AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1007150


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1007150 MtrunA17_Chr7g0218621 96.739 92 3 0 3 94 2 93 2.08e-60 179
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1007150 AT5G61220.3 47.561 82 43 0 8 89 3 84 2.69e-24 88.2
Msa1007150 AT5G61220.1 47.561 82 43 0 8 89 3 84 2.69e-24 88.2
Msa1007150 AT5G61220.2 47.561 82 43 0 8 89 3 84 2.69e-24 88.2

Find 21 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGATTAAAAGGGTTTATT+AGG 0.211621 7_1:+6539331 None:intergenic
GCGGAGAAGGGTTAGGGTTT+GGG 0.235231 7_1:+6539570 None:intergenic
AGCGGAGAAGGGTTAGGGTT+TGG 0.288293 7_1:+6539569 None:intergenic
TGAAGGAGCGGAGAAGGGTT+AGG 0.361491 7_1:+6539563 None:intergenic
ATTTCCGAAGCTTTCTCGTT+TGG 0.370472 7_1:-6539436 Msa1007150:CDS
TCCTTCTCTTCCAAGTGTTA+TGG 0.372181 7_1:-6539359 Msa1007150:CDS
GAAGGAGCGGAGAAGGGTTA+GGG 0.385642 7_1:+6539564 None:intergenic
TACTCGCGAATGTTGTAATC+TGG 0.389094 7_1:+6539520 None:intergenic
AAAACGAACCTTGATTAAAA+GGG 0.427832 7_1:+6539322 None:intergenic
CGCGGATGAAGGAGCGGAGA+AGG 0.441206 7_1:+6539557 None:intergenic
TTGTCGAGAAACGCGGATGA+AGG 0.469164 7_1:+6539546 None:intergenic
TCCATAACACTTGGAAGAGA+AGG 0.524453 7_1:+6539358 None:intergenic
GTCGACGACTATGGCGGCGG+CGG 0.544766 7_1:-6539608 None:intergenic
ACTCGCGAATGTTGTAATCT+GGG 0.547868 7_1:+6539521 None:intergenic
TTAGGAAGATCCATAACACT+TGG 0.559900 7_1:+6539349 None:intergenic
CCCGTCGACGACTATGGCGG+CGG 0.566090 7_1:-6539611 None:intergenic
CTGGGAATTGTCGAGAAACG+CGG 0.587534 7_1:+6539539 None:intergenic
GCGGATGAAGGAGCGGAGAA+GGG 0.605292 7_1:+6539558 None:intergenic
AAAGCTTCGGAAATCGAACA+AGG 0.638529 7_1:+6539445 None:intergenic
GAGAAACGCGGATGAAGGAG+CGG 0.654176 7_1:+6539551 None:intergenic
AGCATAAATAGAGTAAACAA+CGG 0.670929 7_1:+6539381 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGCATAAATAGAGTAAACAA+CGG + chr7_1:6539556-6539575 None:intergenic 25.0%
! TTTTCCAAACGAGAAAGCTT+CGG + chr7_1:6539505-6539524 None:intergenic 35.0%
TTAGGAAGATCCATAACACT+TGG + chr7_1:6539588-6539607 None:intergenic 35.0%
!! GTTAGGGTTTGGGATTTTGA+AGG + chr7_1:6539357-6539376 None:intergenic 40.0%
ACTCGCGAATGTTGTAATCT+GGG + chr7_1:6539416-6539435 None:intergenic 40.0%
TACTCGCGAATGTTGTAATC+TGG + chr7_1:6539417-6539436 None:intergenic 40.0%
AAAGCTTCGGAAATCGAACA+AGG + chr7_1:6539492-6539511 None:intergenic 40.0%
ATTTCCGAAGCTTTCTCGTT+TGG - chr7_1:6539498-6539517 Msa1007150:CDS 40.0%
TCCTTCTCTTCCAAGTGTTA+TGG - chr7_1:6539575-6539594 Msa1007150:CDS 40.0%
TCCATAACACTTGGAAGAGA+AGG + chr7_1:6539579-6539598 None:intergenic 40.0%
! GTTTTGTCGAAACGCATCGA+TGG + chr7_1:6539454-6539473 None:intergenic 45.0%
TTGTCGAGAAACGCGGATGA+AGG + chr7_1:6539391-6539410 None:intergenic 50.0%
CTGGGAATTGTCGAGAAACG+CGG + chr7_1:6539398-6539417 None:intergenic 50.0%
GCGGAGAAGGGTTAGGGTTT+GGG + chr7_1:6539367-6539386 None:intergenic 55.0%
AGCGGAGAAGGGTTAGGGTT+TGG + chr7_1:6539368-6539387 None:intergenic 55.0%
GAAGGAGCGGAGAAGGGTTA+GGG + chr7_1:6539373-6539392 None:intergenic 55.0%
TGAAGGAGCGGAGAAGGGTT+AGG + chr7_1:6539374-6539393 None:intergenic 55.0%
GAGAAACGCGGATGAAGGAG+CGG + chr7_1:6539386-6539405 None:intergenic 55.0%
GCGGATGAAGGAGCGGAGAA+GGG + chr7_1:6539379-6539398 None:intergenic 60.0%
CGCGGATGAAGGAGCGGAGA+AGG + chr7_1:6539380-6539399 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 6539336 6539620 6539336 ID=Msa1007150;Name=Msa1007150
chr7_1 mRNA 6539336 6539620 6539336 ID=Msa1007150-mRNA-1;Parent=Msa1007150;Name=Msa1007150-mRNA-1;_AED=0.32;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|94
chr7_1 exon 6539336 6539620 6539336 ID=Msa1007150-mRNA-1:exon:1947;Parent=Msa1007150-mRNA-1
chr7_1 CDS 6539336 6539620 6539336 ID=Msa1007150-mRNA-1:cds;Parent=Msa1007150-mRNA-1
Gene Sequence

>Msa1007150

ATGGCGGCGGCGGCGACACCTTCAAAATCCCAAACCCTAACCCTTCTCCGCTCCTTCATCCGCGTTTCTCGACAATTCCCAGATTACAACATTCGCGAGTACACAAAACGACGCGCCATCGATGCGTTTCGACAAAACGCAACACTTTCTGAACCTTGTTCGATTTCCGAAGCTTTCTCGTTTGGAAAATCTCAGCTTGATGTTGCTAAACGACAAGCCGTTGTTTACTCTATTTATGCTCCTTCTCTTCCAAGTGTTATGGATCTTCCTAATAAACCCTTTTAA

Protein sequence

>Msa1007150

MAAAATPSKSQTLTLLRSFIRVSRQFPDYNIREYTKRRAIDAFRQNATLSEPCSISEAFSFGKSQLDVAKRQAVVYSIYAPSLPSVMDLPNKPF*