AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0315980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0315980 MtrunA17_Chr2g0287751 88.538 506 40 7 1 500 1 494 3.43e-173 496
Msa0315980 MtrunA17_Chr2g0287741 72.379 496 105 8 10 500 15 483 5.29e-129 386
Msa0315980 MtrunA17_Chr4g0065651 51.812 552 174 21 1 500 1 512 2.64e-54 190
Msa0315980 MtrunA17_Chr4g0065621 70.466 193 44 7 1 188 1 185 7.36e-46 164
Msa0315980 MtrunA17_Chr0c22g0493881 71.354 192 44 6 1 188 1 185 1.89e-45 163
Msa0315980 MtrunA17_Chr1g0191671 49.746 197 57 5 1 195 1 157 1.71e-36 142
Msa0315980 MtrunA17_Chr1g0208301 70.513 78 20 1 1 75 1 78 2.50e-30 121
Msa0315980 MtrunA17_Chr7g0269081 67.143 70 23 0 5 74 17 86 8.95e-25 104
Msa0315980 MtrunA17_Chr4g0067291 52.525 99 44 2 7 102 71 169 2.27e-23 101
Msa0315980 MtrunA17_Chr1g0192131 58.667 75 30 1 4 77 9 83 1.56e-19 90.5
Msa0315980 MtrunA17_Chr3g0111541 53.521 71 33 0 3 73 5 75 1.89e-19 87.0
Msa0315980 MtrunA17_Chr7g0272571 56.061 66 29 0 9 74 32 97 7.37e-19 88.2
Msa0315980 MtrunA17_Chr2g0296001 58.571 70 29 0 5 74 68 137 1.10e-16 81.6
Msa0315980 MtrunA17_Chr8g0382721 52.459 61 29 0 14 74 33 93 2.33e-14 70.5
Msa0315980 MtrunA17_Chr3g0124411 48.485 66 34 0 9 74 29 94 2.54e-13 67.8
Msa0315980 MtrunA17_Chr1g0160201 43.939 66 37 0 9 74 24 89 1.43e-12 65.5
Msa0315980 MtrunA17_Chr1g0198341 43.284 67 38 0 10 76 4 70 1.50e-12 63.2
Msa0315980 MtrunA17_Chr0c22g0493871 53.623 138 35 6 380 500 5 130 1.55e-12 64.7
Msa0315980 MtrunA17_Chr4g0065611 53.623 138 35 6 380 500 17 142 1.77e-12 65.1
Msa0315980 MtrunA17_Chr5g0407821 40.278 72 43 0 9 80 22 93 8.08e-12 63.2
Msa0315980 MtrunA17_Chr1g0189221 38.028 71 44 0 7 77 50 120 1.05e-11 63.5
Msa0315980 MtrunA17_Chr7g0218981 42.647 68 39 0 13 80 7 74 1.06e-11 62.4
Msa0315980 MtrunA17_Chr2g0325601 38.750 80 47 1 14 91 6 85 2.70e-11 61.6
Msa0315980 MtrunA17_Chr5g0404641 41.538 65 38 0 9 73 2 66 6.28e-11 60.8
Msa0315980 MtrunA17_Chr7g0251491 43.548 62 34 1 14 74 52 113 6.54e-11 61.2
Msa0315980 MtrunA17_Chr4g0036931 36.986 73 45 1 3 74 21 93 8.95e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0315980 AT3G06130.2 55.769 260 97 7 1 248 1 254 3.13e-47 167
Msa0315980 AT3G06130.1 55.769 260 97 7 1 248 1 254 4.08e-46 167
Msa0315980 AT5G19090.2 74.380 121 24 2 1 114 1 121 2.36e-45 165
Msa0315980 AT5G19090.2 47.222 144 41 5 389 500 325 465 4.73e-13 71.6
Msa0315980 AT5G19090.3 74.380 121 24 2 1 114 1 121 2.36e-45 165
Msa0315980 AT5G19090.3 47.222 144 41 5 389 500 325 465 4.73e-13 71.6
Msa0315980 AT5G19090.5 74.380 121 24 2 1 114 1 121 1.29e-44 166
Msa0315980 AT5G19090.4 74.380 121 24 2 1 114 1 121 1.29e-44 166
Msa0315980 AT5G19090.1 74.380 121 24 2 1 114 1 121 1.29e-44 166
Msa0315980 AT3G05220.1 62.162 74 28 0 1 74 1 74 4.89e-26 112
Msa0315980 AT5G27690.1 49.074 108 54 1 5 112 25 131 1.89e-24 104
Msa0315980 AT1G23000.1 60.256 78 28 1 1 75 1 78 3.19e-24 104
Msa0315980 AT1G23000.2 60.256 78 28 1 1 75 61 138 8.53e-24 103
Msa0315980 AT5G37860.1 58.462 65 27 0 9 73 9 73 1.05e-19 89.4
Msa0315980 AT1G56210.1 60.317 63 25 0 10 72 40 102 1.31e-17 84.7
Msa0315980 AT1G06330.1 48.438 64 33 0 14 77 6 69 6.37e-14 69.7
Msa0315980 AT1G66240.1 40.299 67 40 0 10 76 33 99 3.76e-12 63.2
Msa0315980 AT3G56891.1 42.188 64 37 0 14 77 20 83 5.53e-11 61.6
Msa0315980 AT1G71050.1 39.394 66 40 0 9 74 27 92 7.95e-11 60.8

Find 195 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGGGAAGGTGACCGTTTC+TGG 0.126298 2_4:+12727962 Msa0315980:CDS
AAGAATTTCACGATTAAAAT+AGG 0.136483 2_4:+12726527 Msa0315980:five_prime_UTR
AGCTTCTTGATGAGAATATT+TGG 0.155111 2_4:-12727995 None:intergenic
CTTCAAAATATTAAAGGATT+TGG 0.214990 2_4:+12728274 Msa0315980:CDS
GAACAACTTCAAAATATTAA+AGG 0.221872 2_4:+12728268 Msa0315980:CDS
TTTCCACCCATCATCATTTC+AGG 0.251317 2_4:-12729081 None:intergenic
GGTGGAGGTGGTGGTAATGG+TGG 0.278078 2_4:+12728778 Msa0315980:CDS
ATCAAGAAGCTTGCAAAATC+AGG 0.297726 2_4:+12728007 Msa0315980:CDS
CCTGTGCCTGTTCAAGTTCA+TGG 0.322029 2_4:+12728598 Msa0315980:CDS
GATGAGAAATGTGAGGCATT+AGG 0.324090 2_4:-12726728 None:intergenic
CATGGCTTGGGTGGTGGAAA+TGG 0.328082 2_4:+12728616 Msa0315980:CDS
ATGAGAAATGTGAGGCATTA+GGG 0.329003 2_4:-12726727 None:intergenic
AGGGAAACATGCAGAACTTT+GGG 0.332545 2_4:+12728027 Msa0315980:CDS
GGAGGTGGAGGTGGTGGTAA+TGG 0.333065 2_4:+12728775 Msa0315980:CDS
TCTATAGTAATTTAAAGTTC+AGG 0.341064 2_4:-12729464 None:intergenic
TTATTGTTGTTGTTTGCCTT+TGG 0.363526 2_4:-12728055 None:intergenic
CCCATTGCTCCTCCTCCCAT+TGG 0.369706 2_4:-12728943 None:intergenic
TTTGATTTGCAACGTTGTTC+TGG 0.372533 2_4:-12728081 None:intergenic
CAGCCATGTATTGTTGTTGT+TGG 0.375921 2_4:-12729110 None:intergenic
AAAGGTGGTGGCGGTGGCGG+TGG 0.381550 2_4:+12728652 Msa0315980:CDS
GGATGACCACCACCCATGTT+TGG 0.385549 2_4:-12728880 None:intergenic
GCCTGTTCAAGTTCATGGCT+TGG 0.385983 2_4:+12728603 Msa0315980:CDS
GCTTCTTGATGAGAATATTT+GGG 0.388512 2_4:-12727994 None:intergenic
AATAAGAATGTTGGTGGAGG+TGG 0.390096 2_4:+12728760 Msa0315980:CDS
AAAATCTTGCAGAAGATTGA+TGG 0.391119 2_4:+12727295 Msa0315980:CDS
