AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1205410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1205410 MtrunA17_Chr4g0054491 97.087 103 3 0 1 103 1 103 3.34e-73 212
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1205410 AT4G37830.1 71.154 104 27 3 1 103 1 102 1.36e-48 150
Msa1205410 AT4G37830.2 63.462 104 25 4 1 103 1 92 2.32e-38 124
Msa1205410 AT4G37830.3 67.532 77 23 2 1 77 1 75 3.14e-29 103

Find 60 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGAACTTCTCGGTCTATTT+TGG 0.117274 8_1:+61709583 None:intergenic
TTATTGCGGATATGCAAATA+TGG 0.260738 8_1:+61707400 None:intergenic
CTTTGGCTGTAGCCTCAAAC+AGG 0.332714 8_1:-61706385 Msa1205410:three_prime_UTR
CAAACATCATATTGTTTGTT+GGG 0.352046 8_1:+61706256 None:intergenic
TCATGTTCTCTTCTTTCTCA+TGG 0.360031 8_1:-61706298 Msa1205410:three_prime_UTR
TTGATTTCAAATAGCCCATC+TGG 0.366613 8_1:+61706471 None:intergenic
ACAAACATCATATTGTTTGT+TGG 0.382436 8_1:+61706255 None:intergenic
ATATTTGCATATCCGCAATA+AGG 0.383345 8_1:-61707398 Msa1205410:CDS
ATATGTAATCTTCTCCCATT+TGG 0.387052 8_1:+61708604 None:intergenic
TGGGCACTAATGTCTTCCTT+TGG 0.396894 8_1:-61706402 Msa1205410:three_prime_UTR
TGGGAGAAGATTACATATCT+AGG 0.412735 8_1:-61708599 Msa1205410:CDS
TATTTCGAAGAAGAGTAAAA+TGG 0.414452 8_1:-61709498 Msa1205410:exon
CTAGGCATTGTTTCATGTAC+CGG 0.435132 8_1:-61708581 Msa1205410:CDS
AGAAAAGTTTCGCTTGGGAG+CGG 0.441949 8_1:+61709409 None:intergenic
TATAAGATGAAACGGCCAAA+TGG 0.446760 8_1:-61708619 Msa1205410:intron
GGAGGAAGAAAAGTTTCGCT+TGG 0.451508 8_1:+61709403 None:intergenic
GCGACGACGTTGATGCGATC+TGG 0.452055 8_1:-61709476 Msa1205410:CDS
CTCCTTTGTATAAGATGAAA+CGG 0.453752 8_1:-61708627 Msa1205410:intron
GTTTCGCTTGGGAGCGGGAG+AGG 0.459261 8_1:+61709415 None:intergenic
GTCCTCCGACGAGGAGGCTC+CGG 0.462096 8_1:-61709440 Msa1205410:CDS
GAGGAAGAAAAGTTTCGCTT+GGG 0.467177 8_1:+61709404 None:intergenic
GAAAAGTTTCGCTTGGGAGC+GGG 0.478655 8_1:+61709410 None:intergenic
TCACTACTTGGTGGATAAAG+TGG 0.483260 8_1:-61706105 Msa1205410:three_prime_UTR
TAGAGTATAAACAGCTAAAC+CGG 0.486251 8_1:+61708562 None:intergenic
AGCACCACTAGGAATATTAG+TGG 0.487416 8_1:-61706443 Msa1205410:exon
AGCTGTTTATACTCTATCAA+AGG 0.488901 8_1:-61708555 Msa1205410:CDS
CTTGGGAGCGGGAGAGGCAC+CGG 0.489102 8_1:+61709421 None:intergenic
GGTTTAATGAAGGTGGTTTG+TGG 0.489309 8_1:-61706422 Msa1205410:three_prime_UTR
TTTGATTCATCACTACTTGG+TGG 0.490515 8_1:-61706114 Msa1205410:three_prime_UTR
GCTGTTTATACTCTATCAAA+GGG 0.495796 8_1:-61708554 Msa1205410:CDS
AGGTTTGATTCATCACTACT+TGG 0.501061 8_1:-61706117 Msa1205410:three_prime_UTR
GAATATTAGTGGTTTAATGA+AGG 0.504937 8_1:-61706432 Msa1205410:three_prime_UTR
AACTGCAATAACCCTGTTTG+AGG 0.513422 8_1:+61706373 None:intergenic
ATAAGATGAAACGGCCAAAT+GGG 0.513873 8_1:-61708618 Msa1205410:intron
