGTGCAGCACGTAGAAAAGAAGAGGTGGTTACACGGGAGTATACCATCAATCTCCATAATCGTCTCCATGGCTGTACTTACAAGAAGTAG

Protein sequence

>Msa0966750

MVGKGAARRKEEVVTREYTINLHNRLHGCTYKK*