ATGTATTGTTGTTGTTGGTA+TGG 0.391454 2_4:-12729105 None:intergenic
GGCGGTGTCAGTGGTGGATA+TGG 0.391475 2_4:+12729054 Msa0315980:CDS
AGAATTTCACGATTAAAATA+GGG 0.391505 2_4:+12726528 Msa0315980:five_prime_UTR
GCCGGTAGGCCTTGAACAGC+TGG 0.391650 2_4:-12729006 None:intergenic
CCCATAAATAATGGCACAAA+AGG 0.402278 2_4:+12726605 Msa0315980:five_prime_UTR
CAGGGAAACATGCAGAACTT+TGG 0.405425 2_4:+12728026 Msa0315980:CDS
AAGAAAGGTGGTGGCGGTGG+CGG 0.406323 2_4:+12728649 Msa0315980:CDS
GTTCAAGGTATGATGAATAA+TGG 0.407885 2_4:+12728853 Msa0315980:CDS
GGTGGTGGCGGCAGTGGTGG+AGG 0.409161 2_4:+12728574 Msa0315980:CDS
AAGAAAGGTGGTGGCGGCAG+TGG 0.415819 2_4:+12728568 Msa0315980:CDS
GTTGGTGGAGGTGGAGGTGG+TGG 0.416438 2_4:+12728769 Msa0315980:CDS
GAAACCATGTGGCCCACATT+AGG 0.418420 2_4:-12728901 None:intergenic
CCTCCCATTTGACTCATCAT+TGG 0.422725 2_4:-12728976 None:intergenic
CATGATGATGAATGCTCAAA+AGG 0.426250 2_4:+12728519 Msa0315980:CDS
AATGTTGGTGGAGGTGGAGG+TGG 0.427853 2_4:+12728766 Msa0315980:CDS
CCCAAGCCATGAACTTGAAC+AGG 0.428896 2_4:-12728604 None:intergenic
TTTAGCAACCATGGATGTCT+TGG 0.429098 2_4:-12726574 None:intergenic
TGTATTGTTGTTGTTGGTAT+GGG 0.429195 2_4:-12729104 None:intergenic
AAAGGTGGTGGCGGCAGTGG+TGG 0.433138 2_4:+12728571 Msa0315980:CDS
TCTGAAGGTGCCTCAATTCA+AGG 0.437147 2_4:+12728300 Msa0315980:CDS
CCTGTTCAAGTTCATGGCTT+GGG 0.438192 2_4:+12728604 Msa0315980:CDS
CTCATTTCTTTCAAAACTCA+AGG 0.440002 2_4:+12729614 Msa0315980:three_prime_UTR
ATGGCTTGGGTGGTGGAAAT+GGG 0.442661 2_4:+12728617 Msa0315980:CDS
GAATAAGATGAAACTACCTA+TGG 0.442921 2_4:+12728477 Msa0315980:CDS
GGAAAGAAAGGTGGTGGCGG+TGG 0.448783 2_4:+12728646 Msa0315980:CDS
CAAAAGGGTGGTGGCCAACA+AGG 0.452414 2_4:+12728193 Msa0315980:CDS
AACAAGGGGCAAAAGGGTCC+TGG 0.452988 2_4:+12728154 Msa0315980:CDS
ATGGGAGAAAGTGGTGTTCA+AGG 0.452988 2_4:+12728838 Msa0315980:CDS
GAAGAAATGAGATGATGAAT+TGG 0.455801 2_4:+12729328 Msa0315980:exon
GCTCAAAAGGGTGTTGGAAA+TGG 0.456364 2_4:+12728532 Msa0315980:CDS
GGTGGAGGAGGGTAGCTATA+CGG 0.456831 2_4:-12729237 None:intergenic
AAATAACCAAAATCAGCAAA+AGG 0.459158 2_4:+12728177 Msa0315980:CDS
ATCAAAGGGTTCTAACAAAG+AGG 0.460794 2_4:-12726964 None:intergenic
GTGGGATTCCAAATAACAAT+GGG 0.461498 2_4:+12728797 Msa0315980:CDS
AAGAATGTTGGTGGAGGTGG+AGG 0.463322 2_4:+12728763 Msa0315980:CDS
GGTGGTCTTGCGTAAATCAT+TGG 0.463489 2_4:-12729186 None:intergenic
CGTGCCAATGATGAGTCAAA+TGG 0.467699 2_4:+12728972 Msa0315980:CDS
TGAGGATGTTGAGGTGGAGG+AGG 0.468950 2_4:-12729249 None:intergenic
GCGATGAACGGCGGTGGAGG+AGG 0.468951 2_4:+12729033 Msa0315980:CDS
GTTGGAAATGGCGGAGGCAA+TGG 0.471383 2_4:+12728544 Msa0315980:CDS
ATGTTCATGTCCTTGAATTG+AGG 0.472918 2_4:-12728310 None:intergenic
ATGCAAATTGACAATGGAAA+AGG 0.473035 2_4:+12728112 Msa0315980:CDS
GCTGCGATGAACGGCGGTGG+AGG 0.477384 2_4:+12729030 Msa0315980:CDS
TTAGCAACCATGGATGTCTT+GGG 0.479505 2_4:-12726573 None:intergenic
GGGTGGTGGTCATCCTAATG+TGG 0.482164 2_4:+12728888 Msa0315980:CDS
GGAGGAGGCGGTGTCAGTGG+TGG 0.483447 2_4:+12729048 Msa0315980:CDS
GGTAACATGCCAGCTGTTCA+AGG 0.483877 2_4:+12728997 Msa0315980:CDS
GTGGAGGTGGTGGTAATGGT+GGG 0.486444 2_4:+12728779 Msa0315980:CDS
ATGAACGGCGGTGGAGGAGG+CGG 0.489060 2_4:+12729036 Msa0315980:CDS
TCAACAACAGTTACAACAGC+TGG 0.491026 2_4:+12728246 Msa0315980:CDS
CAAGTTCATGGCTTGGGTGG+TGG 0.495665 2_4:+12728610 Msa0315980:CDS
AGGGAGAAGATGAACAGAAA+GGG 0.496177 2_4:-12726708 None:intergenic
TGGGTCCACATTTCCAGAAA+CGG 0.497187 2_4:-12727975 None:intergenic
AATCTATCATTTCCACCGGC+TGG 0.497223 2_4:-12729159 None:intergenic
GCCAGCTGTTCAAGGCCTAC+CGG 0.501295 2_4:+12729005 Msa0315980:CDS
CCAATGGGAGGAGGAGCAAT+GGG 0.508430 2_4:+12728943 Msa0315980:CDS
GCAATGGGAGAAAAGAAAGA+AGG 0.508873 2_4:-12726838 None:intergenic
TCTTGAGGATGTTGAGGTGG+AGG 0.508997 2_4:-12729252 None:intergenic
GACAATGGAAAAGGTGGTGG+AGG 0.511844 2_4:+12728121 Msa0315980:CDS
GGGAGAAGATGAACAGAAAG+GGG 0.514669 2_4:-12726707 None:intergenic
GGTAGTTTCATCTTATTCAA+AGG 0.515445 2_4:-12728472 None:intergenic
GCAGCTGCGATGAACGGCGG+TGG 0.515631 2_4:+12729027 Msa0315980:CDS
AATGGGAATGGAGGAAAGAA+AGG 0.515830 2_4:+12728634 Msa0315980:CDS
CCGCCGTTCATCGCAGCTGC+CGG 0.518191 2_4:-12729024 None:intergenic
CAATATGAATGGTATGCCAA+TGG 0.523524 2_4:+12728927 Msa0315980:CDS
ATGGTTTCTAACAATATGAA+TGG 0.524254 2_4:+12728916 Msa0315980:CDS
GATTTACGCAAGACCACCAA+TGG 0.525992 2_4:+12729191 Msa0315980:CDS
CTACCGGCAGCTGCGATGAA+CGG 0.526265 2_4:+12729021 Msa0315980:CDS
GGCGGAGGCAATGGAAAGAA+AGG 0.528122 2_4:+12728553 Msa0315980:CDS
ATGAATGCTCAAAAGGGTGT+TGG 0.528773 2_4:+12728526 Msa0315980:CDS
GAGGATGTTGAGGTGGAGGA+GGG 0.529310 2_4:-12729248 None:intergenic
AGATGATGAATTGGTGATGC+TGG 0.533354 2_4:+12729337 Msa0315980:three_prime_UTR
GGAGGAAAGAAAGGTGGTGG+CGG 0.534225 2_4:+12728643 Msa0315980:CDS
CAATGGGAGAAAAGAAAGAA+GGG 0.534511 2_4:-12726837 None:intergenic
AAAGAGGGAGAAATGTAGAA+AGG 0.536287 2_4:-12726948 None:intergenic
GTAAATGGGATGGGAGAAAG+TGG 0.537153 2_4:+12728829 Msa0315980:CDS
CGTTCATCGCAGCTGCCGGT+AGG 0.538577 2_4:-12729020 None:intergenic
TTGAAATCTATCATTTCCAC+CGG 0.540400 2_4:-12729163 None:intergenic
