AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1271910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1271910 MtrunA17_Chr8g0336161 91.577 463 22 5 1 451 1 458 0.0 707
Msa1271910 MtrunA17_Chr7g0233351 61.966 468 146 9 1 451 1 453 0.0 520
Msa1271910 MtrunA17_Chr4g0055371 52.298 457 164 11 2 442 5 423 4.75e-127 375
Msa1271910 MtrunA17_Chr5g0424111 56.376 298 107 4 144 437 155 433 4.62e-97 298
Msa1271910 MtrunA17_Chr3g0114791 49.041 365 145 8 103 437 68 421 3.64e-93 288
Msa1271910 MtrunA17_Chr8g0377191 39.785 186 112 0 181 366 3 188 5.41e-34 126
Msa1271910 MtrunA17_Chr8g0377671 37.755 196 122 0 171 366 1 196 1.25e-33 127
Msa1271910 MtrunA17_Chr8g0377671 38.272 162 100 0 186 347 108 269 1.56e-25 105
Msa1271910 MtrunA17_Chr1g0171491 31.884 207 136 2 173 379 45 246 4.91e-28 117
Msa1271910 MtrunA17_Chr8g0382971 36.517 178 113 0 188 365 69 246 7.43e-28 113
Msa1271910 MtrunA17_Chr8g0382971 35.028 177 110 2 186 360 113 286 2.26e-21 95.1
Msa1271910 MtrunA17_Chr8g0382971 33.083 133 85 2 236 366 25 155 1.91e-13 71.2
Msa1271910 MtrunA17_Chr8g0380791 33.333 180 120 0 187 366 10 189 9.58e-27 113
Msa1271910 MtrunA17_Chr8g0380791 35.036 137 89 0 230 366 7 143 6.15e-20 92.8
Msa1271910 MtrunA17_Chr8g0380791 38.596 114 70 0 190 303 82 195 6.94e-16 80.5
Msa1271910 MtrunA17_Chr4g0059911 32.258 217 139 2 185 401 51 259 1.66e-25 107
Msa1271910 MtrunA17_Chr4g0059911 36.413 184 116 1 192 374 11 194 1.43e-24 104
Msa1271910 MtrunA17_Chr4g0059911 33.333 132 87 1 236 366 9 140 2.05e-17 83.6
Msa1271910 MtrunA17_Chr8g0353121 30.263 228 151 2 165 387 23 247 5.26e-25 108
Msa1271910 MtrunA17_Chr3g0131001 37.209 172 108 0 191 362 56 227 5.69e-25 108
Msa1271910 MtrunA17_Chr3g0131001 38.281 128 79 0 238 365 57 184 8.82e-16 80.1
Msa1271910 MtrunA17_Chr3g0131001 38.235 102 62 1 187 288 144 244 2.84e-11 65.9
Msa1271910 MtrunA17_Chr5g0424301 35.928 167 107 0 187 353 66 232 2.32e-24 106
Msa1271910 MtrunA17_Chr5g0424301 39.062 128 78 0 238 365 71 198 1.51e-21 97.8
Msa1271910 MtrunA17_Chr5g0424301 36.792 106 67 0 261 366 71 176 8.89e-16 80.1
Msa1271910 MtrunA17_Chr8g0383331 37.838 148 92 0 189 336 3 150 2.66e-24 99.0
Msa1271910 MtrunA17_Chr8g0383331 39.062 128 78 0 234 361 2 129 3.86e-23 95.5
Msa1271910 MtrunA17_Chr8g0385241 36.126 191 105 4 189 365 124 311 3.33e-24 104
Msa1271910 MtrunA17_Chr8g0385241 30.986 213 113 3 188 366 54 266 5.67e-20 92.0
Msa1271910 MtrunA17_Chr8g0385241 32.620 187 116 1 188 364 8 194 4.72e-19 89.0
Msa1271910 MtrunA17_Chr8g0385241 34.884 172 109 2 184 352 175 346 2.33e-18 87.0
Msa1271910 MtrunA17_Chr8g0385241 26.042 192 132 1 185 366 28 219 9.03e-14 72.8
