AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1244590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1244590 MtrunA17_Chr8g0360521 97.842 417 9 0 1 417 1 417 0.0 854
Msa1244590 MtrunA17_Chr5g0437601 50.143 349 169 3 66 410 78 425 4.47e-130 381
Msa1244590 MtrunA17_Chr3g0113671 50.704 355 166 4 60 409 99 449 4.72e-130 382
Msa1244590 MtrunA17_Chr4g0005491 46.883 401 192 7 18 410 21 408 4.48e-128 375
Msa1244590 MtrunA17_Chr4g0006991 48.442 353 174 3 64 409 76 427 2.43e-126 372
Msa1244590 MtrunA17_Chr4g0005481 49.708 342 170 2 69 409 106 446 4.17e-124 367
Msa1244590 MtrunA17_Chr3g0114101 49.419 344 169 3 71 410 89 431 1.30e-123 365
Msa1244590 MtrunA17_Chr7g0242701 49.008 353 172 3 64 409 76 427 1.67e-122 362
Msa1244590 MtrunA17_Chr7g0243301 43.902 410 208 8 12 410 12 410 1.66e-120 356
Msa1244590 MtrunA17_Chr5g0437291 45.143 350 184 4 69 410 163 512 3.45e-114 343
Msa1244590 MtrunA17_Chr1g0171171 42.566 343 173 9 71 409 53 375 3.24e-85 265
Msa1244590 MtrunA17_Chr3g0105301 38.617 347 194 6 71 410 43 377 5.98e-83 259
Msa1244590 MtrunA17_Chr1g0210771 39.766 342 185 8 71 409 59 382 7.02e-83 259
Msa1244590 MtrunA17_Chr3g0097891 37.249 349 186 7 68 409 28 350 7.38e-79 247
Msa1244590 MtrunA17_Chr5g0430371 35.165 364 209 8 62 410 49 400 6.25e-78 246
Msa1244590 MtrunA17_Chr7g0258851 35.876 354 200 8 71 410 248 588 2.97e-74 243
Msa1244590 MtrunA17_Chr2g0314801 37.830 341 184 8 71 406 33 350 3.43e-74 235
Msa1244590 MtrunA17_Chr1g0175671 35.593 354 201 8 71 410 170 510 8.48e-74 239
Msa1244590 MtrunA17_Chr4g0068111 34.426 366 201 8 71 416 114 460 9.75e-74 238
Msa1244590 MtrunA17_Chr4g0041011 35.714 350 189 10 67 409 43 363 1.56e-73 234
Msa1244590 MtrunA17_Chr7g0259711 36.364 363 194 12 69 411 89 434 2.45e-73 236
Msa1244590 MtrunA17_Chr2g0287901 39.452 365 187 14 65 411 91 439 3.96e-73 236
Msa1244590 MtrunA17_Chr5g0413271 35.180 361 205 8 62 411 136 478 4.09e-73 237
Msa1244590 MtrunA17_Chr4g0071391 35.613 351 201 11 71 409 109 446 7.33e-73 235
Msa1244590 MtrunA17_Chr3g0085081 37.464 347 184 8 69 409 29 348 2.35e-72 231
Msa1244590 MtrunA17_Chr7g0269991 34.454 357 190 9 71 409 137 467 9.84e-72 233
Msa1244590 MtrunA17_Chr3g0091031 37.143 350 187 8 64 407 24 346 3.01e-71 228
Msa1244590 MtrunA17_Chr2g0283581 36.176 387 210 10 27 409 5 358 3.04e-71 228
Msa1244590 MtrunA17_Chr2g0325481 35.897 351 195 10 64 410 43 367 1.50e-70 226
Msa1244590 MtrunA17_Chr4g0045941 34.971 346 198 8 68 410 30 351 3.20e-69 223
Msa1244590 MtrunA17_Chr5g0413851 34.483 406 213 10 14 412 3 362 6.16e-68 219
Msa1244590 MtrunA17_Chr1g0213281 32.044 362 226 9 60 410 164 516 5.65e-67 222
Msa1244590 MtrunA17_Chr4g0045951 35.028 354 201 9 61 409 33 362 1.56e-66 216
Msa1244590 MtrunA17_Chr3g0085071 35.244 349 195 8 64 407 39 361 1.80e-66 216
Msa1244590 MtrunA17_Chr2g0314791 32.773 357 196 10 67 412 25 348 4.58e-64 209
Msa1244590 MtrunA17_Chr8g0368461 33.243 370 205 11 69 411 176 530 4.62e-63 212
Msa1244590 MtrunA17_Chr3g0126021 32.520 369 209 9 59 411 117 461 4.50e-61 204
Msa1244590 MtrunA17_Chr8g0384151 32.231 363 208 10 65 414 84 421 2.11e-58 197
Msa1244590 MtrunA17_Chr1g0209921 35.217 230 139 6 71 297 44 266 6.01e-49 167
Msa1244590 MtrunA17_Chr6g0476041 29.058 382 214 13 67 415 163 520 2.64e-46 167
Msa1244590 MtrunA17_Chr1g0178081 29.722 360 179 9 69 417 53 349 4.93e-46 162
Msa1244590 MtrunA17_Chr1g0188191 28.760 379 223 15 67 417 230 589 1.04e-44 163
Msa1244590 MtrunA17_Chr8g0347321 29.722 360 217 13 71 409 95 439 5.08e-41 150
Msa1244590 MtrunA17_Chr7g0226991 28.689 366 232 13 64 411 50 404 2.62e-40 148
Msa1244590 MtrunA17_Chr6g0456691 27.934 426 245 15 14 409 26 419 3.77e-40 148
Msa1244590 MtrunA17_Chr8g0391481 28.723 376 222 11 68 417 24 379 6.72e-40 146
Msa1244590 MtrunA17_Chr6g0456681 27.528 356 226 11 69 405 62 404 1.48e-38 144
Msa1244590 MtrunA17_Chr8g0384421 29.201 363 214 12 69 409 131 472 2.62e-38 144
Msa1244590 MtrunA17_Chr5g0414051 28.267 375 214 14 68 411 72 422 3.47e-37 140
Msa1244590 MtrunA17_Chr5g0414091 27.123 424 241 17 18 405 36 427 3.59e-37 140
Msa1244590 MtrunA17_Chr3g0140371 28.065 367 222 12 68 411 15 362 6.35e-37 138
Msa1244590 MtrunA17_Chr2g0283851 29.363 361 208 16 72 405 56 396 8.94e-37 138
Msa1244590 MtrunA17_Chr4g0049421 28.261 368 220 16 71 413 48 396 1.25e-35 135
Msa1244590 MtrunA17_Chr8g0387921 29.121 364 207 16 71 405 74 415 3.39e-35 134
Msa1244590 MtrunA17_Chr5g0414061 28.418 373 209 12 71 411 94 440 4.98e-34 132
Msa1244590 MtrunA17_Chr4g0070341 27.487 382 196 15 69 409 130 471 3.79e-33 129
Msa1244590 MtrunA17_Chr5g0414041 27.151 372 217 11 71 411 87 435 6.41e-33 129
Msa1244590 MtrunA17_Chr6g0455901 25.737 373 220 15 69 409 69 416 5.11e-28 114
Msa1244590 MtrunA17_Chr6g0451321 23.978 367 238 13 65 409 110 457 2.52e-27 113
Msa1244590 MtrunA17_Chr7g0221271 33.333 165 106 3 1 163 8 170 8.22e-26 103
Msa1244590 MtrunA17_Chr7g0221291 32.727 165 107 3 1 163 8 170 1.47e-24 99.4
Msa1244590 MtrunA17_Chr2g0287911 56.140 57 17 2 71 127 61 109 2.61e-17 77.4
Msa1244590 MtrunA17_Chr3g0085101 37.736 106 58 2 265 367 6 106 4.64e-15 71.2
