AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0519750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0519750 MtrunA17_Chr3g0135161 94.162 668 26 5 1 665 1 658 0.0 1245
Msa0519750 MtrunA17_Chr1g0152161 60.197 711 184 23 1 664 1 659 0.0 733
Msa0519750 MtrunA17_Chr4g0054351 51.879 692 270 19 1 664 1 657 0.0 544
Msa0519750 MtrunA17_Chr8g0343851 47.984 496 187 11 18 502 3 438 2.03e-137 414
Msa0519750 MtrunA17_Chr5g0400871 46.394 513 187 12 1 501 4 440 5.53e-126 384
Msa0519750 MtrunA17_Chr7g0249621 77.358 212 44 2 302 510 248 458 1.21e-110 348
Msa0519750 MtrunA17_Chr4g0032631 66.800 250 61 4 254 499 122 353 2.57e-110 343
Msa0519750 MtrunA17_Chr4g0072291 62.313 268 86 3 239 491 73 340 3.16e-110 342
Msa0519750 MtrunA17_Chr2g0328971 65.182 247 64 2 254 499 129 354 3.52e-109 339
Msa0519750 MtrunA17_Chr5g0412151 76.768 198 42 2 304 498 215 411 1.47e-95 304
Msa0519750 MtrunA17_Chr4g0073521 71.084 166 48 0 312 477 46 211 9.85e-82 262
Msa0519750 MtrunA17_Chr7g0216601 62.234 188 67 2 293 477 46 232 3.51e-75 246
Msa0519750 MtrunA17_Chr8g0355191 59.512 205 76 2 280 477 15 219 5.94e-75 246
Msa0519750 MtrunA17_Chr6g0452911 58.411 214 71 3 274 477 30 235 5.60e-74 243
Msa0519750 MtrunA17_Chr8g0367311 63.842 177 57 2 350 520 22 197 2.82e-71 231
Msa0519750 MtrunA17_Chr4g0002011 68.047 169 51 1 312 477 35 203 1.95e-70 232
Msa0519750 MtrunA17_Chr8g0355181 59.487 195 70 2 312 506 55 240 1.36e-69 231
Msa0519750 MtrunA17_Chr8g0362541 60.104 193 63 1 299 477 55 247 1.43e-68 229
Msa0519750 MtrunA17_Chr2g0305821 65.574 122 40 1 304 423 36 157 4.48e-44 155
Msa0519750 MtrunA17_Chr4g0053181 53.939 165 68 3 316 480 176 332 1.28e-41 158
Msa0519750 MtrunA17_Chr5g0401891 53.939 165 68 3 316 480 152 308 4.56e-41 157
Msa0519750 MtrunA17_Chr7g0257361 57.237 152 54 3 289 437 36 179 1.27e-40 153
Msa0519750 MtrunA17_Chr7g0263581 50.909 165 73 3 316 480 139 295 1.96e-39 151
Msa0519750 MtrunA17_Chr4g0030191 50.909 165 73 3 316 480 131 287 4.65e-39 150
Msa0519750 MtrunA17_Chr2g0325911 41.410 227 94 8 270 480 72 275 5.72e-32 129
Msa0519750 MtrunA17_Chr2g0305831 43.165 139 62 4 419 542 1 137 6.40e-25 103
Msa0519750 MtrunA17_Chr3g0126151 47.368 95 49 1 361 455 47 140 2.84e-22 93.2
Msa0519750 MtrunA17_Chr8g0367321 85.366 41 6 0 311 351 153 193 1.52e-19 87.4
Msa0519750 MtrunA17_Chr1g0170761 40.426 141 76 3 292 432 113 245 4.09e-17 84.0
Msa0519750 MtrunA17_Chr1g0158971 53.030 66 27 2 407 472 109 170 2.22e-11 65.9
Msa0519750 MtrunA17_Chr8g0375031 53.125 64 29 1 416 479 125 187 2.60e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0519750 AT4G37750.1 52.158 533 151 15 1 496 1 466 2.69e-155 460
Msa0519750 AT1G72570.1 70.370 243 67 3 257 495 164 405 1.97e-117 357
Msa0519750 AT1G51190.1 67.460 252 73 4 254 499 129 377 4.32e-113 352
Msa0519750 AT3G20840.1 68.000 250 69 5 254 499 126 368 7.20e-111 346
Msa0519750 AT5G57390.2 77.387 199 42 1 296 491 65 263 1.43e-110 340
Msa0519750 AT5G57390.1 77.387 199 42 1 296 491 183 381 1.27e-109 342
Msa0519750 AT5G17430.2 67.755 245 74 2 304 545 136 378 3.27e-107 335
Msa0519750 AT5G17430.1 67.755 245 74 2 304 545 198 440 8.47e-107 336
Msa0519750 AT5G10510.2 75.897 195 46 1 304 498 218 411 1.90e-92 297
Msa0519750 AT5G65510.4 67.907 215 66 1 289 500 146 360 1.06e-90 289
Msa0519750 AT5G65510.2 67.907 215 66 1 289 500 204 418 1.32e-90 291
Msa0519750 AT5G65510.1 67.907 215 66 1 289 500 204 418 1.32e-90 291
Msa0519750 AT5G10510.7 74.747 198 46 2 304 498 218 414 2.08e-90 291
Msa0519750 AT5G10510.5 74.747 198 46 2 304 498 218 414 2.08e-90 291
Msa0519750 AT5G10510.1 74.747 198 46 2 304 498 241 437 3.19e-90 292
Msa0519750 AT5G10510.6 74.747 198 46 2 304 498 256 452 4.02e-90 292
Msa0519750 AT5G10510.4 74.747 198 46 2 304 498 256 452 4.02e-90 292
Msa0519750 AT5G10510.3 67.431 218 47 2 304 498 256 472 6.32e-86 281
Msa0519750 AT5G65510.3 70.807 161 44 1 289 446 146 306 1.10e-77 251
Msa0519750 AT5G65510.3 42.667 75 35 1 414 480 172 246 5.08e-11 64.7
Msa0519750 AT3G54320.3 67.241 174 53 2 312 482 61 233 5.48e-69 231
Msa0519750 AT3G54320.1 67.241 174 53 2 312 482 61 233 8.60e-69 231
Msa0519750 AT1G79700.2 68.047 169 51 1 312 477 48 216 2.47e-63 213
Msa0519750 AT1G16060.1 58.373 209 84 1 272 477 14 222 2.63e-62 211
Msa0519750 AT1G79700.1 63.905 169 48 2 312 477 48 206 1.47e-55 191
Msa0519750 AT3G54320.4 69.173 133 40 1 350 482 36 167 1.33e-51 183
Msa0519750 AT3G54320.5 69.173 133 40 1 350 482 36 167 1.33e-51 183
Msa0519750 AT1G79700.3 67.969 128 41 0 350 477 13 140 9.12e-44 157
Msa0519750 AT1G79700.4 67.969 128 41 0 350 477 7 134 9.40e-44 157
Msa0519750 AT4G36920.1 49.500 200 92 4 281 480 97 287 3.14e-42 159
Msa0519750 AT4G36920.2 49.500 200 92 4 281 480 97 287 3.14e-42 159
Msa0519750 AT1G16060.3 67.969 128 41 0 350 477 25 152 4.72e-42 154
Msa0519750 AT5G67180.2 43.534 232 113 4 260 491 50 263 9.25e-42 154
Msa0519750 AT5G67180.3 43.043 230 113 4 260 489 50 261 4.97e-41 151
