AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1297430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1297430 MtrunA17_Chr4g0054491 97.087 103 3 0 1 103 1 103 3.34e-73 212
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1297430 AT4G37830.1 71.154 104 27 3 1 103 1 102 1.36e-48 150
Msa1297430 AT4G37830.2 63.462 104 25 4 1 103 1 92 2.32e-38 124
Msa1297430 AT4G37830.3 67.532 77 23 2 1 77 1 75 3.14e-29 103

Find 60 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGAACTTCTCGGTCTATTT+TGG 0.117274 8_3:-57715990 None:intergenic
TTATTGCGGATATGCAAATA+TGG 0.260738 8_3:-57718083 None:intergenic
CTTTGGCTGTAGCCTCAAAC+AGG 0.332714 8_3:+57719098 Msa1297430:three_prime_UTR
CAAACATCATATTGTTTGTT+GGG 0.352046 8_3:-57719227 None:intergenic
TCATGTTCTCTTCTTTCTCA+TGG 0.360031 8_3:+57719185 Msa1297430:three_prime_UTR
TTGATTTCAAATAGCCCATC+TGG 0.366613 8_3:-57719012 None:intergenic
ACAAACATCATATTGTTTGT+TGG 0.382436 8_3:-57719228 None:intergenic
ATATTTGCATATCCGCAATA+AGG 0.383345 8_3:+57718085 Msa1297430:CDS
ATATGTAATCTTCTCCCATT+TGG 0.387052 8_3:-57716938 None:intergenic
TGGGCACTAATGTCTTCCTT+TGG 0.396894 8_3:+57719081 Msa1297430:three_prime_UTR
TGGGAGAAGATTACATATCT+AGG 0.412735 8_3:+57716943 Msa1297430:CDS
TATTTCGAAGAAGAGTAAAA+TGG 0.414452 8_3:+57716075 Msa1297430:exon
CTAGGCATTGTTTCATGTAC+CGG 0.435132 8_3:+57716961 Msa1297430:CDS
AGAAAAGTTTCGCTTGGGAG+CGG 0.441949 8_3:-57716164 None:intergenic
TATAAGATGAAACGGCCAAA+TGG 0.446760 8_3:+57716923 Msa1297430:intron
GGAGGAAGAAAAGTTTCGCT+TGG 0.451508 8_3:-57716170 None:intergenic
GCGACGACGTTGATGCGATC+TGG 0.452055 8_3:+57716097 Msa1297430:CDS
CTCCTTTGTATAAGATGAAA+CGG 0.453752 8_3:+57716915 Msa1297430:intron
GTTTCGCTTGGGAGCGGGAG+AGG 0.459261 8_3:-57716158 None:intergenic
GTCCTCCGACGAGGAGGCTC+CGG 0.462096 8_3:+57716133 Msa1297430:CDS
GAGGAAGAAAAGTTTCGCTT+GGG 0.467177 8_3:-57716169 None:intergenic
GAAAAGTTTCGCTTGGGAGC+GGG 0.478655 8_3:-57716163 None:intergenic
TCACTACTTGGTGGATAAAG+TGG 0.483260 8_3:+57719378 Msa1297430:three_prime_UTR
TAGAGTATAAACAGCTAAAC+CGG 0.486251 8_3:-57716980 None:intergenic
AGCACCACTAGGAATATTAG+TGG 0.487416 8_3:+57719040 Msa1297430:exon
AGCTGTTTATACTCTATCAA+AGG 0.488901 8_3:+57716987 Msa1297430:CDS
CTTGGGAGCGGGAGAGGCAC+CGG 0.489102 8_3:-57716152 None:intergenic
GGTTTAATGAAGGTGGTTTG+TGG 0.489309 8_3:+57719061 Msa1297430:three_prime_UTR
TTTGATTCATCACTACTTGG+TGG 0.490515 8_3:+57719369 Msa1297430:three_prime_UTR
GCTGTTTATACTCTATCAAA+GGG 0.495796 8_3:+57716988 Msa1297430:CDS
AGGTTTGATTCATCACTACT+TGG 0.501061 8_3:+57719366 Msa1297430:three_prime_UTR
GAATATTAGTGGTTTAATGA+AGG 0.504937 8_3:+57719051 Msa1297430:three_prime_UTR
AACTGCAATAACCCTGTTTG+AGG 0.513422 8_3:-57719110 None:intergenic
ATAAGATGAAACGGCCAAAT+GGG 0.513873 8_3:+57716924 Msa1297430:intron
TATTAGTGGTTTAATGAAGG+TGG 0.519985 8_3:+57719054 Msa1297430:three_prime_UTR