Msa1244590 MtrunA17_Chr2g0287931 31.746 126 77 3 132 249 2 126 5.04e-15 71.2
Msa1244590 MtrunA17_Chr1g0209931 34.286 105 52 2 308 412 8 95 3.73e-12 62.4
Msa1244590 MtrunA17_Chr5g0429191 59.184 49 14 2 355 402 2 45 8.05e-12 59.7
Msa1244590 MtrunA17_Chr7g0221281 22.222 243 149 7 185 405 8 232 4.32e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1244590 AT1G73140.1 68.990 416 124 2 2 416 1 412 0.0 614
Msa1244590 AT5G01360.2 48.760 363 179 2 54 409 16 378 4.23e-132 385
Msa1244590 AT5G01360.1 48.760 363 179 2 54 409 72 434 1.67e-131 385
Msa1244590 AT2G40150.1 49.861 361 166 3 60 409 64 420 2.87e-131 384
Msa1244590 AT3G55990.1 50.144 347 168 2 69 410 138 484 2.51e-130 384
Msa1244590 AT2G40320.1 48.711 349 174 2 66 410 76 423 1.08e-123 365
Msa1244590 AT3G11030.1 48.286 350 175 4 66 410 101 449 2.98e-120 357
Msa1244590 AT5G01620.2 46.686 347 176 4 68 409 107 449 3.83e-120 357
Msa1244590 AT5G01620.1 46.686 347 176 4 68 409 107 449 3.83e-120 357
Msa1244590 AT5G01620.3 46.686 347 176 4 68 409 115 457 4.42e-120 357
Msa1244590 AT2G38320.1 44.104 424 203 8 1 413 8 408 2.01e-118 351
Msa1244590 AT2G40160.1 41.509 424 231 5 4 410 1 424 2.53e-117 349
Msa1244590 AT2G40160.2 41.509 424 231 5 4 410 1 424 2.73e-117 349
Msa1244590 AT2G38320.2 46.483 327 164 5 96 413 1 325 3.44e-100 301
Msa1244590 AT3G54260.1 39.420 345 188 7 71 412 51 377 2.39e-82 258
Msa1244590 AT2G30900.1 38.329 347 184 10 71 410 43 366 7.29e-82 256
Msa1244590 AT3G62390.1 37.778 360 192 11 64 410 132 472 9.36e-80 254
Msa1244590 AT2G40160.3 40.514 311 169 4 4 298 1 311 1.55e-79 248
Msa1244590 AT5G20590.1 36.158 354 200 9 70 410 140 480 1.67e-78 251
Msa1244590 AT5G58600.1 37.176 347 200 6 71 410 66 401 3.42e-78 248
Msa1244590 AT3G12060.1 37.921 356 194 9 69 410 194 536 2.19e-76 248
Msa1244590 AT1G78710.1 35.465 344 194 9 71 410 38 357 3.43e-76 241
Msa1244590 AT2G31110.2 38.304 342 184 8 71 409 45 362 3.24e-74 236
Msa1244590 AT1G29050.1 35.262 363 205 8 50 409 43 378 3.76e-74 236
Msa1244590 AT5G06700.1 37.394 353 198 10 69 409 253 594 2.41e-73 241
Msa1244590 AT2G34070.1 34.278 353 204 7 61 409 55 383 5.74e-73 234
Msa1244590 AT5G49340.1 36.915 363 186 10 71 410 100 442 7.42e-73 236
Msa1244590 AT3G06080.2 36.695 357 200 8 70 411 102 447 2.78e-72 234
Msa1244590 AT1G60790.1 34.197 386 215 12 52 411 162 534 1.72e-69 229
Msa1244590 AT2G42570.1 37.209 344 187 8 71 410 47 365 2.52e-69 224
Msa1244590 AT2G30010.1 33.600 375 209 10 56 410 43 397 4.90e-69 224
Msa1244590 AT2G30010.2 33.600 375 209 10 56 410 86 440 8.30e-69 224
Msa1244590 AT3G14850.2 34.582 347 201 8 69 411 32 356 1.75e-68 221
Msa1244590 AT5G19160.1 34.239 368 208 10 70 417 98 451 2.44e-68 224
Msa1244590 AT5G06230.2 34.973 366 208 8 67 414 17 370 2.05e-58 195
Msa1244590 AT5G06230.1 34.973 366 208 8 67 414 58 411 6.73e-58 195
Msa1244590 AT1G48880.1 32.113 355 199 13 71 411 115 441 1.49e-57 195
Msa1244590 AT1G78710.2 30.294 340 164 8 71 410 31 297 1.78e-56 188
Msa1244590 AT3G11570.1 34.384 349 186 12 88 414 98 425 7.45e-53 182
Msa1244590 AT2G30900.2 38.938 226 123 7 71 290 43 259 1.98e-52 177
Msa1244590 AT3G62390.2 37.247 247 141 7 64 300 132 374 6.48e-52 179
Msa1244590 AT5G20590.2 35.417 240 142 5 70 298 140 377 1.11e-50 175
Msa1244590 AT2G31110.3 38.222 225 128 4 71 292 45 261 2.15e-50 171
Msa1244590 AT5G19160.2 35.146 239 144 4 70 298 98 335 6.86e-49 170
Msa1244590 AT5G58600.2 36.364 231 136 4 71 297 66 289 1.40e-48 167
Msa1244590 AT3G06080.1 35.983 239 142 4 70 298 102 339 2.82e-48 168
Msa1244590 AT2G34070.2 32.353 272 157 6 142 409 1 249 6.68e-46 159
Msa1244590 AT5G51640.1 28.460 383 224 13 64 415 131 494 1.20e-43 159
Msa1244590 AT4G25360.1 28.429 401 222 15 58 417 156 532 1.24e-43 160
Msa1244590 AT4G25360.2 28.429 401 222 15 58 417 156 532 1.24e-43 160
Msa1244590 AT5G64020.1 30.790 367 211 13 71 409 57 408 1.80e-43 157
Msa1244590 AT5G64470.3 28.926 363 221 15 71 414 55 399 1.13e-41 152
Msa1244590 AT1G70230.1 27.920 351 223 11 71 409 79 411 5.41e-41 150
Msa1244590 AT3G14850.1 31.752 274 162 7 142 411 1 253 2.35e-40 144
Msa1244590 AT5G20680.6 29.058 382 190 14 69 409 116 457 1.13e-39 147
Msa1244590 AT5G20680.2 29.058 382 190 14 69 409 192 533 2.65e-39 148
Msa1244590 AT2G37720.1 30.319 376 189 16 71 409 143 482 3.01e-39 147
Msa1244590 AT5G20680.4 29.058 382 190 14 69 409 210 551 3.19e-39 148
Msa1244590 AT5G20680.1 29.058 382 190 14 69 409 210 551 3.19e-39 148
Msa1244590 AT5G20680.3 29.058 382 190 14 69 409 210 551 3.19e-39 148
Msa1244590 AT5G20680.5 29.058 382 190 14 69 409 210 551 3.19e-39 148
Msa1244590 AT3G02440.2 28.212 358 222 11 71 409 135 476 3.90e-39 147
Msa1244590 AT3G02440.1 28.249 354 219 11 71 405 127 464 4.24e-39 146
Msa1244590 AT5G15890.1 27.778 378 230 10 50 405 162 518 8.17e-38 144
Msa1244590 AT5G64470.2 28.455 369 221 17 71 414 55 405 9.64e-38 141
Msa1244590 AT2G14530.1 27.688 372 217 12 67 409 58 406 2.61e-37 140
Msa1244590 AT4G23790.1 27.093 454 241 21 6 405 6 423 3.37e-37 140
Msa1244590 AT5G06230.3 34.274 248 144 5 67 297 58 303 4.61e-37 137
