AlfalfaGEDB | Msa0294200

M. sativa cultivar zhongmu-4 / Msa0294200

KEGG: -
GO: GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005829 GO:0006810 GO:0006833 GO:0008150 GO:0009505 GO:0012505 GO:0016020 GO:0030312 GO:0031090 GO:0042044 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048046 GO:0051179 GO:0051234 GO:0071944 GO:0098588 GO:0098805
PFAM: PF01453.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0294200	RDX74635.1	65.947	834	166	12	1	721	55	883	0.0	1085
Msa0294200	RDX74635.1	67.892	408	108	6	333	721	40	443	0.0	562

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0294200	sp\|Q9ZVA5\|EP1L4_ARATH	48.403	407	183	7	333	721	37	434	3.64e-125	383
Msa0294200	sp\|Q9ZVA5\|EP1L4_ARATH	45.333	375	157	8	5	333	56	428	1.60e-97	311

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0294200	A0A371F8L2	65.947	834	166	12	1	721	55	883	0.0	1085
Msa0294200	A0A371F8L2	67.892	408	108	6	333	721	40	443	0.0	562

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa0294200	-0.876158	1.743311e-68	-8.615850e-47
Msa0000800	Msa0294200	0.843005	1.827537e-58	-8.615850e-47
Msa0002110	Msa0294200	-0.849009	4.257123e-60	-8.615850e-47
Msa0002690	Msa0294200	-0.808947	2.410713e-50	-8.615850e-47
Msa0002700	Msa0294200	-0.819659	1.034146e-52	-8.615850e-47
Msa0004180	Msa0294200	-0.816092	6.609312e-52	-8.615850e-47
Msa0005500	Msa0294200	-0.843377	1.454447e-58	-8.615850e-47
Msa0006080	Msa0294200	0.871248	7.896996e-67	-8.615850e-47
Msa0007570	Msa0294200	-0.800181	1.625476e-48	-8.615850e-47
Msa0007810	Msa0294200	-0.866055	3.770948e-65	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0294200	MtrunA17_Chr2g0315841	82.367	414	66	1	316	722	23	436	0.0	700
Msa0294200	MtrunA17_Chr2g0315841	84.085	377	18	4	1	335	55	431	0.0	630
Msa0294200	MtrunA17_Chr2g0315851	81.250	416	69	2	316	722	23	438	0.0	673
Msa0294200	MtrunA17_Chr2g0315851	81.266	379	27	5	1	335	55	433	0.0	592
Msa0294200	MtrunA17_Chr2g0315861	88.095	168	19	1	506	673	5	171	6.51e-101	307
Msa0294200	MtrunA17_Chr2g0315861	70.175	171	23	3	148	291	2	171	3.85e-70	226
Msa0294200	MtrunA17_Chr2g0315871	62.934	259	71	4	331	580	5	247	6.89e-100	308
Msa0294200	MtrunA17_Chr2g0315871	68.696	230	39	5	1	213	22	235	1.95e-92	289
Msa0294200	MtrunA17_Chr1g0149381	24.854	342	234	9	384	719	90	414	1.04e-21	100
Msa0294200	MtrunA17_Chr1g0149381	25.205	365	207	13	9	330	65	406	4.44e-16	82.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0294200	AT1G78860.1	48.403	407	183	7	333	721	37	434	3.70e-126	383
Msa0294200	AT1G78860.1	45.333	375	157	8	5	333	56	428	1.62e-98	311
Msa0294200	AT1G78850.1	48.276	406	185	7	333	721	37	434	6.94e-125	379
Msa0294200	AT1G78850.1	45.479	376	155	10	5	333	56	428	1.46e-98	311
Msa0294200	AT1G16905.1	44.089	406	191	7	333	721	36	422	3.46e-110	341
Msa0294200	AT1G16905.1	38.933	375	176	9	2	333	52	416	1.39e-73	245
Msa0294200	AT1G78830.1	42.857	420	205	10	333	718	36	454	1.26e-101	320
Msa0294200	AT1G78830.1	39.599	399	172	11	3	335	58	453	4.39e-79	260
Msa0294200	AT1G78820.1	42.029	414	205	10	339	718	42	454	6.88e-98	310
Msa0294200	AT1G78820.1	38.000	400	177	12	3	335	58	453	1.40e-74	248