GTTTAATGAAGGTGGTTTGT+GGG 0.527505 8_3:+57719062 Msa1297430:three_prime_UTR
TATTGCGGATATGCAAATAT+GGG 0.531200 8_3:-57718082 None:intergenic
TGATGATAAACAGGTCCAGA+TGG 0.532448 8_3:+57718997 Msa1297430:intron
GAGGTTCATCATAGTGGTGG+TGG 0.540593 8_3:-57717011 None:intergenic
CTGTTTGAGGCTACAGCCAA+AGG 0.572052 8_3:-57719097 None:intergenic
CTTCGCAACGTCCTCCGACG+AGG 0.587981 8_3:+57716124 Msa1297430:CDS
TGTGATTTAGAACTTGCACT+TGG 0.591187 8_3:+57719151 Msa1297430:three_prime_UTR
CACTACTTGGTGGATAAAGT+GGG 0.593788 8_3:+57719379 Msa1297430:three_prime_UTR
CACCGGAGCCTCCTCGTCGG+AGG 0.600406 8_3:-57716135 None:intergenic
GATGATAAACAGGTCCAGAT+GGG 0.601053 8_3:+57718998 Msa1297430:intron
CCCATGGGAATTCCTTATTG+CGG 0.601121 8_3:-57718097 None:intergenic
TTACAGGAGGTTCATCATAG+TGG 0.602947 8_3:-57717017 None:intergenic
AATCAAGAACGAGCACCACT+AGG 0.603430 8_3:+57719029 Msa1297430:CDS
TCCGCAATAAGGAATTCCCA+TGG 0.603749 8_3:+57718096 Msa1297430:CDS
CCGCAATAAGGAATTCCCAT+GGG 0.606158 8_3:+57718097 Msa1297430:CDS
CGCAACGTCCTCCGACGAGG+AGG 0.607912 8_3:+57716127 Msa1297430:CDS
ACACAGCCATTATGAAACAC+AGG 0.639520 8_3:-57719315 None:intergenic
TGCATCATCGTGATGACCAG+AGG 0.640046 8_3:-57716191 None:intergenic
TTTGGCTGTAGCCTCAAACA+GGG 0.644011 8_3:+57719099 Msa1297430:three_prime_UTR
CAGGAGGTTCATCATAGTGG+TGG 0.646029 8_3:-57717014 None:intergenic
GTGAATGACACATACCCCAT+GGG 0.647797 8_3:-57718112 None:intergenic
AGGCACCGGAGCCTCCTCGT+CGG 0.660173 8_3:-57716138 None:intergenic
ATCATCGTGATGACCAGAGG+AGG 0.665684 8_3:-57716188 None:intergenic
TAAACCACTAATATTCCTAG+TGG 0.676016 8_3:-57719044 None:intergenic
CGCAATAAGGAATTCCCATG+GGG 0.679540 8_3:+57718098 Msa1297430:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTAAAACTATAAGTAA+TGG + chr8_3:57717245-57717264 Msa1297430:intron 10.0%
!!! TCAATTTTTCAATATGAATA+TGG + chr8_3:57716315-57716334 Msa1297430:intron 15.0%
!!! CAATTTTTCAATATGAATAT+GGG + chr8_3:57716316-57716335 Msa1297430:intron 15.0%
!! TTATTCATTCTTGAAATAAT+GGG - chr8_3:57717804-57717823 None:intergenic 15.0%
!!! TTTTCTGTGTTTTTTACAAA+TGG - chr8_3:57716046-57716065 None:intergenic 20.0%
!! TATACAAAATTCAACATGAA+AGG - chr8_3:57717079-57717098 None:intergenic 20.0%
!!! TCATTTTATTAGAAAAGAAC+AGG - chr8_3:57717224-57717243 None:intergenic 20.0%
!! TGGAAGTTTATAAATAAAAG+AGG + chr8_3:57717265-57717284 Msa1297430:intron 20.0%
!!! TTGATCTTTTATTCAGTTAA+AGG - chr8_3:57717306-57717325 None:intergenic 20.0%
!! ATAAATAGAATAAACACTGT+TGG + chr8_3:57717481-57717500 Msa1297430:intron 20.0%
!! GAACTATTAAAATTCACATA+TGG - chr8_3:57717617-57717636 None:intergenic 20.0%
!! CTTATTCATTCTTGAAATAA+TGG - chr8_3:57717805-57717824 None:intergenic 20.0%
!! TTTCAAGAATGAATAAGAAA+AGG + chr8_3:57717808-57717827 Msa1297430:intron 20.0%
!!! AATCTACTGTATTTATTTTC+CGG - chr8_3:57717889-57717908 None:intergenic 20.0%