Msa0519750 AT5G67180.1 43.534 232 113 4 260 491 50 263 5.03e-41 153
Msa0519750 AT2G41710.1 56.579 152 55 3 289 437 46 189 1.64e-40 154
Msa0519750 AT2G41710.4 56.579 152 55 3 289 437 46 189 3.02e-40 154
Msa0519750 AT2G41710.2 54.777 157 55 4 289 437 46 194 4.05e-38 147
Msa0519750 AT2G28550.3 50.303 165 74 3 316 480 153 309 4.99e-38 147
Msa0519750 AT2G41710.3 45.989 187 55 4 289 437 46 224 4.99e-34 135
Msa0519750 AT3G54320.2 70.968 93 26 1 390 482 1 92 7.49e-33 128
Msa0519750 AT1G16060.2 60.909 110 38 1 390 494 1 110 1.00e-31 123
Msa0519750 AT1G16060.4 60.909 110 38 1 390 494 1 110 1.00e-31 123
Msa0519750 AT1G16060.6 60.909 110 38 1 390 494 1 110 1.00e-31 123
Msa0519750 AT1G16060.5 60.909 110 38 1 390 494 1 110 1.00e-31 123
Msa0519750 AT2G28550.4 49.275 138 62 3 316 453 153 282 1.26e-30 123
Msa0519750 AT5G67180.4 39.013 223 117 5 260 482 50 253 2.31e-30 120
Msa0519750 AT2G28550.1 46.061 165 66 4 316 480 153 294 5.30e-30 124
Msa0519750 AT2G28550.2 49.194 124 55 3 316 439 153 268 3.87e-26 108
Msa0519750 AT2G28550.6 49.194 124 55 3 316 439 153 268 3.87e-26 108
Msa0519750 AT2G39250.2 42.202 109 56 2 317 425 109 210 1.31e-22 97.8
Msa0519750 AT2G39250.1 42.202 109 56 2 317 425 109 210 3.77e-22 98.6
Msa0519750 AT2G39250.1 29.368 269 150 12 395 656 88 323 5.90e-11 64.7
Msa0519750 AT2G28550.5 45.802 131 55 2 350 480 14 128 9.73e-22 96.3
Msa0519750 AT5G60120.6 39.181 171 80 6 300 470 145 291 2.13e-19 92.4
Msa0519750 AT5G60120.1 39.181 171 80 6 300 470 145 291 2.24e-19 92.4
Msa0519750 AT5G60120.4 45.238 126 61 3 313 438 157 274 2.33e-19 91.3
Msa0519750 AT5G60120.3 45.238 126 61 3 313 438 157 274 2.33e-19 91.3
Msa0519750 AT5G60120.5 45.238 126 61 3 313 438 157 274 2.33e-19 91.3
Msa0519750 AT5G60120.2 39.181 171 80 6 300 470 145 291 3.56e-19 91.7
Msa0519750 AT3G54990.2 46.154 104 49 2 316 419 122 218 1.57e-17 83.2
Msa0519750 AT3G54990.1 46.154 104 49 2 316 419 122 218 5.23e-17 83.6
Msa0519750 AT1G16060.7 62.025 79 25 1 421 494 10 88 6.45e-17 79.7

Find 146 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGGGTTGGTTGGGAAATT+TGG 0.137392 3_4:+72045952 Msa0519750:three_prime_UTR
TTGGATGATTCTACCAAAAT+AGG 0.146027 3_4:+72045594 Msa0519750:CDS
TGAATGAATGTTAGGACTTT+TGG 0.149737 3_4:+72045983 Msa0519750:three_prime_UTR
GCTAGCAATACTCTTCTTTC+TGG 0.154896 3_4:+72045303 Msa0519750:CDS
CTAATGTTGCTGTTAGTTCT+TGG 0.205616 3_4:+72045739 Msa0519750:CDS
TATCTTTGTTTACTTGAAAA+AGG 0.214410 3_4:-72042567 None:intergenic
AGGAAATCTATTGACACTTT+TGG 0.225909 3_4:+72043700 Msa0519750:CDS
GTAGATATGAAGCACATTTA+TGG 0.228782 3_4:+72043944 Msa0519750:CDS
CCTCTAATGGTGCTTGAAAT+AGG 0.284929 3_4:-72043351 None:intergenic
CTGAAGAAGTTTGAGTTTGT+TGG 0.307916 3_4:-72042721 None:intergenic
AAGCCATTGCCTCACTTTCA+TGG 0.308137 3_4:-72043186 None:intergenic
CTGCAAAACCATCTTCCATT+TGG 0.319566 3_4:-72045457 None:intergenic
TGTTATGGTGTTCCTGAAAA+TGG 0.323560 3_4:+72042806 Msa0519750:CDS
AACTCAAGGTGGTTATGATA+TGG 0.323727 3_4:+72044265 Msa0519750:intron
TCTTTAGATTCTACCTCTAA+TGG 0.324936 3_4:-72043364 None:intergenic
CCAAGAAAATCCTCTAGTTT+AGG 0.328835 3_4:-72043130 None:intergenic
TTTGATTCCATTGAAGGCTT+TGG 0.329887 3_4:-72045708 None:intergenic
TATTCTCCATTAGGCATAGA+TGG 0.336451 3_4:+72043917 Msa0519750:intron
TAACCAAGTGCTGCATAATA+TGG 0.342134 3_4:-72043322 None:intergenic
TCATTTCCTCAAGTTGAGTA+TGG 0.344608 3_4:-72044468 None:intergenic
ATAGGCATAACAGTCAAATT+AGG 0.347664 3_4:-72042845 None:intergenic
TCAAGCAGCTCTTAAGTATT+GGG 0.347974 3_4:+72044313 Msa0519750:CDS
TATATATATGTTTGTTTGGT+TGG 0.349105 3_4:+72042353 Msa0519750:five_prime_UTR
AGGATTAATGAATGAATGTT+AGG 0.358146 3_4:+72045975 Msa0519750:three_prime_UTR
CCTATTTCAAGCACCATTAG+AGG 0.368785 3_4:+72043351 Msa0519750:CDS
CCTAGACAGATCTCACCTTC+TGG 0.379310 3_4:+72043607 Msa0519750:CDS
CCTTAACAAACTTAGCCTTT+TGG 0.380384 3_4:+72046047 Msa0519750:three_prime_UTR
CGCTTCAGCAGCTTCTCCAT+TGG 0.384097 3_4:-72045409 None:intergenic
AGAGTTACTACTGTCATTCA+TGG 0.391896 3_4:-72042612 None:intergenic
CAGCAGCTTCTCCATTGGTC+TGG 0.395700 3_4:-72045404 None:intergenic
GGTAACAAGGACCTTTATCT+TGG 0.399524 3_4:+72044812 Msa0519750:CDS
GTTCCCAAATGCCTCAAAAC+TGG 0.400777 3_4:+72043413 Msa0519750:CDS
AATCATAACAACAATTGGTT+AGG 0.403766 3_4:+72042644 Msa0519750:CDS
TAGATATGAAGCACATTTAT+GGG 0.403876 3_4:+72043945 Msa0519750:CDS
TGGTATTAATTCAGTTGGTT+TGG 0.404298 3_4:+72045575 Msa0519750:CDS
GAAGAGAAATAGATTCTGTT+AGG 0.405652 3_4:+72042527 Msa0519750:five_prime_UTR
GCAAGGATTGGAAGAGTTGC+TGG 0.408354 3_4:+72044791 Msa0519750:CDS
ACTCTTTCCAAAGCCTTCAA+TGG 0.408843 3_4:+72045701 Msa0519750:CDS
TGGAACAGAATCAGTGACTA+TGG 0.411439 3_4:+72043627 Msa0519750:CDS
ACAACAATCATAACAACAAT+TGG 0.411645 3_4:+72042639 Msa0519750:CDS
TACTGAATGTTCCAAGATAA+AGG 0.412580 3_4:-72044823 None:intergenic
AGTTTATATATATGTTTGTT+TGG 0.414467 3_4:+72042349 Msa0519750:five_prime_UTR
TGGATGATTCTACCAAAATA+GGG 0.417120 3_4:+72045595 Msa0519750:CDS
AAGGAAGGACAAACAAGAAA+AGG 0.424488 3_4:+72043982 Msa0519750:CDS
