AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1427100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1427100 MtrunA17_Chr3g0135161 94.162 668 26 5 1 665 1 658 0.0 1245
Msa1427100 MtrunA17_Chr1g0152161 60.197 711 184 23 1 664 1 659 0.0 733
Msa1427100 MtrunA17_Chr4g0054351 51.879 692 270 19 1 664 1 657 0.0 544
Msa1427100 MtrunA17_Chr8g0343851 47.984 496 187 11 18 502 3 438 2.03e-137 414
Msa1427100 MtrunA17_Chr5g0400871 46.394 513 187 12 1 501 4 440 5.53e-126 384
Msa1427100 MtrunA17_Chr7g0249621 77.358 212 44 2 302 510 248 458 1.21e-110 348
Msa1427100 MtrunA17_Chr4g0032631 66.800 250 61 4 254 499 122 353 2.57e-110 343
Msa1427100 MtrunA17_Chr4g0072291 62.313 268 86 3 239 491 73 340 3.16e-110 342
Msa1427100 MtrunA17_Chr2g0328971 65.182 247 64 2 254 499 129 354 3.52e-109 339
Msa1427100 MtrunA17_Chr5g0412151 76.768 198 42 2 304 498 215 411 1.47e-95 304
Msa1427100 MtrunA17_Chr4g0073521 71.084 166 48 0 312 477 46 211 9.85e-82 262
Msa1427100 MtrunA17_Chr7g0216601 62.234 188 67 2 293 477 46 232 3.51e-75 246
Msa1427100 MtrunA17_Chr8g0355191 59.512 205 76 2 280 477 15 219 5.94e-75 246
Msa1427100 MtrunA17_Chr6g0452911 58.411 214 71 3 274 477 30 235 5.60e-74 243
Msa1427100 MtrunA17_Chr8g0367311 63.842 177 57 2 350 520 22 197 2.82e-71 231
Msa1427100 MtrunA17_Chr4g0002011 68.047 169 51 1 312 477 35 203 1.95e-70 232
Msa1427100 MtrunA17_Chr8g0355181 59.487 195 70 2 312 506 55 240 1.36e-69 231
Msa1427100 MtrunA17_Chr8g0362541 60.104 193 63 1 299 477 55 247 1.43e-68 229
Msa1427100 MtrunA17_Chr2g0305821 65.574 122 40 1 304 423 36 157 4.48e-44 155
Msa1427100 MtrunA17_Chr4g0053181 53.939 165 68 3 316 480 176 332 1.28e-41 158
Msa1427100 MtrunA17_Chr5g0401891 53.939 165 68 3 316 480 152 308 4.56e-41 157
Msa1427100 MtrunA17_Chr7g0257361 57.237 152 54 3 289 437 36 179 1.27e-40 153
Msa1427100 MtrunA17_Chr7g0263581 50.909 165 73 3 316 480 139 295 1.96e-39 151
Msa1427100 MtrunA17_Chr4g0030191 50.909 165 73 3 316 480 131 287 4.65e-39 150
Msa1427100 MtrunA17_Chr2g0325911 41.410 227 94 8 270 480 72 275 5.72e-32 129
Msa1427100 MtrunA17_Chr2g0305831 43.165 139 62 4 419 542 1 137 6.40e-25 103
Msa1427100 MtrunA17_Chr3g0126151 47.368 95 49 1 361 455 47 140 2.84e-22 93.2
Msa1427100 MtrunA17_Chr8g0367321 85.366 41 6 0 311 351 153 193 1.52e-19 87.4
Msa1427100 MtrunA17_Chr1g0170761 40.426 141 76 3 292 432 113 245 4.09e-17 84.0
Msa1427100 MtrunA17_Chr1g0158971 53.030 66 27 2 407 472 109 170 2.22e-11 65.9
Msa1427100 MtrunA17_Chr8g0375031 53.125 64 29 1 416 479 125 187 2.60e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1427100 AT4G37750.1 52.158 533 151 15 1 496 1 466 2.69e-155 460
Msa1427100 AT1G72570.1 70.370 243 67 3 257 495 164 405 1.97e-117 357
Msa1427100 AT1G51190.1 67.460 252 73 4 254 499 129 377 4.32e-113 352
Msa1427100 AT3G20840.1 68.000 250 69 5 254 499 126 368 7.20e-111 346
Msa1427100 AT5G57390.2 77.387 199 42 1 296 491 65 263 1.43e-110 340
Msa1427100 AT5G57390.1 77.387 199 42 1 296 491 183 381 1.27e-109 342
Msa1427100 AT5G17430.2 67.755 245 74 2 304 545 136 378 3.27e-107 335
Msa1427100 AT5G17430.1 67.755 245 74 2 304 545 198 440 8.47e-107 336
Msa1427100 AT5G10510.2 75.897 195 46 1 304 498 218 411 1.90e-92 297
Msa1427100 AT5G65510.4 67.907 215 66 1 289 500 146 360 1.06e-90 289
Msa1427100 AT5G65510.2 67.907 215 66 1 289 500 204 418 1.32e-90 291
Msa1427100 AT5G65510.1 67.907 215 66 1 289 500 204 418 1.32e-90 291
Msa1427100 AT5G10510.7 74.747 198 46 2 304 498 218 414 2.08e-90 291
Msa1427100 AT5G10510.5 74.747 198 46 2 304 498 218 414 2.08e-90 291
Msa1427100 AT5G10510.1 74.747 198 46 2 304 498 241 437 3.19e-90 292
Msa1427100 AT5G10510.6 74.747 198 46 2 304 498 256 452 4.02e-90 292
Msa1427100 AT5G10510.4 74.747 198 46 2 304 498 256 452 4.02e-90 292
Msa1427100 AT5G10510.3 67.431 218 47 2 304 498 256 472 6.32e-86 281
Msa1427100 AT5G65510.3 70.807 161 44 1 289 446 146 306 1.10e-77 251
Msa1427100 AT5G65510.3 42.667 75 35 1 414 480 172 246 5.08e-11 64.7
Msa1427100 AT3G54320.3 67.241 174 53 2 312 482 61 233 5.48e-69 231
Msa1427100 AT3G54320.1 67.241 174 53 2 312 482 61 233 8.60e-69 231
Msa1427100 AT1G79700.2 68.047 169 51 1 312 477 48 216 2.47e-63 213
Msa1427100 AT1G16060.1 58.373 209 84 1 272 477 14 222 2.63e-62 211
Msa1427100 AT1G79700.1 63.905 169 48 2 312 477 48 206 1.47e-55 191
Msa1427100 AT3G54320.4 69.173 133 40 1 350 482 36 167 1.33e-51 183
Msa1427100 AT3G54320.5 69.173 133 40 1 350 482 36 167 1.33e-51 183
Msa1427100 AT1G79700.3 67.969 128 41 0 350 477 13 140 9.12e-44 157
Msa1427100 AT1G79700.4 67.969 128 41 0 350 477 7 134 9.40e-44 157
Msa1427100 AT4G36920.1 49.500 200 92 4 281 480 97 287 3.14e-42 159
Msa1427100 AT4G36920.2 49.500 200 92 4 281 480 97 287 3.14e-42 159
Msa1427100 AT1G16060.3 67.969 128 41 0 350 477 25 152 4.72e-42 154
Msa1427100 AT5G67180.2 43.534 232 113 4 260 491 50 263 9.25e-42 154
Msa1427100 AT5G67180.3 43.043 230 113 4 260 489 50 261 4.97e-41 151