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Find 143 sgRNAs with CRISPR-Local

Find 462 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGCAACAACACCTATAT+TGG	0.309987	2_3:-45941982	None:intergenic
AAAGGCAACAACACCTTTAT+TGG	0.185484	2_3:-45935160	None:intergenic
AAATGTAGCCCTAAGCCTTT+AGG	0.350816	2_3:+45935804	Msa0294200:CDS
AACACCTTGAGTCATCCTTA+TGG	0.455333	2_3:-45942775	None:intergenic
AACCAATATTTAATTGGCCT+AGG	0.509751	2_3:-45935408	None:intergenic
AAGTGTAGTCCTAAGCCTTT+AGG	0.361000	2_3:+45942605	Msa0294200:CDS
AATATGGAGGTAATTACAGA+AGG	0.595926	2_3:+45941730	Msa0294200:CDS
AATCCATACTCAAAAGATTC+TGG	0.292898	2_3:-45935492	None:intergenic
AATCCATACTCAAATGATTC+TGG	0.297053	2_3:-45942293	None:intergenic
AATGATACTATTGCCTGAAG+TGG	0.563330	2_3:-45935538	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGTAAAAACCAACTTGGAA+TGG	-	chr2_3:45934952-45934971	None:intergenic	30.0%
TAGTGTTGTAAAAACCAACT+TGG	-	chr2_3:45934957-45934976	None:intergenic	30.0%
CAAACAAACACTTCCAATAA+AGG	+	chr2_3:45935147-45935166	Msa0294200:CDS	30.0%
AATCCATACTCAAAAGATTC+TGG	-	chr2_3:45935495-45935514	None:intergenic	30.0%
TTTGATGAAGATGAAGATGA+GGG	+	chr2_3:45935681-45935700	Msa0294200:CDS	30.0%
ATAAGCCTAGATTTCTTCTT+GGG	+	chr2_3:45936114-45936133	Msa0294200:intron	30.0%
ACCGACGCAAATAATTTAAA+TGG	-	chr2_3:45936298-45936317	None:intergenic	30.0%
CAAAATTTATTGTGGCTTGA+TGG	+	chr2_3:45936565-45936584	Msa0294200:intron	30.0%
TCAACATGGTCTAAATGATT+CGG	+	chr2_3:45936602-45936621	Msa0294200:intron	30.0%
CTAAGGTTAACCTTTATGAA+AGG	-	chr2_3:45936850-45936869	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	45934884	45942874	45934884	ID=Msa0294200;Name=Msa0294200
chr2_3	mRNA	45934884	45942874	45934884	ID=Msa0294200-mRNA-1;Parent=Msa0294200;Name=Msa0294200-mRNA-1;_AED=0.08;_eAED=0.10;_QI=44\|0\|0\|1\|0\|0\|6\|0\|722
chr2_3	exon	45934884	45935081	45934884	ID=Msa0294200-mRNA-1:exon:14371;Parent=Msa0294200-mRNA-1
chr2_3	exon	45935097	45935345	45935097	ID=Msa0294200-mRNA-1:exon:14372;Parent=Msa0294200-mRNA-1
chr2_3	exon	45935376	45935612	45935376	ID=Msa0294200-mRNA-1:exon:14373;Parent=Msa0294200-mRNA-1
chr2_3	exon	45935667	45935843	45935667	ID=Msa0294200-mRNA-1:exon:14374;Parent=Msa0294200-mRNA-1
chr2_3	exon	45935871	45936050	45935871	ID=Msa0294200-mRNA-1:exon:14375;Parent=Msa0294200-mRNA-1
chr2_3	exon	45941706	45942874	45941706	ID=Msa0294200-mRNA-1:exon:14376;Parent=Msa0294200-mRNA-1
chr2_3	five_prime_UTR	45934884	45934927	45934884	ID=Msa0294200-mRNA-1:five_prime_utr;Parent=Msa0294200-mRNA-1
chr2_3	CDS	45934928	45935081	45934928	ID=Msa0294200-mRNA-1:cds;Parent=Msa0294200-mRNA-1
chr2_3	CDS	45935097	45935345	45935097	ID=Msa0294200-mRNA-1:cds;Parent=Msa0294200-mRNA-1
chr2_3	CDS	45935376	45935612	45935376	ID=Msa0294200-mRNA-1:cds;Parent=Msa0294200-mRNA-1
chr2_3	CDS	45935667	45935843	45935667	ID=Msa0294200-mRNA-1:cds;Parent=Msa0294200-mRNA-1
chr2_3	CDS	45935871	45936050	45935871	ID=Msa0294200-mRNA-1:cds;Parent=Msa0294200-mRNA-1
chr2_3	CDS	45941706	45942874	45941706	ID=Msa0294200-mRNA-1:cds;Parent=Msa0294200-mRNA-1