Msa1271910 MtrunA17_Chr7g0256301 31.319 182 123 1 174 353 53 234 4.07e-24 105
Msa1271910 MtrunA17_Chr3g0115111 34.759 187 121 1 191 376 58 244 5.25e-24 105
Msa1271910 MtrunA17_Chr3g0115111 33.065 124 83 0 189 312 103 226 1.01e-13 73.6
Msa1271910 MtrunA17_Chr8g0385201 35.119 168 108 1 184 350 24 191 4.08e-23 102
Msa1271910 MtrunA17_Chr8g0385201 38.596 114 70 0 252 365 47 160 1.11e-17 85.9
Msa1271910 MtrunA17_Chr8g0385201 33.645 107 70 1 261 366 32 138 3.16e-13 72.0
Msa1271910 MtrunA17_Chr3g0082501 31.746 189 125 1 187 375 61 245 6.29e-23 102
Msa1271910 MtrunA17_Chr8g0377211 42.857 119 68 0 229 347 35 153 4.54e-22 92.8
Msa1271910 MtrunA17_Chr8g0377211 33.333 132 65 1 241 372 24 132 1.61e-15 74.3
Msa1271910 MtrunA17_Chr1g0180261 33.721 172 91 1 218 366 56 227 1.03e-21 98.6
Msa1271910 MtrunA17_Chr1g0180261 29.592 196 113 2 178 348 37 232 3.27e-21 97.1
Msa1271910 MtrunA17_Chr1g0180261 34.848 132 86 0 234 365 49 180 8.68e-14 73.9
Msa1271910 MtrunA17_Chr1g0180261 36.735 98 62 0 206 303 136 233 6.51e-13 70.9
Msa1271910 MtrunA17_Chr2g0316611 31.771 192 108 1 190 358 105 296 6.59e-19 88.2
Msa1271910 MtrunA17_Chr2g0316611 32.402 179 121 0 185 363 31 209 1.64e-18 87.0
Msa1271910 MtrunA17_Chr2g0316611 30.612 196 131 1 168 363 157 347 9.16e-16 78.6
Msa1271910 MtrunA17_Chr2g0316631 31.122 196 112 2 187 359 33 228 2.08e-18 85.5
Msa1271910 MtrunA17_Chr2g0316631 30.114 176 123 0 190 365 105 280 1.04e-15 77.8
Msa1271910 MtrunA17_Chr2g0316631 31.579 171 117 0 188 358 126 296 1.87e-15 77.0
Msa1271910 MtrunA17_Chr2g0316631 28.846 156 111 0 188 343 149 304 7.65e-12 66.2
Msa1271910 MtrunA17_Chr6g0467061 32.768 177 119 0 184 360 13 189 9.72e-17 81.3
Msa1271910 MtrunA17_Chr6g0467061 31.771 192 108 1 190 358 88 279 4.13e-16 79.3
Msa1271910 MtrunA17_Chr6g0467061 34.815 135 88 0 230 364 13 147 5.61e-16 79.0
Msa1271910 MtrunA17_Chr6g0467061 32.386 176 119 0 188 363 155 330 3.72e-14 73.6
Msa1271910 MtrunA17_Chr8g0383551 29.121 182 84 4 218 379 105 261 7.11e-16 79.0
Msa1271910 MtrunA17_Chr8g0377221 33.544 158 95 2 186 342 70 218 8.58e-14 71.6
Msa1271910 MtrunA17_Chr8g0377221 23.913 230 119 4 188 370 3 223 1.75e-12 67.8
Msa1271910 MtrunA17_Chr1g0210831 32.836 134 90 0 232 365 609 742 3.97e-13 71.6
Msa1271910 MtrunA17_Chr1g0210831 32.800 125 84 0 216 340 616 740 5.74e-12 68.2
Msa1271910 MtrunA17_Chr5g0398011 34.862 109 71 0 238 346 37 145 2.32e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1271910 AT4G17080.1 58.311 379 132 9 86 439 128 505 2.78e-137 404
Msa1271910 AT2G35170.1 53.959 341 128 7 95 411 111 446 4.96e-117 352
