AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0142280


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0142280 MtrunA17_Chr4g0076471 74.091 220 27 5 1 193 7 223 5.81e-101 294
Msa0142280 MtrunA17_Chr4g0076451 37.405 131 50 5 93 193 50 178 1.02e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0142280 AT5G47740.2 37.821 156 63 5 63 192 21 168 4.80e-22 90.1
Msa0142280 AT5G47740.1 37.662 154 64 5 63 192 21 166 1.04e-21 89.4

Find 40 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGTGTTTGATTACAATTT+CGG 0.312597 1_4:-1196892 Msa0142280:CDS
GAAACTAAATATAAGGAAAT+TGG 0.325459 1_4:-1195911 Msa0142280:CDS
GAACTACCCAATCTAATCTA+AGG 0.351629 1_4:-1195882 None:intergenic
TTTGAAGAAGGAAAGTAGAT+TGG 0.355768 1_4:-1196827 Msa0142280:CDS
TTGTAGGGAAACTAAATATA+AGG 0.364114 1_4:-1195918 Msa0142280:CDS
TATGTTACCTTAGATTAGAT+TGG 0.375634 1_4:+1195875 None:intergenic
CAAGCATGGAAGCACTTTCA+TGG 0.384069 1_4:-1196684 Msa0142280:CDS
AATAAAACCACAACCACAAC+TGG 0.394819 1_4:+1196930 None:intergenic
TCTTGAATATCATGCTTAGT+TGG 0.411358 1_4:+1196988 None:intergenic
TTGGAATTAGTTTGACTTGA+GGG 0.414606 1_4:+1196605 None:intergenic
AGAGAAACAGTAGTTGTAGA+AGG 0.431084 1_4:+1196640 None:intergenic
AAAGCGTAACATACAGGTTA+AGG 0.436501 1_4:-1195992 Msa0142280:CDS
GAAATCTCCAGTTGTGGTTG+TGG 0.445667 1_4:-1196937 Msa0142280:CDS
GGTAGAAATGATGCTTATTG+AGG 0.452714 1_4:-1195971 Msa0142280:CDS
GAAGCCATTGTGGAACTTGT+AGG 0.460657 1_4:-1195934 Msa0142280:CDS
GTTTGAGATAAATTTGAAGA+AGG 0.464932 1_4:-1196839 Msa0142280:CDS
AATATAAGGAAATTGGTGAT+TGG 0.465583 1_4:-1195904 Msa0142280:CDS
ATGTTACCTTAGATTAGATT+GGG 0.474526 1_4:+1195876 None:intergenic
AGGTGTCACGGCATGTTTCA+AGG 0.477649 1_4:+1196660 None:intergenic
GTAGAAATGATGCTTATTGA+GGG 0.483302 1_4:-1195970 Msa0142280:CDS
TATAGATGATGTTGTTTATG+TGG 0.505857 1_4:-1196731 Msa0142280:CDS
TAATGAAAAGCGTAACATAC+AGG 0.506176 1_4:-1195998 Msa0142280:CDS
AATTTCGGAGACGACGATGA+TGG 0.516786 1_4:-1196877 Msa0142280:CDS
AAGGAGTAGTTACATGGAGT+TGG 0.519333 1_4:+1196586 None:intergenic
CGAAATTGTAATCAAACACT+TGG 0.523720 1_4:+1196893 None:intergenic
ATTGGATTCAATAAGAGAAG+AGG 0.530259 1_4:-1196809 Msa0142280:CDS
AAGCCATTGTGGAACTTGTA+GGG 0.539241 1_4:-1195933 Msa0142280:CDS
TATGTGGCAGTGGGAACAAA+TGG 0.556548 1_4:-1196715 Msa0142280:CDS
TGATGTTGTTTATGTGGCAG+TGG 0.557388 1_4:-1196725 Msa0142280:CDS
TAAGACAAGGAGTAGTTACA+TGG 0.583206 1_4:+1196580 None:intergenic
CAACGTTGCTGAAGCCATTG+TGG 0.587024 1_4:-1195944 Msa0142280:CDS
GTTGGAATTAGTTTGACTTG+AGG 0.589120 1_4:+1196604 None:intergenic
TGACTTGAGGGAAAACGTGT+AGG 0.604542 1_4:+1196617 None:intergenic