Msa1427100 AT5G67180.1 43.534 232 113 4 260 491 50 263 5.03e-41 153
Msa1427100 AT2G41710.1 56.579 152 55 3 289 437 46 189 1.64e-40 154
Msa1427100 AT2G41710.4 56.579 152 55 3 289 437 46 189 3.02e-40 154
Msa1427100 AT2G41710.2 54.777 157 55 4 289 437 46 194 4.05e-38 147
Msa1427100 AT2G28550.3 50.303 165 74 3 316 480 153 309 4.99e-38 147
Msa1427100 AT2G41710.3 45.989 187 55 4 289 437 46 224 4.99e-34 135
Msa1427100 AT3G54320.2 70.968 93 26 1 390 482 1 92 7.49e-33 128
Msa1427100 AT1G16060.2 60.909 110 38 1 390 494 1 110 1.00e-31 123
Msa1427100 AT1G16060.4 60.909 110 38 1 390 494 1 110 1.00e-31 123
Msa1427100 AT1G16060.6 60.909 110 38 1 390 494 1 110 1.00e-31 123
Msa1427100 AT1G16060.5 60.909 110 38 1 390 494 1 110 1.00e-31 123
Msa1427100 AT2G28550.4 49.275 138 62 3 316 453 153 282 1.26e-30 123
Msa1427100 AT5G67180.4 39.013 223 117 5 260 482 50 253 2.31e-30 120
Msa1427100 AT2G28550.1 46.061 165 66 4 316 480 153 294 5.30e-30 124
Msa1427100 AT2G28550.2 49.194 124 55 3 316 439 153 268 3.87e-26 108
Msa1427100 AT2G28550.6 49.194 124 55 3 316 439 153 268 3.87e-26 108
Msa1427100 AT2G39250.2 42.202 109 56 2 317 425 109 210 1.31e-22 97.8
Msa1427100 AT2G39250.1 42.202 109 56 2 317 425 109 210 3.77e-22 98.6
Msa1427100 AT2G39250.1 29.368 269 150 12 395 656 88 323 5.90e-11 64.7
Msa1427100 AT2G28550.5 45.802 131 55 2 350 480 14 128 9.73e-22 96.3
Msa1427100 AT5G60120.6 39.181 171 80 6 300 470 145 291 2.13e-19 92.4
Msa1427100 AT5G60120.1 39.181 171 80 6 300 470 145 291 2.24e-19 92.4
Msa1427100 AT5G60120.4 45.238 126 61 3 313 438 157 274 2.33e-19 91.3
Msa1427100 AT5G60120.3 45.238 126 61 3 313 438 157 274 2.33e-19 91.3
Msa1427100 AT5G60120.5 45.238 126 61 3 313 438 157 274 2.33e-19 91.3
Msa1427100 AT5G60120.2 39.181 171 80 6 300 470 145 291 3.56e-19 91.7
Msa1427100 AT3G54990.2 46.154 104 49 2 316 419 122 218 1.57e-17 83.2
Msa1427100 AT3G54990.1 46.154 104 49 2 316 419 122 218 5.23e-17 83.6
Msa1427100 AT1G16060.7 62.025 79 25 1 421 494 10 88 6.45e-17 79.7

Find 145 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGGGTTGGTTGGGAAATT+TGG 0.137392 tig0026160:+40613 Msa1427100:three_prime_UTR
TTGGATGATTCTACCAAAAT+AGG 0.146027 tig0026160:+40255 Msa1427100:CDS
TGAATGAATGTTAGGACTTT+TGG 0.149737 tig0026160:+40644 Msa1427100:three_prime_UTR
GCTAGCAATACTCTTCTTTC+TGG 0.154896 tig0026160:+39964 Msa1427100:CDS
TATCTTTCTTTACTTGAAAA+AGG 0.199835 tig0026160:-37227 None:intergenic
CTAATGTTGCTGTTAGTTCT+TGG 0.205616 tig0026160:+40400 Msa1427100:CDS
AGGAAATCTATTGACACTTT+TGG 0.225909 tig0026160:+38359 Msa1427100:CDS
GTAGATATGAAGCACATTTA+TGG 0.228782 tig0026160:+38603 Msa1427100:CDS
CCTCTAATGGTGCTTGAAAT+AGG 0.284929 tig0026160:-38010 None:intergenic
CTGAAGAAGTTTGAGTTTGT+TGG 0.307916 tig0026160:-37379 None:intergenic
AAGCCATTGCCTCACTTTCA+TGG 0.308137 tig0026160:-37845 None:intergenic
CTGCAAAACCATCTTCCATT+TGG 0.319566 tig0026160:-40118 None:intergenic
TGTTATGGTGTTCCTGAAAA+TGG 0.323560 tig0026160:+37464 Msa1427100:CDS
AACTCAAGGTGGTTATGATA+TGG 0.323727 tig0026160:+38926 Msa1427100:intron
TCTTTAGATTCTACCTCTAA+TGG 0.324936 tig0026160:-38023 None:intergenic
CCAAGAAAATCCTCTAGTTT+AGG 0.328835 tig0026160:-37789 None:intergenic
TTTGATTCCATTGAAGGCTT+TGG 0.329887 tig0026160:-40369 None:intergenic
TATTCTCCATTAGGCATAGA+TGG 0.336451 tig0026160:+38576 Msa1427100:intron
TAACCAAGTGCTGCATAATA+TGG 0.342134 tig0026160:-37981 None:intergenic
TCATTTCCTCAAGTTGAGTA+TGG 0.344608 tig0026160:-39129 None:intergenic
ATAGGCATAACAGTCAAATT+AGG 0.347664 tig0026160:-37503 None:intergenic
TCAAGCAGCTCTTAAGTATT+GGG 0.347974 tig0026160:+38974 Msa1427100:CDS
TATATATATGTTTGTTTGGT+TGG 0.349105 tig0026160:+37013 Msa1427100:five_prime_UTR
AGGATTAATGAATGAATGTT+AGG 0.358146 tig0026160:+40636 Msa1427100:three_prime_UTR
CCTATTTCAAGCACCATTAG+AGG 0.368785 tig0026160:+38010 Msa1427100:CDS
CCTAGACAGATCTCACCTTC+TGG 0.379310 tig0026160:+38266 Msa1427100:CDS
CCTTAACAAACTTAGCCTTT+TGG 0.380384 tig0026160:+40708 Msa1427100:three_prime_UTR
CGCTTCAGCAGCTTCTCCAT+TGG 0.384097 tig0026160:-40070 None:intergenic
AGAGTTACTACTGTCATTCA+TGG 0.391896 tig0026160:-37270 None:intergenic
CAGCAGCTTCTCCATTGGTC+TGG 0.395700 tig0026160:-40065 None:intergenic
GGTAACAAGGACCTTTATCT+TGG 0.399524 tig0026160:+39473 Msa1427100:CDS
GTTCCCAAATGCCTCAAAAC+TGG 0.400777 tig0026160:+38072 Msa1427100:CDS
AATCATAACAACAATTGGTT+AGG 0.403766 tig0026160:+37302 Msa1427100:CDS
TAGATATGAAGCACATTTAT+GGG 0.403876 tig0026160:+38604 Msa1427100:CDS
TGGTATTAATTCAGTTGGTT+TGG 0.404298 tig0026160:+40236 Msa1427100:CDS
GAAGAGAAATAGATTCTGTT+AGG 0.405652 tig0026160:+37187 Msa1427100:five_prime_UTR
GCAAGGATTGGAAGAGTTGC+TGG 0.408354 tig0026160:+39452 Msa1427100:CDS
ACTCTTTCCAAAGCCTTCAA+TGG 0.408843 tig0026160:+40362 Msa1427100:CDS
TGGAACAGAATCAGTGACTA+TGG 0.411439 tig0026160:+38286 Msa1427100:CDS
ACAACAATCATAACAACAAT+TGG 0.411645 tig0026160:+37297 Msa1427100:CDS
TACTGAATGTTCCAAGATAA+AGG 0.412580 tig0026160:-39484 None:intergenic