TATTAGTGGTTTAATGAAGG+TGG 0.519985 8_1:-61706429 Msa1205410:three_prime_UTR
GTTTAATGAAGGTGGTTTGT+GGG 0.527505 8_1:-61706421 Msa1205410:three_prime_UTR
TATTGCGGATATGCAAATAT+GGG 0.531200 8_1:+61707401 None:intergenic
TGATGATAAACAGGTCCAGA+TGG 0.532448 8_1:-61706486 Msa1205410:intron
GAGGTTCATCATAGTGGTGG+TGG 0.540593 8_1:+61708531 None:intergenic
CTGTTTGAGGCTACAGCCAA+AGG 0.572052 8_1:+61706386 None:intergenic
CTTCGCAACGTCCTCCGACG+AGG 0.587981 8_1:-61709449 Msa1205410:CDS
TGTGATTTAGAACTTGCACT+TGG 0.591187 8_1:-61706332 Msa1205410:three_prime_UTR
CACTACTTGGTGGATAAAGT+GGG 0.593788 8_1:-61706104 Msa1205410:three_prime_UTR
CACCGGAGCCTCCTCGTCGG+AGG 0.600406 8_1:+61709438 None:intergenic
GATGATAAACAGGTCCAGAT+GGG 0.601053 8_1:-61706485 Msa1205410:intron
CCCATGGGAATTCCTTATTG+CGG 0.601121 8_1:+61707386 None:intergenic
TTACAGGAGGTTCATCATAG+TGG 0.602947 8_1:+61708525 None:intergenic
AATCAAGAACGAGCACCACT+AGG 0.603430 8_1:-61706454 Msa1205410:exon
TCCGCAATAAGGAATTCCCA+TGG 0.603749 8_1:-61707387 Msa1205410:CDS
CCGCAATAAGGAATTCCCAT+GGG 0.606158 8_1:-61707386 Msa1205410:CDS
CGCAACGTCCTCCGACGAGG+AGG 0.607912 8_1:-61709446 Msa1205410:CDS
ACACAGCCATTATGAAACAC+AGG 0.639520 8_1:+61706168 None:intergenic
TGCATCATCGTGATGACCAG+AGG 0.640046 8_1:+61709382 None:intergenic
TTTGGCTGTAGCCTCAAACA+GGG 0.644011 8_1:-61706384 Msa1205410:three_prime_UTR
CAGGAGGTTCATCATAGTGG+TGG 0.646029 8_1:+61708528 None:intergenic
GTGAATGACACATACCCCAT+GGG 0.647797 8_1:+61707371 None:intergenic
AGGCACCGGAGCCTCCTCGT+CGG 0.660173 8_1:+61709435 None:intergenic
ATCATCGTGATGACCAGAGG+AGG 0.665684 8_1:+61709385 None:intergenic
TAAACCACTAATATTCCTAG+TGG 0.676016 8_1:+61706439 None:intergenic
CGCAATAAGGAATTCCCATG+GGG 0.679540 8_1:-61707385 Msa1205410:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTATAATTTATATTAAGTAT+TGG + chr8_1:61706842-61706861 None:intergenic 10.0%
!! AATATTAAAACTATAAGTAA+TGG - chr8_1:61707448-61707467 Msa1205410:intron 10.0%
!! TCAATTTATCAATATGAATA+TGG - chr8_1:61706428-61706447 Msa1205410:three_prime_UTR 15.0%
!! CAATTTATCAATATGAATAT+GGG - chr8_1:61706429-61706448 Msa1205410:three_prime_UTR 15.0%
!! TTATTCATTCTTGTAATAAT+GGG + chr8_1:61708007-61708026 None:intergenic 15.0%
!!! TTTTCTGTGTTTTTTACAAA+TGG + chr8_1:61706159-61706178 None:intergenic 20.0%
!!! CTCTTATATTTGGTTAATTT+TGG - chr8_1:61706395-61706414 Msa1205410:three_prime_UTR 20.0%
!!! TCATTTTATTAGAAAAGAAC+AGG + chr8_1:61707427-61707446 None:intergenic 20.0%
!! TGGAAGTTTATAAATAAAAG+AGG - chr8_1:61707468-61707487 Msa1205410:intron 20.0%
!!! TTGATCTTTTATTCAGTTAA+AGG + chr8_1:61707509-61707528 None:intergenic 20.0%
!! ATAAATAGAATAAACACTGT+CGG - chr8_1:61707684-61707703 Msa1205410:intron 20.0%
!! GAACTATTAAAATTCACATA+TGG + chr8_1:61707820-61707839 None:intergenic 20.0%