AAATGGTGGAAGTTCAAGCA+TGG 0.604604 1_4:-1196698 Msa0142280:CDS
TTTCCCTACAAGTTCCACAA+TGG 0.638145 1_4:+1195930 None:intergenic
GATGTTGTTTATGTGGCAGT+GGG 0.642705 1_4:-1196724 Msa0142280:CDS
AGTAGTTGTAGAAGGTGTCA+CGG 0.650946 1_4:+1196648 None:intergenic
ATTCAATAAGAGAAGAGGAG+TGG 0.652515 1_4:-1196804 Msa0142280:CDS
GTGGCAGTGGGAACAAATGG+TGG 0.662195 1_4:-1196712 Msa0142280:CDS
GATGATGAAATCTCCAGTTG+TGG 0.697511 1_4:-1196943 Msa0142280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTAGATAAAGTTAAATT+TGG + chr1_4:1196377-1196396 None:intergenic 15.0%
!!! TGACAATAATATGTTTTTTT+TGG - chr1_4:1196558-1196577 Msa0142280:intron 15.0%
!!! AAAACTTATTTTGAATGTTA+TGG + chr1_4:1196631-1196650 None:intergenic 15.0%
!! CAATTAGATAAAGTTAAATT+TGG + chr1_4:1196377-1196396 None:intergenic 15.0%
!!! TGACAATAATATGTTTTTTT+TGG - chr1_4:1196558-1196577 Msa0142280:intron 15.0%
!!! AAAACTTATTTTGAATGTTA+TGG + chr1_4:1196631-1196650 None:intergenic 15.0%
!!! TTTTGTTTGAAAATAAGACA+AGG + chr1_4:1196315-1196334 None:intergenic 20.0%
!!! AATGTTATGGTTATGTTTTT+GGG + chr1_4:1196618-1196637 None:intergenic 20.0%
!! TTTCATAATCTTAAACAACT+TGG - chr1_4:1196660-1196679 Msa0142280:CDS 20.0%
!! AAACCTTATATATGTATTGA+TGG - chr1_4:1196833-1196852 Msa0142280:CDS 20.0%
!! GAAACTAAATATAAGGAAAT+TGG - chr1_4:1196968-1196987 Msa0142280:CDS 20.0%
!!! TTTTGTTTGAAAATAAGACA+AGG + chr1_4:1196315-1196334 None:intergenic 20.0%
!!! AATGTTATGGTTATGTTTTT+GGG + chr1_4:1196618-1196637 None:intergenic 20.0%
!! TTTCATAATCTTAAACAACT+TGG - chr1_4:1196660-1196679 Msa0142280:CDS 20.0%
!! AAACCTTATATATGTATTGA+TGG - chr1_4:1196833-1196852 Msa0142280:CDS 20.0%
!! GAAACTAAATATAAGGAAAT+TGG - chr1_4:1196968-1196987 Msa0142280:CDS 20.0%
! CAAGTGTTTGATTACAATTT+CGG - chr1_4:1195987-1196006 Msa0142280:CDS 25.0%
!! GTTTGAGATAAATTTGAAGA+AGG - chr1_4:1196040-1196059 Msa0142280:intron 25.0%
!! TATAGATGATGTTGTTTATG+TGG - chr1_4:1196148-1196167 Msa0142280:intron 25.0%
! TATGATGTGCAATTAATAAG+AGG + chr1_4:1196417-1196436 None:intergenic 25.0%
!! AAAGATTTGATCACATCTTT+TGG + chr1_4:1196506-1196525 None:intergenic 25.0%
!! GAATGTTATGGTTATGTTTT+TGG + chr1_4:1196619-1196638 None:intergenic 25.0%
! GAAGCTATATAGTATGATTT+AGG + chr1_4:1196704-1196723 None:intergenic 25.0%
! ACTCCATCAATACATATATA+AGG + chr1_4:1196839-1196858 None:intergenic 25.0%
! TTGTAGGGAAACTAAATATA+AGG - chr1_4:1196961-1196980 Msa0142280:CDS 25.0%
!! AATATAAGGAAATTGGTGAT+TGG - chr1_4:1196975-1196994 Msa0142280:CDS 25.0%
! CAAGTGTTTGATTACAATTT+CGG - chr1_4:1195987-1196006 Msa0142280:CDS 25.0%