AGTTTATATATATGTTTGTT+TGG 0.414467 tig0026160:+37009 Msa1427100:five_prime_UTR
TGGATGATTCTACCAAAATA+GGG 0.417120 tig0026160:+40256 Msa1427100:CDS
AAGGAAGGACAAACAAGAAA+AGG 0.424488 tig0026160:+38641 Msa1427100:CDS
TTCAACACACATAAACTTCC+CGG 0.425153 tig0026160:+39001 Msa1427100:CDS
TATGCAGCACTTGGTTATCA+TGG 0.425353 tig0026160:+37987 Msa1427100:CDS
CCAAAAGGCTAAGTTTGTTA+AGG 0.429179 tig0026160:-40708 None:intergenic
ACTAGCATGGGGAGTCATAA+TGG 0.434049 tig0026160:+38134 Msa1427100:CDS
AGGAGAAGAAGTAGAAATGT+GGG 0.436963 tig0026160:-37339 None:intergenic
TCAATAGATTTCCTATGAAT+AGG 0.438789 tig0026160:-38350 None:intergenic
ACAAACTCAACAATCTGTTA+TGG 0.440794 tig0026160:+37449 Msa1427100:CDS
ATGAATGAGAATATGCATAT+TGG 0.443517 tig0026160:+40743 Msa1427100:three_prime_UTR
ATCAAGCAGCTCTTAAGTAT+TGG 0.446724 tig0026160:+38973 Msa1427100:CDS
TCTTCTGTTGTTCGTACTGT+AGG 0.447306 tig0026160:+38176 Msa1427100:CDS
TTCTTTCTGGTCAGCATGCA+AGG 0.453728 tig0026160:+39977 Msa1427100:CDS
AGTGATCCATCTGACTTGAT+AGG 0.454921 tig0026160:-37521 None:intergenic
AAACTTGTCACCAATGATGA+TGG 0.469910 tig0026160:-40294 None:intergenic
TAATGATTCCAAATGGAAGA+TGG 0.471281 tig0026160:+40110 Msa1427100:CDS
ATGTCTTTACAAGACCTTGT+TGG 0.474882 tig0026160:+40216 Msa1427100:CDS
CTTTGTATCATGGAAGCTCT+TGG 0.479184 tig0026160:+37542 Msa1427100:CDS
AAAATCATTGTGAATTCTGA+TGG 0.483714 tig0026160:+40168 Msa1427100:CDS
GGGAAGTTTATGTGTGTTGA+AGG 0.488674 tig0026160:-38999 None:intergenic
CTTGTTGGTATTAATTCAGT+TGG 0.489454 tig0026160:+40231 Msa1427100:CDS
AGAACTAACAGCAACATTAG+TGG 0.490687 tig0026160:-40397 None:intergenic
ACGAAAATAATGATTCCAAA+TGG 0.491698 tig0026160:+40103 Msa1427100:CDS
GTCAAAAGCAGCCTATTCAT+AGG 0.492472 tig0026160:+38339 Msa1427100:CDS
GGTAGGTGGCAAGCAAGGAT+TGG 0.507927 tig0026160:+39440 Msa1427100:CDS
CATCCATATTATGCAGCACT+TGG 0.508376 tig0026160:+37978 Msa1427100:CDS
TTTCTTGGTGGTGCAACAAT+GGG 0.508995 tig0026160:+37804 Msa1427100:CDS
GGGTTGGTTGGGAAATTTGG+AGG 0.511636 tig0026160:+40616 Msa1427100:three_prime_UTR
AGATGGATCACTTTGTATCA+TGG 0.512780 tig0026160:+37532 Msa1427100:CDS
ACACAGCTAGATTGAGAACC+AGG 0.513570 tig0026160:-38236 None:intergenic
GTTGTTCGTACTGTAGGAAG+TGG 0.513611 tig0026160:+38182 Msa1427100:CDS
AGTCATAATGGTGGAGCTGG+TGG 0.513615 tig0026160:+38146 Msa1427100:CDS
TCCATTAGGCATAGATGGAC+TGG 0.517509 tig0026160:+38581 Msa1427100:intron
ATATGATGTTGAAAGGATCA+TGG 0.520797 tig0026160:+39942 Msa1427100:CDS
AATGAGAATATGCATATTGG+AGG 0.520951 tig0026160:+40746 Msa1427100:three_prime_UTR
CATAATGGTGGAGCTGGTGG+TGG 0.521042 tig0026160:+38149 Msa1427100:CDS
GAGGAGAAGAAGTAGAAATG+TGG 0.523031 tig0026160:-37340 None:intergenic
GGGAGTCATAATGGTGGAGC+TGG 0.523283 tig0026160:+38143 Msa1427100:CDS
CAAGGAGATATAATCTCTCT+AGG 0.523503 tig0026160:-40508 None:intergenic
GTCTGAAAGATTCTGAAAGA+AGG 0.527254 tig0026160:-37944 None:intergenic
GGCTTTGGAAAGAGTAGTGA+GGG 0.530598 tig0026160:-40354 None:intergenic
AAAAGAGGAGCTGCTAAGCT+TGG 0.531048 tig0026160:+38317 Msa1427100:CDS
AAGCTGAAGCAGAAGAATAG+AGG 0.531685 tig0026160:+37084 Msa1427100:five_prime_UTR
GAGAACCAGGGCTCATTGAA+AGG 0.533035 tig0026160:-38223 None:intergenic
CCAGAAGGTGAGATCTGTCT+AGG 0.534044 tig0026160:-38266 None:intergenic
TATCTAAATATGATGTTGAA+AGG 0.534738 tig0026160:+39935 Msa1427100:CDS
CATTCCAACATATAGCCAAA+AGG 0.534863 tig0026160:-40723 None:intergenic
TATTCATGAACTTCGGTCTG+TGG 0.536183 tig0026160:-40018 None:intergenic
GTTGAGTTTGTGAAGTGAGA+AGG 0.536370 tig0026160:-37437 None:intergenic
GACAAATTGATTGAAGATGC+TGG 0.538955 tig0026160:-40453 None:intergenic
TTAAGCCTTTCAATGAGCCC+TGG 0.541546 tig0026160:+38218 Msa1427100:CDS
CTGAAAGAAGGTCAAGGGAA+TGG 0.549375 tig0026160:-37932 None:intergenic
ATGGGATAACAGTTGCAAGA+AGG 0.549719 tig0026160:+38622 Msa1427100:CDS
ACAATGGGTAGTGATGAATA+TGG 0.550458 tig0026160:+37819 Msa1427100:CDS
CAGAGAACATCTCAGTATAG+AGG 0.550832 tig0026160:+38383 Msa1427100:CDS
TGCAAGGAGAATCAAAGATA+AGG 0.550976 tig0026160:+39993 Msa1427100:CDS
ATTTGTGTAGCTACTTGTGA+AGG 0.555126 tig0026160:-40426 None:intergenic
TTGCTTGCCACCTACCATGT+TGG 0.556430 tig0026160:-39433 None:intergenic
ATGATTATATTCATGAACTT+CGG 0.556488 tig0026160:-40025 None:intergenic
AATGAAGAATATGACAAGGC+AGG 0.556697 tig0026160:+39147 Msa1427100:CDS
GAAAGAAGCAGGAACAGTGT+TGG 0.556882 tig0026160:-37405 None:intergenic
CAGATGAACACTACTAGCAT+GGG 0.557648 tig0026160:+38122 Msa1427100:CDS
TGGAAGAGTTGCTGGTAACA+AGG 0.559839 tig0026160:+39460 Msa1427100:CDS
GCAGATGAACACTACTAGCA+TGG 0.560674 tig0026160:+38121 Msa1427100:CDS
TTGTAACAGCATTTGCTCCA+CGG 0.565794 tig0026160:-39903 None:intergenic
AGGCTTTGGAAAGAGTAGTG+AGG 0.568883 tig0026160:-40355 None:intergenic
AAGATTCTGAAAGAAGGTCA+AGG 0.578912 tig0026160:-37938 None:intergenic
CACAAGTAGCTACACAAATG+AGG 0.580768 tig0026160:+40430 Msa1427100:CDS
GTTATGCCTATCAAGTCAGA+TGG 0.582455 tig0026160:+37515 Msa1427100:CDS
AATATGTTGCACATTTGAGA+AGG 0.587308 tig0026160:+39170 Msa1427100:CDS