Msa1271910 AT1G21920.1 55.873 315 115 5 136 437 107 410 5.87e-100 305
Msa1271910 AT1G21920.2 56.641 256 97 4 136 378 107 361 2.82e-94 289
Msa1271910 AT1G77660.1 51.212 330 134 3 128 441 100 418 2.67e-93 288
Msa1271910 AT3G56960.2 32.857 210 135 2 168 375 48 253 6.18e-30 123
Msa1271910 AT3G56960.1 32.857 210 135 2 168 375 48 253 6.18e-30 123
Msa1271910 AT2G41210.1 35.450 189 118 1 187 375 67 251 6.84e-30 123
Msa1271910 AT1G60890.1 37.778 180 112 0 187 366 8 187 1.57e-29 122
Msa1271910 AT1G60890.1 38.843 121 66 2 189 309 102 214 8.74e-14 74.3
Msa1271910 AT1G60890.1 37.168 113 71 0 191 303 81 193 2.85e-13 72.4
Msa1271910 AT1G60890.2 37.778 180 112 0 187 366 20 199 1.62e-29 122
Msa1271910 AT1G60890.2 38.843 121 66 2 189 309 114 226 9.71e-14 73.9
Msa1271910 AT1G60890.2 37.168 113 71 0 191 303 93 205 3.13e-13 72.4
Msa1271910 AT1G21980.1 33.014 209 130 2 152 360 48 246 1.76e-27 116
Msa1271910 AT1G21980.1 39.062 128 78 0 238 365 78 205 7.84e-22 99.0
Msa1271910 AT1G21980.1 36.792 106 67 0 261 366 78 183 1.50e-15 79.7
Msa1271910 AT3G07960.1 32.804 189 123 1 187 375 24 208 1.03e-26 114
Msa1271910 AT3G07960.3 32.804 189 123 1 187 375 24 208 1.18e-26 113
Msa1271910 AT3G07960.2 32.804 189 123 1 187 375 24 208 1.35e-26 113
Msa1271910 AT3G07960.4 32.804 189 123 1 187 375 24 208 1.35e-26 113
Msa1271910 AT1G10900.2 37.222 180 113 0 187 366 8 187 1.39e-26 113
Msa1271910 AT1G10900.2 31.111 135 93 0 231 365 6 140 8.47e-15 77.4
Msa1271910 AT1G10900.2 39.091 110 67 0 194 303 84 193 3.77e-13 72.0
Msa1271910 AT1G10900.2 40.000 90 54 0 189 278 102 191 1.41e-11 67.4
Msa1271910 AT1G10900.1 37.222 180 113 0 187 366 8 187 1.39e-26 113
Msa1271910 AT1G10900.1 31.111 135 93 0 231 365 6 140 8.47e-15 77.4
Msa1271910 AT1G10900.1 39.091 110 67 0 194 303 84 193 3.77e-13 72.0
Msa1271910 AT1G10900.1 40.000 90 54 0 189 278 102 191 1.41e-11 67.4
Msa1271910 AT1G10900.3 37.222 180 113 0 187 366 8 187 1.39e-26 113
Msa1271910 AT1G10900.3 31.111 135 93 0 231 365 6 140 8.47e-15 77.4
Msa1271910 AT1G10900.3 39.091 110 67 0 194 303 84 193 3.77e-13 72.0
Msa1271910 AT1G10900.3 40.000 90 54 0 189 278 102 191 1.41e-11 67.4
Msa1271910 AT2G26420.2 34.595 185 119 1 178 360 39 223 2.79e-26 112
Msa1271910 AT2G26420.2 35.938 128 82 0 238 365 55 182 1.04e-17 86.3
Msa1271910 AT2G26420.2 33.962 106 70 0 261 366 55 160 2.78e-12 69.3
Msa1271910 AT2G26420.3 34.595 185 119 1 178 360 39 223 2.79e-26 112
Msa1271910 AT2G26420.3 35.938 128 82 0 238 365 55 182 1.04e-17 86.3
Msa1271910 AT2G26420.3 33.962 106 70 0 261 366 55 160 2.78e-12 69.3
Msa1271910 AT2G26420.1 34.595 185 119 1 178 360 39 223 3.70e-26 112
