AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1226840


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1226840 MtrunA17_Chr8g0335231 88.690 168 9 1 1 158 1 168 1.83e-102 291
Msa1226840 MtrunA17_Chr4g0052031 50.400 125 52 4 34 150 86 208 8.21e-32 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1226840 AT4G37445.1 49.686 159 69 2 6 153 3 161 2.43e-46 149
Msa1226840 AT4G37445.2 50.694 144 61 1 20 153 2 145 1.61e-43 141

Find 30 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAAAAGTGAAAATTAAAT+AGG 0.234371 8_2:-1860142 None:intergenic
TATATACACAAATATATATT+TGG 0.268577 8_2:+1858998 Msa1226840:five_prime_UTR
CATTGATTTCATGATCAATA+AGG 0.282087 8_2:+1859913 Msa1226840:CDS
TACCATCACACAAGAAATTA+AGG 0.290774 8_2:+1859835 Msa1226840:intron
TTTCTTCAATGACATACTTT+TGG 0.319686 8_2:-1860113 None:intergenic
GATGATTCTATGATGATAAC+TGG 0.356747 8_2:+1859953 Msa1226840:CDS
CAGACATTAGATCTTGTATC+AGG 0.382635 8_2:+1859251 Msa1226840:CDS
CTGATACAAGATCTAATGTC+TGG 0.398337 8_2:-1859250 None:intergenic
ATGAGTTACATGGGAGGATC+AGG 0.435033 8_2:+1859073 Msa1226840:CDS
TATGATGATAACTGGAATAG+TGG 0.452300 8_2:+1859961 Msa1226840:CDS
GCTATGGTAGCTAAGAGAAC+AGG 0.456395 8_2:+1859995 Msa1226840:CDS
CTCTTAGCTACCATAGCTGC+TGG 0.471596 8_2:-1859989 None:intergenic
ATGTTTCTTCTTATCTTCCT+CGG 0.474190 8_2:-1859885 None:intergenic
ACTATTAACATGGCCTTGCC+TGG 0.488789 8_2:+1859798 Msa1226840:CDS
ATCATAATGCTTACCAGGCA+AGG 0.514482 8_2:-1859811 None:intergenic
GAGCTAAAATAGTAGCTGTT+GGG 0.514762 8_2:-1860072 None:intergenic
GTTGGGACAAAGATAACATC+AGG 0.516476 8_2:-1860055 None:intergenic
ATCAAGAATGGCAGTGTGGA+AGG 0.518421 8_2:-1859321 None:intergenic
AGAGCTAAAATAGTAGCTGT+TGG 0.518631 8_2:-1860073 None:intergenic
AGTGGCACCTCCAGCAGCTA+TGG 0.520301 8_2:+1859979 Msa1226840:CDS
TTACTTGAAGATATCAAGAA+TGG 0.537162 8_2:-1859333 None:intergenic
GGGGATGATCATGAGTTACA+TGG 0.552167 8_2:+1859063 Msa1226840:CDS
GACAAAGATAACATCAGGAA+TGG 0.591291 8_2:-1860050 None:intergenic
TGATACAAGATCTAATGTCT+GGG 0.602501 8_2:-1859249 None:intergenic
TTAGCTACCATAGCTGCTGG+AGG 0.629958 8_2:-1859986 None:intergenic
GCTTTCATAACACTTAGTTG+AGG 0.635041 8_2:-1860028 None:intergenic
GGGATGATCATGAGTTACAT+GGG 0.647259 8_2:+1859064 Msa1226840:CDS
AAACTGGAATAAAACATCCG+AGG 0.684088 8_2:+1859868 Msa1226840:CDS
