AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0899430


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0899430 MtrunA17_Chr6g0485551 75.591 127 13 1 1 109 70 196 2.78e-14 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGCCTTCGAAGTTCTTTA+CGG 0.344809 6_1:-94509113 None:intergenic
AAGTTGAAATTCTGGAAATA+AGG 0.355807 6_1:+94509157 Msa0899430:CDS
AGGCTGAAAACTGCGTGGTT+TGG 0.421494 6_1:+94509057 Msa0899430:CDS
GGAAGCTGAAAGGAAGGAAA+AGG 0.444058 6_1:+94509221 Msa0899430:CDS
AGAGGAGGAAGCTGAAAGGA+AGG 0.454150 6_1:+94509215 Msa0899430:CDS
CTCTAAACCAGCAGTTGTTT+AGG 0.457152 6_1:+94509037 None:intergenic
AGGAGGAAGCTGAAATGAAA+AGG 0.473270 6_1:+94509187 Msa0899430:CDS
GGAAGCTGAAATGAAAAGGA+AGG 0.493440 6_1:+94509191 Msa0899430:CDS
TCTGGAAATAAGGAAGGAAA+AGG 0.493692 6_1:+94509167 Msa0899430:CDS
AGCTGAAAGGAAGGAAAAGG+AGG 0.497172 6_1:+94509224 Msa0899430:CDS
AGGAAGAGGAGGAAGCTGAA+AGG 0.516462 6_1:+94509211 Msa0899430:CDS
TGAAATTCTGGAAATAAGGA+AGG 0.531101 6_1:+94509161 Msa0899430:CDS
TGAAATGAAAAGGAAGGAAG+AGG 0.532757 6_1:+94509197 Msa0899430:CDS
GGAAATAAGGAAGGAAAAGG+AGG 0.550433 6_1:+94509170 Msa0899430:CDS
AAAACTGCGTGGTTTGGCAA+AGG 0.574300 6_1:+94509063 Msa0899430:CDS
AATGAAAAGGAAGGAAGAGG+AGG 0.596938 6_1:+94509200 Msa0899430:CDS
AAAACCGTAAAGAACTTCGA+AGG 0.658384 6_1:+94509109 Msa0899430:CDS
TGTTTAGGCTGAAAACTGCG+TGG 0.725223 6_1:+94509052 Msa0899430:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAGCAAATTAAAAAAA+AGG + chr6_1:94509134-94509153 Msa0899430:CDS 15.0%
!! GAAAAAGAAAATGAAAAAGA+AGG + chr6_1:94509296-94509315 Msa0899430:CDS 20.0%
! AAAAAAGGAAGTTGAAATTC+TGG + chr6_1:94509149-94509168 Msa0899430:CDS 25.0%
!! AAGTTGAAATTCTGGAAATA+AGG + chr6_1:94509157-94509176 Msa0899430:CDS 25.0%
!! TGAAATTCTGAAAAAAAGCA+AGG + chr6_1:94509251-94509270 Msa0899430:CDS 25.0%
! TGAAATTCTGGAAATAAGGA+AGG + chr6_1:94509161-94509180 Msa0899430:CDS 30.0%
GAAAAAAAGCAAGGAAAAGA+AGG + chr6_1:94509260-94509279 Msa0899430:CDS 30.0%
CTAAAAAAGTTTGTGAGGAA+GGG + chr6_1:94509343-94509362 Msa0899430:CDS 30.0%
TAAAAAAGTTTGTGAGGAAG+GGG + chr6_1:94509344-94509363 Msa0899430:CDS 30.0%
! TCGAAGTTCTTTACGGTTTT+CGG - chr6_1:94509109-94509128 None:intergenic 35.0%
AAAACCGTAAAGAACTTCGA+AGG + chr6_1:94509109-94509128 Msa0899430:CDS 35.0%
TCTGGAAATAAGGAAGGAAA+AGG + chr6_1:94509167-94509186 Msa0899430:CDS 35.0%
TGAAATGAAAAGGAAGGAAG+AGG + chr6_1:94509197-94509216 Msa0899430:CDS 35.0%
AGCAGCTAAAAAAGTTTGTG+AGG + chr6_1:94509338-94509357 Msa0899430:CDS 35.0%
GCTAAAAAAGTTTGTGAGGA+AGG + chr6_1:94509342-94509361 Msa0899430:CDS 35.0%
TCAGCCTTCGAAGTTCTTTA+CGG - chr6_1:94509116-94509135 None:intergenic 40.0%
GGAAATAAGGAAGGAAAAGG+AGG + chr6_1:94509170-94509189 Msa0899430:CDS 40.0%
AGGAGGAAGCTGAAATGAAA+AGG + chr6_1:94509187-94509206 Msa0899430:CDS 40.0%
GGAAGCTGAAATGAAAAGGA+AGG + chr6_1:94509191-94509210 Msa0899430:CDS 40.0%
AATGAAAAGGAAGGAAGAGG+AGG + chr6_1:94509200-94509219 Msa0899430:CDS 40.0%
TGTTTAGGCTGAAAACTGCG+TGG + chr6_1:94509052-94509071 Msa0899430:CDS 45.0%
! AAAACTGCGTGGTTTGGCAA+AGG + chr6_1:94509063-94509082 Msa0899430:CDS 45.0%
GGAAGCTGAAAGGAAGGAAA+AGG + chr6_1:94509221-94509240 Msa0899430:CDS 45.0%
AGCTGAAAGGAAGGAAAAGG+AGG + chr6_1:94509224-94509243 Msa0899430:CDS 45.0%
AGGCTGAAAACTGCGTGGTT+TGG + chr6_1:94509057-94509076 Msa0899430:CDS 50.0%
AGGAAGAGGAGGAAGCTGAA+AGG + chr6_1:94509211-94509230 Msa0899430:CDS 50.0%
AGAGGAGGAAGCTGAAAGGA+AGG + chr6_1:94509215-94509234 Msa0899430:CDS 50.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 94509051 94509380 94509051 ID=Msa0899430;Name=Msa0899430
chr6_1 mRNA 94509051 94509380 94509051 ID=Msa0899430-mRNA-1;Parent=Msa0899430;Name=Msa0899430-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|109
chr6_1 exon 94509051 94509380 94509051 ID=Msa0899430-mRNA-1:exon:16140;Parent=Msa0899430-mRNA-1
chr6_1 CDS 94509051 94509380 94509051 ID=Msa0899430-mRNA-1:cds;Parent=Msa0899430-mRNA-1
Gene Sequence

>Msa0899430

TTGTTTAGGCTGAAAACTGCGTGGTTTGGCAAAGGTCATTATAGAGATTATGTTGCCGAAAACCGTAAAGAACTTCGAAGGCTGAAAAAGCAAATTAAAAAAAAGGAAGTTGAAATTCTGGAAATAAGGAAGGAAAAGGAGGAAGCTGAAATGAAAAGGAAGGAAGAGGAGGAAGCTGAAAGGAAGGAAAAGGAGGAAGCTGAAATTCTGAAAAAAAGCAAGGAAAAGAAGGAAGCTAAAATTCAGAAAAAGAAAATGAAAAAGAAGGAAGCTAAAATTCGAAAGCAAGCAGCTAAAAAAGTTTGTGAGGAAGGGGCTAAAGCATGTTAA

Protein sequence

>Msa0899430

LFRLKTAWFGKGHYRDYVAENRKELRRLKKQIKKKEVEILEIRKEKEEAEMKRKEEEEAERKEKEEAEILKKSKEKKEAKIQKKKMKKKEAKIRKQAAKKVCEEGAKAC*