Msa1271910 AT2G26420.1 35.938 128 82 0 238 365 55 182 1.27e-17 86.3
Msa1271910 AT2G26420.1 33.962 106 70 0 261 366 55 160 3.33e-12 69.3
Msa1271910 AT1G77740.2 34.706 170 111 0 191 360 78 247 1.97e-24 107
Msa1271910 AT1G77740.2 39.844 128 77 0 238 365 79 206 5.62e-22 99.8
Msa1271910 AT1G77740.1 34.706 170 111 0 191 360 78 247 1.97e-24 107
Msa1271910 AT1G77740.1 39.844 128 77 0 238 365 79 206 5.62e-22 99.8
Msa1271910 AT3G09920.3 30.000 210 145 1 157 366 22 229 3.02e-23 103
Msa1271910 AT3G09920.3 35.185 108 70 0 261 368 55 162 1.28e-14 77.0
Msa1271910 AT3G09920.3 37.069 116 73 0 188 303 120 235 5.21e-14 75.1
Msa1271910 AT3G09920.4 30.000 210 145 1 157 366 22 229 3.02e-23 103
Msa1271910 AT3G09920.4 35.185 108 70 0 261 368 55 162 1.28e-14 77.0
Msa1271910 AT3G09920.4 37.069 116 73 0 188 303 120 235 5.21e-14 75.1
Msa1271910 AT3G09920.2 30.000 210 145 1 157 366 22 229 3.02e-23 103
Msa1271910 AT3G09920.2 35.185 108 70 0 261 368 55 162 1.28e-14 77.0
Msa1271910 AT3G09920.2 37.069 116 73 0 188 303 120 235 5.21e-14 75.1
Msa1271910 AT3G09920.1 30.000 210 145 1 157 366 22 229 3.02e-23 103
Msa1271910 AT3G09920.1 35.185 108 70 0 261 368 55 162 1.28e-14 77.0
Msa1271910 AT3G09920.1 37.069 116 73 0 188 303 120 235 5.21e-14 75.1
Msa1271910 AT1G75010.1 32.000 125 85 0 216 340 605 729 4.28e-11 65.9
Msa1271910 AT1G75010.1 34.286 105 69 0 259 363 602 706 4.51e-11 65.5

Find 142 sgRNAs with CRISPR-Local

Find 282 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCAATATTGTGGAAATT+AGG 0.064253 8_3:-5422135 Msa1271910:CDS
ATTGAAATTGAAATTGAAAT+TGG 0.184005 8_3:+5422477 None:intergenic
GGGGAATAGGGTTAGGGATT+TGG 0.223176 8_3:+5422498 None:intergenic
AACCCTAATTTCCACAATAT+TGG 0.227162 8_3:+5422132 None:intergenic
GAGTAAGGGTAGTAGGTATA+AGG 0.242613 8_3:-5421963 Msa1271910:CDS
ATCCAATATTGTGGAAATTA+GGG 0.251880 8_3:-5422134 Msa1271910:CDS
AGATTTGCAGCTAGCTATTC+TGG 0.254201 8_3:-5419226 Msa1271910:three_prime_UTR
TGAAATTGAAATTGGGGAAT+AGG 0.258224 8_3:+5422485 None:intergenic
CAGACAAGATGCATGGATTT+GGG 0.264590 8_3:-5420432 Msa1271910:CDS
TTGAAATTGAAATTGAAATT+GGG 0.281485 8_3:+5422478 None:intergenic
GAAATGGGTTTGGTGTTTAT+AGG 0.300633 8_3:-5421919 Msa1271910:CDS
TATGAGGATGAAAGAGATTA+GGG 0.301966 8_3:+5422602 None:intergenic
GCAGACAAGATGCATGGATT+TGG 0.305587 8_3:-5420433 Msa1271910:CDS
GAAATTGAAATTGGGGAATA+GGG 0.305926 8_3:+5422486 None:intergenic
TGGTGTTGAGAGTTGGAGTA+AGG 0.329589 8_3:-5421978 Msa1271910:CDS
TATGAAGGGGATTGGGTTGA+TGG 0.332229 8_3:-5422016 Msa1271910:CDS
GATGGGAAATATGAAGGTTT+TGG 0.332995 8_3:-5421998 Msa1271910:CDS
CTTGTTAATAAGATGTTCAT+TGG 0.357858 8_3:-5418871 Msa1271910:three_prime_UTR
