AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0257320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0257320 MtrunA17_Chr2g0319291 98.361 122 2 0 1 122 1 122 5.07e-88 251
Msa0257320 MtrunA17_Chr4g0042051 72.131 122 34 0 1 122 1 122 1.04e-64 192
Msa0257320 MtrunA17_Chr2g0280411 61.607 112 43 0 9 120 8 119 1.48e-49 154
Msa0257320 MtrunA17_Chr8g0334481 39.394 99 60 0 15 113 31 129 7.86e-24 89.4
Msa0257320 MtrunA17_Chr7g0245141 36.364 110 70 0 12 121 26 135 1.57e-23 88.6
Msa0257320 MtrunA17_Chr7g0216481 39.175 97 59 0 17 113 33 129 2.18e-23 87.8
Msa0257320 MtrunA17_Chr5g0404831 34.513 113 74 0 8 120 6 118 2.40e-23 87.4
Msa0257320 MtrunA17_Chr1g0155721 34.513 113 74 0 8 120 4 116 5.41e-22 84.0
Msa0257320 MtrunA17_Chr3g0142051 34.906 106 69 0 8 113 4 109 8.42e-22 83.6
Msa0257320 MtrunA17_Chr8g0393201 34.579 107 70 0 7 113 224 330 1.96e-20 84.3
Msa0257320 MtrunA17_Chr5g0415771 34.286 105 69 0 10 114 19 123 2.63e-16 69.7
Msa0257320 MtrunA17_Chr5g0415761 33.333 105 70 0 10 114 20 124 1.45e-14 65.1
Msa0257320 MtrunA17_Chr5g0415741 32.381 105 71 0 10 114 18 122 4.79e-13 61.2
Msa0257320 MtrunA17_Chr4g0047431 33.708 89 59 0 34 122 23 111 5.96e-12 61.2
Msa0257320 MtrunA17_Chr4g0052481 25.000 108 75 2 13 118 62 165 6.75e-12 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0257320 AT1G11530.1 63.063 111 41 0 10 120 2 112 4.83e-53 162
Msa0257320 AT3G08710.2 42.056 107 62 0 15 121 28 134 1.45e-25 94.0
Msa0257320 AT3G08710.3 42.056 107 62 0 15 121 28 134 1.45e-25 94.0
Msa0257320 AT3G08710.1 42.056 107 62 0 15 121 28 134 1.45e-25 94.0
Msa0257320 AT3G56420.1 40.000 105 63 0 9 113 39 143 1.59e-24 91.7
Msa0257320 AT3G56420.2 40.000 105 63 0 9 113 39 143 1.59e-24 91.7
Msa0257320 AT3G56420.3 40.000 105 63 0 9 113 39 143 1.59e-24 91.7
Msa0257320 AT3G51030.1 39.623 106 64 0 8 113 4 109 1.85e-24 90.5
Msa0257320 AT2G40790.2 37.963 108 67 0 6 113 36 143 1.88e-23 89.0
Msa0257320 AT2G40790.1 37.963 108 67 0 6 113 36 143 1.88e-23 89.0
Msa0257320 AT1G45145.1 32.381 105 71 0 9 113 4 108 1.82e-21 82.8
Msa0257320 AT1G59730.1 31.731 104 71 0 12 115 23 126 2.71e-21 82.8
Msa0257320 AT5G39950.1 34.615 104 68 0 10 113 25 128 1.20e-20 81.3
Msa0257320 AT1G19730.1 30.841 107 73 1 8 113 4 110 4.67e-19 76.6
Msa0257320 AT5G42980.1 32.673 101 68 0 9 109 4 104 2.00e-18 75.1
Msa0257320 AT3G17880.2 32.143 112 76 0 2 113 255 366 5.13e-18 78.6
Msa0257320 AT3G17880.1 32.143 112 76 0 2 113 262 373 5.87e-18 78.2
Msa0257320 AT1G69880.1 27.778 108 78 0 12 119 39 146 8.32e-17 71.6
Msa0257320 AT2G35010.2 30.841 107 72 1 12 116 86 192 7.45e-14 65.1
Msa0257320 AT2G35010.1 30.841 107 72 1 12 116 86 192 7.45e-14 65.1
Msa0257320 AT4G04950.1 34.831 89 58 0 34 122 23 111 4.31e-13 64.7
Msa0257320 AT1G43560.1 27.523 109 77 2 12 118 56 164 9.83e-13 61.6