!! CTTATTCATTCTTGTAATAA+TGG + chr8_1:61708008-61708027 None:intergenic 20.0%
!! TTACAAGAATGAATAAGAAA+AGG - chr8_1:61708011-61708030 Msa1205410:intron 20.0%
!!! AATCTACTGTATTTATTTTC+CGG + chr8_1:61708092-61708111 None:intergenic 20.0%
!! ACAAGATAATTTGAAAACAA+AGG + chr8_1:61709145-61709164 None:intergenic 20.0%
!!! TTTTATTTCAAGTCTTGTTT+AGG - chr8_1:61709454-61709473 Msa1205410:CDS 20.0%
!! AAGTTACCAAAAATATAACT+AGG - chr8_1:61709549-61709568 Msa1205410:five_prime_UTR 20.0%
!!! ATCAAACCTAGTTATATTTT+TGG + chr8_1:61709558-61709577 None:intergenic 20.0%
! TATTTCGAAGAAGAGTAAAA+TGG - chr8_1:61706188-61706207 Msa1205410:three_prime_UTR 25.0%
!! TACTCCTTTTACATTCTTTT+AGG - chr8_1:61706333-61706352 Msa1205410:three_prime_UTR 25.0%
!! ACTCCTTTTACATTCTTTTA+GGG - chr8_1:61706334-61706353 Msa1205410:three_prime_UTR 25.0%
! AAACCCTAAAAGAATGTAAA+AGG + chr8_1:61706340-61706359 None:intergenic 25.0%
! ATCAATATGAATATGGGAAA+TGG - chr8_1:61706435-61706454 Msa1205410:three_prime_UTR 25.0%
! AATGAATTTGTGCTTTGATT+CGG - chr8_1:61706568-61706587 Msa1205410:intron 25.0%
! AAATGACCAAATCGAATTTA+TGG - chr8_1:61706705-61706724 Msa1205410:intron 25.0%
! AATGACCAAATCGAATTTAT+GGG - chr8_1:61706706-61706725 Msa1205410:intron 25.0%
! AAATACAAATGCAAACTTAG+TGG + chr8_1:61706768-61706787 None:intergenic 25.0%
!!! TATCATCGAAGGTTATTTTT+TGG - chr8_1:61706799-61706818 Msa1205410:intron 25.0%
! AGCATGTAAAGAAGAATTTA+AGG + chr8_1:61707007-61707026 None:intergenic 25.0%
! TAGTGAAATTTCAACATGAA+AGG + chr8_1:61707223-61707242 None:intergenic 25.0%
!! TTTTATTCCTTTTCATTTCC+AGG - chr8_1:61707713-61707732 Msa1205410:intron 25.0%
!! TTTATTCCTTTTCATTTCCA+GGG - chr8_1:61707714-61707733 Msa1205410:intron 25.0%
! CATGAATGACTTAACTTTAT+GGG - chr8_1:61707926-61707945 Msa1205410:intron 25.0%
! AGAATGAATAAGAAAAGGAA+TGG - chr8_1:61708016-61708035 Msa1205410:intron 25.0%
! CATAGACAAATATGAATGTT+CGG + chr8_1:61708066-61708085 None:intergenic 25.0%
!!! ATCTACTGTATTTATTTTCC+GGG + chr8_1:61708091-61708110 None:intergenic 25.0%
! TCTAATCAATCCAACAAATT+TGG + chr8_1:61708401-61708420 None:intergenic 25.0%
! AGAAATAAGTAATATTGCAG+TGG + chr8_1:61708559-61708578 None:intergenic 25.0%
! TAGTCTAGTTGCAATTAATT+GGG - chr8_1:61708595-61708614 Msa1205410:CDS 25.0%
! AATAACAATTCCTCAATTTG+TGG + chr8_1:61708712-61708731 None:intergenic 25.0%
!!! CTCTTTTTTGTTTACATGTT+AGG - chr8_1:61708746-61708765 Msa1205410:intron 25.0%
!!! ATTTATCCATAAAGTACTGT+TGG + chr8_1:61708851-61708870 None:intergenic 25.0%
!!! AATTTTGATAGACAAGTCTT+TGG - chr8_1:61708868-61708887 Msa1205410:intron 25.0%
! GAAAATCGTTAATTATGTTG+AGG + chr8_1:61708901-61708920 None:intergenic 25.0%
! AAAATGACAAACAATATGCA+CGG + chr8_1:61708997-61709016 None:intergenic 25.0%
!! ATTTCTCTTTGATGATAAAC+AGG - chr8_1:61709191-61709210 Msa1205410:intron 25.0%
