Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1048540 | XP_024626063.2 | 76.190 | 126 | 26 | 3 | 1 | 125 | 1 | 123 | 2.40e-37 | 142 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1048540 | G7KZX4 | 77.778 | 126 | 25 | 3 | 1 | 125 | 402 | 525 | 3.46e-37 | 144 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0023610 | Msa1048540 | 0.813301 | 2.742752e-51 | -8.615850e-47 |
| Msa0068630 | Msa1048540 | 0.849034 | 4.190221e-60 | -8.615850e-47 |
| Msa0071340 | Msa1048540 | 0.809947 | 1.470480e-50 | -8.615850e-47 |
| Msa0118050 | Msa1048540 | 0.801369 | 9.301143e-49 | -8.615850e-47 |
| Msa0675510 | Msa1048540 | 0.811027 | 8.596776e-51 | -8.615850e-47 |
| Msa0153200 | Msa1048540 | 0.812524 | 4.059957e-51 | -8.615850e-47 |
| Msa0162580 | Msa1048540 | 0.837186 | 6.030604e-57 | -8.615850e-47 |
| Msa0458420 | Msa1048540 | 0.832256 | 1.048830e-55 | -8.615850e-47 |
| Msa1006330 | Msa1048540 | 0.804654 | 1.947588e-49 | -8.615850e-47 |
| Msa1048540 | Msa1232060 | 0.819282 | 1.260392e-52 | -8.615850e-47 |
| Msa1048540 | Msa1463140 | 0.812381 | 4.361730e-51 | -8.615850e-47 |
| Msa0280450 | Msa1048540 | 0.817646 | 2.960050e-52 | -8.615850e-47 |
| Msa0962360 | Msa1048540 | 0.817800 | 2.732747e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1048540 | MtrunA17_Chr7g0217081 | 77.778 | 126 | 25 | 3 | 1 | 125 | 402 | 525 | 6.66e-41 | 144 |
| Msa1048540 | MtrunA17_Chr7g0217101 | 76.190 | 126 | 26 | 3 | 1 | 125 | 158 | 280 | 6.76e-41 | 142 |
| Msa1048540 | MtrunA17_Chr8g0357191 | 54.622 | 119 | 43 | 2 | 16 | 125 | 539 | 655 | 1.60e-31 | 117 |
| Msa1048540 | MtrunA17_Chr7g0218991 | 52.542 | 118 | 47 | 2 | 16 | 124 | 304 | 421 | 3.07e-28 | 107 |
| Msa1048540 | MtrunA17_Chr7g0215671 | 50.000 | 118 | 50 | 1 | 16 | 124 | 297 | 414 | 4.09e-27 | 104 |
| Msa1048540 | MtrunA17_Chr7g0215651 | 46.721 | 122 | 58 | 3 | 9 | 123 | 276 | 397 | 8.89e-25 | 97.8 |
| Msa1048540 | MtrunA17_Chr8g0357151 | 53.782 | 119 | 44 | 2 | 16 | 125 | 623 | 739 | 2.07e-24 | 97.1 |
| Msa1048540 | MtrunA17_Chr7g0215061 | 44.444 | 126 | 48 | 1 | 20 | 123 | 360 | 485 | 2.93e-24 | 96.7 |
| Msa1048540 | MtrunA17_Chr7g0215641 | 55.405 | 74 | 32 | 1 | 16 | 89 | 306 | 378 | 3.55e-22 | 90.5 |
| Msa1048540 | MtrunA17_Chr7g0215011 | 50.000 | 74 | 36 | 1 | 16 | 89 | 322 | 394 | 1.20e-18 | 80.5 |
| Msa1048540 | MtrunA17_Chr8g0357171 | 60.656 | 61 | 24 | 0 | 21 | 81 | 485 | 545 | 1.65e-18 | 80.1 |
| Msa1048540 | MtrunA17_Chr7g0217061 | 47.368 | 76 | 38 | 1 | 16 | 89 | 319 | 394 | 1.29e-15 | 71.6 |
| Msa1048540 | MtrunA17_Chr7g0215631 | 60.938 | 64 | 25 | 0 | 20 | 83 | 294 | 357 | 2.52e-15 | 70.9 |
| Msa1048540 | MtrunA17_Chr7g0215681 | 48.718 | 117 | 49 | 4 | 20 | 125 | 330 | 446 | 1.89e-14 | 68.6 |
| Msa1048540 | MtrunA17_Chr7g0216261 | 60.317 | 63 | 25 | 0 | 17 | 79 | 217 | 279 | 6.70e-13 | 63.9 |