Msa0257320 AT4G32580.1 33.333 90 59 1 34 122 23 112 1.50e-12 60.8
Msa0257320 AT1G31020.1 31.313 99 66 1 9 105 48 146 3.23e-12 60.1
Msa0257320 AT4G03520.1 30.841 107 69 3 13 117 82 185 2.09e-11 58.5

Find 37 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTCCATAATAGATCTTT+AGG 0.279018 2_2:-53059399 None:intergenic
TACTGGTTGTTGTGTGTGTT+TGG 0.281902 2_2:+53059483 Msa0257320:three_prime_UTR
TCCTTCCTAAAGATCTATTA+TGG 0.287061 2_2:+53059394 Msa0257320:exon
GAATCCTTTCTTTCAAGAAT+TGG 0.317619 2_2:+53058307 Msa0257320:CDS
TTGATAAAGTTATAAAAGAT+AGG 0.352325 2_2:-53059572 None:intergenic
TATGGCTTTAATTTCCATCT+TGG 0.365887 2_2:-53059270 None:intergenic
GATGCCAATTCTTGAAAGAA+AGG 0.374604 2_2:-53058311 None:intergenic
GAGTATTAAATGAGAAATAC+TGG 0.379960 2_2:-53059549 None:intergenic
GTGCAAATCCTGATGAAATA+AGG 0.381132 2_2:+53059342 Msa0257320:CDS
ACTGGTTGTTGTGTGTGTTT+GGG 0.390313 2_2:+53059484 Msa0257320:three_prime_UTR
ATAGTCTAGAACTATAGTTT+TGG 0.402215 2_2:+53059457 Msa0257320:three_prime_UTR
ATTGGATGTGGATGAGGTTA+AGG 0.406643 2_2:+53058361 Msa0257320:CDS
ACTTACGGGGTAACTTTGAT+TGG 0.412403 2_2:-53057977 None:intergenic
ACAACAACAAAAGAAAGAAA+TGG 0.413741 2_2:+53057873 Msa0257320:exon
GAAAGGATTCATTTGTATAG+AGG 0.416952 2_2:-53058294 None:intergenic
TTTCTTTGTGTAAATATTTG+TGG 0.422046 2_2:+53059776 Msa0257320:three_prime_UTR
TCTCTTTCTCACATTGGATG+TGG 0.439009 2_2:+53058349 Msa0257320:CDS
GGCTTTAATTTCCATCTTGG+AGG 0.439612 2_2:-53059267 None:intergenic
ATCCTGATGAAATAAGGAAA+AGG 0.449385 2_2:+53059348 Msa0257320:CDS
AAAACTACTTATGTACTGTA+TGG 0.474906 2_2:+53059681 Msa0257320:three_prime_UTR
TCTCACATTGGATGTGGATG+AGG 0.476730 2_2:+53058355 Msa0257320:CDS
CTTGAATGGTGGAACTCTTG+TGG 0.483614 2_2:+53059307 Msa0257320:CDS
AAAGGATTCATTTGTATAGA+GGG 0.495640 2_2:-53058293 None:intergenic
TCCTGATGAAATAAGGAAAA+GGG 0.497571 2_2:+53059349 Msa0257320:CDS
AGATGTTCTCTTTCTCACAT+TGG 0.507095 2_2:+53058343 Msa0257320:CDS
CAAGAAAAGAAAGGTAGGTA+TGG 0.508139 2_2:-53059288 None:intergenic
TGATAAAGTTATAAAAGATA+GGG 0.526654 2_2:-53059571 None:intergenic
TCCATAATAGATCTTTAGGA+AGG 0.541604 2_2:-53059395 None:intergenic
TCAGGAAATTGCCTCCAAGA+TGG 0.545444 2_2:+53059256 Msa0257320:intron
TCCACCATTCAAGAAAAGAA+AGG 0.564993 2_2:-53059297 None:intergenic
ACAACAAAAGAAAGAAATGG+AGG 0.577149 2_2:+53057876 Msa0257320:exon
AACTCTTGTGGACAAAATTG+TGG 0.579160 2_2:+53059319 Msa0257320:CDS
CAACAAAAGAAAGAAATGGA+GGG 0.588366 2_2:+53057877 Msa0257320:exon
CCATTCAAGAAAAGAAAGGT+AGG 0.610326 2_2:-53059293 None:intergenic
ACTCTTGTGGACAAAATTGT+GGG 0.617397 2_2:+53059320 Msa0257320:CDS
AAACACACACAACAACCAGT+AGG 0.679842 2_2:-53059481 None:intergenic
TGGAAACAATGAAATCAACA+AGG 0.716165 2_2:+53059414 Msa0257320:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATCAAGTTTTTTTTTTT+AGG + chr2_2:53059587-53059606 Msa0257320:three_prime_UTR 10.0%