! GAATATTAGTGGTTTAATGA+AGG - chr8_1:61709254-61709273 Msa1205410:intron 25.0%
! CAAACATCATATTGTTTGTT+GGG + chr8_1:61709433-61709452 None:intergenic 25.0%
! ACAAACATCATATTGTTTGT+TGG + chr8_1:61709434-61709453 None:intergenic 25.0%
AGAAGAAAAAACGAACTTCT+CGG + chr8_1:61706116-61706135 None:intergenic 30.0%
CGATGTTTAGTATTCAGAAA+TGG - chr8_1:61706628-61706647 Msa1205410:intron 30.0%
!! AGTGGATTCAACAAGATTTT+GGG + chr8_1:61706750-61706769 None:intergenic 30.0%
!!! TAGTGGATTCAACAAGATTT+TGG + chr8_1:61706751-61706770 None:intergenic 30.0%
!! ATAGATCTTGTAGCTGTTTT+AGG - chr8_1:61706950-61706969 Msa1205410:intron 30.0%
CTCCTTTGTATAAGATGAAA+CGG - chr8_1:61707059-61707078 Msa1205410:intron 30.0%
ATATGTAATCTTCTCCCATT+TGG + chr8_1:61707085-61707104 None:intergenic 30.0%
TAGAGTATAAACAGCTAAAC+CGG + chr8_1:61707127-61707146 None:intergenic 30.0%
AGCTGTTTATACTCTATCAA+AGG - chr8_1:61707131-61707150 Msa1205410:intron 30.0%
GCTGTTTATACTCTATCAAA+GGG - chr8_1:61707132-61707151 Msa1205410:intron 30.0%
TATAACAGAAAACTTACAGG+AGG + chr8_1:61707177-61707196 None:intergenic 30.0%
CCATATAACAGAAAACTTAC+AGG + chr8_1:61707180-61707199 None:intergenic 30.0%
! CCTGTAAGTTTTCTGTTATA+TGG - chr8_1:61707177-61707196 Msa1205410:intron 30.0%
! GATATGTATTCATTTTCTCC+TGG - chr8_1:61707359-61707378 Msa1205410:intron 30.0%
!!! ATTGTTTTTACTGTCTCTGA+TGG + chr8_1:61707573-61707592 None:intergenic 30.0%
! AGAGTACATCAATATTTCTG+CGG + chr8_1:61707637-61707656 None:intergenic 30.0%
TTTGTTACAGTCTATATAGC+TGG + chr8_1:61707873-61707892 None:intergenic 30.0%
GCATGAATGACTTAACTTTA+TGG - chr8_1:61707925-61707944 Msa1205410:intron 30.0%
! CTTAACTTTATGGGAAGTTT+TGG - chr8_1:61707935-61707954 Msa1205410:intron 30.0%
!!! TTATGGGAAGTTTTGGAATT+TGG - chr8_1:61707942-61707961 Msa1205410:intron 30.0%
AAAAGGAATGGCTTAAAATG+AGG - chr8_1:61708028-61708047 Msa1205410:intron 30.0%
AATATGAATGTTCGGATACA+TGG + chr8_1:61708058-61708077 None:intergenic 30.0%
TTCATATTTGTCTATGTACC+CGG - chr8_1:61708070-61708089 Msa1205410:intron 30.0%
GAAACAAATGACTACACATA+AGG + chr8_1:61708146-61708165 None:intergenic 30.0%
TTTCCTATAATACACCTTTC+AGG - chr8_1:61708162-61708181 Msa1205410:intron 30.0%
AACTCTTCTATTTATGATGC+AGG - chr8_1:61708258-61708277 Msa1205410:intron 30.0%
TATTGCGGATATGCAAATAT+GGG + chr8_1:61708288-61708307 None:intergenic 30.0%
TTATTGCGGATATGCAAATA+TGG + chr8_1:61708289-61708308 None:intergenic 30.0%
ATATTTGCATATCCGCAATA+AGG - chr8_1:61708288-61708307 Msa1205410:intron 30.0%
AATCAATCCAACAAATTTGG+TGG + chr8_1:61708398-61708417 None:intergenic 30.0%
! AATTTAGAATGCAGCAATGA+AGG + chr8_1:61708435-61708454 None:intergenic 30.0%
! CATTTGTTAAGCACAAGAAT+AGG - chr8_1:61708496-61708515 Msa1205410:intron 30.0%
CTAGTCTAGTTGCAATTAAT+TGG - chr8_1:61708594-61708613 Msa1205410:CDS 30.0%