GGTGGAGGAGGCGGTGTCAG+TGG 0.540462 2_4:+12729045 Msa0315980:CDS
GTGCCAATGATGAGTCAAAT+GGG 0.540610 2_4:+12728973 Msa0315980:CDS
GCCAATGGGAGGAGGAGCAA+TGG 0.540634 2_4:+12728942 Msa0315980:CDS
AATAAGATGAAACTACCTAT+GGG 0.540693 2_4:+12728478 Msa0315980:CDS
TGGTGGTCCTGAAATGATGA+TGG 0.543773 2_4:+12729074 Msa0315980:CDS
AGAACTTTGGGGTGCTCCAA+AGG 0.546283 2_4:+12728039 Msa0315980:CDS
TGCAATGATGAATCAACAAA+GGG 0.546917 2_4:+12729131 Msa0315980:CDS
TCATCCTAATGTGGGCCACA+TGG 0.547730 2_4:+12728897 Msa0315980:CDS
AATATGAATGGTATGCCAAT+GGG 0.548285 2_4:+12728928 Msa0315980:CDS
TACATGTAATTAACACCCAT+TGG 0.549609 2_4:-12729207 None:intergenic
GGGGTGTGAAGAAGAAGGCT+TGG 0.550165 2_4:-12726796 None:intergenic
GCATTCATCATCATGTGAGC+AGG 0.550437 2_4:-12728511 None:intergenic
TAGGGAGAAGATGAACAGAA+AGG 0.551050 2_4:-12726709 None:intergenic
TAAAGGATTTGGAGATCTGA+AGG 0.551549 2_4:+12728285 Msa0315980:CDS
AATGGGAATAAGAATGTTGG+TGG 0.551727 2_4:+12728754 Msa0315980:CDS
TCAAGAAGCTTGCAAAATCA+GGG 0.551852 2_4:+12728008 Msa0315980:CDS
CTCATCATCAAACTCCTCAT+CGG 0.556584 2_4:-12728440 None:intergenic
CAACAAAGGGCAATACCAGC+CGG 0.559250 2_4:+12729144 Msa0315980:CDS
TCAAAGGGTTCTAACAAAGA+GGG 0.561418 2_4:-12726963 None:intergenic
CAAAATCAGCAAAAGGGTGG+TGG 0.562661 2_4:+12728184 Msa0315980:CDS
ATTGACAATGGAAAAGGTGG+TGG 0.565333 2_4:+12728118 Msa0315980:CDS
TTGGGTGGTGGAAATGGGAA+TGG 0.566802 2_4:+12728622 Msa0315980:CDS
AATAACCAAAATCAGCAAAA+GGG 0.567885 2_4:+12728178 Msa0315980:CDS
AAGTTGGAGTATGGATCTTG+AGG 0.572375 2_4:-12729267 None:intergenic
AAAGTTTCCCAAGACATCCA+TGG 0.573799 2_4:+12726566 Msa0315980:five_prime_UTR
GGTGGTGGTCATCCTAATGT+GGG 0.574953 2_4:+12728889 Msa0315980:CDS
AATGGTATGCCAATGGGAGG+AGG 0.574960 2_4:+12728934 Msa0315980:CDS
GGCAATGGAAAGAAAGGTGG+TGG 0.576650 2_4:+12728559 Msa0315980:CDS
AATGGAGGAAAGAAAGGTGG+TGG 0.577240 2_4:+12728640 Msa0315980:CDS
AATAATCAGAGTCAGAATCA+AGG 0.578254 2_4:+12728676 Msa0315980:CDS
AGGGTGTGTGAATATAAGCA+AGG 0.579993 2_4:-12726818 None:intergenic
AAGAACATGCAAATTGACAA+TGG 0.586548 2_4:+12728106 Msa0315980:CDS
GGGTGTGTGAATATAAGCAA+GGG 0.587286 2_4:-12726817 None:intergenic
TAAGATGAAACTACCTATGG+GGG 0.588463 2_4:+12728480 Msa0315980:CDS
CTGCAATGATGAATCAACAA+AGG 0.588706 2_4:+12729130 Msa0315980:CDS
GGAGGGTAGCTATACGGCGG+AGG 0.592003 2_4:-12729231 None:intergenic
CCATGAACTTGAACAGGCAC+AGG 0.594078 2_4:-12728598 None:intergenic
AAGGGTGTTGGAAATGGCGG+AGG 0.594852 2_4:+12728538 Msa0315980:CDS
CAGAGTCAGAATCAAGGTGG+AGG 0.601904 2_4:+12728682 Msa0315980:CDS
GGTGACCGTTTCTGGAAATG+TGG 0.604667 2_4:+12727970 Msa0315980:CDS
GAAAGGTTGAGAACATAACT+AGG 0.604718 2_4:-12726931 None:intergenic
ATACCAACAACAACAATACA+TGG 0.604836 2_4:+12729107 Msa0315980:CDS
TATGGATCTTGAGGATGTTG+AGG 0.606500 2_4:-12729258 None:intergenic
GTTCAAGTTCATGGCTTGGG+TGG 0.606576 2_4:+12728607 Msa0315980:CDS
GGATCTTGAGGATGTTGAGG+TGG 0.607090 2_4:-12729255 None:intergenic
GGACTTCCAAACATGGGTGG+TGG 0.608332 2_4:+12728874 Msa0315980:CDS
ATTTACGCAAGACCACCAAT+GGG 0.609778 2_4:+12729192 Msa0315980:CDS
AGAGAAAGATGAGAAATGTG+AGG 0.610284 2_4:-12726735 None:intergenic
GAATAATGGACTTCCAAACA+TGG 0.610386 2_4:+12728867 Msa0315980:CDS
ACTGAGATAGATGCTGAACA+AGG 0.610845 2_4:+12727944 Msa0315980:CDS
GGTGGTGGAAATGGGAATGG+AGG 0.610911 2_4:+12728625 Msa0315980:CDS
TGAGGAGTTTGATGATGAGA+TGG 0.610932 2_4:+12728444 Msa0315980:CDS
ATGTAATTAACACCCATTGG+TGG 0.615835 2_4:-12729204 None:intergenic
GGTGTCAGTGGTGGATATGG+TGG 0.616024 2_4:+12729057 Msa0315980:CDS
ATGATGATGAATGCTCAAAA+GGG 0.619348 2_4:+12728520 Msa0315980:CDS
GGGTATGTATTTAGCAACCA+TGG 0.622251 2_4:-12726583 None:intergenic
GGGAATAAGAATGTTGGTGG+AGG 0.623326 2_4:+12728757 Msa0315980:CDS
AATCAAAATACAAACACGAA+AGG 0.624431 2_4:+12728349 Msa0315980:CDS
AATGGAAAGAAAGGTGGTGG+CGG 0.625102 2_4:+12728562 Msa0315980:CDS
GATAGATGCTGAACAAGGGA+AGG 0.625794 2_4:+12727949 Msa0315980:CDS
CAAAAGGGTGTTGGAAATGG+CGG 0.630177 2_4:+12728535 Msa0315980:CDS
CCGGCAGCTGCGATGAACGG+CGG 0.637137 2_4:+12729024 Msa0315980:CDS
GGGAATGGAGGAAAGAAAGG+TGG 0.637548 2_4:+12728637 Msa0315980:CDS
GGGAAACATGCAGAACTTTG+GGG 0.640585 2_4:+12728028 Msa0315980:CDS
ATAAGATGAAACTACCTATG+GGG 0.647445 2_4:+12728479 Msa0315980:CDS
CAAATTGACAATGGAAAAGG+TGG 0.648543 2_4:+12728115 Msa0315980:CDS
AAAAGGGTGGTGGCCAACAA+GGG 0.648685 2_4:+12728194 Msa0315980:CDS
AAATGGAGGTGGAATGCCGG+AGG 0.650010 2_4:+12728726 Msa0315980:CDS
CTGAGATAGATGCTGAACAA+GGG 0.650734 2_4:+12727945 Msa0315980:CDS
GGTGGGATTCCAAATAACAA+TGG 0.655401 2_4:+12728796 Msa0315980:CDS
AATAATGGACTTCCAAACAT+GGG 0.656303 2_4:+12728868 Msa0315980:CDS
AACCAAAATCAGCAAAAGGG+TGG 0.657132 2_4:+12728181 Msa0315980:CDS
GGTGGAGGTAATAAGAACAA+TGG 0.658879 2_4:+12728697 Msa0315980:CDS
CAAAGGGCAATACCAGCCGG+TGG 0.662288 2_4:+12729147 Msa0315980:CDS
AATGGAGGTGGAATGCCGGA+GGG 0.666899 2_4:+12728727 Msa0315980:CDS
ATGAATGGTATGCCAATGGG+AGG 0.670599 2_4:+12728931 Msa0315980:CDS
GGTGTGTGAATATAAGCAAG+GGG 0.670704 2_4:-12726816 None:intergenic
TCATATTGTTAGAAACCATG+TGG 0.673268 2_4:-12728912 None:intergenic
GGAGGTAATAAGAACAATGG+TGG 0.674022 2_4:+12728700 Msa0315980:CDS
