Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0233320 | XP_003593425.1 | 97.030 | 202 | 6 | 0 | 1 | 202 | 1 | 202 | 1.04e-142 | 405 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0233320 | sp|Q8VZ13|Y1816_ARATH | 35.758 | 165 | 103 | 2 | 41 | 202 | 57 | 221 | 3.53e-33 | 121 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0233320 | G7IK66 | 97.030 | 202 | 6 | 0 | 1 | 202 | 1 | 202 | 4.97e-143 | 405 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002580 | Msa0233320 | 0.802704 | 4.943802e-49 | -8.615850e-47 |
| Msa0005890 | Msa0233320 | 0.807453 | 5.018063e-50 | -8.615850e-47 |
| Msa0029970 | Msa0233320 | 0.800239 | 1.582445e-48 | -8.615850e-47 |
| Msa0034810 | Msa0233320 | 0.817381 | 3.395660e-52 | -8.615850e-47 |
| Msa0071860 | Msa0233320 | 0.811290 | 7.539576e-51 | -8.615850e-47 |
| Msa0071880 | Msa0233320 | 0.824163 | 9.366962e-54 | -8.615850e-47 |
| Msa0081020 | Msa0233320 | 0.800307 | 1.532601e-48 | -8.615850e-47 |
| Msa0081520 | Msa0233320 | 0.804715 | 1.891179e-49 | -8.615850e-47 |
| Msa0102870 | Msa0233320 | 0.804972 | 1.671201e-49 | -8.615850e-47 |
| Msa0129940 | Msa0233320 | 0.820621 | 6.228264e-53 | -8.615850e-47 |
| Msa0164400 | Msa0233320 | 0.808010 | 3.821202e-50 | -8.615850e-47 |
| Msa0174450 | Msa0233320 | 0.806899 | 6.574817e-50 | -8.615850e-47 |
| Msa0175730 | Msa0233320 | 0.837913 | 3.925171e-57 | -8.615850e-47 |
| Msa0186920 | Msa0233320 | 0.853197 | 2.802621e-61 | -8.615850e-47 |
| Msa0191020 | Msa0233320 | 0.997895 | 1.775019e-251 | -8.615850e-47 |
| Msa0194820 | Msa0233320 | 0.812834 | 3.472360e-51 | -8.615850e-47 |
| Msa0194840 | Msa0233320 | 0.812315 | 4.510548e-51 | -8.615850e-47 |
| Msa0211030 | Msa0233320 | 0.824640 | 7.235617e-54 | -8.615850e-47 |
| Msa0225100 | Msa0233320 | 0.826608 | 2.470474e-54 | -8.615850e-47 |
| Msa0225220 | Msa0233320 | 0.805795 | 1.123201e-49 | -8.615850e-47 |
| Msa0225860 | Msa0233320 | 0.827130 | 1.854584e-54 | -8.615850e-47 |
| Msa0233320 | Msa0243600 | 0.811900 | 5.555731e-51 | -8.615850e-47 |
| Msa0233320 | Msa0245280 | 0.808378 | 3.189213e-50 | -8.615850e-47 |
| Msa0233320 | Msa0266180 | 0.824747 | 6.826542e-54 | -8.615850e-47 |
| Msa0233320 | Msa0287120 | 0.818001 | 2.461297e-52 | -8.615850e-47 |
| Msa0233320 | Msa0287740 | 0.830716 | 2.510297e-55 | -8.615850e-47 |
| Msa0233320 | Msa0287780 | 0.816047 | 6.764573e-52 | -8.615850e-47 |
| Msa0233320 | Msa0298470 | 0.820280 | 7.454980e-53 | -8.615850e-47 |
| Msa0233320 | Msa0300270 | 0.804068 | 2.579562e-49 | -8.615850e-47 |
