Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1015950 | GAU23634.1 | 64.881 | 168 | 46 | 4 | 23 | 188 | 45 | 201 | 4.93e-61 | 210 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1015950 | A0A2Z6LVI3 | 64.881 | 168 | 46 | 4 | 23 | 188 | 45 | 201 | 2.36e-61 | 210 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0694370 | Msa1015950 | 0.802613 | 5.163437e-49 | -8.615850e-47 |
| Msa0726140 | Msa1015950 | 0.812577 | 3.953453e-51 | -8.615850e-47 |
| Msa0729800 | Msa1015950 | 0.808418 | 3.126990e-50 | -8.615850e-47 |
| Msa0808020 | Msa1015950 | 0.800199 | 1.611855e-48 | -8.615850e-47 |
| Msa0384800 | Msa1015950 | 0.801289 | 9.657467e-49 | -8.615850e-47 |
| Msa0479340 | Msa1015950 | 0.804698 | 1.906872e-49 | -8.615850e-47 |
| Msa1015950 | Msa1025370 | 0.802020 | 6.839434e-49 | -8.615850e-47 |
| Msa1015950 | Msa1100980 | 0.811586 | 6.501546e-51 | -8.615850e-47 |
| Msa1015950 | Msa1136420 | 0.804636 | 1.964243e-49 | -8.615850e-47 |
| Msa1015950 | Msa1285930 | 0.812323 | 4.492800e-51 | -8.615850e-47 |
| Msa1015950 | Msa1297210 | 0.803239 | 3.833023e-49 | -8.615850e-47 |
| Msa1015950 | Msa1340590 | 0.801216 | 9.995548e-49 | -8.615850e-47 |
| Msa1015950 | Msa1422180 | 0.800566 | 1.357263e-48 | -8.615850e-47 |
| Msa0846210 | Msa1015950 | 0.811725 | 6.063204e-51 | -8.615850e-47 |
| Msa0846350 | Msa1015950 | 0.803059 | 4.176309e-49 | -8.615850e-47 |
| Msa0852410 | Msa1015950 | 0.802574 | 5.258928e-49 | -8.615850e-47 |
| Msa0885290 | Msa1015950 | 0.838514 | 2.748885e-57 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTAATTCTATAAGTTAAA+AGG | 0.207748 | 7_1:+30860971 | None:intergenic |
| GGTCCTGATGCTGGAGATTC+AGG | 0.280201 | 7_1:-30861325 | Msa1015950:CDS |
| TGCAACACTGCCACCGAGTT+TGG | 0.303498 | 7_1:-30861437 | Msa1015950:CDS |
| ATCCTAGAGCTACGTGTAAT+TGG | 0.309322 | 7_1:-30861234 | Msa1015950:CDS |
| ACTAATGAGAGTAGAATAAC+TGG | 0.319837 | 7_1:-30861502 | Msa1015950:CDS |
| GAAGGATCAAGAGACTCTTT+TGG | 0.324518 | 7_1:+30861127 | None:intergenic |
| TACATGCTTCAGCATCAAAA+TGG | 0.341245 | 7_1:+30861032 | None:intergenic |
| GAGCAAATGGATTCTTAGAT+TGG | 0.344996 | 7_1:+30861168 | None:intergenic |
| CACGTAGCTCTAGGATTAGT+TGG | 0.358166 | 7_1:+30861241 | None:intergenic |
| AGGTTCAGCAGTAGAGTTCA+TGG | 0.387049 | 7_1:+30861468 | None:intergenic |
| GATAAGAAGAAAAGGAAAGT+TGG | 0.392292 | 7_1:-30861376 | Msa1015950:CDS |
| AAGCTTGTAGAACTAGATCT+TGG | 0.394131 | 7_1:-30861294 | Msa1015950:CDS |
| TTCAACAAATGCAGCTATCA+AGG | 0.414684 | 7_1:-30861347 | Msa1015950:CDS |
| AGAGTTAGTGTTTGTTGAGA+AGG | 0.444462 | 7_1:+30861109 | None:intergenic |
| GCTATCAAGGGTCCTGATGC+TGG | 0.445577 | 7_1:-30861334 | Msa1015950:CDS |
| AAAGAAGATGGGAATGGAAC+TGG | 0.465979 | 7_1:-30861073 | Msa1015950:CDS |
| GTACCTGAATCTCCAGCATC+AGG | 0.482272 | 7_1:+30861322 | None:intergenic |