!!! TTATCAAGTTTTTTTTTTTA+GGG + chr2_2:53059588-53059607 Msa0257320:three_prime_UTR 10.0%
!!! AGTAGTTTTTTTTTTTTTTA+AGG - chr2_2:53059670-53059689 None:intergenic 10.0%
!!! TTTATCAAGTTTTTTTTTTT+AGG + chr2_2:53059587-53059606 Msa0257320:three_prime_UTR 10.0%
!!! TTATCAAGTTTTTTTTTTTA+GGG + chr2_2:53059588-53059607 Msa0257320:three_prime_UTR 10.0%
!!! AGTAGTTTTTTTTTTTTTTA+AGG - chr2_2:53059670-53059689 None:intergenic 10.0%
!!! AATTTGGAGTAAAAAATAAA+AGG - chr2_2:53058790-53058809 None:intergenic 15.0%
!!! TTTTTTTTGACAAAAATAGT+TGG - chr2_2:53058829-53058848 None:intergenic 15.0%
!! TTTGTAGGATAATAAATTAT+AGG + chr2_2:53058874-53058893 Msa0257320:intron 15.0%
!! TGATAAAGTTATAAAAGATA+GGG - chr2_2:53059574-53059593 None:intergenic 15.0%
!! TTGATAAAGTTATAAAAGAT+AGG - chr2_2:53059575-53059594 None:intergenic 15.0%
!!! AACTATATCATGTTTTTATT+TGG + chr2_2:53059611-53059630 Msa0257320:three_prime_UTR 15.0%
!! AAAAACTAAATAATCATATG+TGG + chr2_2:53059808-53059827 Msa0257320:three_prime_UTR 15.0%
!!! AATTTGGAGTAAAAAATAAA+AGG - chr2_2:53058790-53058809 None:intergenic 15.0%
!!! TTTTTTTTGACAAAAATAGT+TGG - chr2_2:53058829-53058848 None:intergenic 15.0%
!! TTTGTAGGATAATAAATTAT+AGG + chr2_2:53058874-53058893 Msa0257320:intron 15.0%
!! TGATAAAGTTATAAAAGATA+GGG - chr2_2:53059574-53059593 None:intergenic 15.0%
!! TTGATAAAGTTATAAAAGAT+AGG - chr2_2:53059575-53059594 None:intergenic 15.0%
!!! AACTATATCATGTTTTTATT+TGG + chr2_2:53059611-53059630 Msa0257320:three_prime_UTR 15.0%
!! AAAATATCTCAAAAAGTCTA+AGG + chr2_2:53057903-53057922 Msa0257320:CDS 20.0%
!! AAATAGACTCAAAAAAATCA+TGG + chr2_2:53057932-53057951 Msa0257320:CDS 20.0%
!! AATAGACTCAAAAAAATCAT+GGG + chr2_2:53057933-53057952 Msa0257320:CDS 20.0%
!! CTAGATATGTAACAAAATTA+CGG - chr2_2:53058079-53058098 None:intergenic 20.0%
!!! GTTACTCAAAAAACAAATTT+TGG - chr2_2:53058203-53058222 None:intergenic 20.0%
!!! AAATTTGTTTTTTGAGTAAC+CGG + chr2_2:53058203-53058222 Msa0257320:intron 20.0%
!!! ATTTGCTTAACTTTTAACAA+TGG + chr2_2:53058653-53058672 Msa0257320:intron 20.0%
!! TAAATTTCAAAATGTTACTG+AGG + chr2_2:53059009-53059028 Msa0257320:intron 20.0%
!!! TATTTTCCCTAACATTAATA+AGG - chr2_2:53059102-53059121 None:intergenic 20.0%
!! ATTGTGTACATTAATATATG+CGG + chr2_2:53059227-53059246 Msa0257320:intron 20.0%
!!! TATATCATGTTTTTATTTGG+TGG + chr2_2:53059614-53059633 Msa0257320:three_prime_UTR 20.0%
!!! ATATCATGTTTTTATTTGGT+GGG + chr2_2:53059615-53059634 Msa0257320:three_prime_UTR 20.0%
!! TTTCTTTGTGTAAATATTTG+TGG + chr2_2:53059776-53059795 Msa0257320:three_prime_UTR 20.0%
!! AAAATATCTCAAAAAGTCTA+AGG + chr2_2:53057903-53057922 Msa0257320:CDS 20.0%
!! AAATAGACTCAAAAAAATCA+TGG + chr2_2:53057932-53057951 Msa0257320:CDS 20.0%
