Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1231180 | XP_039684346.1 | 91.030 | 825 | 51 | 2 | 1 | 802 | 1 | 825 | 0.0 | 1532 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1231180 | sp|Q9SXB8|Y1133_ARATH | 46.715 | 822 | 375 | 17 | 32 | 802 | 33 | 842 | 0.0 | 719 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0059890 | Msa1231180 | 0.807673 | 4.505967e-50 | -8.615850e-47 |
| Msa0077180 | Msa1231180 | 0.808335 | 3.257194e-50 | -8.615850e-47 |
| Msa0085720 | Msa1231180 | 0.820474 | 6.730140e-53 | -8.615850e-47 |
| Msa0124550 | Msa1231180 | 0.818533 | 1.865758e-52 | -8.615850e-47 |
| Msa0142280 | Msa1231180 | 0.804100 | 2.539947e-49 | -8.615850e-47 |
| Msa0175050 | Msa1231180 | 0.804270 | 2.341648e-49 | -8.615850e-47 |
| Msa0410580 | Msa1231180 | 0.809609 | 1.739021e-50 | -8.615850e-47 |
| Msa0627350 | Msa1231180 | 0.800932 | 1.142638e-48 | -8.615850e-47 |
| Msa0654620 | Msa1231180 | 0.807994 | 3.851123e-50 | -8.615850e-47 |
| Msa0679550 | Msa1231180 | 0.805430 | 1.340175e-49 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1231180 | MtrunA17_Chr8g0340091 | 84.764 | 827 | 74 | 6 | 1 | 802 | 53 | 852 | 0.0 | 1409 |
| Msa1231180 | MtrunA17_Chr8g0340081 | 83.152 | 825 | 115 | 3 | 1 | 802 | 3 | 826 | 0.0 | 1390 |
| Msa1231180 | MtrunA17_Chr8g0340071 | 82.545 | 825 | 118 | 4 | 1 | 802 | 1 | 822 | 0.0 | 1370 |
| Msa1231180 | MtrunA17_Chr8g0340111 | 80.072 | 828 | 135 | 7 | 1 | 802 | 1 | 824 | 0.0 | 1294 |
| Msa1231180 | MtrunA17_Chr8g0340061 | 92.405 | 474 | 33 | 1 | 1 | 471 | 1 | 474 | 0.0 | 897 |
| Msa1231180 | MtrunA17_Chr2g0280791 | 49.231 | 845 | 371 | 17 | 7 | 802 | 2 | 837 | 0.0 | 810 |
| Msa1231180 | MtrunA17_Chr2g0280801 | 49.881 | 840 | 363 | 14 | 11 | 802 | 14 | 843 | 0.0 | 802 |
| Msa1231180 | MtrunA17_Chr7g0236691 | 49.639 | 830 | 379 | 11 | 1 | 802 | 1 | 819 | 0.0 | 788 |
| Msa1231180 | MtrunA17_Chr7g0236121 | 50.000 | 814 | 371 | 10 | 1 | 786 | 1 | 806 | 0.0 | 781 |
| Msa1231180 | MtrunA17_Chr7g0236551 | 49.204 | 817 | 372 | 10 | 11 | 802 | 9 | 807 | 0.0 | 776 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1231180 | AT1G11330.1 | 46.829 | 820 | 375 | 17 | 32 | 802 | 33 | 840 | 0.0 | 721 |
| Msa1231180 | AT1G11330.2 | 46.715 | 822 | 375 | 17 | 32 | 802 | 33 | 842 | 0.0 | 719 |
| Msa1231180 | AT1G11300.1 | 44.564 | 837 | 404 | 16 | 6 | 802 | 4 | 820 | 0.0 | 702 |
| Msa1231180 | AT1G11300.2 | 44.072 | 835 | 396 | 15 | 6 | 802 | 4 | 805 | 0.0 | 693 |
| Msa1231180 | AT1G11303.1 | 43.914 | 838 | 408 | 18 | 6 | 802 | 4 | 820 | 0.0 | 670 |