Msa1244590 AT1G01430.1 25.750 400 261 11 34 410 62 448 9.30e-36 137
Msa1244590 AT4G11090.1 26.075 372 227 13 62 405 70 421 2.73e-35 135
Msa1244590 AT4G01080.1 26.776 366 223 13 63 405 83 426 4.96e-34 132
Msa1244590 AT5G15900.1 27.701 361 225 11 69 409 65 409 5.13e-34 132
Msa1244590 AT5G15900.2 27.701 361 225 11 69 409 84 428 7.34e-34 131
Msa1244590 AT3G28150.1 28.767 365 220 15 66 409 65 410 9.61e-34 130
Msa1244590 AT2G31110.1 35.217 230 123 7 183 409 8 214 2.12e-33 125
Msa1244590 AT5G64470.4 26.506 249 162 10 71 303 55 298 7.03e-21 93.2
Msa1244590 AT5G64470.1 26.908 249 161 10 71 303 55 298 7.16e-21 93.2

Find 135 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCAATCAGCAAAAGATTT+TGG 0.125911 8_2:+33247193 None:intergenic
AGAAGTGAATACAATATAAT+TGG 0.171993 8_2:+33249417 None:intergenic
AGGATTTCATTCCATGTATC+TGG 0.201338 8_2:+33247148 None:intergenic
AACAAAAGAATGATGTTTAT+TGG 0.212670 8_2:-33248901 Msa1244590:CDS
CTGATTCTTTCTACACAAAT+TGG 0.216029 8_2:-33249143 Msa1244590:CDS
CAATACAAGGTTCGTCATAT+TGG 0.230048 8_2:-33247390 Msa1244590:CDS
TGGAGGGATTTCTTTCCTTC+AGG 0.275411 8_2:+33248820 None:intergenic
TCTTCATAGGTGGAATTCTT+TGG 0.275642 8_2:+33248800 None:intergenic
CTGTCGTGACGTTATATTCT+TGG 0.282711 8_2:+33247913 None:intergenic
AATACAAGGTTCGTCATATT+GGG 0.282741 8_2:-33247389 Msa1244590:CDS
CCTTATACGATCTAAGCTTT+TGG 0.289384 8_2:-33246125 Msa1244590:intron
AGGTCTCCCATTCTTCTTAC+AGG 0.297921 8_2:+33249164 None:intergenic
TTGGCAAGGAGTGGACATTT+TGG 0.310618 8_2:-33248314 Msa1244590:CDS
GCAATGTATTTGAAGGGAAA+TGG 0.312090 8_2:-33249254 Msa1244590:CDS
TGCATTCACTGGTGTTTACC+TGG 0.316704 8_2:-33247175 Msa1244590:CDS
AACAGATCATAGACTCAAAT+TGG 0.319491 8_2:+33248860 None:intergenic
GCTTGCTCTTCAAGCTATCT+AGG 0.322912 8_2:+33249370 None:intergenic
TAGATCTCTGTTCTTTAGTC+AGG 0.343380 8_2:+33247218 None:intergenic
ATTACCAGTTGTTGTGTTGC+CGG 0.344537 8_2:-33249298 Msa1244590:CDS
ATACTACTGGGCTCCATTCA+TGG 0.353905 8_2:-33248422 Msa1244590:CDS
CGTTGAGTTCACATATGTTC+TGG 0.354464 8_2:-33246693 Msa1244590:three_prime_UTR
GTGGTTTGCTTGACCAAGTA+AGG 0.357337 8_2:+33249186 None:intergenic
ATATAATTGGGAAAATTGAT+TGG 0.370417 8_2:+33249430 None:intergenic
CAATGTATTTGAAGGGAAAT+GGG 0.374415 8_2:-33249253 Msa1244590:CDS
TGCCGAAAATTATATTGTTT+AGG 0.375449 8_2:+33246847 None:intergenic
CAGCTCTACGGCATGTCATC+TGG 0.379386 8_2:-33249333 Msa1244590:CDS
TTATATTCTTGGACTTCATC+TGG 0.380050 8_2:+33247924 None:intergenic
GTGGTATATTTGTGAATGAT+AGG 0.381990 8_2:+33246076 Msa1244580:CDS
ATGCATCGATTGAATACTAC+TGG 0.392477 8_2:-33248435 Msa1244590:CDS
GATGCTCACACATCAGTTTA+CGG 0.394570 8_2:-33247259 Msa1244590:CDS
ATTTGAGTCTATGATCTGTT+TGG 0.397442 8_2:-33248857 Msa1244590:CDS
ATAATTGGGTGATGTTCAAT+AGG 0.397627 8_2:+33247296 None:intergenic
CAATATGACGAACCTTGTAT+TGG 0.400765 8_2:+33247391 None:intergenic
AGAAGAGAGTTGCCCTTACT+TGG 0.406575 8_2:-33249199 Msa1244590:CDS
AGTATGAACAACTAGCAATA+TGG 0.407962 8_2:+33246609 Msa1244580:CDS
TTTAGGTTTCCATGAAGAAC+AGG 0.415460 8_2:+33246864 None:intergenic
TCTACTCTAAATATCTTCAT+AGG 0.419733 8_2:+33248787 None:intergenic
ACGCTCTATTAAATTAAAGT+TGG 0.428012 8_2:+33246014 Msa1244580:CDS
AGGCTAAAATCATAAACTAT+AGG 0.429225 8_2:+33246884 None:intergenic
TCCTCCGGCAACACAACAAC+TGG 0.434980 8_2:+33249294 None:intergenic
CAAATCATACGGTACATAAA+CGG 0.437106 8_2:-33248375 Msa1244590:CDS
CAATTTGTGTAGAAAGAATC+AGG 0.437639 8_2:+33249144 None:intergenic
TCTTAAACAGATATGCATAT+AGG 0.438576 8_2:+33247128 None:intergenic
GAAGTGAATACAATATAATT+GGG 0.442470 8_2:+33249418 None:intergenic
GAACAACTAGCAATATGGTT+TGG 0.449984 8_2:+33246614 Msa1244580:CDS
GAAATTGATTCAACCATGAA+TGG 0.451055 8_2:+33248409 None:intergenic
TTGGGAAAATTGATTGGATC+CGG 0.452959 8_2:+33249436 None:intergenic
AATATGACGAACCTTGTATT+GGG 0.455104 8_2:+33247392 None:intergenic
ACAAGCATCATTCAGATTGA+TGG 0.455770 8_2:-33247058 Msa1244590:three_prime_UTR
CCCTCCTCAATGCGATCCTC+CGG 0.457796 8_2:+33249279 None:intergenic
CATGGGACAGAAAAGAATCC+AGG 0.460825 8_2:+33246553 Msa1244580:intron
TATTTGAAGGGAAATGGGTA+TGG 0.465084 8_2:-33249248 Msa1244590:CDS
TTCTAATCTTTGCAGTTACA+AGG 0.466052 8_2:-33249398 Msa1244590:CDS
GAGGGTTGCAATGTATTTGA+AGG 0.469336 8_2:-33249261 Msa1244590:CDS
GCATTCACTGGTGTTTACCT+GGG 0.470418 8_2:-33247174 Msa1244590:CDS
CTCACGTAGCATTGATGAAA+GGG 0.471007 8_2:+33248260 None:intergenic
CTCTATTAAATTAAAGTTGG+AGG 0.475359 8_2:+33246017 Msa1244580:CDS
CAAACCACCTGTAAGAAGAA+TGG 0.477342 8_2:-33249171 Msa1244590:CDS
AGGAGCTGGGAATGGAAGCC+AGG 0.483811 8_2:-33247445 Msa1244590:CDS
AAACCACCTGTAAGAAGAAT+GGG 0.484872 8_2:-33249170 Msa1244590:CDS
ATCAGTTTACGGCGAGCGAA+AGG 0.487243 8_2:-33247248 Msa1244590:CDS
GGAGATGCTGATATCGAGCC+AGG 0.490893 8_2:+33246635 Msa1244580:CDS
ATCCAAAAGGCAAAAGGTGT+TGG 0.491457 8_2:+33246983 None:intergenic
CATAGGTGGAATTCTTTGGA+GGG 0.491727 8_2:+33248804 None:intergenic
CAACTCTCTTCTTCATAAAG+AGG 0.502052 8_2:+33249210 None:intergenic
ATTCTTTCTACACAAATTGG+AGG 0.504866 8_2:-33249140 Msa1244590:CDS
GTATGTCTCCAACACATTTG+TGG 0.505067 8_2:-33247809 Msa1244590:intron
CATTGATATGAGATGGTTGA+AGG 0.506584 8_2:+33249485 None:intergenic
