AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1263010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1263010 MtrunA17_Chr4g0065651 83.112 527 55 9 32 545 7 512 1.69e-137 408
Msa1263010 MtrunA17_Chr0c22g0493881 82.228 377 45 6 32 403 7 366 4.19e-86 271
Msa1263010 MtrunA17_Chr4g0065621 85.315 286 26 4 32 314 7 279 8.00e-82 260
Msa1263010 MtrunA17_Chr2g0287751 52.757 544 171 21 32 545 7 494 2.26e-69 231
Msa1263010 MtrunA17_Chr2g0287741 48.524 542 185 23 30 545 10 483 1.15e-48 177
Msa1263010 MtrunA17_Chr4g0065611 85.714 133 13 2 417 545 12 142 2.16e-39 140
Msa1263010 MtrunA17_Chr0c22g0493871 86.364 132 12 2 418 545 1 130 2.71e-39 139
Msa1263010 MtrunA17_Chr1g0191671 58.915 129 48 3 33 156 8 136 1.16e-28 120
Msa1263010 MtrunA17_Chr1g0208301 69.565 69 21 0 32 100 10 78 4.32e-26 110
Msa1263010 MtrunA17_Chr7g0269081 58.974 78 27 1 22 99 14 86 8.42e-23 99.0
Msa1263010 MtrunA17_Chr4g0067291 38.217 157 71 2 23 153 62 218 2.13e-22 99.0
Msa1263010 MtrunA17_Chr7g0272571 57.576 66 28 0 34 99 32 97 5.29e-19 89.0
Msa1263010 MtrunA17_Chr1g0192131 56.962 79 32 2 25 102 6 83 1.42e-18 88.2
Msa1263010 MtrunA17_Chr3g0111541 54.545 66 30 0 33 98 10 75 3.29e-18 83.6
Msa1263010 MtrunA17_Chr2g0296001 50.000 86 38 1 14 99 57 137 4.95e-16 79.7
Msa1263010 MtrunA17_Chr1g0198341 38.667 75 46 0 34 108 3 77 3.24e-13 65.1
Msa1263010 MtrunA17_Chr8g0382721 52.459 61 29 0 39 99 33 93 1.13e-12 65.9
Msa1263010 MtrunA17_Chr1g0160201 45.902 61 33 0 39 99 29 89 2.31e-12 64.7
Msa1263010 MtrunA17_Chr2g0285561 49.275 69 34 1 37 104 10 78 2.72e-12 67.8
Msa1263010 MtrunA17_Chr7g0251491 34.286 105 61 4 27 123 39 143 4.94e-12 64.7
Msa1263010 MtrunA17_Chr2g0325601 42.254 71 41 0 39 109 6 76 5.52e-12 63.5
Msa1263010 MtrunA17_Chr3g0124411 50.820 61 30 0 39 99 34 94 9.23e-12 63.2
Msa1263010 MtrunA17_Chr7g0218981 36.471 85 54 0 34 118 3 87 9.76e-12 62.4
Msa1263010 MtrunA17_Chr4g0028461 48.276 87 43 2 37 121 68 154 1.21e-11 66.2
Msa1263010 MtrunA17_Chr1g0189221 40.909 66 39 0 39 104 57 122 3.63e-11 62.4
Msa1263010 MtrunA17_Chr5g0407821 38.462 78 47 1 29 105 16 93 4.94e-11 60.8
Msa1263010 MtrunA17_Chr6g0448861 38.356 73 43 2 29 99 23 95 6.13e-11 60.8
Msa1263010 MtrunA17_Chr5g0404641 43.284 67 38 0 35 101 3 69 8.45e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1263010 AT3G06130.2 52.809 267 107 5 33 299 8 255 1.19e-40 150
Msa1263010 AT3G06130.1 52.809 267 107 5 33 299 8 255 2.49e-39 150
Msa1263010 AT5G19090.2 74.468 94 24 0 33 126 8 101 4.06e-37 143
Msa1263010 AT5G19090.3 74.468 94 24 0 33 126 8 101 4.06e-37 143
Msa1263010 AT5G19090.5 74.468 94 24 0 33 126 8 101 1.56e-36 144
Msa1263010 AT5G19090.4 74.468 94 24 0 33 126 8 101 1.56e-36 144
Msa1263010 AT5G19090.1 74.468 94 24 0 33 126 8 101 1.56e-36 144
Msa1263010 AT5G27690.1 57.303 89 33 1 38 126 33 116 3.73e-25 107
Msa1263010 AT3G05220.1 64.706 68 24 0 32 99 7 74 1.62e-23 104
Msa1263010 AT1G23000.1 65.714 70 23 1 39 108 17 85 8.84e-23 100
Msa1263010 AT1G23000.2 65.714 70 23 1 39 108 77 145 2.03e-22 100
Msa1263010 AT5G37860.1 56.716 67 29 0 35 101 10 76 2.35e-19 88.6
Msa1263010 AT1G56210.1 56.000 75 32 1 35 108 40 114 5.08e-19 89.4
Msa1263010 AT1G06330.1 46.875 64 34 0 39 102 6 69 4.38e-13 67.4
Msa1263010 AT1G66240.1 39.130 69 42 0 31 99 29 97 4.43e-12 63.2
Msa1263010 AT3G56891.1 43.750 64 36 0 39 102 20 83 1.73e-11 63.2
Msa1263010 AT4G23882.1 38.462 117 53 5 11 109 48 163 8.65e-11 63.5
Msa1263010 AT3G02960.1 43.284 67 37 1 35 100 126 192 8.80e-11 62.8

Find 178 sgRNAs with CRISPR-Local

Find 310 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTATATGTGTTTATATTT+TGG 0.061931 8_2:+66040595 Msa1263010:three_prime_UTR
CAAGGGAAGGTCACAGTTTC+AGG 0.145406 8_2:+66038893 Msa1263010:CDS
CAAGATCTAGCTCAATTTAA+AGG 0.182916 8_2:+66039238 Msa1263010:CDS
TACTATGTTAATTATGTTCT+TGG 0.208475 8_2:+66040546 Msa1263010:three_prime_UTR
TGCTGCTGCTGTTGTTGATA+TGG 0.227139 8_2:-66040087 None:intergenic
TAGGATTCTGATTCGGGTTC+TGG 0.245506 8_2:-66039291 None:intergenic
GGATTCCCCTGCATCATTTC+CGG 0.253390 8_2:-66040066 None:intergenic
TATTGTTTCCACCACCCTTC+TGG 0.290425 8_2:-66039099 None:intergenic
CATCTTTAGGATTCTGATTC+GGG 0.295568 8_2:-66039297 None:intergenic
ATCAAGAAGCTTGCAAAATC+AGG 0.297726 8_2:+66038938 Msa1263010:CDS
TTAACACCTTTAGCATCTTT+AGG 0.299936 8_2:-66039310 None:intergenic
CCCTGCATCATTTCCGGTCC+TGG 0.305122 8_2:-66040060 None:intergenic
ACAACAACAGCAACAGTTAA+TGG 0.305860 8_2:+66040110 Msa1263010:CDS
AGCTTCTTGATGAGAACATT+TGG 0.309355 8_2:-66038926 None:intergenic
TCAATCTCATCCTGGTAGTA+TGG 0.312405 8_2:+66039915 Msa1263010:CDS
TTGTATTTGTTGACCACCTT+TGG 0.313268 8_2:-66039128 None:intergenic
CCATTCCCCATAGGCATAGA+AGG 0.318053 8_2:-66039472 None:intergenic
TAAGGATCTGGAGGAGGATA+AGG 0.339463 8_2:-66040249 None:intergenic
GCATCTTTAGGATTCTGATT+CGG 0.340947 8_2:-66039298 None:intergenic
ACTGATTAACAACGTTGTTC+TGG 0.344905 8_2:-66039015 None:intergenic
GGCGGAGGTGGAGGCGGTGG+TGG 0.350490 8_2:+66039835 Msa1263010:CDS
GAAATCAATGTTATTGAGAA+TGG 0.356396 8_2:+66040649 Msa1263010:three_prime_UTR
GGGTGGTGGAGGAAATAATA+TGG 0.357181 8_2:+66039936 Msa1263010:CDS
ATAGATGCAGCTAAATTTCC+TGG 0.359801 8_2:+66036779 Msa1263010:CDS
TTACCACCCATTCGGTTCAT+AGG 0.360924 8_2:-66039973 None:intergenic
TTCTATAAACTACTCCTCTA+TGG 0.368659 8_2:-66040507 None:intergenic
