AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1101880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1101880 MtrunA17_Chr7g0231021 55.411 231 82 6 1 227 13 226 1.76e-75 228
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 52 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATTATGTCCAGTAATTA+TGG 0.204048 7_3:-32995034 Msa1101880:three_prime_UTR
TTCTTAGATCTTCTGGTTGT+TGG 0.220722 7_3:-32995125 Msa1101880:three_prime_UTR
GCTATATTCCTCTTCAGTTT+TGG 0.242171 7_3:+32995885 None:intergenic
ATTCTCTTTCTTAGATCTTC+TGG 0.262659 7_3:-32995132 Msa1101880:three_prime_UTR
ATGTCCAGTAATTATGGTTT+TGG 0.291559 7_3:-32995028 Msa1101880:three_prime_UTR
CCTGCCTTCCAGTAAGATTA+GGG 0.305278 7_3:-32996035 Msa1101880:five_prime_UTR
GAGATAAACCCTAATCTTAC+TGG 0.306987 7_3:+32996027 None:intergenic
GCAGATCTCATTGGTGATAA+TGG 0.313512 7_3:-32995935 Msa1101880:intron
AGAAACAAGGGGCTGGCTTT+TGG 0.324875 7_3:-32996189 Msa1101880:five_prime_UTR
TCCTGCCTTCCAGTAAGATT+AGG 0.337836 7_3:-32996036 Msa1101880:five_prime_UTR
TCTTAGATCTTCTGGTTGTT+GGG 0.338515 7_3:-32995124 Msa1101880:three_prime_UTR
TCAAATTCAAAGTTAGGTAT+AGG 0.342324 7_3:-32995360 Msa1101880:CDS
AATTCCAAAACCATAATTAC+TGG 0.347209 7_3:+32995024 None:intergenic
TTTGAATCACAAATGGTTAA+GGG 0.392081 7_3:-32995517 Msa1101880:CDS
AATGAAGACTTCTCTTAAAC+CGG 0.394736 7_3:-32995323 Msa1101880:CDS
AAGAAAGAGAATGAAGCATT+TGG 0.402743 7_3:+32995143 None:intergenic
AGCTTTATCTCTCGTTGATC+CGG 0.408339 7_3:-32995911 Msa1101880:CDS
TAATCTTGTCTGAAGTTGAA+TGG 0.419438 7_3:+32995778 None:intergenic
TCTAGCAAATTACAATGGTT+TGG 0.420392 7_3:-32995446 Msa1101880:CDS
AATCTAATTATCAGAGCTAT+AGG 0.425590 7_3:-32995236 Msa1101880:exon
TACGGTTGGTGGTCTTATAC+TGG 0.432407 7_3:+32995851 None:intergenic
AGCTCTGATAATTAGATTTG+AGG 0.439710 7_3:+32995241 None:intergenic
AGCCCCTTGTTTCTGCTGTA+CGG 0.443886 7_3:+32996198 None:intergenic
TACTTTCGTATGCTTTGTGT+TGG 0.464331 7_3:-32994936 Msa1101880:three_prime_UTR
TTTAATTTAGCAGATCTCAT+TGG 0.466960 7_3:-32995944 Msa1101880:intron
AAGAACTTGATAAAGAAAAC+AGG 0.469680 7_3:+32996163 None:intergenic
CCCTAATCTTACTGGAAGGC+AGG 0.469877 7_3:+32996035 None:intergenic
TTGAATCACAAATGGTTAAG+GGG 0.495749 7_3:-32995516 Msa1101880:CDS
ATTCGTGCTTGTAAACTCAC+TGG 0.507964 7_3:-32994887 Msa1101880:three_prime_UTR
AGCAAATTACAATGGTTTGG+TGG 0.509594 7_3:-32995443 Msa1101880:CDS
TGCTTATAAAAGTTACCGAT+GGG 0.519413 7_3:+32995814 None:intergenic
ATCATGATCCTGATCCATCA+TGG 0.523877 7_3:+32995624 None:intergenic
AGCTATAGGTGTAAAATGAA+TGG 0.526560 7_3:-32995222 Msa1101880:three_prime_UTR