AGATATCAAGAATGGCAGTG+TGG 0.711163 8_2:-1859325 None:intergenic
ATGATCATGAGTTACATGGG+AGG 0.727517 8_2:+1859067 Msa1226840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATATTTTAAGAAAAAATG+TGG - chr8_2:1858983-1859002 None:intergenic 10.0%
!! TATATACACAAATATATATT+TGG + chr8_2:1858998-1859017 Msa1226840:five_prime_UTR 10.0%
!! AATAAAAAAAAAAAAACTAG+TGG - chr8_2:1859130-1859149 None:intergenic 10.0%
!! ACTTGTAAAAAAAAAAAAAA+AGG + chr8_2:1859389-1859408 Msa1226840:intron 10.0%
!!! ATATATTTTAAGAAAAAATG+TGG - chr8_2:1858983-1859002 None:intergenic 10.0%
!! TATATACACAAATATATATT+TGG + chr8_2:1858998-1859017 Msa1226840:five_prime_UTR 10.0%
!! AATAAAAAAAAAAAAACTAG+TGG - chr8_2:1859130-1859149 None:intergenic 10.0%
!! ACTTGTAAAAAAAAAAAAAA+AGG + chr8_2:1859389-1859408 Msa1226840:intron 10.0%
!! AAAAAATACAAAAACATCAT+GGG + chr8_2:1859043-1859062 Msa1226840:exon 15.0%
!!! GTTTTTTTTTTTTTATTGCT+AGG + chr8_2:1859133-1859152 Msa1226840:intron 15.0%
!! AAAATTTAACATATTTCATC+AGG + chr8_2:1859212-1859231 Msa1226840:intron 15.0%
!! AAAAAAAAAAAAAAGGAAAG+TGG + chr8_2:1859396-1859415 Msa1226840:intron 15.0%
!! AAAAAAAAAAAAAGGAAAGT+GGG + chr8_2:1859397-1859416 Msa1226840:intron 15.0%
!!! TCAAATAATCATCTAAAATA+TGG + chr8_2:1859512-1859531 Msa1226840:intron 15.0%
!! AAAAAATACAAAAACATCAT+GGG + chr8_2:1859043-1859062 Msa1226840:exon 15.0%
!!! GTTTTTTTTTTTTTATTGCT+AGG + chr8_2:1859133-1859152 Msa1226840:intron 15.0%
!! AAAATTTAACATATTTCATC+AGG + chr8_2:1859212-1859231 Msa1226840:intron 15.0%
!! AAAAAAAAAAAAAAGGAAAG+TGG + chr8_2:1859396-1859415 Msa1226840:intron 15.0%
!! AAAAAAAAAAAAAGGAAAGT+GGG + chr8_2:1859397-1859416 Msa1226840:intron 15.0%
!!! TCAAATAATCATCTAAAATA+TGG + chr8_2:1859512-1859531 Msa1226840:intron 15.0%
!! CAAAAAATACAAAAACATCA+TGG + chr8_2:1859042-1859061 Msa1226840:exon 20.0%
!! AAAAATACAAAAACATCATG+GGG + chr8_2:1859044-1859063 Msa1226840:exon 20.0%
!! AAAAAAACTAGTGGAATTAA+AGG - chr8_2:1859121-1859140 None:intergenic 20.0%
!! AAAAAAAAAAAAGGAAAGTG+GGG + chr8_2:1859398-1859417 Msa1226840:intron 20.0%
!! CAAACTATATACTAGTATAA+TGG + chr8_2:1859453-1859472 Msa1226840:intron 20.0%
!! TTGATATCAAATGTTGAATA+TGG - chr8_2:1859733-1859752 None:intergenic 20.0%
!! TTCAACATTTGATATCAATT+TGG + chr8_2:1859735-1859754 Msa1226840:intron 20.0%