!! ACAAGATAATTTGAAAACAA+AGG - chr8_3:57718942-57718961 None:intergenic 20.0%
!!! TTTTATTTCAAGTCTTGTTT+AGG + chr8_3:57719251-57719270 Msa1297430:three_prime_UTR 20.0%
!! AAGTTACCAAAAATATAACT+AGG + chr8_3:57719346-57719365 Msa1297430:three_prime_UTR 20.0%
!!! ATCAAACCTAGTTATATTTT+TGG - chr8_3:57719355-57719374 None:intergenic 20.0%
! TATTTCGAAGAAGAGTAAAA+TGG + chr8_3:57716075-57716094 Msa1297430:exon 25.0%
! AACCCTAAATGAATGTAAAA+GGG - chr8_3:57716226-57716245 None:intergenic 25.0%
! AAACCCTAAATGAATGTAAA+AGG - chr8_3:57716227-57716246 None:intergenic 25.0%
!! CTCATATATCAGGTTAATTT+TGG + chr8_3:57716282-57716301 Msa1297430:intron 25.0%
! TTCAATATGAATATGGGAAA+TGG + chr8_3:57716322-57716341 Msa1297430:intron 25.0%
! AAATGACCAAATCGAATTTA+TGG + chr8_3:57716532-57716551 Msa1297430:intron 25.0%
! AATGACCAAATCGAATTTAT+GGG + chr8_3:57716533-57716552 Msa1297430:intron 25.0%
!!! AGTGGATTCAATAAGATTTT+GGG - chr8_3:57716577-57716596 None:intergenic 25.0%
!!! TAGTGGATTCAATAAGATTT+TGG - chr8_3:57716578-57716597 None:intergenic 25.0%
! AAAATCAATTCTTGATATGC+TGG + chr8_3:57716773-57716792 Msa1297430:intron 25.0%
! AGCATGTAAAGAAGAATTTA+AGG - chr8_3:57716863-57716882 None:intergenic 25.0%
!! GATATGTATTCATTTTCTCT+TGG + chr8_3:57717156-57717175 Msa1297430:intron 25.0%
!! TTTTATTCCTTTTCATTTCC+AGG + chr8_3:57717510-57717529 Msa1297430:intron 25.0%
!! TTTATTCCTTTTCATTTCCA+GGG + chr8_3:57717511-57717530 Msa1297430:intron 25.0%
! CATGAATGACTTAACTTTAT+GGG + chr8_3:57717723-57717742 Msa1297430:intron 25.0%
! AGAATGAATAAGAAAAGGAA+TGG + chr8_3:57717813-57717832 Msa1297430:intron 25.0%
! CATAGACAAATATGAATGTT+CGG - chr8_3:57717863-57717882 None:intergenic 25.0%
!!! ATCTACTGTATTTATTTTCC+GGG - chr8_3:57717888-57717907 None:intergenic 25.0%
! TCTAATCAATCCAACAAATT+TGG - chr8_3:57718198-57718217 None:intergenic 25.0%
! AGAAATAAGTAATATTGCAG+TGG - chr8_3:57718356-57718375 None:intergenic 25.0%
! TAGTCTAGTTGCAATTAATT+GGG + chr8_3:57718392-57718411 Msa1297430:intron 25.0%
! AATAACAATTCCTCAATTTG+TGG - chr8_3:57718509-57718528 None:intergenic 25.0%
!!! CTCTTTTTTGTTTACATGTT+AGG + chr8_3:57718543-57718562 Msa1297430:intron 25.0%
!!! ATTTATCCATAAAGTACTGT+TGG - chr8_3:57718648-57718667 None:intergenic 25.0%
!!! AATTTTGATAGACAAGTCTT+TGG + chr8_3:57718665-57718684 Msa1297430:intron 25.0%
! GAAAATCGTTAATTATGTTG+AGG - chr8_3:57718698-57718717 None:intergenic 25.0%
! AAAATGACAAACAATATGCA+CGG - chr8_3:57718794-57718813 None:intergenic 25.0%
!! ATTTCTCTTTGATGATAAAC+AGG + chr8_3:57718988-57719007 Msa1297430:intron 25.0%
! GAATATTAGTGGTTTAATGA+AGG + chr8_3:57719051-57719070 Msa1297430:three_prime_UTR 25.0%
! CAAACATCATATTGTTTGTT+GGG - chr8_3:57719230-57719249 None:intergenic 25.0%
! ACAAACATCATATTGTTTGT+TGG - chr8_3:57719231-57719250 None:intergenic 25.0%
AGAAGAAAAAACGAACTTCT+CGG - chr8_3:57716003-57716022 None:intergenic 30.0%