TTGCTTATAAAAGTTACCGA+TGG 0.527123 7_3:+32995813 None:intergenic
AAGTCTTCAAGACTCTATCT+TGG 0.527360 7_3:-32995545 Msa1101880:CDS
AGATAGAGTCTTGAAGACTT+TGG 0.529629 7_3:+32995548 None:intergenic
AGAATGAAGCATTTGGCTTG+TGG 0.544416 7_3:+32995150 None:intergenic
ACCACCGTACAGCAGAAACA+AGG 0.550982 7_3:-32996202 None:intergenic
AATCTTACTGGAAGGCAGGA+AGG 0.552923 7_3:+32996039 None:intergenic
CTTTCAGCAACGGTAACTGA+TGG 0.574912 7_3:+32995196 None:intergenic
ACTGAGACAGAAGATGAAGC+TGG 0.588472 7_3:+32995592 None:intergenic
TCAGTGTTCACCTCTACCAT+AGG 0.593903 7_3:+32995283 None:intergenic
ATTTGCTAGATCAGACAAAG+AGG 0.602885 7_3:+32995459 None:intergenic
TCTGATCTAGCAAATTACAA+TGG 0.603309 7_3:-32995451 Msa1101880:CDS
GTACAGCAGAAACAAGGGGC+TGG 0.611388 7_3:-32996196 Msa1101880:five_prime_UTR
TGAGTAGAAGCTTTCAGCAA+CGG 0.612962 7_3:+32995186 None:intergenic
TGAATCACAAATGGTTAAGG+GGG 0.622020 7_3:-32995515 Msa1101880:CDS
CCACCGTACAGCAGAAACAA+GGG 0.625737 7_3:-32996201 None:intergenic
TCCGGAGACCAAAACTGAAG+AGG 0.627183 7_3:-32995893 Msa1101880:CDS
TAAACCCTAATCTTACTGGA+AGG 0.636292 7_3:+32996031 None:intergenic
CACCGTACAGCAGAAACAAG+GGG 0.664841 7_3:-32996200 None:intergenic
AGATTATAAAATTATAACAG+TGG 0.667391 7_3:-32995761 Msa1101880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATAAGTTTTTACATAT+TGG - chr7_3:32995376-32995395 Msa1101880:CDS 10.0%
!!! AAATATAAGTTTTTACATAT+TGG - chr7_3:32995376-32995395 Msa1101880:CDS 10.0%
!! AGATTATAAAATTATAACAG+TGG - chr7_3:32995276-32995295 Msa1101880:CDS 15.0%
!! AGATTATAAAATTATAACAG+TGG - chr7_3:32995276-32995295 Msa1101880:CDS 15.0%
!!! TCTTTATCAAGTTCTTTTTT+TGG - chr7_3:32994881-32994900 Msa1101880:three_prime_UTR 20.0%
!!! TAAGTTTTTACATATTGGAT+TGG - chr7_3:32995381-32995400 Msa1101880:CDS 20.0%
!!! TTATCTCTTTAAAAATCCTA+TGG - chr7_3:32995738-32995757 Msa1101880:CDS 20.0%
!!! CTTTTTTGTTTTTGTTTTTG+TGG - chr7_3:32995971-32995990 Msa1101880:intron 20.0%
!!! TCTTTATCAAGTTCTTTTTT+TGG - chr7_3:32994881-32994900 Msa1101880:three_prime_UTR 20.0%
!!! TAAGTTTTTACATATTGGAT+TGG - chr7_3:32995381-32995400 Msa1101880:CDS 20.0%
!!! TTATCTCTTTAAAAATCCTA+TGG - chr7_3:32995738-32995757 Msa1101880:CDS 20.0%
!!! CTTTTTTGTTTTTGTTTTTG+TGG - chr7_3:32995971-32995990 Msa1101880:intron 20.0%
! AAGAACTTGATAAAGAAAAC+AGG + chr7_3:32994877-32994896 None:intergenic 25.0%
! AAAAATCAGCTTGTAAGATA+TGG + chr7_3:32994925-32994944 None:intergenic 25.0%
!!! TTTTTTCCTTCATAAAAGCT+TGG - chr7_3:32994942-32994961 Msa1101880:three_prime_UTR 25.0%
! ATTTATTACGATCAAGAACA+GGG + chr7_3:32995069-32995088 None:intergenic 25.0%
! AATTTATTACGATCAAGAAC+AGG + chr7_3:32995070-32995089 None:intergenic 25.0%
