AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1147640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1147640 MtrunA17_Chr7g0234601 96.319 163 6 0 1 163 1 163 2.38e-100 290
Msa1147640 MtrunA17_Chr7g0249501 55.556 135 58 2 28 161 33 166 4.90e-49 159
Msa1147640 MtrunA17_Chr2g0280591 50.435 115 57 0 34 148 26 140 1.31e-36 127
Msa1147640 MtrunA17_Chr4g0048881 44.361 133 73 1 16 148 12 143 1.45e-34 122
Msa1147640 MtrunA17_Chr4g0042211 44.248 113 63 0 36 148 29 141 1.12e-32 117
Msa1147640 MtrunA17_Chr4g0048861 40.678 118 70 0 31 148 26 143 3.63e-30 110
Msa1147640 MtrunA17_Chr4g0031861 42.373 118 68 0 31 148 26 143 3.61e-29 107
Msa1147640 MtrunA17_Chr8g0378491 39.474 114 69 0 35 148 29 142 1.05e-27 104
Msa1147640 MtrunA17_Chr4g0048871 39.552 134 79 2 16 148 12 144 1.75e-27 103
Msa1147640 MtrunA17_Chr8g0378501 31.343 134 90 2 27 159 22 154 1.53e-23 93.2
Msa1147640 MtrunA17_Chr7g0269431 30.657 137 89 4 12 143 4 139 2.78e-13 65.5
Msa1147640 MtrunA17_Chr4g0061181 30.000 120 80 3 35 151 53 171 3.69e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1147640 AT3G07470.3 57.639 144 59 2 11 152 5 148 1.25e-54 173
Msa1147640 AT3G07470.2 57.639 144 59 2 11 152 5 148 1.95e-54 172
Msa1147640 AT3G07470.1 57.639 144 59 2 11 152 5 148 2.43e-54 172
Msa1147640 AT3G07460.1 53.595 153 68 2 5 156 1 151 1.35e-50 163
Msa1147640 AT3G07460.2 54.305 151 66 2 5 154 1 149 1.36e-48 161
Msa1147640 AT5G16380.1 48.936 141 69 2 16 153 9 149 2.24e-37 130
Msa1147640 AT1G61667.1 47.107 121 63 1 29 148 15 135 1.08e-33 119
Msa1147640 AT1G61667.2 47.107 121 63 1 29 148 51 171 2.02e-33 120
Msa1147640 AT5G54530.1 45.614 114 62 0 35 148 28 141 8.09e-32 115
Msa1147640 AT5G19860.1 39.130 115 70 0 34 148 34 148 4.14e-27 103
Msa1147640 AT1G55265.1 36.752 117 72 2 33 148 51 166 2.11e-23 93.6
Msa1147640 AT1G02813.1 31.387 137 87 4 14 148 5 136 1.88e-14 68.9
Msa1147640 AT4G02360.1 30.435 138 88 4 13 148 9 140 4.22e-13 65.5

Find 57 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTATGATCGAAACGTTTC+TGG 0.129281 7_4:-27021530 Msa1147640:CDS
AAGAGTGAAGAGATGGGTTT+TGG 0.181930 7_4:+27021920 None:intergenic
TTCCAATGCGAAACAGATTC+TGG 0.243077 7_4:+27020070 None:intergenic
ATTCAGGAGGAGTTTCAAAA+AGG 0.298945 7_4:+27021354 None:intergenic
CAATGTTGTATAGGAAGAAT+TGG 0.307923 7_4:-27021113 Msa1147640:intron
TTGAACTCGTTTACACCCTT+TGG 0.312429 7_4:+27021623 None:intergenic
TCGACACGAATACTCATAAC+AGG 0.354265 7_4:+27021440 None:intergenic
TTGACGAAGATGGTAAATTC+TGG 0.360678 7_4:-27021598 Msa1147640:CDS
CGTGTCGATGTTCCTAGCTC+TGG 0.386889 7_4:-27021425 Msa1147640:CDS
TGAACTCGTTTACACCCTTT+GGG 0.387144 7_4:+27021624 None:intergenic
TGGGATTGGTGAAGAGAAGA+AGG 0.393080 7_4:+27021940 None:intergenic
GAGATTGAAGAGTGAAGAGA+TGG 0.394228 7_4:+27021913 None:intergenic
GGGTTGAAAATGAAAGAATA+AGG 0.394314 7_4:+27021723 None:intergenic
TATAGGAAGAATTGGGAAAA+GGG 0.401597 7_4:-27021105 Msa1147640:intron
TGGAACTTGTTGAAGAAGAA+AGG 0.404734 7_4:+27021702 None:intergenic
GTTTCTGGGTCATTGAGTTA+TGG 0.408980 7_4:-27021515 Msa1147640:CDS
GTACGAACAGCGACACATTC+AGG 0.416424 7_4:+27021338 None:intergenic
TCTGTTTCGCATTGGAAAGA+TGG 0.424770 7_4:-27020064 Msa1147640:CDS
GGAAAGATCGATGCTTTAAC+CGG 0.427520 7_4:-27021494 Msa1147640:CDS
AATGTTGTATAGGAAGAATT+GGG 0.440724 7_4:-27021112 Msa1147640:intron
GGTTTGATTTACTTTGATGT+TGG 0.443990 7_4:-27021404 Msa1147640:CDS
ATTGAGGAATAGAGAGAGAG+AGG 0.453012 7_4:+27021872 None:intergenic
GCTTGAATACAGAAGGGATT+AGG 0.453697 7_4:+27021979 None:intergenic
GTATAGGAAGAATTGGGAAA+AGG 0.455349 7_4:-27021106 Msa1147640:intron