!! CAAAAAATACAAAAACATCA+TGG + chr8_2:1859042-1859061 Msa1226840:exon 20.0%
!! AAAAATACAAAAACATCATG+GGG + chr8_2:1859044-1859063 Msa1226840:exon 20.0%
!! AAAAAAACTAGTGGAATTAA+AGG - chr8_2:1859121-1859140 None:intergenic 20.0%
!! AAAAAAAAAAAAGGAAAGTG+GGG + chr8_2:1859398-1859417 Msa1226840:intron 20.0%
!! CAAACTATATACTAGTATAA+TGG + chr8_2:1859453-1859472 Msa1226840:intron 20.0%
!! TTGATATCAAATGTTGAATA+TGG - chr8_2:1859733-1859752 None:intergenic 20.0%
!! TTCAACATTTGATATCAATT+TGG + chr8_2:1859735-1859754 Msa1226840:intron 20.0%
!!! TATATATTTGGTAGCTTTTG+TGG + chr8_2:1859010-1859029 Msa1226840:five_prime_UTR 25.0%
! TTACTTGAAGATATCAAGAA+TGG - chr8_2:1859336-1859355 None:intergenic 25.0%
!! AATCATCTAAAATATGGTCA+TGG + chr8_2:1859518-1859537 Msa1226840:intron 25.0%
! ATATGGTCATGGTAAAATTA+AGG + chr8_2:1859529-1859548 Msa1226840:intron 25.0%
!! GATTATCATTTTGTCAAAAG+TGG - chr8_2:1859621-1859640 None:intergenic 25.0%
! CCTTAGTTGCATAAATTTAA+GGG - chr8_2:1859672-1859691 None:intergenic 25.0%
! ACCTTAGTTGCATAAATTTA+AGG - chr8_2:1859673-1859692 None:intergenic 25.0%
!! TTTTACTAGCACTATTAACA+TGG + chr8_2:1859788-1859807 Msa1226840:intron 25.0%
!!! CATTGATTTCATGATCAATA+AGG + chr8_2:1859913-1859932 Msa1226840:CDS 25.0%
!! TTTCTTCAATGACATACTTT+TGG - chr8_2:1860116-1860135 None:intergenic 25.0%
!!! TATATATTTGGTAGCTTTTG+TGG + chr8_2:1859010-1859029 Msa1226840:five_prime_UTR 25.0%
! TTACTTGAAGATATCAAGAA+TGG - chr8_2:1859336-1859355 None:intergenic 25.0%
!! AATCATCTAAAATATGGTCA+TGG + chr8_2:1859518-1859537 Msa1226840:intron 25.0%
! ATATGGTCATGGTAAAATTA+AGG + chr8_2:1859529-1859548 Msa1226840:intron 25.0%
!! GATTATCATTTTGTCAAAAG+TGG - chr8_2:1859621-1859640 None:intergenic 25.0%
! CCTTAGTTGCATAAATTTAA+GGG - chr8_2:1859672-1859691 None:intergenic 25.0%
! ACCTTAGTTGCATAAATTTA+AGG - chr8_2:1859673-1859692 None:intergenic 25.0%
!! TTTTACTAGCACTATTAACA+TGG + chr8_2:1859788-1859807 Msa1226840:intron 25.0%
!!! CATTGATTTCATGATCAATA+AGG + chr8_2:1859913-1859932 Msa1226840:CDS 25.0%
!! TTTCTTCAATGACATACTTT+TGG - chr8_2:1860116-1860135 None:intergenic 25.0%
TTAAGAAAAAATGTGGACCT+AGG - chr8_2:1858976-1858995 None:intergenic 30.0%
TTAACATATTTCATCAGGTG+AGG + chr8_2:1859217-1859236 Msa1226840:intron 30.0%
TAACATATTTCATCAGGTGA+GGG + chr8_2:1859218-1859237 Msa1226840:intron 30.0%