TTCAACACACATAAACTTCC+CGG 0.425153 3_4:+72044340 Msa0519750:CDS
TATGCAGCACTTGGTTATCA+TGG 0.425353 3_4:+72043328 Msa0519750:CDS
CCAAAAGGCTAAGTTTGTTA+AGG 0.429179 3_4:-72046047 None:intergenic
ACTAGCATGGGGAGTCATAA+TGG 0.434049 3_4:+72043475 Msa0519750:CDS
AGGAGAAGAAGTAGAAATGT+GGG 0.436963 3_4:-72042681 None:intergenic
TCAATAGATTTCCTATGAAT+AGG 0.438789 3_4:-72043691 None:intergenic
ACAAACTCAACAATCTGTTA+TGG 0.440794 3_4:+72042791 Msa0519750:CDS
ATGAATGAGAATATGCATAT+TGG 0.443517 3_4:+72046082 Msa0519750:three_prime_UTR
ATCAAGCAGCTCTTAAGTAT+TGG 0.446724 3_4:+72044312 Msa0519750:CDS
TCTTCTGTTGTTCGTACTGT+AGG 0.447306 3_4:+72043517 Msa0519750:CDS
TTCTTTCTGGTCAGCATGCA+AGG 0.453728 3_4:+72045316 Msa0519750:CDS
AGTGATCCATCTGACTTGAT+AGG 0.454921 3_4:-72042863 None:intergenic
AAACTTGTCACCAATGATGA+TGG 0.469910 3_4:-72045633 None:intergenic
TAATGATTCCAAATGGAAGA+TGG 0.471281 3_4:+72045449 Msa0519750:CDS
ATGTCTTTACAAGACCTTGT+TGG 0.474882 3_4:+72045555 Msa0519750:CDS
CTTTGTATCATGGAAGCTCT+TGG 0.479184 3_4:+72042884 Msa0519750:CDS
AAAATCATTGTGAATTCTGA+TGG 0.483714 3_4:+72045507 Msa0519750:CDS
GGGAAGTTTATGTGTGTTGA+AGG 0.488674 3_4:-72044338 None:intergenic
CTTGTTGGTATTAATTCAGT+TGG 0.489454 3_4:+72045570 Msa0519750:CDS
AGAACTAACAGCAACATTAG+TGG 0.490687 3_4:-72045736 None:intergenic
ACGAAAATAATGATTCCAAA+TGG 0.491698 3_4:+72045442 Msa0519750:CDS
GTCAAAAGCAGCCTATTCAT+AGG 0.492472 3_4:+72043680 Msa0519750:CDS
GGTAGGTGGCAAGCAAGGAT+TGG 0.507927 3_4:+72044779 Msa0519750:CDS
CATCCATATTATGCAGCACT+TGG 0.508376 3_4:+72043319 Msa0519750:CDS
TTTCTTGGTGGTGCAACAAT+GGG 0.508995 3_4:+72043145 Msa0519750:CDS
GGGTTGGTTGGGAAATTTGG+AGG 0.511636 3_4:+72045955 Msa0519750:three_prime_UTR
AGATGGATCACTTTGTATCA+TGG 0.512780 3_4:+72042874 Msa0519750:CDS
ACACAGCTAGATTGAGAACC+AGG 0.513570 3_4:-72043577 None:intergenic
GTTGTTCGTACTGTAGGAAG+TGG 0.513611 3_4:+72043523 Msa0519750:CDS
AGTCATAATGGTGGAGCTGG+TGG 0.513615 3_4:+72043487 Msa0519750:CDS
TCCATTAGGCATAGATGGAC+TGG 0.517509 3_4:+72043922 Msa0519750:intron
ATATGATGTTGAAAGGATCA+TGG 0.520797 3_4:+72045281 Msa0519750:CDS
AATGAGAATATGCATATTGG+AGG 0.520951 3_4:+72046085 Msa0519750:three_prime_UTR
CATAATGGTGGAGCTGGTGG+TGG 0.521042 3_4:+72043490 Msa0519750:CDS
GAGGAGAAGAAGTAGAAATG+TGG 0.523031 3_4:-72042682 None:intergenic
GGGAGTCATAATGGTGGAGC+TGG 0.523283 3_4:+72043484 Msa0519750:CDS
CAAGGAGATATAATCTCTCT+AGG 0.523503 3_4:-72045847 None:intergenic
GTCTGAAAGATTCTGAAAGA+AGG 0.527254 3_4:-72043285 None:intergenic
GGCTTTGGAAAGAGTAGTGA+GGG 0.530598 3_4:-72045693 None:intergenic
AAAAGAGGAGCTGCTAAGCT+TGG 0.531048 3_4:+72043658 Msa0519750:CDS
AAGCTGAAGCAGAAGAATAG+AGG 0.531685 3_4:+72042424 Msa0519750:five_prime_UTR
GAGAACCAGGGCTCATTGAA+AGG 0.533035 3_4:-72043564 None:intergenic
CCAGAAGGTGAGATCTGTCT+AGG 0.534044 3_4:-72043607 None:intergenic
TATCTAAATATGATGTTGAA+AGG 0.534738 3_4:+72045274 Msa0519750:CDS
CATTCCAACATATAGCCAAA+AGG 0.534863 3_4:-72046062 None:intergenic
TATTCATGAACTTCGGTCTG+TGG 0.536183 3_4:-72045357 None:intergenic
GTTGAGTTTGTGAAGTGAGA+AGG 0.536370 3_4:-72042779 None:intergenic
GACAAATTGATTGAAGATGC+TGG 0.538955 3_4:-72045792 None:intergenic
TTAAGCCTTTCAATGAGCCC+TGG 0.541546 3_4:+72043559 Msa0519750:CDS
CTGAAAGAAGGTCAAGGGAA+TGG 0.549375 3_4:-72043273 None:intergenic
ATGGGATAACAGTTGCAAGA+AGG 0.549719 3_4:+72043963 Msa0519750:CDS
ACAATGGGTAGTGATGAATA+TGG 0.550458 3_4:+72043160 Msa0519750:CDS
CAGAGAACATCTCAGTATAG+AGG 0.550832 3_4:+72043724 Msa0519750:CDS
TGCAAGGAGAATCAAAGATA+AGG 0.550976 3_4:+72045332 Msa0519750:CDS
ATTTGTGTAGCTACTTGTGA+AGG 0.555126 3_4:-72045765 None:intergenic
TTGCTTGCCACCTACCATGT+TGG 0.556430 3_4:-72044772 None:intergenic
ATGATTATATTCATGAACTT+CGG 0.556488 3_4:-72045364 None:intergenic
AATGAAGAATATGACAAGGC+AGG 0.556697 3_4:+72044486 Msa0519750:CDS
CAGATGAACACTACTAGCAT+GGG 0.557648 3_4:+72043463 Msa0519750:CDS
TGGAAGAGTTGCTGGTAACA+AGG 0.559839 3_4:+72044799 Msa0519750:CDS
GCAGATGAACACTACTAGCA+TGG 0.560674 3_4:+72043462 Msa0519750:CDS
TTGTAACAGCATTTGCTCCA+CGG 0.565794 3_4:-72045242 None:intergenic
AGGCTTTGGAAAGAGTAGTG+AGG 0.568883 3_4:-72045694 None:intergenic
AAGATTCTGAAAGAAGGTCA+AGG 0.578912 3_4:-72043279 None:intergenic
CACAAGTAGCTACACAAATG+AGG 0.580768 3_4:+72045769 Msa0519750:CDS
GTTATGCCTATCAAGTCAGA+TGG 0.582455 3_4:+72042857 Msa0519750:CDS
AATATGTTGCACATTTGAGA+AGG 0.587308 3_4:+72044509 Msa0519750:CDS
TAGCCATGAAAGTGAGGCAA+TGG 0.587506 3_4:+72043183 Msa0519750:CDS
GAAAGAAGCAGGAACCGTGT+TGG 0.594498 3_4:-72042747 None:intergenic
AAACTACCATACTCAACTTG+AGG 0.599024 3_4:+72044462 Msa0519750:CDS
ATCTTCATCTCCTAAACTAG+AGG 0.599319 3_4:+72043120 Msa0519750:CDS
AACATGGTAGGTGGCAAGCA+AGG 0.600209 3_4:+72044774 Msa0519750:CDS
CAATGTACAGAGGCGTGACA+AGG 0.600518 3_4:+72044656 Msa0519750:CDS
GTCACTGATTCTGTTCCAGA+AGG 0.600695 3_4:-72043622 None:intergenic
GCAGACATCACCAACATGGT+AGG 0.604873 3_4:+72044762 Msa0519750:intron
ATGTAACACAAGGGTAACAT+TGG 0.605982 3_4:+72046024 Msa0519750:three_prime_UTR