TATAGAGATGGATTGAGAAA+TGG 0.358776 8_3:-5421935 Msa1271910:CDS
GTCTTGGCCACTATCACTTT+AGG 0.368209 8_3:-5421775 Msa1271910:intron
TTATGAGGATGAAAGAGATT+AGG 0.372460 8_3:+5422601 None:intergenic
TTGACACCAACAGGGGAAAC+TGG 0.381788 8_3:+5420253 None:intergenic
CGGGAGTGATTGACACCAAC+AGG 0.381949 8_3:+5420244 None:intergenic
AGAAGAAAGGGAGGGAAAGA+AGG 0.382410 8_3:+5422350 None:intergenic
TGAAATTGGGGAATAGGGTT+AGG 0.385743 8_3:+5422491 None:intergenic
TTACAACAGTAGCTGGGTTA+AGG 0.387908 8_3:+5418987 None:intergenic
ATCTACCTTGGCTACATCAT+AGG 0.396492 8_3:+5419432 None:intergenic
GATTTGTTGGTGAGTTTAAG+TGG 0.397616 8_3:-5421811 Msa1271910:CDS
ATATTGTGGAAATTAGGGTT+AGG 0.403227 8_3:-5422129 Msa1271910:CDS
AGAGTTGGAGTAAGGGTAGT+AGG 0.407656 8_3:-5421970 Msa1271910:CDS
ATAGAGATGGATTGAGAAAT+GGG 0.407814 8_3:-5421934 Msa1271910:CDS
AGGGTTAGGGTTTATGCAAA+TGG 0.414027 8_3:-5422115 Msa1271910:CDS
AGTAAGGGTAGTAGGTATAA+GGG 0.424632 8_3:-5421962 Msa1271910:CDS
AGTTGATTGCCTCACAAAAC+AGG 0.425609 8_3:+5419289 None:intergenic
TGGGTTGATGGGAAATATGA+AGG 0.426845 8_3:-5422004 Msa1271910:CDS
GATGATGGGAGTAGATTTGT+TGG 0.429175 8_3:-5421824 Msa1271910:CDS
AAAGAGATTAGGGTTGAAAT+CGG 0.437710 8_3:+5422612 None:intergenic
TGCCTCACAAAACAGGAATT+CGG 0.438059 8_3:+5419296 None:intergenic
ATTTGTTGGTGAGTTTAAGT+GGG 0.438217 8_3:-5421810 Msa1271910:CDS
CGGTGGTGATTTGAATTTGA+GGG 0.439339 8_3:+5422240 None:intergenic
GCAAATGGCCATCGTTATGA+AGG 0.442940 8_3:-5420397 Msa1271910:CDS
GAATAGGGTTAGGGATTTGG+AGG 0.443890 8_3:+5422501 None:intergenic
TATTGTGGAAATTAGGGTTA+GGG 0.446996 8_3:-5422128 Msa1271910:CDS
GTGGAAGATATGAAGGGGAT+TGG 0.450612 8_3:-5422024 Msa1271910:CDS
GGAATGTATACATTTAGAAA+TGG 0.453121 8_3:-5420343 Msa1271910:CDS
TGAAATTGAAATTGAAATTG+GGG 0.459705 8_3:+5422479 None:intergenic
GAAATTGGGGAATAGGGTTA+GGG 0.462785 8_3:+5422492 None:intergenic
TGGGATCTTACAACAGTAGC+TGG 0.464200 8_3:+5418980 None:intergenic
AATATGAGTGGAAGATATGA+AGG 0.464536 8_3:-5422031 Msa1271910:CDS
TGGAAGATATGAAGGGGATT+GGG 0.467735 8_3:-5422023 Msa1271910:CDS
AGTAGAGAAGCATGAGATTG+TGG 0.478242 8_3:-5419252 Msa1271910:three_prime_UTR
CCTGCACTGTATTAAGTACT+CGG 0.478839 8_3:+5420224 None:intergenic
GGTGTTGAGAGTTGGAGTAA+GGG 0.478917 8_3:-5421977 Msa1271910:CDS
GAGAAACTCAATCTGGTCAC+TGG 0.482465 8_3:-5420321 Msa1271910:CDS
TCAGCAACTGGTGGAGATGA+TGG 0.484429 8_3:+5419191 None:intergenic
TATACTGGGGATGTTTATGC+TGG 0.487144 8_3:-5421893 Msa1271910:CDS
GAGGGGAAAAGACATGGTCT+TGG 0.489572 8_3:-5420364 Msa1271910:CDS
AAACTCACCTAAAGTGATAG+TGG 0.490431 8_3:+5421768 None:intergenic
AGAAGAAAGAGTGAGTAGGT+GGG 0.490534 8_3:+5422314 None:intergenic