| Msa0233320 | Msa0321620 | 0.811680 | 6.201909e-51 | -8.615850e-47 |
| Msa0233320 | Msa0337700 | 0.801465 | 8.891629e-49 | -8.615850e-47 |
| Msa0233320 | Msa0342010 | 0.807190 | 5.705637e-50 | -8.615850e-47 |
| Msa0233320 | Msa0359020 | 0.812296 | 4.553108e-51 | -8.615850e-47 |
| Msa0233320 | Msa0371170 | 0.839220 | 1.805071e-57 | -8.615850e-47 |
| Msa0233320 | Msa0378720 | 0.808200 | 3.481550e-50 | -8.615850e-47 |
| Msa0233320 | Msa0388770 | 0.806934 | 6.463168e-50 | -8.615850e-47 |
| Msa0233320 | Msa0393150 | 0.820504 | 6.622227e-53 | -8.615850e-47 |
| Msa0233320 | Msa0405680 | 0.812664 | 3.782928e-51 | -8.615850e-47 |
| Msa0233320 | Msa0415610 | 0.816386 | 5.679139e-52 | -8.615850e-47 |
| Msa0233320 | Msa0418970 | 0.825111 | 5.601245e-54 | -8.615850e-47 |
| Msa0233320 | Msa0421890 | 0.804565 | 2.032122e-49 | -8.615850e-47 |
| Msa0233320 | Msa0425170 | 0.821172 | 4.650978e-53 | -8.615850e-47 |
| Msa0233320 | Msa0436910 | 0.802002 | 6.898233e-49 | -8.615850e-47 |
| Msa0233320 | Msa0460630 | 0.812332 | 4.471076e-51 | -8.615850e-47 |
| Msa0233320 | Msa0465400 | 0.831947 | 1.250019e-55 | -8.615850e-47 |
| Msa0233320 | Msa0510480 | 0.814724 | 1.331943e-51 | -8.615850e-47 |
| Msa0233320 | Msa0528570 | 0.823629 | 1.249976e-53 | -8.615850e-47 |
| Msa0233320 | Msa0531180 | 0.814107 | 1.823455e-51 | -8.615850e-47 |
| Msa0233320 | Msa0557460 | 0.807437 | 5.057927e-50 | -8.615850e-47 |
| Msa0233320 | Msa0562620 | 0.820033 | 8.493410e-53 | -8.615850e-47 |
| Msa0233320 | Msa0565660 | 0.815603 | 8.493060e-52 | -8.615850e-47 |
| Msa0233320 | Msa0580370 | 0.820485 | 6.690695e-53 | -8.615850e-47 |
| Msa0233320 | Msa0583360 | -0.801972 | 6.995647e-49 | -8.615850e-47 |
| Msa0233320 | Msa0600930 | 0.805918 | 1.058558e-49 | -8.615850e-47 |
| Msa0233320 | Msa0611210 | 0.803481 | 3.415755e-49 | -8.615850e-47 |
| Msa0233320 | Msa0616510 | 0.818291 | 2.116740e-52 | -8.615850e-47 |
| Msa0233320 | Msa0626450 | 0.814487 | 1.502591e-51 | -8.615850e-47 |
| Msa0233320 | Msa0626490 | 0.808237 | 3.419065e-50 | -8.615850e-47 |
| Msa0233320 | Msa0664600 | 0.809113 | 2.221180e-50 | -8.615850e-47 |
| Msa0233320 | Msa0699840 | 0.822686 | 2.074139e-53 | -8.615850e-47 |
| Msa0233320 | Msa0701700 | 0.813800 | 2.130560e-51 | -8.615850e-47 |
| Msa0233320 | Msa0741220 | 0.847906 | 8.598623e-60 | -8.615850e-47 |
| Msa0233320 | Msa0741650 | 0.803882 | 2.819966e-49 | -8.615850e-47 |
| Msa0233320 | Msa0780270 | 0.813383 | 2.631854e-51 | -8.615850e-47 |