| Msa1048540 | MtrunA17_Chr4g0073471 | 45.946 | 74 | 34 | 2 | 20 | 87 | 121 | 194 | 7.31e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1048540 | AT5G16000.1 | 43.243 | 74 | 36 | 2 | 20 | 87 | 133 | 206 | 3.28e-11 | 59.7 |
Find 29 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTTCCTTCAATCATTTAA+AGG | 0.211915 | 7_2:+10315453 | Msa1048540:CDS |
| TTTCATCTAAACAACTTTCA+TGG | 0.348751 | 7_2:+10315269 | Msa1048540:CDS |
| TTAAATAATAATAATCTCAT+TGG | 0.349276 | 7_2:+10315391 | Msa1048540:CDS |
| ATCGTTATAACACAATAATA+TGG | 0.371760 | 7_2:+10315497 | Msa1048540:CDS |
| TGAAGGAAATGTCTACATAA+TGG | 0.379369 | 7_2:-10315441 | None:intergenic |
| ATTTCCTTCAATCATTTAAA+GGG | 0.392069 | 7_2:+10315454 | Msa1048540:CDS |
| ATGGATATTGCAAAGAGATT+GGG | 0.394817 | 7_2:-10315422 | None:intergenic |
| AATGGATATTGCAAAGAGAT+TGG | 0.401172 | 7_2:-10315423 | None:intergenic |
| CGATCAGGACAGTCTGGAAT+AGG | 0.419109 | 7_2:-10315478 | None:intergenic |
| ATTATTGTGTTATAACGATC+AGG | 0.444685 | 7_2:-10315493 | None:intergenic |
| GACCTCAGTTACAACCTCAT+TGG | 0.453057 | 7_2:+10315325 | Msa1048540:CDS |
| GGTGAAATCCCTTCTCAACT+TGG | 0.453729 | 7_2:+10315349 | Msa1048540:CDS |
| ATGAAAGTTGTTTAGATGAA+AGG | 0.471661 | 7_2:-10315267 | None:intergenic |
| TGAAAATATCCAAGTTGAGA+AGG | 0.473369 | 7_2:-10315358 | None:intergenic |
| AAAGTGAGAAGGCGAAGATC+AGG | 0.483217 | 7_2:-10315597 | None:intergenic |
| AGGACCCTTTAAATGATTGA+AGG | 0.490153 | 7_2:-10315458 | None:intergenic |
| GAAAATATCCAAGTTGAGAA+GGG | 0.506328 | 7_2:-10315357 | None:intergenic |
| TGGATATTGCAAAGAGATTG+GGG | 0.509445 | 7_2:-10315421 | None:intergenic |
| TTATAACGATCAGGACAGTC+TGG | 0.509894 | 7_2:-10315484 | None:intergenic |
| TTATATTATTACTTCCGTTG+TGG | 0.541085 | 7_2:-10315537 | None:intergenic |
| CTCAGTTACAACCTCATTGG+AGG | 0.546682 | 7_2:+10315328 | Msa1048540:CDS |
| AAGATCAGGATCAGAAGGAT+AGG | 0.551987 | 7_2:-10315583 | None:intergenic |
| GAAGCATATTAAAAGTGAGA+AGG | 0.561477 | 7_2:-10315608 | None:intergenic |
| AGGCGAAGATCAGGATCAGA+AGG | 0.573796 | 7_2:-10315588 | None:intergenic |
| AAGGGATTTCACCTCCAATG+AGG | 0.626407 | 7_2:-10315339 | None:intergenic |
| CTCCAATGAGGTTGTAACTG+AGG | 0.641568 | 7_2:-10315327 | None:intergenic |
| GAGGTTGTAACTGAGGTCAA+CGG | 0.660516 | 7_2:-10315320 | None:intergenic |
| GACGATGCATATATCCACAA+CGG | 0.723123 | 7_2:+10315523 | Msa1048540:CDS |
| GGATATTGCAAAGAGATTGG+GGG | 0.723214 | 7_2:-10315420 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAATAATAATAATCTCAT+TGG | + | chr7_2:10315391-10315410 | Msa1048540:CDS | 10.0% |
| !! | ATTTCCTTCAATCATTTAAA+GGG | + | chr7_2:10315454-10315473 | Msa1048540:CDS | 20.0% |
| !! | ATCGTTATAACACAATAATA+TGG | + | chr7_2:10315497-10315516 | Msa1048540:CDS | 20.0% |