!! GTTTGAGATAAATTTGAAGA+AGG - chr1_4:1196040-1196059 Msa0142280:intron 25.0%
!! TATAGATGATGTTGTTTATG+TGG - chr1_4:1196148-1196167 Msa0142280:intron 25.0%
! TATGATGTGCAATTAATAAG+AGG + chr1_4:1196417-1196436 None:intergenic 25.0%
!! AAAGATTTGATCACATCTTT+TGG + chr1_4:1196506-1196525 None:intergenic 25.0%
!! GAATGTTATGGTTATGTTTT+TGG + chr1_4:1196619-1196638 None:intergenic 25.0%
! GAAGCTATATAGTATGATTT+AGG + chr1_4:1196704-1196723 None:intergenic 25.0%
! ACTCCATCAATACATATATA+AGG + chr1_4:1196839-1196858 None:intergenic 25.0%
! TTGTAGGGAAACTAAATATA+AGG - chr1_4:1196961-1196980 Msa0142280:CDS 25.0%
!! AATATAAGGAAATTGGTGAT+TGG - chr1_4:1196975-1196994 Msa0142280:CDS 25.0%
TCTTGAATATCATGCTTAGT+TGG + chr1_4:1195894-1195913 None:intergenic 30.0%
CGAAATTGTAATCAAACACT+TGG + chr1_4:1195989-1196008 None:intergenic 30.0%
TTTGAAGAAGGAAAGTAGAT+TGG - chr1_4:1196052-1196071 Msa0142280:intron 30.0%
ATTGGATTCAATAAGAGAAG+AGG - chr1_4:1196070-1196089 Msa0142280:intron 30.0%
!! TTGGAATTAGTTTGACTTGA+GGG + chr1_4:1196277-1196296 None:intergenic 30.0%
! TTGGTGATTTATGCTTATGT+TGG + chr1_4:1196358-1196377 None:intergenic 30.0%
CATGAATTATTACTACGAGA+AGG - chr1_4:1196723-1196742 Msa0142280:CDS 30.0%
!! GCTTCATGTATTATTTCTGA+AGG + chr1_4:1196774-1196793 None:intergenic 30.0%
! TAATGAAAAGCGTAACATAC+AGG - chr1_4:1196881-1196900 Msa0142280:CDS 30.0%
GTAGAAATGATGCTTATTGA+GGG - chr1_4:1196909-1196928 Msa0142280:CDS 30.0%
TCTTGAATATCATGCTTAGT+TGG + chr1_4:1195894-1195913 None:intergenic 30.0%
CGAAATTGTAATCAAACACT+TGG + chr1_4:1195989-1196008 None:intergenic 30.0%
TTTGAAGAAGGAAAGTAGAT+TGG - chr1_4:1196052-1196071 Msa0142280:intron 30.0%
ATTGGATTCAATAAGAGAAG+AGG - chr1_4:1196070-1196089 Msa0142280:intron 30.0%
!! TTGGAATTAGTTTGACTTGA+GGG + chr1_4:1196277-1196296 None:intergenic 30.0%
! TTGGTGATTTATGCTTATGT+TGG + chr1_4:1196358-1196377 None:intergenic 30.0%
CATGAATTATTACTACGAGA+AGG - chr1_4:1196723-1196742 Msa0142280:CDS 30.0%
!! GCTTCATGTATTATTTCTGA+AGG + chr1_4:1196774-1196793 None:intergenic 30.0%
! TAATGAAAAGCGTAACATAC+AGG - chr1_4:1196881-1196900 Msa0142280:CDS 30.0%
GTAGAAATGATGCTTATTGA+GGG - chr1_4:1196909-1196928 Msa0142280:CDS 30.0%
AATAAAACCACAACCACAAC+TGG + chr1_4:1195952-1195971 None:intergenic 35.0%
!!! AGTTGTGGTTGTGGTTTTAT+TGG - chr1_4:1195951-1195970 Msa0142280:CDS 35.0%
!!! GTTGTGGTTGTGGTTTTATT+GGG - chr1_4:1195952-1195971 Msa0142280:CDS 35.0%
ATTCAATAAGAGAAGAGGAG+TGG - chr1_4:1196075-1196094 Msa0142280:intron 35.0%
AGAGAAACAGTAGTTGTAGA+AGG + chr1_4:1196242-1196261 None:intergenic 35.0%
!! GTTGGAATTAGTTTGACTTG+AGG + chr1_4:1196278-1196297 None:intergenic 35.0%