TAGCCATGAAAGTGAGGCAA+TGG 0.587506 tig0026160:+37842 Msa1427100:CDS
AAACTACCATACTCAACTTG+AGG 0.599024 tig0026160:+39123 Msa1427100:CDS
ATCTTCATCTCCTAAACTAG+AGG 0.599319 tig0026160:+37779 Msa1427100:CDS
AACATGGTAGGTGGCAAGCA+AGG 0.600209 tig0026160:+39435 Msa1427100:CDS
CAATGTACAGAGGCGTGACA+AGG 0.600518 tig0026160:+39317 Msa1427100:CDS
GTCACTGATTCTGTTCCAGA+AGG 0.600695 tig0026160:-38281 None:intergenic
GCAGACATCACCAACATGGT+AGG 0.604873 tig0026160:+39423 Msa1427100:intron
ATGTAACACAAGGGTAACAT+TGG 0.605982 tig0026160:+40685 Msa1427100:three_prime_UTR
AGATTCTGAAAGAAGGTCAA+GGG 0.611616 tig0026160:-37937 None:intergenic
CTCAGTATAGAGGTGTCACA+AGG 0.617014 tig0026160:+38393 Msa1427100:CDS
CAAGCAGCTCTTAAGTATTG+GGG 0.623947 tig0026160:+38975 Msa1427100:CDS
CAAACAAGAAAAGGAAGACA+AGG 0.624646 tig0026160:+38650 Msa1427100:CDS
AATGTATCGAGCCAGACCAA+TGG 0.631591 tig0026160:+40054 Msa1427100:CDS
TCTAGTTTAGGAGATGAAGA+TGG 0.633764 tig0026160:-37777 None:intergenic
GACATCACCAACATGGTAGG+TGG 0.639099 tig0026160:+39426 Msa1427100:intron
ATGAGAATATGCATATTGGA+GGG 0.640086 tig0026160:+40747 Msa1427100:three_prime_UTR
TGGTCTGTATTAGCTTGATG+AGG 0.640420 tig0026160:-37912 None:intergenic
GATAACAGTTGCAAGAAGGA+AGG 0.640507 tig0026160:+38626 Msa1427100:CDS
AAAAGCAGTGGATTCTCAAG+GGG 0.641907 tig0026160:+39289 Msa1427100:CDS
ATATGGTAGCCATGAAAGTG+AGG 0.643799 tig0026160:+37836 Msa1427100:CDS
CTTGGTAGATCACAATCACA+AGG 0.655160 tig0026160:+37560 Msa1427100:CDS
TTTGTCATAACATAGTAACA+AGG 0.658655 tig0026160:-40526 None:intergenic
GTTACATAAAGAAAACAAAG+TGG 0.660708 tig0026160:-40669 None:intergenic
CAACTGAATTAATACCAACA+AGG 0.665562 tig0026160:-40230 None:intergenic
GTAGCAGCAATAAAATTCCG+TGG 0.666673 tig0026160:+39886 Msa1427100:CDS
GGAGAAGAAGTAGAAATGTG+GGG 0.675273 tig0026160:-37338 None:intergenic
AACAGAATCAGTGACTATGG+AGG 0.680741 tig0026160:+38289 Msa1427100:CDS
AGCATGGGGAGTCATAATGG+TGG 0.689715 tig0026160:+38137 Msa1427100:CDS
AGGAAATGAAGAATATGACA+AGG 0.693051 tig0026160:+39143 Msa1427100:CDS
GGGAACTAGAAACATCAACA+TGG 0.697253 tig0026160:-38055 None:intergenic
CACAGCTAGATTGAGAACCA+GGG 0.711589 tig0026160:-38235 None:intergenic
AGATGAACACTACTAGCATG+GGG 0.712889 tig0026160:+38123 Msa1427100:CDS
AGGGGTGCATCAATGTACAG+AGG 0.720794 tig0026160:+39307 Msa1427100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAATAACAACTTTTTTT+TGG - tig0026160:37674-37693 None:intergenic 10.0%
!! AAAGAAAAAAAATTACAAAA+AGG - tig0026160:39815-39834 None:intergenic 10.0%
!!! GGATATTTATTTTTTAAAAA+GGG + tig0026160:40596-40615 Msa1427100:three_prime_UTR 10.0%
!! AGTTTATATATATGTTTGTT+TGG + tig0026160:37009-37028 Msa1427100:five_prime_UTR 15.0%
!! TCTTTAATTAATGTTAGATA+TGG + tig0026160:37696-37715 Msa1427100:intron 15.0%
!!! TGTTAGTATAAAATTTTAGT+AGG - tig0026160:38721-38740 None:intergenic 15.0%
!! ACAAATTCAAATGAAAAAAA+GGG - tig0026160:39361-39380 None:intergenic 15.0%
!!! TTGTAATTTTTTTTCTTTCT+TGG + tig0026160:39817-39836 Msa1427100:intron 15.0%
!! TATGTTATGACAAAAAAAAA+GGG + tig0026160:40535-40554 Msa1427100:three_prime_UTR 15.0%
!! ATTATGTATTGAAAAACATA+TGG + tig0026160:40573-40592 Msa1427100:three_prime_UTR 15.0%
!! TTATGTATTGAAAAACATAT+GGG + tig0026160:40574-40593 Msa1427100:three_prime_UTR 15.0%
!!! GGGATATTTATTTTTTAAAA+AGG + tig0026160:40595-40614 Msa1427100:three_prime_UTR 15.0%
!!! ATTTATTTTTTAAAAAGGGT+TGG + tig0026160:40600-40619 Msa1427100:three_prime_UTR 15.0%
!! TATATATATGTTTGTTTGGT+TGG + tig0026160:37013-37032 Msa1427100:five_prime_UTR 20.0%
!! TATCTTTCTTTACTTGAAAA+AGG - tig0026160:37230-37249 None:intergenic 20.0%
!!! AAATAACAACTTTTTTTTGG+TGG - tig0026160:37671-37690 None:intergenic 20.0%
!! AAAACAACATAATCATTACA+GGG - tig0026160:37722-37741 None:intergenic 20.0%
!! TTATTATATGATTGAAGTGA+TGG + tig0026160:37752-37771 Msa1427100:intron 20.0%
!!! TGTTTGTTTTTTCTTTTACT+TGG - tig0026160:38838-38857 None:intergenic 20.0%
!!! GCAATTATTTTGTAGTAAAA+AGG + tig0026160:39212-39231 Msa1427100:intron 20.0%
!! TAATTATATGACAATCATGA+TGG + tig0026160:39241-39260 Msa1427100:intron 20.0%
!! GACAAATTCAAATGAAAAAA+AGG - tig0026160:39362-39381 None:intergenic 20.0%
!!! TTTAAAGTAAAATCCTTCAT+AGG + tig0026160:39610-39629 Msa1427100:intron 20.0%
!! AGTAAATTAAATTCCTATGA+AGG - tig0026160:39626-39645 None:intergenic 20.0%
!!! TTTCTTGGAAATGAAATTTT+AGG + tig0026160:39832-39851 Msa1427100:intron 20.0%
!! TATCTAAATATGATGTTGAA+AGG + tig0026160:39935-39954 Msa1427100:CDS 20.0%
!! ATGATTATATTCATGAACTT+CGG - tig0026160:40028-40047 None:intergenic 20.0%
!! CTATGTTATGACAAAAAAAA+AGG + tig0026160:40534-40553 Msa1427100:three_prime_UTR 20.0%
!! ACAAAAAAAAAGGGTAAAAA+GGG + tig0026160:40544-40563 Msa1427100:three_prime_UTR 20.0%
!! TATGTATTGAAAAACATATG+GGG + tig0026160:40575-40594 Msa1427100:three_prime_UTR 20.0%
! ACAACAATCATAACAACAAT+TGG + tig0026160:37297-37316 Msa1427100:CDS 25.0%
! AATCATAACAACAATTGGTT+AGG + tig0026160:37302-37321 Msa1427100:CDS 25.0%