GGGATTCCAAATAACAATGG+GGG 0.674410 2_4:+12728799 Msa0315980:CDS
AAAGTGAACATACACTGTGA+CGG 0.675992 2_4:+12727253 Msa0315980:CDS
GGTGGTCCTGAAATGATGAT+GGG 0.676791 2_4:+12729075 Msa0315980:CDS
GGTCCTGAAATGATGATGGG+TGG 0.683123 2_4:+12729078 Msa0315980:CDS
AATCAGAGTCAGAATCAAGG+TGG 0.685165 2_4:+12728679 Msa0315980:CDS
AATGGACTTCCAAACATGGG+TGG 0.695955 2_4:+12728871 Msa0315980:CDS
GGAGGCAATGGAAAGAAAGG+TGG 0.701098 2_4:+12728556 Msa0315980:CDS
AAGATGAAACTACCTATGGG+GGG 0.701851 2_4:+12728481 Msa0315980:CDS
GTGTGTGAATATAAGCAAGG+GGG 0.725063 2_4:-12726815 None:intergenic
TGGGATTCCAAATAACAATG+GGG 0.736086 2_4:+12728798 Msa0315980:CDS
CCAATGATGAGTCAAATGGG+AGG 0.737345 2_4:+12728976 Msa0315980:CDS
TGATGATGAGTATTCCGATG+AGG 0.755531 2_4:+12728426 Msa0315980:CDS
GGAGGAGGGTAGCTATACGG+CGG 0.757400 2_4:-12729234 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTAAAAGAAAAAAATG+GGG + chr2_4:12729556-12729575 Msa0315980:three_prime_UTR 10.0%
!!! ATATTTAGTTTTATAGATGA+AGG - chr2_4:12726872-12726891 None:intergenic 15.0%
!!! CTCATTTTTTTTTTATCAAA+GGG - chr2_4:12726981-12727000 None:intergenic 15.0%
!!! ACTCATTTTTTTTTTATCAA+AGG - chr2_4:12726982-12727001 None:intergenic 15.0%
!!! TATGAGTATTTTTTTTTCAT+GGG - chr2_4:12727625-12727644 None:intergenic 15.0%
!!! TTATGAGTATTTTTTTTTCA+TGG - chr2_4:12727626-12727645 None:intergenic 15.0%
!!! ATTGTGTAATTTAATTTTGT+AGG + chr2_4:12727911-12727930 Msa0315980:intron 15.0%
!!! TTGTGTAATTTAATTTTGTA+GGG + chr2_4:12727912-12727931 Msa0315980:intron 15.0%
!! AAAAAACTTAGATGAAAAAA+AGG - chr2_4:12729431-12729450 None:intergenic 15.0%
!! AATTTAAAAGAAAAAAATGG+GGG + chr2_4:12729557-12729576 Msa0315980:three_prime_UTR 15.0%
!! AAGAATTTCACGATTAAAAT+AGG + chr2_4:12726527-12726546 Msa0315980:five_prime_UTR 20.0%
!! AGAATTTCACGATTAAAATA+GGG + chr2_4:12726528-12726547 Msa0315980:five_prime_UTR 20.0%
!! ATGACATTTCTAATAAAGTA+AGG + chr2_4:12727150-12727169 Msa0315980:intron 20.0%
!!! AAACTTTTTTTTTAGCAAGT+TGG - chr2_4:12727346-12727365 None:intergenic 20.0%
!!! AGGTATATACATATTTTTCT+TGG - chr2_4:12727695-12727714 None:intergenic 20.0%
!!! ATCATTTAAGATATAACAAG+TGG - chr2_4:12727846-12727865 None:intergenic 20.0%
!!! TGTGTAATTTAATTTTGTAG+GGG + chr2_4:12727913-12727932 Msa0315980:intron 20.0%
!! GAACAACTTCAAAATATTAA+AGG + chr2_4:12728268-12728287 Msa0315980:CDS 20.0%
!! CTTCAAAATATTAAAGGATT+TGG + chr2_4:12728274-12728293 Msa0315980:CDS 20.0%
!!! TCTATAGTAATTTAAAGTTC+AGG - chr2_4:12729467-12729486 None:intergenic 20.0%
!!! AGTTTTATAGATGAAGGTTT+TGG - chr2_4:12726866-12726885 None:intergenic 25.0%
! GACTATGAATATGAATGAAA+AGG - chr2_4:12727376-12727395 None:intergenic 25.0%
!! ACTTTTCTTTGATCTGTTAT+TGG + chr2_4:12727561-12727580 Msa0315980:intron 25.0%
! GTATCAATATGAATGAAATG+AGG - chr2_4:12727715-12727734 None:intergenic 25.0%
!! CAAGAGTACAAAGTAAAATA+TGG - chr2_4:12727766-12727785 None:intergenic 25.0%
!! ATTTAAGATATAACAAGTGG+TGG - chr2_4:12727843-12727862 None:intergenic 25.0%
! AAATAACCAAAATCAGCAAA+AGG + chr2_4:12728177-12728196 Msa0315980:CDS 25.0%
! AATAACCAAAATCAGCAAAA+GGG + chr2_4:12728178-12728197 Msa0315980:CDS 25.0%
!!! TTCGTGTTTGTATTTTGATT+TGG - chr2_4:12728349-12728368 None:intergenic 25.0%
! AATCAAAATACAAACACGAA+AGG + chr2_4:12728349-12728368 Msa0315980:CDS 25.0%
! AATAAGATGAAACTACCTAT+GGG + chr2_4:12728478-12728497 Msa0315980:CDS 25.0%
! AAAAATGGGAATAAGAATGT+TGG + chr2_4:12728751-12728770 Msa0315980:CDS 25.0%
! ATGGTTTCTAACAATATGAA+TGG + chr2_4:12728916-12728935 Msa0315980:CDS 25.0%
ATACATACCCCCATAAATAA+TGG + chr2_4:12726596-12726615 Msa0315980:five_prime_UTR 30.0%
!! TTTTGTGCCATTATTTATGG+GGG - chr2_4:12726606-12726625 None:intergenic 30.0%
!! CTTTTGTGCCATTATTTATG+GGG - chr2_4:12726607-12726626 None:intergenic 30.0%
!! CCTTTTGTGCCATTATTTAT+GGG - chr2_4:12726608-12726627 None:intergenic 30.0%
!! TCCTTTTGTGCCATTATTTA+TGG - chr2_4:12726609-12726628 None:intergenic 30.0%
!! AGAAGAGATTTTGAAGATAC+AGG + chr2_4:12727004-12727023 Msa0315980:CDS 30.0%
! GTAACACTAATTCTTGCTTT+TGG + chr2_4:12727194-12727213 Msa0315980:intron 30.0%
!!! CTAATTCTTGCTTTTGGTTT+TGG + chr2_4:12727200-12727219 Msa0315980:intron 30.0%
AAAATCTTGCAGAAGATTGA+TGG + chr2_4:12727295-12727314 Msa0315980:CDS 30.0%
! ACATATTTTTCTTGGTCGAA+TGG - chr2_4:12727687-12727706 None:intergenic 30.0%
GCTTCTTGATGAGAATATTT+GGG - chr2_4:12727997-12728016 None:intergenic 30.0%
AGCTTCTTGATGAGAATATT+TGG - chr2_4:12727998-12728017 None:intergenic 30.0%
!! TTATTGTTGTTGTTTGCCTT+TGG - chr2_4:12728058-12728077 None:intergenic 30.0%
AAGAACATGCAAATTGACAA+TGG + chr2_4:12728106-12728125 Msa0315980:CDS 30.0%
ATGCAAATTGACAATGGAAA+AGG + chr2_4:12728112-12728131 Msa0315980:CDS 30.0%
!!! TTTGTATTTTGATTTGGTGG+TGG - chr2_4:12728343-12728362 None:intergenic 30.0%
!!! GTGTTTGTATTTTGATTTGG+TGG - chr2_4:12728346-12728365 None:intergenic 30.0%
GAAAAATCATCATACTCTTC+AGG - chr2_4:12728391-12728410 None:intergenic 30.0%
GGTAGTTTCATCTTATTCAA+AGG - chr2_4:12728475-12728494 None:intergenic 30.0%
GAATAAGATGAAACTACCTA+TGG + chr2_4:12728477-12728496 Msa0315980:CDS 30.0%