TGGGGTGTTAAACATGGTCT+TGG 0.492897 8_3:-5421791 Msa1271910:CDS
ACATATCCAGTTTCCCCTGT+TGG 0.495511 8_3:-5420259 Msa1271910:CDS
GGTGTTCATACTTGTGATGA+TGG 0.496834 8_3:-5421839 Msa1271910:CDS
CGGAGAAAGCGAAAGCAGAT+AGG 0.497218 8_3:+5422284 None:intergenic
ATTTACTCGTTCATCTACCT+TGG 0.497616 8_3:+5419420 None:intergenic
AAGAAGAAAGAGTGAGTAGG+TGG 0.498494 8_3:+5422313 None:intergenic
AATGAGAAGTGTTGTGGGAG+TGG 0.498578 8_3:-5422067 Msa1271910:CDS
TATGTGGCGTTCTGCGTGCT+TGG 0.500334 8_3:+5420277 None:intergenic
ATGAAGGGGATTGGGTTGAT+GGG 0.500799 8_3:-5422015 Msa1271910:CDS
AGCAGCTACTCTAGCTGCAT+TGG 0.500850 8_3:+5419369 None:intergenic
ATGAGAAGTGTTGTGGGAGT+GGG 0.501259 8_3:-5422066 Msa1271910:CDS
GCTTGCGTTCAGTCAGCAAC+TGG 0.502290 8_3:+5419179 None:intergenic
TATGCTGGTGAATGGTTGAA+TGG 0.503530 8_3:-5421878 Msa1271910:CDS
ATTGAATTGAAAAGTTTATG+AGG 0.504198 8_3:+5422586 None:intergenic
GATATTTGCTTTAACTGACT+TGG 0.507054 8_3:-5418916 Msa1271910:three_prime_UTR
TGTTTCACTGTGAGGACTAT+CGG 0.507879 8_3:+5422208 None:intergenic
GGTTGAATGGTCAGAGTCAT+GGG 0.508114 8_3:-5421865 Msa1271910:CDS
GGGAAAGAAGGAGGATTAGA+CGG 0.508839 8_3:+5422362 None:intergenic
GAAGAAAGAGTGAGTAGGTG+GGG 0.509004 8_3:+5422315 None:intergenic
AGAAATGGAGAAACTCAATC+TGG 0.509744 8_3:-5420328 Msa1271910:CDS
TCATGTAGAGTCACGTATGC+TGG 0.510542 8_3:-5419023 Msa1271910:three_prime_UTR
GTGGCGGAGACGGCGGAGTG+CGG 0.516080 8_3:+5422390 None:intergenic
GATGGATTGAGAAATGGGTT+TGG 0.521028 8_3:-5421929 Msa1271910:CDS
GGGATGTTTATGCTGGTGAA+TGG 0.523748 8_3:-5421886 Msa1271910:CDS
GCGGAGACGGCGGAGTGCGG+CGG 0.525703 8_3:+5422393 None:intergenic
GACGGAAACGGTGGCGGAGA+CGG 0.530033 8_3:+5422380 None:intergenic
TGAAGAATGAGAAGTGTTGT+GGG 0.537863 8_3:-5422072 Msa1271910:CDS
GCGGAGTGCGGCGGATGAGA+TGG 0.539666 8_3:+5422402 None:intergenic
GGATTAGACGGAAACGGTGG+CGG 0.540352 8_3:+5422374 None:intergenic
TATAAGGGTATGTATAGAGA+TGG 0.544385 8_3:-5421947 Msa1271910:CDS
GTGAAGAAATAGAATTGTGT+TGG 0.554593 8_3:+5422551 None:intergenic
AGAAAGGGAGGGAAAGAAGG+AGG 0.556007 8_3:+5422353 None:intergenic
TTTGTTGGTGAGTTTAAGTG+GGG 0.563464 8_3:-5421809 Msa1271910:CDS
TGGTTGAATGGTCAGAGTCA+TGG 0.568619 8_3:-5421866 Msa1271910:CDS
AGCTAGAGTAGCTGCTGTCA+AGG 0.569449 8_3:-5419362 Msa1271910:CDS
ACGGTGGTGATTTGAATTTG+AGG 0.571474 8_3:+5422239 None:intergenic
TGCGTTCAGTCAGCAACTGG+TGG 0.573564 8_3:+5419182 None:intergenic
GAAGGAGGATTAGACGGAAA+CGG 0.574183 8_3:+5422368 None:intergenic
AGAAATGGAGATACGTATGC+TGG 0.574773 8_3:-5420466 Msa1271910:CDS
AACAGGGGAAACTGGATATG+TGG 0.576987 8_3:+5420261 None:intergenic
GTTTATTACTATAATATGAG+TGG 0.579922 8_3:-5422043 Msa1271910:CDS