TTGGGATAAGCTTCATATTA+TGG - chr8_1:61708613-61708632 Msa1205410:CDS 30.0%
CTTCATATTATGGTATCTAC+CGG - chr8_1:61708623-61708642 Msa1205410:intron 30.0%
CAAATACCAACAGTACTTTA+TGG - chr8_1:61708842-61708861 Msa1205410:intron 30.0%
! AAAATGGGTGATTGAAGTTT+TGG + chr8_1:61708957-61708976 None:intergenic 30.0%
!!! TGTCATTTTTTGTGCTCATT+TGG - chr8_1:61709008-61709027 Msa1205410:intron 30.0%
! AAAGGCATACACAATATCAA+TGG + chr8_1:61709127-61709146 None:intergenic 30.0%
TAAACCACTAATATTCCTAG+TGG + chr8_1:61709250-61709269 None:intergenic 30.0%
TATTAGTGGTTTAATGAAGG+TGG - chr8_1:61709257-61709276 Msa1205410:intron 30.0%
GAAAAACCTGTGTTTCATAA+TGG - chr8_1:61709512-61709531 Msa1205410:five_prime_UTR 30.0%
TGAATATGGGAAATGGTATG+TGG - chr8_1:61706442-61706461 Msa1205410:exon 35.0%
!! TGTGGATTTTCATTTTGACC+TGG - chr8_1:61706460-61706479 Msa1205410:CDS 35.0%
ATTAGGCAAAATCACTCTCA+TGG - chr8_1:61706668-61706687 Msa1205410:intron 35.0%
CTTGTCCCATAAATTCGATT+TGG + chr8_1:61706714-61706733 None:intergenic 35.0%
TATAAGATGAAACGGCCAAA+TGG - chr8_1:61707067-61707086 Msa1205410:intron 35.0%
ATAAGATGAAACGGCCAAAT+GGG - chr8_1:61707068-61707087 Msa1205410:intron 35.0%
TGGGAGAAGATTACATATCT+AGG - chr8_1:61707087-61707106 Msa1205410:intron 35.0%
! TAGCTTCTTTGACTTCGAAA+AGG - chr8_1:61707601-61707620 Msa1205410:intron 35.0%
! TATGAGTCATTTGTTGCCTT+GGG + chr8_1:61707667-61707686 None:intergenic 35.0%
! TTATGAGTCATTTGTTGCCT+TGG + chr8_1:61707668-61707687 None:intergenic 35.0%
AAAAAGCCCTGGAAATGAAA+AGG + chr8_1:61707723-61707742 None:intergenic 35.0%
TGATTATGACTAAAAAGCCC+TGG + chr8_1:61707734-61707753 None:intergenic 35.0%
!!! AAGATATTGCTTTTTGCCGT+TGG - chr8_1:61707755-61707774 Msa1205410:intron 35.0%
!!! AGATATTGCTTTTTGCCGTT+GGG - chr8_1:61707756-61707775 Msa1205410:intron 35.0%
!! GCAAAGCATTGATGCTTTAT+AGG + chr8_1:61707851-61707870 None:intergenic 35.0%
TCATAGAATAACTTCTGAGC+TGG + chr8_1:61708115-61708134 None:intergenic 35.0%
! AACAAATTTGGTGGAATGGT+TGG + chr8_1:61708389-61708408 None:intergenic 35.0%
AACCATTCCACCAAATTTGT+TGG - chr8_1:61708388-61708407 Msa1205410:intron 35.0%
! ATCCAACAAATTTGGTGGAA+TGG + chr8_1:61708393-61708412 None:intergenic 35.0%
GAATAGGCATATGTCATAGT+TGG - chr8_1:61708512-61708531 Msa1205410:intron 35.0%
! GTCATAGTTGGTTGGTATAA+TGG - chr8_1:61708524-61708543 Msa1205410:intron 35.0%
!! CTCCATTTTTCAGAGTTACA+AGG - chr8_1:61708657-61708676 Msa1205410:intron 35.0%
! CACCTTGTAACTCTGAAAAA+TGG + chr8_1:61708662-61708681 None:intergenic 35.0%
!! CATTTTTCAGAGTTACAAGG+TGG - chr8_1:61708660-61708679 Msa1205410:intron 35.0%
TCAATTTGTGGCTAGAGAAA+TGG + chr8_1:61708700-61708719 None:intergenic 35.0%
ATTTCTCTAGCCACAAATTG+AGG - chr8_1:61708699-61708718 Msa1205410:intron 35.0%
TGAATCCTTGAGTATAAAGC+AGG + chr8_1:61708776-61708795 None:intergenic 35.0%