CTAAAATCATCCTCATCTTC+AGG 0.371343 8_2:-66039343 None:intergenic
GGTGGCGGAGGTGGAGGCGG+TGG 0.373427 8_2:+66039832 Msa1263010:CDS
TTCTGGCCCGGATGCTGATT+CGG 0.380360 8_2:-66039274 None:intergenic
TACTTTCAAGGTGGTGGTGG+TGG 0.384334 8_2:+66040036 Msa1263010:CDS
GAGAAATGAGCAACCAGAGC+TGG 0.388714 8_2:-66040686 None:intergenic
CCACCATAGCAGCACGTTGT+TGG 0.393632 8_2:-66040149 None:intergenic
AATGGTGGTGGTGGCGGAAA+TGG 0.397465 8_2:+66039559 Msa1263010:CDS
AACAAGAATGGAGGGAATAA+TGG 0.397922 8_2:+66039760 Msa1263010:CDS
CATCAAACAAACAATGTTCA+TGG 0.400654 8_2:+66040323 Msa1263010:three_prime_UTR
CAGGGAAACATGCCGAGTTA+TGG 0.402581 8_2:+66038957 Msa1263010:CDS
CCAATTGGTGGCGGAGGTGG+AGG 0.411942 8_2:+66039826 Msa1263010:CDS
AATGTTCAGGCTATGAATAA+TGG 0.423799 8_2:+66039859 Msa1263010:CDS
GCTTCTTGATGAGAACATTT+GGG 0.423822 8_2:-66038925 None:intergenic
TGTATTTGTTGACCACCTTT+GGG 0.427960 8_2:-66039127 None:intergenic
AAAATCTTACAGAAAATTGA+TGG 0.430085 8_2:+66038698 Msa1263010:CDS
ATGATGGGTGGTGGAGGTGG+TGG 0.432564 8_2:+66039892 Msa1263010:CDS
AAGAAAGGTGGTGGTGGTGG+TGG 0.432913 8_2:+66039655 Msa1263010:CDS
CCTGTGCCGGTTCAAATTCA+TGG 0.435135 8_2:+66039604 Msa1263010:CDS
AAAATGATGGGTGGTGGAGG+TGG 0.436665 8_2:+66039889 Msa1263010:CDS
GGTAACATTCCTGCTGTTCA+AGG 0.436866 8_2:+66039991 Msa1263010:CDS
CTCAAGCCTCAAGGCACAAA+TGG 0.437527 8_2:+66036807 Msa1263010:CDS
AGGCGGTGGTGGTAATGTTC+AGG 0.438058 8_2:+66039846 Msa1263010:CDS
TTCTGATTCGGGTTCTGGCC+CGG 0.438392 8_2:-66039286 None:intergenic
GGTTACTTTCAAGGTGGTGG+TGG 0.439749 8_2:+66040033 Msa1263010:CDS
CCTCCACCTCCGCCACCAAT+TGG 0.440018 8_2:-66039826 None:intergenic
TTCATGCCATGAATTTGAAC+CGG 0.443413 8_2:-66039610 None:intergenic
GGAAAGAAAGGTGGTGGTGG+TGG 0.445640 8_2:+66039652 Msa1263010:CDS
ACTATGTTAATTATGTTCTT+GGG 0.447777 8_2:+66040547 Msa1263010:three_prime_UTR
ATTGGTGGCGGAGGTGGAGG+CGG 0.453929 8_2:+66039829 Msa1263010:CDS
ATACCAATTGGTGGCGGAGG+TGG 0.457299 8_2:+66039823 Msa1263010:CDS
CCTTCTATGCCTATGGGGAA+TGG 0.458052 8_2:+66039472 Msa1263010:CDS
TGATGAGTTTGATGATGAAA+TGG 0.461887 8_2:+66039420 Msa1263010:CDS
AACAACTTCAACAGATGAAA+GGG 0.462139 8_2:+66039212 Msa1263010:CDS
CGGTTCATAGGCATACTCAT+TGG 0.463995 8_2:-66039961 None:intergenic
ACAGCTTATGAGTGCTATGA+AGG 0.465021 8_2:+66039525 Msa1263010:CDS
AAGGATCTGGAGGAGGATAA+GGG 0.466318 8_2:-66040248 None:intergenic
GGTCTACCGGCAGGCGGAGG+AGG 0.468185 8_2:+66040012 Msa1263010:CDS
AAAGCATGTGAGTAAGGATC+TGG 0.469198 8_2:-66040261 None:intergenic
CATGGCATGAATGGTGGAAA+TGG 0.469268 8_2:+66039622 Msa1263010:CDS
TGGTGGTGGTGGTCCAGGAC+CGG 0.469917 8_2:+66040047 Msa1263010:CDS
GATGCCGCCGAATCAGCATC+CGG 0.474547 8_2:+66039267 Msa1263010:CDS
AAATCAATGTTATTGAGAAT+GGG 0.477281 8_2:+66040650 Msa1263010:three_prime_UTR
TCCAGGACCGGAAATGATGC+AGG 0.480211 8_2:+66040059 Msa1263010:CDS
GGCGGAGGAGGTTACTTTCA+AGG 0.482444 8_2:+66040024 Msa1263010:CDS
CAATCTCATCCTGGTAGTAT+GGG 0.490190 8_2:+66039916 Msa1263010:CDS
TCGCTGAAAGCATGTGAGTA+AGG 0.494235 8_2:-66040267 None:intergenic
AAGGGTATGTCGATACCAAT+TGG 0.495826 8_2:+66039811 Msa1263010:CDS
ATGAAAGAAATTCCCAGCTC+TGG 0.499167 8_2:+66040673 Msa1263010:three_prime_UTR
TCCTGCTGTTCAAGGTCTAC+CGG 0.501026 8_2:+66039999 Msa1263010:CDS
GCCGGTAGACCTTGAACAGC+AGG 0.503563 8_2:-66040000 None:intergenic
GGTGGCCTTGCATACATCAT+TGG 0.504586 8_2:-66040195 None:intergenic
TCTGGAGGAGGATAAGGGTA+AGG 0.506412 8_2:-66040243 None:intergenic
ATTGACAATGTCAAAGGTGG+TGG 0.509050 8_2:+66039052 Msa1263010:CDS
CATCCTGGTAGTATGGGTGG+TGG 0.513520 8_2:+66039922 Msa1263010:CDS
AATGGTGGTGGAAACAAGAA+TGG 0.515647 8_2:+66039748 Msa1263010:CDS
CCATGAATTTGAACCGGCAC+AGG 0.516132 8_2:-66039604 None:intergenic
GGAGGTTACTTTCAAGGTGG+TGG 0.516276 8_2:+66040030 Msa1263010:CDS
GGTGGTGGAGGAAATAATAT+GGG 0.517548 8_2:+66039937 Msa1263010:CDS
AAGTAACCTCCTCCGCCTGC+CGG 0.520732 8_2:-66040018 None:intergenic
GTAGTAATCAAAATAACGAT+GGG 0.520911 8_2:+66039782 Msa1263010:CDS
CAGAATCCTAAAGATGCTAA+AGG 0.521196 8_2:+66039304 Msa1263010:CDS
AATCAAAACCAGAAGGGTGG+TGG 0.522342 8_2:+66039091 Msa1263010:CDS
GGAGGAAAGAAAGGTGGTGG+TGG 0.522847 8_2:+66039649 Msa1263010:CDS
CCAACAACGTGCTGCTATGG+TGG 0.525200 8_2:+66040149 Msa1263010:CDS
CAGGAATGTTACCACCCATT+CGG 0.525275 8_2:-66039981 None:intergenic
GGTGCTCACATGATGTTGAA+TGG 0.526053 8_2:+66039493 Msa1263010:CDS
GTTCAAATTCATGGCATGAA+TGG 0.526661 8_2:+66039613 Msa1263010:CDS
CAACAACTTCAACAGATGAA+AGG 0.526664 8_2:+66039211 Msa1263010:CDS
CTTGAGGCTTGAGCGATGCC+AGG 0.526844 8_2:-66036797 None:intergenic
CAAGGTCTACCGGCAGGCGG+AGG 0.530336 8_2:+66040009 Msa1263010:CDS
ATATAATTCACCTCAGGAGG+TGG 0.531915 8_2:-66040213 None:intergenic
AATGGCAATGGAGGAAAGAA+AGG 0.535264 8_2:+66039640 Msa1263010:CDS
GGACAGAAACCTGGTGGTGG+AGG 0.535285 8_2:+66039580 Msa1263010:CDS
CCTGGTAGTATGGGTGGTGG+AGG 0.540462 8_2:+66039925 Msa1263010:CDS
GTGCTCACATGATGTTGAAT+GGG 0.541270 8_2:+66039494 Msa1263010:CDS
TCATTGGCTGCTGAAATCTC+TGG 0.541514 8_2:-66040179 None:intergenic
GCTGTTCAAGGTCTACCGGC+AGG 0.542942 8_2:+66040003 Msa1263010:CDS
GAATCACATGACATTGCAGC+TGG 0.546046 8_2:-66040301 None:intergenic
GGAGGAGGATAAGGGTAAGG+AGG 0.547967 8_2:-66040240 None:intergenic
CCTCCACCACCCATACTACC+AGG 0.550212 8_2:-66039925 None:intergenic
TCAAGAAGCTTGCAAAATCA+GGG 0.551852 8_2:+66038939 Msa1263010:CDS