TAAGACAAGGAGTAGTTACA+TGG + chr1_4:1196302-1196321 None:intergenic 35.0%
GTGATTTATGCTTATGTTGG+AGG + chr1_4:1196355-1196374 None:intergenic 35.0%
AAAGCGTAACATACAGGTTA+AGG - chr1_4:1196887-1196906 Msa0142280:CDS 35.0%
GGTAGAAATGATGCTTATTG+AGG - chr1_4:1196908-1196927 Msa0142280:CDS 35.0%
AATAAAACCACAACCACAAC+TGG + chr1_4:1195952-1195971 None:intergenic 35.0%
!!! AGTTGTGGTTGTGGTTTTAT+TGG - chr1_4:1195951-1195970 Msa0142280:CDS 35.0%
!!! GTTGTGGTTGTGGTTTTATT+GGG - chr1_4:1195952-1195971 Msa0142280:CDS 35.0%
ATTCAATAAGAGAAGAGGAG+TGG - chr1_4:1196075-1196094 Msa0142280:intron 35.0%
AGAGAAACAGTAGTTGTAGA+AGG + chr1_4:1196242-1196261 None:intergenic 35.0%
!! GTTGGAATTAGTTTGACTTG+AGG + chr1_4:1196278-1196297 None:intergenic 35.0%
TAAGACAAGGAGTAGTTACA+TGG + chr1_4:1196302-1196321 None:intergenic 35.0%
GTGATTTATGCTTATGTTGG+AGG + chr1_4:1196355-1196374 None:intergenic 35.0%
AAAGCGTAACATACAGGTTA+AGG - chr1_4:1196887-1196906 Msa0142280:CDS 35.0%
GGTAGAAATGATGCTTATTG+AGG - chr1_4:1196908-1196927 Msa0142280:CDS 35.0%
GATGATGAAATCTCCAGTTG+TGG - chr1_4:1195936-1195955 Msa0142280:CDS 40.0%
! TGATGTTGTTTATGTGGCAG+TGG - chr1_4:1196154-1196173 Msa0142280:intron 40.0%
! GATGTTGTTTATGTGGCAGT+GGG - chr1_4:1196155-1196174 Msa0142280:intron 40.0%
! AAATGGTGGAAGTTCAAGCA+TGG - chr1_4:1196181-1196200 Msa0142280:intron 40.0%
!! AGTAGTTGTAGAAGGTGTCA+CGG + chr1_4:1196234-1196253 None:intergenic 40.0%
AAGGAGTAGTTACATGGAGT+TGG + chr1_4:1196296-1196315 None:intergenic 40.0%
AGAAGGTTTCATGAAGCAAG+AGG - chr1_4:1196740-1196759 Msa0142280:CDS 40.0%
AAGCCATTGTGGAACTTGTA+GGG - chr1_4:1196946-1196965 Msa0142280:CDS 40.0%
TTTCCCTACAAGTTCCACAA+TGG + chr1_4:1196952-1196971 None:intergenic 40.0%
GATGATGAAATCTCCAGTTG+TGG - chr1_4:1195936-1195955 Msa0142280:CDS 40.0%
! TGATGTTGTTTATGTGGCAG+TGG - chr1_4:1196154-1196173 Msa0142280:intron 40.0%
! GATGTTGTTTATGTGGCAGT+GGG - chr1_4:1196155-1196174 Msa0142280:intron 40.0%
! AAATGGTGGAAGTTCAAGCA+TGG - chr1_4:1196181-1196200 Msa0142280:intron 40.0%
!! AGTAGTTGTAGAAGGTGTCA+CGG + chr1_4:1196234-1196253 None:intergenic 40.0%
AAGGAGTAGTTACATGGAGT+TGG + chr1_4:1196296-1196315 None:intergenic 40.0%
AGAAGGTTTCATGAAGCAAG+AGG - chr1_4:1196740-1196759 Msa0142280:CDS 40.0%
AAGCCATTGTGGAACTTGTA+GGG - chr1_4:1196946-1196965 Msa0142280:CDS 40.0%
TTTCCCTACAAGTTCCACAA+TGG + chr1_4:1196952-1196971 None:intergenic 40.0%
GAAATCTCCAGTTGTGGTTG+TGG - chr1_4:1195942-1195961 Msa0142280:CDS 45.0%
AATTTCGGAGACGACGATGA+TGG - chr1_4:1196002-1196021 Msa0142280:CDS 45.0%
TATGTGGCAGTGGGAACAAA+TGG - chr1_4:1196164-1196183 Msa0142280:intron 45.0%