Gene Sequence

>Msa0294200

ATGATAAGCATATTCAATGCTCCATTCCAAGTTGGTTTTTACAACACTACTCCAAATGCCTTCACTTTAGCTCTTAGAATCGGACTTCAACGATCGGAGCAGCTTTTCCGGTGGGTATGGGAAGCAAATAGAGGCAACCCAGTTGGTGAAAATGGTGCTGATGGAAATCTTGTGTTGGCTAATGCTGATGGAAGAATTGTTTGGCAAACAAACACTTCCAATAAAGGTGTTGTTGCCTTTAGGTTACTCTCAAATGGTAACATGGTTTTAATTGATTCTAAAGGTAAATTTGTTTGGCAAAGTTTTGATCATCCTACAGATACCCTTTTGGTGGATCAATATTTAAAACCTAATGGTCCTTCAAAACTTGTTAGTAGGCTTTCAGAAAAAGAAAATGTTGATGGTTTGGCTTTATATTATAAGAGTAAAAATTCTCCTAGGCCAATTAAATATTGGTTTTCATCTTCTTGGTTTTCATTTGAAAAAGGCTCATTAGAAAATGTCACACTAAAATCTGATCCAGAATCTTTTGAGTATGGATTTGATTCTTTTGTTGTCAATTCTACCACTTCAGGCAATAGTATCATTGGTAGGCCAGTGAATAATAGTACCTTAACATATCTTAGGCTTGGAATTGATGTGACATACACTCTCTTTGATGAAGATGAAGATGAGGGTGAGTGTCAATTGCCAGAGAGATGTGGAAAATTTGGACTTTGTGAGGATAATCAATGTGTTGCTTGTCCATTGGAGAATGGAATATTTGGTTGGAGTAATAAATGTAGCCCTAAGCCTTTAGGAGGAATATGCAAAGAAAGAGTTGAGCATTACATGAGCAAATACACTACTGGTGATAGTGTAAGTGAAGCTAATTGCGGAAATAAATGTAGCAAGGATTGTAAGTGTGTTGGGTATTTCTATCACAAGGATAACTCAAGGTGTTGGATAGCTTATGATCTTCAAACTCTTACTAGAGTTCAAAATACCACACACGTGGGTGCACTTGATGAATCCATTGTAGAATATGGAGGTAATTACAGAAGGATGGGCATAATCACTGTTTCATTCCAGGTTTGTTTTTACAACACTACCCAAAATGCCTTCACTCTAGCACTTCGAATGGGATTTGAAACGTCGGAAGAGCTTTTTCGGTGGGTTTGGGAAGCAAATAGAGGCAACCCAGTTGATGAAAATGCAACTTTTTCATTAGGTGTTGATGGAAATCTTGTGTTGGCTAATGCTGATGGAAGAATTGTTTGGCAAACAAACACTTCCAATATAGGTGTTGTTGCCTTTAGGTTACTCTCAAATGGTAACATGGTTTTAATTGATGCTAAAGGTAGTTTTGTTTGGCAAAGTTTTGATCATCCAACAGATACCCTTTTGGTTGATCAATATTTAAAACCTAATGGTCCTTCAAAACTTGTTAGTAGGCTTTCAGAAAAAGAAAATGTTGATGGTCCTTATAGTTTGGTTTTGGAGCCTAAAGGTTTGGCTTTGTATTATAAGAGTATATATTGGTTTTCATCTGCTTGGTTTTCATTTGAAAAAGGCTCACTAAAAAATGTCACACTCAAATCTGATCCAGAATCATTTGAGTATGGATTTGATTTTTTTGTTGCCAATTCTACCACTTTTGGTAAAAGTATCATTGGTAGGCCAGTGAAGAATAGTACTTTAACATATCTTAGGCTTGGAATTGATGGTAACATCAGATTTTACACATATTTTCTAGATGTTCAAGATGGTGTTTGGAAAGTGACATACACTCTCTTTGATAGAGATTTTGATGAGAGTGAGTGTCAATTGCCAGAGAGATGTGGGAAATTTGGGCTTTGTGAGGATAATCAATGTGTTGGTTGTCCTCTGGAGAATGGAATATTTGGTTGGAGTAATAAGTGTAGTCCTAAGCCTTTAGGAGGAGTATGCAATGCAAGTAAATTTCATTACTACAAAATTGAAGGAGTTGAGCATTATATGATCAAATACACTATTGGTGATGGAGTAAGCGAAGTTAATTGTGGAAACAAATGTACCAAGGATTGCAAGTGTGTTGGGTATTTCTACCATAAGGATGACTCAAGGTGTTGGATAGCTTATGATTTGCAAACTCTTACTAGAGTGGCAAATACTACGCATGTGGGTTATATCAAGGTGCCTAATAAG