TTCCAGAATCTGTTTCGCAT+TGG 0.464353 7_4:-27020072 Msa1147640:CDS
TAGAGGCAGCCACTGTGTAT+TGG 0.469459 7_4:-27020117 Msa1147640:CDS
GGAACTTGTTGAAGAAGAAA+GGG 0.469817 7_4:+27021703 None:intergenic
TTGAGGAATAGAGAGAGAGA+GGG 0.471490 7_4:+27021873 None:intergenic
AAGATCTTGAGCTTCTAAAC+CGG 0.488608 7_4:+27021475 None:intergenic
TGAAGAGAAGAAGGAGAGAG+AGG 0.491273 7_4:+27021949 None:intergenic
TTCAACCATGGTCTTCCAAT+GGG 0.492229 7_4:-27021653 Msa1147640:CDS
CTCGTAGATTGATGTTGCAT+TGG 0.499306 7_4:+27021682 None:intergenic
TGACGAAGATGGTAAATTCT+GGG 0.507232 7_4:-27021597 Msa1147640:CDS
ATTATGATCGAAACGTTTCT+GGG 0.508412 7_4:-27021529 Msa1147640:CDS
ATTCAACCATGGTCTTCCAA+TGG 0.521264 7_4:-27021654 Msa1147640:CDS
AGATTGAAGAGTGAAGAGAT+GGG 0.523392 7_4:+27021914 None:intergenic
AATGGTTGTTATGTAAGAGA+AGG 0.531584 7_4:+27021754 None:intergenic
GAGTTCAACGTTGACGAAGA+TGG 0.533830 7_4:-27021608 Msa1147640:CDS
TACTCATAACAGGGAACCAA+AGG 0.534228 7_4:+27021450 None:intergenic
AAGAATAAGGAAGAATTGAA+TGG 0.537672 7_4:+27021736 None:intergenic
AAGTAAATCAAACCAGAGCT+AGG 0.539278 7_4:+27021413 None:intergenic
CCCTTTGGGAAAATTCCCAT+TGG 0.542624 7_4:+27021638 None:intergenic
GAAGAGAAGAAGGAGAGAGA+GGG 0.542821 7_4:+27021950 None:intergenic
TGCAGAATTTAGTGTAGTAG+AGG 0.545786 7_4:-27020134 Msa1147640:CDS
CAGCCACTGTGTATTGGAAA+GGG 0.548916 7_4:-27020111 Msa1147640:CDS
GTTGATTGAGATATTTGTCA+TGG 0.549109 7_4:-27021083 Msa1147640:CDS
GCAGCCACTGTGTATTGGAA+AGG 0.560585 7_4:-27020112 Msa1147640:CDS
GTTGACAGCTTGAATACAGA+AGG 0.567894 7_4:+27021972 None:intergenic
GTTGTGATGGTTGTAGATTG+AGG 0.572632 7_4:+27021856 None:intergenic
TCGTACAGACGATGATTCCA+AGG 0.615763 7_4:-27021321 Msa1147640:intron
AAATTCCCATTGGAAGACCA+TGG 0.630868 7_4:+27021648 None:intergenic
TTGACAGCTTGAATACAGAA+GGG 0.647884 7_4:+27021973 None:intergenic
CTTCCCTTTCCAATACACAG+TGG 0.651593 7_4:+27020108 None:intergenic
TACGAGGTTCTATTCAACCA+TGG 0.660331 7_4:-27021665 Msa1147640:CDS
CGACACGAATACTCATAACA+GGG 0.676336 7_4:+27021441 None:intergenic
CAATGCAACATCAATCTACG+AGG 0.716978 7_4:-27021681 Msa1147640:CDS
CGAACAGCGACACATTCAGG+AGG 0.736323 7_4:+27021341 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTTTTTGTTTTTGTTTT+TGG + chr7_4:27020176-27020195 None:intergenic 15.0%
!!! TTTTTATTGGATAACTTTAA+CGG - chr7_4:27021138-27021157 Msa1147640:intron 15.0%
!!! TGTTTTTTTGTTTTTGTTTT+TGG + chr7_4:27020176-27020195 None:intergenic 15.0%
!!! TTTTTATTGGATAACTTTAA+CGG - chr7_4:27021138-27021157 Msa1147640:intron 15.0%
!!! TTTGTTTTTGTTTTTGGTTT+TGG + chr7_4:27020170-27020189 None:intergenic 20.0%
!! ACTTAAAAAATGTCAGAATT+GGG + chr7_4:27020730-27020749 None:intergenic 20.0%
!! AACTTAAAAAATGTCAGAAT+TGG + chr7_4:27020731-27020750 None:intergenic 20.0%
!!! TTTTGTTCTGTTATAATTCA+TGG - chr7_4:27021396-27021415 Msa1147640:CDS 20.0%
!!! TTTGTTTTTGTTTTTGGTTT+TGG + chr7_4:27020170-27020189 None:intergenic 20.0%
!! ACTTAAAAAATGTCAGAATT+GGG + chr7_4:27020730-27020749 None:intergenic 20.0%
!! AACTTAAAAAATGTCAGAAT+TGG + chr7_4:27020731-27020750 None:intergenic 20.0%
!!! TTTTGTTCTGTTATAATTCA+TGG - chr7_4:27021396-27021415 Msa1147640:CDS 20.0%
!!! TTTTAAAGTTTTGGTTGTGA+TGG + chr7_4:27020128-27020147 None:intergenic 25.0%
!!! GTTTGAGAGTTTTAAAGTTT+TGG + chr7_4:27020137-27020156 None:intergenic 25.0%
! AAGAATAAGGAAGAATTGAA+TGG + chr7_4:27020235-27020254 None:intergenic 25.0%
!!! TTTTAAGTTAGTACCTGAAT+TGG - chr7_4:27020742-27020761 Msa1147640:intron 25.0%
!! TTTAAGTTAGTACCTGAATT+GGG - chr7_4:27020743-27020762 Msa1147640:intron 25.0%
!! TCTTGTGTTTGATTTATGAT+TGG - chr7_4:27020807-27020826 Msa1147640:intron 25.0%