GGACAAAAACCATGCAAAAT+GGG + chr8_1:61708972-61708991 None:intergenic 35.0%
!!! CATTTTTTGTGCTCATTTGG+TGG - chr8_1:61709011-61709030 Msa1205410:intron 35.0%
CATGTTATTAGTTCGTTGAC+TGG - chr8_1:61709051-61709070 Msa1205410:intron 35.0%
TTGATTTCAAATAGCCCATC+TGG + chr8_1:61709218-61709237 None:intergenic 35.0%
! GTTTAATGAAGGTGGTTTGT+GGG - chr8_1:61709265-61709284 Msa1205410:intron 35.0%
! TGTGATTTAGAACTTGCACT+TGG - chr8_1:61709354-61709373 Msa1205410:intron 35.0%
TCATGTTCTCTTCTTTCTCA+TGG - chr8_1:61709388-61709407 Msa1205410:CDS 35.0%
AGGTTTGATTCATCACTACT+TGG - chr8_1:61709569-61709588 Msa1205410:five_prime_UTR 35.0%
TTTGATTCATCACTACTTGG+TGG - chr8_1:61709572-61709591 Msa1205410:five_prime_UTR 35.0%
! ACGAACTTCTCGGTCTATTT+TGG + chr8_1:61706106-61706125 None:intergenic 40.0%
GAGGAAGAAAAGTTTCGCTT+GGG + chr8_1:61706285-61706304 None:intergenic 40.0%
GCTGATGCGTCTCTTATATT+TGG - chr8_1:61706385-61706404 Msa1205410:three_prime_UTR 40.0%
AAATCAAATCAGAGCGAACC+AGG + chr8_1:61706481-61706500 None:intergenic 40.0%
!!! GTGCTTTGATTCGGCTTTTT+AGG - chr8_1:61706577-61706596 Msa1205410:intron 40.0%
GCATTGCTATGTATCATCGA+AGG - chr8_1:61706788-61706807 Msa1205410:intron 40.0%
AGTCTTGATATGCTTGTAGC+TGG - chr8_1:61706924-61706943 Msa1205410:intron 40.0%
GGCCGTTTCATCTTATACAA+AGG + chr8_1:61707064-61707083 None:intergenic 40.0%
! CTAGGCATTGTTTCATGTAC+CGG - chr8_1:61707105-61707124 Msa1205410:intron 40.0%
TTACAGGAGGTTCATCATAG+TGG + chr8_1:61707164-61707183 None:intergenic 40.0%
! GTTTTTACTGTCTCTGATGG+TGG + chr8_1:61707570-61707589 None:intergenic 40.0%
!!! GATATTGCTTTTTGCCGTTG+GGG - chr8_1:61707757-61707776 Msa1205410:intron 40.0%
! TGGCCTGAAAGGTGTATTAT+AGG + chr8_1:61708168-61708187 None:intergenic 40.0%
! TGCGACGTGATTCATCTTTT+AGG - chr8_1:61708195-61708214 Msa1205410:intron 40.0%
! AATTTGGTGGAATGGTTGGA+GGG + chr8_1:61708385-61708404 None:intergenic 40.0%
! AAATTTGGTGGAATGGTTGG+AGG + chr8_1:61708386-61708405 None:intergenic 40.0%
AGGCATATGTCATAGTTGGT+TGG - chr8_1:61708516-61708535 Msa1205410:intron 40.0%
! GTTGGTTGGTATAATGGAGT+TGG - chr8_1:61708530-61708549 Msa1205410:CDS 40.0%
AAATGGAGCTTGCAATATGC+CGG + chr8_1:61708645-61708664 None:intergenic 40.0%
TCCTTGAGTATAAAGCAGGA+AGG + chr8_1:61708772-61708791 None:intergenic 40.0%
! AACTTGCCGTTCATTTGTTG+AGG - chr8_1:61708800-61708819 Msa1205410:intron 40.0%
CGCATTCCTCAACAAATGAA+CGG + chr8_1:61708809-61708828 None:intergenic 40.0%
! CTTCAATCACCCATTTTGCA+TGG - chr8_1:61708960-61708979 Msa1205410:intron 40.0%
CGGACAAAAACCATGCAAAA+TGG + chr8_1:61708973-61708992 None:intergenic 40.0%
TGACAAACAATATGCACGGA+CGG + chr8_1:61708993-61709012 None:intergenic 40.0%
TGGATAACTAGAAGCAGTCA+TGG + chr8_1:61709107-61709126 None:intergenic 40.0%
CTTGTTGACCTGAAATTCCA+CGG - chr8_1:61709160-61709179 Msa1205410:intron 40.0%