AACTTCTTCAGTTTCCAACA+CGG 0.606767 3_4:+72042733 Msa0519750:CDS
AGATTCTGAAAGAAGGTCAA+GGG 0.611616 3_4:-72043278 None:intergenic
CTCAGTATAGAGGTGTCACA+AGG 0.617014 3_4:+72043734 Msa0519750:CDS
CAAGCAGCTCTTAAGTATTG+GGG 0.623947 3_4:+72044314 Msa0519750:CDS
CAAACAAGAAAAGGAAGACA+AGG 0.624646 3_4:+72043991 Msa0519750:CDS
AATGTATCGAGCCAGACCAA+TGG 0.631591 3_4:+72045393 Msa0519750:CDS
TCTAGTTTAGGAGATGAAGA+TGG 0.633764 3_4:-72043118 None:intergenic
GACATCACCAACATGGTAGG+TGG 0.639099 3_4:+72044765 Msa0519750:intron
ATGAGAATATGCATATTGGA+GGG 0.640086 3_4:+72046086 Msa0519750:three_prime_UTR
TGGTCTGTATTAGCTTGATG+AGG 0.640420 3_4:-72043253 None:intergenic
GATAACAGTTGCAAGAAGGA+AGG 0.640507 3_4:+72043967 Msa0519750:CDS
AAAAGCAGTGGATTCTCAAG+GGG 0.641907 3_4:+72044628 Msa0519750:CDS
ATATGGTAGCCATGAAAGTG+AGG 0.643799 3_4:+72043177 Msa0519750:CDS
CTTGGTAGATCACAATCACA+AGG 0.655160 3_4:+72042902 Msa0519750:CDS
TTTGTCATAACATAGTAACA+AGG 0.658655 3_4:-72045865 None:intergenic
GTTACATAAAGAAAACAAAG+TGG 0.660708 3_4:-72046008 None:intergenic
CAACTGAATTAATACCAACA+AGG 0.665562 3_4:-72045569 None:intergenic
GTAGCAGCAATAAAATTCCG+TGG 0.666673 3_4:+72045225 Msa0519750:CDS
GGAGAAGAAGTAGAAATGTG+GGG 0.675273 3_4:-72042680 None:intergenic
AACAGAATCAGTGACTATGG+AGG 0.680741 3_4:+72043630 Msa0519750:CDS
AGCATGGGGAGTCATAATGG+TGG 0.689715 3_4:+72043478 Msa0519750:CDS
AGGAAATGAAGAATATGACA+AGG 0.693051 3_4:+72044482 Msa0519750:CDS
GGGAACTAGAAACATCAACA+TGG 0.697253 3_4:-72043396 None:intergenic
CACAGCTAGATTGAGAACCA+GGG 0.711589 3_4:-72043576 None:intergenic
AGATGAACACTACTAGCATG+GGG 0.712889 3_4:+72043464 Msa0519750:CDS
AGGGGTGCATCAATGTACAG+AGG 0.720794 3_4:+72044646 Msa0519750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAATAACAACTTTTTTT+TGG - chr3_4:72043015-72043034 None:intergenic 10.0%
!! AAAGAAAAAAAATTACAAAA+AGG - chr3_4:72045154-72045173 None:intergenic 10.0%
!!! GGATATTTATTTTTTAAAAA+GGG + chr3_4:72045935-72045954 Msa0519750:three_prime_UTR 10.0%
!! AGTTTATATATATGTTTGTT+TGG + chr3_4:72042349-72042368 Msa0519750:five_prime_UTR 15.0%
!! TCTTTAATTAATGTTAGATA+TGG + chr3_4:72043037-72043056 Msa0519750:intron 15.0%
!!! TGTTAGTATAAAATTTTAGT+AGG - chr3_4:72044062-72044081 None:intergenic 15.0%
!! ACAAATTCAAATGAAAAAAA+GGG - chr3_4:72044700-72044719 None:intergenic 15.0%
!!! TTGTAATTTTTTTTCTTTCT+TGG + chr3_4:72045156-72045175 Msa0519750:intron 15.0%
!! TATGTTATGACAAAAAAAAA+GGG + chr3_4:72045874-72045893 Msa0519750:three_prime_UTR 15.0%
!! ATTATGTATTGAAAAACATA+TGG + chr3_4:72045912-72045931 Msa0519750:three_prime_UTR 15.0%
!! TTATGTATTGAAAAACATAT+GGG + chr3_4:72045913-72045932 Msa0519750:three_prime_UTR 15.0%
!!! GGGATATTTATTTTTTAAAA+AGG + chr3_4:72045934-72045953 Msa0519750:three_prime_UTR 15.0%
!!! ATTTATTTTTTAAAAAGGGT+TGG + chr3_4:72045939-72045958 Msa0519750:three_prime_UTR 15.0%
!! TATATATATGTTTGTTTGGT+TGG + chr3_4:72042353-72042372 Msa0519750:five_prime_UTR 20.0%
!!! TATCTTTGTTTACTTGAAAA+AGG - chr3_4:72042570-72042589 None:intergenic 20.0%
!!! AAATAACAACTTTTTTTTGG+TGG - chr3_4:72043012-72043031 None:intergenic 20.0%
!! AAAACAACATAATCATTACA+GGG - chr3_4:72043063-72043082 None:intergenic 20.0%
!! TTATTATATGATTGAAGTGA+TGG + chr3_4:72043093-72043112 Msa0519750:intron 20.0%
!!! TGTTTGTTTTTTCTTTTACT+TGG - chr3_4:72044177-72044196 None:intergenic 20.0%
!!! GCAATTATTTTGTAGTAAAA+AGG + chr3_4:72044551-72044570 Msa0519750:intron 20.0%
!! TAATTATATGACAATCATGA+TGG + chr3_4:72044580-72044599 Msa0519750:intron 20.0%
!! GACAAATTCAAATGAAAAAA+AGG - chr3_4:72044701-72044720 None:intergenic 20.0%
!!! TTTAAAGTAAAATCCTTCAT+AGG + chr3_4:72044949-72044968 Msa0519750:intron 20.0%
!! AGTAAATTAAATTCCTATGA+AGG - chr3_4:72044965-72044984 None:intergenic 20.0%
!!! TTTCTTGGAAATGAAATTTT+AGG + chr3_4:72045171-72045190 Msa0519750:intron 20.0%
!! TATCTAAATATGATGTTGAA+AGG + chr3_4:72045274-72045293 Msa0519750:CDS 20.0%
!! ATGATTATATTCATGAACTT+CGG - chr3_4:72045367-72045386 None:intergenic 20.0%
!! CTATGTTATGACAAAAAAAA+AGG + chr3_4:72045873-72045892 Msa0519750:three_prime_UTR 20.0%
!! ACAAAAAAAAAGGGTAAAAA+GGG + chr3_4:72045883-72045902 Msa0519750:three_prime_UTR 20.0%
!! TATGTATTGAAAAACATATG+GGG + chr3_4:72045914-72045933 Msa0519750:three_prime_UTR 20.0%
! ACAACAATCATAACAACAAT+TGG + chr3_4:72042639-72042658 Msa0519750:CDS 25.0%
! AATCATAACAACAATTGGTT+AGG + chr3_4:72042644-72042663 Msa0519750:CDS 25.0%
! GAAAACAACATAATCATTAC+AGG - chr3_4:72043064-72043083 None:intergenic 25.0%
! TCAATAGATTTCCTATGAAT+AGG - chr3_4:72043694-72043713 None:intergenic 25.0%
!!! GATTTTGTTTATTCTCCATT+AGG + chr3_4:72043908-72043927 Msa0519750:intron 25.0%
! TAGATATGAAGCACATTTAT+GGG + chr3_4:72043945-72043964 Msa0519750:CDS 25.0%
! ATGCATGTTACAGTATATTT+GGG + chr3_4:72044129-72044148 Msa0519750:intron 25.0%
!!! TTTTTTCTTTTACTTGGTGA+AGG - chr3_4:72044171-72044190 None:intergenic 25.0%
!!! GTTTCTAATTTTGACTTTGT+TGG + chr3_4:72044230-72044249 Msa0519750:intron 25.0%