! AATGTTGTATAGGAAGAATT+GGG - chr7_4:27020856-27020875 Msa1147640:intron 25.0%
!! CACTTTTATCATATGTTGTT+TGG - chr7_4:27020974-27020993 Msa1147640:intron 25.0%
!!! TTTTATCATATGTTGTTTGG+TGG - chr7_4:27020977-27020996 Msa1147640:intron 25.0%
!! TTTATCATATGTTGTTTGGT+GGG - chr7_4:27020978-27020997 Msa1147640:intron 25.0%
!! TTATAAGCTTTTTGATGCAT+AGG - chr7_4:27021089-27021108 Msa1147640:CDS 25.0%
!!! AAACTTTTTTGTTTCTGCTT+TGG - chr7_4:27021113-27021132 Msa1147640:intron 25.0%
!!! TTCTGCTTTGGTTTTTTTAT+TGG - chr7_4:27021125-27021144 Msa1147640:intron 25.0%
!! TTAACGGTTTTTCTTTAGTT+TGG - chr7_4:27021154-27021173 Msa1147640:intron 25.0%
!!! TTTTTCTTTAGTTTGGTGTA+TGG - chr7_4:27021161-27021180 Msa1147640:intron 25.0%
! TATTTATTGTCATGCTTTGA+TGG - chr7_4:27021308-27021327 Msa1147640:intron 25.0%
! TCATTGTATCAAAAGTCAAA+AGG + chr7_4:27021338-27021357 None:intergenic 25.0%
!! CTTTTGATACAATGATTGAA+TGG - chr7_4:27021343-27021362 Msa1147640:CDS 25.0%
! GAATTATAACAGAACAAAAC+AGG + chr7_4:27021395-27021414 None:intergenic 25.0%
!!! TGCTTTTTCATGTATCTTAA+TGG - chr7_4:27021486-27021505 Msa1147640:CDS 25.0%
! ATAAGATGTTTGTGATTTGA+AGG + chr7_4:27021554-27021573 None:intergenic 25.0%
!! ATTTTTAAATAAGAACAGCG+AGG + chr7_4:27021578-27021597 None:intergenic 25.0%
!! TTTCTTGATCTAGTTTATAG+CGG + chr7_4:27021649-27021668 None:intergenic 25.0%
!! TTCGAGGAGTATAAATTAAT+AGG - chr7_4:27021762-27021781 Msa1147640:CDS 25.0%
! AATTAATAGGTCCAATGATT+CGG - chr7_4:27021775-27021794 Msa1147640:CDS 25.0%
!!! AATAGAGCATTTTGTTGAAA+TGG - chr7_4:27021933-27021952 Msa1147640:five_prime_UTR 25.0%
!!! TTTTAAAGTTTTGGTTGTGA+TGG + chr7_4:27020128-27020147 None:intergenic 25.0%
!!! GTTTGAGAGTTTTAAAGTTT+TGG + chr7_4:27020137-27020156 None:intergenic 25.0%
! AAGAATAAGGAAGAATTGAA+TGG + chr7_4:27020235-27020254 None:intergenic 25.0%
!!! TTTTAAGTTAGTACCTGAAT+TGG - chr7_4:27020742-27020761 Msa1147640:intron 25.0%
!! TTTAAGTTAGTACCTGAATT+GGG - chr7_4:27020743-27020762 Msa1147640:intron 25.0%
!! TCTTGTGTTTGATTTATGAT+TGG - chr7_4:27020807-27020826 Msa1147640:intron 25.0%
! AATGTTGTATAGGAAGAATT+GGG - chr7_4:27020856-27020875 Msa1147640:intron 25.0%
!! CACTTTTATCATATGTTGTT+TGG - chr7_4:27020974-27020993 Msa1147640:intron 25.0%
!!! TTTTATCATATGTTGTTTGG+TGG - chr7_4:27020977-27020996 Msa1147640:intron 25.0%
!! TTTATCATATGTTGTTTGGT+GGG - chr7_4:27020978-27020997 Msa1147640:intron 25.0%
!! TTATAAGCTTTTTGATGCAT+AGG - chr7_4:27021089-27021108 Msa1147640:CDS 25.0%
!!! AAACTTTTTTGTTTCTGCTT+TGG - chr7_4:27021113-27021132 Msa1147640:intron 25.0%
!!! TTCTGCTTTGGTTTTTTTAT+TGG - chr7_4:27021125-27021144 Msa1147640:intron 25.0%
!! TTAACGGTTTTTCTTTAGTT+TGG - chr7_4:27021154-27021173 Msa1147640:intron 25.0%
!!! TTTTTCTTTAGTTTGGTGTA+TGG - chr7_4:27021161-27021180 Msa1147640:intron 25.0%
! TATTTATTGTCATGCTTTGA+TGG - chr7_4:27021308-27021327 Msa1147640:intron 25.0%
! TCATTGTATCAAAAGTCAAA+AGG + chr7_4:27021338-27021357 None:intergenic 25.0%
!! CTTTTGATACAATGATTGAA+TGG - chr7_4:27021343-27021362 Msa1147640:CDS 25.0%
! GAATTATAACAGAACAAAAC+AGG + chr7_4:27021395-27021414 None:intergenic 25.0%
!!! TGCTTTTTCATGTATCTTAA+TGG - chr7_4:27021486-27021505 Msa1147640:CDS 25.0%
! ATAAGATGTTTGTGATTTGA+AGG + chr7_4:27021554-27021573 None:intergenic 25.0%
!! ATTTTTAAATAAGAACAGCG+AGG + chr7_4:27021578-27021597 None:intergenic 25.0%
!! TTTCTTGATCTAGTTTATAG+CGG + chr7_4:27021649-27021668 None:intergenic 25.0%
!! TTCGAGGAGTATAAATTAAT+AGG - chr7_4:27021762-27021781 Msa1147640:CDS 25.0%
! AATTAATAGGTCCAATGATT+CGG - chr7_4:27021775-27021794 Msa1147640:CDS 25.0%