GGCGGAAATGGACAGAAACC+TGG 0.552019 8_2:+66039571 Msa1263010:CDS
TCGATACCAATTGGTGGCGG+AGG 0.554646 8_2:+66039820 Msa1263010:CDS
TGGTGGTGGAGGACCTGTGC+CGG 0.556236 8_2:+66039591 Msa1263010:CDS
GAACCAACAACGTGCTGCTA+TGG 0.559788 8_2:+66040146 Msa1263010:CDS
ATGCAAATTGACAATGTCAA+AGG 0.561214 8_2:+66039046 Msa1263010:CDS
GGTGGTGGTCAATCTCATCC+TGG 0.561328 8_2:+66039907 Msa1263010:CDS
AGAAATGAGCAACCAGAGCT+GGG 0.565461 8_2:-66040685 None:intergenic
ATGCCGCCGAATCAGCATCC+GGG 0.568311 8_2:+66039268 Msa1263010:CDS
CTGGCATCGCTCAAGCCTCA+AGG 0.569109 8_2:+66036798 Msa1263010:CDS
GGTGGTGGAAACAAGAATGG+AGG 0.570437 8_2:+66039751 Msa1263010:CDS
CAAATTCATGGCATGAATGG+TGG 0.570734 8_2:+66039616 Msa1263010:CDS
CATTCCCCATAGGCATAGAA+GGG 0.572536 8_2:-66039471 None:intergenic
TTTGTTGACCACCTTTGGGT+TGG 0.576873 8_2:-66039123 None:intergenic
GGCACAGGTCCTCCACCACC+AGG 0.577295 8_2:-66039589 None:intergenic
GAGTTTGATGATGAAATGGT+TGG 0.582338 8_2:+66039424 Msa1263010:CDS
GGTAGTAATCAAAATAACGA+TGG 0.583746 8_2:+66039781 Msa1263010:CDS
GGTCACAGTTTCAGGGAATG+TGG 0.584262 8_2:+66038901 Msa1263010:CDS
AGCAGCCAATGATGTATGCA+AGG 0.586173 8_2:+66040190 Msa1263010:CDS
ACAAACAATGTTCATGGTGA+TGG 0.588045 8_2:+66040329 Msa1263010:three_prime_UTR
ATGAATGGTGGAAATGGCAA+TGG 0.589322 8_2:+66039628 Msa1263010:CDS
GGATACATATAATTCACCTC+AGG 0.589585 8_2:-66040219 None:intergenic
AAGGGAAGGTCACAGTTTCA+GGG 0.589814 8_2:+66038894 Msa1263010:CDS
GGGGTTTCTAATTACCATAG+AGG 0.591890 8_2:+66040493 Msa1263010:three_prime_UTR
GTGGTGGAAACAAGAATGGA+GGG 0.595867 8_2:+66039752 Msa1263010:CDS
CAACAAATACAACAACTACA+AGG 0.597690 8_2:+66039139 Msa1263010:CDS
AGGTCAGAATCAAAACCAGA+AGG 0.597938 8_2:+66039084 Msa1263010:CDS
AATGGACAGAAACCTGGTGG+TGG 0.598210 8_2:+66039577 Msa1263010:CDS
TTTGGGAACACTCCATAACT+CGG 0.598490 8_2:-66038969 None:intergenic
ATTTGATGTGCCTGAAGATG+AGG 0.599659 8_2:+66039333 Msa1263010:CDS
GGCAATGGAGGAAAGAAAGG+TGG 0.603629 8_2:+66039643 Msa1263010:CDS
TGTGAGTAAGGATCTGGAGG+AGG 0.603879 8_2:-66040255 None:intergenic
AACAATAACCAACCCAAAGG+TGG 0.605261 8_2:+66039115 Msa1263010:CDS
AATGGAGGAAAGAAAGGTGG+TGG 0.606743 8_2:+66039646 Msa1263010:CDS
AATGTTCATGGTGATGGTGA+TGG 0.608623 8_2:+66040335 Msa1263010:three_prime_UTR
TTCATAGCACTCATAAGCTG+TGG 0.610940 8_2:-66039523 None:intergenic
TCTCATCCTGGTAGTATGGG+TGG 0.611507 8_2:+66039919 Msa1263010:CDS
TACATATAATTCACCTCAGG+AGG 0.615331 8_2:-66040216 None:intergenic
AAAGTGAACATACACTGTGA+TGG 0.617847 8_2:+66038656 Msa1263010:CDS
CCAGGACCGGAAATGATGCA+GGG 0.618163 8_2:+66040060 Msa1263010:CDS
ATGTGAGCACCATTCCCCAT+AGG 0.619841 8_2:-66039481 None:intergenic
ACTGAGATAGATGCAGAACA+AGG 0.622005 8_2:+66038875 Msa1263010:CDS
AGTATGCCTATGAACCGAAT+GGG 0.622246 8_2:+66039967 Msa1263010:CDS
AATAATCAGAATCAAGGTGG+AGG 0.625159 8_2:+66039688 Msa1263010:CDS
GGTCAGAATCAAAACCAGAA+GGG 0.626233 8_2:+66039085 Msa1263010:CDS
ATGTCGATACCAATTGGTGG+CGG 0.631785 8_2:+66039817 Msa1263010:CDS
GTTCAAGGTCTACCGGCAGG+CGG 0.633909 8_2:+66040006 Msa1263010:CDS
GCATGTGAGTAAGGATCTGG+AGG 0.633919 8_2:-66040258 None:intergenic
GGTATGTCGATACCAATTGG+TGG 0.637679 8_2:+66039814 Msa1263010:CDS
CTTATGAGTGCTATGAAGGG+TGG 0.638762 8_2:+66039529 Msa1263010:CDS
GATAGATGCAGAACAAGGGA+AGG 0.641999 8_2:+66038880 Msa1263010:CDS
CAGCTTATGAGTGCTATGAA+GGG 0.642847 8_2:+66039526 Msa1263010:CDS
TAGTAATCAAAATAACGATG+GGG 0.650397 8_2:+66039783 Msa1263010:CDS
ATGCCTATGAACCGAATGGG+TGG 0.650716 8_2:+66039970 Msa1263010:CDS
GAGTATGCCTATGAACCGAA+TGG 0.653536 8_2:+66039966 Msa1263010:CDS
TGGCCCGGATGCTGATTCGG+CGG 0.659918 8_2:-66039271 None:intergenic
AGTAATCAAAATAACGATGG+GGG 0.662461 8_2:+66039784 Msa1263010:CDS
AATGGTGGAAATGGCAATGG+AGG 0.665518 8_2:+66039631 Msa1263010:CDS
CTGAGATAGATGCAGAACAA+GGG 0.666808 8_2:+66038876 Msa1263010:CDS
CAGGACCGGAAATGATGCAG+GGG 0.670762 8_2:+66040061 Msa1263010:CDS
TGCATAAAATTAGAGAGCAT+AGG 0.671554 8_2:-66040391 None:intergenic
ATTCCCCATAGGCATAGAAG+GGG 0.672578 8_2:-66039470 None:intergenic
CAAATTGACAATGTCAAAGG+TGG 0.676115 8_2:+66039049 Msa1263010:CDS
AAAGGTGGTGGAAACAACAA+AGG 0.677851 8_2:+66039064 Msa1263010:CDS
CAGAATCAAAACCAGAAGGG+TGG 0.682186 8_2:+66039088 Msa1263010:CDS
GGAAACAATAACCAACCCAA+AGG 0.682874 8_2:+66039112 Msa1263010:CDS
GTATGCAAGGCCACCTCCTG+AGG 0.686248 8_2:+66040203 Msa1263010:CDS
GGGAATAATCAGAATCAAGG+TGG 0.692585 8_2:+66039685 Msa1263010:CDS
TTCCCCATAGGCATAGAAGG+GGG 0.698362 8_2:-66039469 None:intergenic
GTGGTGGAGGAAATAATATG+GGG 0.726893 8_2:+66039938 Msa1263010:CDS
GGAAATGGACAGAAACCTGG+TGG 0.762212 8_2:+66039574 Msa1263010:CDS
GGAGGAGGTTACTTTCAAGG+TGG 0.764262 8_2:+66040027 Msa1263010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTAAAATTAAAAAT+TGG + chr8_2:66037238-66037257 Msa1263010:intron 0.0%
!!! AGTTTTTTAAATTTTTTTAG+CGG + chr8_2:66037675-66037694 Msa1263010:intron 10.0%
!!! CAATAAAACTTTTATTTTTT+CGG - chr8_2:66037763-66037782 None:intergenic 10.0%
!!! TTATTATTTTGATTATTGTT+TGG - chr8_2:66038995-66039014 None:intergenic 10.0%
!!! TTTATATGTGTTTATATTTT+GGG + chr8_2:66040596-66040615 Msa1263010:three_prime_UTR 10.0%
!! AATAATACATAAAATATGCA+AGG + chr8_2:66037514-66037533 Msa1263010:intron 15.0%
!!! GTTTATATGTGTTTATATTT+TGG + chr8_2:66040595-66040614 Msa1263010:three_prime_UTR 15.0%
!!! ATTTTTTTTGTCAAGTAACA+TGG + chr8_2:66037346-66037365 Msa1263010:intron 20.0%