| Msa0233320 | Msa0795870 | 0.819229 | 1.295720e-52 | -8.615850e-47 |
| Msa0233320 | Msa0796160 | 0.809665 | 1.691456e-50 | -8.615850e-47 |
| Msa0233320 | Msa0823820 | 0.804036 | 2.619513e-49 | -8.615850e-47 |
| Msa0233320 | Msa0829300 | 0.831890 | 1.291849e-55 | -8.615850e-47 |
| Msa0233320 | Msa0829330 | 0.818652 | 1.753303e-52 | -8.615850e-47 |
| Msa0233320 | Msa0833650 | 0.820166 | 7.918998e-53 | -8.615850e-47 |
| Msa0233320 | Msa0833760 | 0.800636 | 1.313199e-48 | -8.615850e-47 |
| Msa0233320 | Msa0863010 | 0.803131 | 4.035693e-49 | -8.615850e-47 |
| Msa0233320 | Msa0896440 | 0.819749 | 9.864587e-53 | -8.615850e-47 |
| Msa0233320 | Msa0905240 | 0.806884 | 6.622487e-50 | -8.615850e-47 |
| Msa0233320 | Msa0922980 | 0.803798 | 2.934809e-49 | -8.615850e-47 |
| Msa0233320 | Msa0923030 | 0.810359 | 1.198938e-50 | -8.615850e-47 |
| Msa0233320 | Msa0937230 | 0.800252 | 1.572253e-48 | -8.615850e-47 |
| Msa0233320 | Msa0965290 | 0.809497 | 1.837339e-50 | -8.615850e-47 |
| Msa0233320 | Msa0967870 | 0.818789 | 1.631500e-52 | -8.615850e-47 |
| Msa0233320 | Msa0996330 | 0.804782 | 1.831382e-49 | -8.615850e-47 |
| Msa0233320 | Msa1020630 | 0.805546 | 1.267045e-49 | -8.615850e-47 |
| Msa0233320 | Msa1032780 | 0.808339 | 3.251660e-50 | -8.615850e-47 |
| Msa0233320 | Msa1106510 | 0.813038 | 3.132404e-51 | -8.615850e-47 |
| Msa0233320 | Msa1113800 | 0.824294 | 8.724800e-54 | -8.615850e-47 |
| Msa0233320 | Msa1132700 | 0.802906 | 4.492284e-49 | -8.615850e-47 |
| Msa0233320 | Msa1132840 | 0.822932 | 1.818232e-53 | -8.615850e-47 |
| Msa0233320 | Msa1150820 | 0.800142 | 1.656024e-48 | -8.615850e-47 |
| Msa0233320 | Msa1155170 | -0.818711 | 1.699890e-52 | -8.615850e-47 |
| Msa0233320 | Msa1160500 | 0.826562 | 2.534462e-54 | -8.615850e-47 |
| Msa0233320 | Msa1161810 | 0.810431 | 1.156721e-50 | -8.615850e-47 |
| Msa0233320 | Msa1225860 | -0.808490 | 3.019318e-50 | -8.615850e-47 |
| Msa0233320 | Msa1226840 | 0.811523 | 6.709006e-51 | -8.615850e-47 |
| Msa0233320 | Msa1242370 | 0.805510 | 1.289197e-49 | -8.615850e-47 |
| Msa0233320 | Msa1263490 | 0.801209 | 1.002926e-48 | -8.615850e-47 |
| Msa0233320 | Msa1265650 | 0.803594 | 3.235248e-49 | -8.615850e-47 |
| Msa0233320 | Msa1341890 | 0.824916 | 6.228869e-54 | -8.615850e-47 |
| Msa0233320 | Msa1414640 | 0.805038 | 1.618607e-49 | -8.615850e-47 |
| Msa0233320 | Msa1415540 | 0.813419 | 2.584473e-51 | -8.615850e-47 |
| Msa0233320 | Msa1433810 | 0.851318 | 9.594348e-61 | -8.615850e-47 |
