Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1136420 | AES77518.1 | 90.097 | 616 | 49 | 1 | 1 | 616 | 1 | 604 | 0.0 | 1107 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1136420 | sp|P09189|HSP7C_PETHY | 65.802 | 617 | 206 | 4 | 1 | 614 | 1 | 615 | 0.0 | 825 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0131770 | Msa1136420 | 0.821767 | 3.388252e-53 | -8.615850e-47 |
| Msa0257320 | Msa1136420 | 0.803421 | 3.513780e-49 | -8.615850e-47 |
| Msa0290870 | Msa1136420 | 0.800504 | 1.397506e-48 | -8.615850e-47 |
| Msa0369460 | Msa1136420 | 0.809045 | 2.297283e-50 | -8.615850e-47 |
| Msa0626410 | Msa1136420 | 0.802445 | 5.592237e-49 | -8.615850e-47 |
| Msa0768370 | Msa1136420 | 0.806099 | 9.697220e-50 | -8.615850e-47 |
| Msa0899430 | Msa1136420 | 0.801427 | 9.052045e-49 | -8.615850e-47 |
| Msa0960640 | Msa1136420 | 0.803904 | 2.789495e-49 | -8.615850e-47 |
| Msa0963070 | Msa1136420 | 0.806407 | 8.351629e-50 | -8.615850e-47 |
| Msa0974080 | Msa1136420 | 0.839058 | 1.988412e-57 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1136420 | MtrunA17_Chr7g0217971 | 88.312 | 616 | 48 | 1 | 1 | 616 | 1 | 592 | 0.0 | 1081 |
| Msa1136420 | MtrunA17_Chr5g0427901 | 79.459 | 628 | 117 | 2 | 1 | 616 | 1 | 628 | 0.0 | 1021 |
| Msa1136420 | MtrunA17_Chr7g0223801 | 79.545 | 616 | 125 | 1 | 1 | 616 | 1 | 615 | 0.0 | 1009 |
| Msa1136420 | MtrunA17_Chr7g0221661 | 76.998 | 613 | 140 | 1 | 1 | 612 | 1 | 613 | 0.0 | 982 |
| Msa1136420 | MtrunA17_Chr2g0298851 | 77.508 | 618 | 135 | 4 | 1 | 615 | 1 | 617 | 0.0 | 981 |
| Msa1136420 | MtrunA17_Chr4g0040931 | 75.450 | 611 | 148 | 2 | 1 | 609 | 1 | 611 | 0.0 | 947 |
| Msa1136420 | MtrunA17_Chr2g0293581 | 73.667 | 619 | 159 | 4 | 1 | 615 | 1 | 619 | 0.0 | 944 |
| Msa1136420 | MtrunA17_Chr7g0225671 | 75.122 | 615 | 148 | 4 | 3 | 615 | 12 | 623 | 0.0 | 935 |
| Msa1136420 | MtrunA17_Chr7g0223721 | 73.258 | 617 | 163 | 2 | 1 | 615 | 1 | 617 | 0.0 | 932 |
| Msa1136420 | MtrunA17_Chr7g0233311 | 72.654 | 618 | 166 | 2 | 1 | 615 | 1 | 618 | 0.0 | 932 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1136420 | AT3G12580.1 | 65.478 | 617 | 208 | 4 | 1 | 614 | 1 | 615 | 0.0 | 823 |
| Msa1136420 | AT5G02500.1 | 64.668 | 617 | 213 | 4 | 1 | 614 | 1 | 615 | 0.0 | 816 |
| Msa1136420 | AT5G02490.1 | 64.344 | 617 | 215 | 4 | 1 | 614 | 1 | 615 | 0.0 | 809 |
| Msa1136420 | AT3G09440.1 | 64.506 | 617 | 214 | 4 | 1 | 614 | 1 | 615 | 0.0 | 802 |
| Msa1136420 | AT3G09440.4 | 64.506 | 617 | 214 | 4 | 1 | 614 | 1 | 615 | 0.0 | 802 |
| Msa1136420 | AT3G09440.2 | 64.506 | 617 | 214 | 4 | 1 | 614 | 1 | 615 | 0.0 | 802 |