!! TTTAATTTAGCAGATCTCAT+TGG - chr7_3:32995093-32995112 Msa1101880:three_prime_UTR 25.0%
!!! TATTTTTTTTATGCTACGGT+TGG + chr7_3:32995203-32995222 None:intergenic 25.0%
!!! TGGGTATTTTTTTTATGCTA+CGG + chr7_3:32995207-32995226 None:intergenic 25.0%
! AGCATAAAAAAAATACCCAT+CGG - chr7_3:32995208-32995227 Msa1101880:three_prime_UTR 25.0%
!! TTTTGAATCACAAATGGTTA+AGG - chr7_3:32995519-32995538 Msa1101880:CDS 25.0%
! TTTGAATCACAAATGGTTAA+GGG - chr7_3:32995520-32995539 Msa1101880:CDS 25.0%
!! GTGTTTTCAAATTCAAAGTT+AGG - chr7_3:32995671-32995690 Msa1101880:CDS 25.0%
! TCAAATTCAAAGTTAGGTAT+AGG - chr7_3:32995677-32995696 Msa1101880:CDS 25.0%
!!! GGATTTTTAAAGAGATAATC+CGG + chr7_3:32995736-32995755 None:intergenic 25.0%
! AATCTAATTATCAGAGCTAT+AGG - chr7_3:32995801-32995820 Msa1101880:CDS 25.0%
! TGTATTATGTCCAGTAATTA+TGG - chr7_3:32996003-32996022 Msa1101880:intron 25.0%
! AATTCCAAAACCATAATTAC+TGG + chr7_3:32996016-32996035 None:intergenic 25.0%
!!! GTTTTGGAATTGATAAATTC+TGG - chr7_3:32996025-32996044 Msa1101880:five_prime_UTR 25.0%
! AAGAACTTGATAAAGAAAAC+AGG + chr7_3:32994877-32994896 None:intergenic 25.0%
! AAAAATCAGCTTGTAAGATA+TGG + chr7_3:32994925-32994944 None:intergenic 25.0%
!!! TTTTTTCCTTCATAAAAGCT+TGG - chr7_3:32994942-32994961 Msa1101880:three_prime_UTR 25.0%
! ATTTATTACGATCAAGAACA+GGG + chr7_3:32995069-32995088 None:intergenic 25.0%
! AATTTATTACGATCAAGAAC+AGG + chr7_3:32995070-32995089 None:intergenic 25.0%
!! TTTAATTTAGCAGATCTCAT+TGG - chr7_3:32995093-32995112 Msa1101880:three_prime_UTR 25.0%
!!! TATTTTTTTTATGCTACGGT+TGG + chr7_3:32995203-32995222 None:intergenic 25.0%
!!! TGGGTATTTTTTTTATGCTA+CGG + chr7_3:32995207-32995226 None:intergenic 25.0%
! AGCATAAAAAAAATACCCAT+CGG - chr7_3:32995208-32995227 Msa1101880:three_prime_UTR 25.0%
!! TTTTGAATCACAAATGGTTA+AGG - chr7_3:32995519-32995538 Msa1101880:CDS 25.0%
! TTTGAATCACAAATGGTTAA+GGG - chr7_3:32995520-32995539 Msa1101880:CDS 25.0%
!! GTGTTTTCAAATTCAAAGTT+AGG - chr7_3:32995671-32995690 Msa1101880:CDS 25.0%
! TCAAATTCAAAGTTAGGTAT+AGG - chr7_3:32995677-32995696 Msa1101880:CDS 25.0%
!!! GGATTTTTAAAGAGATAATC+CGG + chr7_3:32995736-32995755 None:intergenic 25.0%
! AATCTAATTATCAGAGCTAT+AGG - chr7_3:32995801-32995820 Msa1101880:CDS 25.0%
! TGTATTATGTCCAGTAATTA+TGG - chr7_3:32996003-32996022 Msa1101880:intron 25.0%
! AATTCCAAAACCATAATTAC+TGG + chr7_3:32996016-32996035 None:intergenic 25.0%
!!! GTTTTGGAATTGATAAATTC+TGG - chr7_3:32996025-32996044 Msa1101880:five_prime_UTR 25.0%
GAGAATTGTAAATGCTTACT+TGG + chr7_3:32995035-32995054 None:intergenic 30.0%