! GAAAACAACATAATCATTAC+AGG - tig0026160:37723-37742 None:intergenic 25.0%
! TCAATAGATTTCCTATGAAT+AGG - tig0026160:38353-38372 None:intergenic 25.0%
!!! GATTTTGTTTATTCTCCATT+AGG + tig0026160:38567-38586 Msa1427100:intron 25.0%
! TAGATATGAAGCACATTTAT+GGG + tig0026160:38604-38623 Msa1427100:CDS 25.0%
! ATGCATGTTACAGTATATTT+GGG + tig0026160:38790-38809 Msa1427100:intron 25.0%
!!! TTTTTTCTTTTACTTGGTGA+AGG - tig0026160:38832-38851 None:intergenic 25.0%
!!! GTTTCTAATTTTGACTTTGT+TGG + tig0026160:38891-38910 Msa1427100:intron 25.0%
!!! GGTTTTATTTTTTGAACTCA+AGG + tig0026160:38912-38931 Msa1427100:intron 25.0%
!!! TTTATTTTTTGAACTCAAGG+TGG + tig0026160:38915-38934 Msa1427100:intron 25.0%
!!! ATTTTTAGTAACAATGTTGG+AGG - tig0026160:39518-39537 None:intergenic 25.0%
!!! CACATTTTTAGTAACAATGT+TGG - tig0026160:39521-39540 None:intergenic 25.0%
! AAGCATTTGAATTATGAACA+AGG + tig0026160:39555-39574 Msa1427100:intron 25.0%
! AGCATTTGAATTATGAACAA+GGG + tig0026160:39556-39575 Msa1427100:intron 25.0%
!!! TGATTGGTTATGCTTTTATA+AGG + tig0026160:39688-39707 Msa1427100:intron 25.0%
!!! ACAAAATTCCACTTTTTCTA+AGG + tig0026160:39734-39753 Msa1427100:intron 25.0%
! ACGAAAATAATGATTCCAAA+TGG + tig0026160:40103-40122 Msa1427100:CDS 25.0%
! AAAATCATTGTGAATTCTGA+TGG + tig0026160:40168-40187 Msa1427100:CDS 25.0%
!!! TTTTTCAAATCCATCATCAT+TGG + tig0026160:40284-40303 Msa1427100:CDS 25.0%
! TTTGTCATAACATAGTAACA+AGG - tig0026160:40529-40548 None:intergenic 25.0%
! GACAAAAAAAAAGGGTAAAA+AGG + tig0026160:40543-40562 Msa1427100:three_prime_UTR 25.0%
!!! ATTTTTTAAAAAGGGTTGGT+TGG + tig0026160:40604-40623 Msa1427100:three_prime_UTR 25.0%
!!! TTTTTTAAAAAGGGTTGGTT+GGG + tig0026160:40605-40624 Msa1427100:three_prime_UTR 25.0%
! AGGATTAATGAATGAATGTT+AGG + tig0026160:40636-40655 Msa1427100:three_prime_UTR 25.0%
! GTTACATAAAGAAAACAAAG+TGG - tig0026160:40672-40691 None:intergenic 25.0%
!! TGTTTTCTTTATGTAACACA+AGG + tig0026160:40675-40694 Msa1427100:three_prime_UTR 25.0%
!! GTTTTCTTTATGTAACACAA+GGG + tig0026160:40676-40695 Msa1427100:three_prime_UTR 25.0%
! ATGAATGAGAATATGCATAT+TGG + tig0026160:40743-40762 Msa1427100:three_prime_UTR 25.0%
GAAGAGAAATAGATTCTGTT+AGG + tig0026160:37187-37206 Msa1427100:five_prime_UTR 30.0%
ACAAACTCAACAATCTGTTA+TGG + tig0026160:37449-37468 Msa1427100:CDS 30.0%
! TGAAAGTTGTTACCATTTTC+AGG - tig0026160:37479-37498 None:intergenic 30.0%
ATAGGCATAACAGTCAAATT+AGG - tig0026160:37506-37525 None:intergenic 30.0%
TCTTTAGATTCTACCTCTAA+TGG - tig0026160:38026-38045 None:intergenic 30.0%
! AGGAAATCTATTGACACTTT+TGG + tig0026160:38359-38378 Msa1427100:CDS 30.0%
GCTTTCTTAAGTTATCATGT+TGG + tig0026160:38532-38551 Msa1427100:intron 30.0%
!! ATCATGTTGGTGATTATCTT+TGG + tig0026160:38545-38564 Msa1427100:intron 30.0%
GTAGATATGAAGCACATTTA+TGG + tig0026160:38603-38622 Msa1427100:CDS 30.0%
!!! GTGTTATTTGTTCATGCTTT+TGG + tig0026160:38744-38763 Msa1427100:intron 30.0%
CATGCATGTTACAGTATATT+TGG + tig0026160:38789-38808 Msa1427100:intron 30.0%
AGAAACAGTAATAGCAATGA+TGG - tig0026160:38877-38896 None:intergenic 30.0%
AAGCTAACAAGAAAATGCTA+AGG - tig0026160:39039-39058 None:intergenic 30.0%
AGGAAATGAAGAATATGACA+AGG + tig0026160:39143-39162 Msa1427100:CDS 30.0%
AATATGTTGCACATTTGAGA+AGG + tig0026160:39170-39189 Msa1427100:CDS 30.0%
TATTATTGCAGAAAAAGCAG+TGG + tig0026160:39277-39296 Msa1427100:intron 30.0%
ATTTGAATTTGTCACACTGT+TGG + tig0026160:39369-39388 Msa1427100:intron 30.0%
TACTGAATGTTCCAAGATAA+AGG - tig0026160:39487-39506 None:intergenic 30.0%
ATAACCAATCACTTGTGTTT+GGG - tig0026160:39679-39698 None:intergenic 30.0%
!!! ACTTTTTCTAAGGCTGTAAA+GGG + tig0026160:39744-39763 Msa1427100:intron 30.0%
AAAGGGAATCTCTAATAGAA+TGG + tig0026160:39761-39780 Msa1427100:intron 30.0%
ATCTCTAATAGAATGGTGTA+AGG + tig0026160:39768-39787 Msa1427100:intron 30.0%
ATATGATGTTGAAAGGATCA+TGG + tig0026160:39942-39961 Msa1427100:CDS 30.0%
TAATGATTCCAAATGGAAGA+TGG + tig0026160:40110-40129 Msa1427100:CDS 30.0%
CAACTGAATTAATACCAACA+AGG - tig0026160:40233-40252 None:intergenic 30.0%
CTTGTTGGTATTAATTCAGT+TGG + tig0026160:40231-40250 Msa1427100:CDS 30.0%
TGGTATTAATTCAGTTGGTT+TGG + tig0026160:40236-40255 Msa1427100:CDS 30.0%
TTGGATGATTCTACCAAAAT+AGG + tig0026160:40255-40274 Msa1427100:CDS 30.0%
TGGATGATTCTACCAAAATA+GGG + tig0026160:40256-40275 Msa1427100:CDS 30.0%
! TGAAAAATGTGTCCCTATTT+TGG - tig0026160:40271-40290 None:intergenic 30.0%
!! GCAATTTTTGATTCCATTGA+AGG - tig0026160:40378-40397 None:intergenic 30.0%
! TGAATGAATGTTAGGACTTT+TGG + tig0026160:40644-40663 Msa1427100:three_prime_UTR 30.0%
! GAATGAATGTTAGGACTTTT+GGG + tig0026160:40645-40664 Msa1427100:three_prime_UTR 30.0%
! AATGAATGTTAGGACTTTTG+GGG + tig0026160:40646-40665 Msa1427100:three_prime_UTR 30.0%
AGAGTTACTACTGTCATTCA+TGG - tig0026160:37273-37292 None:intergenic 35.0%
AGGAGAAGAAGTAGAAATGT+GGG - tig0026160:37342-37361 None:intergenic 35.0%
! TGGTAATGATGATGATGATG+AGG - tig0026160:37362-37381 None:intergenic 35.0%