!!! AATAGAGCATTTTGTTGAAA+TGG - chr7_4:27021933-27021952 Msa1147640:five_prime_UTR 25.0%
AATGGTTGTTATGTAAGAGA+AGG + chr7_4:27020217-27020236 None:intergenic 30.0%
GGGTTGAAAATGAAAGAATA+AGG + chr7_4:27020248-27020267 None:intergenic 30.0%
ATTATGATCGAAACGTTTCT+GGG - chr7_4:27020439-27020458 Msa1147640:intron 30.0%
! GGTTTGATTTACTTTGATGT+TGG - chr7_4:27020564-27020583 Msa1147640:intron 30.0%
CTTTATTCGAGAGAAAAAAG+AGG + chr7_4:27020675-27020694 None:intergenic 30.0%
! TCTCGAATAAAGATCCATTT+TGG - chr7_4:27020683-27020702 Msa1147640:intron 30.0%
TTGTGTTGTCAATGTTGTAT+AGG - chr7_4:27020846-27020865 Msa1147640:intron 30.0%
CAATGTTGTATAGGAAGAAT+TGG - chr7_4:27020855-27020874 Msa1147640:intron 30.0%
TATAGGAAGAATTGGGAAAA+GGG - chr7_4:27020863-27020882 Msa1147640:intron 30.0%
GTTGATTGAGATATTTGTCA+TGG - chr7_4:27020885-27020904 Msa1147640:intron 30.0%
! GTTGTGATTGTGATTTTGTT+TGG - chr7_4:27020928-27020947 Msa1147640:intron 30.0%
GATTGAATGGAATGAATTCT+TGG - chr7_4:27021356-27021375 Msa1147640:CDS 30.0%
TATAATTCATGGTTGTGATG+AGG - chr7_4:27021407-27021426 Msa1147640:CDS 30.0%
ATCATACAAGAAACTGTTGA+AGG + chr7_4:27021435-27021454 None:intergenic 30.0%
TTCAACAGTTTCTTGTATGA+TGG - chr7_4:27021434-27021453 Msa1147640:CDS 30.0%
AAGATACATGAAAAAGCAAG+AGG + chr7_4:27021484-27021503 None:intergenic 30.0%
AAACTAGATCAAGAAACTTC+TGG - chr7_4:27021653-27021672 Msa1147640:CDS 30.0%
TCGAATGAATACAATCTGAT+TGG + chr7_4:27021747-27021766 None:intergenic 30.0%
AATCAGATTGTATTCATTCG+AGG - chr7_4:27021746-27021765 Msa1147640:CDS 30.0%
ATAACACAAACGACAACAAT+AGG + chr7_4:27021802-27021821 None:intergenic 30.0%
!! CATTTTGTTGAAATGGATTC+AGG - chr7_4:27021940-27021959 Msa1147640:five_prime_UTR 30.0%
AATGGTTGTTATGTAAGAGA+AGG + chr7_4:27020217-27020236 None:intergenic 30.0%
GGGTTGAAAATGAAAGAATA+AGG + chr7_4:27020248-27020267 None:intergenic 30.0%
ATTATGATCGAAACGTTTCT+GGG - chr7_4:27020439-27020458 Msa1147640:intron 30.0%
! GGTTTGATTTACTTTGATGT+TGG - chr7_4:27020564-27020583 Msa1147640:intron 30.0%
CTTTATTCGAGAGAAAAAAG+AGG + chr7_4:27020675-27020694 None:intergenic 30.0%
! TCTCGAATAAAGATCCATTT+TGG - chr7_4:27020683-27020702 Msa1147640:intron 30.0%
TTGTGTTGTCAATGTTGTAT+AGG - chr7_4:27020846-27020865 Msa1147640:intron 30.0%
CAATGTTGTATAGGAAGAAT+TGG - chr7_4:27020855-27020874 Msa1147640:intron 30.0%
TATAGGAAGAATTGGGAAAA+GGG - chr7_4:27020863-27020882 Msa1147640:intron 30.0%
GTTGATTGAGATATTTGTCA+TGG - chr7_4:27020885-27020904 Msa1147640:intron 30.0%
! GTTGTGATTGTGATTTTGTT+TGG - chr7_4:27020928-27020947 Msa1147640:intron 30.0%
GATTGAATGGAATGAATTCT+TGG - chr7_4:27021356-27021375 Msa1147640:CDS 30.0%
TATAATTCATGGTTGTGATG+AGG - chr7_4:27021407-27021426 Msa1147640:CDS 30.0%
ATCATACAAGAAACTGTTGA+AGG + chr7_4:27021435-27021454 None:intergenic 30.0%
TTCAACAGTTTCTTGTATGA+TGG - chr7_4:27021434-27021453 Msa1147640:CDS 30.0%
AAGATACATGAAAAAGCAAG+AGG + chr7_4:27021484-27021503 None:intergenic 30.0%
AAACTAGATCAAGAAACTTC+TGG - chr7_4:27021653-27021672 Msa1147640:CDS 30.0%
TCGAATGAATACAATCTGAT+TGG + chr7_4:27021747-27021766 None:intergenic 30.0%
AATCAGATTGTATTCATTCG+AGG - chr7_4:27021746-27021765 Msa1147640:CDS 30.0%
ATAACACAAACGACAACAAT+AGG + chr7_4:27021802-27021821 None:intergenic 30.0%
!! CATTTTGTTGAAATGGATTC+AGG - chr7_4:27021940-27021959 Msa1147640:five_prime_UTR 30.0%
TTGACAGCTTGAATACAGAA+GGG + chr7_4:27019998-27020017 None:intergenic 35.0%
AGATTGAAGAGTGAAGAGAT+GGG + chr7_4:27020057-27020076 None:intergenic 35.0%
!! GGAACTTGTTGAAGAAGAAA+GGG + chr7_4:27020268-27020287 None:intergenic 35.0%
!! TGGAACTTGTTGAAGAAGAA+AGG + chr7_4:27020269-27020288 None:intergenic 35.0%