TGCTTATAAAAGTTACCGAT+GGG + chr7_3:32995226-32995245 None:intergenic 30.0%
TTGCTTATAAAAGTTACCGA+TGG + chr7_3:32995227-32995246 None:intergenic 30.0%
! TAATCTTGTCTGAAGTTGAA+TGG + chr7_3:32995262-32995281 None:intergenic 30.0%
!!! GGTAAGTTTTGAATCACAAA+TGG - chr7_3:32995513-32995532 Msa1101880:CDS 30.0%
TTGAATCACAAATGGTTAAG+GGG - chr7_3:32995521-32995540 Msa1101880:CDS 30.0%
GACAAAGAGGTTGAAAAATT+TGG + chr7_3:32995568-32995587 None:intergenic 30.0%
TCTGATCTAGCAAATTACAA+TGG - chr7_3:32995586-32995605 Msa1101880:CDS 30.0%
TCTAGCAAATTACAATGGTT+TGG - chr7_3:32995591-32995610 Msa1101880:CDS 30.0%
!! ACACTTCTCTTTGAGTTTTT+GGG + chr7_3:32995633-32995652 None:intergenic 30.0%
AATGAAGACTTCTCTTAAAC+CGG - chr7_3:32995714-32995733 Msa1101880:CDS 30.0%
! CTTTAAAAATCCTATGGTAG+AGG - chr7_3:32995744-32995763 Msa1101880:CDS 30.0%
! AGCTCTGATAATTAGATTTG+AGG + chr7_3:32995799-32995818 None:intergenic 30.0%
AGCTATAGGTGTAAAATGAA+TGG - chr7_3:32995815-32995834 Msa1101880:CDS 30.0%
! AAGAAAGAGAATGAAGCATT+TGG + chr7_3:32995897-32995916 None:intergenic 30.0%
ATTCTCTTTCTTAGATCTTC+TGG - chr7_3:32995905-32995924 Msa1101880:CDS 30.0%
! ATGTCCAGTAATTATGGTTT+TGG - chr7_3:32996009-32996028 Msa1101880:intron 30.0%
GAGAATTGTAAATGCTTACT+TGG + chr7_3:32995035-32995054 None:intergenic 30.0%
TGCTTATAAAAGTTACCGAT+GGG + chr7_3:32995226-32995245 None:intergenic 30.0%
TTGCTTATAAAAGTTACCGA+TGG + chr7_3:32995227-32995246 None:intergenic 30.0%
! TAATCTTGTCTGAAGTTGAA+TGG + chr7_3:32995262-32995281 None:intergenic 30.0%
!!! GGTAAGTTTTGAATCACAAA+TGG - chr7_3:32995513-32995532 Msa1101880:CDS 30.0%
TTGAATCACAAATGGTTAAG+GGG - chr7_3:32995521-32995540 Msa1101880:CDS 30.0%
GACAAAGAGGTTGAAAAATT+TGG + chr7_3:32995568-32995587 None:intergenic 30.0%
TCTGATCTAGCAAATTACAA+TGG - chr7_3:32995586-32995605 Msa1101880:CDS 30.0%
TCTAGCAAATTACAATGGTT+TGG - chr7_3:32995591-32995610 Msa1101880:CDS 30.0%
!! ACACTTCTCTTTGAGTTTTT+GGG + chr7_3:32995633-32995652 None:intergenic 30.0%
AATGAAGACTTCTCTTAAAC+CGG - chr7_3:32995714-32995733 Msa1101880:CDS 30.0%
! CTTTAAAAATCCTATGGTAG+AGG - chr7_3:32995744-32995763 Msa1101880:CDS 30.0%
! AGCTCTGATAATTAGATTTG+AGG + chr7_3:32995799-32995818 None:intergenic 30.0%
AGCTATAGGTGTAAAATGAA+TGG - chr7_3:32995815-32995834 Msa1101880:CDS 30.0%
! AAGAAAGAGAATGAAGCATT+TGG + chr7_3:32995897-32995916 None:intergenic 30.0%
ATTCTCTTTCTTAGATCTTC+TGG - chr7_3:32995905-32995924 Msa1101880:CDS 30.0%
! ATGTCCAGTAATTATGGTTT+TGG - chr7_3:32996009-32996028 Msa1101880:intron 30.0%
! AGAGATCCAAGCTTTTATGA+AGG + chr7_3:32994951-32994970 None:intergenic 35.0%