! CTGAAGAAGTTTGAGTTTGT+TGG - tig0026160:37382-37401 None:intergenic 35.0%
GGTGAAAAATAGAAAGAAGC+AGG - tig0026160:37419-37438 None:intergenic 35.0%
TGTTATGGTGTTCCTGAAAA+TGG + tig0026160:37464-37483 Msa1427100:CDS 35.0%
AGATGGATCACTTTGTATCA+TGG + tig0026160:37532-37551 Msa1427100:CDS 35.0%
! AGCACACAACACATTTTGAA+GGG - tig0026160:37625-37644 None:intergenic 35.0%
! AAGCACACAACACATTTTGA+AGG - tig0026160:37626-37645 None:intergenic 35.0%
! TCTAGTTTAGGAGATGAAGA+TGG - tig0026160:37780-37799 None:intergenic 35.0%
ATCTTCATCTCCTAAACTAG+AGG + tig0026160:37779-37798 Msa1427100:CDS 35.0%
! CCAAGAAAATCCTCTAGTTT+AGG - tig0026160:37792-37811 None:intergenic 35.0%
! CCTAAACTAGAGGATTTTCT+TGG + tig0026160:37789-37808 Msa1427100:CDS 35.0%
! AAACTAGAGGATTTTCTTGG+TGG + tig0026160:37792-37811 Msa1427100:CDS 35.0%
ACAATGGGTAGTGATGAATA+TGG + tig0026160:37819-37838 Msa1427100:CDS 35.0%
! AGATTCTGAAAGAAGGTCAA+GGG - tig0026160:37940-37959 None:intergenic 35.0%
! AAGATTCTGAAAGAAGGTCA+AGG - tig0026160:37941-37960 None:intergenic 35.0%
! GTCTGAAAGATTCTGAAAGA+AGG - tig0026160:37947-37966 None:intergenic 35.0%
! TAACCAAGTGCTGCATAATA+TGG - tig0026160:37984-38003 None:intergenic 35.0%
!!! ATACCAGTTTTGAGGCATTT+GGG - tig0026160:38078-38097 None:intergenic 35.0%
!!! AATACCAGTTTTGAGGCATT+TGG - tig0026160:38079-38098 None:intergenic 35.0%
ACTATGGAGGCTAAAAAAAG+AGG + tig0026160:38302-38321 Msa1427100:CDS 35.0%
TATTCTCCATTAGGCATAGA+TGG + tig0026160:38576-38595 Msa1427100:intron 35.0%
AAGGAAGGACAAACAAGAAA+AGG + tig0026160:38641-38660 Msa1427100:CDS 35.0%
CAAACAAGAAAAGGAAGACA+AGG + tig0026160:38650-38669 Msa1427100:CDS 35.0%
AACTCAAGGTGGTTATGATA+TGG + tig0026160:38926-38945 Msa1427100:intron 35.0%
ATCAAGCAGCTCTTAAGTAT+TGG + tig0026160:38973-38992 Msa1427100:CDS 35.0%
TCAAGCAGCTCTTAAGTATT+GGG + tig0026160:38974-38993 Msa1427100:CDS 35.0%
TTCAACACACATAAACTTCC+CGG + tig0026160:39001-39020 Msa1427100:CDS 35.0%
AAACTACCATACTCAACTTG+AGG + tig0026160:39123-39142 Msa1427100:CDS 35.0%
!! TCATTTCCTCAAGTTGAGTA+TGG - tig0026160:39132-39151 None:intergenic 35.0%
AATGAAGAATATGACAAGGC+AGG + tig0026160:39147-39166 Msa1427100:CDS 35.0%
! AAAAAGCAGTGGATTCTCAA+GGG + tig0026160:39288-39307 Msa1427100:CDS 35.0%
! TTTTCCCAAACACAAGTGAT+TGG + tig0026160:39672-39691 Msa1427100:intron 35.0%
CATAACCAATCACTTGTGTT+TGG - tig0026160:39680-39699 None:intergenic 35.0%
CTTTACAGCCTTAGAAAAAG+TGG - tig0026160:39745-39764 None:intergenic 35.0%
!!! CACTTTTTCTAAGGCTGTAA+AGG + tig0026160:39743-39762 Msa1427100:intron 35.0%
! TGAAATTTTAGGCACTCAAG+AGG + tig0026160:39843-39862 Msa1427100:intron 35.0%
TGCAAGGAGAATCAAAGATA+AGG + tig0026160:39993-40012 Msa1427100:CDS 35.0%
ATGTCTTTACAAGACCTTGT+TGG + tig0026160:40216-40235 Msa1427100:CDS 35.0%
AAACTTGTCACCAATGATGA+TGG - tig0026160:40297-40316 None:intergenic 35.0%
TTTGATTCCATTGAAGGCTT+TGG - tig0026160:40372-40391 None:intergenic 35.0%
AGAACTAACAGCAACATTAG+TGG - tig0026160:40400-40419 None:intergenic 35.0%
! CTAATGTTGCTGTTAGTTCT+TGG + tig0026160:40400-40419 Msa1427100:CDS 35.0%
ATTTGTGTAGCTACTTGTGA+AGG - tig0026160:40429-40448 None:intergenic 35.0%
GACAAATTGATTGAAGATGC+TGG - tig0026160:40456-40475 None:intergenic 35.0%
CAAGGAGATATAATCTCTCT+AGG - tig0026160:40511-40530 None:intergenic 35.0%
ATGTAACACAAGGGTAACAT+TGG + tig0026160:40685-40704 Msa1427100:three_prime_UTR 35.0%
!! CCAAAAGGCTAAGTTTGTTA+AGG - tig0026160:40711-40730 None:intergenic 35.0%
!! CCTTAACAAACTTAGCCTTT+TGG + tig0026160:40708-40727 Msa1427100:three_prime_UTR 35.0%
!! TTAGCCTTTTGGCTATATGT+TGG + tig0026160:40719-40738 Msa1427100:three_prime_UTR 35.0%
CATTCCAACATATAGCCAAA+AGG - tig0026160:40726-40745 None:intergenic 35.0%
AAGCTGAAGCAGAAGAATAG+AGG + tig0026160:37084-37103 Msa1427100:five_prime_UTR 40.0%
GGAGAAGAAGTAGAAATGTG+GGG - tig0026160:37341-37360 None:intergenic 40.0%
GAGGAGAAGAAGTAGAAATG+TGG - tig0026160:37343-37362 None:intergenic 40.0%
GTTGAGTTTGTGAAGTGAGA+AGG - tig0026160:37440-37459 None:intergenic 40.0%
GTTATGCCTATCAAGTCAGA+TGG + tig0026160:37515-37534 Msa1427100:CDS 40.0%
! AGTGATCCATCTGACTTGAT+AGG - tig0026160:37524-37543 None:intergenic 40.0%
CTTTGTATCATGGAAGCTCT+TGG + tig0026160:37542-37561 Msa1427100:CDS 40.0%
CTTGGTAGATCACAATCACA+AGG + tig0026160:37560-37579 Msa1427100:CDS 40.0%
GTAGATCACAATCACAAGGT+TGG + tig0026160:37564-37583 Msa1427100:intron 40.0%
!!! TTTTCTTGGTGGTGCAACAA+TGG + tig0026160:37803-37822 Msa1427100:CDS 40.0%
!! TTTCTTGGTGGTGCAACAAT+GGG + tig0026160:37804-37823 Msa1427100:CDS 40.0%
ATATGGTAGCCATGAAAGTG+AGG + tig0026160:37836-37855 Msa1427100:CDS 40.0%
! GATGAGGATCTGCATTTTGT+TGG - tig0026160:37899-37918 None:intergenic 40.0%
! TGGTCTGTATTAGCTTGATG+AGG - tig0026160:37915-37934 None:intergenic 40.0%
CATCCATATTATGCAGCACT+TGG + tig0026160:37978-37997 Msa1427100:CDS 40.0%
TATGCAGCACTTGGTTATCA+TGG + tig0026160:37987-38006 Msa1427100:CDS 40.0%
CCTCTAATGGTGCTTGAAAT+AGG - tig0026160:38013-38032 None:intergenic 40.0%