| Msa0233320 | Msa1440960 | 0.804320 | 2.285760e-49 | -8.615850e-47 |
| Msa0233320 | Msa1450100 | 0.811517 | 6.729780e-51 | -8.615850e-47 |
| Msa0233320 | Msa1463900 | 0.802967 | 4.362527e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0233320 | MtrunA17_Chr2g0280851 | 97.030 | 202 | 6 | 0 | 1 | 202 | 1 | 202 | 9.58e-147 | 405 |
| Msa0233320 | MtrunA17_Chr7g0267281 | 30.952 | 210 | 131 | 7 | 4 | 202 | 20 | 226 | 6.45e-25 | 97.1 |
| Msa0233320 | MtrunA17_Chr3g0109121 | 34.965 | 143 | 86 | 3 | 43 | 181 | 88 | 227 | 2.61e-23 | 93.6 |
| Msa0233320 | MtrunA17_Chr1g0177471 | 32.867 | 143 | 95 | 1 | 40 | 181 | 73 | 215 | 5.43e-23 | 92.8 |
| Msa0233320 | MtrunA17_Chr7g0267321 | 32.022 | 178 | 115 | 4 | 14 | 188 | 162 | 336 | 7.26e-23 | 94.4 |
| Msa0233320 | MtrunA17_Chr4g0067041 | 31.915 | 141 | 95 | 1 | 34 | 173 | 64 | 204 | 9.71e-23 | 92.0 |
| Msa0233320 | MtrunA17_Chr5g0440571 | 31.515 | 165 | 108 | 3 | 20 | 180 | 67 | 230 | 1.21e-21 | 89.4 |
| Msa0233320 | MtrunA17_Chr7g0276371 | 28.070 | 171 | 119 | 3 | 22 | 190 | 44 | 212 | 3.72e-21 | 87.4 |
| Msa0233320 | MtrunA17_Chr7g0267181 | 33.690 | 187 | 113 | 4 | 4 | 181 | 18 | 202 | 1.83e-20 | 85.5 |
| Msa0233320 | MtrunA17_Chr7g0267371 | 29.834 | 181 | 118 | 4 | 11 | 183 | 3 | 182 | 1.94e-20 | 84.7 |
| Msa0233320 | MtrunA17_Chr3g0118371 | 28.846 | 156 | 109 | 2 | 27 | 180 | 73 | 228 | 1.62e-19 | 83.6 |
| Msa0233320 | MtrunA17_Chr7g0267361 | 31.361 | 169 | 110 | 4 | 18 | 183 | 68 | 233 | 2.26e-19 | 83.2 |
| Msa0233320 | MtrunA17_Chr5g0433201 | 33.333 | 153 | 101 | 1 | 41 | 192 | 97 | 249 | 2.26e-19 | 83.2 |
| Msa0233320 | MtrunA17_Chr2g0321801 | 26.344 | 186 | 122 | 4 | 20 | 192 | 15 | 198 | 1.05e-18 | 80.5 |
| Msa0233320 | MtrunA17_Chr2g0281401 | 28.022 | 182 | 123 | 5 | 23 | 199 | 80 | 258 | 2.19e-18 | 80.5 |
| Msa0233320 | MtrunA17_Chr7g0267291 | 30.319 | 188 | 121 | 5 | 2 | 180 | 13 | 199 | 9.19e-18 | 79.7 |
| Msa0233320 | MtrunA17_Chr1g0188801 | 31.902 | 163 | 107 | 3 | 43 | 202 | 87 | 248 | 5.48e-17 | 76.6 |
| Msa0233320 | MtrunA17_Chr6g0478671 | 33.663 | 101 | 65 | 2 | 22 | 122 | 9 | 107 | 1.04e-16 | 75.1 |
| Msa0233320 | MtrunA17_Chr6g0479081 | 29.747 | 158 | 106 | 3 | 28 | 180 | 15 | 172 | 1.36e-16 | 74.7 |
| Msa0233320 | MtrunA17_Chr1g0177571 | 29.240 | 171 | 114 | 4 | 14 | 180 | 10 | 177 | 1.46e-16 | 74.7 |
| Msa0233320 | MtrunA17_Chr4g0025681 | 25.773 | 194 | 138 | 4 | 1 | 191 | 3 | 193 | 1.76e-16 | 74.7 |
| Msa0233320 | MtrunA17_Chr3g0127951 | 27.143 | 140 | 100 | 2 | 47 | 185 | 49 | 187 | 4.87e-16 | 73.2 |