TAAACCCTAATCTTACTGGA+AGG + chr7_3:32995009-32995028 None:intergenic 35.0%
GAGATAAACCCTAATCTTAC+TGG + chr7_3:32995013-32995032 None:intergenic 35.0%
! GCTATATTCCTCTTCAGTTT+TGG + chr7_3:32995155-32995174 None:intergenic 35.0%
!!! TTTTTTTATGCTACGGTTGG+TGG + chr7_3:32995200-32995219 None:intergenic 35.0%
! ATTGGTGCAATTTTCCATGA+TGG - chr7_3:32995399-32995418 Msa1101880:CDS 35.0%
AGATAGAGTCTTGAAGACTT+TGG + chr7_3:32995492-32995511 None:intergenic 35.0%
AAGTCTTCAAGACTCTATCT+TGG - chr7_3:32995492-32995511 Msa1101880:CDS 35.0%
TGAATCACAAATGGTTAAGG+GGG - chr7_3:32995522-32995541 Msa1101880:CDS 35.0%
! ATTTGCTAGATCAGACAAAG+AGG + chr7_3:32995581-32995600 None:intergenic 35.0%
! AGCAAATTACAATGGTTTGG+TGG - chr7_3:32995594-32995613 Msa1101880:CDS 35.0%
!! ACTTCTCTTTGAGTTTTTGG+GGG + chr7_3:32995631-32995650 None:intergenic 35.0%
!! CACTTCTCTTTGAGTTTTTG+GGG + chr7_3:32995632-32995651 None:intergenic 35.0%
!! CACACTTCTCTTTGAGTTTT+TGG + chr7_3:32995634-32995653 None:intergenic 35.0%
! TTCTTAGATCTTCTGGTTGT+TGG - chr7_3:32995912-32995931 Msa1101880:CDS 35.0%
! TCTTAGATCTTCTGGTTGTT+GGG - chr7_3:32995913-32995932 Msa1101880:CDS 35.0%
TACTTTCGTATGCTTTGTGT+TGG - chr7_3:32996101-32996120 Msa1101880:five_prime_UTR 35.0%
! AGAGATCCAAGCTTTTATGA+AGG + chr7_3:32994951-32994970 None:intergenic 35.0%
TAAACCCTAATCTTACTGGA+AGG + chr7_3:32995009-32995028 None:intergenic 35.0%
GAGATAAACCCTAATCTTAC+TGG + chr7_3:32995013-32995032 None:intergenic 35.0%
! GCTATATTCCTCTTCAGTTT+TGG + chr7_3:32995155-32995174 None:intergenic 35.0%
!!! TTTTTTTATGCTACGGTTGG+TGG + chr7_3:32995200-32995219 None:intergenic 35.0%
! ATTGGTGCAATTTTCCATGA+TGG - chr7_3:32995399-32995418 Msa1101880:CDS 35.0%
AGATAGAGTCTTGAAGACTT+TGG + chr7_3:32995492-32995511 None:intergenic 35.0%
AAGTCTTCAAGACTCTATCT+TGG - chr7_3:32995492-32995511 Msa1101880:CDS 35.0%
TGAATCACAAATGGTTAAGG+GGG - chr7_3:32995522-32995541 Msa1101880:CDS 35.0%
! ATTTGCTAGATCAGACAAAG+AGG + chr7_3:32995581-32995600 None:intergenic 35.0%
! AGCAAATTACAATGGTTTGG+TGG - chr7_3:32995594-32995613 Msa1101880:CDS 35.0%
!! ACTTCTCTTTGAGTTTTTGG+GGG + chr7_3:32995631-32995650 None:intergenic 35.0%
!! CACTTCTCTTTGAGTTTTTG+GGG + chr7_3:32995632-32995651 None:intergenic 35.0%
!! CACACTTCTCTTTGAGTTTT+TGG + chr7_3:32995634-32995653 None:intergenic 35.0%
! TTCTTAGATCTTCTGGTTGT+TGG - chr7_3:32995912-32995931 Msa1101880:CDS 35.0%
! TCTTAGATCTTCTGGTTGTT+GGG - chr7_3:32995913-32995932 Msa1101880:CDS 35.0%
TACTTTCGTATGCTTTGTGT+TGG - chr7_3:32996101-32996120 Msa1101880:five_prime_UTR 35.0%
! GCAGATCTCATTGGTGATAA+TGG - chr7_3:32995102-32995121 Msa1101880:three_prime_UTR 40.0%