TGTTCGAGAGTTATGTTTGG+TGG 0.513573 8_2:-33248291 Msa1244590:CDS
GCCGGAGGATCGCATTGAGG+AGG 0.515226 8_2:-33249280 Msa1244590:CDS
TCTTCTCCAGGAGCTGGGAA+TGG 0.516727 8_2:-33247453 Msa1244590:intron
TATACAATCCAAAAGGCAAA+AGG 0.521640 8_2:+33246977 None:intergenic
GATACAAAGCGTGAAGATAG+TGG 0.522290 8_2:+33246057 Msa1244580:CDS
TCATAGGTGGAATTCTTTGG+AGG 0.523486 8_2:+33248803 None:intergenic
GGATTTCATTCCATGTATCT+GGG 0.524709 8_2:+33247149 None:intergenic
GAAAAGAAAGCAAAATAGAA+TGG 0.525233 8_2:+33247005 None:intergenic
TCTTCGAAGAGAATGCATCC+TGG 0.528389 8_2:-33246571 Msa1244590:three_prime_UTR
TACCTGGGGTCCCAGATACA+TGG 0.530934 8_2:-33247159 Msa1244590:CDS
AATTGGAGGTGGCAGCCTCA+TGG 0.531507 8_2:-33249126 Msa1244590:CDS
GTTGCAAATGTACATGTCAA+AGG 0.533678 8_2:+33246814 None:intergenic
TCAGTTTACGGCGAGCGAAA+GGG 0.533883 8_2:-33247247 Msa1244590:CDS
TTCCATGTATCTGGGACCCC+AGG 0.534611 8_2:+33247157 None:intergenic
GGTTCGTCATATTGGGGCAC+AGG 0.534995 8_2:-33247382 Msa1244590:CDS
TGGTGTTCGAGAGTTATGTT+TGG 0.536569 8_2:-33248294 Msa1244590:CDS
TCTCCCATTCTTCTTACAGG+TGG 0.537869 8_2:+33249167 None:intergenic
GTTCTGTTATACAATCCAAA+AGG 0.542969 8_2:+33246970 None:intergenic
GAATTAAATCGTCTGAGAGT+TGG 0.543056 8_2:-33246671 Msa1244590:three_prime_UTR
CATTCACTGGTGTTTACCTG+GGG 0.545869 8_2:-33247173 Msa1244590:CDS
CTTTCTACACAAATTGGAGG+TGG 0.550918 8_2:-33249137 Msa1244590:CDS
GTACAATCTGTAATTCCTGA+AGG 0.559209 8_2:-33248835 Msa1244590:CDS
TGGTATATTTGTGAATGATA+GGG 0.561774 8_2:+33246077 Msa1244580:CDS
ATGCATTCTCTTCGAAGAAG+AGG 0.567019 8_2:+33246576 Msa1244580:CDS
AGCAAGCTTCTGAAACTGAG+TGG 0.568778 8_2:+33246729 Msa1244580:CDS
TAATTGGGTGATGTTCAATA+GGG 0.568867 8_2:+33247297 None:intergenic
TTAAATCGTCTGAGAGTTGG+TGG 0.569032 8_2:-33246668 Msa1244590:three_prime_UTR
CCGGAGGATCGCATTGAGGA+GGG 0.569780 8_2:-33249279 Msa1244590:CDS
ACTCACGTAGCATTGATGAA+AGG 0.570089 8_2:+33248259 None:intergenic
GTAACTTACCACAAATGTGT+TGG 0.570891 8_2:+33247801 None:intergenic
AACATGTGCAACAACTTCAA+AGG 0.571932 8_2:+33248928 None:intergenic
TGCTGTAAATAATAATCAAG+TGG 0.573884 8_2:+33246756 None:intergenic
CGTCACGACAGCATATAAGT+TGG 0.576399 8_2:-33247901 Msa1244590:CDS
GCAAATGTACATGTCAAAGG+TGG 0.579152 8_2:+33246817 None:intergenic
TAGCTAAGCATGGCAAACAT+TGG 0.579693 8_2:-33248333 Msa1244590:CDS
ATTTGAAGGGAAATGGGTAT+GGG 0.582984 8_2:-33249247 Msa1244590:CDS
GCAAGCTTCTGAAACTGAGT+GGG 0.583735 8_2:+33246730 Msa1244580:CDS
TGAAGTTGTTGCACATGTTG+AGG 0.585215 8_2:-33248924 Msa1244590:CDS
AGGGTTGCAATGTATTTGAA+GGG 0.588210 8_2:-33249260 Msa1244590:CDS
TCAAGTCTTCGATTGATCGC+CGG 0.588728 8_2:-33249455 Msa1244590:CDS
TTCGAAGAAGAGGTTAGCAA+AGG 0.592548 8_2:+33246586 Msa1244580:CDS
TGATCATGCAACAAATCATA+CGG 0.594915 8_2:-33248386 Msa1244590:CDS
CTCATGGATGCAACCTCCCA+AGG 0.597976 8_2:-33249110 Msa1244590:intron
TGGCAAACATTGGCAAGGAG+TGG 0.599503 8_2:-33248323 Msa1244590:CDS
CTCTTCTTCATAAAGAGGAT+AGG 0.600163 8_2:+33249215 None:intergenic
TATGAAGATATTTAGAGTAG+AGG 0.600575 8_2:-33248785 Msa1244590:intron
ATTGGAGACTCATTACAAAG+AGG 0.601036 8_2:-33248883 Msa1244590:CDS
ACCAGTTGTTGTGTTGCCGG+AGG 0.602457 8_2:-33249295 Msa1244590:CDS
ACGGCATGTCATCTGGTGGA+AGG 0.604816 8_2:-33249326 Msa1244590:CDS
TGGTTTGCTTGACCAAGTAA+GGG 0.606119 8_2:+33249187 None:intergenic
CTTGGGAGGTTGCATCCATG+AGG 0.606796 8_2:+33249111 None:intergenic
CAAATGTACATGTCAAAGGT+GGG 0.612052 8_2:+33246818 None:intergenic
CTTGACTCAATAGCTAAGCA+TGG 0.612907 8_2:-33248343 Msa1244590:CDS
CTCTACGGCATGTCATCTGG+TGG 0.613992 8_2:-33249330 Msa1244590:CDS
AGCATCATTCAGATTGATGG+TGG 0.614413 8_2:-33247055 Msa1244590:three_prime_UTR
ACTCTAAATATCTTCATAGG+TGG 0.615508 8_2:+33248790 None:intergenic
AAAGTGTTGGTTACCTTGGG+AGG 0.620369 8_2:+33249097 None:intergenic
TAAATAAGACACAAATGCTG+TGG 0.621001 8_2:+33246783 None:intergenic
GTTGCCGGAGGATCGCATTG+AGG 0.623555 8_2:-33249283 Msa1244590:CDS
TCATACGGTACATAAACGGA+TGG 0.623999 8_2:-33248371 Msa1244590:CDS
AATGAATCATACCCAATACA+AGG 0.626400 8_2:-33247403 Msa1244590:CDS
GTTGGTGGTAAATCTTGACC+TGG 0.628676 8_2:-33246653 Msa1244590:three_prime_UTR
GAGATACAAACTGTAAACAA+AGG 0.634145 8_2:-33246924 Msa1244590:three_prime_UTR
TGCATCGATTGAATACTACT+GGG 0.637341 8_2:-33248434 Msa1244590:CDS
AAGCATGGCAAACATTGGCA+AGG 0.639024 8_2:-33248328 Msa1244590:CDS
GAGACAGCATTGATATGAGA+TGG 0.642148 8_2:+33249478 None:intergenic
ATACAAGGTTCGTCATATTG+GGG 0.647384 8_2:-33247388 Msa1244590:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCAATTTTTTATTTTATTC+TGG - chr8_2:33248007-33248026 Msa1244590:intron 10.0%
!!! ATTTTTTTTAAGTTCACAAT+CGG + chr8_2:33246490-33246509 Msa1244580:intron 15.0%
!!! AAATTATTATAGCTTTGTTT+TGG - chr8_2:33247056-33247075 Msa1244590:three_prime_UTR 15.0%
!! TAATAACTCTAGTTATTATT+AGG + chr8_2:33247912-33247931 None:intergenic 15.0%
!!! AACCTAAACAATATAATTTT+CGG - chr8_2:33248675-33248694 Msa1244590:intron 15.0%
!! ATATAATTGGGAAAATTGAT+TGG + chr8_2:33246097-33246116 Msa1244580:CDS 20.0%
!! GAAGTGAATACAATATAATT+GGG + chr8_2:33246109-33246128 Msa1244580:CDS 20.0%
!! AGAAGTGAATACAATATAAT+TGG + chr8_2:33246110-33246129 Msa1244580:CDS 20.0%