| GTTCCAATGATAAGAAGAAA+AGG | 0.486743 | 7_1:-30861384 | Msa1015950:CDS |
| AGCTTGTAGAACTAGATCTT+GGG | 0.502321 | 7_1:-30861293 | Msa1015950:CDS |
| GTAGAACTAGATCTTGGGTC+TGG | 0.516938 | 7_1:-30861288 | Msa1015950:CDS |
| ATGAACTCTACTGCTGAACC+TGG | 0.520819 | 7_1:-30861466 | Msa1015950:CDS |
| GGCAGTGTTGCAAGTAGTCC+AGG | 0.524119 | 7_1:+30861448 | None:intergenic |
| AACACTGCCACCGAGTTTGG+AGG | 0.543171 | 7_1:-30861434 | Msa1015950:CDS |
| ACACTGCCACCGAGTTTGGA+GGG | 0.556428 | 7_1:-30861433 | Msa1015950:CDS |
| AATTATGTGAACGTTGGTGA+AGG | 0.566494 | 7_1:-30861406 | Msa1015950:CDS |
| TTTATGTGAATCAACAGCAT+AGG | 0.576652 | 7_1:+30861201 | None:intergenic |
| CACCAATTACACGTAGCTCT+AGG | 0.580322 | 7_1:+30861232 | None:intergenic |
| TCAACAAATGCAGCTATCAA+GGG | 0.590913 | 7_1:-30861346 | Msa1015950:CDS |
| CACTGCCACCGAGTTTGGAG+GGG | 0.599407 | 7_1:-30861432 | Msa1015950:CDS |
| AGCTACGTGTAATTGGTGTG+CGG | 0.672582 | 7_1:-30861227 | Msa1015950:CDS |
| CTAATGAGAGTAGAATAACT+GGG | 0.678598 | 7_1:-30861501 | Msa1015950:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATTTGTTAATTCGTATG+TGG | - | chr7_1:30861009-30861028 | Msa1015950:CDS | 20.0% |
| !! | AACAATTGAAAAAAGAAGAT+GGG | - | chr7_1:30861476-30861495 | Msa1015950:CDS | 20.0% |
| !! | TTTCCTTTTCTTCTTATCAT+TGG | + | chr7_1:30861182-30861201 | None:intergenic | 25.0% |
| ! | CAACAATTGAAAAAAGAAGA+TGG | - | chr7_1:30861475-30861494 | Msa1015950:CDS | 25.0% |
| ACTAATGAGAGTAGAATAAC+TGG | - | chr7_1:30861058-30861077 | Msa1015950:CDS | 30.0% | |
| CTAATGAGAGTAGAATAACT+GGG | - | chr7_1:30861059-30861078 | Msa1015950:CDS | 30.0% | |
| GTTCCAATGATAAGAAGAAA+AGG | - | chr7_1:30861176-30861195 | Msa1015950:CDS | 30.0% | |
| GATAAGAAGAAAAGGAAAGT+TGG | - | chr7_1:30861184-30861203 | Msa1015950:CDS | 30.0% | |
| TTTATGTGAATCAACAGCAT+AGG | + | chr7_1:30861362-30861381 | None:intergenic | 30.0% | |
| !! | GCTGTTGATTCACATAAAAA+TGG | - | chr7_1:30861364-30861383 | Msa1015950:CDS | 30.0% |
| !! | TTTTACATTGACTGAGCAAA+TGG | + | chr7_1:30861408-30861427 | None:intergenic | 30.0% |
| TTGAAAAAAGAAGATGGGAA+TGG | - | chr7_1:30861481-30861500 | Msa1015950:CDS | 30.0% | |
| ! | TAATTTTTCCCCTCCAAACT+CGG | + | chr7_1:30861139-30861158 | None:intergenic | 35.0% |
| GGGAAAAATTATGTGAACGT+TGG | - | chr7_1:30861148-30861167 | Msa1015950:CDS | 35.0% | |
| ! | AATTATGTGAACGTTGGTGA+AGG | - | chr7_1:30861154-30861173 | Msa1015950:CDS | 35.0% |
| TTCAACAAATGCAGCTATCA+AGG | - | chr7_1:30861213-30861232 | Msa1015950:CDS | 35.0% | |
| TCAACAAATGCAGCTATCAA+GGG | - | chr7_1:30861214-30861233 | Msa1015950:CDS | 35.0% | |
| AAGCTTGTAGAACTAGATCT+TGG | - | chr7_1:30861266-30861285 | Msa1015950:CDS | 35.0% | |
| AGCTTGTAGAACTAGATCTT+GGG | - | chr7_1:30861267-30861286 | Msa1015950:CDS | 35.0% | |
