AlfalfaGEDB | Msa1443620

M. sativa cultivar zhongmu-4 / Msa1443620

KEGG: -
GO: -
PFAM: PF07690.19

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1443620	A0A396JZ33	76.901	342	27	4	1	290	346	687	1.34e-158	508
Msa1443620	A0A396JZ33	81.818	341	11	4	1	290	940	1280	1.38e-166	530
Msa1443620	A0A396JZ33	83.264	239	16	3	1	215	1535	1773	1.32e-116	393
Msa1443620	A0A396JZ33	85.294	374	53	1	263	636	811	1182	0.0	628
Msa1443620	A0A396JZ33	93.646	362	23	0	279	640	231	592	0.0	668
Msa1443620	A0A396JZ33	94.231	364	21	0	279	642	1420	1783	0.0	675
Msa1443620	A0A396JZ33	94.872	78	4	0	213	290	14	91	2.35e-35	155

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001830	Msa1443620	0.835560	1.562761e-56	-8.615850e-47
Msa0004480	Msa1443620	0.856369	3.371545e-62	-8.615850e-47
Msa0005260	Msa1443620	0.810114	1.353705e-50	-8.615850e-47
Msa0006060	Msa1443620	0.829867	4.047267e-55	-8.615850e-47
Msa0009640	Msa1443620	0.815607	8.477541e-52	-8.615850e-47
Msa0011050	Msa1443620	0.810581	1.073654e-50	-8.615850e-47
Msa0026630	Msa1443620	0.827542	1.477452e-54	-8.615850e-47
Msa0052780	Msa1443620	0.814021	1.904076e-51	-8.615850e-47
Msa0059440	Msa1443620	0.824396	8.257948e-54	-8.615850e-47
Msa0059900	Msa1443620	0.829100	6.217838e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1443620	MtrunA17_Chr1g0210284	94.231	364	21	0	279	642	231	594	0.0	675
Msa1443620	MtrunA17_Chr1g0210284	83.264	239	16	3	1	215	346	584	4.30e-129	392
Msa1443620	MtrunA17_Chr1g0210284	98.718	78	1	0	213	290	14	91	1.21e-41	159
Msa1443620	MtrunA17_Chr1g0210274	93.681	364	23	0	279	642	231	594	0.0	672
Msa1443620	MtrunA17_Chr1g0210274	79.600	250	27	3	1	226	346	595	3.26e-128	390
Msa1443620	MtrunA17_Chr1g0210274	94.872	78	4	0	213	290	14	91	4.36e-40	155
Msa1443620	MtrunA17_Chr1g0210291	66.848	368	113	4	279	641	230	593	9.01e-164	482
Msa1443620	MtrunA17_Chr1g0210291	64.542	251	57	4	1	219	344	594	2.72e-97	310
Msa1443620	MtrunA17_Chr1g0210291	69.620	79	24	0	212	290	12	90	2.03e-29	123
Msa1443620	MtrunA17_Chr7g0264861	60.256	312	117	2	329	635	295	604	1.02e-125	385

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1443620	AT2G39210.1	65.363	358	111	2	291	635	238	595	2.02e-159	471
Msa1443620	AT2G39210.1	63.964	222	56	3	1	198	345	566	5.79e-88	286
Msa1443620	AT2G39210.1	77.632	76	17	0	215	290	11	86	5.64e-32	131
Msa1443620	AT2G28120.1	57.012	328	138	2	291	617	234	559	3.12e-125	382
Msa1443620	AT2G28120.1	59.375	224	67	3	1	200	336	559	8.07e-77	256
Msa1443620	AT2G28120.1	70.833	72	21	0	219	290	11	82	7.26e-27	115
Msa1443620	AT2G34355.2	37.037	270	145	3	352	611	116	370	2.78e-57	198
Msa1443620	AT2G34355.2	38.725	204	113	3	3	194	167	370	2.14e-39	149
Msa1443620	AT1G74780.1	42.128	235	124	1	381	615	311	533	2.85e-56	199
Msa1443620	AT1G74780.1	37.500	208	118	3	3	198	326	533	5.20e-37	145

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Find 109 sgRNAs with CRISPR-Local