| Msa1231180 | AT1G11350.1 | 44.254 | 818 | 401 | 14 | 30 | 802 | 23 | 830 | 0.0 | 658 |
| Msa1231180 | AT4G21390.1 | 42.890 | 865 | 397 | 24 | 11 | 802 | 9 | 849 | 0.0 | 645 |
| Msa1231180 | AT1G61610.1 | 42.142 | 859 | 423 | 19 | 1 | 802 | 1 | 842 | 0.0 | 643 |
| Msa1231180 | AT1G11330.3 | 47.018 | 721 | 319 | 17 | 32 | 701 | 33 | 741 | 0.0 | 629 |
| Msa1231180 | AT1G61430.9 | 40.854 | 820 | 433 | 15 | 13 | 802 | 6 | 803 | 0.0 | 603 |
Find 155 sgRNAs with CRISPR-Local
Find 447 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAACAGTTGATATACT+TGG | 0.549330 | 8_2:+7270515 | None:intergenic |
| AAAAGACTCTCAAAGGCATC+AGG | 0.492026 | 8_2:-7271915 | Msa1231180:CDS |
| AAAATACTATTATTTGATCA+TGG | 0.427891 | 8_2:-7270296 | Msa1231180:three_prime_UTR |
| AAAATTTAGATTGGAGAAAG+CGG | 0.571983 | 8_2:-7271374 | Msa1231180:CDS |
| AAACTATATCTTGTGGATTG+TGG | 0.461269 | 8_2:+7277500 | None:intergenic |
| AAAGATGCATCCCATATCTC+TGG | 0.463319 | 8_2:+7270605 | None:intergenic |
| AAAGGAACTTCTTAGATTTG+AGG | 0.378227 | 8_2:-7276558 | Msa1231180:CDS |
| AAATACTTGGTTAACATACT+CGG | 0.597803 | 8_2:+7276713 | None:intergenic |
| AAATCAGATGTCTATAGCTT+TGG | 0.360740 | 8_2:-7270994 | Msa1231180:CDS |
| AAATGTTCTGCTAGGCATAA+AGG | 0.410249 | 8_2:-7275754 | Msa1231180:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATTACAAGGATTGTTGCTA+GGG | + | chr8_2:7270202-7270221 | None:intergenic | 30.0% | |
| CCTATTCAAACTATATCTTG+TGG | + | chr8_2:7270312-7270331 | None:intergenic | 30.0% | |
| CCACAAGATATAGTTTGAAT+AGG | - | chr8_2:7270309-7270328 | Msa1231180:three_prime_UTR | 30.0% | |
| AGGAATACTAGAACTTAGTT+TGG | + | chr8_2:7270364-7270383 | None:intergenic | 30.0% | |
| TGTACCAAATTCCTAAGTAA+CGG | + | chr8_2:7270614-7270633 | None:intergenic | 30.0% | |
| ATTCCATTTGAATCTTTGAG+TGG | + | chr8_2:7270678-7270697 | None:intergenic | 30.0% | |
| ATTGTCACAATTCAGAAAGA+TGG | - | chr8_2:7270696-7270715 | Msa1231180:intron | 30.0% | |
| GTCATATTGAACAAACCAAA+TGG | - | chr8_2:7270726-7270745 | Msa1231180:intron | 30.0% | |
| GACCAAATGATAATACCATT+TGG | + | chr8_2:7270744-7270763 | None:intergenic | 30.0% | |
| TTAGCTTCAATTCTCATTGT+TGG | + | chr8_2:7270894-7270913 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 7270133 | 7277691 | 7270133 | ID=Msa1231180;Name=Msa1231180 |
| chr8_2 | mRNA | 7270133 | 7277691 | 7270133 | ID=Msa1231180-mRNA-1;Parent=Msa1231180;Name=Msa1231180-mRNA-1;_AED=0.03;_eAED=0.03;_QI=290|0.83|0.85|1|1|1|7|231|802 |