| ! | GAGCAAATGGATTCTTAGAT+TGG | + | chr7_1:30861395-30861414 | None:intergenic | 35.0% |
| ! | AGAGTTAGTGTTTGTTGAGA+AGG | + | chr7_1:30861454-30861473 | None:intergenic | 35.0% |
| TACATGCTTCAGCATCAAAA+TGG | + | chr7_1:30861531-30861550 | None:intergenic | 35.0% | |
| !! | ATTTTGATGCTGAAGCATGT+AGG | - | chr7_1:30861530-30861549 | Msa1015950:intron | 35.0% |
| ! | TTCGTATGTGGATGTTTTGC+AGG | - | chr7_1:30861021-30861040 | Msa1015950:CDS | 40.0% |
| ATCCTAGAGCTACGTGTAAT+TGG | - | chr7_1:30861326-30861345 | Msa1015950:CDS | 40.0% | |
| ! | GAAGGATCAAGAGACTCTTT+TGG | + | chr7_1:30861436-30861455 | None:intergenic | 40.0% |
| AAAGAAGATGGGAATGGAAC+TGG | - | chr7_1:30861487-30861506 | Msa1015950:CDS | 40.0% | |
| AGGTTCAGCAGTAGAGTTCA+TGG | + | chr7_1:30861095-30861114 | None:intergenic | 45.0% | |
| ATGAACTCTACTGCTGAACC+TGG | - | chr7_1:30861094-30861113 | Msa1015950:CDS | 45.0% | |
| GTAGAACTAGATCTTGGGTC+TGG | - | chr7_1:30861272-30861291 | Msa1015950:CDS | 45.0% | |
| !! | CACGTAGCTCTAGGATTAGT+TGG | + | chr7_1:30861322-30861341 | None:intergenic | 45.0% |
| CACCAATTACACGTAGCTCT+AGG | + | chr7_1:30861331-30861350 | None:intergenic | 45.0% | |
| AGCTACGTGTAATTGGTGTG+CGG | - | chr7_1:30861333-30861352 | Msa1015950:CDS | 45.0% | |
| ! | TTTTTCCCCTCCAAACTCGG+TGG | + | chr7_1:30861136-30861155 | None:intergenic | 50.0% |
| GTACCTGAATCTCCAGCATC+AGG | + | chr7_1:30861241-30861260 | None:intergenic | 50.0% | |
| GGCAGTGTTGCAAGTAGTCC+AGG | + | chr7_1:30861115-30861134 | None:intergenic | 55.0% | |
| TGCAACACTGCCACCGAGTT+TGG | - | chr7_1:30861123-30861142 | Msa1015950:CDS | 55.0% | |
| AACACTGCCACCGAGTTTGG+AGG | - | chr7_1:30861126-30861145 | Msa1015950:CDS | 55.0% | |
| ACACTGCCACCGAGTTTGGA+GGG | - | chr7_1:30861127-30861146 | Msa1015950:CDS | 55.0% | |
| GCTATCAAGGGTCCTGATGC+TGG | - | chr7_1:30861226-30861245 | Msa1015950:CDS | 55.0% | |
| GGTCCTGATGCTGGAGATTC+AGG | - | chr7_1:30861235-30861254 | Msa1015950:CDS | 55.0% | |
| CACTGCCACCGAGTTTGGAG+GGG | - | chr7_1:30861128-30861147 | Msa1015950:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 30860979 | 30861603 | 30860979 | ID=Msa1015950;Name=Msa1015950 |
| chr7_1 | mRNA | 30860979 | 30861603 | 30860979 | ID=Msa1015950-mRNA-1;Parent=Msa1015950;Name=Msa1015950-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0|0|0.5|1|1|2|0|188 |
| chr7_1 | exon | 30861598 | 30861603 | 30861598 | ID=Msa1015950-mRNA-1:exon:6174;Parent=Msa1015950-mRNA-1 |
| chr7_1 | exon | 30860979 | 30861539 | 30860979 | ID=Msa1015950-mRNA-1:exon:6173;Parent=Msa1015950-mRNA-1 |
| chr7_1 | CDS | 30861598 | 30861603 | 30861598 | ID=Msa1015950-mRNA-1:cds;Parent=Msa1015950-mRNA-1 |
| chr7_1 | CDS | 30860979 | 30861539 | 30860979 | ID=Msa1015950-mRNA-1:cds;Parent=Msa1015950-mRNA-1 |
| Gene Sequence |
| Protein sequence |