Find 291 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGTTCTGAAACAAATC+CGG	0.495163	tig0030488:+26157	None:intergenic
AAAGAAAGCATAAGAGGACG+AGG	0.591288	tig0030488:+26116	None:intergenic
AAAGAACGTGGAAAAGACAG+AGG	0.686229	tig0030488:-22087	Msa1443620:CDS
AAAGCATAGAATAGAAGGGC+AGG	0.553260	tig0030488:-25823	Msa1443620:CDS
AAAGTAAGCATAAGAGGACG+AGG	0.591288	tig0030488:+22569	None:intergenic
AACACATCTTCCCACCACTT+TGG	0.442991	tig0030488:+22851	None:intergenic
AACTCTCTTTGTCTCATGTG+TGG	0.452502	tig0030488:-22546	Msa1443620:CDS
AAGAAACAATTGGAAGCACT+TGG	0.542138	tig0030488:-22299	Msa1443620:CDS
AAGAAACAATTGGAAGCACT+TGG	0.542138	tig0030488:-25846	Msa1443620:CDS
AAGAATAGTAGTTTCACACA+GGG	0.699884	tig0030488:-23079	Msa1443620:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAAGCACATATAAGCCAAT+TGG	+	tig0030488:22457-22476	None:intergenic	30.0%
CAAAGAAGCTAAGAAACAAT+TGG	-	tig0030488:22504-22523	Msa1443620:CDS	30.0%
GTGACATATACAAGAGATAT+AGG	-	tig0030488:22680-22699	Msa1443620:CDS	30.0%
GCTATAAATGTGCAAAATGT+TGG	+	tig0030488:23377-23396	None:intergenic	30.0%
TAGAATCATAATATCCACCA+TGG	-	tig0030488:23447-23466	Msa1443620:intron	30.0%
AATATCATGTTGAGAATCCA+TGG	+	tig0030488:23467-23486	None:intergenic	30.0%
TAGAATCATAATATCCACCA+TGG	-	tig0030488:23447-23466	Msa1443620:intron	30.0%
ACAACAAAGATAGAATGAAG+AGG	+	tig0030488:23546-23565	None:intergenic	30.0%
CCCCATAATATTATTTATCG+TGG	+	tig0030488:23669-23688	None:intergenic	30.0%
TAGTAAACCTTTCACTAGAA+AGG	+	tig0030488:23710-23729	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0030488	gene	22043	26339	22043	ID=Msa1443620;Name=Msa1443620
tig0030488	mRNA	22043	26339	22043	ID=Msa1443620-mRNA-1;Parent=Msa1443620;Name=Msa1443620-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0\|0\|0\|1\|0.2\|0.16\|6\|0\|642
tig0030488	exon	26210	26339	26210	ID=Msa1443620-mRNA-1:exon:14026;Parent=Msa1443620-mRNA-1
tig0030488	exon	25954	26191	25954	ID=Msa1443620-mRNA-1:exon:14025;Parent=Msa1443620-mRNA-1
tig0030488	exon	25808	25938	25808	ID=Msa1443620-mRNA-1:exon:14024;Parent=Msa1443620-mRNA-1
tig0030488	exon	25623	25768	25623	ID=Msa1443620-mRNA-1:exon:14023;Parent=Msa1443620-mRNA-1
tig0030488	exon	23555	23779	23555	ID=Msa1443620-mRNA-1:exon:14022;Parent=Msa1443620-mRNA-1
tig0030488	exon	22043	23101	22043	ID=Msa1443620-mRNA-1:exon:14021;Parent=Msa1443620-mRNA-1
tig0030488	CDS	26210	26339	26210	ID=Msa1443620-mRNA-1:cds;Parent=Msa1443620-mRNA-1
tig0030488	CDS	25954	26191	25954	ID=Msa1443620-mRNA-1:cds;Parent=Msa1443620-mRNA-1
tig0030488	CDS	25808	25938	25808	ID=Msa1443620-mRNA-1:cds;Parent=Msa1443620-mRNA-1
tig0030488	CDS	25623	25768	25623	ID=Msa1443620-mRNA-1:cds;Parent=Msa1443620-mRNA-1
tig0030488	CDS	23555	23779	23555	ID=Msa1443620-mRNA-1:cds;Parent=Msa1443620-mRNA-1
tig0030488	CDS	22043	23101	22043	ID=Msa1443620-mRNA-1:cds;Parent=Msa1443620-mRNA-1