! AGCTTTATCTCTCGTTGATC+CGG - chr7_3:32995126-32995145 Msa1101880:three_prime_UTR 40.0%
!!! GCAATTTTCCATGATGGATC+AGG - chr7_3:32995405-32995424 Msa1101880:CDS 40.0%
ATCATGATCCTGATCCATCA+TGG + chr7_3:32995416-32995435 None:intergenic 40.0%
AGGGGGCAGTAAAGTTTAAT+TGG - chr7_3:32995539-32995558 Msa1101880:CDS 40.0%
TGAGTAGAAGCTTTCAGCAA+CGG + chr7_3:32995854-32995873 None:intergenic 40.0%
!! AGAATGAAGCATTTGGCTTG+TGG + chr7_3:32995890-32995909 None:intergenic 40.0%
ATTCGTGCTTGTAAACTCAC+TGG - chr7_3:32996150-32996169 Msa1101880:five_prime_UTR 40.0%
! GCAGATCTCATTGGTGATAA+TGG - chr7_3:32995102-32995121 Msa1101880:three_prime_UTR 40.0%
! AGCTTTATCTCTCGTTGATC+CGG - chr7_3:32995126-32995145 Msa1101880:three_prime_UTR 40.0%
!!! GCAATTTTCCATGATGGATC+AGG - chr7_3:32995405-32995424 Msa1101880:CDS 40.0%
ATCATGATCCTGATCCATCA+TGG + chr7_3:32995416-32995435 None:intergenic 40.0%
AGGGGGCAGTAAAGTTTAAT+TGG - chr7_3:32995539-32995558 Msa1101880:CDS 40.0%
TGAGTAGAAGCTTTCAGCAA+CGG + chr7_3:32995854-32995873 None:intergenic 40.0%
!! AGAATGAAGCATTTGGCTTG+TGG + chr7_3:32995890-32995909 None:intergenic 40.0%
ATTCGTGCTTGTAAACTCAC+TGG - chr7_3:32996150-32996169 Msa1101880:five_prime_UTR 40.0%
! GGCAGGAAGGTTTTTCTTCT+TGG + chr7_3:32994988-32995007 None:intergenic 45.0%
AATCTTACTGGAAGGCAGGA+AGG + chr7_3:32995001-32995020 None:intergenic 45.0%
TCCTGCCTTCCAGTAAGATT+AGG - chr7_3:32995001-32995020 Msa1101880:three_prime_UTR 45.0%
CCTGCCTTCCAGTAAGATTA+GGG - chr7_3:32995002-32995021 Msa1101880:three_prime_UTR 45.0%
!! TCCTCTTCAGTTTTGGTCTC+CGG + chr7_3:32995148-32995167 None:intergenic 45.0%
!! TACGGTTGGTGGTCTTATAC+TGG + chr7_3:32995189-32995208 None:intergenic 45.0%
ACTGAGACAGAAGATGAAGC+TGG + chr7_3:32995448-32995467 None:intergenic 45.0%
TCAGTGTTCACCTCTACCAT+AGG + chr7_3:32995757-32995776 None:intergenic 45.0%
CTTTCAGCAACGGTAACTGA+TGG + chr7_3:32995844-32995863 None:intergenic 45.0%
! GGCAGGAAGGTTTTTCTTCT+TGG + chr7_3:32994988-32995007 None:intergenic 45.0%
AATCTTACTGGAAGGCAGGA+AGG + chr7_3:32995001-32995020 None:intergenic 45.0%
TCCTGCCTTCCAGTAAGATT+AGG - chr7_3:32995001-32995020 Msa1101880:three_prime_UTR 45.0%
CCTGCCTTCCAGTAAGATTA+GGG - chr7_3:32995002-32995021 Msa1101880:three_prime_UTR 45.0%
!! TCCTCTTCAGTTTTGGTCTC+CGG + chr7_3:32995148-32995167 None:intergenic 45.0%
!! TACGGTTGGTGGTCTTATAC+TGG + chr7_3:32995189-32995208 None:intergenic 45.0%
ACTGAGACAGAAGATGAAGC+TGG + chr7_3:32995448-32995467 None:intergenic 45.0%
TCAGTGTTCACCTCTACCAT+AGG + chr7_3:32995757-32995776 None:intergenic 45.0%