!! AACAAAAGAATGATGTTTAT+TGG - chr8_2:33246623-33246642 Msa1244590:three_prime_UTR 20.0%
!!! ATCTGTATTTTTCAAAACAA+TGG - chr8_2:33246782-33246801 Msa1244590:three_prime_UTR 20.0%
!! TTAAGTTATTTATGAAAGCA+TGG + chr8_2:33246808-33246827 None:intergenic 20.0%
!! GATATAAATATTTGTGAGAT+TGG + chr8_2:33246937-33246956 None:intergenic 20.0%
!!! GCTAGAATTTACTTTTTAAA+GGG + chr8_2:33247393-33247412 None:intergenic 20.0%
!! AATCAGACAAATTATTGAAT+TGG - chr8_2:33247545-33247564 Msa1244590:intron 20.0%
!!! AAAAATACATTTTGAGTAAG+TGG + chr8_2:33247690-33247709 None:intergenic 20.0%
!!! ATTATCAAGTTTTGCTAATA+AGG - chr8_2:33247782-33247801 Msa1244590:intron 20.0%
!!! CTTGAGTTTTATTCAAAAAA+TGG + chr8_2:33249080-33249099 None:intergenic 20.0%
!!! CATTTTTTGAATAAAACTCA+AGG - chr8_2:33249078-33249097 Msa1244590:intron 20.0%
!!! TTAAAGTGTTTTTCTTAGAA+TGG + chr8_2:33249194-33249213 None:intergenic 20.0%
!! GAATAAGTTGTTCTATATAT+TGG + chr8_2:33249221-33249240 None:intergenic 20.0%
! TCTACTCTAAATATCTTCAT+AGG + chr8_2:33246740-33246759 Msa1244580:CDS 25.0%
!! TATGAAGATATTTAGAGTAG+AGG - chr8_2:33246739-33246758 Msa1244590:three_prime_UTR 25.0%
!!! CTTTTTAAAGGGAATAAGTA+AGG + chr8_2:33247382-33247401 None:intergenic 25.0%
!!! GGCTAGAATTTACTTTTTAA+AGG + chr8_2:33247394-33247413 None:intergenic 25.0%
!!! AGAAATTTATTTTCTTCTCC+AGG - chr8_2:33248059-33248078 Msa1244590:intron 25.0%
!! TTTTCTGTACTCTGATAATT+GGG + chr8_2:33248245-33248264 None:intergenic 25.0%
! TCTTAAACAGATATGCATAT+AGG + chr8_2:33248399-33248418 None:intergenic 25.0%
! GAAAAGAAAGCAAAATAGAA+TGG + chr8_2:33248522-33248541 None:intergenic 25.0%
! AGGCTAAAATCATAAACTAT+AGG + chr8_2:33248643-33248662 None:intergenic 25.0%
! TGCCGAAAATTATATTGTTT+AGG + chr8_2:33248680-33248699 None:intergenic 25.0%
! TGCTGTAAATAATAATCAAG+TGG + chr8_2:33248771-33248790 None:intergenic 25.0%
!! CATTTTGTAGAAAAGACTAA+TGG - chr8_2:33249248-33249267 Msa1244590:CDS 25.0%
! GACCAAAAAAAAAAGACTAA+TGG - chr8_2:33249372-33249391 Msa1244590:CDS 25.0%
!!! CTCCATTAGTCTTTTTTTTT+TGG + chr8_2:33249377-33249396 None:intergenic 25.0%
! TGGTATATTTGTGAATGATA+GGG + chr8_2:33249450-33249469 None:intergenic 25.0%
! CTCTATTAAATTAAAGTTGG+AGG + chr8_2:33249510-33249529 None:intergenic 25.0%
! ACGCTCTATTAAATTAAAGT+TGG + chr8_2:33249513-33249532 None:intergenic 25.0%
TTCTAATCTTTGCAGTTACA+AGG - chr8_2:33246126-33246145 Msa1244590:intron 30.0%
CAATGTATTTGAAGGGAAAT+GGG - chr8_2:33246271-33246290 Msa1244590:intron 30.0%
CAATTTGTGTAGAAAGAATC+AGG + chr8_2:33246383-33246402 Msa1244580:intron 30.0%
CTGATTCTTTCTACACAAAT+TGG - chr8_2:33246381-33246400 Msa1244590:intron 30.0%
ATTCTTTCTACACAAATTGG+AGG - chr8_2:33246384-33246403 Msa1244590:intron 30.0%
! GTTCAAATGTGAAAAAGTGT+TGG + chr8_2:33246443-33246462 Msa1244580:intron 30.0%
CTGAAGTCAAGAAAAGAATA+AGG + chr8_2:33246569-33246588 Msa1244580:CDS 30.0%
! CTTATTCTTTTCTTGACTTC+AGG - chr8_2:33246567-33246586 Msa1244590:three_prime_UTR 30.0%
AACAGATCATAGACTCAAAT+TGG + chr8_2:33246667-33246686 Msa1244580:CDS 30.0%
ATTTGAGTCTATGATCTGTT+TGG - chr8_2:33246667-33246686 Msa1244590:three_prime_UTR 30.0%
ACTCTAAATATCTTCATAGG+TGG + chr8_2:33246737-33246756 Msa1244580:CDS 30.0%
!!! TATTATAGCTTTGTTTTGGC+AGG - chr8_2:33247060-33247079 Msa1244590:three_prime_UTR 30.0%
! GAAATTGATTCAACCATGAA+TGG + chr8_2:33247118-33247137 None:intergenic 30.0%
TGATCATGCAACAAATCATA+CGG - chr8_2:33247138-33247157 Msa1244590:CDS 30.0%
CAAATCATACGGTACATAAA+CGG - chr8_2:33247149-33247168 Msa1244590:CDS 30.0%
GAACAATGCATTATATGCAA+GGG - chr8_2:33247335-33247354 Msa1244590:CDS 30.0%
! CTTTTTGTCAAGTATTTTCG+TGG + chr8_2:33247415-33247434 None:intergenic 30.0%
TCAAGTACATCTCTATTCAA+GGG + chr8_2:33247441-33247460 None:intergenic 30.0%
TTCAAGTACATCTCTATTCA+AGG + chr8_2:33247442-33247461 None:intergenic 30.0%
TGTACTTGAATGAATGTTCA+TGG - chr8_2:33247452-33247471 Msa1244590:intron 30.0%
!! TGTGCTTTTTACACAACTAT+AGG + chr8_2:33247484-33247503 None:intergenic 30.0%
! GTTGTGTAAAAAGCACAAAA+TGG - chr8_2:33247487-33247506 Msa1244590:intron 30.0%
!! AACATGTTTTGTTCCAGATA+TGG - chr8_2:33247573-33247592 Msa1244590:intron 30.0%
TTATATTCTTGGACTTCATC+TGG + chr8_2:33247603-33247622 None:intergenic 30.0%
!! ATAAGTTGGCCTTAAAAACA+TGG - chr8_2:33247637-33247656 Msa1244590:intron 30.0%
!! TAAGTTGGCCTTAAAAACAT+GGG - chr8_2:33247638-33247657 Msa1244590:intron 30.0%
!! ATACATTTTGAGTAAGTGGT+TGG + chr8_2:33247686-33247705 None:intergenic 30.0%
AAACATTCACCAAATCATGT+GGG + chr8_2:33247868-33247887 None:intergenic 30.0%
AATGAATCATACCCAATACA+AGG - chr8_2:33248121-33248140 Msa1244590:intron 30.0%
AATATGACGAACCTTGTATT+GGG + chr8_2:33248135-33248154 None:intergenic 30.0%
AATACAAGGTTCGTCATATT+GGG - chr8_2:33248135-33248154 Msa1244590:intron 30.0%
TAATTGGGTGATGTTCAATA+GGG + chr8_2:33248230-33248249 None:intergenic 30.0%
ATAATTGGGTGATGTTCAAT+AGG + chr8_2:33248231-33248250 None:intergenic 30.0%
! CTTTTCTGTACTCTGATAAT+TGG + chr8_2:33248246-33248265 None:intergenic 30.0%
! TGCAATCAGCAAAAGATTTT+GGG + chr8_2:33248333-33248352 None:intergenic 30.0%