!!! GAACTTACATATTTTAAATG+TGG + chr8_2:66037375-66037394 Msa1263010:intron 20.0%
!! AGAAAAATAGGGTTAAATTA+AGG + chr8_2:66038040-66038059 Msa1263010:intron 20.0%
!! AGCAAAAAAATAAAGAAAGA+TGG - chr8_2:66038532-66038551 None:intergenic 20.0%
!! AAAATCTTACAGAAAATTGA+TGG + chr8_2:66038698-66038717 Msa1263010:CDS 20.0%
!! ATAATTGAAAGCTATGATTA+AGG - chr8_2:66038764-66038783 None:intergenic 20.0%
!! AACATTTACTCTCAAAATTA+AGG - chr8_2:66038810-66038829 None:intergenic 20.0%
!!! TTTTGATTATTGTTTGGTTT+GGG - chr8_2:66038989-66039008 None:intergenic 20.0%
!!! ATTTTGATTATTGTTTGGTT+TGG - chr8_2:66038990-66039009 None:intergenic 20.0%
!!! TAATGGATTTCAAAAAATGA+TGG + chr8_2:66039876-66039895 Msa1263010:CDS 20.0%
!!! AATGGATTTCAAAAAATGAT+GGG + chr8_2:66039877-66039896 Msa1263010:CDS 20.0%
!! TACTATGTTAATTATGTTCT+TGG + chr8_2:66040546-66040565 Msa1263010:three_prime_UTR 20.0%
!! ACTATGTTAATTATGTTCTT+GGG + chr8_2:66040547-66040566 Msa1263010:three_prime_UTR 20.0%
!! AAATCAATGTTATTGAGAAT+GGG + chr8_2:66040650-66040669 Msa1263010:three_prime_UTR 20.0%
! AGAAAAATGAGAAAGTTTCT+GGG + chr8_2:66036753-66036772 Msa1263010:CDS 25.0%
! AATAGTAGTAACATCTATTG+AGG - chr8_2:66036924-66036943 None:intergenic 25.0%
! GATATTGCATATTACATGTA+GGG - chr8_2:66037433-66037452 None:intergenic 25.0%
! TACATAAAATATGCAAGGTA+TGG + chr8_2:66037519-66037538 Msa1263010:intron 25.0%
! ACATAAAATATGCAAGGTAT+GGG + chr8_2:66037520-66037539 Msa1263010:intron 25.0%
! TAGAAAGAGAGAAGAAAAAT+AGG + chr8_2:66038028-66038047 Msa1263010:intron 25.0%
! AGAAAGAGAGAAGAAAAATA+GGG + chr8_2:66038029-66038048 Msa1263010:intron 25.0%
! AAAATAGGGTTAAATTAAGG+TGG + chr8_2:66038043-66038062 Msa1263010:intron 25.0%
!!! TGTTTGGTGTCTTTATTTAT+TGG - chr8_2:66038171-66038190 None:intergenic 25.0%
! AAGCAAATGAGAGAAATAAA+TGG - chr8_2:66038309-66038328 None:intergenic 25.0%
!!! TATTTTATGTTCTTGTTTGG+TGG - chr8_2:66038371-66038390 None:intergenic 25.0%
!! GAGTATTTTATGTTCTTGTT+TGG - chr8_2:66038374-66038393 None:intergenic 25.0%
! CTTGTGATAAATAAAGATCT+GGG - chr8_2:66038433-66038452 None:intergenic 25.0%
! ACTTGTGATAAATAAAGATC+TGG - chr8_2:66038434-66038453 None:intergenic 25.0%
! GTTACTTACTTTATTCCTAT+TGG + chr8_2:66038452-66038471 Msa1263010:intron 25.0%
!!! AGAAGAATTTTTGAAGATAC+AGG + chr8_2:66038485-66038504 Msa1263010:intron 25.0%
!! TCAAAATTAAGGTTCTGTTT+TGG - chr8_2:66038799-66038818 None:intergenic 25.0%
!!! TGATTTTAGTGATGATGAAA+TGG + chr8_2:66039357-66039376 Msa1263010:CDS 25.0%
! GTAGTAATCAAAATAACGAT+GGG + chr8_2:66039782-66039801 Msa1263010:CDS 25.0%
! TAGTAATCAAAATAACGATG+GGG + chr8_2:66039783-66039802 Msa1263010:CDS 25.0%
! TAAAAAACTCATGTTTCACT+GGG - chr8_2:66040427-66040446 None:intergenic 25.0%
! GAAATCAATGTTATTGAGAA+TGG + chr8_2:66040649-66040668 Msa1263010:three_prime_UTR 25.0%
! TTTTTCTGATCCTTCTTTTC+TGG - chr8_2:66036740-66036759 None:intergenic 30.0%
CAGAAAAATGAGAAAGTTTC+TGG + chr8_2:66036752-66036771 Msa1263010:CDS 30.0%
!!! GCTTGATGTCACTTTTAAAA+GGG + chr8_2:66036956-66036975 Msa1263010:intron 30.0%
!!! TTTTTTGTCAAGTAACATGG+TGG + chr8_2:66037349-66037368 Msa1263010:intron 30.0%
GGATATTGCATATTACATGT+AGG - chr8_2:66037434-66037453 None:intergenic 30.0%
CATAAAATATGCAAGGTATG+GGG + chr8_2:66037521-66037540 Msa1263010:intron 30.0%
GTCTAAATAATATTTCCGTG+AGG - chr8_2:66037571-66037590 None:intergenic 30.0%
!!! TTTTTTCAGCAATTTAACCG+TGG - chr8_2:66038100-66038119 None:intergenic 30.0%
!!! TTGTTTGGTGGAGTTTTATT+TGG - chr8_2:66038359-66038378 None:intergenic 30.0%
TTCTGCATACACAAATAACA+AGG - chr8_2:66038626-66038645 None:intergenic 30.0%
!! TGTGTTGATTAATGAATGAC+AGG + chr8_2:66038842-66038861 Msa1263010:intron 30.0%
ATGCAAATTGACAATGTCAA+AGG + chr8_2:66039046-66039065 Msa1263010:CDS 30.0%
CAACAAATACAACAACTACA+AGG + chr8_2:66039139-66039158 Msa1263010:CDS 30.0%
AACAACTTCAACAGATGAAA+GGG + chr8_2:66039212-66039231 Msa1263010:CDS 30.0%
! CAAGATCTAGCTCAATTTAA+AGG + chr8_2:66039238-66039257 Msa1263010:CDS 30.0%
TTAACACCTTTAGCATCTTT+AGG - chr8_2:66039313-66039332 None:intergenic 30.0%
!! TGATGAGTTTGATGATGAAA+TGG + chr8_2:66039420-66039439 Msa1263010:CDS 30.0%
ATGCCAGAAGCTAAAAATAA+TGG + chr8_2:66039730-66039749 Msa1263010:CDS 30.0%
GGTAGTAATCAAAATAACGA+TGG + chr8_2:66039781-66039800 Msa1263010:CDS 30.0%
AGTAATCAAAATAACGATGG+GGG + chr8_2:66039784-66039803 Msa1263010:CDS 30.0%
AATGTTCAGGCTATGAATAA+TGG + chr8_2:66039859-66039878 Msa1263010:CDS 30.0%
CATCAAACAAACAATGTTCA+TGG + chr8_2:66040323-66040342 Msa1263010:three_prime_UTR 30.0%
! TGCATAAAATTAGAGAGCAT+AGG - chr8_2:66040394-66040413 None:intergenic 30.0%
CTAAAAAACTCATGTTTCAC+TGG - chr8_2:66040428-66040447 None:intergenic 30.0%
TTCTATAAACTACTCCTCTA+TGG - chr8_2:66040510-66040529 None:intergenic 30.0%
ATAGATGCAGCTAAATTTCC+TGG + chr8_2:66036779-66036798 Msa1263010:CDS 35.0%
AGTAACATCTATTGAGGTTG+TGG - chr8_2:66036918-66036937 None:intergenic 35.0%
!!! CGCTTGATGTCACTTTTAAA+AGG + chr8_2:66036955-66036974 Msa1263010:intron 35.0%
! GGTGAAATTGATTCTAAGAG+TGG + chr8_2:66036977-66036996 Msa1263010:intron 35.0%
CAAGTGATTATGTGTACGTT+TGG + chr8_2:66037001-66037020 Msa1263010:intron 35.0%
GATTATGTGTACGTTTGGTT+CGG + chr8_2:66037006-66037025 Msa1263010:intron 35.0%
CATATTACATGTAGGGATCA+GGG - chr8_2:66037426-66037445 None:intergenic 35.0%