TTGACGAAGATGGTAAATTC+TGG - chr7_4:27020370-27020389 Msa1147640:intron 35.0%
TGACGAAGATGGTAAATTCT+GGG - chr7_4:27020371-27020390 Msa1147640:intron 35.0%
CATTATGATCGAAACGTTTC+TGG - chr7_4:27020438-27020457 Msa1147640:intron 35.0%
AAGATCTTGAGCTTCTAAAC+CGG + chr7_4:27020496-27020515 None:intergenic 35.0%
! AAGCTCAAGATCTTTTCCTT+TGG - chr7_4:27020502-27020521 Msa1147640:intron 35.0%
AAGTAAATCAAACCAGAGCT+AGG + chr7_4:27020558-27020577 None:intergenic 35.0%
TTCAGGAGGAGTTTCAAAAA+GGG + chr7_4:27020616-27020635 None:intergenic 35.0%
ATTCAGGAGGAGTTTCAAAA+AGG + chr7_4:27020617-27020636 None:intergenic 35.0%
GAAATTGTGAAGGACCAAAA+TGG + chr7_4:27020700-27020719 None:intergenic 35.0%
AGACCAGAAACAAAACAACT+AGG + chr7_4:27020790-27020809 None:intergenic 35.0%
GTATAGGAAGAATTGGGAAA+AGG - chr7_4:27020862-27020881 Msa1147640:intron 35.0%
!! GTGATTGTGATTTTGTTTGG+TGG - chr7_4:27020931-27020950 Msa1147640:intron 35.0%
!! ATTGTGATTTTGTTTGGTGG+AGG - chr7_4:27020934-27020953 Msa1147640:intron 35.0%
!! TTGTGATTTTGTTTGGTGGA+GGG - chr7_4:27020935-27020954 Msa1147640:intron 35.0%
TCTTGTGTTGTCAATTGTCA+TGG - chr7_4:27021056-27021075 Msa1147640:CDS 35.0%
TGACTAATAACCTATGCACA+TGG - chr7_4:27021226-27021245 Msa1147640:intron 35.0%
!! CAAGTATTTTGTTGTACGCA+GGG - chr7_4:27021608-27021627 Msa1147640:CDS 35.0%
GATGTTCTATAGATCAAAGC+AGG - chr7_4:27021680-27021699 Msa1147640:CDS 35.0%
TCTATAGATCAAAGCAGGAA+AGG - chr7_4:27021685-27021704 Msa1147640:CDS 35.0%
CTATAGATCAAAGCAGGAAA+GGG - chr7_4:27021686-27021705 Msa1147640:CDS 35.0%
TTTGTGTAGCAAAATCTCCA+TGG - chr7_4:27021716-27021735 Msa1147640:CDS 35.0%
TGCAGAATTTAGTGTAGTAG+AGG - chr7_4:27021834-27021853 Msa1147640:five_prime_UTR 35.0%
TTGACAGCTTGAATACAGAA+GGG + chr7_4:27019998-27020017 None:intergenic 35.0%
AGATTGAAGAGTGAAGAGAT+GGG + chr7_4:27020057-27020076 None:intergenic 35.0%
!! GGAACTTGTTGAAGAAGAAA+GGG + chr7_4:27020268-27020287 None:intergenic 35.0%
!! TGGAACTTGTTGAAGAAGAA+AGG + chr7_4:27020269-27020288 None:intergenic 35.0%
TTGACGAAGATGGTAAATTC+TGG - chr7_4:27020370-27020389 Msa1147640:intron 35.0%
TGACGAAGATGGTAAATTCT+GGG - chr7_4:27020371-27020390 Msa1147640:intron 35.0%
CATTATGATCGAAACGTTTC+TGG - chr7_4:27020438-27020457 Msa1147640:intron 35.0%
AAGATCTTGAGCTTCTAAAC+CGG + chr7_4:27020496-27020515 None:intergenic 35.0%
! AAGCTCAAGATCTTTTCCTT+TGG - chr7_4:27020502-27020521 Msa1147640:intron 35.0%
AAGTAAATCAAACCAGAGCT+AGG + chr7_4:27020558-27020577 None:intergenic 35.0%
TTCAGGAGGAGTTTCAAAAA+GGG + chr7_4:27020616-27020635 None:intergenic 35.0%
ATTCAGGAGGAGTTTCAAAA+AGG + chr7_4:27020617-27020636 None:intergenic 35.0%
GAAATTGTGAAGGACCAAAA+TGG + chr7_4:27020700-27020719 None:intergenic 35.0%
AGACCAGAAACAAAACAACT+AGG + chr7_4:27020790-27020809 None:intergenic 35.0%
GTATAGGAAGAATTGGGAAA+AGG - chr7_4:27020862-27020881 Msa1147640:intron 35.0%
!! GTGATTGTGATTTTGTTTGG+TGG - chr7_4:27020931-27020950 Msa1147640:intron 35.0%
!! ATTGTGATTTTGTTTGGTGG+AGG - chr7_4:27020934-27020953 Msa1147640:intron 35.0%
!! TTGTGATTTTGTTTGGTGGA+GGG - chr7_4:27020935-27020954 Msa1147640:intron 35.0%
TCTTGTGTTGTCAATTGTCA+TGG - chr7_4:27021056-27021075 Msa1147640:CDS 35.0%
TGACTAATAACCTATGCACA+TGG - chr7_4:27021226-27021245 Msa1147640:intron 35.0%
!! CAAGTATTTTGTTGTACGCA+GGG - chr7_4:27021608-27021627 Msa1147640:CDS 35.0%
GATGTTCTATAGATCAAAGC+AGG - chr7_4:27021680-27021699 Msa1147640:CDS 35.0%
TCTATAGATCAAAGCAGGAA+AGG - chr7_4:27021685-27021704 Msa1147640:CDS 35.0%
CTATAGATCAAAGCAGGAAA+GGG - chr7_4:27021686-27021705 Msa1147640:CDS 35.0%
TTTGTGTAGCAAAATCTCCA+TGG - chr7_4:27021716-27021735 Msa1147640:CDS 35.0%