!! AATAGACTCAAAAAAATCAT+GGG + chr2_2:53057933-53057952 Msa0257320:CDS 20.0%
!! CTAGATATGTAACAAAATTA+CGG - chr2_2:53058079-53058098 None:intergenic 20.0%
!!! GTTACTCAAAAAACAAATTT+TGG - chr2_2:53058203-53058222 None:intergenic 20.0%
!!! AAATTTGTTTTTTGAGTAAC+CGG + chr2_2:53058203-53058222 Msa0257320:intron 20.0%
!!! ATTTGCTTAACTTTTAACAA+TGG + chr2_2:53058653-53058672 Msa0257320:intron 20.0%
!! TAAATTTCAAAATGTTACTG+AGG + chr2_2:53059009-53059028 Msa0257320:intron 20.0%
!!! TATTTTCCCTAACATTAATA+AGG - chr2_2:53059102-53059121 None:intergenic 20.0%
!! ATTGTGTACATTAATATATG+CGG + chr2_2:53059227-53059246 Msa0257320:intron 20.0%
!!! TATATCATGTTTTTATTTGG+TGG + chr2_2:53059614-53059633 Msa0257320:three_prime_UTR 20.0%
!!! ATATCATGTTTTTATTTGGT+GGG + chr2_2:53059615-53059634 Msa0257320:three_prime_UTR 20.0%
!! TTTCTTTGTGTAAATATTTG+TGG + chr2_2:53059776-53059795 Msa0257320:three_prime_UTR 20.0%
! ACAACAACAAAAGAAAGAAA+TGG + chr2_2:53057873-53057892 Msa0257320:exon 25.0%
! TCATAAACAAAGAAGACTTA+CGG - chr2_2:53057995-53058014 None:intergenic 25.0%
!! TTTTGGTCTAAAAAAATTGC+AGG + chr2_2:53058238-53058257 Msa0257320:intron 25.0%
! AAAGGATTCATTTGTATAGA+GGG - chr2_2:53058296-53058315 None:intergenic 25.0%
! ATGGTATATAAAGGAAATCA+AGG + chr2_2:53058481-53058500 Msa0257320:intron 25.0%
! GAATATAGTATATGCAAACA+AGG - chr2_2:53058578-53058597 None:intergenic 25.0%
! AAGCATGAACTCATTTAATT+TGG - chr2_2:53058806-53058825 None:intergenic 25.0%
! AAAAAAATGCTTCCAACTAT+TGG + chr2_2:53058848-53058867 Msa0257320:intron 25.0%
! AAGTGAACCTTATTAATGTT+AGG + chr2_2:53059092-53059111 Msa0257320:intron 25.0%
! AGTGAACCTTATTAATGTTA+GGG + chr2_2:53059093-53059112 Msa0257320:intron 25.0%
! GTACACAATAATAGAAATAG+TGG - chr2_2:53059216-53059235 None:intergenic 25.0%
! TGTTTCCATAATAGATCTTT+AGG - chr2_2:53059402-53059421 None:intergenic 25.0%
!!! ATAGTCTAGAACTATAGTTT+TGG + chr2_2:53059457-53059476 Msa0257320:three_prime_UTR 25.0%
! GAGTATTAAATGAGAAATAC+TGG - chr2_2:53059552-53059571 None:intergenic 25.0%
! AAAACTACTTATGTACTGTA+TGG + chr2_2:53059681-53059700 Msa0257320:three_prime_UTR 25.0%
! ACAACAACAAAAGAAAGAAA+TGG + chr2_2:53057873-53057892 Msa0257320:exon 25.0%
! TCATAAACAAAGAAGACTTA+CGG - chr2_2:53057995-53058014 None:intergenic 25.0%
!! TTTTGGTCTAAAAAAATTGC+AGG + chr2_2:53058238-53058257 Msa0257320:intron 25.0%
! AAAGGATTCATTTGTATAGA+GGG - chr2_2:53058296-53058315 None:intergenic 25.0%
! ATGGTATATAAAGGAAATCA+AGG + chr2_2:53058481-53058500 Msa0257320:intron 25.0%
! GAATATAGTATATGCAAACA+AGG - chr2_2:53058578-53058597 None:intergenic 25.0%
! AAGCATGAACTCATTTAATT+TGG - chr2_2:53058806-53058825 None:intergenic 25.0%
! AAAAAAATGCTTCCAACTAT+TGG + chr2_2:53058848-53058867 Msa0257320:intron 25.0%
! AAGTGAACCTTATTAATGTT+AGG + chr2_2:53059092-53059111 Msa0257320:intron 25.0%