TGATACAAGATCTAATGTCT+GGG - chr8_2:1859252-1859271 None:intergenic 30.0%
CCCTTAAATTTATGCAACTA+AGG + chr8_2:1859669-1859688 Msa1226840:intron 30.0%
TACCATCACACAAGAAATTA+AGG + chr8_2:1859835-1859854 Msa1226840:intron 30.0%
TTCCTTAATTTCTTGTGTGA+TGG - chr8_2:1859840-1859859 None:intergenic 30.0%
! TTAAGGAAGTTTTCGAAAAC+TGG + chr8_2:1859852-1859871 Msa1226840:CDS 30.0%
ATGTTTCTTCTTATCTTCCT+CGG - chr8_2:1859888-1859907 None:intergenic 30.0%
GATGATTCTATGATGATAAC+TGG + chr8_2:1859953-1859972 Msa1226840:CDS 30.0%
TATGATGATAACTGGAATAG+TGG + chr8_2:1859961-1859980 Msa1226840:CDS 30.0%
TTAAGAAAAAATGTGGACCT+AGG - chr8_2:1858976-1858995 None:intergenic 30.0%
TTAACATATTTCATCAGGTG+AGG + chr8_2:1859217-1859236 Msa1226840:intron 30.0%
TAACATATTTCATCAGGTGA+GGG + chr8_2:1859218-1859237 Msa1226840:intron 30.0%
TGATACAAGATCTAATGTCT+GGG - chr8_2:1859252-1859271 None:intergenic 30.0%
CCCTTAAATTTATGCAACTA+AGG + chr8_2:1859669-1859688 Msa1226840:intron 30.0%
TACCATCACACAAGAAATTA+AGG + chr8_2:1859835-1859854 Msa1226840:intron 30.0%
TTCCTTAATTTCTTGTGTGA+TGG - chr8_2:1859840-1859859 None:intergenic 30.0%
! TTAAGGAAGTTTTCGAAAAC+TGG + chr8_2:1859852-1859871 Msa1226840:CDS 30.0%
ATGTTTCTTCTTATCTTCCT+CGG - chr8_2:1859888-1859907 None:intergenic 30.0%
GATGATTCTATGATGATAAC+TGG + chr8_2:1859953-1859972 Msa1226840:CDS 30.0%
TATGATGATAACTGGAATAG+TGG + chr8_2:1859961-1859980 Msa1226840:CDS 30.0%
CTGATACAAGATCTAATGTC+TGG - chr8_2:1859253-1859272 None:intergenic 35.0%
CAGACATTAGATCTTGTATC+AGG + chr8_2:1859251-1859270 Msa1226840:CDS 35.0%
TACATACCATGCATGACAAA+AGG - chr8_2:1859594-1859613 None:intergenic 35.0%
AAACTGGAATAAAACATCCG+AGG + chr8_2:1859868-1859887 Msa1226840:CDS 35.0%
GCTTTCATAACACTTAGTTG+AGG - chr8_2:1860031-1860050 None:intergenic 35.0%
GACAAAGATAACATCAGGAA+TGG - chr8_2:1860053-1860072 None:intergenic 35.0%
GAGCTAAAATAGTAGCTGTT+GGG - chr8_2:1860075-1860094 None:intergenic 35.0%
AGAGCTAAAATAGTAGCTGT+TGG - chr8_2:1860076-1860095 None:intergenic 35.0%
CTGATACAAGATCTAATGTC+TGG - chr8_2:1859253-1859272 None:intergenic 35.0%
CAGACATTAGATCTTGTATC+AGG + chr8_2:1859251-1859270 Msa1226840:CDS 35.0%
TACATACCATGCATGACAAA+AGG - chr8_2:1859594-1859613 None:intergenic 35.0%
AAACTGGAATAAAACATCCG+AGG + chr8_2:1859868-1859887 Msa1226840:CDS 35.0%