CCTATTTCAAGCACCATTAG+AGG + tig0026160:38010-38029 Msa1427100:CDS 40.0%
GGGAACTAGAAACATCAACA+TGG - tig0026160:38058-38077 None:intergenic 40.0%
! GAAGCAGAATACCAGTTTTG+AGG - tig0026160:38086-38105 None:intergenic 40.0%
CAGATGAACACTACTAGCAT+GGG + tig0026160:38122-38141 Msa1427100:CDS 40.0%
AGATGAACACTACTAGCATG+GGG + tig0026160:38123-38142 Msa1427100:CDS 40.0%
TCTTCTGTTGTTCGTACTGT+AGG + tig0026160:38176-38195 Msa1427100:CDS 40.0%
TGGAACAGAATCAGTGACTA+TGG + tig0026160:38286-38305 Msa1427100:CDS 40.0%
AACAGAATCAGTGACTATGG+AGG + tig0026160:38289-38308 Msa1427100:CDS 40.0%
GTCAAAAGCAGCCTATTCAT+AGG + tig0026160:38339-38358 Msa1427100:CDS 40.0%
CAGAGAACATCTCAGTATAG+AGG + tig0026160:38383-38402 Msa1427100:CDS 40.0%
ATGGGATAACAGTTGCAAGA+AGG + tig0026160:38622-38641 Msa1427100:CDS 40.0%
GATAACAGTTGCAAGAAGGA+AGG + tig0026160:38626-38645 Msa1427100:CDS 40.0%
! CAAGCAGCTCTTAAGTATTG+GGG + tig0026160:38975-38994 Msa1427100:CDS 40.0%
GGGAAGTTTATGTGTGTTGA+AGG - tig0026160:39002-39021 None:intergenic 40.0%
CTAAGGAGATCAAATGTACC+GGG - tig0026160:39022-39041 None:intergenic 40.0%
GCTAAGGAGATCAAATGTAC+CGG - tig0026160:39023-39042 None:intergenic 40.0%
! GAAAAAGCAGTGGATTCTCA+AGG + tig0026160:39287-39306 Msa1427100:intron 40.0%
! AAAAGCAGTGGATTCTCAAG+GGG + tig0026160:39289-39308 Msa1427100:CDS 40.0%
! TTTTGCAGACATCACCAACA+TGG + tig0026160:39419-39438 Msa1427100:intron 40.0%
GGTAACAAGGACCTTTATCT+TGG + tig0026160:39473-39492 Msa1427100:CDS 40.0%
GTAGCAGCAATAAAATTCCG+TGG + tig0026160:39886-39905 Msa1427100:CDS 40.0%
TTGTAACAGCATTTGCTCCA+CGG - tig0026160:39906-39925 None:intergenic 40.0%
GCTAGCAATACTCTTCTTTC+TGG + tig0026160:39964-39983 Msa1427100:CDS 40.0%
TATTCATGAACTTCGGTCTG+TGG - tig0026160:40021-40040 None:intergenic 40.0%
CTGCAAAACCATCTTCCATT+TGG - tig0026160:40121-40140 None:intergenic 40.0%
ACTCTTTCCAAAGCCTTCAA+TGG + tig0026160:40362-40381 Msa1427100:CDS 40.0%
CACAAGTAGCTACACAAATG+AGG + tig0026160:40430-40449 Msa1427100:CDS 40.0%
CATTCCATGCAGCAAAAACT+GGG - tig0026160:40485-40504 None:intergenic 40.0%
TCATTCCATGCAGCAAAAAC+TGG - tig0026160:40486-40505 None:intergenic 40.0%
!! AAAGGGTTGGTTGGGAAATT+TGG + tig0026160:40613-40632 Msa1427100:three_prime_UTR 40.0%
GAAAGAAGCAGGAACAGTGT+TGG - tig0026160:37408-37427 None:intergenic 45.0%
TAGCCATGAAAGTGAGGCAA+TGG + tig0026160:37842-37861 Msa1427100:CDS 45.0%
AAGCCATTGCCTCACTTTCA+TGG - tig0026160:37848-37867 None:intergenic 45.0%
CTGAAAGAAGGTCAAGGGAA+TGG - tig0026160:37935-37954 None:intergenic 45.0%
GTTCCCAAATGCCTCAAAAC+TGG + tig0026160:38072-38091 Msa1427100:CDS 45.0%
GCAGATGAACACTACTAGCA+TGG + tig0026160:38121-38140 Msa1427100:CDS 45.0%
ACTAGCATGGGGAGTCATAA+TGG + tig0026160:38134-38153 Msa1427100:CDS 45.0%
GTTGTTCGTACTGTAGGAAG+TGG + tig0026160:38182-38201 Msa1427100:CDS 45.0%
TTAAGCCTTTCAATGAGCCC+TGG + tig0026160:38218-38237 Msa1427100:CDS 45.0%
CACAGCTAGATTGAGAACCA+GGG - tig0026160:38238-38257 None:intergenic 45.0%
ACACAGCTAGATTGAGAACC+AGG - tig0026160:38239-38258 None:intergenic 45.0%
!! GTCACTGATTCTGTTCCAGA+AGG - tig0026160:38284-38303 None:intergenic 45.0%
AAAAGAGGAGCTGCTAAGCT+TGG + tig0026160:38317-38336 Msa1427100:CDS 45.0%
!! CTCAGTATAGAGGTGTCACA+AGG + tig0026160:38393-38412 Msa1427100:CDS 45.0%
TCCATTAGGCATAGATGGAC+TGG + tig0026160:38581-38600 Msa1427100:intron 45.0%
ACCAGTCCATCTATGCCTAA+TGG - tig0026160:38585-38604 None:intergenic 45.0%
TGGAAGAGTTGCTGGTAACA+AGG + tig0026160:39460-39479 Msa1427100:CDS 45.0%
! TTCTTTCTGGTCAGCATGCA+AGG + tig0026160:39977-39996 Msa1427100:CDS 45.0%
AATGTATCGAGCCAGACCAA+TGG + tig0026160:40054-40073 Msa1427100:CDS 45.0%
!! AGTGAGGGAGCAGTTTTATC+AGG - tig0026160:40342-40361 None:intergenic 45.0%
!! GGCTTTGGAAAGAGTAGTGA+GGG - tig0026160:40357-40376 None:intergenic 45.0%
!! AGGCTTTGGAAAGAGTAGTG+AGG - tig0026160:40358-40377 None:intergenic 45.0%
! ACTTCCCAGTTTTTGCTGCA+TGG + tig0026160:40478-40497 Msa1427100:CDS 45.0%
AGCATGGGGAGTCATAATGG+TGG + tig0026160:38137-38156 Msa1427100:CDS 50.0%
! AGTCATAATGGTGGAGCTGG+TGG + tig0026160:38146-38165 Msa1427100:CDS 50.0%
GAGAACCAGGGCTCATTGAA+AGG - tig0026160:38226-38245 None:intergenic 50.0%
CCAGAAGGTGAGATCTGTCT+AGG - tig0026160:38269-38288 None:intergenic 50.0%
CCTAGACAGATCTCACCTTC+TGG + tig0026160:38266-38285 Msa1427100:CDS 50.0%
! AGGGGTGCATCAATGTACAG+AGG + tig0026160:39307-39326 Msa1427100:CDS 50.0%
! CAATGTACAGAGGCGTGACA+AGG + tig0026160:39317-39336 Msa1427100:CDS 50.0%
GCAGACATCACCAACATGGT+AGG + tig0026160:39423-39442 Msa1427100:intron 50.0%
GACATCACCAACATGGTAGG+TGG + tig0026160:39426-39445 Msa1427100:intron 50.0%
TTGCTTGCCACCTACCATGT+TGG - tig0026160:39436-39455 None:intergenic 50.0%
AACATGGTAGGTGGCAAGCA+AGG + tig0026160:39435-39454 Msa1427100:CDS 50.0%
GCAAGGATTGGAAGAGTTGC+TGG + tig0026160:39452-39471 Msa1427100:CDS 50.0%
!! GGGTTGGTTGGGAAATTTGG+AGG + tig0026160:40616-40635 Msa1427100:three_prime_UTR 50.0%
! GGGAGTCATAATGGTGGAGC+TGG + tig0026160:38143-38162 Msa1427100:CDS 55.0%