ATAAGATGAAACTACCTATG+GGG + chr2_4:12728479-12728498 Msa0315980:CDS 30.0%
! ATGATGATGAATGCTCAAAA+GGG + chr2_4:12728520-12728539 Msa0315980:CDS 30.0%
AATAATCAGAGTCAGAATCA+AGG + chr2_4:12728676-12728695 Msa0315980:CDS 30.0%
!! TTTTTACCCCCATTGTTATT+TGG - chr2_4:12728808-12728827 None:intergenic 30.0%
AAATAACAATGGGGGTAAAA+AGG + chr2_4:12728807-12728826 Msa0315980:CDS 30.0%
GTTCAAGGTATGATGAATAA+TGG + chr2_4:12728853-12728872 Msa0315980:CDS 30.0%
!! AATAATGGACTTCCAAACAT+GGG + chr2_4:12728868-12728887 Msa0315980:CDS 30.0%
TCATATTGTTAGAAACCATG+TGG - chr2_4:12728915-12728934 None:intergenic 30.0%
AATATGAATGGTATGCCAAT+GGG + chr2_4:12728928-12728947 Msa0315980:CDS 30.0%
! TGTATTGTTGTTGTTGGTAT+GGG - chr2_4:12729107-12729126 None:intergenic 30.0%
! ATGTATTGTTGTTGTTGGTA+TGG - chr2_4:12729108-12729127 None:intergenic 30.0%
ATACCAACAACAACAATACA+TGG + chr2_4:12729107-12729126 Msa0315980:CDS 30.0%
TGCAATGATGAATCAACAAA+GGG + chr2_4:12729131-12729150 Msa0315980:CDS 30.0%
TTGAAATCTATCATTTCCAC+CGG - chr2_4:12729166-12729185 None:intergenic 30.0%
TACATGTAATTAACACCCAT+TGG - chr2_4:12729210-12729229 None:intergenic 30.0%
! ATTTTCGTCGTTGAAAAAGT+TGG - chr2_4:12729286-12729305 None:intergenic 30.0%
GAAGAAATGAGATGATGAAT+TGG + chr2_4:12729328-12729347 Msa0315980:exon 30.0%
CTCATTTCTTTCAAAACTCA+AGG + chr2_4:12729614-12729633 Msa0315980:three_prime_UTR 30.0%
CCCATAAATAATGGCACAAA+AGG + chr2_4:12726605-12726624 Msa0315980:five_prime_UTR 35.0%
! ATGAGAAATGTGAGGCATTA+GGG - chr2_4:12726730-12726749 None:intergenic 35.0%
AGAGAAAGATGAGAAATGTG+AGG - chr2_4:12726738-12726757 None:intergenic 35.0%
GAGTTAGAGAGAAAAATGGT+GGG - chr2_4:12726777-12726796 None:intergenic 35.0%
CTTGGAGTTAGAGAGAAAAA+TGG - chr2_4:12726781-12726800 None:intergenic 35.0%
CAATGGGAGAAAAGAAAGAA+GGG - chr2_4:12726840-12726859 None:intergenic 35.0%
!!! ATGAAGGTTTTGGAAGCAAT+GGG - chr2_4:12726856-12726875 None:intergenic 35.0%
GAAAGGTTGAGAACATAACT+AGG - chr2_4:12726934-12726953 None:intergenic 35.0%
AAAGAGGGAGAAATGTAGAA+AGG - chr2_4:12726951-12726970 None:intergenic 35.0%
! TCAAAGGGTTCTAACAAAGA+GGG - chr2_4:12726966-12726985 None:intergenic 35.0%
! ATCAAAGGGTTCTAACAAAG+AGG - chr2_4:12726967-12726986 None:intergenic 35.0%
AAAGTGAACATACACTGTGA+CGG + chr2_4:12727253-12727272 Msa0315980:CDS 35.0%
AAGTTGGAAGAGTGAATATG+AGG - chr2_4:12727330-12727349 None:intergenic 35.0%
CAAACTGTGACGATATGAAT+TGG - chr2_4:12727508-12727527 None:intergenic 35.0%
TACAAACAGTAGTGAGAGAA+TGG - chr2_4:12727533-12727552 None:intergenic 35.0%
! TATTGGTAACAGTGAGCATA+TGG + chr2_4:12727578-12727597 Msa0315980:intron 35.0%
ATCAAGAAGCTTGCAAAATC+AGG + chr2_4:12728007-12728026 Msa0315980:CDS 35.0%
TCAAGAAGCTTGCAAAATCA+GGG + chr2_4:12728008-12728027 Msa0315980:CDS 35.0%
!! TTTGATTTGCAACGTTGTTC+TGG - chr2_4:12728084-12728103 None:intergenic 35.0%
CAAATTGACAATGGAAAAGG+TGG + chr2_4:12728115-12728134 Msa0315980:CDS 35.0%
TGGAGGAAACAAAAACAACA+AGG + chr2_4:12728138-12728157 Msa0315980:CDS 35.0%
GGAGGAAACAAAAACAACAA+GGG + chr2_4:12728139-12728158 Msa0315980:CDS 35.0%
GAGGAAACAAAAACAACAAG+GGG + chr2_4:12728140-12728159 Msa0315980:CDS 35.0%
AAAAACAACAAGGGGCAAAA+GGG + chr2_4:12728148-12728167 Msa0315980:CDS 35.0%
!!! TGCTGATTTTGGTTATTTCC+AGG - chr2_4:12728175-12728194 None:intergenic 35.0%
TAAAGGATTTGGAGATCTGA+AGG + chr2_4:12728285-12728304 Msa0315980:CDS 35.0%
ATGTTCATGTCCTTGAATTG+AGG - chr2_4:12728313-12728332 None:intergenic 35.0%
TAAGATGAAACTACCTATGG+GGG + chr2_4:12728480-12728499 Msa0315980:CDS 35.0%
! CATGATGATGAATGCTCAAA+AGG + chr2_4:12728519-12728538 Msa0315980:CDS 35.0%
AAGAACAATGGTGGCAAAAA+TGG + chr2_4:12728709-12728728 Msa0315980:CDS 35.0%
!! TTATTCCCATTTTTACCCTC+CGG - chr2_4:12728745-12728764 None:intergenic 35.0%
AATGGGAATAAGAATGTTGG+TGG + chr2_4:12728754-12728773 Msa0315980:CDS 35.0%
GTGGGATTCCAAATAACAAT+GGG + chr2_4:12728797-12728816 Msa0315980:CDS 35.0%
TGGGATTCCAAATAACAATG+GGG + chr2_4:12728798-12728817 Msa0315980:CDS 35.0%
AATGGGGGTAAAAAGGTAAA+TGG + chr2_4:12728814-12728833 Msa0315980:CDS 35.0%
ATGGGGGTAAAAAGGTAAAT+GGG + chr2_4:12728815-12728834 Msa0315980:CDS 35.0%
GGTAAAAAGGTAAATGGGAT+GGG + chr2_4:12728820-12728839 Msa0315980:CDS 35.0%
!! GAATAATGGACTTCCAAACA+TGG + chr2_4:12728867-12728886 Msa0315980:CDS 35.0%
CAATATGAATGGTATGCCAA+TGG + chr2_4:12728927-12728946 Msa0315980:CDS 35.0%
CTGCAATGATGAATCAACAA+AGG + chr2_4:12729130-12729149 Msa0315980:CDS 35.0%
ATGTAATTAACACCCATTGG+TGG - chr2_4:12729207-12729226 None:intergenic 35.0%
TCGTTGAAAAAGTTGGAGTA+TGG - chr2_4:12729279-12729298 None:intergenic 35.0%
AAGAAAAAAATGGGGGAGAA+TGG + chr2_4:12729564-12729583 Msa0315980:three_prime_UTR 35.0%
AGAAAAAAATGGGGGAGAAT+GGG + chr2_4:12729565-12729584 Msa0315980:three_prime_UTR 35.0%
AAAGTTTCCCAAGACATCCA+TGG + chr2_4:12726566-12726585 Msa0315980:five_prime_UTR 40.0%
TTAGCAACCATGGATGTCTT+GGG - chr2_4:12726576-12726595 None:intergenic 40.0%
TTTAGCAACCATGGATGTCT+TGG - chr2_4:12726577-12726596 None:intergenic 40.0%
GGGTATGTATTTAGCAACCA+TGG - chr2_4:12726586-12726605 None:intergenic 40.0%
AGGGAGAAGATGAACAGAAA+GGG - chr2_4:12726711-12726730 None:intergenic 40.0%