| ! | ATGAAAGTTGTTTAGATGAA+AGG | - | chr7_2:10315270-10315289 | None:intergenic | 25.0% |
| ! | TTTCATCTAAACAACTTTCA+TGG | + | chr7_2:10315269-10315288 | Msa1048540:CDS | 25.0% |
| ! | CATTTCCTTCAATCATTTAA+AGG | + | chr7_2:10315453-10315472 | Msa1048540:CDS | 25.0% |
| ! | ATTATTGTGTTATAACGATC+AGG | - | chr7_2:10315496-10315515 | None:intergenic | 25.0% |
| ! | TTATATTATTACTTCCGTTG+TGG | - | chr7_2:10315540-10315559 | None:intergenic | 25.0% |
| ! | GAAAATATCCAAGTTGAGAA+GGG | - | chr7_2:10315360-10315379 | None:intergenic | 30.0% |
| ! | TGAAAATATCCAAGTTGAGA+AGG | - | chr7_2:10315361-10315380 | None:intergenic | 30.0% |
| ATGGATATTGCAAAGAGATT+GGG | - | chr7_2:10315425-10315444 | None:intergenic | 30.0% | |
| AATGGATATTGCAAAGAGAT+TGG | - | chr7_2:10315426-10315445 | None:intergenic | 30.0% | |
| TGAAGGAAATGTCTACATAA+TGG | - | chr7_2:10315444-10315463 | None:intergenic | 30.0% | |
| GAAGCATATTAAAAGTGAGA+AGG | - | chr7_2:10315611-10315630 | None:intergenic | 30.0% | |
| TGGATATTGCAAAGAGATTG+GGG | - | chr7_2:10315424-10315443 | None:intergenic | 35.0% | |
| AGGACCCTTTAAATGATTGA+AGG | - | chr7_2:10315461-10315480 | None:intergenic | 35.0% | |
| GGATATTGCAAAGAGATTGG+GGG | - | chr7_2:10315423-10315442 | None:intergenic | 40.0% | |
| TTATAACGATCAGGACAGTC+TGG | - | chr7_2:10315487-10315506 | None:intergenic | 40.0% | |
| GACGATGCATATATCCACAA+CGG | + | chr7_2:10315523-10315542 | Msa1048540:CDS | 40.0% | |
| AAGATCAGGATCAGAAGGAT+AGG | - | chr7_2:10315586-10315605 | None:intergenic | 40.0% | |
| GAGGTTGTAACTGAGGTCAA+CGG | - | chr7_2:10315323-10315342 | None:intergenic | 45.0% | |
| GACCTCAGTTACAACCTCAT+TGG | + | chr7_2:10315325-10315344 | Msa1048540:CDS | 45.0% | |
| CTCCAATGAGGTTGTAACTG+AGG | - | chr7_2:10315330-10315349 | None:intergenic | 45.0% | |
| CTCAGTTACAACCTCATTGG+AGG | + | chr7_2:10315328-10315347 | Msa1048540:CDS | 45.0% | |
| AAGGGATTTCACCTCCAATG+AGG | - | chr7_2:10315342-10315361 | None:intergenic | 45.0% | |
| GGTGAAATCCCTTCTCAACT+TGG | + | chr7_2:10315349-10315368 | Msa1048540:CDS | 45.0% | |
| AAAGTGAGAAGGCGAAGATC+AGG | - | chr7_2:10315600-10315619 | None:intergenic | 45.0% | |
| ! | CGATCAGGACAGTCTGGAAT+AGG | - | chr7_2:10315481-10315500 | None:intergenic | 50.0% |
| AGGCGAAGATCAGGATCAGA+AGG | - | chr7_2:10315591-10315610 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 10315262 | 10315639 | 10315262 | ID=Msa1048540;Name=Msa1048540 |
| chr7_2 | mRNA | 10315262 | 10315639 | 10315262 | ID=Msa1048540-mRNA-1;Parent=Msa1048540;Name=Msa1048540-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|125 |
| chr7_2 | exon | 10315262 | 10315639 | 10315262 | ID=Msa1048540-mRNA-1:exon:2752;Parent=Msa1048540-mRNA-1 |
| chr7_2 | CDS | 10315262 | 10315639 | 10315262 | ID=Msa1048540-mRNA-1:cds;Parent=Msa1048540-mRNA-1 |
| Gene Sequence |
| Protein sequence |