! CATAATGGTGGAGCTGGTGG+TGG + tig0026160:38149-38168 Msa1427100:CDS 55.0%
GGTAGGTGGCAAGCAAGGAT+TGG + tig0026160:39440-39459 Msa1427100:CDS 55.0%
CAGCAGCTTCTCCATTGGTC+TGG - tig0026160:40068-40087 None:intergenic 55.0%
CGCTTCAGCAGCTTCTCCAT+TGG - tig0026160:40073-40092 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0026160 gene 36964 40767 36964 ID=Msa1427100;Name=Msa1427100
tig0026160 mRNA 36964 40767 36964 ID=Msa1427100-mRNA-1;Parent=Msa1427100;Name=Msa1427100-mRNA-1;_AED=0.04;_eAED=0.04;_QI=299|0.85|0.75|1|1|1|8|255|665
tig0026160 exon 36964 37581 36964 ID=Msa1427100-mRNA-1:exon:5268;Parent=Msa1427100-mRNA-1
tig0026160 exon 37769 38414 37769 ID=Msa1427100-mRNA-1:exon:5269;Parent=Msa1427100-mRNA-1
tig0026160 exon 38589 38671 38589 ID=Msa1427100-mRNA-1:exon:5270;Parent=Msa1427100-mRNA-1
tig0026160 exon 38934 39022 38934 ID=Msa1427100-mRNA-1:exon:5271;Parent=Msa1427100-mRNA-1
tig0026160 exon 39118 39191 39118 ID=Msa1427100-mRNA-1:exon:5272;Parent=Msa1427100-mRNA-1
tig0026160 exon 39288 39338 39288 ID=Msa1427100-mRNA-1:exon:5273;Parent=Msa1427100-mRNA-1
tig0026160 exon 39427 39503 39427 ID=Msa1427100-mRNA-1:exon:5274;Parent=Msa1427100-mRNA-1
tig0026160 exon 39854 40767 39854 ID=Msa1427100-mRNA-1:exon:5275;Parent=Msa1427100-mRNA-1
tig0026160 five_prime_UTR 36964 37262 36964 ID=Msa1427100-mRNA-1:five_prime_utr;Parent=Msa1427100-mRNA-1
tig0026160 CDS 37263 37581 37263 ID=Msa1427100-mRNA-1:cds;Parent=Msa1427100-mRNA-1
tig0026160 CDS 37769 38414 37769 ID=Msa1427100-mRNA-1:cds;Parent=Msa1427100-mRNA-1
tig0026160 CDS 38589 38671 38589 ID=Msa1427100-mRNA-1:cds;Parent=Msa1427100-mRNA-1
tig0026160 CDS 38934 39022 38934 ID=Msa1427100-mRNA-1:cds;Parent=Msa1427100-mRNA-1
tig0026160 CDS 39118 39191 39118 ID=Msa1427100-mRNA-1:cds;Parent=Msa1427100-mRNA-1
tig0026160 CDS 39288 39338 39288 ID=Msa1427100-mRNA-1:cds;Parent=Msa1427100-mRNA-1
tig0026160 CDS 39427 39503 39427 ID=Msa1427100-mRNA-1:cds;Parent=Msa1427100-mRNA-1
tig0026160 CDS 39854 40512 39854 ID=Msa1427100-mRNA-1:cds;Parent=Msa1427100-mRNA-1
tig0026160 three_prime_UTR 40513 40767 40513 ID=Msa1427100-mRNA-1:three_prime_utr;Parent=Msa1427100-mRNA-1
Gene Sequence

>Msa1427100

ATGAAGTCCATGAATGACAGTAGTAACTCTGATGACAACAATCATAACAACAATTGGTTAGGTTTCTCTCTCTCACCCCACATTTCTACTTCTTCTCCTCATCATCATCATCATTACCAACAAACTCAAACTTCTTCAGTTTCCAACACTGTTCCTGCTTCTTTCTATTTTTCACCTTCTCACTTCACAAACTCAACAATCTGTTATGGTGTTCCTGAAAATGGTAACAACTTTCATTCACCTAATTTGACTGTTATGCCTATCAAGTCAGATGGATCACTTTGTATCATGGAAGCTCTTGGTAGATCACAATCACAAGTGATGGTGCCATCTTCATCTCCTAAACTAGAGGATTTTCTTGGTGGTGCAACAATGGGTAGTGATGAATATGGTAGCCATGAAAGTGAGGCAATGGCTTTAAGCTTAGACAGCATCTACTATAATAACCAACAAAATGCAGATCCTCATCAAGCTAATACAGACCATTCCCTTGACCTTCTTTCAGAATCTTTCAGACAACAAACATCACATCCATATTATGCAGCACTTGGTTATCATGGCCTATTTCAAGCACCATTAGAGGTAGAATCTAAAGAAAACATAAACCATGTTGATGTTTCTAGTTCCCAAATGCCTCAAAACTGGTATTCTGCTTCTCAAGCTTTAGAGCAGCAGATGAACACTACTAGCATGGGGAGTCATAATGGTGGAGCTGGTGGTGGTTCTTCTTCTGTTGTTCGTACTGTAGGAAGTGGCGAGTTACAATCTTTAAGCCTTTCAATGAGCCCTGGTTCTCAATCTAGCTGTGTTACTGTTCCTAGACAGATCTCACCTTCTGGAACAGAATCAGTGACTATGGAGGCTAAAAAAAGAGGAGCTGCTAAGCTTGGTCAAAAGCAGCCTATTCATAGGAAATCTATTGACACTTTTGGACAGAGAACATCTCAGTATAGAGGTGTCACAAGGCATAGATGGACTGGTAGATATGAAGCACATTTATGGGATAACAGTTGCAAGAAGGAAGGACAAACAAGAAAAGGAAGACAAGGTGGTTATGATATGGAAGAGAAAGCTGCAAGAGCTTATGATCAAGCAGCTCTTAAGTATTGGGGACCTTCAACACACATAAACTTCCCGCTAGAAAACTACCATACTCAACTTGAGGAAATGAAGAATATGACAAGGCAGGAATATGTTGCACATTTGAGAAGAAAAAGCAGTGGATTCTCAAGGGGTGCATCAATGTACAGAGGCGTGACAAGACATCACCAACATGGTAGGTGGCAAGCAAGGATTGGAAGAGTTGCTGGTAACAAGGACCTTTATCTTGGAACATTCAGCACTCAAGAGGAAGCAGCTGAAGCATATGATGTAGCAGCAATAAAATTCCGTGGAGCAAATGCTGTTACAAATTTTGACATATCTAAATATGATGTTGAAAGGATCATGGCTAGCAATACTCTTCTTTCTGGTCAGCATGCAAGGAGAATCAAAGATAAGGATCCACAGACCGAAGTTCATGAATATAATCATAGTACTAATGTATCGAGCCAGACCAATGGAGAAGCTGCTGAAGCGCAAAACGTTAACGAAAATAATGATTCCAAATGGAAGATGGTTTTGCAGCAACAGCAATCAAATAGTTGTGATAAAAAAATCATTGTGAATTCTGATGGAAGTTACAAGAATTCTGATTTCTCTATGTCTTTACAAGACCTTGTTGGTATTAATTCAGTTGGTTTGGATGATTCTACCAAAATAGGGACACATTTTTCAAATCCATCATCATTGGTGACAAGTTTAAGCAGTTCAAGAGAAGCTAGTCCTGATAAAACTGCTCCCTCACTACTCTTTCCAAAGCCTTCAATGGAATCAAAAATTGCCACTAATGTTGCTGTTAGTTCTTGGTTTCCTTCACAAGTAGCTACACAAATGAGGCCAGCATCTTCAATCAATTTGTCTCACTTCCCAGTTTTTGCTGCATGGAATGAGACCTAG
Protein sequence

>Msa1427100

MKSMNDSSNSDDNNHNNNWLGFSLSPHISTSSPHHHHHYQQTQTSSVSNTVPASFYFSPSHFTNSTICYGVPENGNNFHSPNLTVMPIKSDGSLCIMEALGRSQSQVMVPSSSPKLEDFLGGATMGSDEYGSHESEAMALSLDSIYYNNQQNADPHQANTDHSLDLLSESFRQQTSHPYYAALGYHGLFQAPLEVESKENINHVDVSSSQMPQNWYSASQALEQQMNTTSMGSHNGGAGGGSSSVVRTVGSGELQSLSLSMSPGSQSSCVTVPRQISPSGTESVTMEAKKRGAAKLGQKQPIHRKSIDTFGQRTSQYRGVTRHRWTGRYEAHLWDNSCKKEGQTRKGRQGGYDMEEKAARAYDQAALKYWGPSTHINFPLENYHTQLEEMKNMTRQEYVAHLRRKSSGFSRGASMYRGVTRHHQHGRWQARIGRVAGNKDLYLGTFSTQEEAAEAYDVAAIKFRGANAVTNFDISKYDVERIMASNTLLSGQHARRIKDKDPQTEVHEYNHSTNVSSQTNGEAAEAQNVNENNDSKWKMVLQQQQSNSCDKKIIVNSDGSYKNSDFSMSLQDLVGINSVGLDDSTKIGTHFSNPSSLVTSLSSSREASPDKTAPSLLFPKPSMESKIATNVAVSSWFPSQVATQMRPASSINLSHFPVFAAWNET*