CTTTCAGCAACGGTAACTGA+TGG + chr7_3:32995844-32995863 None:intergenic 45.0%
!!! AGAAACAAGGGGCTGGCTTT+TGG - chr7_3:32994848-32994867 Msa1101880:three_prime_UTR 50.0%
CCCTAATCTTACTGGAAGGC+AGG + chr7_3:32995005-32995024 None:intergenic 50.0%
TCCGGAGACCAAAACTGAAG+AGG - chr7_3:32995144-32995163 Msa1101880:three_prime_UTR 50.0%
!!! AGAAACAAGGGGCTGGCTTT+TGG - chr7_3:32994848-32994867 Msa1101880:three_prime_UTR 50.0%
CCCTAATCTTACTGGAAGGC+AGG + chr7_3:32995005-32995024 None:intergenic 50.0%
TCCGGAGACCAAAACTGAAG+AGG - chr7_3:32995144-32995163 Msa1101880:three_prime_UTR 50.0%
GTACAGCAGAAACAAGGGGC+TGG - chr7_3:32994841-32994860 Msa1101880:three_prime_UTR 55.0%
GTACAGCAGAAACAAGGGGC+TGG - chr7_3:32994841-32994860 Msa1101880:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 32994841 32996218 32994841 ID=Msa1101880;Name=Msa1101880
chr7_3 mRNA 32994841 32996218 32994841 ID=Msa1101880-mRNA-1;Parent=Msa1101880;Name=Msa1101880-mRNA-1;_AED=0.00;_eAED=0.00;_QI=210|1|0.5|1|0|0|2|411|228
chr7_3 exon 32994841 32995953 32994841 ID=Msa1101880-mRNA-1:exon:7154;Parent=Msa1101880-mRNA-1
chr7_3 exon 32996024 32996218 32996024 ID=Msa1101880-mRNA-1:exon:7153;Parent=Msa1101880-mRNA-1
chr7_3 five_prime_UTR 32996024 32996218 32996024 ID=Msa1101880-mRNA-1:five_prime_utr;Parent=Msa1101880-mRNA-1
chr7_3 five_prime_UTR 32995939 32995953 32995939 ID=Msa1101880-mRNA-1:five_prime_utr;Parent=Msa1101880-mRNA-1
chr7_3 CDS 32995252 32995938 32995252 ID=Msa1101880-mRNA-1:cds;Parent=Msa1101880-mRNA-1
chr7_3 three_prime_UTR 32994841 32995251 32994841 ID=Msa1101880-mRNA-1:three_prime_utr;Parent=Msa1101880-mRNA-1
Gene Sequence

>Msa1101880

ATGGCAGCTTTATCTCTCGTTGATCCGGAGACCAAAACTGAAGAGGAATATAGCATAGTTCACTTCCAGTATAAGACCACCAACCGTAGCATAAAAAAAATACCCATCGGTAACTTTTATAAGCAAACACTACTTTCTCCATTCAACTTCAGACAAGATTATAAAATTATAACAGTGGAGAAAAATCTTTCTTTGAAAAACGAAAGCAAAGACATATATTTGTTATCTAGAGATGACATTAGAAGTTATCATAGCAAATATAAGTTTTTACATATTGGATTGGTGCAATTTTCCATGATGGATCAGGATCATGATTATTATGAACCAGCTTCATCTTCTGTCTCAGTTAGATTGAGAGACTCAAAGTACCAAAGTCTTCAAGACTCTATCTTGGTAAGTTTTGAATCACAAATGGTTAAGGGGGCAGTAAAGTTTAATTGGTTTCCAAATTTTTCAACCTCTTTGTCTGATCTAGCAAATTACAATGGTTTGGTGGTTACTATAGAACCCCCAAAAACTCAAAGAGAAGTGTGCATTAATAATAAATTACGTGTTTTCAAATTCAAAGTTAGGTATAGGATGTGTTACAAATTAATGAAGACTTCTCTTAAACCGGATTATCTCTTTAAAAATCCTATGGTAGAGGTGAACACTGAGATCACAAACTTTGTTGTTCCTCAAATCTAA
Protein sequence

>Msa1101880

MAALSLVDPETKTEEEYSIVHFQYKTTNRSIKKIPIGNFYKQTLLSPFNFRQDYKIITVEKNLSLKNESKDIYLLSRDDIRSYHSKYKFLHIGLVQFSMMDQDHDYYEPASSSVSVRLRDSKYQSLQDSILVSFESQMVKGAVKFNWFPNFSTSLSDLANYNGLVVTIEPPKTQREVCINNKLRVFKFKVRYRMCYKLMKTSLKPDYLFKNPMVEVNTEITNFVVPQI*