TGAGAAGTGTTGTGGGAGTG+GGG 0.582204 8_3:-5422065 Msa1271910:CDS
GCCATCGTTATGAAGGAACC+TGG 0.582273 8_3:-5420390 Msa1271910:CDS
ATGGGTCTTGTATAGAGAGA+GGG 0.583170 8_3:-5419136 Msa1271910:three_prime_UTR
TTTAAGTGGGGTGTTAAACA+TGG 0.583836 8_3:-5421797 Msa1271910:CDS
AGTCACGTATGCTGGTGTGC+TGG 0.584349 8_3:-5419015 Msa1271910:three_prime_UTR
ATATGAGTGGAAGATATGAA+GGG 0.589019 8_3:-5422030 Msa1271910:CDS
TTAAAGAAGAAAGAGTGAGT+AGG 0.590259 8_3:+5422310 None:intergenic
TATGAAGGAACCTGGCATGA+GGG 0.590379 8_3:-5420382 Msa1271910:CDS
CCGAGTACTTAATACAGTGC+AGG 0.602231 8_3:-5420224 Msa1271910:intron
ATGAAGGAACCTGGCATGAG+GGG 0.602811 8_3:-5420381 Msa1271910:CDS
ATGAAGAATGAGAAGTGTTG+TGG 0.603885 8_3:-5422073 Msa1271910:CDS
TATATTGCAGACAAGATGCA+TGG 0.607351 8_3:-5420439 Msa1271910:CDS
TGGCATGAGGGGAAAAGACA+TGG 0.607412 8_3:-5420370 Msa1271910:CDS
GCAAATGGTGATGTGTATGA+AGG 0.608050 8_3:-5422100 Msa1271910:CDS
GGAAACGGTGGCGGAGACGG+CGG 0.612548 8_3:+5422383 None:intergenic
TTATGAAGGAACCTGGCATG+AGG 0.612995 8_3:-5420383 Msa1271910:CDS
TTTGCAGCTAGCTATTCTGG+TGG 0.615642 8_3:-5419223 Msa1271910:three_prime_UTR
AGACAAGATGCATGGATTTG+GGG 0.616252 8_3:-5420431 Msa1271910:CDS
AGTTGCTGACTGAACGCAAG+CGG 0.616526 8_3:-5419177 Msa1271910:three_prime_UTR
GAGTGCGGCGGATGAGATGG+CGG 0.619453 8_3:+5422405 None:intergenic
CAGTGAAACAGTGGAATCGA+CGG 0.623242 8_3:-5422195 Msa1271910:CDS
GGGATCTTACAACAGTAGCT+GGG 0.624185 8_3:+5418981 None:intergenic
GTGTTCATACTTGTGATGAT+GGG 0.626133 8_3:-5421838 Msa1271910:CDS
GCCAGGTTCCTTCATAACGA+TGG 0.629240 8_3:+5420389 None:intergenic
TATGGGTCTTGTATAGAGAG+AGG 0.629329 8_3:-5419137 Msa1271910:three_prime_UTR
AGGACTATCGGAAGAGACGA+CGG 0.633764 8_3:+5422220 None:intergenic
GAAAGCCTATGATGTAGCCA+AGG 0.637335 8_3:-5419437 Msa1271910:CDS
AATAGGGTTAGGGATTTGGA+GGG 0.639807 8_3:+5422502 None:intergenic
GGGAGTGATTGACACCAACA+GGG 0.649159 8_3:+5420245 None:intergenic
CTGCACTGTATTAAGTACTC+GGG 0.651939 8_3:+5420225 None:intergenic
TATGAGTGGAAGATATGAAG+GGG 0.653354 8_3:-5422029 Msa1271910:CDS
AATGGTCAGAGTCATGGGAG+TGG 0.666277 8_3:-5421860 Msa1271910:CDS
TAGTCCTCACAGTGAAACAG+TGG 0.670821 8_3:-5422204 Msa1271910:CDS
GATTCCACTGTTTCACTGTG+AGG 0.673057 8_3:+5422200 None:intergenic
TCAAAACATCTTGCGCACGA+TGG 0.680906 8_3:+5418937 None:intergenic
AAGACAGAAGAAAAGTGCGG+CGG 0.688106 8_3:+5422264 None:intergenic
GGAGGATTAGACGGAAACGG+TGG 0.701072 8_3:+5422371 None:intergenic
GGAAAGACAGAAGAAAAGTG+CGG 0.713433 8_3:+5422261 None:intergenic
TGAAACAGTGGAATCGACGG+CGG 0.724376 8_3:-5422192 Msa1271910:CDS
GTCTTGTATAGAGAGAGGGA+AGG 0.736168 8_3:-5419132 Msa1271910:three_prime_UTR