TGCAGAATTTAGTGTAGTAG+AGG - chr7_4:27021834-27021853 Msa1147640:five_prime_UTR 35.0%
GCTTGAATACAGAAGGGATT+AGG + chr7_4:27019992-27020011 None:intergenic 40.0%
GTTGACAGCTTGAATACAGA+AGG + chr7_4:27019999-27020018 None:intergenic 40.0%
!! AGAGTGAAGAGATGGGTTTT+GGG + chr7_4:27020050-27020069 None:intergenic 40.0%
!! AAGAGTGAAGAGATGGGTTT+TGG + chr7_4:27020051-27020070 None:intergenic 40.0%
GAGATTGAAGAGTGAAGAGA+TGG + chr7_4:27020058-27020077 None:intergenic 40.0%
TTGAGGAATAGAGAGAGAGA+GGG + chr7_4:27020098-27020117 None:intergenic 40.0%
ATTGAGGAATAGAGAGAGAG+AGG + chr7_4:27020099-27020118 None:intergenic 40.0%
GTTGTGATGGTTGTAGATTG+AGG + chr7_4:27020115-27020134 None:intergenic 40.0%
CTCGTAGATTGATGTTGCAT+TGG + chr7_4:27020289-27020308 None:intergenic 40.0%
CAATGCAACATCAATCTACG+AGG - chr7_4:27020287-27020306 Msa1147640:intron 40.0%
TACGAGGTTCTATTCAACCA+TGG - chr7_4:27020303-27020322 Msa1147640:intron 40.0%
ATTCAACCATGGTCTTCCAA+TGG - chr7_4:27020314-27020333 Msa1147640:intron 40.0%
TTCAACCATGGTCTTCCAAT+GGG - chr7_4:27020315-27020334 Msa1147640:intron 40.0%
AAATTCCCATTGGAAGACCA+TGG + chr7_4:27020323-27020342 None:intergenic 40.0%
! TCCAATGGGAATTTTCCCAA+AGG - chr7_4:27020329-27020348 Msa1147640:intron 40.0%
! CCAATGGGAATTTTCCCAAA+GGG - chr7_4:27020330-27020349 Msa1147640:intron 40.0%
TGAACTCGTTTACACCCTTT+GGG + chr7_4:27020347-27020366 None:intergenic 40.0%
TTGAACTCGTTTACACCCTT+TGG + chr7_4:27020348-27020367 None:intergenic 40.0%
! GTTTCTGGGTCATTGAGTTA+TGG - chr7_4:27020453-27020472 Msa1147640:intron 40.0%
GGAAAGATCGATGCTTTAAC+CGG - chr7_4:27020474-27020493 Msa1147640:intron 40.0%
TACTCATAACAGGGAACCAA+AGG + chr7_4:27020521-27020540 None:intergenic 40.0%
CGACACGAATACTCATAACA+GGG + chr7_4:27020530-27020549 None:intergenic 40.0%
TCGACACGAATACTCATAAC+AGG + chr7_4:27020531-27020550 None:intergenic 40.0%
GAGAGAAAAAAGAGGAACCT+TGG + chr7_4:27020667-27020686 None:intergenic 40.0%
ATTTCCTAACGACCCAATTC+AGG + chr7_4:27020758-27020777 None:intergenic 40.0%
!!! GGACCTAGTTGTTTTGTTTC+TGG - chr7_4:27020784-27020803 Msa1147640:intron 40.0%
TAACCTATGCACATGGAGAA+TGG - chr7_4:27021233-27021252 Msa1147640:intron 40.0%
ATTCCATTCTCCATGTGCAT+AGG + chr7_4:27021239-27021258 None:intergenic 40.0%
!! GCAAGTATTTTGTTGTACGC+AGG - chr7_4:27021607-27021626 Msa1147640:CDS 40.0%
CAATCTGATTGGAGAATCCA+TGG + chr7_4:27021736-27021755 None:intergenic 40.0%
CAACAATAGGACCGAATCAT+TGG + chr7_4:27021789-27021808 None:intergenic 40.0%
TTCCAGAATCTGTTTCGCAT+TGG - chr7_4:27021896-27021915 Msa1147640:five_prime_UTR 40.0%
TTCCAATGCGAAACAGATTC+TGG + chr7_4:27021901-27021920 None:intergenic 40.0%
TCTGTTTCGCATTGGAAAGA+TGG - chr7_4:27021904-27021923 Msa1147640:five_prime_UTR 40.0%
GCTTGAATACAGAAGGGATT+AGG + chr7_4:27019992-27020011 None:intergenic 40.0%
GTTGACAGCTTGAATACAGA+AGG + chr7_4:27019999-27020018 None:intergenic 40.0%
!! AGAGTGAAGAGATGGGTTTT+GGG + chr7_4:27020050-27020069 None:intergenic 40.0%
!! AAGAGTGAAGAGATGGGTTT+TGG + chr7_4:27020051-27020070 None:intergenic 40.0%
GAGATTGAAGAGTGAAGAGA+TGG + chr7_4:27020058-27020077 None:intergenic 40.0%
TTGAGGAATAGAGAGAGAGA+GGG + chr7_4:27020098-27020117 None:intergenic 40.0%
ATTGAGGAATAGAGAGAGAG+AGG + chr7_4:27020099-27020118 None:intergenic 40.0%
GTTGTGATGGTTGTAGATTG+AGG + chr7_4:27020115-27020134 None:intergenic 40.0%
CTCGTAGATTGATGTTGCAT+TGG + chr7_4:27020289-27020308 None:intergenic 40.0%
CAATGCAACATCAATCTACG+AGG - chr7_4:27020287-27020306 Msa1147640:intron 40.0%
TACGAGGTTCTATTCAACCA+TGG - chr7_4:27020303-27020322 Msa1147640:intron 40.0%