AAGAGAAATGTCATACACCG+TGG + chr8_1:61709180-61709199 None:intergenic 40.0%
TGATGATAAACAGGTCCAGA+TGG - chr8_1:61709200-61709219 Msa1205410:intron 40.0%
GATGATAAACAGGTCCAGAT+GGG - chr8_1:61709201-61709220 Msa1205410:intron 40.0%
AGCACCACTAGGAATATTAG+TGG - chr8_1:61709243-61709262 Msa1205410:intron 40.0%
GGTTTAATGAAGGTGGTTTG+TGG - chr8_1:61709264-61709283 Msa1205410:intron 40.0%
AACTGCAATAACCCTGTTTG+AGG + chr8_1:61709316-61709335 None:intergenic 40.0%
ACACAGCCATTATGAAACAC+AGG + chr8_1:61709521-61709540 None:intergenic 40.0%
! TCACTACTTGGTGGATAAAG+TGG - chr8_1:61709581-61709600 Msa1205410:five_prime_UTR 40.0%
! CACTACTTGGTGGATAAAGT+GGG - chr8_1:61709582-61709601 Msa1205410:five_prime_UTR 40.0%
AGAAAAGTTTCGCTTGGGAG+CGG + chr8_1:61706280-61706299 None:intergenic 45.0%
GGAGGAAGAAAAGTTTCGCT+TGG + chr8_1:61706286-61706305 None:intergenic 45.0%
! CGAAACTTTTCTTCCTCCTC+TGG - chr8_1:61706288-61706307 Msa1205410:three_prime_UTR 45.0%
! CGTGCATTTGAAATGTAGCC+AGG + chr8_1:61707380-61707399 None:intergenic 45.0%
! GGTGTTGCATTATGAGCAGA+GGG + chr8_1:61707545-61707564 None:intergenic 45.0%
!! TGGTGTTGCATTATGAGCAG+AGG + chr8_1:61707546-61707565 None:intergenic 45.0%
!! TTACTGTCTCTGATGGTGGT+TGG + chr8_1:61707566-61707585 None:intergenic 45.0%
GAATATCCAGTGAACCCCAA+CGG + chr8_1:61707774-61707793 None:intergenic 45.0%
GAATCACGTCGCACAAACTT+TGG + chr8_1:61708188-61708207 None:intergenic 45.0%
TCCGCAATAAGGAATTCCCA+TGG - chr8_1:61708299-61708318 Msa1205410:intron 45.0%
CCCATGGGAATTCCTTATTG+CGG + chr8_1:61708303-61708322 None:intergenic 45.0%
CCGCAATAAGGAATTCCCAT+GGG - chr8_1:61708300-61708319 Msa1205410:intron 45.0%
CGCAATAAGGAATTCCCATG+GGG - chr8_1:61708301-61708320 Msa1205410:intron 45.0%
GTGAATGACACATACCCCAT+GGG + chr8_1:61708318-61708337 None:intergenic 45.0%
GCCTTCCTGCTTTATACTCA+AGG - chr8_1:61708768-61708787 Msa1205410:intron 45.0%
GTCATACACCGTGGAATTTC+AGG + chr8_1:61709171-61709190 None:intergenic 45.0%
AATCAAGAACGAGCACCACT+AGG - chr8_1:61709232-61709251 Msa1205410:intron 45.0%
TGGGCACTAATGTCTTCCTT+TGG - chr8_1:61709284-61709303 Msa1205410:intron 45.0%
TTTGGCTGTAGCCTCAAACA+GGG - chr8_1:61709302-61709321 Msa1205410:intron 45.0%
!!! TGAATTATATTTTAATAATT+AGG - chr8_1:61706651-61706670 Msa1205410:intron 5.0%
GAAAAGTTTCGCTTGGGAGC+GGG + chr8_1:61706279-61706298 None:intergenic 50.0%
ATCATCGTGATGACCAGAGG+AGG + chr8_1:61706304-61706323 None:intergenic 50.0%
TGCATCATCGTGATGACCAG+AGG + chr8_1:61706307-61706326 None:intergenic 50.0%
GAGGTTCATCATAGTGGTGG+TGG + chr8_1:61707158-61707177 None:intergenic 50.0%
CAGGAGGTTCATCATAGTGG+TGG + chr8_1:61707161-61707180 None:intergenic 50.0%
ATGTACTCTTCTCCAGCCCA+AGG - chr8_1:61707648-61707667 Msa1205410:intron 50.0%
! AGTCATTTGTTGCCTTGGGC+TGG + chr8_1:61707663-61707682 None:intergenic 50.0%