TGAGGTTAACTCAACTGATA+AGG - chr8_2:66037455-66037474 None:intergenic 35.0%
!! ATGATGACGATATTTTCGTG+AGG - chr8_2:66037473-66037492 None:intergenic 35.0%
!!! TTTCCGTGAGGTTTTTTTTG+TGG - chr8_2:66037559-66037578 None:intergenic 35.0%
AAATCACAATGATGCAAGAG+TGG - chr8_2:66037945-66037964 None:intergenic 35.0%
!! AAGAGTCTCTGAAACATAAG+AGG - chr8_2:66038281-66038300 None:intergenic 35.0%
GGCGATTATAGATCTGAAAA+TGG - chr8_2:66038396-66038415 None:intergenic 35.0%
ATCTGGGCTATAGAGAAAAA+TGG - chr8_2:66038417-66038436 None:intergenic 35.0%
CTTCTTTACTCATGACCAAT+AGG - chr8_2:66038470-66038489 None:intergenic 35.0%
AGAAAGATGGAGAATGTGAT+GGG - chr8_2:66038519-66038538 None:intergenic 35.0%
AAGAAAGATGGAGAATGTGA+TGG - chr8_2:66038520-66038539 None:intergenic 35.0%
AAAGTGAACATACACTGTGA+TGG + chr8_2:66038656-66038675 Msa1263010:CDS 35.0%
GCTTCTTGATGAGAACATTT+GGG - chr8_2:66038928-66038947 None:intergenic 35.0%
AGCTTCTTGATGAGAACATT+TGG - chr8_2:66038929-66038948 None:intergenic 35.0%
ATCAAGAAGCTTGCAAAATC+AGG + chr8_2:66038938-66038957 Msa1263010:CDS 35.0%
TCAAGAAGCTTGCAAAATCA+GGG + chr8_2:66038939-66038958 Msa1263010:CDS 35.0%
ACTGATTAACAACGTTGTTC+TGG - chr8_2:66039018-66039037 None:intergenic 35.0%
CAAATTGACAATGTCAAAGG+TGG + chr8_2:66039049-66039068 Msa1263010:CDS 35.0%
! TGTATTTGTTGACCACCTTT+GGG - chr8_2:66039130-66039149 None:intergenic 35.0%
! TTGTATTTGTTGACCACCTT+TGG - chr8_2:66039131-66039150 None:intergenic 35.0%
CAACAACTTCAACAGATGAA+AGG + chr8_2:66039211-66039230 Msa1263010:CDS 35.0%
!! CATCTTTAGGATTCTGATTC+GGG - chr8_2:66039300-66039319 None:intergenic 35.0%
!! GCATCTTTAGGATTCTGATT+CGG - chr8_2:66039301-66039320 None:intergenic 35.0%
CAGAATCCTAAAGATGCTAA+AGG + chr8_2:66039304-66039323 Msa1263010:CDS 35.0%
CTAAAATCATCCTCATCTTC+AGG - chr8_2:66039346-66039365 None:intergenic 35.0%
!! GAGTTTGATGATGAAATGGT+TGG + chr8_2:66039424-66039443 Msa1263010:CDS 35.0%
! ATGAAGGGTGGCAATTTTAA+TGG + chr8_2:66039541-66039560 Msa1263010:CDS 35.0%
TTCATGCCATGAATTTGAAC+CGG - chr8_2:66039613-66039632 None:intergenic 35.0%
GTTCAAATTCATGGCATGAA+TGG + chr8_2:66039613-66039632 Msa1263010:CDS 35.0%
AATAATCAGAATCAAGGTGG+AGG + chr8_2:66039688-66039707 Msa1263010:CDS 35.0%
GGTGGAGGTAACAAAAATAA+TGG + chr8_2:66039703-66039722 Msa1263010:CDS 35.0%
GGAGGTAACAAAAATAATGG+TGG + chr8_2:66039706-66039725 Msa1263010:CDS 35.0%
!!! CCACCATTATTTTTAGCTTC+TGG - chr8_2:66039736-66039755 None:intergenic 35.0%
CCAGAAGCTAAAAATAATGG+TGG + chr8_2:66039733-66039752 Msa1263010:CDS 35.0%
GAAGCTAAAAATAATGGTGG+TGG + chr8_2:66039736-66039755 Msa1263010:CDS 35.0%
AACAAGAATGGAGGGAATAA+TGG + chr8_2:66039760-66039779 Msa1263010:CDS 35.0%
! GGATTTCAAAAAATGATGGG+TGG + chr8_2:66039880-66039899 Msa1263010:CDS 35.0%
!! TTTCAAAAAATGATGGGTGG+TGG + chr8_2:66039883-66039902 Msa1263010:CDS 35.0%
ACAACAACAGCAACAGTTAA+TGG + chr8_2:66040110-66040129 Msa1263010:CDS 35.0%
TACATATAATTCACCTCAGG+AGG - chr8_2:66040219-66040238 None:intergenic 35.0%
GGATACATATAATTCACCTC+AGG - chr8_2:66040222-66040241 None:intergenic 35.0%
ACAAACAATGTTCATGGTGA+TGG + chr8_2:66040329-66040348 Msa1263010:three_prime_UTR 35.0%
! ACTTTTCCATTTGTGCCTTG+AGG - chr8_2:66036816-66036835 None:intergenic 40.0%
GCATATTACATGTAGGGATC+AGG - chr8_2:66037427-66037446 None:intergenic 40.0%
TCTCGAGTTTGTGTCTATCT+CGG + chr8_2:66037601-66037620 Msa1263010:intron 40.0%
GGAGAGAGAAAATATGTGAG+AGG - chr8_2:66037918-66037937 None:intergenic 40.0%
CTAAGCCAATTGAAGTCACA+TGG + chr8_2:66038074-66038093 Msa1263010:intron 40.0%
AATGAAGTCACTCACTTCTG+TGG + chr8_2:66038131-66038150 Msa1263010:intron 40.0%
! CCAAGAGTTTGCTCTATGTT+TGG - chr8_2:66038187-66038206 None:intergenic 40.0%
CCAAACATAGAGCAAACTCT+TGG + chr8_2:66038184-66038203 Msa1263010:intron 40.0%
AGTAGTCACTGAGAGAGAAT+GGG - chr8_2:66038219-66038238 None:intergenic 40.0%
TAGTAGTCACTGAGAGAGAA+TGG - chr8_2:66038220-66038239 None:intergenic 40.0%
TTAGGAAGAGAGAGAATGGT+GGG - chr8_2:66038246-66038265 None:intergenic 40.0%
AGAGTTAGGAAGAGAGAGAA+TGG - chr8_2:66038250-66038269 None:intergenic 40.0%
AAGAGGCAAGTTTGAGAGTT+AGG - chr8_2:66038264-66038283 None:intergenic 40.0%
ACTGAGATAGATGCAGAACA+AGG + chr8_2:66038875-66038894 Msa1263010:CDS 40.0%
CTGAGATAGATGCAGAACAA+GGG + chr8_2:66038876-66038895 Msa1263010:CDS 40.0%
TTTGGGAACACTCCATAACT+CGG - chr8_2:66038972-66038991 None:intergenic 40.0%
ATTGACAATGTCAAAGGTGG+TGG + chr8_2:66039052-66039071 Msa1263010:CDS 40.0%
!! AAAGGTGGTGGAAACAACAA+AGG + chr8_2:66039064-66039083 Msa1263010:CDS 40.0%
AGGTCAGAATCAAAACCAGA+AGG + chr8_2:66039084-66039103 Msa1263010:CDS 40.0%
GGTCAGAATCAAAACCAGAA+GGG + chr8_2:66039085-66039104 Msa1263010:CDS 40.0%
GGAAACAATAACCAACCCAA+AGG + chr8_2:66039112-66039131 Msa1263010:CDS 40.0%
AACAATAACCAACCCAAAGG+TGG + chr8_2:66039115-66039134 Msa1263010:CDS 40.0%
ATTTGATGTGCCTGAAGATG+AGG + chr8_2:66039333-66039352 Msa1263010:CDS 40.0%
GAAGGGGGTTTCATCTTATT+AGG - chr8_2:66039457-66039476 None:intergenic 40.0%
GTGCTCACATGATGTTGAAT+GGG + chr8_2:66039494-66039513 Msa1263010:CDS 40.0%
TTCATAGCACTCATAAGCTG+TGG - chr8_2:66039526-66039545 None:intergenic 40.0%
ACAGCTTATGAGTGCTATGA+AGG + chr8_2:66039525-66039544 Msa1263010:CDS 40.0%
CAGCTTATGAGTGCTATGAA+GGG + chr8_2:66039526-66039545 Msa1263010:CDS 40.0%
!! AAGGGTGGCAATTTTAATGG+TGG + chr8_2:66039544-66039563 Msa1263010:CDS 40.0%