! AGTGAACCTTATTAATGTTA+GGG + chr2_2:53059093-53059112 Msa0257320:intron 25.0%
! GTACACAATAATAGAAATAG+TGG - chr2_2:53059216-53059235 None:intergenic 25.0%
! TGTTTCCATAATAGATCTTT+AGG - chr2_2:53059402-53059421 None:intergenic 25.0%
!!! ATAGTCTAGAACTATAGTTT+TGG + chr2_2:53059457-53059476 Msa0257320:three_prime_UTR 25.0%
! GAGTATTAAATGAGAAATAC+TGG - chr2_2:53059552-53059571 None:intergenic 25.0%
! AAAACTACTTATGTACTGTA+TGG + chr2_2:53059681-53059700 Msa0257320:three_prime_UTR 25.0%
ACAACAAAAGAAAGAAATGG+AGG + chr2_2:53057876-53057895 Msa0257320:exon 30.0%
CAACAAAAGAAAGAAATGGA+GGG + chr2_2:53057877-53057896 Msa0257320:exon 30.0%
ATAAACAAAGAAGACTTACG+GGG - chr2_2:53057993-53058012 None:intergenic 30.0%
CATAAACAAAGAAGACTTAC+GGG - chr2_2:53057994-53058013 None:intergenic 30.0%
TTGTTACATATCTAGACGAT+AGG + chr2_2:53058084-53058103 Msa0257320:intron 30.0%
TCTAAAAAAATTGCAGGTTG+TGG + chr2_2:53058244-53058263 Msa0257320:intron 30.0%
!! TGGTTCATTTTTCTGCTTTT+TGG + chr2_2:53058264-53058283 Msa0257320:CDS 30.0%
GAAAGGATTCATTTGTATAG+AGG - chr2_2:53058297-53058316 None:intergenic 30.0%
GAATCCTTTCTTTCAAGAAT+TGG + chr2_2:53058307-53058326 Msa0257320:CDS 30.0%
TATATACCATTTGCATGTCT+TGG - chr2_2:53058471-53058490 None:intergenic 30.0%
GACATGCAAATGGTATATAA+AGG + chr2_2:53058472-53058491 Msa0257320:intron 30.0%
! ACACTTTAGATGGATTTGTA+TGG - chr2_2:53058516-53058535 None:intergenic 30.0%
! CCATCTAAAGTGTTTGTATT+CGG + chr2_2:53058523-53058542 Msa0257320:intron 30.0%
ATATGATACATAGTGGTGTT+TGG - chr2_2:53058552-53058571 None:intergenic 30.0%
! TCCAACTATTGGTATTTTGT+AGG + chr2_2:53058859-53058878 Msa0257320:intron 30.0%
TCCTACAAAATACCAATAGT+TGG - chr2_2:53058863-53058882 None:intergenic 30.0%
ATGTTGTTACACTCGAAAAA+TGG - chr2_2:53059039-53059058 None:intergenic 30.0%
!! TATGGCTTTAATTTCCATCT+TGG - chr2_2:53059273-53059292 None:intergenic 30.0%
ATCCTGATGAAATAAGGAAA+AGG + chr2_2:53059348-53059367 Msa0257320:CDS 30.0%
TCCTGATGAAATAAGGAAAA+GGG + chr2_2:53059349-53059368 Msa0257320:CDS 30.0%
TCCTTCCTAAAGATCTATTA+TGG + chr2_2:53059394-53059413 Msa0257320:exon 30.0%
! TCCATAATAGATCTTTAGGA+AGG - chr2_2:53059398-53059417 None:intergenic 30.0%
TGGAAACAATGAAATCAACA+AGG + chr2_2:53059414-53059433 Msa0257320:three_prime_UTR 30.0%
ACAACAAAAGAAAGAAATGG+AGG + chr2_2:53057876-53057895 Msa0257320:exon 30.0%
CAACAAAAGAAAGAAATGGA+GGG + chr2_2:53057877-53057896 Msa0257320:exon 30.0%
ATAAACAAAGAAGACTTACG+GGG - chr2_2:53057993-53058012 None:intergenic 30.0%
CATAAACAAAGAAGACTTAC+GGG - chr2_2:53057994-53058013 None:intergenic 30.0%
TTGTTACATATCTAGACGAT+AGG + chr2_2:53058084-53058103 Msa0257320:intron 30.0%
TCTAAAAAAATTGCAGGTTG+TGG + chr2_2:53058244-53058263 Msa0257320:intron 30.0%
!! TGGTTCATTTTTCTGCTTTT+TGG + chr2_2:53058264-53058283 Msa0257320:CDS 30.0%