GCTTTCATAACACTTAGTTG+AGG - chr8_2:1860031-1860050 None:intergenic 35.0%
GACAAAGATAACATCAGGAA+TGG - chr8_2:1860053-1860072 None:intergenic 35.0%
GAGCTAAAATAGTAGCTGTT+GGG - chr8_2:1860075-1860094 None:intergenic 35.0%
AGAGCTAAAATAGTAGCTGT+TGG - chr8_2:1860076-1860095 None:intergenic 35.0%
GGGATGATCATGAGTTACAT+GGG + chr8_2:1859064-1859083 Msa1226840:CDS 40.0%
ATGATCATGAGTTACATGGG+AGG + chr8_2:1859067-1859086 Msa1226840:CDS 40.0%
AGATATCAAGAATGGCAGTG+TGG - chr8_2:1859328-1859347 None:intergenic 40.0%
! ACCAAACCTTTTGTCATGCA+TGG + chr8_2:1859585-1859604 Msa1226840:intron 40.0%
ACCATGCATGACAAAAGGTT+TGG - chr8_2:1859589-1859608 None:intergenic 40.0%
ATCATAATGCTTACCAGGCA+AGG - chr8_2:1859814-1859833 None:intergenic 40.0%
GGTACATCATAATGCTTACC+AGG - chr8_2:1859819-1859838 None:intergenic 40.0%
GTTGGGACAAAGATAACATC+AGG - chr8_2:1860058-1860077 None:intergenic 40.0%
GGGATGATCATGAGTTACAT+GGG + chr8_2:1859064-1859083 Msa1226840:CDS 40.0%
ATGATCATGAGTTACATGGG+AGG + chr8_2:1859067-1859086 Msa1226840:CDS 40.0%
AGATATCAAGAATGGCAGTG+TGG - chr8_2:1859328-1859347 None:intergenic 40.0%
! ACCAAACCTTTTGTCATGCA+TGG + chr8_2:1859585-1859604 Msa1226840:intron 40.0%
ACCATGCATGACAAAAGGTT+TGG - chr8_2:1859589-1859608 None:intergenic 40.0%
ATCATAATGCTTACCAGGCA+AGG - chr8_2:1859814-1859833 None:intergenic 40.0%
GGTACATCATAATGCTTACC+AGG - chr8_2:1859819-1859838 None:intergenic 40.0%
GTTGGGACAAAGATAACATC+AGG - chr8_2:1860058-1860077 None:intergenic 40.0%
GGGGATGATCATGAGTTACA+TGG + chr8_2:1859063-1859082 Msa1226840:CDS 45.0%
ATGAGTTACATGGGAGGATC+AGG + chr8_2:1859073-1859092 Msa1226840:CDS 45.0%
ATCAAGAATGGCAGTGTGGA+AGG - chr8_2:1859324-1859343 None:intergenic 45.0%
! ACTATTAACATGGCCTTGCC+TGG + chr8_2:1859798-1859817 Msa1226840:CDS 45.0%
GCTATGGTAGCTAAGAGAAC+AGG + chr8_2:1859995-1860014 Msa1226840:CDS 45.0%
GGGGATGATCATGAGTTACA+TGG + chr8_2:1859063-1859082 Msa1226840:CDS 45.0%
ATGAGTTACATGGGAGGATC+AGG + chr8_2:1859073-1859092 Msa1226840:CDS 45.0%
ATCAAGAATGGCAGTGTGGA+AGG - chr8_2:1859324-1859343 None:intergenic 45.0%
! ACTATTAACATGGCCTTGCC+TGG + chr8_2:1859798-1859817 Msa1226840:CDS 45.0%
GCTATGGTAGCTAAGAGAAC+AGG + chr8_2:1859995-1860014 Msa1226840:CDS 45.0%
!!! AAATATGAATATTATTTTAA+AGG + chr8_2:1859479-1859498 Msa1226840:intron 5.0%