ATTCAACCATGGTCTTCCAA+TGG - chr7_4:27020314-27020333 Msa1147640:intron 40.0%
TTCAACCATGGTCTTCCAAT+GGG - chr7_4:27020315-27020334 Msa1147640:intron 40.0%
AAATTCCCATTGGAAGACCA+TGG + chr7_4:27020323-27020342 None:intergenic 40.0%
! TCCAATGGGAATTTTCCCAA+AGG - chr7_4:27020329-27020348 Msa1147640:intron 40.0%
! CCAATGGGAATTTTCCCAAA+GGG - chr7_4:27020330-27020349 Msa1147640:intron 40.0%
TGAACTCGTTTACACCCTTT+GGG + chr7_4:27020347-27020366 None:intergenic 40.0%
TTGAACTCGTTTACACCCTT+TGG + chr7_4:27020348-27020367 None:intergenic 40.0%
! GTTTCTGGGTCATTGAGTTA+TGG - chr7_4:27020453-27020472 Msa1147640:intron 40.0%
GGAAAGATCGATGCTTTAAC+CGG - chr7_4:27020474-27020493 Msa1147640:intron 40.0%
TACTCATAACAGGGAACCAA+AGG + chr7_4:27020521-27020540 None:intergenic 40.0%
CGACACGAATACTCATAACA+GGG + chr7_4:27020530-27020549 None:intergenic 40.0%
TCGACACGAATACTCATAAC+AGG + chr7_4:27020531-27020550 None:intergenic 40.0%
GAGAGAAAAAAGAGGAACCT+TGG + chr7_4:27020667-27020686 None:intergenic 40.0%
ATTTCCTAACGACCCAATTC+AGG + chr7_4:27020758-27020777 None:intergenic 40.0%
!!! GGACCTAGTTGTTTTGTTTC+TGG - chr7_4:27020784-27020803 Msa1147640:intron 40.0%
TAACCTATGCACATGGAGAA+TGG - chr7_4:27021233-27021252 Msa1147640:intron 40.0%
ATTCCATTCTCCATGTGCAT+AGG + chr7_4:27021239-27021258 None:intergenic 40.0%
!! GCAAGTATTTTGTTGTACGC+AGG - chr7_4:27021607-27021626 Msa1147640:CDS 40.0%
CAATCTGATTGGAGAATCCA+TGG + chr7_4:27021736-27021755 None:intergenic 40.0%
CAACAATAGGACCGAATCAT+TGG + chr7_4:27021789-27021808 None:intergenic 40.0%
TTCCAGAATCTGTTTCGCAT+TGG - chr7_4:27021896-27021915 Msa1147640:five_prime_UTR 40.0%
TTCCAATGCGAAACAGATTC+TGG + chr7_4:27021901-27021920 None:intergenic 40.0%
TCTGTTTCGCATTGGAAAGA+TGG - chr7_4:27021904-27021923 Msa1147640:five_prime_UTR 40.0%
GAAGAGAAGAAGGAGAGAGA+GGG + chr7_4:27020021-27020040 None:intergenic 45.0%
TGAAGAGAAGAAGGAGAGAG+AGG + chr7_4:27020022-27020041 None:intergenic 45.0%
! TGGGATTGGTGAAGAGAAGA+AGG + chr7_4:27020031-27020050 None:intergenic 45.0%
!!! GAAGAGATGGGTTTTGGGAT+TGG + chr7_4:27020045-27020064 None:intergenic 45.0%
! CCCTTTGGGAAAATTCCCAT+TGG + chr7_4:27020333-27020352 None:intergenic 45.0%
GAGTTCAACGTTGACGAAGA+TGG - chr7_4:27020360-27020379 Msa1147640:intron 45.0%
TCGTACAGACGATGATTCCA+AGG - chr7_4:27020647-27020666 Msa1147640:intron 45.0%
GGTCCTTCACAATTTCCTGA+CGG - chr7_4:27020704-27020723 Msa1147640:intron 45.0%
AGTACCTGAATTGGGTCGTT+AGG - chr7_4:27020751-27020770 Msa1147640:intron 45.0%
GCACATGGAGAATGGAATGA+TGG - chr7_4:27021241-27021260 Msa1147640:intron 45.0%
TTGTTGTACGCAGGGTCTAT+CGG - chr7_4:27021616-27021635 Msa1147640:CDS 45.0%
CAGCCACTGTGTATTGGAAA+GGG - chr7_4:27021857-27021876 Msa1147640:five_prime_UTR 45.0%
CTTCCCTTTCCAATACACAG+TGG + chr7_4:27021863-27021882 None:intergenic 45.0%
GAAGAGAAGAAGGAGAGAGA+GGG + chr7_4:27020021-27020040 None:intergenic 45.0%
TGAAGAGAAGAAGGAGAGAG+AGG + chr7_4:27020022-27020041 None:intergenic 45.0%
! TGGGATTGGTGAAGAGAAGA+AGG + chr7_4:27020031-27020050 None:intergenic 45.0%
!!! GAAGAGATGGGTTTTGGGAT+TGG + chr7_4:27020045-27020064 None:intergenic 45.0%
! CCCTTTGGGAAAATTCCCAT+TGG + chr7_4:27020333-27020352 None:intergenic 45.0%
GAGTTCAACGTTGACGAAGA+TGG - chr7_4:27020360-27020379 Msa1147640:intron 45.0%
TCGTACAGACGATGATTCCA+AGG - chr7_4:27020647-27020666 Msa1147640:intron 45.0%
GGTCCTTCACAATTTCCTGA+CGG - chr7_4:27020704-27020723 Msa1147640:intron 45.0%
AGTACCTGAATTGGGTCGTT+AGG - chr7_4:27020751-27020770 Msa1147640:intron 45.0%
GCACATGGAGAATGGAATGA+TGG - chr7_4:27021241-27021260 Msa1147640:intron 45.0%
TTGTTGTACGCAGGGTCTAT+CGG - chr7_4:27021616-27021635 Msa1147640:CDS 45.0%
