AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0584600


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0584600 MtrunA17_Chr4g0021961 96.364 110 4 0 1 110 1 110 3.41e-72 210
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAGCAACCCTCTTTATCTT+AGG 0.308528 4_2:-4370513 None:intergenic
GATAAAGAGGGTTGCTGTTA+AGG 0.330238 4_2:+4370518 Msa0584600:CDS
AGACAAGGATATGAGCTTCT+TGG 0.379115 4_2:+4370363 Msa0584600:CDS
CTAAAAGACCTAAGATAAAG+AGG 0.402553 4_2:+4370505 Msa0584600:CDS
CTAGCTCCCCTCTTCCTACT+AGG 0.437842 4_2:+4370622 Msa0584600:CDS
TATGAGCTTCTTGGTTTACA+AGG 0.464864 4_2:+4370372 Msa0584600:CDS
CCTACTAGGAAGATCCTTTG+AGG 0.470260 4_2:+4370636 Msa0584600:CDS
CCTCAAAGGATCTTCCTAGT+AGG 0.477106 4_2:-4370636 None:intergenic
GTTGCGAACGAGTGTGTTCA+TGG 0.491971 4_2:-4370319 None:intergenic
ATATCCTTGTCTCCTGCTGA+AGG 0.525798 4_2:-4370352 None:intergenic
GGATCTTCCTAGTAGGAAGA+GGG 0.550819 4_2:-4370629 None:intergenic
ACACCACTCTCACCTTCAGC+AGG 0.551528 4_2:+4370340 Msa0584600:CDS
AGGATCTTCCTAGTAGGAAG+AGG 0.558203 4_2:-4370630 None:intergenic
TCTCCTGCTGAAGGTGAGAG+TGG 0.568581 4_2:-4370343 None:intergenic
CTCACCTTCAGCAGGAGACA+AGG 0.589904 4_2:+4370348 Msa0584600:CDS
ATGAGCTTCTTGGTTTACAA+GGG 0.601511 4_2:+4370373 Msa0584600:CDS
TAAAAGACCTAAGATAAAGA+GGG 0.605297 4_2:+4370506 Msa0584600:CDS
GACGCAGAAGAGCCAAACAA+AGG 0.632309 4_2:+4370427 Msa0584600:CDS
GATCTTCCTAGTAGGAAGAG+GGG 0.633617 4_2:-4370628 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TAAAAGACCTAAGATAAAGA+GGG + chr4_2:4370506-4370525 Msa0584600:CDS 25.0%
! GAGAAAGATTTTCCTTTGTT+TGG - chr4_2:4370442-4370461 None:intergenic 30.0%
! CTCTTTATCTTAGGTCTTTT+AGG - chr4_2:4370507-4370526 None:intergenic 30.0%
CTAAAAGACCTAAGATAAAG+AGG + chr4_2:4370505-4370524 Msa0584600:CDS 30.0%
!!! GTTTTGTTGTTTATGAGAAC+TGG + chr4_2:4370561-4370580 Msa0584600:CDS 30.0%
TATGAGCTTCTTGGTTTACA+AGG + chr4_2:4370372-4370391 Msa0584600:CDS 35.0%
ATGAGCTTCTTGGTTTACAA+GGG + chr4_2:4370373-4370392 Msa0584600:CDS 35.0%
AGACAAGGATATGAGCTTCT+TGG + chr4_2:4370363-4370382 Msa0584600:CDS 40.0%
ACAGCAACCCTCTTTATCTT+AGG - chr4_2:4370516-4370535 None:intergenic 40.0%
! GATAAAGAGGGTTGCTGTTA+AGG + chr4_2:4370518-4370537 Msa0584600:CDS 40.0%
ATATCCTTGTCTCCTGCTGA+AGG - chr4_2:4370355-4370374 None:intergenic 45.0%
GATCTTCCTAGTAGGAAGAG+GGG - chr4_2:4370631-4370650 None:intergenic 45.0%
GGATCTTCCTAGTAGGAAGA+GGG - chr4_2:4370632-4370651 None:intergenic 45.0%
AGGATCTTCCTAGTAGGAAG+AGG - chr4_2:4370633-4370652 None:intergenic 45.0%
GACGCAGAAGAGCCAAACAA+AGG + chr4_2:4370427-4370446 Msa0584600:CDS 50.0%
ACACCACTCTCACCTTCAGC+AGG + chr4_2:4370340-4370359 Msa0584600:CDS 55.0%
TCTCCTGCTGAAGGTGAGAG+TGG - chr4_2:4370346-4370365 None:intergenic 55.0%
CTCACCTTCAGCAGGAGACA+AGG + chr4_2:4370348-4370367 Msa0584600:CDS 55.0%
CTAGCTCCCCTCTTCCTACT+AGG + chr4_2:4370622-4370641 Msa0584600:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 4370321 4370656 4370321 ID=Msa0584600;Name=Msa0584600
chr4_2 mRNA 4370321 4370656 4370321 ID=Msa0584600-mRNA-1;Parent=Msa0584600;Name=Msa0584600-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|111
chr4_2 exon 4370321 4370656 4370321 ID=Msa0584600-mRNA-1:exon:1131;Parent=Msa0584600-mRNA-1
chr4_2 CDS 4370321 4370656 4370321 ID=Msa0584600-mRNA-1:cds;Parent=Msa0584600-mRNA-1
Gene Sequence

>Msa0584600

ATGAACACACTCGTTCGCAACACCACTCTCACCTTCAGCAGGAGACAAGGATATGAGCTTCTTGGTTTACAAGGGAAAGAGAAAACACTCAAATCAGTCAGCTACAGACGCAGAAGAGCCAAACAAAGGAAAATCTTTCTCACAACATATAAACTATCTTCTTTGAATACTTTTGTAGAACAGCCTAAAAGACCTAAGATAAAGAGGGTTGCTGTTAAGGTTAAGAAAATTGTTAACTCTGTTTTGTTGTTTATGAGAACTGGTTCTTTTAGATCATGTAGTTCTAAGTCTGCTATTTCTGCTAGCTCCCCTCTTCCTACTAGGAAGATCCTTTGA

Protein sequence

>Msa0584600

MNTLVRNTTLTFSRRQGYELLGLQGKEKTLKSVSYRRRRAKQRKIFLTTYKLSSLNTFVEQPKRPKIKRVAVKVKKIVNSVLLFMRTGSFRSCSSKSAISASSPLPTRKIL*