| Msa0233320 | MtrunA17_Chr2g0321831 | 26.500 | 200 | 139 | 5 | 3 | 195 | 4 | 202 | 5.17e-16 | 73.2 |
| Msa0233320 | MtrunA17_Chr7g0267301 | 28.261 | 184 | 123 | 4 | 5 | 180 | 21 | 203 | 4.41e-15 | 71.2 |
| Msa0233320 | MtrunA17_Chr6g0479071 | 31.579 | 95 | 65 | 0 | 28 | 122 | 15 | 109 | 5.93e-15 | 70.5 |
| Msa0233320 | MtrunA17_Chr3g0118391 | 26.429 | 140 | 101 | 2 | 43 | 181 | 45 | 183 | 4.54e-14 | 68.2 |
| Msa0233320 | MtrunA17_Chr2g0330241 | 24.000 | 175 | 125 | 3 | 36 | 202 | 77 | 251 | 7.77e-13 | 65.5 |
| Msa0233320 | MtrunA17_Chr3g0115651 | 25.786 | 159 | 110 | 2 | 23 | 180 | 70 | 221 | 1.01e-12 | 65.1 |
| Msa0233320 | MtrunA17_Chr7g0267231 | 25.000 | 160 | 114 | 3 | 13 | 167 | 31 | 189 | 2.82e-12 | 63.5 |
| Msa0233320 | MtrunA17_Chr4g0025861 | 23.226 | 155 | 109 | 3 | 47 | 192 | 44 | 197 | 2.47e-11 | 60.8 |
| Msa0233320 | MtrunA17_Chr3g0118381 | 31.481 | 108 | 72 | 2 | 75 | 180 | 14 | 121 | 2.82e-11 | 59.3 |
| Msa0233320 | MtrunA17_Chr5g0447101 | 21.739 | 161 | 117 | 3 | 11 | 168 | 50 | 204 | 9.07e-11 | 59.3 |
| Msa0233320 | MtrunA17_Chr6g0478661 | 31.313 | 99 | 67 | 1 | 24 | 122 | 7 | 104 | 9.87e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0233320 | AT5G22870.1 | 42.647 | 204 | 113 | 1 | 3 | 202 | 4 | 207 | 4.53e-60 | 187 |
| Msa0233320 | AT1G08160.1 | 35.758 | 165 | 103 | 2 | 41 | 202 | 57 | 221 | 3.59e-34 | 121 |
| Msa0233320 | AT5G06320.1 | 33.511 | 188 | 120 | 3 | 20 | 202 | 44 | 231 | 9.27e-33 | 118 |
| Msa0233320 | AT2G35980.1 | 35.542 | 166 | 105 | 2 | 17 | 180 | 34 | 199 | 8.13e-32 | 115 |
| Msa0233320 | AT3G11650.1 | 30.208 | 192 | 128 | 2 | 17 | 202 | 48 | 239 | 9.69e-31 | 113 |
| Msa0233320 | AT3G52470.1 | 36.145 | 166 | 99 | 4 | 19 | 180 | 14 | 176 | 9.10e-26 | 99.4 |
| Msa0233320 | AT2G35460.1 | 29.282 | 181 | 125 | 3 | 17 | 195 | 47 | 226 | 4.07e-25 | 98.2 |
| Msa0233320 | AT4G09590.1 | 31.579 | 171 | 114 | 2 | 24 | 192 | 22 | 191 | 2.55e-23 | 92.8 |
| Msa0233320 | AT3G11660.1 | 30.233 | 172 | 116 | 3 | 20 | 188 | 14 | 184 | 6.52e-23 | 92.0 |
| Msa0233320 | AT2G27080.1 | 29.221 | 154 | 108 | 1 | 28 | 180 | 82 | 235 | 2.82e-22 | 91.3 |
| Msa0233320 | AT2G27080.2 | 29.221 | 154 | 108 | 1 | 28 | 180 | 82 | 235 | 2.82e-22 | 91.3 |
| Msa0233320 | AT3G44220.1 | 27.848 | 158 | 113 | 1 | 24 | 180 | 20 | 177 | 1.36e-21 | 88.2 |
| Msa0233320 | AT5G53730.1 | 30.612 | 147 | 99 | 3 | 37 | 180 | 40 | 186 | 6.66e-21 | 86.7 |