Gene Sequence

>Msa1443620

ATGTTGATACTATTTGTGGCATGTATTTGTGGTATTGGTGGATCATTGACAGCAGTTGATAATCTTGGTCAGATAGGAACATCACTTAGATATCCAAAGAAAAGCATAAGCACTTTTGTGTCTTTAGTAAGACGTGTTTTCTCCGGATTTGTTTCAGAACATTTTCTAACAAAGTACAGATTTCCTCGTCCTCTTATGCTTTCTTTGACTCTCTTTGTCTCGTGTGTGGGACACCTTTTGATAGCATTTGGTGTGCCAAAGGGTCTCTATGCAGCTTCAGTGATTATCGGATTTTGTTTTGGTGCTCAATGGCCATTACTTTTTGCCATTATATCAGAACTTTTTGGACTTAAATATTATGCAACTTTTATAACAAGTCCAATTGGCTTATATGTGCTTAATGTGAAGATCACTGGTCATTTGTATGACAAAGAAGCTAAGAAACAATTGGAAGCACTTGGAAAGCATAGAATAGAAGGGCAGGAATTGAATTGTGTTGCTGCAACTTTCTTTGGTGCTGTTGTTAACCTTATTTTGGTGGCTAGGACAAGAAAGTTTTACAAAGGTGACATATACAAGAGATATAGGGAAGAAGCTGAAATGGTGGAGGTAAAGAACGAGGGAAAAACAGAAGAAGATGACAAAAGTCTCACTGTCCAATTCATCACCGGACGGTGGTTCGTCATTTTCGCCTCCTTCCTAATCATGGCAGCCGCCGGAGCCACCTACATGTTCGGTCTATACTCCGGTGACATAAAAACAACACTTGGTTATGACCAAAGTACACTAAACCTCCTTAGTTTTTTCAAAGATTTTGGTAGCAACGTTGGTGTCCTTGCAGGACTAGTCAACGAACTCTATCCACCTTGGAAGAATAGTAGTTTCACACAGGGTGAGTTTGGTTTAAGTGCTGCTGTGGTTATTTTCTTGCTTTTTCTTCCACTTGCCGTTGTTTTCATTGAAGAGAACAAGATTTGGCAGAGTAAAAAACTAGCTTTGGTTGATCCTTCACCAGTGCAAATAGTAACAGAAGGTGAAACTGTAACTGAAACAGAAAAAGTCAACTCTGCTGTTTCAGTTTCAGCTCCAAAAAAAGATCCAAAGTGGTGGGAAGATGTGTTCAATCCACCAGCCAGAGGTGATGATTATACAATACTTCAAGCACTTTTCAGCATAGATATGTTGATACTATTTGTGGCATGTATTTGTGGTGTTGGTGGAACTTTGACAGCAATTGATAATCTTGGTCAGATAGGAACATCACTTAGATATCCAAAGAAAAGCATAAGCACTTTTGTGTCTTTAGTAAGCATTTGGAATTATCTAGGACGTGTTTTCTCAGGATTTGTTTCTGAACATTTTCTAACGAAGTACAGATTTCCTCGTCCTCTTATGCTTACTTTAACTCTCTTTGTCTCATGTGTGGGACACCTTCTGATAGCATTTGATGTGCCAGAGGGTCTCTACGTAGCTTCAGTAATAATTGGATTTTGTTTTGGTGCTCAATGGCCATTACTTTTTGCCATTATATCAGAACTTTTTGGGCTAAAATATTATGCAACTTTGTATAATTTTGGAAGTGTAGCAAGTCCACTTGGGTTATATGTGCTTAATGTGAAGATCACTGGTCATTTGTATGACAAAGAAGCTAAGAAACAATTGGAAGCACTTGGAAAGCATAGAATAGAAGGGCGTGAATTGAATTGTGTTGGTGTTGATTGTTTCAAGTTATCCTTCATTATTATTACCGCTGCAACATTTTTTGGTGCTGTTGTTTCACTTATTTTGGTGGCTAGGACAAGAAAGTTTTACAAAGGTGACATATACAAGAGGTACAGAGAAGAAGCTGAAATGGTGGAGGTAAAGAACGTGGAAAAGACAGAGGAAGATGACAAAGTTGGTCAACAGCAGCATGTGGTGACAACTTAG

Protein sequence

>Msa1443620

MLILFVACICGIGGSLTAVDNLGQIGTSLRYPKKSISTFVSLVRRVFSGFVSEHFLTKYRFPRPLMLSLTLFVSCVGHLLIAFGVPKGLYAASVIIGFCFGAQWPLLFAIISELFGLKYYATFITSPIGLYVLNVKITGHLYDKEAKKQLEALGKHRIEGQELNCVAATFFGAVVNLILVARTRKFYKGDIYKRYREEAEMVEVKNEGKTEEDDKSLTVQFITGRWFVIFASFLIMAAAGATYMFGLYSGDIKTTLGYDQSTLNLLSFFKDFGSNVGVLAGLVNELYPPWKNSSFTQGEFGLSAAVVIFLLFLPLAVVFIEENKIWQSKKLALVDPSPVQIVTEGETVTETEKVNSAVSVSAPKKDPKWWEDVFNPPARGDDYTILQALFSIDMLILFVACICGVGGTLTAIDNLGQIGTSLRYPKKSISTFVSLVSIWNYLGRVFSGFVSEHFLTKYRFPRPLMLTLTLFVSCVGHLLIAFDVPEGLYVASVIIGFCFGAQWPLLFAIISELFGLKYYATLYNFGSVASPLGLYVLNVKITGHLYDKEAKKQLEALGKHRIEGRELNCVGVDCFKLSFIIITAATFFGAVVSLILVARTRKFYKGDIYKRYREEAEMVEVKNVEKTEEDDKVGQQQHVVTT*