!!! AATTTTAATGGTGGTGGTGG+CGG + chr8_2:66039553-66039572 Msa1263010:CDS 40.0%
CAAATTCATGGCATGAATGG+TGG + chr8_2:66039616-66039635 Msa1263010:CDS 40.0%
! ATGAATGGTGGAAATGGCAA+TGG + chr8_2:66039628-66039647 Msa1263010:CDS 40.0%
AATGGCAATGGAGGAAAGAA+AGG + chr8_2:66039640-66039659 Msa1263010:CDS 40.0%
GGGAATAATCAGAATCAAGG+TGG + chr8_2:66039685-66039704 Msa1263010:CDS 40.0%
!! AATGGTGGTGGAAACAAGAA+TGG + chr8_2:66039748-66039767 Msa1263010:CDS 40.0%
AAATAACGATGGGGGTAAGA+AGG + chr8_2:66039792-66039811 Msa1263010:CDS 40.0%
AATAACGATGGGGGTAAGAA+GGG + chr8_2:66039793-66039812 Msa1263010:CDS 40.0%
! AAGGGTATGTCGATACCAAT+TGG + chr8_2:66039811-66039830 Msa1263010:CDS 40.0%
TCAATCTCATCCTGGTAGTA+TGG + chr8_2:66039915-66039934 Msa1263010:CDS 40.0%
CAATCTCATCCTGGTAGTAT+GGG + chr8_2:66039916-66039935 Msa1263010:CDS 40.0%
!! GGTGGTGGAGGAAATAATAT+GGG + chr8_2:66039937-66039956 Msa1263010:CDS 40.0%
! GTGGTGGAGGAAATAATATG+GGG + chr8_2:66039938-66039957 Msa1263010:CDS 40.0%
AGTATGCCTATGAACCGAAT+GGG + chr8_2:66039967-66039986 Msa1263010:CDS 40.0%
ATATAATTCACCTCAGGAGG+TGG - chr8_2:66040216-66040235 None:intergenic 40.0%
AAAGCATGTGAGTAAGGATC+TGG - chr8_2:66040264-66040283 None:intergenic 40.0%
!! AATGTTCATGGTGATGGTGA+TGG + chr8_2:66040335-66040354 Msa1263010:three_prime_UTR 40.0%
! TTTGACCCCCTTTTCATCTT+AGG + chr8_2:66040471-66040490 Msa1263010:three_prime_UTR 40.0%
! TTGACCCCCTTTTCATCTTA+GGG + chr8_2:66040472-66040491 Msa1263010:three_prime_UTR 40.0%
AAACCCCCTAAGATGAAAAG+GGG - chr8_2:66040480-66040499 None:intergenic 40.0%
GAAACCCCCTAAGATGAAAA+GGG - chr8_2:66040481-66040500 None:intergenic 40.0%
AGAAACCCCCTAAGATGAAA+AGG - chr8_2:66040482-66040501 None:intergenic 40.0%
GGGGTTTCTAATTACCATAG+AGG + chr8_2:66040493-66040512 Msa1263010:three_prime_UTR 40.0%
ATGAAAGAAATTCCCAGCTC+TGG + chr8_2:66040673-66040692 Msa1263010:three_prime_UTR 40.0%
TCGTCATCATGAGCTTAGCT+CGG + chr8_2:66037483-66037502 Msa1263010:intron 45.0%
!!! TGAGGTTTTTTTTGTGGCCG+AGG - chr8_2:66037553-66037572 None:intergenic 45.0%
CGGCCACAAAAAAAACCTCA+CGG + chr8_2:66037553-66037572 Msa1263010:intron 45.0%
CTCGAGTTTGTGTCTATCTC+GGG + chr8_2:66037602-66037621 Msa1263010:intron 45.0%
!! GAAGTGCTACTTGCTAGAAG+GGG - chr8_2:66037896-66037915 None:intergenic 45.0%
!! GGAAGTGCTACTTGCTAGAA+GGG - chr8_2:66037897-66037916 None:intergenic 45.0%
!! AGGAAGTGCTACTTGCTAGA+AGG - chr8_2:66037898-66037917 None:intergenic 45.0%
TCACAATGATGCAAGAGTGG+AGG - chr8_2:66037942-66037961 None:intergenic 45.0%
TGACTTGAAGACTGTGAGGT+GGG - chr8_2:66037987-66038006 None:intergenic 45.0%
! TTGACTTGAAGACTGTGAGG+TGG - chr8_2:66037988-66038007 None:intergenic 45.0%
! AGCTTGACTTGAAGACTGTG+AGG - chr8_2:66037991-66038010 None:intergenic 45.0%
CAATTGAAGTCACATGGCCA+CGG + chr8_2:66038080-66038099 Msa1263010:intron 45.0%
GTAGTCACTGAGAGAGAATG+GGG - chr8_2:66038218-66038237 None:intergenic 45.0%
GTTAGGAAGAGAGAGAATGG+TGG - chr8_2:66038247-66038266 None:intergenic 45.0%
GATAGATGCAGAACAAGGGA+AGG + chr8_2:66038880-66038899 Msa1263010:CDS 45.0%
AAGGGAAGGTCACAGTTTCA+GGG + chr8_2:66038894-66038913 Msa1263010:CDS 45.0%
CAGAATCAAAACCAGAAGGG+TGG + chr8_2:66039088-66039107 Msa1263010:CDS 45.0%
AATCAAAACCAGAAGGGTGG+TGG + chr8_2:66039091-66039110 Msa1263010:CDS 45.0%
TATTGTTTCCACCACCCTTC+TGG - chr8_2:66039102-66039121 None:intergenic 45.0%
TTTGTTGACCACCTTTGGGT+TGG - chr8_2:66039126-66039145 None:intergenic 45.0%
!! TAGGATTCTGATTCGGGTTC+TGG - chr8_2:66039294-66039313 None:intergenic 45.0%
GGGGGTTTCATCTTATTAGG+TGG - chr8_2:66039454-66039473 None:intergenic 45.0%
AAACCCCCTTCTATGCCTAT+GGG + chr8_2:66039466-66039485 Msa1263010:CDS 45.0%
ATTCCCCATAGGCATAGAAG+GGG - chr8_2:66039473-66039492 None:intergenic 45.0%
CATTCCCCATAGGCATAGAA+GGG - chr8_2:66039474-66039493 None:intergenic 45.0%
GGTGCTCACATGATGTTGAA+TGG + chr8_2:66039493-66039512 Msa1263010:CDS 45.0%
CTTATGAGTGCTATGAAGGG+TGG + chr8_2:66039529-66039548 Msa1263010:CDS 45.0%
!! GGTGGCAATTTTAATGGTGG+TGG + chr8_2:66039547-66039566 Msa1263010:CDS 45.0%
!!! GGCAATTTTAATGGTGGTGG+TGG + chr8_2:66039550-66039569 Msa1263010:CDS 45.0%
! CATGGCATGAATGGTGGAAA+TGG + chr8_2:66039622-66039641 Msa1263010:CDS 45.0%
! AATGGTGGAAATGGCAATGG+AGG + chr8_2:66039631-66039650 Msa1263010:CDS 45.0%
AATGGAGGAAAGAAAGGTGG+TGG + chr8_2:66039646-66039665 Msa1263010:CDS 45.0%
GGGGGGAATAATCAGAATCA+AGG + chr8_2:66039682-66039701 Msa1263010:CDS 45.0%
! GTGGTGGAAACAAGAATGGA+GGG + chr8_2:66039752-66039771 Msa1263010:CDS 45.0%
GGTATGTCGATACCAATTGG+TGG + chr8_2:66039814-66039833 Msa1263010:CDS 45.0%
ATGTCGATACCAATTGGTGG+CGG + chr8_2:66039817-66039836 Msa1263010:CDS 45.0%
!! CAAAAAATGATGGGTGGTGG+AGG + chr8_2:66039886-66039905 Msa1263010:CDS 45.0%
!! GGGTGGTGGAGGAAATAATA+TGG + chr8_2:66039936-66039955 Msa1263010:CDS 45.0%
CGGTTCATAGGCATACTCAT+TGG - chr8_2:66039964-66039983 None:intergenic 45.0%
GAGTATGCCTATGAACCGAA+TGG + chr8_2:66039966-66039985 Msa1263010:CDS 45.0%
TTACCACCCATTCGGTTCAT+AGG - chr8_2:66039976-66039995 None:intergenic 45.0%
CAGGAATGTTACCACCCATT+CGG - chr8_2:66039984-66040003 None:intergenic 45.0%
GGTAACATTCCTGCTGTTCA+AGG + chr8_2:66039991-66040010 Msa1263010:CDS 45.0%
TGCTGCTGCTGTTGTTGATA+TGG - chr8_2:66040090-66040109 None:intergenic 45.0%
! TCATTGGCTGCTGAAATCTC+TGG - chr8_2:66040182-66040201 None:intergenic 45.0%