| Msa0233320 | AT2G35970.1 | 30.994 | 171 | 115 | 2 | 24 | 192 | 22 | 191 | 1.11e-20 | 85.9 |
| Msa0233320 | AT1G65690.1 | 30.000 | 160 | 111 | 1 | 23 | 181 | 68 | 227 | 1.95e-20 | 86.3 |
| Msa0233320 | AT2G35960.1 | 34.932 | 146 | 88 | 4 | 39 | 180 | 36 | 178 | 4.86e-20 | 84.3 |
| Msa0233320 | AT3G52460.1 | 28.796 | 191 | 127 | 3 | 5 | 187 | 90 | 279 | 4.81e-19 | 83.2 |
| Msa0233320 | AT5G22200.1 | 29.114 | 158 | 111 | 1 | 24 | 180 | 24 | 181 | 1.37e-18 | 80.5 |
| Msa0233320 | AT5G06330.1 | 30.719 | 153 | 102 | 3 | 43 | 192 | 39 | 190 | 3.20e-18 | 79.3 |
| Msa0233320 | AT1G61760.1 | 27.835 | 194 | 120 | 5 | 5 | 180 | 9 | 200 | 3.55e-18 | 79.7 |
| Msa0233320 | AT4G01410.1 | 28.125 | 160 | 114 | 1 | 22 | 180 | 42 | 201 | 5.38e-18 | 79.3 |
| Msa0233320 | AT4G05220.1 | 26.471 | 170 | 121 | 3 | 17 | 184 | 39 | 206 | 1.26e-17 | 78.2 |
| Msa0233320 | AT5G21130.1 | 26.230 | 183 | 134 | 1 | 21 | 202 | 96 | 278 | 1.10e-15 | 73.9 |
| Msa0233320 | AT5G36970.1 | 30.612 | 147 | 101 | 1 | 36 | 181 | 77 | 223 | 3.62e-15 | 72.0 |
| Msa0233320 | AT1G54540.1 | 30.405 | 148 | 99 | 3 | 43 | 188 | 76 | 221 | 4.11e-14 | 68.9 |
| Msa0233320 | AT2G27260.1 | 27.072 | 181 | 123 | 4 | 7 | 186 | 51 | 223 | 1.48e-12 | 64.7 |
Find 31 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATATTGACACTTTGGAATT+TGG | 0.133624 | 2_2:+5665164 | None:intergenic |
| CAGAGCAAGGATAAGGTTTA+AGG | 0.222903 | 2_2:-5664940 | Msa0233320:CDS |
| TATTAAGGCTAAAATTATTA+TGG | 0.226061 | 2_2:+5665340 | None:intergenic |
| CTTAAGCTTGAGAGATCTTC+TGG | 0.228941 | 2_2:-5664987 | Msa0233320:CDS |
| CTCTCAAGCTTAAGATCTTT+TGG | 0.241960 | 2_2:+5664996 | None:intergenic |
| AAACGAAACACCGTACTCTA+AGG | 0.304258 | 2_2:-5664905 | Msa0233320:CDS |
| TAAGCCAACAATGATTATTA+AGG | 0.333073 | 2_2:+5665325 | None:intergenic |
| TTACATTTCAACATCACAAT+AGG | 0.375265 | 2_2:+5664827 | None:intergenic |
| TTAGCCTTAATAATCATTGT+TGG | 0.424677 | 2_2:-5665329 | Msa0233320:CDS |
| GTTGAACTGCATTTGTAGCT+AGG | 0.443513 | 2_2:+5665099 | None:intergenic |
| TGTTCATCAGAGCAAGGATA+AGG | 0.446104 | 2_2:-5664947 | Msa0233320:CDS |
| ATCATAGTATATTGACACTT+TGG | 0.456538 | 2_2:+5665157 | None:intergenic |
| GCTTGAGGATTTGATTGTGT+TGG | 0.466829 | 2_2:+5665401 | None:intergenic |
| TCATAAGTTGGCTAGTTGTA+AGG | 0.471372 | 2_2:-5665295 | Msa0233320:CDS |
| TGAGGATTTGATTGTGTTGG+AGG | 0.474694 | 2_2:+5665404 | None:intergenic |
| CATCAGTTAAATTGAAGTTG+TGG | 0.491299 | 2_2:+5665234 | None:intergenic |
| GCTTAGCTGTACTCATAAGT+TGG | 0.503619 | 2_2:-5665307 | Msa0233320:CDS |