! TACCCCTTTTACATTCATTT+AGG + chr8_3:57716220-57716239 Msa1297430:intron 30.0%
! ACCCCTTTTACATTCATTTA+GGG + chr8_3:57716221-57716240 Msa1297430:intron 30.0%
ACCCTAAATGAATGTAAAAG+GGG - chr8_3:57716225-57716244 None:intergenic 30.0%
! GTTGATTCTTCTCATATATC+AGG + chr8_3:57716272-57716291 Msa1297430:intron 30.0%
AATGAATTTGTGCTCAGAAA+TGG + chr8_3:57716455-57716474 Msa1297430:intron 30.0%
CAATTCAAATGCAAACTTAG+TGG - chr8_3:57716595-57716614 None:intergenic 30.0%
TAAGTTTGCATTTGAATTGC+TGG + chr8_3:57716596-57716615 Msa1297430:intron 30.0%
CAATGCAAATACAAATCCAT+TGG - chr8_3:57716630-57716649 None:intergenic 30.0%
! TGTATCATCGAAGGTTATTT+TGG + chr8_3:57716653-57716672 Msa1297430:intron 30.0%
!! GTATCATCGAAGGTTATTTT+GGG + chr8_3:57716654-57716673 Msa1297430:intron 30.0%
!! TATCATCGAAGGTTATTTTG+GGG + chr8_3:57716655-57716674 Msa1297430:intron 30.0%
!! ATAGATCTTGTAGCTGTTTT+AGG + chr8_3:57716806-57716825 Msa1297430:intron 30.0%
CTCCTTTGTATAAGATGAAA+CGG + chr8_3:57716915-57716934 Msa1297430:intron 30.0%
ATATGTAATCTTCTCCCATT+TGG - chr8_3:57716941-57716960 None:intergenic 30.0%
TAGAGTATAAACAGCTAAAC+CGG - chr8_3:57716983-57717002 None:intergenic 30.0%
AGCTGTTTATACTCTATCAA+AGG + chr8_3:57716987-57717006 Msa1297430:CDS 30.0%
GCTGTTTATACTCTATCAAA+GGG + chr8_3:57716988-57717007 Msa1297430:CDS 30.0%
TATAACAGAAAACTTACAGG+AGG - chr8_3:57717033-57717052 None:intergenic 30.0%
CCATATAACAGAAAACTTAC+AGG - chr8_3:57717036-57717055 None:intergenic 30.0%
! CCTGTAAGTTTTCTGTTATA+TGG + chr8_3:57717033-57717052 Msa1297430:intron 30.0%
!!! ATTGTTTTTACCGTCTTTGA+TGG - chr8_3:57717370-57717389 None:intergenic 30.0%
! TAGTTTCTTTGACTTCGAAA+AGG + chr8_3:57717398-57717417 Msa1297430:intron 30.0%
TTTGTTACAGTCTATATAGC+TGG - chr8_3:57717670-57717689 None:intergenic 30.0%
GCATGAATGACTTAACTTTA+TGG + chr8_3:57717722-57717741 Msa1297430:intron 30.0%
! CTTAACTTTATGGGAAGTTT+TGG + chr8_3:57717732-57717751 Msa1297430:intron 30.0%
!!! TTATGGGAAGTTTTGGAATT+TGG + chr8_3:57717739-57717758 Msa1297430:intron 30.0%
AAAAGGAATGGCTTAAAATG+AGG + chr8_3:57717825-57717844 Msa1297430:intron 30.0%
TTCATATTTGTCTATGTACC+CGG + chr8_3:57717867-57717886 Msa1297430:intron 30.0%
GAAACAAATGACTACACATA+AGG - chr8_3:57717943-57717962 None:intergenic 30.0%
TTTCCTATAATACACCTTTC+AGG + chr8_3:57717959-57717978 Msa1297430:intron 30.0%
AACTCTTCTATTTATGATGC+AGG + chr8_3:57718055-57718074 Msa1297430:intron 30.0%
TATTGCGGATATGCAAATAT+GGG - chr8_3:57718085-57718104 None:intergenic 30.0%
TTATTGCGGATATGCAAATA+TGG - chr8_3:57718086-57718105 None:intergenic 30.0%
ATATTTGCATATCCGCAATA+AGG + chr8_3:57718085-57718104 Msa1297430:CDS 30.0%
AATCAATCCAACAAATTTGG+TGG - chr8_3:57718195-57718214 None:intergenic 30.0%
! AATTTAGAATGCAGCAATGA+AGG - chr8_3:57718232-57718251 None:intergenic 30.0%
! ACATTTGTTAAGCACAAGAA+TGG + chr8_3:57718292-57718311 Msa1297430:intron 30.0%