| chr8_2 | exon | 7270133 | 7270672 | 7270133 | ID=Msa1231180-mRNA-1:exon:2713;Parent=Msa1231180-mRNA-1 |
| chr8_2 | exon | 7270912 | 7271062 | 7270912 | ID=Msa1231180-mRNA-1:exon:2712;Parent=Msa1231180-mRNA-1 |
| chr8_2 | exon | 7271237 | 7271411 | 7271237 | ID=Msa1231180-mRNA-1:exon:2711;Parent=Msa1231180-mRNA-1 |
| chr8_2 | exon | 7271760 | 7271970 | 7271760 | ID=Msa1231180-mRNA-1:exon:2710;Parent=Msa1231180-mRNA-1 |
| chr8_2 | exon | 7272054 | 7272205 | 7272054 | ID=Msa1231180-mRNA-1:exon:2709;Parent=Msa1231180-mRNA-1 |
| chr8_2 | exon | 7275755 | 7275883 | 7275755 | ID=Msa1231180-mRNA-1:exon:2708;Parent=Msa1231180-mRNA-1 |
| chr8_2 | exon | 7276120 | 7277691 | 7276120 | ID=Msa1231180-mRNA-1:exon:2707;Parent=Msa1231180-mRNA-1 |
| chr8_2 | five_prime_UTR | 7277402 | 7277691 | 7277402 | ID=Msa1231180-mRNA-1:five_prime_utr;Parent=Msa1231180-mRNA-1 |
| chr8_2 | CDS | 7276120 | 7277401 | 7276120 | ID=Msa1231180-mRNA-1:cds;Parent=Msa1231180-mRNA-1 |
| chr8_2 | CDS | 7275755 | 7275883 | 7275755 | ID=Msa1231180-mRNA-1:cds;Parent=Msa1231180-mRNA-1 |
| chr8_2 | CDS | 7272054 | 7272205 | 7272054 | ID=Msa1231180-mRNA-1:cds;Parent=Msa1231180-mRNA-1 |
| chr8_2 | CDS | 7271760 | 7271970 | 7271760 | ID=Msa1231180-mRNA-1:cds;Parent=Msa1231180-mRNA-1 |
| chr8_2 | CDS | 7271237 | 7271411 | 7271237 | ID=Msa1231180-mRNA-1:cds;Parent=Msa1231180-mRNA-1 |
| chr8_2 | CDS | 7270912 | 7271062 | 7270912 | ID=Msa1231180-mRNA-1:cds;Parent=Msa1231180-mRNA-1 |
| chr8_2 | CDS | 7270364 | 7270672 | 7270364 | ID=Msa1231180-mRNA-1:cds;Parent=Msa1231180-mRNA-1 |
| chr8_2 | three_prime_UTR | 7270133 | 7270363 | 7270133 | ID=Msa1231180-mRNA-1:three_prime_utr;Parent=Msa1231180-mRNA-1 |
| Gene Sequence |
>Msa1231180 ATGGCTTTTCTAAGTCATAATACAAATGACTTATTCATTACATTCCTCATATTTTGCACATTTTATTCATGCTATAGTTCTACCAATAACGCCATTACATCATCAAAATCACTCAAAGACCAAGAGACAATAACCTCAAACAACACAAACTTCAAGCTAGGATTTTTCAGCCCATTGAATTCAACCAACCGTTACTTAGGAATTTGGTACATCAATGAGACCAACAACATATGGATTGCAAACAGAGATCAACCACTCAAAGATTCAAATGGAATTGTCACAATTCAGAAAGATGGAAATCTTGTCATATTGAACAAACCAAATGGTATTATCATTTGGTCAACAAACATTTCAACATCAACAAATTCAACAGCTAAACTTGATGATGCAGGGAACTTGATTCTAAGAGACATCAATTCTGGAGCAACAATATGGGATAGTTTCACACACCCTGCCGATGCAGCTGTGCCAACAATGAGAATTGAAGCTAACCAAGTAACAGGTAAGAAAATATCCTTTGTATCAAGAAAAAGTGAAAATGATCCTTCTTCAGGACATTATTCAGCTAGTCTTGAAAGATTGGATGCACCAGAAGTTTTTATTTGGTATGATAAGAAAATTCATTGGAGAACTGGTCCATGGAATGGACTGGTTTTTCTCGGAACACCGAGTATGTTAACCAAGTATTTACACGGTTGGCGTTTTGATCAAGACAGTGATGGAACTACTTATATTACTTACAATTTCGCCGACAAAACTATGTTTGGAATTCTCTCATTGACACCACATGGAACACTTAAGTTGGTTGAGTATATGAATAAAAAGGAACTTCTTAGATTTGAGGTTGATCAAAATGAGTGTGATTTTTATGGAAAATGCGGGCCATTTGGTAACTGTGATAATTCTATTGTGCCAATTTGTAGTTGTTTTGATGGGTTTGAGCCAAAGAATTCTGTGGAATGGAGTATAGGAAATTGGACCAATGGTTGTGTGAGGAAAGAGGGAATGAACTTGAAGTGTGAGATGGTGAAGAATGGATCAAATCTAGTGAAACAAGATGGATTTAAGGTGTATCATAATATGAAAGTTCCTGATTTTGCTGAGAGATCAACTGCTGATATAGATATGTGTGGAGAAGATTGTTTGGCAAATTGTTCTTGTTTGGCATATGCATATGATCCTTCTATATTTTGTATGTATTGGACTGTAGAGTTAATTGATTTGCAGAAATTTCCTTATGGTGGAGTTGATCTCTTCATTCGAGTGCCGGGCGCAGAACTTGTTGCTGTTAAAAAGGAAAAAGGACACAGAAAGAGTTTTTTAATCATTGTAATTGCTGGGGTGATAGGAGCACTTATCTTGGTTATATGTGCTTATCTTTTATGGAGGAAATGTTCTGCTAGGCATAAAGGAAGACTACCTCAAAATATGATTACCAGAGAACATAAACAAATGAAACTGGATGAGCTACCACTATATGATTTTGAAAAGCTTGAAACTGCCACAAACAGTTTTCATTTTAATAATATGCTTGGGAAGGGAGGTTTTGGCCCAGTATACAAGGGAGTCATGGAAGATGGTCAAGAAATTGCCGTGAAAAGACTCTCAAAGGCATCAGGACAAGGAATAGAAGAATTCATGAACGAAGTAGTAGTAATTTCTAAACTTCAACATCGCAATCTTGTAAGACTTCTAGGTTGTTGTGTTGAAAGGGGAGAGCAAATGTTGGTTTATGAGTTCATGCCAAATAAAAGTTTGGATATCTTTATCTTTGATCCATTACAAAAGAAAAATTTAGATTGGAGAAAGCGGTGGAACATAGTTGAAGGAATAGCTCGAGGTATAATGTATCTTCATAGAGACTCAAGACTTAAGATTATACATAGAGATTTAAAAGCAAGCAATGTCTTACTTGATGGAGACATGATTCCAAAGATATCAGACTTTGGTGGTTATATGCCACCTGAATATGCAATGGAGGGGCTTTTCTCTGAGAAATCAGATGTCTATAGCTTTGGGGTTTTATTGCTAGAGATTGTCAGTGGGAGAAGAAATACGAGCTTTTATCACAGCGAGGATTCTCTTAGCCTTGTAGGCTTTGCATGGAAGCTATGGTTAGAAGAAAACATTATATCACTAATAGATCCAGAGATATGGGATGCATCTTTCGAGAGCAGCATGCTTAGGTGCATACACATAGGACTTCTTTGTGTGCAAGAACTTCCAAAAGAAAGACCAAGTATATCAACTGTTGTTTTGATGCTCATAAATGAGATCAGACATCTTCCAACTCCAGGTAAAGTTGCATTTGTTCACAAGCAAAATTTTCGAAGTACAGAATCTTCTCAAAAAAGGCATCAATTTAACTCAAACAACAATGTAACTTTGAGTGATGTCACAGGAAGGTAA |
| Protein sequence |
>Msa1231180 MAFLSHNTNDLFITFLIFCTFYSCYSSTNNAITSSKSLKDQETITSNNTNFKLGFFSPLNSTNRYLGIWYINETNNIWIANRDQPLKDSNGIVTIQKDGNLVILNKPNGIIIWSTNISTSTNSTAKLDDAGNLILRDINSGATIWDSFTHPADAAVPTMRIEANQVTGKKISFVSRKSENDPSSGHYSASLERLDAPEVFIWYDKKIHWRTGPWNGLVFLGTPSMLTKYLHGWRFDQDSDGTTYITYNFADKTMFGILSLTPHGTLKLVEYMNKKELLRFEVDQNECDFYGKCGPFGNCDNSIVPICSCFDGFEPKNSVEWSIGNWTNGCVRKEGMNLKCEMVKNGSNLVKQDGFKVYHNMKVPDFAERSTADIDMCGEDCLANCSCLAYAYDPSIFCMYWTVELIDLQKFPYGGVDLFIRVPGAELVAVKKEKGHRKSFLIIVIAGVIGALILVICAYLLWRKCSARHKGRLPQNMITREHKQMKLDELPLYDFEKLETATNSFHFNNMLGKGGFGPVYKGVMEDGQEIAVKRLSKASGQGIEEFMNEVVVISKLQHRNLVRLLGCCVERGEQMLVYEFMPNKSLDIFIFDPLQKKNLDWRKRWNIVEGIARGIMYLHRDSRLKIIHRDLKASNVLLDGDMIPKISDFGGYMPPEYAMEGLFSEKSDVYSFGVLLLEIVSGRRNTSFYHSEDSLSLVGFAWKLWLEENIISLIDPEIWDASFESSMLRCIHIGLLCVQELPKERPSISTVVLMLINEIRHLPTPGKVAFVHKQNFRSTESSQKRHQFNSNNNVTLSDVTGR* |