! ATGCAATCAGCAAAAGATTT+TGG + chr8_2:33248334-33248353 None:intergenic 30.0%
TATACAATCCAAAAGGCAAA+AGG + chr8_2:33248550-33248569 None:intergenic 30.0%
GTTCTGTTATACAATCCAAA+AGG + chr8_2:33248557-33248576 None:intergenic 30.0%
GAGATACAAACTGTAAACAA+AGG - chr8_2:33248600-33248619 Msa1244590:intron 30.0%
TAAATAAGACACAAATGCTG+TGG + chr8_2:33248744-33248763 None:intergenic 30.0%
AGTATGAACAACTAGCAATA+TGG + chr8_2:33248918-33248937 None:intergenic 30.0%
!!! CCATTACTGTCATTTTTCAT+GGG + chr8_2:33248991-33249010 None:intergenic 30.0%
!!! TCCATTACTGTCATTTTTCA+TGG + chr8_2:33248992-33249011 None:intergenic 30.0%
GACATTGCATATTATATGCA+GGG - chr8_2:33249278-33249297 Msa1244590:CDS 30.0%
ACATTGCATATTATATGCAG+GGG - chr8_2:33249279-33249298 Msa1244590:CDS 30.0%
GTGGTATATTTGTGAATGAT+AGG + chr8_2:33249451-33249470 None:intergenic 30.0%
CATTGATATGAGATGGTTGA+AGG + chr8_2:33246042-33246061 Msa1244580:CDS 35.0%
TTGGGAAAATTGATTGGATC+CGG + chr8_2:33246091-33246110 Msa1244580:CDS 35.0%
AGGGTTGCAATGTATTTGAA+GGG - chr8_2:33246264-33246283 Msa1244590:intron 35.0%
GCAATGTATTTGAAGGGAAA+TGG - chr8_2:33246270-33246289 Msa1244590:intron 35.0%
TATTTGAAGGGAAATGGGTA+TGG - chr8_2:33246276-33246295 Msa1244590:intron 35.0%
ATTTGAAGGGAAATGGGTAT+GGG - chr8_2:33246277-33246296 Msa1244590:intron 35.0%
CTCTTCTTCATAAAGAGGAT+AGG + chr8_2:33246312-33246331 Msa1244580:intron 35.0%
CAACTCTCTTCTTCATAAAG+AGG + chr8_2:33246317-33246336 Msa1244580:intron 35.0%
AAACCACCTGTAAGAAGAAT+GGG - chr8_2:33246354-33246373 Msa1244590:intron 35.0%
!! GAAAAAGTGTTGGTTACCTT+GGG + chr8_2:33246433-33246452 Msa1244580:intron 35.0%
!! TGAAAAAGTGTTGGTTACCT+TGG + chr8_2:33246434-33246453 Msa1244580:intron 35.0%
CGGAAATCTAAGAGTAATCA+AGG + chr8_2:33246470-33246489 Msa1244580:intron 35.0%
AACATGTGCAACAACTTCAA+AGG + chr8_2:33246599-33246618 Msa1244580:CDS 35.0%
ATTGGAGACTCATTACAAAG+AGG - chr8_2:33246641-33246660 Msa1244590:three_prime_UTR 35.0%
GTACAATCTGTAATTCCTGA+AGG - chr8_2:33246689-33246708 Msa1244590:three_prime_UTR 35.0%
TCTTCATAGGTGGAATTCTT+TGG + chr8_2:33246727-33246746 Msa1244580:CDS 35.0%
!! ATGCATCGATTGAATACTAC+TGG - chr8_2:33247089-33247108 Msa1244590:exon 35.0%
!! TGCATCGATTGAATACTACT+GGG - chr8_2:33247090-33247109 Msa1244590:exon 35.0%
GTGAGTATATCTATAGACCT+TGG - chr8_2:33247282-33247301 Msa1244590:CDS 35.0%
GGAACAATGCATTATATGCA+AGG - chr8_2:33247334-33247353 Msa1244590:CDS 35.0%
!! CCAATTTGCCCATGTTTTTA+AGG + chr8_2:33247649-33247668 None:intergenic 35.0%
CCTTAAAAACATGGGCAAAT+TGG - chr8_2:33247646-33247665 Msa1244590:intron 35.0%
GTAACTTACCACAAATGTGT+TGG + chr8_2:33247726-33247745 None:intergenic 35.0%
GCAGAGTCACTCTTTAAATT+TGG + chr8_2:33247828-33247847 None:intergenic 35.0%
CAAACATTCACCAAATCATG+TGG + chr8_2:33247869-33247888 None:intergenic 35.0%
CAATATGACGAACCTTGTAT+TGG + chr8_2:33248136-33248155 None:intergenic 35.0%
CAATACAAGGTTCGTCATAT+TGG - chr8_2:33248134-33248153 Msa1244590:intron 35.0%
ATACAAGGTTCGTCATATTG+GGG - chr8_2:33248136-33248155 Msa1244590:intron 35.0%
TAGATCTCTGTTCTTTAGTC+AGG + chr8_2:33248309-33248328 None:intergenic 35.0%
GGATTTCATTCCATGTATCT+GGG + chr8_2:33248378-33248397 None:intergenic 35.0%
AGGATTTCATTCCATGTATC+TGG + chr8_2:33248379-33248398 None:intergenic 35.0%
ACAAGCATCATTCAGATTGA+TGG - chr8_2:33248466-33248485 Msa1244590:intron 35.0%
!! TGATTTTAGCCTGTTCTTCA+TGG - chr8_2:33248651-33248670 Msa1244590:intron 35.0%
! TTTAGGTTTCCATGAAGAAC+AGG + chr8_2:33248663-33248682 None:intergenic 35.0%
CAAATGTACATGTCAAAGGT+GGG + chr8_2:33248709-33248728 None:intergenic 35.0%
GTTGCAAATGTACATGTCAA+AGG + chr8_2:33248713-33248732 None:intergenic 35.0%
GAATTAAATCGTCTGAGAGT+TGG - chr8_2:33248853-33248872 Msa1244590:CDS 35.0%
GAACAACTAGCAATATGGTT+TGG + chr8_2:33248913-33248932 None:intergenic 35.0%
CCCATGAAAAATGACAGTAA+TGG - chr8_2:33248988-33249007 Msa1244590:intron 35.0%
TAGAAAAGACTAATGGAGTC+AGG - chr8_2:33249255-33249274 Msa1244590:CDS 35.0%
AGAAAAGACTAATGGAGTCA+GGG - chr8_2:33249256-33249275 Msa1244590:CDS 35.0%
GGACATTGCATATTATATGC+AGG - chr8_2:33249277-33249296 Msa1244590:CDS 35.0%
CTCATAAAGGTGAATAAGTG+GGG + chr8_2:33249326-33249345 None:intergenic 35.0%
CCTCATAAAGGTGAATAAGT+GGG + chr8_2:33249327-33249346 None:intergenic 35.0%
ACCTCATAAAGGTGAATAAG+TGG + chr8_2:33249328-33249347 None:intergenic 35.0%
GCTAGAAATTCACCTCATAA+AGG + chr8_2:33249339-33249358 None:intergenic 35.0%
CCAAAAGCTTAGATCGTATA+AGG + chr8_2:33249402-33249421 None:intergenic 35.0%
! CCTTATACGATCTAAGCTTT+TGG - chr8_2:33249399-33249418 Msa1244590:CDS 35.0%
!! GAGACAGCATTGATATGAGA+TGG + chr8_2:33246049-33246068 Msa1244580:CDS 40.0%
!! AGCCATGTTTTTCAGCTCTA+CGG - chr8_2:33246179-33246198 Msa1244590:intron 40.0%
ATTACCAGTTGTTGTGTTGC+CGG - chr8_2:33246226-33246245 Msa1244590:intron 40.0%
GAGGGTTGCAATGTATTTGA+AGG - chr8_2:33246263-33246282 Msa1244590:intron 40.0%
!! TGGTTTGCTTGACCAAGTAA+GGG + chr8_2:33246340-33246359 Msa1244580:intron 40.0%
CAAACCACCTGTAAGAAGAA+TGG - chr8_2:33246353-33246372 Msa1244590:intron 40.0%
CTTTCTACACAAATTGGAGG+TGG - chr8_2:33246387-33246406 Msa1244590:intron 40.0%