GAAAGGATTCATTTGTATAG+AGG - chr2_2:53058297-53058316 None:intergenic 30.0%
GAATCCTTTCTTTCAAGAAT+TGG + chr2_2:53058307-53058326 Msa0257320:CDS 30.0%
TATATACCATTTGCATGTCT+TGG - chr2_2:53058471-53058490 None:intergenic 30.0%
GACATGCAAATGGTATATAA+AGG + chr2_2:53058472-53058491 Msa0257320:intron 30.0%
! ACACTTTAGATGGATTTGTA+TGG - chr2_2:53058516-53058535 None:intergenic 30.0%
! CCATCTAAAGTGTTTGTATT+CGG + chr2_2:53058523-53058542 Msa0257320:intron 30.0%
ATATGATACATAGTGGTGTT+TGG - chr2_2:53058552-53058571 None:intergenic 30.0%
! TCCAACTATTGGTATTTTGT+AGG + chr2_2:53058859-53058878 Msa0257320:intron 30.0%
TCCTACAAAATACCAATAGT+TGG - chr2_2:53058863-53058882 None:intergenic 30.0%
ATGTTGTTACACTCGAAAAA+TGG - chr2_2:53059039-53059058 None:intergenic 30.0%
!! TATGGCTTTAATTTCCATCT+TGG - chr2_2:53059273-53059292 None:intergenic 30.0%
ATCCTGATGAAATAAGGAAA+AGG + chr2_2:53059348-53059367 Msa0257320:CDS 30.0%
TCCTGATGAAATAAGGAAAA+GGG + chr2_2:53059349-53059368 Msa0257320:CDS 30.0%
TCCTTCCTAAAGATCTATTA+TGG + chr2_2:53059394-53059413 Msa0257320:exon 30.0%
! TCCATAATAGATCTTTAGGA+AGG - chr2_2:53059398-53059417 None:intergenic 30.0%
TGGAAACAATGAAATCAACA+AGG + chr2_2:53059414-53059433 Msa0257320:three_prime_UTR 30.0%
ACCAAAATGATCTCTTAAGC+CGG - chr2_2:53058225-53058244 None:intergenic 35.0%
GATGCCAATTCTTGAAAGAA+AGG - chr2_2:53058314-53058333 None:intergenic 35.0%
AGATGTTCTCTTTCTCACAT+TGG + chr2_2:53058343-53058362 Msa0257320:CDS 35.0%
CCGAATACAAACACTTTAGA+TGG - chr2_2:53058526-53058545 None:intergenic 35.0%
! AAGGAGCATATGATACATAG+TGG - chr2_2:53058559-53058578 None:intergenic 35.0%
GATATAGAAACTGCAATTGC+AGG - chr2_2:53058759-53058778 None:intergenic 35.0%
TAATATATGCGGCTACATTC+AGG + chr2_2:53059238-53059257 Msa0257320:intron 35.0%
CAAGAAAAGAAAGGTAGGTA+TGG - chr2_2:53059291-53059310 None:intergenic 35.0%
CCATTCAAGAAAAGAAAGGT+AGG - chr2_2:53059296-53059315 None:intergenic 35.0%
! CCTACCTTTCTTTTCTTGAA+TGG + chr2_2:53059293-53059312 Msa0257320:CDS 35.0%
! ACCTTTCTTTTCTTGAATGG+TGG + chr2_2:53059296-53059315 Msa0257320:CDS 35.0%
TCCACCATTCAAGAAAAGAA+AGG - chr2_2:53059300-53059319 None:intergenic 35.0%
AACTCTTGTGGACAAAATTG+TGG + chr2_2:53059319-53059338 Msa0257320:CDS 35.0%
ACTCTTGTGGACAAAATTGT+GGG + chr2_2:53059320-53059339 Msa0257320:CDS 35.0%
GTGCAAATCCTGATGAAATA+AGG + chr2_2:53059342-53059361 Msa0257320:CDS 35.0%
!! ACCCTTTTCCTTATTTCATC+AGG - chr2_2:53059353-53059372 None:intergenic 35.0%
!!! AACTATAGTTTTGGACCTAC+TGG + chr2_2:53059466-53059485 Msa0257320:three_prime_UTR 35.0%
ACCAAAATGATCTCTTAAGC+CGG - chr2_2:53058225-53058244 None:intergenic 35.0%
GATGCCAATTCTTGAAAGAA+AGG - chr2_2:53058314-53058333 None:intergenic 35.0%
AGATGTTCTCTTTCTCACAT+TGG + chr2_2:53058343-53058362 Msa0257320:CDS 35.0%
CCGAATACAAACACTTTAGA+TGG - chr2_2:53058526-53058545 None:intergenic 35.0%