AGCAGCCAATGATGTATGCA+AGG + chr8_2:66040190-66040209 Msa1263010:CDS 45.0%
AAGGATCTGGAGGAGGATAA+GGG - chr8_2:66040251-66040270 None:intergenic 45.0%
TAAGGATCTGGAGGAGGATA+AGG - chr8_2:66040252-66040271 None:intergenic 45.0%
! TCGCTGAAAGCATGTGAGTA+AGG - chr8_2:66040270-66040289 None:intergenic 45.0%
GAATCACATGACATTGCAGC+TGG - chr8_2:66040304-66040323 None:intergenic 45.0%
! TGACCCCCTTTTCATCTTAG+GGG + chr8_2:66040473-66040492 Msa1263010:three_prime_UTR 45.0%
AACCCCCTAAGATGAAAAGG+GGG - chr8_2:66040479-66040498 None:intergenic 45.0%
AGAAATGAGCAACCAGAGCT+GGG - chr8_2:66040688-66040707 None:intergenic 45.0%
!!! AATTTAATTTATTTTTTTGA+AGG + chr8_2:66037293-66037312 Msa1263010:intron 5.0%
CTCAAGCCTCAAGGCACAAA+TGG + chr8_2:66036807-66036826 Msa1263010:CDS 50.0%
AAGGTATGGGGTTCGAACCT+CGG + chr8_2:66037533-66037552 Msa1263010:intron 50.0%
CAATGATGCAAGAGTGGAGG+TGG - chr8_2:66037939-66037958 None:intergenic 50.0%
CGTGGCCATGTGACTTCAAT+TGG - chr8_2:66038082-66038101 None:intergenic 50.0%
CAAGGGAAGGTCACAGTTTC+AGG + chr8_2:66038893-66038912 Msa1263010:CDS 50.0%
GGTCACAGTTTCAGGGAATG+TGG + chr8_2:66038901-66038920 Msa1263010:CDS 50.0%
CAGGGAAACATGCCGAGTTA+TGG + chr8_2:66038957-66038976 Msa1263010:CDS 50.0%
GAAACCCCCTTCTATGCCTA+TGG + chr8_2:66039465-66039484 Msa1263010:CDS 50.0%
AACCCCCTTCTATGCCTATG+GGG + chr8_2:66039467-66039486 Msa1263010:CDS 50.0%
TTCCCCATAGGCATAGAAGG+GGG - chr8_2:66039472-66039491 None:intergenic 50.0%
CCATTCCCCATAGGCATAGA+AGG - chr8_2:66039475-66039494 None:intergenic 50.0%
CCTTCTATGCCTATGGGGAA+TGG + chr8_2:66039472-66039491 Msa1263010:CDS 50.0%
ATGTGAGCACCATTCCCCAT+AGG - chr8_2:66039484-66039503 None:intergenic 50.0%
GGAAATGGACAGAAACCTGG+TGG + chr8_2:66039574-66039593 Msa1263010:CDS 50.0%
AATGGACAGAAACCTGGTGG+TGG + chr8_2:66039577-66039596 Msa1263010:CDS 50.0%
CCATGAATTTGAACCGGCAC+AGG - chr8_2:66039607-66039626 None:intergenic 50.0%
!! CCTGTGCCGGTTCAAATTCA+TGG + chr8_2:66039604-66039623 Msa1263010:CDS 50.0%
GGCAATGGAGGAAAGAAAGG+TGG + chr8_2:66039643-66039662 Msa1263010:CDS 50.0%
!! GGTGGTGGAAACAAGAATGG+AGG + chr8_2:66039751-66039770 Msa1263010:CDS 50.0%
!! AAAATGATGGGTGGTGGAGG+TGG + chr8_2:66039889-66039908 Msa1263010:CDS 50.0%
TCTCATCCTGGTAGTATGGG+TGG + chr8_2:66039919-66039938 Msa1263010:CDS 50.0%
ATGCCTATGAACCGAATGGG+TGG + chr8_2:66039970-66039989 Msa1263010:CDS 50.0%
TCCTGCTGTTCAAGGTCTAC+CGG + chr8_2:66039999-66040018 Msa1263010:CDS 50.0%
! GGAGGAGGTTACTTTCAAGG+TGG + chr8_2:66040027-66040046 Msa1263010:CDS 50.0%
! GGAGGTTACTTTCAAGGTGG+TGG + chr8_2:66040030-66040049 Msa1263010:CDS 50.0%
! GGTTACTTTCAAGGTGGTGG+TGG + chr8_2:66040033-66040052 Msa1263010:CDS 50.0%
!! TACTTTCAAGGTGGTGGTGG+TGG + chr8_2:66040036-66040055 Msa1263010:CDS 50.0%
GGATTCCCCTGCATCATTTC+CGG - chr8_2:66040069-66040088 None:intergenic 50.0%
! GAACCAACAACGTGCTGCTA+TGG + chr8_2:66040146-66040165 Msa1263010:CDS 50.0%
! GGTGGCCTTGCATACATCAT+TGG - chr8_2:66040198-66040217 None:intergenic 50.0%
TCTGGAGGAGGATAAGGGTA+AGG - chr8_2:66040246-66040265 None:intergenic 50.0%
TGTGAGTAAGGATCTGGAGG+AGG - chr8_2:66040258-66040277 None:intergenic 50.0%
GCATGTGAGTAAGGATCTGG+AGG - chr8_2:66040261-66040280 None:intergenic 50.0%
! GACCCCCTTTTCATCTTAGG+GGG + chr8_2:66040474-66040493 Msa1263010:three_prime_UTR 50.0%
GAGAAATGAGCAACCAGAGC+TGG - chr8_2:66040689-66040708 None:intergenic 50.0%
! TTCTGGCCCGGATGCTGATT+CGG - chr8_2:66039277-66039296 None:intergenic 55.0%
!! TTCTGATTCGGGTTCTGGCC+CGG - chr8_2:66039289-66039308 None:intergenic 55.0%
!! AATGGTGGTGGTGGCGGAAA+TGG + chr8_2:66039559-66039578 Msa1263010:CDS 55.0%
GGCGGAAATGGACAGAAACC+TGG + chr8_2:66039571-66039590 Msa1263010:CDS 55.0%
! GGAGGAAAGAAAGGTGGTGG+TGG + chr8_2:66039649-66039668 Msa1263010:CDS 55.0%
!! GGAAAGAAAGGTGGTGGTGG+TGG + chr8_2:66039652-66039671 Msa1263010:CDS 55.0%
!! AAGAAAGGTGGTGGTGGTGG+TGG + chr8_2:66039655-66039674 Msa1263010:CDS 55.0%
!! TCGATACCAATTGGTGGCGG+AGG + chr8_2:66039820-66039839 Msa1263010:CDS 55.0%
!! ATACCAATTGGTGGCGGAGG+TGG + chr8_2:66039823-66039842 Msa1263010:CDS 55.0%
! AGGCGGTGGTGGTAATGTTC+AGG + chr8_2:66039846-66039865 Msa1263010:CDS 55.0%
! GGTGGTGGTCAATCTCATCC+TGG + chr8_2:66039907-66039926 Msa1263010:CDS 55.0%
CATCCTGGTAGTATGGGTGG+TGG + chr8_2:66039922-66039941 Msa1263010:CDS 55.0%
! GGCGGAGGAGGTTACTTTCA+AGG + chr8_2:66040024-66040043 Msa1263010:CDS 55.0%
TCCAGGACCGGAAATGATGC+AGG + chr8_2:66040059-66040078 Msa1263010:CDS 55.0%
CCAGGACCGGAAATGATGCA+GGG + chr8_2:66040060-66040079 Msa1263010:CDS 55.0%
CAGGACCGGAAATGATGCAG+GGG + chr8_2:66040061-66040080 Msa1263010:CDS 55.0%
CCACCATAGCAGCACGTTGT+TGG - chr8_2:66040152-66040171 None:intergenic 55.0%
! CCAACAACGTGCTGCTATGG+TGG + chr8_2:66040149-66040168 Msa1263010:CDS 55.0%
GGAGGAGGATAAGGGTAAGG+AGG - chr8_2:66040243-66040262 None:intergenic 55.0%
CTTGAGGCTTGAGCGATGCC+AGG - chr8_2:66036800-66036819 None:intergenic 60.0%
CTGGCATCGCTCAAGCCTCA+AGG + chr8_2:66036798-66036817 Msa1263010:CDS 60.0%
AGTTGCTGCTGCTGCTGCTG+AGG - chr8_2:66039172-66039191 None:intergenic 60.0%
GATGCCGCCGAATCAGCATC+CGG + chr8_2:66039267-66039286 Msa1263010:CDS 60.0%
ATGCCGCCGAATCAGCATCC+GGG + chr8_2:66039268-66039287 Msa1263010:CDS 60.0%
!! GGACAGAAACCTGGTGGTGG+AGG + chr8_2:66039580-66039599 Msa1263010:CDS 60.0%