!!! GGTTTTATTTTTTGAACTCA+AGG + chr3_4:72044251-72044270 Msa0519750:intron 25.0%
!!! TTTATTTTTTGAACTCAAGG+TGG + chr3_4:72044254-72044273 Msa0519750:intron 25.0%
!!! ATTTTTAGTAACAATGTTGG+AGG - chr3_4:72044857-72044876 None:intergenic 25.0%
!!! CACATTTTTAGTAACAATGT+TGG - chr3_4:72044860-72044879 None:intergenic 25.0%
! AAGCATTTGAATTATGAACA+AGG + chr3_4:72044894-72044913 Msa0519750:intron 25.0%
! AGCATTTGAATTATGAACAA+GGG + chr3_4:72044895-72044914 Msa0519750:intron 25.0%
!!! TGATTGGTTATGCTTTTATA+AGG + chr3_4:72045027-72045046 Msa0519750:intron 25.0%
!!! ACAAAATTCCACTTTTTCTA+AGG + chr3_4:72045073-72045092 Msa0519750:intron 25.0%
! ACGAAAATAATGATTCCAAA+TGG + chr3_4:72045442-72045461 Msa0519750:CDS 25.0%
! AAAATCATTGTGAATTCTGA+TGG + chr3_4:72045507-72045526 Msa0519750:CDS 25.0%
!!! TTTTTCAAATCCATCATCAT+TGG + chr3_4:72045623-72045642 Msa0519750:CDS 25.0%
! TTTGTCATAACATAGTAACA+AGG - chr3_4:72045868-72045887 None:intergenic 25.0%
! GACAAAAAAAAAGGGTAAAA+AGG + chr3_4:72045882-72045901 Msa0519750:three_prime_UTR 25.0%
!!! ATTTTTTAAAAAGGGTTGGT+TGG + chr3_4:72045943-72045962 Msa0519750:three_prime_UTR 25.0%
!!! TTTTTTAAAAAGGGTTGGTT+GGG + chr3_4:72045944-72045963 Msa0519750:three_prime_UTR 25.0%
! AGGATTAATGAATGAATGTT+AGG + chr3_4:72045975-72045994 Msa0519750:three_prime_UTR 25.0%
! GTTACATAAAGAAAACAAAG+TGG - chr3_4:72046011-72046030 None:intergenic 25.0%
!! TGTTTTCTTTATGTAACACA+AGG + chr3_4:72046014-72046033 Msa0519750:three_prime_UTR 25.0%
!! GTTTTCTTTATGTAACACAA+GGG + chr3_4:72046015-72046034 Msa0519750:three_prime_UTR 25.0%
! ATGAATGAGAATATGCATAT+TGG + chr3_4:72046082-72046101 Msa0519750:three_prime_UTR 25.0%
GAAGAGAAATAGATTCTGTT+AGG + chr3_4:72042527-72042546 Msa0519750:five_prime_UTR 30.0%
ACAAACTCAACAATCTGTTA+TGG + chr3_4:72042791-72042810 Msa0519750:CDS 30.0%
! TGAAAGTTGTTACCATTTTC+AGG - chr3_4:72042821-72042840 None:intergenic 30.0%
ATAGGCATAACAGTCAAATT+AGG - chr3_4:72042848-72042867 None:intergenic 30.0%
TCTTTAGATTCTACCTCTAA+TGG - chr3_4:72043367-72043386 None:intergenic 30.0%
! AGGAAATCTATTGACACTTT+TGG + chr3_4:72043700-72043719 Msa0519750:CDS 30.0%
GCTTTCTTAAGTTATCATGT+TGG + chr3_4:72043873-72043892 Msa0519750:intron 30.0%
!! ATCATGTTGGTGATTATCTT+TGG + chr3_4:72043886-72043905 Msa0519750:intron 30.0%
GTAGATATGAAGCACATTTA+TGG + chr3_4:72043944-72043963 Msa0519750:CDS 30.0%
!!! GTGTTATTTGTTCATGCTTT+TGG + chr3_4:72044083-72044102 Msa0519750:intron 30.0%
CATGCATGTTACAGTATATT+TGG + chr3_4:72044128-72044147 Msa0519750:intron 30.0%
AGAAACAGTAATAGCAATGA+TGG - chr3_4:72044216-72044235 None:intergenic 30.0%
AAGCTAACAAGAAAATGCTA+AGG - chr3_4:72044378-72044397 None:intergenic 30.0%
AGGAAATGAAGAATATGACA+AGG + chr3_4:72044482-72044501 Msa0519750:CDS 30.0%
AATATGTTGCACATTTGAGA+AGG + chr3_4:72044509-72044528 Msa0519750:CDS 30.0%
TATTATTGCAGAAAAAGCAG+TGG + chr3_4:72044616-72044635 Msa0519750:intron 30.0%
ATTTGAATTTGTCACACTGT+TGG + chr3_4:72044708-72044727 Msa0519750:intron 30.0%
TACTGAATGTTCCAAGATAA+AGG - chr3_4:72044826-72044845 None:intergenic 30.0%
ATAACCAATCACTTGTGTTT+GGG - chr3_4:72045018-72045037 None:intergenic 30.0%
!!! ACTTTTTCTAAGGCTGTAAA+GGG + chr3_4:72045083-72045102 Msa0519750:intron 30.0%
AAAGGGAATCTCTAATAGAA+TGG + chr3_4:72045100-72045119 Msa0519750:intron 30.0%
ATCTCTAATAGAATGGTGTA+AGG + chr3_4:72045107-72045126 Msa0519750:intron 30.0%
ATATGATGTTGAAAGGATCA+TGG + chr3_4:72045281-72045300 Msa0519750:CDS 30.0%
TAATGATTCCAAATGGAAGA+TGG + chr3_4:72045449-72045468 Msa0519750:CDS 30.0%
CAACTGAATTAATACCAACA+AGG - chr3_4:72045572-72045591 None:intergenic 30.0%
CTTGTTGGTATTAATTCAGT+TGG + chr3_4:72045570-72045589 Msa0519750:CDS 30.0%
TGGTATTAATTCAGTTGGTT+TGG + chr3_4:72045575-72045594 Msa0519750:CDS 30.0%
TTGGATGATTCTACCAAAAT+AGG + chr3_4:72045594-72045613 Msa0519750:CDS 30.0%
TGGATGATTCTACCAAAATA+GGG + chr3_4:72045595-72045614 Msa0519750:CDS 30.0%
! TGAAAAATGTGTCCCTATTT+TGG - chr3_4:72045610-72045629 None:intergenic 30.0%
!! GCAATTTTTGATTCCATTGA+AGG - chr3_4:72045717-72045736 None:intergenic 30.0%
! TGAATGAATGTTAGGACTTT+TGG + chr3_4:72045983-72046002 Msa0519750:three_prime_UTR 30.0%
! GAATGAATGTTAGGACTTTT+GGG + chr3_4:72045984-72046003 Msa0519750:three_prime_UTR 30.0%
! AATGAATGTTAGGACTTTTG+GGG + chr3_4:72045985-72046004 Msa0519750:three_prime_UTR 30.0%
AGAGTTACTACTGTCATTCA+TGG - chr3_4:72042615-72042634 None:intergenic 35.0%
AGGAGAAGAAGTAGAAATGT+GGG - chr3_4:72042684-72042703 None:intergenic 35.0%
! TGGTAATGATGATGATGATG+AGG - chr3_4:72042704-72042723 None:intergenic 35.0%
! CTGAAGAAGTTTGAGTTTGT+TGG - chr3_4:72042724-72042743 None:intergenic 35.0%
AACTTCTTCAGTTTCCAACA+CGG + chr3_4:72042733-72042752 Msa0519750:CDS 35.0%
GGTGAAAAATAGAAAGAAGC+AGG - chr3_4:72042761-72042780 None:intergenic 35.0%
TGTTATGGTGTTCCTGAAAA+TGG + chr3_4:72042806-72042825 Msa0519750:CDS 35.0%
AGATGGATCACTTTGTATCA+TGG + chr3_4:72042874-72042893 Msa0519750:CDS 35.0%
! AGCACACAACACATTTTGAA+GGG - chr3_4:72042966-72042985 None:intergenic 35.0%