TAGGGAGAAGATGAACAGAA+AGG - chr2_4:12726712-12726731 None:intergenic 40.0%
! GATGAGAAATGTGAGGCATT+AGG - chr2_4:12726731-12726750 None:intergenic 40.0%
GGAGTTAGAGAGAAAAATGG+TGG - chr2_4:12726778-12726797 None:intergenic 40.0%
GTGTGTGAATATAAGCAAGG+GGG - chr2_4:12726818-12726837 None:intergenic 40.0%
GGTGTGTGAATATAAGCAAG+GGG - chr2_4:12726819-12726838 None:intergenic 40.0%
GGGTGTGTGAATATAAGCAA+GGG - chr2_4:12726820-12726839 None:intergenic 40.0%
AGGGTGTGTGAATATAAGCA+AGG - chr2_4:12726821-12726840 None:intergenic 40.0%
GCAATGGGAGAAAAGAAAGA+AGG - chr2_4:12726841-12726860 None:intergenic 40.0%
!!! GATGAAGGTTTTGGAAGCAA+TGG - chr2_4:12726857-12726876 None:intergenic 40.0%
TGTGACGATATGAATTGGCA+AGG - chr2_4:12727503-12727522 None:intergenic 40.0%
ACTGAGATAGATGCTGAACA+AGG + chr2_4:12727944-12727963 Msa0315980:CDS 40.0%
CTGAGATAGATGCTGAACAA+GGG + chr2_4:12727945-12727964 Msa0315980:CDS 40.0%
AGGGAAACATGCAGAACTTT+GGG + chr2_4:12728027-12728046 Msa0315980:CDS 40.0%
ATTGACAATGGAAAAGGTGG+TGG + chr2_4:12728118-12728137 Msa0315980:CDS 40.0%
CAAAAACAACAAGGGGCAAA+AGG + chr2_4:12728147-12728166 Msa0315980:CDS 40.0%
AACCAAAATCAGCAAAAGGG+TGG + chr2_4:12728181-12728200 Msa0315980:CDS 40.0%
! AGCTGTTGTTGTTGTTGTTG+AGG - chr2_4:12728226-12728245 None:intergenic 40.0%
TCAACAACAGTTACAACAGC+TGG + chr2_4:12728246-12728265 Msa0315980:CDS 40.0%
! TGATGATGAGTATTCCGATG+AGG + chr2_4:12728426-12728445 Msa0315980:CDS 40.0%
CTCATCATCAAACTCCTCAT+CGG - chr2_4:12728443-12728462 None:intergenic 40.0%
!! TGAGGAGTTTGATGATGAGA+TGG + chr2_4:12728444-12728463 Msa0315980:CDS 40.0%
AAGATGAAACTACCTATGGG+GGG + chr2_4:12728481-12728500 Msa0315980:CDS 40.0%
! ATGAATGCTCAAAAGGGTGT+TGG + chr2_4:12728526-12728545 Msa0315980:CDS 40.0%
AATGGGAATGGAGGAAAGAA+AGG + chr2_4:12728634-12728653 Msa0315980:CDS 40.0%
AATCAGAGTCAGAATCAAGG+TGG + chr2_4:12728679-12728698 Msa0315980:CDS 40.0%
GGTGGAGGTAATAAGAACAA+TGG + chr2_4:12728697-12728716 Msa0315980:CDS 40.0%
GGAGGTAATAAGAACAATGG+TGG + chr2_4:12728700-12728719 Msa0315980:CDS 40.0%
AACAATGGTGGCAAAAATGG+AGG + chr2_4:12728712-12728731 Msa0315980:CDS 40.0%
! AATAAGAATGTTGGTGGAGG+TGG + chr2_4:12728760-12728779 Msa0315980:CDS 40.0%
GGTGGGATTCCAAATAACAA+TGG + chr2_4:12728796-12728815 Msa0315980:CDS 40.0%
GGGATTCCAAATAACAATGG+GGG + chr2_4:12728799-12728818 Msa0315980:CDS 40.0%
GGGTAAAAAGGTAAATGGGA+TGG + chr2_4:12728819-12728838 Msa0315980:CDS 40.0%
GTGCCAATGATGAGTCAAAT+GGG + chr2_4:12728973-12728992 Msa0315980:CDS 40.0%
TTTCCACCCATCATCATTTC+AGG - chr2_4:12729084-12729103 None:intergenic 40.0%
! CAGCCATGTATTGTTGTTGT+TGG - chr2_4:12729113-12729132 None:intergenic 40.0%
ATTTACGCAAGACCACCAAT+GGG + chr2_4:12729192-12729211 Msa0315980:CDS 40.0%
TATGGATCTTGAGGATGTTG+AGG - chr2_4:12729261-12729280 None:intergenic 40.0%
AAGTTGGAGTATGGATCTTG+AGG - chr2_4:12729270-12729289 None:intergenic 40.0%
!! AGATGATGAATTGGTGATGC+TGG + chr2_4:12729337-12729356 Msa0315980:three_prime_UTR 40.0%
GAAAAAAATGGGGGAGAATG+GGG + chr2_4:12729566-12729585 Msa0315980:three_prime_UTR 40.0%
AGAGAGAGAGAGAGATTGAG+GGG - chr2_4:12726662-12726681 None:intergenic 45.0%
GAGAGAGAGAGAGAGATTGA+GGG - chr2_4:12726663-12726682 None:intergenic 45.0%
AGAGAGAGAGAGAGAGATTG+AGG - chr2_4:12726664-12726683 None:intergenic 45.0%
GGGAGAAGATGAACAGAAAG+GGG - chr2_4:12726710-12726729 None:intergenic 45.0%
TGCAGAGTAGAAAAGTCAGG+TGG - chr2_4:12727480-12727499 None:intergenic 45.0%
AGGTGCAGAGTAGAAAAGTC+AGG - chr2_4:12727483-12727502 None:intergenic 45.0%
GATAGATGCTGAACAAGGGA+AGG + chr2_4:12727949-12727968 Msa0315980:CDS 45.0%
TGGGTCCACATTTCCAGAAA+CGG - chr2_4:12727978-12727997 None:intergenic 45.0%
CAGGGAAACATGCAGAACTT+TGG + chr2_4:12728026-12728045 Msa0315980:CDS 45.0%
GGGAAACATGCAGAACTTTG+GGG + chr2_4:12728028-12728047 Msa0315980:CDS 45.0%
!!! CACCACCCTTTTGCTGATTT+TGG - chr2_4:12728186-12728205 None:intergenic 45.0%
CAAAATCAGCAAAAGGGTGG+TGG + chr2_4:12728184-12728203 Msa0315980:CDS 45.0%
! GTTGAGGATTTTGCCCTTGT+TGG - chr2_4:12728210-12728229 None:intergenic 45.0%
!! TCTGAAGGTGCCTCAATTCA+AGG + chr2_4:12728300-12728319 Msa0315980:CDS 45.0%
AAACTACCTATGGGGGGTAA+TGG + chr2_4:12728487-12728506 Msa0315980:CDS 45.0%
GCATTCATCATCATGTGAGC+AGG - chr2_4:12728514-12728533 None:intergenic 45.0%
!! GCTCAAAAGGGTGTTGGAAA+TGG + chr2_4:12728532-12728551 Msa0315980:CDS 45.0%
!! CAAAAGGGTGTTGGAAATGG+CGG + chr2_4:12728535-12728554 Msa0315980:CDS 45.0%
! AATGGAAAGAAAGGTGGTGG+CGG + chr2_4:12728562-12728581 Msa0315980:CDS 45.0%
! CCTGTTCAAGTTCATGGCTT+GGG + chr2_4:12728604-12728623 Msa0315980:CDS 45.0%
AATGGAGGAAAGAAAGGTGG+TGG + chr2_4:12728640-12728659 Msa0315980:CDS 45.0%
AATGGTGGCAAAAATGGAGG+TGG + chr2_4:12728715-12728734 Msa0315980:CDS 45.0%
CAAAAATGGAGGTGGAATGC+CGG + chr2_4:12728723-12728742 Msa0315980:CDS 45.0%
GAATGCCGGAGGGTAAAAAT+GGG + chr2_4:12728737-12728756 Msa0315980:CDS 45.0%
! GGGAATAAGAATGTTGGTGG+AGG + chr2_4:12728757-12728776 Msa0315980:CDS 45.0%
GTAAATGGGATGGGAGAAAG+TGG + chr2_4:12728829-12728848 Msa0315980:CDS 45.0%
ATGGGAGAAAGTGGTGTTCA+AGG + chr2_4:12728838-12728857 Msa0315980:CDS 45.0%