! AAGGAGCATATGATACATAG+TGG - chr2_2:53058559-53058578 None:intergenic 35.0%
GATATAGAAACTGCAATTGC+AGG - chr2_2:53058759-53058778 None:intergenic 35.0%
TAATATATGCGGCTACATTC+AGG + chr2_2:53059238-53059257 Msa0257320:intron 35.0%
CAAGAAAAGAAAGGTAGGTA+TGG - chr2_2:53059291-53059310 None:intergenic 35.0%
CCATTCAAGAAAAGAAAGGT+AGG - chr2_2:53059296-53059315 None:intergenic 35.0%
! CCTACCTTTCTTTTCTTGAA+TGG + chr2_2:53059293-53059312 Msa0257320:CDS 35.0%
! ACCTTTCTTTTCTTGAATGG+TGG + chr2_2:53059296-53059315 Msa0257320:CDS 35.0%
TCCACCATTCAAGAAAAGAA+AGG - chr2_2:53059300-53059319 None:intergenic 35.0%
AACTCTTGTGGACAAAATTG+TGG + chr2_2:53059319-53059338 Msa0257320:CDS 35.0%
ACTCTTGTGGACAAAATTGT+GGG + chr2_2:53059320-53059339 Msa0257320:CDS 35.0%
GTGCAAATCCTGATGAAATA+AGG + chr2_2:53059342-53059361 Msa0257320:CDS 35.0%
!! ACCCTTTTCCTTATTTCATC+AGG - chr2_2:53059353-53059372 None:intergenic 35.0%
!!! AACTATAGTTTTGGACCTAC+TGG + chr2_2:53059466-53059485 Msa0257320:three_prime_UTR 35.0%
ACTTACGGGGTAACTTTGAT+TGG - chr2_2:53057980-53057999 None:intergenic 40.0%
TGTCAAAATCACGTTACCAG+TGG + chr2_2:53058110-53058129 Msa0257320:intron 40.0%
!! ACCGGCTTAAGAGATCATTT+TGG + chr2_2:53058221-53058240 Msa0257320:intron 40.0%
TCTCTTTCTCACATTGGATG+TGG + chr2_2:53058349-53058368 Msa0257320:CDS 40.0%
ATTGGATGTGGATGAGGTTA+AGG + chr2_2:53058361-53058380 Msa0257320:CDS 40.0%
CATTTGCATGTCTTGGCAAA+TGG - chr2_2:53058464-53058483 None:intergenic 40.0%
CATTTGCCAAGACATGCAAA+TGG + chr2_2:53058462-53058481 Msa0257320:intron 40.0%
! GGCTTTAATTTCCATCTTGG+AGG - chr2_2:53059270-53059289 None:intergenic 40.0%
AAACACACACAACAACCAGT+AGG - chr2_2:53059484-53059503 None:intergenic 40.0%
! TACTGGTTGTTGTGTGTGTT+TGG + chr2_2:53059483-53059502 Msa0257320:three_prime_UTR 40.0%
ACTGGTTGTTGTGTGTGTTT+GGG + chr2_2:53059484-53059503 Msa0257320:three_prime_UTR 40.0%
ACTTACGGGGTAACTTTGAT+TGG - chr2_2:53057980-53057999 None:intergenic 40.0%
TGTCAAAATCACGTTACCAG+TGG + chr2_2:53058110-53058129 Msa0257320:intron 40.0%
!! ACCGGCTTAAGAGATCATTT+TGG + chr2_2:53058221-53058240 Msa0257320:intron 40.0%
TCTCTTTCTCACATTGGATG+TGG + chr2_2:53058349-53058368 Msa0257320:CDS 40.0%
ATTGGATGTGGATGAGGTTA+AGG + chr2_2:53058361-53058380 Msa0257320:CDS 40.0%
CATTTGCATGTCTTGGCAAA+TGG - chr2_2:53058464-53058483 None:intergenic 40.0%
CATTTGCCAAGACATGCAAA+TGG + chr2_2:53058462-53058481 Msa0257320:intron 40.0%
! GGCTTTAATTTCCATCTTGG+AGG - chr2_2:53059270-53059289 None:intergenic 40.0%
AAACACACACAACAACCAGT+AGG - chr2_2:53059484-53059503 None:intergenic 40.0%
! TACTGGTTGTTGTGTGTGTT+TGG + chr2_2:53059483-53059502 Msa0257320:three_prime_UTR 40.0%
ACTGGTTGTTGTGTGTGTTT+GGG + chr2_2:53059484-53059503 Msa0257320:three_prime_UTR 40.0%
TCTCACATTGGATGTGGATG+AGG + chr2_2:53058355-53058374 Msa0257320:CDS 45.0%