!! CAAGCATGGAAGCACTTTCA+TGG - chr1_4:1196195-1196214 Msa0142280:intron 45.0%
TGACTTGAGGGAAAACGTGT+AGG + chr1_4:1196265-1196284 None:intergenic 45.0%
GAAGCCATTGTGGAACTTGT+AGG - chr1_4:1196945-1196964 Msa0142280:CDS 45.0%
GAAATCTCCAGTTGTGGTTG+TGG - chr1_4:1195942-1195961 Msa0142280:CDS 45.0%
AATTTCGGAGACGACGATGA+TGG - chr1_4:1196002-1196021 Msa0142280:CDS 45.0%
TATGTGGCAGTGGGAACAAA+TGG - chr1_4:1196164-1196183 Msa0142280:intron 45.0%
!! CAAGCATGGAAGCACTTTCA+TGG - chr1_4:1196195-1196214 Msa0142280:intron 45.0%
TGACTTGAGGGAAAACGTGT+AGG + chr1_4:1196265-1196284 None:intergenic 45.0%
GAAGCCATTGTGGAACTTGT+AGG - chr1_4:1196945-1196964 Msa0142280:CDS 45.0%
! AGGTGTCACGGCATGTTTCA+AGG + chr1_4:1196222-1196241 None:intergenic 50.0%
CAACGTTGCTGAAGCCATTG+TGG - chr1_4:1196935-1196954 Msa0142280:CDS 50.0%
! AGGTGTCACGGCATGTTTCA+AGG + chr1_4:1196222-1196241 None:intergenic 50.0%
CAACGTTGCTGAAGCCATTG+TGG - chr1_4:1196935-1196954 Msa0142280:CDS 50.0%
GTGGCAGTGGGAACAAATGG+TGG - chr1_4:1196167-1196186 Msa0142280:intron 55.0%
GTGGCAGTGGGAACAAATGG+TGG - chr1_4:1196167-1196186 Msa0142280:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 1195885 1197016 1195885 ID=Msa0142280;Name=Msa0142280
chr1_4 mRNA 1195885 1197016 1195885 ID=Msa0142280-mRNA-1;Parent=Msa0142280;Name=Msa0142280-mRNA-1;_AED=0.04;_eAED=0.06;_QI=0|0|0|1|0|0|2|0|193
chr1_4 exon 1196586 1197016 1196586 ID=Msa0142280-mRNA-1:exon:427;Parent=Msa0142280-mRNA-1
chr1_4 exon 1195885 1196032 1195885 ID=Msa0142280-mRNA-1:exon:426;Parent=Msa0142280-mRNA-1
chr1_4 CDS 1196586 1197016 1196586 ID=Msa0142280-mRNA-1:cds;Parent=Msa0142280-mRNA-1
chr1_4 CDS 1195885 1196032 1195885 ID=Msa0142280-mRNA-1:cds;Parent=Msa0142280-mRNA-1
Gene Sequence

>Msa0142280

ATGATGCCAACTAAGCATGATATTCAAGATGTTCAGTGCTTGCACTACTACGATGATGAAATCTCCAGTTGTGGTTGTGGTTTTATTGGGTTCATGAAAAGCCAAGTGTTTGATTACAATTTCGGAGACGACGATGATGGTGATTCAGAAGAGCTGTTTGAGATAAATTTGAAGAAGGAAAGTAGATTGGATTCAATAAGAGAAGAGGAGTGGTGTGAGAGCAGTACTGTGTTCTCTCTTGATATACACAACAATGATAATAATATAGATGATGTTGTTTATGTGGCAGTGGGAACAAATGGTGGAAGTTCAAGCATGGAAGCACTTTCATGGACCTTGAAACATGCCGTGACACCTTCTACAACTACTGTTTCTCTCCTACACGTTTTCCCTCAAGTCAAACTAATTCCAACTCCATGTAACTACTCCTTTACTAGTAGTAATAATGAAAAGCGTAACATACAGGTTAAGGTAGAAATGATGCTTATTGAGGGTGACAACGTTGCTGAAGCCATTGTGGAACTTGTAGGGAAACTAAATATAAGGAAATTGGTGATTGGAACTACCCAATCTAATCTA

Protein sequence

>Msa0142280

MMPTKHDIQDVQCLHYYDDEISSCGCGFIGFMKSQVFDYNFGDDDDGDSEELFEINLKKESRLDSIREEEWCESSTVFSLDIHNNDNNIDDVVYVAVGTNGGSSSMEALSWTLKHAVTPSTTTVSLLHVFPQVKLIPTPCNYSFTSSNNEKRNIQVKVEMMLIEGDNVAEAIVELVGKLNIRKLVIGTTQSNL