TGAAGTTGTTGCACATGTTG+AGG - chr8_2:33246600-33246619 Msa1244590:three_prime_UTR 40.0%
CATAGGTGGAATTCTTTGGA+GGG + chr8_2:33246723-33246742 Msa1244580:CDS 40.0%
TCATAGGTGGAATTCTTTGG+AGG + chr8_2:33246724-33246743 Msa1244580:CDS 40.0%
TCATACGGTACATAAACGGA+TGG - chr8_2:33247153-33247172 Msa1244590:CDS 40.0%
CTTGACTCAATAGCTAAGCA+TGG - chr8_2:33247181-33247200 Msa1244590:CDS 40.0%
TAGCTAAGCATGGCAAACAT+TGG - chr8_2:33247191-33247210 Msa1244590:CDS 40.0%
TGGTGTTCGAGAGTTATGTT+TGG - chr8_2:33247230-33247249 Msa1244590:CDS 40.0%
TGTTCGAGAGTTATGTTTGG+TGG - chr8_2:33247233-33247252 Msa1244590:CDS 40.0%
!! CTCACGTAGCATTGATGAAA+GGG + chr8_2:33247267-33247286 None:intergenic 40.0%
!! ACTCACGTAGCATTGATGAA+AGG + chr8_2:33247268-33247287 None:intergenic 40.0%
GTATATCTATAGACCTTGGC+TGG - chr8_2:33247286-33247305 Msa1244590:CDS 40.0%
AATGCATTATATGCAAGGGC+CGG - chr8_2:33247339-33247358 Msa1244590:CDS 40.0%
TTCATCTGGTGATCCATATC+TGG + chr8_2:33247589-33247608 None:intergenic 40.0%
CTGTCGTGACGTTATATTCT+TGG + chr8_2:33247614-33247633 None:intergenic 40.0%
GTATGTCTCCAACACATTTG+TGG - chr8_2:33247715-33247734 Msa1244590:intron 40.0%
AAAGTGAAGCCCACATGATT+TGG - chr8_2:33247856-33247875 Msa1244590:CDS 40.0%
! TTATTTTCTTCTCCAGGAGC+TGG - chr8_2:33248065-33248084 Msa1244590:intron 40.0%
! TATTTTCTTCTCCAGGAGCT+GGG - chr8_2:33248066-33248085 Msa1244590:intron 40.0%
! AAGCAGTTTTCATCACTTCC+TGG + chr8_2:33248100-33248119 None:intergenic 40.0%
GATGCTCACACATCAGTTTA+CGG - chr8_2:33248265-33248284 Msa1244590:CDS 40.0%
!! CTTTTGCTGATTGCATTCAC+TGG - chr8_2:33248338-33248357 Msa1244590:CDS 40.0%
AGCATCATTCAGATTGATGG+TGG - chr8_2:33248469-33248488 Msa1244590:intron 40.0%
! TTCCAACACCTTTTGCCTTT+TGG - chr8_2:33248539-33248558 Msa1244590:intron 40.0%
! ATCCAAAAGGCAAAAGGTGT+TGG + chr8_2:33248544-33248563 None:intergenic 40.0%
GCAAATGTACATGTCAAAGG+TGG + chr8_2:33248710-33248729 None:intergenic 40.0%
CGTTGAGTTCACATATGTTC+TGG - chr8_2:33248831-33248850 Msa1244590:CDS 40.0%
TTAAATCGTCTGAGAGTTGG+TGG - chr8_2:33248856-33248875 Msa1244590:CDS 40.0%
TTCGAAGAAGAGGTTAGCAA+AGG + chr8_2:33248941-33248960 None:intergenic 40.0%
ATGCATTCTCTTCGAAGAAG+AGG + chr8_2:33248951-33248970 None:intergenic 40.0%
TCAAGGAAACGAGTAAGATG+TGG - chr8_2:33249095-33249114 Msa1244590:intron 40.0%
CCCACTTATTCACCTTTATG+AGG - chr8_2:33249324-33249343 Msa1244590:CDS 40.0%
!! TTTTTGGTCTAGTAGCCTAG+TGG + chr8_2:33249361-33249380 None:intergenic 40.0%
GATACAAAGCGTGAAGATAG+TGG + chr8_2:33249470-33249489 None:intergenic 40.0%
TCAAGTCTTCGATTGATCGC+CGG - chr8_2:33246069-33246088 Msa1244590:three_prime_UTR 45.0%
GCTTGCTCTTCAAGCTATCT+AGG + chr8_2:33246157-33246176 Msa1244580:intron 45.0%
TGCCGTAGAGCTGAAAAACA+TGG + chr8_2:33246184-33246203 Msa1244580:intron 45.0%
AGAAGAGAGTTGCCCTTACT+TGG - chr8_2:33246325-33246344 Msa1244590:intron 45.0%
!! GTGGTTTGCTTGACCAAGTA+AGG + chr8_2:33246341-33246360 Msa1244580:intron 45.0%
TCTCCCATTCTTCTTACAGG+TGG + chr8_2:33246360-33246379 Msa1244580:intron 45.0%
AGGTCTCCCATTCTTCTTAC+AGG + chr8_2:33246363-33246382 Msa1244580:intron 45.0%
!! AAAGTGTTGGTTACCTTGGG+AGG + chr8_2:33246430-33246449 Msa1244580:intron 45.0%
TGGAGGGATTTCTTTCCTTC+AGG + chr8_2:33246707-33246726 Msa1244580:CDS 45.0%
ATACTACTGGGCTCCATTCA+TGG - chr8_2:33247102-33247121 Msa1244590:exon 45.0%
AAGCATGGCAAACATTGGCA+AGG - chr8_2:33247196-33247215 Msa1244590:CDS 45.0%
! TTGGCAAGGAGTGGACATTT+TGG - chr8_2:33247210-33247229 Msa1244590:CDS 45.0%
TGTCAACTGAGCTATGCTCA+CGG + chr8_2:33247314-33247333 None:intergenic 45.0%
! GGTGTTTGAAGTTCGAACTC+CGG + chr8_2:33247361-33247380 None:intergenic 45.0%
CGTCACGACAGCATATAAGT+TGG - chr8_2:33247623-33247642 Msa1244590:intron 45.0%
TGCATTCACTGGTGTTTACC+TGG - chr8_2:33248349-33248368 Msa1244590:CDS 45.0%
GCATTCACTGGTGTTTACCT+GGG - chr8_2:33248350-33248369 Msa1244590:CDS 45.0%
CATTCACTGGTGTTTACCTG+GGG - chr8_2:33248351-33248370 Msa1244590:CDS 45.0%
!! GCAAGCTTCTGAAACTGAGT+GGG + chr8_2:33248797-33248816 None:intergenic 45.0%
!! AGCAAGCTTCTGAAACTGAG+TGG + chr8_2:33248798-33248817 None:intergenic 45.0%
!! GTTGGTGGTAAATCTTGACC+TGG - chr8_2:33248871-33248890 Msa1244590:CDS 45.0%
TCTTCGAAGAGAATGCATCC+TGG - chr8_2:33248953-33248972 Msa1244590:intron 45.0%
CATGGGACAGAAAAGAATCC+AGG + chr8_2:33248974-33248993 None:intergenic 45.0%
TGCATATTATATGCAGGGGC+CGG - chr8_2:33249283-33249302 Msa1244590:CDS 45.0%
!! GAGGTGAATTTCTAGCCACT+AGG - chr8_2:33249343-33249362 Msa1244590:CDS 45.0%
TGGCAAACATTGGCAAGGAG+TGG - chr8_2:33247201-33247220 Msa1244590:CDS 50.0%
GTCAACTGAGCTATGCTCAC+GGG + chr8_2:33247313-33247332 None:intergenic 50.0%
GTGAGCATAGCTCAGTTGAC+AGG - chr8_2:33247313-33247332 Msa1244590:CDS 50.0%
ATCAGTTTACGGCGAGCGAA+AGG - chr8_2:33248276-33248295 Msa1244590:CDS 50.0%
TCAGTTTACGGCGAGCGAAA+GGG - chr8_2:33248277-33248296 Msa1244590:CDS 50.0%
GCATATTATATGCAGGGGCC+GGG - chr8_2:33249284-33249303 Msa1244590:CDS 50.0%
AGGTGAATAAGTGGGGAGTC+CGG + chr8_2:33249319-33249338 None:intergenic 50.0%
CAGCTCTACGGCATGTCATC+TGG - chr8_2:33246191-33246210 Msa1244590:intron 55.0%
CTCTACGGCATGTCATCTGG+TGG - chr8_2:33246194-33246213 Msa1244590:intron 55.0%