TCAGGAAATTGCCTCCAAGA+TGG + chr2_2:53059256-53059275 Msa0257320:intron 45.0%
! CTTGAATGGTGGAACTCTTG+TGG + chr2_2:53059307-53059326 Msa0257320:CDS 45.0%
TCTCACATTGGATGTGGATG+AGG + chr2_2:53058355-53058374 Msa0257320:CDS 45.0%
TCAGGAAATTGCCTCCAAGA+TGG + chr2_2:53059256-53059275 Msa0257320:intron 45.0%
! CTTGAATGGTGGAACTCTTG+TGG + chr2_2:53059307-53059326 Msa0257320:CDS 45.0%
!! ATAATAAAATAATTTAAAAG+AGG - chr2_2:53058914-53058933 None:intergenic 5.0%
!!! TATAATAAAATATTTTTGTT+TGG - chr2_2:53058943-53058962 None:intergenic 5.0%
!!! TATAATAAATTAATCATTTT+TGG + chr2_2:53058959-53058978 Msa0257320:intron 5.0%
!! TTATAATTAATTTATGATAT+AGG - chr2_2:53059141-53059160 None:intergenic 5.0%
!! ATAATAAAATAATTTAAAAG+AGG - chr2_2:53058914-53058933 None:intergenic 5.0%
!!! TATAATAAAATATTTTTGTT+TGG - chr2_2:53058943-53058962 None:intergenic 5.0%
!!! TATAATAAATTAATCATTTT+TGG + chr2_2:53058959-53058978 Msa0257320:intron 5.0%
!! TTATAATTAATTTATGATAT+AGG - chr2_2:53059141-53059160 None:intergenic 5.0%
! GCATAATCACAGAGCACCAC+TGG - chr2_2:53058129-53058148 None:intergenic 50.0%
! GCATAATCACAGAGCACCAC+TGG - chr2_2:53058129-53058148 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 53057870 53059829 53057870 ID=Msa0257320;Name=Msa0257320
chr2_2 mRNA 53057870 53059829 53057870 ID=Msa0257320-mRNA-1;Parent=Msa0257320;Name=Msa0257320-mRNA-1;_AED=0.05;_eAED=0.05;_QI=22|1|1|1|0.5|0.66|3|426|122
chr2_2 exon 53057870 53057993 53057870 ID=Msa0257320-mRNA-1:exon:15882;Parent=Msa0257320-mRNA-1
chr2_2 exon 53058260 53058382 53058260 ID=Msa0257320-mRNA-1:exon:15883;Parent=Msa0257320-mRNA-1
chr2_2 exon 53059260 53059829 53059260 ID=Msa0257320-mRNA-1:exon:15884;Parent=Msa0257320-mRNA-1
chr2_2 five_prime_UTR 53057870 53057891 53057870 ID=Msa0257320-mRNA-1:five_prime_utr;Parent=Msa0257320-mRNA-1
chr2_2 CDS 53057892 53057993 53057892 ID=Msa0257320-mRNA-1:cds;Parent=Msa0257320-mRNA-1
chr2_2 CDS 53058260 53058382 53058260 ID=Msa0257320-mRNA-1:cds;Parent=Msa0257320-mRNA-1
chr2_2 CDS 53059260 53059403 53059260 ID=Msa0257320-mRNA-1:cds;Parent=Msa0257320-mRNA-1
chr2_2 three_prime_UTR 53059404 53059829 53059404 ID=Msa0257320-mRNA-1:three_prime_utr;Parent=Msa0257320-mRNA-1
Gene Sequence

>Msa0257320

ATGGAGGGCAAAAAATATCTCAAAAAGTCTAAGGTTATCAAAATAGACTCAAAAAAATCATGGGAACATTACATCTCTTATGCTACCAATCAAAGTTACCCCGTTGTGGTTCATTTTTCTGCTTTTTGGTGTGTTCCCTCTATACAAATGAATCCTTTCTTTCAAGAATTGGCATCAAACTATCAAGATGTTCTCTTTCTCACATTGGATGTGGATGAGGTTAAGGAAATTGCCTCCAAGATGGAAATTAAAGCCATACCTACCTTTCTTTTCTTGAATGGTGGAACTCTTGTGGACAAAATTGTGGGTGCAAATCCTGATGAAATAAGGAAAAGGGTTGATCATTTTATTCAATCAACTCCTTCCTAA
Protein sequence

>Msa0257320

MEGKKYLKKSKVIKIDSKKSWEHYISYATNQSYPVVVHFSAFWCVPSIQMNPFFQELASNYQDVLFLTLDVDEVKEIASKMEIKAIPTFLFLNGGTLVDKIVGANPDEIRKRVDHFIQSTPS*