! AATGGACTTCCAAACATGGG+TGG + chr2_4:12728871-12728890 Msa0315980:CDS 45.0%
ATGAATGGTATGCCAATGGG+AGG + chr2_4:12728931-12728950 Msa0315980:CDS 45.0%
CGTGCCAATGATGAGTCAAA+TGG + chr2_4:12728972-12728991 Msa0315980:CDS 45.0%
CCTCCCATTTGACTCATCAT+TGG - chr2_4:12728979-12728998 None:intergenic 45.0%
CCAATGATGAGTCAAATGGG+AGG + chr2_4:12728976-12728995 Msa0315980:CDS 45.0%
TGGTGGTCCTGAAATGATGA+TGG + chr2_4:12729074-12729093 Msa0315980:CDS 45.0%
GGTGGTCCTGAAATGATGAT+GGG + chr2_4:12729075-12729094 Msa0315980:CDS 45.0%
AATCTATCATTTCCACCGGC+TGG - chr2_4:12729162-12729181 None:intergenic 45.0%
! GGTGGTCTTGCGTAAATCAT+TGG - chr2_4:12729189-12729208 None:intergenic 45.0%
GATTTACGCAAGACCACCAA+TGG + chr2_4:12729191-12729210 Msa0315980:CDS 45.0%
!! TAAAATTTAAAAGAAAAAAA+TGG + chr2_4:12729554-12729573 Msa0315980:three_prime_UTR 5.0%
!! AAAATTTAAAAGAAAAAAAT+GGG + chr2_4:12729555-12729574 Msa0315980:three_prime_UTR 5.0%
GAGAGAGAGAGAGATTGAGG+GGG - chr2_4:12726661-12726680 None:intergenic 50.0%
! GGTGACCGTTTCTGGAAATG+TGG + chr2_4:12727970-12727989 Msa0315980:CDS 50.0%
!! AGAACTTTGGGGTGCTCCAA+AGG + chr2_4:12728039-12728058 Msa0315980:CDS 50.0%
! GACAATGGAAAAGGTGGTGG+AGG + chr2_4:12728121-12728140 Msa0315980:CDS 50.0%
! AAAAGGGTGGTGGCCAACAA+GGG + chr2_4:12728194-12728213 Msa0315980:CDS 50.0%
GGAGGCAATGGAAAGAAAGG+TGG + chr2_4:12728556-12728575 Msa0315980:CDS 50.0%
GGCAATGGAAAGAAAGGTGG+TGG + chr2_4:12728559-12728578 Msa0315980:CDS 50.0%
CCATGAACTTGAACAGGCAC+AGG - chr2_4:12728601-12728620 None:intergenic 50.0%
! CCTGTGCCTGTTCAAGTTCA+TGG + chr2_4:12728598-12728617 Msa0315980:CDS 50.0%
! GCCTGTTCAAGTTCATGGCT+TGG + chr2_4:12728603-12728622 Msa0315980:CDS 50.0%
CCCAAGCCATGAACTTGAAC+AGG - chr2_4:12728607-12728626 None:intergenic 50.0%
! GTTCAAGTTCATGGCTTGGG+TGG + chr2_4:12728607-12728626 Msa0315980:CDS 50.0%
!! ATGGCTTGGGTGGTGGAAAT+GGG + chr2_4:12728617-12728636 Msa0315980:CDS 50.0%
!! TTGGGTGGTGGAAATGGGAA+TGG + chr2_4:12728622-12728641 Msa0315980:CDS 50.0%
GGGAATGGAGGAAAGAAAGG+TGG + chr2_4:12728637-12728656 Msa0315980:CDS 50.0%
CAGAGTCAGAATCAAGGTGG+AGG + chr2_4:12728682-12728701 Msa0315980:CDS 50.0%
GGAATGCCGGAGGGTAAAAA+TGG + chr2_4:12728736-12728755 Msa0315980:CDS 50.0%
! AAGAATGTTGGTGGAGGTGG+AGG + chr2_4:12728763-12728782 Msa0315980:CDS 50.0%
! GGTGGTGGTCATCCTAATGT+GGG + chr2_4:12728889-12728908 Msa0315980:CDS 50.0%
TCATCCTAATGTGGGCCACA+TGG + chr2_4:12728897-12728916 Msa0315980:CDS 50.0%
GAAACCATGTGGCCCACATT+AGG - chr2_4:12728904-12728923 None:intergenic 50.0%
AATGGTATGCCAATGGGAGG+AGG + chr2_4:12728934-12728953 Msa0315980:CDS 50.0%
GGTAACATGCCAGCTGTTCA+AGG + chr2_4:12728997-12729016 Msa0315980:CDS 50.0%
!! GGTCCTGAAATGATGATGGG+TGG + chr2_4:12729078-12729097 Msa0315980:CDS 50.0%
CAACAAAGGGCAATACCAGC+CGG + chr2_4:12729144-12729163 Msa0315980:CDS 50.0%
TCTTGAGGATGTTGAGGTGG+AGG - chr2_4:12729255-12729274 None:intergenic 50.0%
GGATCTTGAGGATGTTGAGG+TGG - chr2_4:12729258-12729277 None:intergenic 50.0%
GAGATTGAGGGGGAGAGAGA+TGG - chr2_4:12726651-12726670 None:intergenic 55.0%
GGGGTGTGAAGAAGAAGGCT+TGG - chr2_4:12726799-12726818 None:intergenic 55.0%
! GCAAGGGGGTGTGAAGAAGA+AGG - chr2_4:12726804-12726823 None:intergenic 55.0%
CAAGGGAAGGTGACCGTTTC+TGG + chr2_4:12727962-12727981 Msa0315980:CDS 55.0%
AACAAGGGGCAAAAGGGTCC+TGG + chr2_4:12728154-12728173 Msa0315980:CDS 55.0%
! CAAAAGGGTGGTGGCCAACA+AGG + chr2_4:12728193-12728212 Msa0315980:CDS 55.0%
!! AAGGGTGTTGGAAATGGCGG+AGG + chr2_4:12728538-12728557 Msa0315980:CDS 55.0%
! GTTGGAAATGGCGGAGGCAA+TGG + chr2_4:12728544-12728563 Msa0315980:CDS 55.0%
GGCGGAGGCAATGGAAAGAA+AGG + chr2_4:12728553-12728572 Msa0315980:CDS 55.0%
! CAAGTTCATGGCTTGGGTGG+TGG + chr2_4:12728610-12728629 Msa0315980:CDS 55.0%
!! CATGGCTTGGGTGGTGGAAA+TGG + chr2_4:12728616-12728635 Msa0315980:CDS 55.0%
!! GGTGGTGGAAATGGGAATGG+AGG + chr2_4:12728625-12728644 Msa0315980:CDS 55.0%
! GGAGGAAAGAAAGGTGGTGG+CGG + chr2_4:12728643-12728662 Msa0315980:CDS 55.0%
AAATGGAGGTGGAATGCCGG+AGG + chr2_4:12728726-12728745 Msa0315980:CDS 55.0%
AATGGAGGTGGAATGCCGGA+GGG + chr2_4:12728727-12728746 Msa0315980:CDS 55.0%
! AATGTTGGTGGAGGTGGAGG+TGG + chr2_4:12728766-12728785 Msa0315980:CDS 55.0%
!! GTGGAGGTGGTGGTAATGGT+GGG + chr2_4:12728779-12728798 Msa0315980:CDS 55.0%
GGACTTCCAAACATGGGTGG+TGG + chr2_4:12728874-12728893 Msa0315980:CDS 55.0%
GGATGACCACCACCCATGTT+TGG - chr2_4:12728883-12728902 None:intergenic 55.0%
! GGGTGGTGGTCATCCTAATG+TGG + chr2_4:12728888-12728907 Msa0315980:CDS 55.0%
CCAATGGGAGGAGGAGCAAT+GGG + chr2_4:12728943-12728962 Msa0315980:CDS 55.0%
!! GGTGTCAGTGGTGGATATGG+TGG + chr2_4:12729057-12729076 Msa0315980:CDS 55.0%
GGTGGAGGAGGGTAGCTATA+CGG - chr2_4:12729240-12729259 None:intergenic 55.0%
GAGGATGTTGAGGTGGAGGA+GGG - chr2_4:12729251-12729270 None:intergenic 55.0%
TGAGGATGTTGAGGTGGAGG+AGG - chr2_4:12729252-12729271 None:intergenic 55.0%
GCAGGACCATTACCCCCCAT+AGG - chr2_4:12728496-12728515 None:intergenic 60.0%
!! AAGAAAGGTGGTGGCGGCAG+TGG + chr2_4:12728568-12728587 Msa0315980:CDS 60.0%
!! GGAAAGAAAGGTGGTGGCGG+TGG + chr2_4:12728565-12728584 Msa0315980:CDS 60.0%