! CATTTGTTAAGCACAAGAAT+GGG + chr8_3:57718293-57718312 Msa1297430:intron 30.0%
! ATCATAGTTGGTTGGTATAA+TGG + chr8_3:57718321-57718340 Msa1297430:intron 30.0%
CTAGTCTAGTTGCAATTAAT+TGG + chr8_3:57718391-57718410 Msa1297430:intron 30.0%
TTGGGATAAGCTTCATATTA+TGG + chr8_3:57718410-57718429 Msa1297430:intron 30.0%
CTTCATATTATGGTATCTAC+CGG + chr8_3:57718420-57718439 Msa1297430:intron 30.0%
CAAATACCAACAGTACTTTA+TGG + chr8_3:57718639-57718658 Msa1297430:intron 30.0%
! AAAATGGGTGATTGAAGTTT+TGG - chr8_3:57718754-57718773 None:intergenic 30.0%
!!! TGTCATTTTTTGTGCTCATT+TGG + chr8_3:57718805-57718824 Msa1297430:intron 30.0%
! AAAGGCATACACAATATCAA+TGG - chr8_3:57718924-57718943 None:intergenic 30.0%
TAAACCACTAATATTCCTAG+TGG - chr8_3:57719047-57719066 None:intergenic 30.0%
TATTAGTGGTTTAATGAAGG+TGG + chr8_3:57719054-57719073 Msa1297430:three_prime_UTR 30.0%
! CAGGTTTTTCTTGTCATATT+CGG - chr8_3:57719299-57719318 None:intergenic 30.0%
GAAAAACCTGTGTTTCATAA+TGG + chr8_3:57719309-57719328 Msa1297430:three_prime_UTR 30.0%
TGAATATGGGAAATGGTATG+TGG + chr8_3:57716329-57716348 Msa1297430:intron 35.0%
!! TGTGGATTTTCATTTTGACC+TGG + chr8_3:57716347-57716366 Msa1297430:intron 35.0%
ATTAGGCAAAATCACTCTCA+TGG + chr8_3:57716495-57716514 Msa1297430:intron 35.0%
CTTGTCCCATAAATTCGATT+TGG - chr8_3:57716541-57716560 None:intergenic 35.0%
! ATTGCTGGATTTTACTCCAA+TGG + chr8_3:57716611-57716630 Msa1297430:intron 35.0%
TATAAGATGAAACGGCCAAA+TGG + chr8_3:57716923-57716942 Msa1297430:intron 35.0%
ATAAGATGAAACGGCCAAAT+GGG + chr8_3:57716924-57716943 Msa1297430:intron 35.0%
TGGGAGAAGATTACATATCT+AGG + chr8_3:57716943-57716962 Msa1297430:CDS 35.0%
! AGAGTACGTCAATATTTCTG+CGG - chr8_3:57717434-57717453 None:intergenic 35.0%
! TATGAGTCATTTGTTGCCTT+GGG - chr8_3:57717464-57717483 None:intergenic 35.0%
! TTATGAGTCATTTGTTGCCT+TGG - chr8_3:57717465-57717484 None:intergenic 35.0%
AAAAAGCCCTGGAAATGAAA+AGG - chr8_3:57717520-57717539 None:intergenic 35.0%
TGATTATGACTAAAAAGCCC+TGG - chr8_3:57717531-57717550 None:intergenic 35.0%
!!! AGATATTGCTTTTTGCCGTT+GGG + chr8_3:57717553-57717572 Msa1297430:intron 35.0%
!! GCAAAGCATTGATGCTTTAT+AGG - chr8_3:57717648-57717667 None:intergenic 35.0%
TCATAGAATAACTTCTGAGC+TGG - chr8_3:57717912-57717931 None:intergenic 35.0%
! AACAAATTTGGTGGAATGGT+TGG - chr8_3:57718186-57718205 None:intergenic 35.0%
AACCATTCCACCAAATTTGT+TGG + chr8_3:57718185-57718204 Msa1297430:intron 35.0%
! ATCCAACAAATTTGGTGGAA+TGG - chr8_3:57718190-57718209 None:intergenic 35.0%
GAATGGGCATATATCATAGT+TGG + chr8_3:57718309-57718328 Msa1297430:intron 35.0%
!! CTCCATTTTTCAGAGTTACA+AGG + chr8_3:57718454-57718473 Msa1297430:intron 35.0%
! CACCTTGTAACTCTGAAAAA+TGG - chr8_3:57718459-57718478 None:intergenic 35.0%
!! CATTTTTCAGAGTTACAAGG+TGG + chr8_3:57718457-57718476 Msa1297430:intron 35.0%