| GCAAGGATAAGGTTTAAGGT+TGG | 0.511168 | 2_2:-5664936 | Msa0233320:CDS |
| TTGAACTGCATTTGTAGCTA+GGG | 0.511309 | 2_2:+5665100 | None:intergenic |
| TTAAGCTTGAGAGATCTTCT+GGG | 0.516407 | 2_2:-5664986 | Msa0233320:CDS |
| CTATGATTCTATTGAAGTGT+CGG | 0.534724 | 2_2:-5665141 | Msa0233320:CDS |
| GGTATCTTGTTCACCTGTGT+TGG | 0.539217 | 2_2:-5664883 | Msa0233320:CDS |
| ATCCTAGTAACATTCTTATG+TGG | 0.542159 | 2_2:+5665065 | None:intergenic |
| AACCACATAAGAATGTTACT+AGG | 0.554394 | 2_2:-5665067 | Msa0233320:CDS |
| TGAAGTGTCGGTGCGTTACG+AGG | 0.582552 | 2_2:-5665129 | Msa0233320:CDS |
| AAGACTCACCGCTCAAACTG+TGG | 0.584343 | 2_2:-5665036 | Msa0233320:CDS |
| AACGAAACACCGTACTCTAA+GGG | 0.584440 | 2_2:-5664904 | Msa0233320:CDS |
| AACAAGATACCCTTAGAGTA+CGG | 0.596628 | 2_2:+5664895 | None:intergenic |
| TAGATGTGTTCATCAGAGCA+AGG | 0.642918 | 2_2:-5664953 | Msa0233320:CDS |
| TAAGCTTGAGAGATCTTCTG+GGG | 0.656491 | 2_2:-5664985 | Msa0233320:CDS |
| ATACAAAGCCACAGTTTGAG+CGG | 0.660862 | 2_2:+5665028 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTAAGGCTAAAATTATTA+TGG | + | chr2_2:5664903-5664922 | None:intergenic | 15.0% |
| !!! | TTAGCCTTAATAATCATTGT+TGG | - | chr2_2:5664911-5664930 | Msa0233320:CDS | 25.0% |
| ! | TAAGCCAACAATGATTATTA+AGG | + | chr2_2:5664918-5664937 | None:intergenic | 25.0% |
| !!! | ACACTATATTGCAAGTTTTT+TGG | + | chr2_2:5664971-5664990 | None:intergenic | 25.0% |
| !!! | TATATTGACACTTTGGAATT+TGG | + | chr2_2:5665079-5665098 | None:intergenic | 25.0% |
| ! | ATCATAGTATATTGACACTT+TGG | + | chr2_2:5665086-5665105 | None:intergenic | 25.0% |
| ! | TTCTTATGTGGTTGAAAAAA+CGG | + | chr2_2:5665166-5665185 | None:intergenic | 25.0% |
| !!! | TAAAGCTAAGAGTTTTGAAA+GGG | - | chr2_2:5665390-5665409 | Msa0233320:CDS | 25.0% |
| ! | TTACATTTCAACATCACAAT+AGG | + | chr2_2:5665416-5665435 | None:intergenic | 25.0% |
| CATCAGTTAAATTGAAGTTG+TGG | + | chr2_2:5665009-5665028 | None:intergenic | 30.0% | |
| CTATGATTCTATTGAAGTGT+CGG | - | chr2_2:5665099-5665118 | Msa0233320:CDS | 30.0% | |
| AACCACATAAGAATGTTACT+AGG | - | chr2_2:5665173-5665192 | Msa0233320:CDS | 30.0% | |
| ATCCTAGTAACATTCTTATG+TGG | + | chr2_2:5665178-5665197 | None:intergenic | 30.0% | |
| TTAGAAAAATTCACCAACAC+AGG | + | chr2_2:5665373-5665392 | None:intergenic | 30.0% | |
| !!! | CTAAAGCTAAGAGTTTTGAA+AGG | - | chr2_2:5665389-5665408 | Msa0233320:CDS | 30.0% |
| !!! | CAGCATTGAACTTTTTGGTT+TGG | + | chr2_2:5664867-5664886 | None:intergenic | 35.0% |