| Msa1136420 | AT3G09440.3 | 64.506 | 617 | 214 | 4 | 1 | 614 | 1 | 615 | 0.0 | 802 |
| Msa1136420 | AT1G56410.1 | 64.668 | 617 | 211 | 5 | 1 | 614 | 1 | 613 | 0.0 | 800 |
| Msa1136420 | AT1G16030.1 | 62.561 | 617 | 225 | 5 | 1 | 614 | 1 | 614 | 0.0 | 789 |
| Msa1136420 | AT5G28540.1 | 54.501 | 611 | 267 | 8 | 7 | 612 | 35 | 639 | 0.0 | 639 |
Find 108 sgRNAs with CRISPR-Local
Find 272 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTACAACAACTATTGC+AGG | 0.418027 | 7_4:-4893129 | Msa1136420:CDS |
| AAAGATGCGTGAGACTGCAG+AGG | 0.681002 | 7_4:-4893792 | Msa1136420:CDS |
| AAAGTATGAAGGACGTGCAG+TGG | 0.587167 | 7_4:-4894343 | Msa1136420:CDS |
| AAATAAAGAATCTACCTCAA+TGG | 0.513375 | 7_4:+4893319 | None:intergenic |
| AACATCTATTTCACTCTGTT+GGG | 0.414811 | 7_4:+4892419 | None:intergenic |
| AAGTATGAAGGACGTGCAGT+GGG | 0.586872 | 7_4:-4894342 | Msa1136420:CDS |
| AATAGCACCAGCATCTATTG+TGG | 0.610874 | 7_4:+4893697 | None:intergenic |
| AATCACTCGTGCCAAGTTTG+AGG | 0.476109 | 7_4:-4893273 | Msa1136420:CDS |
| AATCTCATTCGTATTACCAG+TGG | 0.603979 | 7_4:+4892689 | None:intergenic |
| ACATCTTGCATCACCAAGTT+TGG | 0.348190 | 7_4:+4892999 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTTCACTGATGATCAAAGAT+TGG | - | chr7_4:4892499-4892518 | Msa1136420:CDS | 30.0% | |
| TTAGATGCAAAGAGGTTAAT+TGG | - | chr7_4:4892739-4892758 | Msa1136420:CDS | 30.0% | |
| TTGTACTTAACCGAAATCAA+GGG | + | chr7_4:4892844-4892863 | None:intergenic | 30.0% | |
| TTGATTTCGGTTAAGTACAA+GGG | - | chr7_4:4892844-4892863 | Msa1136420:CDS | 30.0% | |
| ATCATTGAAATAAGCAGGTA+CGG | + | chr7_4:4892977-4892996 | None:intergenic | 30.0% | |
| CTTATCGTTAATTGTAAGGA+GGG | + | chr7_4:4893172-4893191 | None:intergenic | 30.0% | |
| AGACCTTATCGTTAATTGTA+AGG | + | chr7_4:4893176-4893195 | None:intergenic | 30.0% | |
| TAGTAAAGCATACCTCTAAA+GGG | + | chr7_4:4893956-4893975 | None:intergenic | 30.0% | |
| ATAGTAAAGCATACCTCTAA+AGG | + | chr7_4:4893957-4893976 | None:intergenic | 30.0% | |
| AACATCTATTTCACTCTGTT+GGG | + | chr7_4:4894291-4894310 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 4892356 | 4894373 | 4892356 | ID=Msa1136420;Name=Msa1136420 |
| chr7_4 | mRNA | 4892356 | 4894373 | 4892356 | ID=Msa1136420-mRNA-1;Parent=Msa1136420;Name=Msa1136420-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|2|0|616 |
| chr7_4 | exon | 4894154 | 4894373 | 4894154 | ID=Msa1136420-mRNA-1:exon:1305;Parent=Msa1136420-mRNA-1 |
| chr7_4 | exon | 4892356 | 4893986 | 4892356 | ID=Msa1136420-mRNA-1:exon:1304;Parent=Msa1136420-mRNA-1 |
| chr7_4 | CDS | 4894154 | 4894373 | 4894154 | ID=Msa1136420-mRNA-1:cds;Parent=Msa1136420-mRNA-1 |