TCAATTTGTGGCTAGAGAAA+TGG - chr8_3:57718497-57718516 None:intergenic 35.0%
ATTTCTCTAGCCACAAATTG+AGG + chr8_3:57718496-57718515 Msa1297430:intron 35.0%
TGAATCCTTGAGTATAAAGC+AGG - chr8_3:57718573-57718592 None:intergenic 35.0%
! AACTTGACGTTCATTTGTTG+AGG + chr8_3:57718597-57718616 Msa1297430:intron 35.0%
GGACAAAAACCATGCAAAAT+GGG - chr8_3:57718769-57718788 None:intergenic 35.0%
!!! CATTTTTTGTGCTCATTTGG+TGG + chr8_3:57718808-57718827 Msa1297430:intron 35.0%
CATGTTATTAGTTCGTTGAC+TGG + chr8_3:57718848-57718867 Msa1297430:intron 35.0%
TTGATTTCAAATAGCCCATC+TGG - chr8_3:57719015-57719034 None:intergenic 35.0%
! GTTTAATGAAGGTGGTTTGT+GGG + chr8_3:57719062-57719081 Msa1297430:three_prime_UTR 35.0%
! TGTGATTTAGAACTTGCACT+TGG + chr8_3:57719151-57719170 Msa1297430:three_prime_UTR 35.0%
TCATGTTCTCTTCTTTCTCA+TGG + chr8_3:57719185-57719204 Msa1297430:three_prime_UTR 35.0%
AGGTTTGATTCATCACTACT+TGG + chr8_3:57719366-57719385 Msa1297430:three_prime_UTR 35.0%
TTTGATTCATCACTACTTGG+TGG + chr8_3:57719369-57719388 Msa1297430:three_prime_UTR 35.0%
! ACGAACTTCTCGGTCTATTT+TGG - chr8_3:57715993-57716012 None:intergenic 40.0%
GAGGAAGAAAAGTTTCGCTT+GGG - chr8_3:57716172-57716191 None:intergenic 40.0%
AAATCAAATCAGAGCGAACC+AGG - chr8_3:57716368-57716387 None:intergenic 40.0%
GCATTGCTATGTATCATCGA+AGG + chr8_3:57716644-57716663 Msa1297430:intron 40.0%
ATTCTTGATATGCTGGTAGC+TGG + chr8_3:57716780-57716799 Msa1297430:intron 40.0%
GGCCGTTTCATCTTATACAA+AGG - chr8_3:57716920-57716939 None:intergenic 40.0%
! CTAGGCATTGTTTCATGTAC+CGG + chr8_3:57716961-57716980 Msa1297430:CDS 40.0%
TTACAGGAGGTTCATCATAG+TGG - chr8_3:57717020-57717039 None:intergenic 40.0%
! GTTTTTACCGTCTTTGATGG+TGG - chr8_3:57717367-57717386 None:intergenic 40.0%
!!! CAGATATTGCTTTTTGCCGT+TGG + chr8_3:57717552-57717571 Msa1297430:intron 40.0%
!!! GATATTGCTTTTTGCCGTTG+GGG + chr8_3:57717554-57717573 Msa1297430:intron 40.0%
! TGGCCTGAAAGGTGTATTAT+AGG - chr8_3:57717965-57717984 None:intergenic 40.0%
! AATTTGGTGGAATGGTTGGA+GGG - chr8_3:57718182-57718201 None:intergenic 40.0%
! AAATTTGGTGGAATGGTTGG+AGG - chr8_3:57718183-57718202 None:intergenic 40.0%
GGGCATATATCATAGTTGGT+TGG + chr8_3:57718313-57718332 Msa1297430:intron 40.0%
! GTTGGTTGGTATAATGGAGT+TGG + chr8_3:57718327-57718346 Msa1297430:intron 40.0%
AAATGGAGCTTGCAATATGC+CGG - chr8_3:57718442-57718461 None:intergenic 40.0%
TCCTTGAGTATAAAGCAGGA+AGG - chr8_3:57718569-57718588 None:intergenic 40.0%
! CTTCAATCACCCATTTTGCA+TGG + chr8_3:57718757-57718776 Msa1297430:intron 40.0%
CGGACAAAAACCATGCAAAA+TGG - chr8_3:57718770-57718789 None:intergenic 40.0%
TGACAAACAATATGCACGGA+CGG - chr8_3:57718790-57718809 None:intergenic 40.0%
TGGATAACTAGAAGCAGTCA+TGG - chr8_3:57718904-57718923 None:intergenic 40.0%
CTTGTTGACCTGAAATTCCA+CGG + chr8_3:57718957-57718976 Msa1297430:intron 40.0%