ACGGCATGTCATCTGGTGGA+AGG - chr8_2:33246198-33246217 Msa1244590:intron 55.0%
ACCAGTTGTTGTGTTGCCGG+AGG - chr8_2:33246229-33246248 Msa1244590:intron 55.0%
TCCTCCGGCAACACAACAAC+TGG + chr8_2:33246233-33246252 Msa1244580:intron 55.0%
AATTGGAGGTGGCAGCCTCA+TGG - chr8_2:33246398-33246417 Msa1244590:intron 55.0%
CTTGGGAGGTTGCATCCATG+AGG + chr8_2:33246416-33246435 Msa1244580:intron 55.0%
CTCATGGATGCAACCTCCCA+AGG - chr8_2:33246414-33246433 Msa1244590:intron 55.0%
TCTTCTCCAGGAGCTGGGAA+TGG - chr8_2:33248071-33248090 Msa1244590:intron 55.0%
GGTTCGTCATATTGGGGCAC+AGG - chr8_2:33248142-33248161 Msa1244590:intron 55.0%
TACCTGGGGTCCCAGATACA+TGG - chr8_2:33248365-33248384 Msa1244590:CDS 55.0%
! TTCCATGTATCTGGGACCCC+AGG + chr8_2:33248370-33248389 None:intergenic 55.0%
GGAGATGCTGATATCGAGCC+AGG + chr8_2:33248892-33248911 None:intergenic 55.0%
GTGAATAAGTGGGGAGTCCG+GGG + chr8_2:33249317-33249336 None:intergenic 55.0%
GGTGAATAAGTGGGGAGTCC+GGG + chr8_2:33249318-33249337 None:intergenic 55.0%
GTTGCCGGAGGATCGCATTG+AGG - chr8_2:33246241-33246260 Msa1244590:intron 60.0%
CCCTCCTCAATGCGATCCTC+CGG + chr8_2:33246248-33246267 Msa1244580:intron 60.0%
CCGGAGGATCGCATTGAGGA+GGG - chr8_2:33246245-33246264 Msa1244590:intron 60.0%
TATGCTCACGGGACCAGCCA+AGG + chr8_2:33247302-33247321 None:intergenic 60.0%
TGGCTTCCATTCCCAGCTCC+TGG + chr8_2:33248080-33248099 None:intergenic 60.0%
AGGAGCTGGGAATGGAAGCC+AGG - chr8_2:33248079-33248098 Msa1244590:intron 60.0%
GCCGGAGGATCGCATTGAGG+AGG - chr8_2:33246244-33246263 Msa1244590:intron 65.0%
GGAGTCCGGGGTTCGAATCC+CGG + chr8_2:33249305-33249324 None:intergenic 65.0%
AGGGGCCGGGATTCGAACCC+CGG - chr8_2:33249297-33249316 Msa1244590:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 33246009 33249537 33246009 ID=Msa1244590;Name=Msa1244590
chr8_2 mRNA 33246009 33249537 33246009 ID=Msa1244590-mRNA-1;Parent=Msa1244590;Name=Msa1244590-mRNA-1;_AED=0.05;_eAED=0.05;_QI=50|1|1|1|0.8|0.66|6|682|417
chr8_2 exon 33246009 33246136 33246009 ID=Msa1244590-mRNA-1:exon:10781;Parent=Msa1244590-mRNA-1
chr8_2 exon 33246554 33247465 33246554 ID=Msa1244590-mRNA-1:exon:10780;Parent=Msa1244590-mRNA-1
chr8_2 exon 33247810 33247956 33247810 ID=Msa1244590-mRNA-1:exon:10779;Parent=Msa1244590-mRNA-1
chr8_2 exon 33248265 33248464 33248265 ID=Msa1244590-mRNA-1:exon:10778;Parent=Msa1244590-mRNA-1
chr8_2 exon 33248786 33248957 33248786 ID=Msa1244590-mRNA-1:exon:10777;Parent=Msa1244590-mRNA-1
chr8_2 exon 33249111 33249537 33249111 ID=Msa1244590-mRNA-1:exon:10776;Parent=Msa1244590-mRNA-1
chr8_2 five_prime_UTR 33249488 33249537 33249488 ID=Msa1244590-mRNA-1:five_prime_utr;Parent=Msa1244590-mRNA-1
chr8_2 CDS 33249111 33249487 33249111 ID=Msa1244590-mRNA-1:cds;Parent=Msa1244590-mRNA-1
chr8_2 CDS 33248786 33248957 33248786 ID=Msa1244590-mRNA-1:cds;Parent=Msa1244590-mRNA-1
chr8_2 CDS 33248265 33248464 33248265 ID=Msa1244590-mRNA-1:cds;Parent=Msa1244590-mRNA-1
chr8_2 CDS 33247810 33247956 33247810 ID=Msa1244590-mRNA-1:cds;Parent=Msa1244590-mRNA-1
chr8_2 CDS 33247108 33247465 33247108 ID=Msa1244590-mRNA-1:cds;Parent=Msa1244590-mRNA-1
chr8_2 three_prime_UTR 33246554 33247107 33246554 ID=Msa1244590-mRNA-1:three_prime_utr;Parent=Msa1244590-mRNA-1
chr8_2 three_prime_UTR 33246009 33246136 33246009 ID=Msa1244590-mRNA-1:three_prime_utr;Parent=Msa1244590-mRNA-1
Gene Sequence

>Msa1244590

ATGCTGTCTCTCAAGTCTTCGATTGATCGCCGGATCCAATCAATTTTCCCAATTATATTGTATTCACTTCTAATCTTTGCAGTTACAAGGTTAGTCCTAGATAGCTTGAAGAGCAAGCAAAGCCATGTTTTTCAGCTCTACGGCATGTCATCTGGTGGAAGGAATCGATTACCAGTTGTTGTGTTGCCGGAGGATCGCATTGAGGAGGGTTGCAATGTATTTGAAGGGAAATGGGTATGGGATAATGTGTCCTATCCTCTTTATGAAGAAGAGAGTTGCCCTTACTTGGTCAAGCAAACCACCTGTAAGAAGAATGGGAGACCTGATTCTTTCTACACAAATTGGAGGTGGCAGCCTCATGGATGCAACCTCCCAAGGTTTGATCCTTTGAAGTTGTTGCACATGTTGAGGAACAAAAGAATGATGTTTATTGGAGACTCATTACAAAGAGGCCAATTTGAGTCTATGATCTGTTTGGTACAATCTGTAATTCCTGAAGGAAAGAAATCCCTCCAAAGAATTCCACCTATGAAGATATTTAGAGTAGAGGAGTTTAATGCATCGATTGAATACTACTGGGCTCCATTCATGGTTGAATCAATTTCTGATCATGCAACAAATCATACGGTACATAAACGGATGGTGATGCTTGACTCAATAGCTAAGCATGGCAAACATTGGCAAGGAGTGGACATTTTGGTGTTCGAGAGTTATGTTTGGTGGATGCACAGCCCTTTCATCAATGCTACATATGGATCACCAGATGAAGTCCAAGAATATAACGTCACGACAGCATATAAGTTGGCCTTAAAAACATGGGCAAATTGGTTAGAATCAAACATCCAACCACTTACTCAAAATGTATTTTTCATGAGTATGTCTCCAACACATTTGTGGAGCTGGGAATGGAAGCCAGGAAGTGATGAAAACTGCTTCAATGAATCATACCCAATACAAGGTTCGTCATATTGGGGCACAGGCTCGAATCTTGAGATTATGAAGATATTACATGATTCACTACAAGAACTGAAAATAGATGTCACCCTATTGAACATCACCCAATTATCAGAGTACAGAAAAGATGCTCACACATCAGTTTACGGCGAGCGAAAGGGCAAGCTCCTGACTAAAGAACAGAGATCTAACCCAAAATCTTTTGCTGATTGCATTCACTGGTGTTTACCTGGGGTCCCAGATACATGGAATGAAATCCTATATGCATATCTGTTTAAGAACTATCAAAACTTTTCTTAA
Protein sequence

>Msa1244590

MLSLKSSIDRRIQSIFPIILYSLLIFAVTRLVLDSLKSKQSHVFQLYGMSSGGRNRLPVVVLPEDRIEEGCNVFEGKWVWDNVSYPLYEEESCPYLVKQTTCKKNGRPDSFYTNWRWQPHGCNLPRFDPLKLLHMLRNKRMMFIGDSLQRGQFESMICLVQSVIPEGKKSLQRIPPMKIFRVEEFNASIEYYWAPFMVESISDHATNHTVHKRMVMLDSIAKHGKHWQGVDILVFESYVWWMHSPFINATYGSPDEVQEYNVTTAYKLALKTWANWLESNIQPLTQNVFFMSMSPTHLWSWEWKPGSDENCFNESYPIQGSSYWGTGSNLEIMKILHDSLQELKIDVTLLNITQLSEYRKDAHTSVYGERKGKLLTKEQRSNPKSFADCIHWCLPGVPDTWNEILYAYLFKNYQNFS*