! AAGCACACAACACATTTTGA+AGG - chr3_4:72042967-72042986 None:intergenic 35.0%
! TCTAGTTTAGGAGATGAAGA+TGG - chr3_4:72043121-72043140 None:intergenic 35.0%
ATCTTCATCTCCTAAACTAG+AGG + chr3_4:72043120-72043139 Msa0519750:CDS 35.0%
! CCAAGAAAATCCTCTAGTTT+AGG - chr3_4:72043133-72043152 None:intergenic 35.0%
! CCTAAACTAGAGGATTTTCT+TGG + chr3_4:72043130-72043149 Msa0519750:CDS 35.0%
! AAACTAGAGGATTTTCTTGG+TGG + chr3_4:72043133-72043152 Msa0519750:CDS 35.0%
ACAATGGGTAGTGATGAATA+TGG + chr3_4:72043160-72043179 Msa0519750:CDS 35.0%
! AGATTCTGAAAGAAGGTCAA+GGG - chr3_4:72043281-72043300 None:intergenic 35.0%
! AAGATTCTGAAAGAAGGTCA+AGG - chr3_4:72043282-72043301 None:intergenic 35.0%
! GTCTGAAAGATTCTGAAAGA+AGG - chr3_4:72043288-72043307 None:intergenic 35.0%
! TAACCAAGTGCTGCATAATA+TGG - chr3_4:72043325-72043344 None:intergenic 35.0%
!!! ATACCAGTTTTGAGGCATTT+GGG - chr3_4:72043419-72043438 None:intergenic 35.0%
!!! AATACCAGTTTTGAGGCATT+TGG - chr3_4:72043420-72043439 None:intergenic 35.0%
ACTATGGAGGCTAAAAAAAG+AGG + chr3_4:72043643-72043662 Msa0519750:CDS 35.0%
TATTCTCCATTAGGCATAGA+TGG + chr3_4:72043917-72043936 Msa0519750:intron 35.0%
AAGGAAGGACAAACAAGAAA+AGG + chr3_4:72043982-72044001 Msa0519750:CDS 35.0%
CAAACAAGAAAAGGAAGACA+AGG + chr3_4:72043991-72044010 Msa0519750:CDS 35.0%
AACTCAAGGTGGTTATGATA+TGG + chr3_4:72044265-72044284 Msa0519750:intron 35.0%
ATCAAGCAGCTCTTAAGTAT+TGG + chr3_4:72044312-72044331 Msa0519750:CDS 35.0%
TCAAGCAGCTCTTAAGTATT+GGG + chr3_4:72044313-72044332 Msa0519750:CDS 35.0%
TTCAACACACATAAACTTCC+CGG + chr3_4:72044340-72044359 Msa0519750:CDS 35.0%
AAACTACCATACTCAACTTG+AGG + chr3_4:72044462-72044481 Msa0519750:CDS 35.0%
!! TCATTTCCTCAAGTTGAGTA+TGG - chr3_4:72044471-72044490 None:intergenic 35.0%
AATGAAGAATATGACAAGGC+AGG + chr3_4:72044486-72044505 Msa0519750:CDS 35.0%
! AAAAAGCAGTGGATTCTCAA+GGG + chr3_4:72044627-72044646 Msa0519750:CDS 35.0%
! TTTTCCCAAACACAAGTGAT+TGG + chr3_4:72045011-72045030 Msa0519750:intron 35.0%
CATAACCAATCACTTGTGTT+TGG - chr3_4:72045019-72045038 None:intergenic 35.0%
CTTTACAGCCTTAGAAAAAG+TGG - chr3_4:72045084-72045103 None:intergenic 35.0%
!!! CACTTTTTCTAAGGCTGTAA+AGG + chr3_4:72045082-72045101 Msa0519750:intron 35.0%
! TGAAATTTTAGGCACTCAAG+AGG + chr3_4:72045182-72045201 Msa0519750:intron 35.0%
TGCAAGGAGAATCAAAGATA+AGG + chr3_4:72045332-72045351 Msa0519750:CDS 35.0%
ATGTCTTTACAAGACCTTGT+TGG + chr3_4:72045555-72045574 Msa0519750:CDS 35.0%
AAACTTGTCACCAATGATGA+TGG - chr3_4:72045636-72045655 None:intergenic 35.0%
TTTGATTCCATTGAAGGCTT+TGG - chr3_4:72045711-72045730 None:intergenic 35.0%
AGAACTAACAGCAACATTAG+TGG - chr3_4:72045739-72045758 None:intergenic 35.0%
! CTAATGTTGCTGTTAGTTCT+TGG + chr3_4:72045739-72045758 Msa0519750:CDS 35.0%
ATTTGTGTAGCTACTTGTGA+AGG - chr3_4:72045768-72045787 None:intergenic 35.0%
GACAAATTGATTGAAGATGC+TGG - chr3_4:72045795-72045814 None:intergenic 35.0%
CAAGGAGATATAATCTCTCT+AGG - chr3_4:72045850-72045869 None:intergenic 35.0%
ATGTAACACAAGGGTAACAT+TGG + chr3_4:72046024-72046043 Msa0519750:three_prime_UTR 35.0%
!! CCAAAAGGCTAAGTTTGTTA+AGG - chr3_4:72046050-72046069 None:intergenic 35.0%
!! CCTTAACAAACTTAGCCTTT+TGG + chr3_4:72046047-72046066 Msa0519750:three_prime_UTR 35.0%
!! TTAGCCTTTTGGCTATATGT+TGG + chr3_4:72046058-72046077 Msa0519750:three_prime_UTR 35.0%
CATTCCAACATATAGCCAAA+AGG - chr3_4:72046065-72046084 None:intergenic 35.0%
AAGCTGAAGCAGAAGAATAG+AGG + chr3_4:72042424-72042443 Msa0519750:five_prime_UTR 40.0%
GGAGAAGAAGTAGAAATGTG+GGG - chr3_4:72042683-72042702 None:intergenic 40.0%
GAGGAGAAGAAGTAGAAATG+TGG - chr3_4:72042685-72042704 None:intergenic 40.0%
GTTGAGTTTGTGAAGTGAGA+AGG - chr3_4:72042782-72042801 None:intergenic 40.0%
GTTATGCCTATCAAGTCAGA+TGG + chr3_4:72042857-72042876 Msa0519750:CDS 40.0%
! AGTGATCCATCTGACTTGAT+AGG - chr3_4:72042866-72042885 None:intergenic 40.0%
CTTTGTATCATGGAAGCTCT+TGG + chr3_4:72042884-72042903 Msa0519750:CDS 40.0%
CTTGGTAGATCACAATCACA+AGG + chr3_4:72042902-72042921 Msa0519750:CDS 40.0%
GTAGATCACAATCACAAGGT+TGG + chr3_4:72042906-72042925 Msa0519750:intron 40.0%
!!! TTTTCTTGGTGGTGCAACAA+TGG + chr3_4:72043144-72043163 Msa0519750:CDS 40.0%
!! TTTCTTGGTGGTGCAACAAT+GGG + chr3_4:72043145-72043164 Msa0519750:CDS 40.0%
ATATGGTAGCCATGAAAGTG+AGG + chr3_4:72043177-72043196 Msa0519750:CDS 40.0%
! GATGAGGATCTGCATTTTGT+TGG - chr3_4:72043240-72043259 None:intergenic 40.0%
! TGGTCTGTATTAGCTTGATG+AGG - chr3_4:72043256-72043275 None:intergenic 40.0%
CATCCATATTATGCAGCACT+TGG + chr3_4:72043319-72043338 Msa0519750:CDS 40.0%
TATGCAGCACTTGGTTATCA+TGG + chr3_4:72043328-72043347 Msa0519750:CDS 40.0%
CCTCTAATGGTGCTTGAAAT+AGG - chr3_4:72043354-72043373 None:intergenic 40.0%
CCTATTTCAAGCACCATTAG+AGG + chr3_4:72043351-72043370 Msa0519750:CDS 40.0%
GGGAACTAGAAACATCAACA+TGG - chr3_4:72043399-72043418 None:intergenic 40.0%
! GAAGCAGAATACCAGTTTTG+AGG - chr3_4:72043427-72043446 None:intergenic 40.0%