!! AAGAAAGGTGGTGGCGGTGG+CGG + chr2_4:12728649-12728668 Msa0315980:CDS 60.0%
! GGAGGTGGAGGTGGTGGTAA+TGG + chr2_4:12728775-12728794 Msa0315980:CDS 60.0%
!! GGTGGAGGTGGTGGTAATGG+TGG + chr2_4:12728778-12728797 Msa0315980:CDS 60.0%
GCCAATGGGAGGAGGAGCAA+TGG + chr2_4:12728942-12728961 Msa0315980:CDS 60.0%
CCCATTGCTCCTCCTCCCAT+TGG - chr2_4:12728946-12728965 None:intergenic 60.0%
GCCAGCTGTTCAAGGCCTAC+CGG + chr2_4:12729005-12729024 Msa0315980:CDS 60.0%
CTACCGGCAGCTGCGATGAA+CGG + chr2_4:12729021-12729040 Msa0315980:CDS 60.0%
!! GGCGGTGTCAGTGGTGGATA+TGG + chr2_4:12729054-12729073 Msa0315980:CDS 60.0%
CAAAGGGCAATACCAGCCGG+TGG + chr2_4:12729147-12729166 Msa0315980:CDS 60.0%
GGAGGAGGGTAGCTATACGG+CGG - chr2_4:12729237-12729256 None:intergenic 60.0%
!! AAAGGTGGTGGCGGCAGTGG+TGG + chr2_4:12728571-12728590 Msa0315980:CDS 65.0%
! GTTGGTGGAGGTGGAGGTGG+TGG + chr2_4:12728769-12728788 Msa0315980:CDS 65.0%
GCCGGTAGGCCTTGAACAGC+TGG - chr2_4:12729009-12729028 None:intergenic 65.0%
CGTTCATCGCAGCTGCCGGT+AGG - chr2_4:12729023-12729042 None:intergenic 65.0%
ATGAACGGCGGTGGAGGAGG+CGG + chr2_4:12729036-12729055 Msa0315980:CDS 65.0%
GGAGGGTAGCTATACGGCGG+AGG - chr2_4:12729234-12729253 None:intergenic 65.0%
!! AAAGGTGGTGGCGGTGGCGG+TGG + chr2_4:12728652-12728671 Msa0315980:CDS 70.0%
CCGCCGTTCATCGCAGCTGC+CGG - chr2_4:12729027-12729046 None:intergenic 70.0%
CCGGCAGCTGCGATGAACGG+CGG + chr2_4:12729024-12729043 Msa0315980:CDS 70.0%
GCAGCTGCGATGAACGGCGG+TGG + chr2_4:12729027-12729046 Msa0315980:CDS 70.0%
GCTGCGATGAACGGCGGTGG+AGG + chr2_4:12729030-12729049 Msa0315980:CDS 70.0%
GCGATGAACGGCGGTGGAGG+AGG + chr2_4:12729033-12729052 Msa0315980:CDS 70.0%
! GGTGGAGGAGGCGGTGTCAG+TGG + chr2_4:12729045-12729064 Msa0315980:CDS 70.0%
! GGAGGAGGCGGTGTCAGTGG+TGG + chr2_4:12729048-12729067 Msa0315980:CDS 70.0%
!! GGTGGTGGCGGCAGTGGTGG+AGG + chr2_4:12728574-12728593 Msa0315980:CDS 75.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 12726521 12729666 12726521 ID=Msa0315980;Name=Msa0315980
chr2_4 mRNA 12726521 12729666 12726521 ID=Msa0315980-mRNA-1;Parent=Msa0315980;Name=Msa0315980-mRNA-1;_AED=0.19;_eAED=0.19;_QI=475|1|1|1|1|1|3|337|500
chr2_4 exon 12726521 12727025 12726521 ID=Msa0315980-mRNA-1:exon:4856;Parent=Msa0315980-mRNA-1
chr2_4 exon 12727241 12727316 12727241 ID=Msa0315980-mRNA-1:exon:4857;Parent=Msa0315980-mRNA-1
chr2_4 exon 12727933 12729666 12727933 ID=Msa0315980-mRNA-1:exon:4858;Parent=Msa0315980-mRNA-1
chr2_4 five_prime_UTR 12726521 12726995 12726521 ID=Msa0315980-mRNA-1:five_prime_utr;Parent=Msa0315980-mRNA-1
chr2_4 CDS 12726996 12727025 12726996 ID=Msa0315980-mRNA-1:cds;Parent=Msa0315980-mRNA-1
chr2_4 CDS 12727241 12727316 12727241 ID=Msa0315980-mRNA-1:cds;Parent=Msa0315980-mRNA-1
chr2_4 CDS 12727933 12729329 12727933 ID=Msa0315980-mRNA-1:cds;Parent=Msa0315980-mRNA-1
chr2_4 three_prime_UTR 12729330 12729666 12729330 ID=Msa0315980-mRNA-1:three_prime_utr;Parent=Msa0315980-mRNA-1
Gene Sequence

>Msa0315980

ATGAGTAAAGAAGAGATTTTGAAGATACAGAAAAGTGTTCTCAAAGTGAACATACACTGTGACGGTTGCAAGCAGAAAGTTAAGAAAATCTTGCAGAAGATTGATGGGGTTTTTGCTACTGAGATAGATGCTGAACAAGGGAAGGTGACCGTTTCTGGAAATGTGGACCCAAATATTCTCATCAAGAAGCTTGCAAAATCAGGGAAACATGCAGAACTTTGGGGTGCTCCAAAGGCAAACAACAACAATAACAACCAGAACAACGTTGCAAATCAAATGAAGAACATGCAAATTGACAATGGAAAAGGTGGTGGAGGAAACAAAAACAACAAGGGGCAAAAGGGTCCTGGAAATAACCAAAATCAGCAAAAGGGTGGTGGCCAACAAGGGCAAAATCCTCAACAACAACAACAACAGCTTCAACAACAGTTACAACAGCTGGAACAACTTCAAAATATTAAAGGATTTGGAGATCTGAAGGTGCCTCAATTCAAGGACATGAACATGAAAATGCCACCACCAAATCAAAATACAAACACGAAAGGTGTTAAGTTCAATTTGCCTGAAGAGTATGATGATTTTTCTGATGATGAGTTTGATGATGATGAGTATTCCGATGAGGAGTTTGATGATGAGATGGAACATCCTTTGAATAAGATGAAACTACCTATGGGGGGTAATGGTCCTGCTCACATGATGATGAATGCTCAAAAGGGTGTTGGAAATGGCGGAGGCAATGGAAAGAAAGGTGGTGGCGGCAGTGGTGGAGGACCTGTGCCTGTTCAAGTTCATGGCTTGGGTGGTGGAAATGGGAATGGAGGAAAGAAAGGTGGTGGCGGTGGCGGTGGAAATAATCAGAGTCAGAATCAAGGTGGAGGTAATAAGAACAATGGTGGCAAAAATGGAGGTGGAATGCCGGAGGGTAAAAATGGGAATAAGAATGTTGGTGGAGGTGGAGGTGGTGGTAATGGTGGGATTCCAAATAACAATGGGGGTAAAAAGGTAAATGGGATGGGAGAAAGTGGTGTTCAAGGTATGATGAATAATGGACTTCCAAACATGGGTGGTGGTCATCCTAATGTGGGCCACATGGTTTCTAACAATATGAATGGTATGCCAATGGGAGGAGGAGCAATGGGAAACAACGTGCCAATGATGAGTCAAATGGGAGGTAACATGCCAGCTGTTCAAGGCCTACCGGCAGCTGCGATGAACGGCGGTGGAGGAGGCGGTGTCAGTGGTGGATATGGTGGTCCTGAAATGATGATGGGTGGAAACCCATACCAACAACAACAATACATGGCTGCAATGATGAATCAACAAAGGGCAATACCAGCCGGTGGAAATGATAGATTTCAACCAATGATTTACGCAAGACCACCAATGGGTGTTAATTACATGTATCCTCCGCCGTATAGCTACCCTCCTCCACCTCAACATCCTCAAGATCCATACTCCAACTTTTTCAACGACGAAAATACTTCAAGCTGCAGTGTTATGTGA
Protein sequence

>Msa0315980

MSKEEILKIQKSVLKVNIHCDGCKQKVKKILQKIDGVFATEIDAEQGKVTVSGNVDPNILIKKLAKSGKHAELWGAPKANNNNNNQNNVANQMKNMQIDNGKGGGGNKNNKGQKGPGNNQNQQKGGGQQGQNPQQQQQQLQQQLQQLEQLQNIKGFGDLKVPQFKDMNMKMPPPNQNTNTKGVKFNLPEEYDDFSDDEFDDDEYSDEEFDDEMEHPLNKMKLPMGGNGPAHMMMNAQKGVGNGGGNGKKGGGGSGGGPVPVQVHGLGGGNGNGGKKGGGGGGGNNQSQNQGGGNKNNGGKNGGGMPEGKNGNKNVGGGGGGGNGGIPNNNGGKKVNGMGESGVQGMMNNGLPNMGGGHPNVGHMVSNNMNGMPMGGGAMGNNVPMMSQMGGNMPAVQGLPAAAMNGGGGGGVSGGYGGPEMMMGGNPYQQQQYMAAMMNQQRAIPAGGNDRFQPMIYARPPMGVNYMYPPPYSYPPPPQHPQDPYSNFFNDENTSSCSVM*