!!! AAATATGAATATTATTTTAA+AGG + chr8_2:1859479-1859498 Msa1226840:intron 5.0%
TTAGCTACCATAGCTGCTGG+AGG - chr8_2:1859989-1860008 None:intergenic 50.0%
CTCTTAGCTACCATAGCTGC+TGG - chr8_2:1859992-1860011 None:intergenic 50.0%
TTAGCTACCATAGCTGCTGG+AGG - chr8_2:1859989-1860008 None:intergenic 50.0%
CTCTTAGCTACCATAGCTGC+TGG - chr8_2:1859992-1860011 None:intergenic 50.0%
AGTGGCACCTCCAGCAGCTA+TGG + chr8_2:1859979-1859998 Msa1226840:CDS 60.0%
AGTGGCACCTCCAGCAGCTA+TGG + chr8_2:1859979-1859998 Msa1226840:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 1858972 1860150 1858972 ID=Msa1226840;Name=Msa1226840
chr8_2 mRNA 1858972 1860150 1858972 ID=Msa1226840-mRNA-1;Parent=Msa1226840;Name=Msa1226840-mRNA-1;_AED=0.16;_eAED=0.16;_QI=89|0.66|0.5|1|0.66|0.75|4|0|158
chr8_2 exon 1858972 1859094 1858972 ID=Msa1226840-mRNA-1:exon:334;Parent=Msa1226840-mRNA-1
chr8_2 exon 1859234 1859351 1859234 ID=Msa1226840-mRNA-1:exon:335;Parent=Msa1226840-mRNA-1
chr8_2 exon 1859797 1859819 1859797 ID=Msa1226840-mRNA-1:exon:336;Parent=Msa1226840-mRNA-1
chr8_2 exon 1859849 1860150 1859849 ID=Msa1226840-mRNA-1:exon:337;Parent=Msa1226840-mRNA-1
chr8_2 five_prime_UTR 1858972 1859060 1858972 ID=Msa1226840-mRNA-1:five_prime_utr;Parent=Msa1226840-mRNA-1
chr8_2 CDS 1859061 1859094 1859061 ID=Msa1226840-mRNA-1:cds;Parent=Msa1226840-mRNA-1
chr8_2 CDS 1859234 1859351 1859234 ID=Msa1226840-mRNA-1:cds;Parent=Msa1226840-mRNA-1
chr8_2 CDS 1859797 1859819 1859797 ID=Msa1226840-mRNA-1:cds;Parent=Msa1226840-mRNA-1
chr8_2 CDS 1859849 1860150 1859849 ID=Msa1226840-mRNA-1:cds;Parent=Msa1226840-mRNA-1
Gene Sequence

>Msa1226840

ATGGGGATGATCATGAGTTACATGGGAGGATCAGGTGAGGGAATAGCATCCCAGACATTAGATCTTGTATCAGGAGCACTCTATGATCAATTTATGAAGAAAGAAGTTAAAGACTTTGAAACCTTCCACACTGCCATTCTTGATATCTTCAACACTATTAACATGGCCTTGCCTGAAATTAAGGAAGTTTTCGAAAACTGGAATAAAACATCCGAGGAAGATAAGAAGAAACATATTTTCATTGATTTCATGATCAATAAGGTAAATATAAGCAAAGTAGATGATTCTATGATGATAACTGGAATAGTGGCACCTCCAGCAGCTATGGTAGCTAAGAGAACAGGACAATCTGTGCCTCAACTAAGTGTTATGAAAGCCATTCCTGATGTTATCTTTGTCCCAACAGCTACTATTTTAGCTCTCATTGCTGTTAAAGTCACCAAAAGTATGTCATTGAAGAAATTGAATCCTATTTAA

Protein sequence

>Msa1226840

MGMIMSYMGGSGEGIASQTLDLVSGALYDQFMKKEVKDFETFHTAILDIFNTINMALPEIKEVFENWNKTSEEDKKKHIFIDFMINKVNISKVDDSMMITGIVAPPAAMVAKRTGQSVPQLSVMKAIPDVIFVPTATILALIAVKVTKSMSLKKLNPI*