CAGATGAACACTACTAGCAT+GGG + chr3_4:72043463-72043482 Msa0519750:CDS 40.0%
AGATGAACACTACTAGCATG+GGG + chr3_4:72043464-72043483 Msa0519750:CDS 40.0%
TCTTCTGTTGTTCGTACTGT+AGG + chr3_4:72043517-72043536 Msa0519750:CDS 40.0%
TGGAACAGAATCAGTGACTA+TGG + chr3_4:72043627-72043646 Msa0519750:CDS 40.0%
AACAGAATCAGTGACTATGG+AGG + chr3_4:72043630-72043649 Msa0519750:CDS 40.0%
GTCAAAAGCAGCCTATTCAT+AGG + chr3_4:72043680-72043699 Msa0519750:CDS 40.0%
CAGAGAACATCTCAGTATAG+AGG + chr3_4:72043724-72043743 Msa0519750:CDS 40.0%
ATGGGATAACAGTTGCAAGA+AGG + chr3_4:72043963-72043982 Msa0519750:CDS 40.0%
GATAACAGTTGCAAGAAGGA+AGG + chr3_4:72043967-72043986 Msa0519750:CDS 40.0%
! CAAGCAGCTCTTAAGTATTG+GGG + chr3_4:72044314-72044333 Msa0519750:CDS 40.0%
GGGAAGTTTATGTGTGTTGA+AGG - chr3_4:72044341-72044360 None:intergenic 40.0%
CTAAGGAGATCAAATGTACC+GGG - chr3_4:72044361-72044380 None:intergenic 40.0%
GCTAAGGAGATCAAATGTAC+CGG - chr3_4:72044362-72044381 None:intergenic 40.0%
! GAAAAAGCAGTGGATTCTCA+AGG + chr3_4:72044626-72044645 Msa0519750:intron 40.0%
! AAAAGCAGTGGATTCTCAAG+GGG + chr3_4:72044628-72044647 Msa0519750:CDS 40.0%
! TTTTGCAGACATCACCAACA+TGG + chr3_4:72044758-72044777 Msa0519750:intron 40.0%
GGTAACAAGGACCTTTATCT+TGG + chr3_4:72044812-72044831 Msa0519750:CDS 40.0%
GTAGCAGCAATAAAATTCCG+TGG + chr3_4:72045225-72045244 Msa0519750:CDS 40.0%
TTGTAACAGCATTTGCTCCA+CGG - chr3_4:72045245-72045264 None:intergenic 40.0%
GCTAGCAATACTCTTCTTTC+TGG + chr3_4:72045303-72045322 Msa0519750:CDS 40.0%
TATTCATGAACTTCGGTCTG+TGG - chr3_4:72045360-72045379 None:intergenic 40.0%
CTGCAAAACCATCTTCCATT+TGG - chr3_4:72045460-72045479 None:intergenic 40.0%
ACTCTTTCCAAAGCCTTCAA+TGG + chr3_4:72045701-72045720 Msa0519750:CDS 40.0%
CACAAGTAGCTACACAAATG+AGG + chr3_4:72045769-72045788 Msa0519750:CDS 40.0%
CATTCCATGCAGCAAAAACT+GGG - chr3_4:72045824-72045843 None:intergenic 40.0%
TCATTCCATGCAGCAAAAAC+TGG - chr3_4:72045825-72045844 None:intergenic 40.0%
!! AAAGGGTTGGTTGGGAAATT+TGG + chr3_4:72045952-72045971 Msa0519750:three_prime_UTR 40.0%
TAGCCATGAAAGTGAGGCAA+TGG + chr3_4:72043183-72043202 Msa0519750:CDS 45.0%
AAGCCATTGCCTCACTTTCA+TGG - chr3_4:72043189-72043208 None:intergenic 45.0%
CTGAAAGAAGGTCAAGGGAA+TGG - chr3_4:72043276-72043295 None:intergenic 45.0%
GTTCCCAAATGCCTCAAAAC+TGG + chr3_4:72043413-72043432 Msa0519750:CDS 45.0%
GCAGATGAACACTACTAGCA+TGG + chr3_4:72043462-72043481 Msa0519750:CDS 45.0%
ACTAGCATGGGGAGTCATAA+TGG + chr3_4:72043475-72043494 Msa0519750:CDS 45.0%
GTTGTTCGTACTGTAGGAAG+TGG + chr3_4:72043523-72043542 Msa0519750:CDS 45.0%
TTAAGCCTTTCAATGAGCCC+TGG + chr3_4:72043559-72043578 Msa0519750:CDS 45.0%
CACAGCTAGATTGAGAACCA+GGG - chr3_4:72043579-72043598 None:intergenic 45.0%
ACACAGCTAGATTGAGAACC+AGG - chr3_4:72043580-72043599 None:intergenic 45.0%
!! GTCACTGATTCTGTTCCAGA+AGG - chr3_4:72043625-72043644 None:intergenic 45.0%
AAAAGAGGAGCTGCTAAGCT+TGG + chr3_4:72043658-72043677 Msa0519750:CDS 45.0%
!! CTCAGTATAGAGGTGTCACA+AGG + chr3_4:72043734-72043753 Msa0519750:CDS 45.0%
TCCATTAGGCATAGATGGAC+TGG + chr3_4:72043922-72043941 Msa0519750:intron 45.0%
ACCAGTCCATCTATGCCTAA+TGG - chr3_4:72043926-72043945 None:intergenic 45.0%
TGGAAGAGTTGCTGGTAACA+AGG + chr3_4:72044799-72044818 Msa0519750:CDS 45.0%
! TTCTTTCTGGTCAGCATGCA+AGG + chr3_4:72045316-72045335 Msa0519750:CDS 45.0%
AATGTATCGAGCCAGACCAA+TGG + chr3_4:72045393-72045412 Msa0519750:CDS 45.0%
!! AGTGAGGGAGCAGTTTTATC+AGG - chr3_4:72045681-72045700 None:intergenic 45.0%
!! GGCTTTGGAAAGAGTAGTGA+GGG - chr3_4:72045696-72045715 None:intergenic 45.0%
!! AGGCTTTGGAAAGAGTAGTG+AGG - chr3_4:72045697-72045716 None:intergenic 45.0%
! ACTTCCCAGTTTTTGCTGCA+TGG + chr3_4:72045817-72045836 Msa0519750:CDS 45.0%
GAAAGAAGCAGGAACCGTGT+TGG - chr3_4:72042750-72042769 None:intergenic 50.0%
AGCATGGGGAGTCATAATGG+TGG + chr3_4:72043478-72043497 Msa0519750:CDS 50.0%
! AGTCATAATGGTGGAGCTGG+TGG + chr3_4:72043487-72043506 Msa0519750:CDS 50.0%
GAGAACCAGGGCTCATTGAA+AGG - chr3_4:72043567-72043586 None:intergenic 50.0%
CCAGAAGGTGAGATCTGTCT+AGG - chr3_4:72043610-72043629 None:intergenic 50.0%
CCTAGACAGATCTCACCTTC+TGG + chr3_4:72043607-72043626 Msa0519750:CDS 50.0%
! AGGGGTGCATCAATGTACAG+AGG + chr3_4:72044646-72044665 Msa0519750:CDS 50.0%
! CAATGTACAGAGGCGTGACA+AGG + chr3_4:72044656-72044675 Msa0519750:CDS 50.0%
GCAGACATCACCAACATGGT+AGG + chr3_4:72044762-72044781 Msa0519750:intron 50.0%
GACATCACCAACATGGTAGG+TGG + chr3_4:72044765-72044784 Msa0519750:intron 50.0%
TTGCTTGCCACCTACCATGT+TGG - chr3_4:72044775-72044794 None:intergenic 50.0%
AACATGGTAGGTGGCAAGCA+AGG + chr3_4:72044774-72044793 Msa0519750:CDS 50.0%
GCAAGGATTGGAAGAGTTGC+TGG + chr3_4:72044791-72044810 Msa0519750:CDS 50.0%
!! GGGTTGGTTGGGAAATTTGG+AGG + chr3_4:72045955-72045974 Msa0519750:three_prime_UTR 50.0%
! GGGAGTCATAATGGTGGAGC+TGG + chr3_4:72043484-72043503 Msa0519750:CDS 55.0%
! CATAATGGTGGAGCTGGTGG+TGG + chr3_4:72043490-72043509 Msa0519750:CDS 55.0%