AAGAGAAATGTCATACACCG+TGG - chr8_3:57718977-57718996 None:intergenic 40.0%
TGATGATAAACAGGTCCAGA+TGG + chr8_3:57718997-57719016 Msa1297430:intron 40.0%
GATGATAAACAGGTCCAGAT+GGG + chr8_3:57718998-57719017 Msa1297430:intron 40.0%
AGCACCACTAGGAATATTAG+TGG + chr8_3:57719040-57719059 Msa1297430:exon 40.0%
GGTTTAATGAAGGTGGTTTG+TGG + chr8_3:57719061-57719080 Msa1297430:three_prime_UTR 40.0%
AACTGCAATAACCCTGTTTG+AGG - chr8_3:57719113-57719132 None:intergenic 40.0%
ACACAGCCATTATGAAACAC+AGG - chr8_3:57719318-57719337 None:intergenic 40.0%
! TCACTACTTGGTGGATAAAG+TGG + chr8_3:57719378-57719397 Msa1297430:three_prime_UTR 40.0%
! CACTACTTGGTGGATAAAGT+GGG + chr8_3:57719379-57719398 Msa1297430:three_prime_UTR 40.0%
AGAAAAGTTTCGCTTGGGAG+CGG - chr8_3:57716167-57716186 None:intergenic 45.0%
GGAGGAAGAAAAGTTTCGCT+TGG - chr8_3:57716173-57716192 None:intergenic 45.0%
! CGAAACTTTTCTTCCTCCTC+TGG + chr8_3:57716175-57716194 Msa1297430:CDS 45.0%
! GGTGTTGCATTATGAGCAGA+GGG - chr8_3:57717342-57717361 None:intergenic 45.0%
!! TGGTGTTGCATTATGAGCAG+AGG - chr8_3:57717343-57717362 None:intergenic 45.0%
ACACCAACCACCATCAAAGA+CGG + chr8_3:57717357-57717376 Msa1297430:intron 45.0%
!! TTACCGTCTTTGATGGTGGT+TGG - chr8_3:57717363-57717382 None:intergenic 45.0%
GAATATCCAGTGAACCCCAA+CGG - chr8_3:57717571-57717590 None:intergenic 45.0%
CTTTCAGGCCAAAGTTTGTG+CGG + chr8_3:57717974-57717993 Msa1297430:intron 45.0%
! TGCGGCGTGATTCATCTTTT+AGG + chr8_3:57717992-57718011 Msa1297430:intron 45.0%
TCCGCAATAAGGAATTCCCA+TGG + chr8_3:57718096-57718115 Msa1297430:CDS 45.0%
CCCATGGGAATTCCTTATTG+CGG - chr8_3:57718100-57718119 None:intergenic 45.0%
CCGCAATAAGGAATTCCCAT+GGG + chr8_3:57718097-57718116 Msa1297430:CDS 45.0%
CGCAATAAGGAATTCCCATG+GGG + chr8_3:57718098-57718117 Msa1297430:CDS 45.0%
GTGAATGACACATACCCCAT+GGG - chr8_3:57718115-57718134 None:intergenic 45.0%
GCCTTCCTGCTTTATACTCA+AGG + chr8_3:57718565-57718584 Msa1297430:intron 45.0%
GTCATACACCGTGGAATTTC+AGG - chr8_3:57718968-57718987 None:intergenic 45.0%
AATCAAGAACGAGCACCACT+AGG + chr8_3:57719029-57719048 Msa1297430:CDS 45.0%
TGGGCACTAATGTCTTCCTT+TGG + chr8_3:57719081-57719100 Msa1297430:three_prime_UTR 45.0%
TTTGGCTGTAGCCTCAAACA+GGG + chr8_3:57719099-57719118 Msa1297430:three_prime_UTR 45.0%
!!! TGAATTATATTTTAATAATT+AGG + chr8_3:57716478-57716497 Msa1297430:intron 5.0%
GAAAAGTTTCGCTTGGGAGC+GGG - chr8_3:57716166-57716185 None:intergenic 50.0%
ATCATCGTGATGACCAGAGG+AGG - chr8_3:57716191-57716210 None:intergenic 50.0%
TGCATCATCGTGATGACCAG+AGG - chr8_3:57716194-57716213 None:intergenic 50.0%
GAGGTTCATCATAGTGGTGG+TGG - chr8_3:57717014-57717033 None:intergenic 50.0%
CAGGAGGTTCATCATAGTGG+TGG - chr8_3:57717017-57717036 None:intergenic 50.0%
! AGTCATTTGTTGCCTTGGGC+TGG - chr8_3:57717460-57717479 None:intergenic 50.0%
! TTTTTGCCGTTGGGGTTCAC+TGG + chr8_3:57717562-57717581 Msa1297430:intron 50.0%