!! AAAGGTGGTGGTGGTGGTGG+TGG + chr8_2:66039658-66039677 Msa1263010:CDS 60.0%
!! ATGATGGGTGGTGGAGGTGG+TGG + chr8_2:66039892-66039911 Msa1263010:CDS 60.0%
CCTCCACCACCCATACTACC+AGG - chr8_2:66039928-66039947 None:intergenic 60.0%
! CCTGGTAGTATGGGTGGTGG+AGG + chr8_2:66039925-66039944 Msa1263010:CDS 60.0%
GCCGGTAGACCTTGAACAGC+AGG - chr8_2:66040003-66040022 None:intergenic 60.0%
GCTGTTCAAGGTCTACCGGC+AGG + chr8_2:66040003-66040022 Msa1263010:CDS 60.0%
GTTCAAGGTCTACCGGCAGG+CGG + chr8_2:66040006-66040025 Msa1263010:CDS 60.0%
AAGTAACCTCCTCCGCCTGC+CGG - chr8_2:66040021-66040040 None:intergenic 60.0%
CCCTGCATCATTTCCGGTCC+TGG - chr8_2:66040063-66040082 None:intergenic 60.0%
GTATGCAAGGCCACCTCCTG+AGG + chr8_2:66040203-66040222 Msa1263010:CDS 60.0%
!! TGGCCCGGATGCTGATTCGG+CGG - chr8_2:66039274-66039293 None:intergenic 65.0%
!! TGGTGGTGGAGGACCTGTGC+CGG + chr8_2:66039591-66039610 Msa1263010:CDS 65.0%
!! GTGGTGGTGGTGGTGGTGGT+GGG + chr8_2:66039662-66039681 Msa1263010:CDS 65.0%
!! TGGTGGTGGTGGTGGTGGTG+GGG + chr8_2:66039663-66039682 Msa1263010:CDS 65.0%
CCTCCACCTCCGCCACCAAT+TGG - chr8_2:66039829-66039848 None:intergenic 65.0%
!! CCAATTGGTGGCGGAGGTGG+AGG + chr8_2:66039826-66039845 Msa1263010:CDS 65.0%
!! ATTGGTGGCGGAGGTGGAGG+CGG + chr8_2:66039829-66039848 Msa1263010:CDS 65.0%
!! CAAGGTGGTGGTGGTGGTCC+AGG + chr8_2:66040042-66040061 Msa1263010:CDS 65.0%
!! TGGTGGTGGTGGTCCAGGAC+CGG + chr8_2:66040047-66040066 Msa1263010:CDS 65.0%
GGCACAGGTCCTCCACCACC+AGG - chr8_2:66039592-66039611 None:intergenic 70.0%
!! GGTGGTGGTGGTGGTGGTGG+TGG + chr8_2:66039661-66039680 Msa1263010:CDS 70.0%
!! GGTGGTGGTGGTGGTGGTGG+GGG + chr8_2:66039661-66039680 Msa1263010:CDS 70.0%
!! GTGGTGGTGGTGGTGGTGGG+GGG + chr8_2:66039665-66039684 Msa1263010:CDS 70.0%
CAAGGTCTACCGGCAGGCGG+AGG + chr8_2:66040009-66040028 Msa1263010:CDS 70.0%
GGTCTACCGGCAGGCGGAGG+AGG + chr8_2:66040012-66040031 Msa1263010:CDS 75.0%
!!! GGTGGCGGAGGTGGAGGCGG+TGG + chr8_2:66039832-66039851 Msa1263010:CDS 80.0%
! GGCGGAGGTGGAGGCGGTGG+TGG + chr8_2:66039835-66039854 Msa1263010:CDS 80.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 66036734 66040742 66036734 ID=Msa1263010;Name=Msa1263010
chr8_2 mRNA 66036734 66040742 66036734 ID=Msa1263010-mRNA-1;Parent=Msa1263010;Name=Msa1263010-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0|0.5|0.66|0.66|1|1|3|422|545
chr8_2 exon 66036734 66036838 66036734 ID=Msa1263010-mRNA-1:exon:21523;Parent=Msa1263010-mRNA-1
chr8_2 exon 66038644 66038719 66038644 ID=Msa1263010-mRNA-1:exon:21524;Parent=Msa1263010-mRNA-1
chr8_2 exon 66038864 66040742 66038864 ID=Msa1263010-mRNA-1:exon:21525;Parent=Msa1263010-mRNA-1
chr8_2 CDS 66036734 66036838 66036734 ID=Msa1263010-mRNA-1:cds;Parent=Msa1263010-mRNA-1
chr8_2 CDS 66038644 66038719 66038644 ID=Msa1263010-mRNA-1:cds;Parent=Msa1263010-mRNA-1
chr8_2 CDS 66038864 66040320 66038864 ID=Msa1263010-mRNA-1:cds;Parent=Msa1263010-mRNA-1
chr8_2 three_prime_UTR 66040321 66040742 66040321 ID=Msa1263010-mRNA-1:three_prime_utr;Parent=Msa1263010-mRNA-1
Gene Sequence

>Msa1263010

ATGCCAGAAAAGAAGGATCAGAAAAATGAGAAAGTTTCTGGGTTGATAGATGCAGCTAAATTTCCTGGCATCGCTCAAGCCTCAAGGCACAAATGGAAAAGTAAAAAATCTGTTCTAAAAGTGAACATACACTGTGATGGATGTAAACACAAAGTAAAGAAAATCTTACAGAAAATTGATGGAGTATTTACAACTGAGATAGATGCAGAACAAGGGAAGGTCACAGTTTCAGGGAATGTGGACCCAAATGTTCTCATCAAGAAGCTTGCAAAATCAGGGAAACATGCCGAGTTATGGAGTGTTCCCAAACCAAACAATAATCAAAATAATAACCAGAACAACGTTGTTAATCAGTTAAAGAACATGCAAATTGACAATGTCAAAGGTGGTGGAAACAACAAAGGTCAGAATCAAAACCAGAAGGGTGGTGGAAACAATAACCAACCCAAAGGTGGTCAACAAATACAACAACTACAAGGACTAAATCCTCAGCAGCAGCAGCAGCAACTTCAGCTTCAACAACAGTTGCAACAACTTCAACAGATGAAAGGGTTTCAAGATCTAGCTCAATTTAAAGGCATGAAGATGCCGCCGAATCAGCATCCGGGCCAGAACCCGAATCAGAATCCTAAAGATGCTAAAGGTGTTAAATTTGATGTGCCTGAAGATGAGGATGATTTTAGTGATGATGAAATGGATGATTATGATGACGACGAAGATGATGATGAAGATTTTGATGATGAGTTTGATGATGAAATGGTTGGTTTGCCACCTAATAAGATGAAACCCCCTTCTATGCCTATGGGGAATGGTGCTCACATGATGTTGAATGGGAATCATCCACAGCTTATGAGTGCTATGAAGGGTGGCAATTTTAATGGTGGTGGTGGCGGAAATGGACAGAAACCTGGTGGTGGAGGACCTGTGCCGGTTCAAATTCATGGCATGAATGGTGGAAATGGCAATGGAGGAAAGAAAGGTGGTGGTGGTGGTGGTGGTGGGGGGAATAATCAGAATCAAGGTGGAGGTAACAAAAATAATGGTGGCATGCCAGAAGCTAAAAATAATGGTGGTGGAAACAAGAATGGAGGGAATAATGGTAGTAATCAAAATAACGATGGGGGTAAGAAGGGTATGTCGATACCAATTGGTGGCGGAGGTGGAGGCGGTGGTGGTAATGTTCAGGCTATGAATAATGGATTTCAAAAAATGATGGGTGGTGGAGGTGGTGGTCAATCTCATCCTGGTAGTATGGGTGGTGGAGGAAATAATATGGGGCCAATGAGTATGCCTATGAACCGAATGGGTGGTAACATTCCTGCTGTTCAAGGTCTACCGGCAGGCGGAGGAGGTTACTTTCAAGGTGGTGGTGGTGGTCCAGGACCGGAAATGATGCAGGGGAATCCATATCAACAACAGCAGCAGCAACAACAACAGCAACAGTTAATGGCTGCTGCAATGATGAACCAACAACGTGCTGCTATGGTGGAAAATGACCAGAGATTTCAGCAGCCAATGATGTATGCAAGGCCACCTCCTGAGGTGAATTATATGTATCCTCCTTACCCTTATCCTCCTCCAGATCCTTACTCACATGCTTTCAGCGATGAGAACACTTCCAGCTGCAATGTCATGTGA
Protein sequence

>Msa1263010

MPEKKDQKNEKVSGLIDAAKFPGIAQASRHKWKSKKSVLKVNIHCDGCKHKVKKILQKIDGVFTTEIDAEQGKVTVSGNVDPNVLIKKLAKSGKHAELWSVPKPNNNQNNNQNNVVNQLKNMQIDNVKGGGNNKGQNQNQKGGGNNNQPKGGQQIQQLQGLNPQQQQQQLQLQQQLQQLQQMKGFQDLAQFKGMKMPPNQHPGQNPNQNPKDAKGVKFDVPEDEDDFSDDEMDDYDDDEDDDEDFDDEFDDEMVGLPPNKMKPPSMPMGNGAHMMLNGNHPQLMSAMKGGNFNGGGGGNGQKPGGGGPVPVQIHGMNGGNGNGGKKGGGGGGGGGNNQNQGGGNKNNGGMPEAKNNGGGNKNGGNNGSNQNNDGGKKGMSIPIGGGGGGGGGNVQAMNNGFQKMMGGGGGGQSHPGSMGGGGNNMGPMSMPMNRMGGNIPAVQGLPAGGGGYFQGGGGGPGPEMMQGNPYQQQQQQQQQQQLMAAAMMNQQRAAMVENDQRFQQPMMYARPPPEVNYMYPPYPYPPPDPYSHAFSDENTSSCNVM*