! TTTTTGCCGTTGGGGTTCAC+TGG - chr8_1:61707765-61707784 Msa1205410:intron 50.0%
CGCACAAACTTTGGCCTGAA+AGG + chr8_1:61708179-61708198 None:intergenic 50.0%
CGTGAATGACACATACCCCA+TGG + chr8_1:61708319-61708338 None:intergenic 50.0%
!! GAATGGTTGGAGGGCACTTT+AGG + chr8_1:61708376-61708395 None:intergenic 50.0%
! CTGTTTGAGGCTACAGCCAA+AGG + chr8_1:61709303-61709322 None:intergenic 50.0%
CTTTGGCTGTAGCCTCAAAC+AGG - chr8_1:61709301-61709320 Msa1205410:intron 50.0%
GCGACGACGTTGATGCGATC+TGG - chr8_1:61706210-61706229 Msa1205410:three_prime_UTR 60.0%
CTTCGCAACGTCCTCCGACG+AGG - chr8_1:61706237-61706256 Msa1205410:three_prime_UTR 65.0%
GTTTCGCTTGGGAGCGGGAG+AGG + chr8_1:61706274-61706293 None:intergenic 65.0%
CGCAACGTCCTCCGACGAGG+AGG - chr8_1:61706240-61706259 Msa1205410:three_prime_UTR 70.0%
GTCCTCCGACGAGGAGGCTC+CGG - chr8_1:61706246-61706265 Msa1205410:three_prime_UTR 70.0%
!! AGGCACCGGAGCCTCCTCGT+CGG + chr8_1:61706254-61706273 None:intergenic 70.0%
! CTTGGGAGCGGGAGAGGCAC+CGG + chr8_1:61706268-61706287 None:intergenic 70.0%
CACCGGAGCCTCCTCGTCGG+AGG + chr8_1:61706251-61706270 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 61706103 61709605 61706103 ID=Msa1205410;Name=Msa1205410
chr8_1 mRNA 61706103 61709605 61706103 ID=Msa1205410-mRNA-1;Parent=Msa1205410;Name=Msa1205410-mRNA-1;_AED=0.01;_eAED=0.01;_QI=104|1|1|1|1|1|4|352|103
chr8_1 exon 61706103 61706495 61706103 ID=Msa1205410-mRNA-1:exon:20019;Parent=Msa1205410-mRNA-1
chr8_1 exon 61707386 61707428 61707386 ID=Msa1205410-mRNA-1:exon:20018;Parent=Msa1205410-mRNA-1
chr8_1 exon 61708529 61708635 61708529 ID=Msa1205410-mRNA-1:exon:20017;Parent=Msa1205410-mRNA-1
chr8_1 exon 61709381 61709605 61709381 ID=Msa1205410-mRNA-1:exon:20016;Parent=Msa1205410-mRNA-1
chr8_1 five_prime_UTR 61709502 61709605 61709502 ID=Msa1205410-mRNA-1:five_prime_utr;Parent=Msa1205410-mRNA-1
chr8_1 CDS 61709381 61709501 61709381 ID=Msa1205410-mRNA-1:cds;Parent=Msa1205410-mRNA-1
chr8_1 CDS 61708529 61708635 61708529 ID=Msa1205410-mRNA-1:cds;Parent=Msa1205410-mRNA-1
chr8_1 CDS 61707386 61707428 61707386 ID=Msa1205410-mRNA-1:cds;Parent=Msa1205410-mRNA-1
chr8_1 CDS 61706455 61706495 61706455 ID=Msa1205410-mRNA-1:cds;Parent=Msa1205410-mRNA-1
chr8_1 three_prime_UTR 61706103 61706454 61706103 ID=Msa1205410-mRNA-1:three_prime_utr;Parent=Msa1205410-mRNA-1
Gene Sequence

>Msa1205410

ATGGCGACGACGTTGATGCGATCTGGTTTTCTTCGCAACGTCCTCCGACGAGGAGGCTCCGGTGCCTCTCCCGCTCCCAAGCGAAACTTTTCTTCCTCCTCTGGTCATCACGATGATGCATATGAAACGGCCAAATGGGAGAAGATTACATATCTAGGCATTGTTTCATGTACCGGTTTAGCTGTTTATACTCTATCAAAGGGCCACCACCACTATGATGAACCTCCTGCATACCCATATTTGCATATCCGCAATAAGGAATTCCCATGGGGTCCAGATGGGCTATTTGAAATCAAGAACGAGCACCACTAG
Protein sequence

>Msa1205410

MATTLMRSGFLRNVLRRGGSGASPAPKRNFSSSSGHHDDAYETAKWEKITYLGIVSCTGLAVYTLSKGHHHYDEPPAYPYLHIRNKEFPWGPDGLFEIKNEHH*