GGTAGGTGGCAAGCAAGGAT+TGG + chr3_4:72044779-72044798 Msa0519750:CDS 55.0%
CAGCAGCTTCTCCATTGGTC+TGG - chr3_4:72045407-72045426 None:intergenic 55.0%
CGCTTCAGCAGCTTCTCCAT+TGG - chr3_4:72045412-72045431 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 72042304 72046106 72042304 ID=Msa0519750;Name=Msa0519750
chr3_4 mRNA 72042304 72046106 72042304 ID=Msa0519750-mRNA-1;Parent=Msa0519750;Name=Msa0519750-mRNA-1;_AED=0.07;_eAED=0.07;_QI=301|0.85|0.75|1|1|1|8|255|665
chr3_4 exon 72042304 72042923 72042304 ID=Msa0519750-mRNA-1:exon:19801;Parent=Msa0519750-mRNA-1
chr3_4 exon 72043110 72043755 72043110 ID=Msa0519750-mRNA-1:exon:19802;Parent=Msa0519750-mRNA-1
chr3_4 exon 72043930 72044012 72043930 ID=Msa0519750-mRNA-1:exon:19803;Parent=Msa0519750-mRNA-1
chr3_4 exon 72044273 72044361 72044273 ID=Msa0519750-mRNA-1:exon:19804;Parent=Msa0519750-mRNA-1
chr3_4 exon 72044457 72044530 72044457 ID=Msa0519750-mRNA-1:exon:19805;Parent=Msa0519750-mRNA-1
chr3_4 exon 72044627 72044677 72044627 ID=Msa0519750-mRNA-1:exon:19806;Parent=Msa0519750-mRNA-1
chr3_4 exon 72044766 72044842 72044766 ID=Msa0519750-mRNA-1:exon:19807;Parent=Msa0519750-mRNA-1
chr3_4 exon 72045193 72046106 72045193 ID=Msa0519750-mRNA-1:exon:19808;Parent=Msa0519750-mRNA-1
chr3_4 five_prime_UTR 72042304 72042604 72042304 ID=Msa0519750-mRNA-1:five_prime_utr;Parent=Msa0519750-mRNA-1
chr3_4 CDS 72042605 72042923 72042605 ID=Msa0519750-mRNA-1:cds;Parent=Msa0519750-mRNA-1
chr3_4 CDS 72043110 72043755 72043110 ID=Msa0519750-mRNA-1:cds;Parent=Msa0519750-mRNA-1
chr3_4 CDS 72043930 72044012 72043930 ID=Msa0519750-mRNA-1:cds;Parent=Msa0519750-mRNA-1
chr3_4 CDS 72044273 72044361 72044273 ID=Msa0519750-mRNA-1:cds;Parent=Msa0519750-mRNA-1
chr3_4 CDS 72044457 72044530 72044457 ID=Msa0519750-mRNA-1:cds;Parent=Msa0519750-mRNA-1
chr3_4 CDS 72044627 72044677 72044627 ID=Msa0519750-mRNA-1:cds;Parent=Msa0519750-mRNA-1
chr3_4 CDS 72044766 72044842 72044766 ID=Msa0519750-mRNA-1:cds;Parent=Msa0519750-mRNA-1
chr3_4 CDS 72045193 72045851 72045193 ID=Msa0519750-mRNA-1:cds;Parent=Msa0519750-mRNA-1
chr3_4 three_prime_UTR 72045852 72046106 72045852 ID=Msa0519750-mRNA-1:three_prime_utr;Parent=Msa0519750-mRNA-1
Gene Sequence

>Msa0519750

ATGAAGTCCATGAATGACAGTAGTAACTCTGATGACAACAATCATAACAACAATTGGTTAGGTTTCTCTCTCTCACCCCACATTTCTACTTCTTCTCCTCATCATCATCATCATTACCAACAAACTCAAACTTCTTCAGTTTCCAACACGGTTCCTGCTTCTTTCTATTTTTCACCTTCTCACTTCACAAACTCAACAATCTGTTATGGTGTTCCTGAAAATGGTAACAACTTTCATTCACCTAATTTGACTGTTATGCCTATCAAGTCAGATGGATCACTTTGTATCATGGAAGCTCTTGGTAGATCACAATCACAAGTGATGGTGCCATCTTCATCTCCTAAACTAGAGGATTTTCTTGGTGGTGCAACAATGGGTAGTGATGAATATGGTAGCCATGAAAGTGAGGCAATGGCTTTAAGCTTAGACAGCATCTACTATAATAACCAACAAAATGCAGATCCTCATCAAGCTAATACAGACCATTCCCTTGACCTTCTTTCAGAATCTTTCAGACAACAAACATCACATCCATATTATGCAGCACTTGGTTATCATGGCCTATTTCAAGCACCATTAGAGGTAGAATCTAAAGAAAACATAAACCATGTTGATGTTTCTAGTTCCCAAATGCCTCAAAACTGGTATTCTGCTTCTCAAGCTTTAGAGCAGCAGATGAACACTACTAGCATGGGGAGTCATAATGGTGGAGCTGGTGGTGGTTCTTCTTCTGTTGTTCGTACTGTAGGAAGTGGCGAGTTACAATCTTTAAGCCTTTCAATGAGCCCTGGTTCTCAATCTAGCTGTGTTACTGTTCCTAGACAGATCTCACCTTCTGGAACAGAATCAGTGACTATGGAGGCTAAAAAAAGAGGAGCTGCTAAGCTTGGTCAAAAGCAGCCTATTCATAGGAAATCTATTGACACTTTTGGACAGAGAACATCTCAGTATAGAGGTGTCACAAGGCATAGATGGACTGGTAGATATGAAGCACATTTATGGGATAACAGTTGCAAGAAGGAAGGACAAACAAGAAAAGGAAGACAAGGTGGTTATGATATGGAAGAGAAAGCTGCAAGAGCTTATGATCAAGCAGCTCTTAAGTATTGGGGACCTTCAACACACATAAACTTCCCGCTAGAAAACTACCATACTCAACTTGAGGAAATGAAGAATATGACAAGGCAGGAATATGTTGCACATTTGAGAAGAAAAAGCAGTGGATTCTCAAGGGGTGCATCAATGTACAGAGGCGTGACAAGACATCACCAACATGGTAGGTGGCAAGCAAGGATTGGAAGAGTTGCTGGTAACAAGGACCTTTATCTTGGAACATTCAGCACTCAAGAGGAAGCAGCTGAAGCATATGATGTAGCAGCAATAAAATTCCGTGGAGCAAATGCTGTTACAAATTTTGACATATCTAAATATGATGTTGAAAGGATCATGGCTAGCAATACTCTTCTTTCTGGTCAGCATGCAAGGAGAATCAAAGATAAGGATCCACAGACCGAAGTTCATGAATATAATCATAGTACTAATGTATCGAGCCAGACCAATGGAGAAGCTGCTGAAGCGCAAAACGTTAACGAAAATAATGATTCCAAATGGAAGATGGTTTTGCAGCAACAGCAATCAAATAGTTGTGATAAAAAAATCATTGTGAATTCTGATGGAAGTTACAAGAATTCTGATTTCTCTATGTCTTTACAAGACCTTGTTGGTATTAATTCAGTTGGTTTGGATGATTCTACCAAAATAGGGACACATTTTTCAAATCCATCATCATTGGTGACAAGTTTAAGCAGTTCAAGAGAAGCTAGTCCTGATAAAACTGCTCCCTCACTACTCTTTCCAAAGCCTTCAATGGAATCAAAAATTGCCACTAATGTTGCTGTTAGTTCTTGGTTTCCTTCACAAGTAGCTACACAAATGAGGCCAGCATCTTCAATCAATTTGTCTCACTTCCCAGTTTTTGCTGCATGGAATGAGACCTAG
Protein sequence

>Msa0519750

MKSMNDSSNSDDNNHNNNWLGFSLSPHISTSSPHHHHHYQQTQTSSVSNTVPASFYFSPSHFTNSTICYGVPENGNNFHSPNLTVMPIKSDGSLCIMEALGRSQSQVMVPSSSPKLEDFLGGATMGSDEYGSHESEAMALSLDSIYYNNQQNADPHQANTDHSLDLLSESFRQQTSHPYYAALGYHGLFQAPLEVESKENINHVDVSSSQMPQNWYSASQALEQQMNTTSMGSHNGGAGGGSSSVVRTVGSGELQSLSLSMSPGSQSSCVTVPRQISPSGTESVTMEAKKRGAAKLGQKQPIHRKSIDTFGQRTSQYRGVTRHRWTGRYEAHLWDNSCKKEGQTRKGRQGGYDMEEKAARAYDQAALKYWGPSTHINFPLENYHTQLEEMKNMTRQEYVAHLRRKSSGFSRGASMYRGVTRHHQHGRWQARIGRVAGNKDLYLGTFSTQEEAAEAYDVAAIKFRGANAVTNFDISKYDVERIMASNTLLSGQHARRIKDKDPQTEVHEYNHSTNVSSQTNGEAAEAQNVNENNDSKWKMVLQQQQSNSCDKKIIVNSDGSYKNSDFSMSLQDLVGINSVGLDDSTKIGTHFSNPSSLVTSLSSSREASPDKTAPSLLFPKPSMESKIATNVAVSSWFPSQVATQMRPASSINLSHFPVFAAWNET*