CGCACAAACTTTGGCCTGAA+AGG - chr8_3:57717976-57717995 None:intergenic 50.0%
GAATCACGCCGCACAAACTT+TGG - chr8_3:57717985-57718004 None:intergenic 50.0%
CGTGAATGACACATACCCCA+TGG - chr8_3:57718116-57718135 None:intergenic 50.0%
!! GAATGGTTGGAGGGCACTTT+AGG - chr8_3:57718173-57718192 None:intergenic 50.0%
! CTGTTTGAGGCTACAGCCAA+AGG - chr8_3:57719100-57719119 None:intergenic 50.0%
CTTTGGCTGTAGCCTCAAAC+AGG + chr8_3:57719098-57719117 Msa1297430:three_prime_UTR 50.0%
ACGTACTCTTCTCCAGCCCA+AGG + chr8_3:57717445-57717464 Msa1297430:intron 55.0%
GCGACGACGTTGATGCGATC+TGG + chr8_3:57716097-57716116 Msa1297430:CDS 60.0%
CTTCGCAACGTCCTCCGACG+AGG + chr8_3:57716124-57716143 Msa1297430:CDS 65.0%
GTTTCGCTTGGGAGCGGGAG+AGG - chr8_3:57716161-57716180 None:intergenic 65.0%
CGCAACGTCCTCCGACGAGG+AGG + chr8_3:57716127-57716146 Msa1297430:CDS 70.0%
GTCCTCCGACGAGGAGGCTC+CGG + chr8_3:57716133-57716152 Msa1297430:CDS 70.0%
!! AGGCACCGGAGCCTCCTCGT+CGG - chr8_3:57716141-57716160 None:intergenic 70.0%
! CTTGGGAGCGGGAGAGGCAC+CGG - chr8_3:57716155-57716174 None:intergenic 70.0%
CACCGGAGCCTCCTCGTCGG+AGG - chr8_3:57716138-57716157 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 57715990 57719402 57715990 ID=Msa1297430;Name=Msa1297430
chr8_3 mRNA 57715990 57719402 57715990 ID=Msa1297430-mRNA-1;Parent=Msa1297430;Name=Msa1297430-mRNA-1;_AED=0.00;_eAED=0.00;_QI=104|1|1|1|0.66|0.75|4|352|103
chr8_3 exon 57715990 57716214 57715990 ID=Msa1297430-mRNA-1:exon:16525;Parent=Msa1297430-mRNA-1
chr8_3 exon 57716929 57717035 57716929 ID=Msa1297430-mRNA-1:exon:16526;Parent=Msa1297430-mRNA-1
chr8_3 exon 57718077 57718119 57718077 ID=Msa1297430-mRNA-1:exon:16527;Parent=Msa1297430-mRNA-1
chr8_3 exon 57719010 57719402 57719010 ID=Msa1297430-mRNA-1:exon:16528;Parent=Msa1297430-mRNA-1
chr8_3 five_prime_UTR 57715990 57716093 57715990 ID=Msa1297430-mRNA-1:five_prime_utr;Parent=Msa1297430-mRNA-1
chr8_3 CDS 57716094 57716214 57716094 ID=Msa1297430-mRNA-1:cds;Parent=Msa1297430-mRNA-1
chr8_3 CDS 57716929 57717035 57716929 ID=Msa1297430-mRNA-1:cds;Parent=Msa1297430-mRNA-1
chr8_3 CDS 57718077 57718119 57718077 ID=Msa1297430-mRNA-1:cds;Parent=Msa1297430-mRNA-1
chr8_3 CDS 57719010 57719050 57719010 ID=Msa1297430-mRNA-1:cds;Parent=Msa1297430-mRNA-1
chr8_3 three_prime_UTR 57719051 57719402 57719051 ID=Msa1297430-mRNA-1:three_prime_utr;Parent=Msa1297430-mRNA-1
Gene Sequence

>Msa1297430

ATGGCGACGACGTTGATGCGATCTGGTTTTCTTCGCAACGTCCTCCGACGAGGAGGCTCCGGTGCCTCTCCCGCTCCCAAGCGAAACTTTTCTTCCTCCTCTGGTCATCACGATGATGCATATGAAACGGCCAAATGGGAGAAGATTACATATCTAGGCATTGTTTCATGTACCGGTTTAGCTGTTTATACTCTATCAAAGGGCCACCACCACTATGATGAACCTCCTGCATACCCATATTTGCATATCCGCAATAAGGAATTCCCATGGGGTCCAGATGGGCTATTTGAAATCAAGAACGAGCACCACTAG
Protein sequence

>Msa1297430

MATTLMRSGFLRNVLRRGGSGASPAPKRNFSSSSGHHDDAYETAKWEKITYLGIVSCTGLAVYTLSKGHHHYDEPPAYPYLHIRNKEFPWGPDGLFEIKNEHH*