| ! | TCATAAGTTGGCTAGTTGTA+AGG | - | chr2_2:5664945-5664964 | Msa0233320:CDS | 35.0% |
| TTGAACTGCATTTGTAGCTA+GGG | + | chr2_2:5665143-5665162 | None:intergenic | 35.0% | |
| ! | CTCTCAAGCTTAAGATCTTT+TGG | + | chr2_2:5665247-5665266 | None:intergenic | 35.0% |
| TTAAGCTTGAGAGATCTTCT+GGG | - | chr2_2:5665254-5665273 | Msa0233320:CDS | 35.0% | |
| AACAAGATACCCTTAGAGTA+CGG | + | chr2_2:5665348-5665367 | None:intergenic | 35.0% | |
| TGAGGATTTGATTGTGTTGG+AGG | + | chr2_2:5664839-5664858 | None:intergenic | 40.0% | |
| GCTTGAGGATTTGATTGTGT+TGG | + | chr2_2:5664842-5664861 | None:intergenic | 40.0% | |
| !!! | CTTTTTGGTTTGGTTGCTTG+AGG | + | chr2_2:5664857-5664876 | None:intergenic | 40.0% |
| !! | CGTAGCAGCATTGAACTTTT+TGG | + | chr2_2:5664872-5664891 | None:intergenic | 40.0% |
| GCTTAGCTGTACTCATAAGT+TGG | - | chr2_2:5664933-5664952 | Msa0233320:CDS | 40.0% | |
| GTTGAACTGCATTTGTAGCT+AGG | + | chr2_2:5665144-5665163 | None:intergenic | 40.0% | |
| ATACAAAGCCACAGTTTGAG+CGG | + | chr2_2:5665215-5665234 | None:intergenic | 40.0% | |
| CTTAAGCTTGAGAGATCTTC+TGG | - | chr2_2:5665253-5665272 | Msa0233320:CDS | 40.0% | |
| TAAGCTTGAGAGATCTTCTG+GGG | - | chr2_2:5665255-5665274 | Msa0233320:CDS | 40.0% | |
| TAGATGTGTTCATCAGAGCA+AGG | - | chr2_2:5665287-5665306 | Msa0233320:CDS | 40.0% | |
| TGTTCATCAGAGCAAGGATA+AGG | - | chr2_2:5665293-5665312 | Msa0233320:CDS | 40.0% | |
| CAGAGCAAGGATAAGGTTTA+AGG | - | chr2_2:5665300-5665319 | Msa0233320:CDS | 40.0% | |
| ! | GCAAGGATAAGGTTTAAGGT+TGG | - | chr2_2:5665304-5665323 | Msa0233320:CDS | 40.0% |
| AAACGAAACACCGTACTCTA+AGG | - | chr2_2:5665335-5665354 | Msa0233320:CDS | 40.0% | |
| AACGAAACACCGTACTCTAA+GGG | - | chr2_2:5665336-5665355 | Msa0233320:CDS | 40.0% | |
| GGTATCTTGTTCACCTGTGT+TGG | - | chr2_2:5665357-5665376 | Msa0233320:CDS | 45.0% | |
| AAGACTCACCGCTCAAACTG+TGG | - | chr2_2:5665204-5665223 | Msa0233320:CDS | 50.0% | |
| !! | TGAAGTGTCGGTGCGTTACG+AGG | - | chr2_2:5665111-5665130 | Msa0233320:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_2 | gene | 5664827 | 5665435 | 5664827 | ID=Msa0233320;Name=Msa0233320 |
| chr2_2 | mRNA | 5664827 | 5665435 | 5664827 | ID=Msa0233320-mRNA-1;Parent=Msa0233320;Name=Msa0233320-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|202 |
| chr2_2 | exon | 5664827 | 5665435 | 5664827 | ID=Msa0233320-mRNA-1:exon:2429;Parent=Msa0233320-mRNA-1 |
| chr2_2 | CDS | 5664827 | 5665435 | 5664827 | ID=Msa0233320-mRNA-1:cds;Parent=Msa0233320-mRNA-1 |
| Gene Sequence |
| Protein sequence |