CAGCCACTGTGTATTGGAAA+GGG - chr7_4:27021857-27021876 Msa1147640:five_prime_UTR 45.0%
CTTCCCTTTCCAATACACAG+TGG + chr7_4:27021863-27021882 None:intergenic 45.0%
GTACGAACAGCGACACATTC+AGG + chr7_4:27020633-27020652 None:intergenic 50.0%
AATGTCAGAATTGGGCCGTC+AGG + chr7_4:27020722-27020741 None:intergenic 50.0%
GGGTCGTTAGGAAATCGTGA+AGG - chr7_4:27020763-27020782 Msa1147640:intron 50.0%
TAGAGGCAGCCACTGTGTAT+TGG - chr7_4:27021851-27021870 Msa1147640:five_prime_UTR 50.0%
GCAGCCACTGTGTATTGGAA+AGG - chr7_4:27021856-27021875 Msa1147640:five_prime_UTR 50.0%
GTACGAACAGCGACACATTC+AGG + chr7_4:27020633-27020652 None:intergenic 50.0%
AATGTCAGAATTGGGCCGTC+AGG + chr7_4:27020722-27020741 None:intergenic 50.0%
GGGTCGTTAGGAAATCGTGA+AGG - chr7_4:27020763-27020782 Msa1147640:intron 50.0%
TAGAGGCAGCCACTGTGTAT+TGG - chr7_4:27021851-27021870 Msa1147640:five_prime_UTR 50.0%
GCAGCCACTGTGTATTGGAA+AGG - chr7_4:27021856-27021875 Msa1147640:five_prime_UTR 50.0%
! CGTGTCGATGTTCCTAGCTC+TGG - chr7_4:27020543-27020562 Msa1147640:intron 55.0%
CGAACAGCGACACATTCAGG+AGG + chr7_4:27020630-27020649 None:intergenic 55.0%
GGGCCGTCAGGAAATTGTGA+AGG + chr7_4:27020710-27020729 None:intergenic 55.0%
GTACGCAGGGTCTATCGGAA+AGG - chr7_4:27021621-27021640 Msa1147640:CDS 55.0%
! CGTGTCGATGTTCCTAGCTC+TGG - chr7_4:27020543-27020562 Msa1147640:intron 55.0%
CGAACAGCGACACATTCAGG+AGG + chr7_4:27020630-27020649 None:intergenic 55.0%
GGGCCGTCAGGAAATTGTGA+AGG + chr7_4:27020710-27020729 None:intergenic 55.0%
GTACGCAGGGTCTATCGGAA+AGG - chr7_4:27021621-27021640 Msa1147640:CDS 55.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 27019988 27022002 27019988 ID=Msa1147640;Name=Msa1147640
chr7_4 mRNA 27019988 27022002 27019988 ID=Msa1147640-mRNA-1;Parent=Msa1147640;Name=Msa1147640-mRNA-1;_AED=0.19;_eAED=0.36;_QI=207|0|0.33|0.66|1|1|3|0|247
chr7_4 exon 27019988 27020163 27019988 ID=Msa1147640-mRNA-1:exon:6642;Parent=Msa1147640-mRNA-1
chr7_4 exon 27021029 27021122 27021029 ID=Msa1147640-mRNA-1:exon:6641;Parent=Msa1147640-mRNA-1
chr7_4 exon 27021322 27022002 27021322 ID=Msa1147640-mRNA-1:exon:6640;Parent=Msa1147640-mRNA-1
chr7_4 five_prime_UTR 27021796 27022002 27021796 ID=Msa1147640-mRNA-1:five_prime_utr;Parent=Msa1147640-mRNA-1
chr7_4 CDS 27021322 27021795 27021322 ID=Msa1147640-mRNA-1:cds;Parent=Msa1147640-mRNA-1
chr7_4 CDS 27021029 27021122 27021029 ID=Msa1147640-mRNA-1:cds;Parent=Msa1147640-mRNA-1
chr7_4 CDS 27019988 27020163 27019988 ID=Msa1147640-mRNA-1:cds;Parent=Msa1147640-mRNA-1
Gene Sequence

>Msa1147640

ATGAGAAAAAACATGTTTTCCTTCTCTTACATAACAACCATTCAATTCTTCCTTATTCTTTCATTTTCAACCCTTTCTTCTTCAACAAGTTCCAATGCAACATCAATCTACGAGGTTCTATTCAACCATGGTCTTCCAATGGGAATTTTCCCAAAGGGTGTAAACGAGTTCAACGTTGACGAAGATGGTAAATTCTGGGTACATTTAGATCAAGCATGTAACGCTAAATTTGAGAATGAATTGCATTATGATCGAAACGTTTCTGGGTCATTGAGTTATGGAAAGATCGATGCTTTAACCGGTTTAGAAGCTCAAGATCTTTTCCTTTGGTTCCCTGTTATGAGTATTCGTGTCGATGTTCCTAGCTCTGGTTTGATTTACTTTGATGTTGGTGCTGCTTATAAACAATTCTCTTTGTCCCTTTTTGAAACTCCTCCTGAATGTGTCGCTGTTCGTACAGACGATGATTCCAAGGAAGAATTGGGAAAAGGGTTGATTGAGATATTTGTCATGGTCAATTTCAATTGTTGTGTTGTTGTGATTGTGATTTTGTTTGGTGGAGGGTCTTCGTATAGTGCAGAATTTAGTGTAGTAGAGGCAGCCACTGTGTATTGGAAAGGGAAGCAACGAGATGAATTTCCAGAATCTGTTTCGCATTGGAAAGATGGAGCAAGAATAGAGCATTTTGTTGAAATGGATTCAGGTGACAAGTTTGCAAAGATTTTGTTGTATGTTGTATCATAA
Protein sequence

>Msa1147640

MRKNMFSFSYITTIQFFLILSFSTLSSSTSSNATSIYEVLFNHGLPMGIFPKGVNEFNVDEDGKFWVHLDQACNAKFENELHYDRNVSGSLSYGKIDALTGLEAQDLFLWFPVMSIRVDVPSSGLIYFDVGAAYKQFSLSLFETPPECVAVRTDDDSKEELGKGLIEIFVMVNFNCCVVVIVILFGGGSSYSAEFSVVEAATVYWKGKQRDEFPESVSHWKDGARIEHFVEMDSGDKFAKILLYVVS*