| chr7_4 | CDS | 4892356 | 4893986 | 4892356 | ID=Msa1136420-mRNA-1:cds;Parent=Msa1136420-mRNA-1 |
| Gene Sequence |
>Msa1136420 ATGGCAAAAAAGTATGAAGGACGTGCAGTGGGAATCGATCTTGGAACGACGTATTCGTGCGTTGCTGTGTGGCTAGATGAACACATCAGAGTTGAGATTATTCACAATGACCAAGGCAACAAAACCACACCTTCTTGTGTTGCTTTCACTGATGATCAAAGATTGGTTGGTGATGCAGCTAAGAATCAAGCTGCCACAAACCCTGAAAACACTGTTTTTGATGCAAAGAGGTTAATTGGTAGGAAGTTTGATGATTCTGTTGTTCAAAACGATATGATGCTCTGGCCATTCAAGGTCATTTCTGGAGTGAATGACAAACCCTTGATTTCGGTTAAGTACAAGGGACAGGAGATGCACCTATGTGCTGAAGAAATCTCATCTATGGTTCTAACAAAGATGCGTGAGACTGCAGAGGCATTTTTGGAATTGCCTGTCAAGAATGCAGTTGTTACCGTACCTGCTTATTTCAATGATGCTCAGCGGAAAGCCACAATAGATGCTGGTGCTATTGCTGGCCTTAATATTATGCGGATAATCAATGAGCCTACTGCTGCAGCTATTGCGTATGGACTGGACAAGAGAACTAATTGTGTGGAGGAGCGTAATATTTTCATCTTTGACTTGGGTGGTGGGACTTTTGATGTGTCCCTCCTTACAATTAACGATAAGGTCTTTCAAGTTAAGGCTACTGCCGGAAACACTCATCTTGGGGGAGAGGACATTGATAATAGGATGGTGAACTACTTTGCAAAGGAGTTTAAGAAGAAGAACAAGGTAGACATTAGTGAGAACGCAAGAGCCTTAAGGAGGTTGAGAACGGCGTGTGAAAGAGCAAAACGAATACTATCTTTCGCTGTCATCACCACCATTGAGGTAGATTCTTTATTTCAAGGCTTTGACTTCTTTTCATCAATCACTCGTGCCAAGTTTGAGGAAATAAATATGGATCTTTTCAACGAGTGTTTGGAAACTGTTGAGAGTTGTCTTGCTGATGCCAAAATGGACAAAAGTGCTATACATGATGTCGTCCTTGTCGGTGGCTCTTCTAGAATTCCAAAAGTACAACAACTATTGCAGGAATTTTTCGCAGGGAAGGATCTGTGTAAGAGCATCAACCCAGATGAGGCTGTTGCTTATGGAGCTGCTGTGCAAGCTGCTTTGTTGAGTGAAGGCTTTAAAAATGTACCAAACTTGGTGATGCAAGATGTTGCTCCTTTGTCTCTTGGTATCGAAGTTAATGATGATATAATGGGTGTTGTTATTGCTAGAAACACTAGCATTCCTATCAAGAAAACAAAAGGTTATTCAACGTCTAGTGATAACCAAACTTCGGTCCTCATCAATGTTTATGAGGGTGAGAGAGCAAGAGCTGGTGACAACAATCTGCTTGGTTCTTTTAGTCTTTCTGTCCCACGTGCTCCTCGTGGTCACCCTTTAGAGGTATGCTTTACTATCAATGAAAATGGCATCTTAATTGTTTCTGCAAATGAAAAATCCACTGGTAATACGAATGAGATTACAGTAACAAATGACAAAGAAAGATTGTCATCCCAAGAAATTACAAAAATGATCAGTGAAGCTGAAAATTACCGTGTTGAGGATGAAAAATTTCTAAGGAAGGCCAATGTAATGAATGCATTAGATTATTGTGTTTACAAGGTGAAAAATGCTTTGAAGAAGAAAGATGTGAATCTAAAGCTCTCTTCTCAAGAAAATGACGAGATCAATTCTGCAATCACAAAGGCCACAAGTTTACTTGATAGTACCCAACAGAGTGAAATAGATGTTCTTGAAAATCACCTGAAGGAGCTTGAGTGTATGCTCCAACTCATTATAAACAAATCTGTCTAG |
| Protein sequence |
>Msa1136420 MAKKYEGRAVGIDLGTTYSCVAVWLDEHIRVEIIHNDQGNKTTPSCVAFTDDQRLVGDAAKNQAATNPENTVFDAKRLIGRKFDDSVVQNDMMLWPFKVISGVNDKPLISVKYKGQEMHLCAEEISSMVLTKMRETAEAFLELPVKNAVVTVPAYFNDAQRKATIDAGAIAGLNIMRIINEPTAAAIAYGLDKRTNCVEERNIFIFDLGGGTFDVSLLTINDKVFQVKATAGNTHLGGEDIDNRMVNYFAKEFKKKNKVDISENARALRRLRTACERAKRILSFAVITTIEVDSLFQGFDFFSSITRAKFEEINMDLFNECLETVESCLADAKMDKSAIHDVVLVGGSSRIPKVQQLLQEFFAGKDLCKSINPDEAVAYGAAVQAALLSEGFKNVPNLVMQDVAPLSLGIEVNDDIMGVVIARNTSIPIKKTKGYSTSSDNQTSVLINVYEGERARAGDNNLLGSFSLSVPRAPRGHPLEVCFTINENGILIVSANEKSTGNTNEITVTNDKERLSSQEITKMISEAENYRVEDEKFLRKANVMNALDYCVYKVKNALKKKDVNLKLSSQENDEINSAITKATSLLDSTQQSEIDVLENHLKELECMLQLIINKSV* |