Protein sequence

>Msa0294200

MISIFNAPFQVGFYNTTPNAFTLALRIGLQRSEQLFRWVWEANRGNPVGENGADGNLVLANADGRIVWQTNTSNKGVVAFRLLSNGNMVLIDSKGKFVWQSFDHPTDTLLVDQYLKPNGPSKLVSRLSEKENVDGLALYYKSKNSPRPIKYWFSSSWFSFEKGSLENVTLKSDPESFEYGFDSFVVNSTTSGNSIIGRPVNNSTLTYLRLGIDVTYTLFDEDEDEGECQLPERCGKFGLCEDNQCVACPLENGIFGWSNKCSPKPLGGICKERVEHYMSKYTTGDSVSEANCGNKCSKDCKCVGYFYHKDNSRCWIAYDLQTLTRVQNTTHVGALDESIVEYGGNYRRMGIITVSFQVCFYNTTQNAFTLALRMGFETSEELFRWVWEANRGNPVDENATFSLGVDGNLVLANADGRIVWQTNTSNIGVVAFRLLSNGNMVLIDAKGSFVWQSFDHPTDTLLVDQYLKPNGPSKLVSRLSEKENVDGPYSLVLEPKGLALYYKSIYWFSSAWFSFEKGSLKNVTLKSDPESFEYGFDFFVANSTTFGKSIIGRPVKNSTLTYLRLGIDGNIRFYTYFLDVQDGVWKVTYTLFDRDFDESECQLPERCGKFGLCEDNQCVGCPLENGIFGWSNKCSPKPLGGVCNASKFHYYKIEGVEHYMIKYTIGDGVSEVNCGNKCTKDCKCVGYFYHKDDSRCWIAYDLQTLTRVANTTHVGYIKVPNK