GGAGTGATTGACACCAACAG+GGG 0.738177 8_3:+5420246 None:intergenic
ACTATCGGAAGAGACGACGG+TGG 0.753609 8_3:+5422223 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGATTTTATGATGAAT+TGG + chr8_3:5419096-5419115 None:intergenic 15.0%
!! TTGAAATTGAAATTGAAATT+GGG + chr8_3:5419147-5419166 None:intergenic 15.0%
!! ATTGAAATTGAAATTGAAAT+TGG + chr8_3:5419148-5419167 None:intergenic 15.0%
!! TATATTTGAGAAAAATAAGA+AGG + chr8_3:5420997-5421016 None:intergenic 15.0%
!!! TTCTTATTTTTCTCAAATAT+AGG - chr8_3:5420996-5421015 Msa1271910:intron 15.0%
!!! TATTTTCATTTCATTAGAAA+TGG - chr8_3:5421141-5421160 Msa1271910:intron 15.0%
!! AGTAATTCTTATAATTTGAT+AGG - chr8_3:5421604-5421623 Msa1271910:intron 15.0%
!! AAAAAATCAGAAATTCAGAT+CGG - chr8_3:5418964-5418983 Msa1271910:three_prime_UTR 20.0%
!! ATTGAATTGAAAAGTTTATG+AGG + chr8_3:5419039-5419058 None:intergenic 20.0%
!! TGAAATTGAAATTGAAATTG+GGG + chr8_3:5419146-5419165 None:intergenic 20.0%
!!! ATTTTTTAGTGGAAAATTGT+GGG + chr8_3:5419173-5419192 None:intergenic 20.0%
!!! ATGAAAAGATGATTTTTTAG+TGG + chr8_3:5419184-5419203 None:intergenic 20.0%
!! ATAGTAAAAAGAAGAAGAAA+GGG + chr8_3:5419287-5419306 None:intergenic 20.0%
!! GTTTATTACTATAATATGAG+TGG - chr8_3:5419579-5419598 Msa1271910:intron 20.0%
!! TAATATTGCATCTGTTTATT+GGG - chr8_3:5420514-5420533 Msa1271910:intron 20.0%
!! TTCACGTAAAGTATATTAAA+GGG + chr8_3:5420796-5420815 None:intergenic 20.0%
!!! ATTGTTGAATTGATTTATGT+AGG - chr8_3:5421053-5421072 Msa1271910:intron 20.0%
!!! TTGTTGAATTGATTTATGTA+GGG - chr8_3:5421054-5421073 Msa1271910:intron 20.0%
!!! TATAAGTATGACTCTATTTT+TGG - chr8_3:5421108-5421127 Msa1271910:intron 20.0%
!!! ATAAGTATGACTCTATTTTT+GGG - chr8_3:5421109-5421128 Msa1271910:intron 20.0%
!! TCAAATTATAAGAATTACTC+AGG + chr8_3:5421603-5421622 None:intergenic 20.0%
!! AAAATTACACTAAAACACAA+CGG + chr8_3:5421691-5421710 None:intergenic 20.0%
!!! ATTGGACATTTTTTTCTATA+TGG - chr8_3:5422020-5422039 Msa1271910:CDS 20.0%
!!! ATTTATTTTCTATCTGCTAT+GGG - chr8_3:5422468-5422487 Msa1271910:CDS 20.0%
!!! TCTCTTCTTATTTTTACTAT+GGG + chr8_3:5422664-5422683 None:intergenic 20.0%
!!! TTCTCTTCTTATTTTTACTA+TGG + chr8_3:5422665-5422684 None:intergenic 20.0%
!!! GATTTTTTAGTGGAAAATTG+TGG + chr8_3:5419174-5419193 None:intergenic 25.0%
! GATAGTAAAAAGAAGAAGAA+AGG + chr8_3:5419288-5419307 None:intergenic 25.0%
!!! GGATGAATTTTTCGAATTTT+TGG + chr8_3:5419472-5419491 None:intergenic 25.0%
! GAAAAATTCATCCAATATTG+TGG - chr8_3:5419479-5419498 Msa1271910:intron 25.0%
! ATCCAATATTGTGGAAATTA+GGG - chr8_3:5419488-5419507 Msa1271910:intron 25.0%
!!! GTGTTTATAGGTTTTATACT+GGG - chr8_3:5419715-5419734 Msa1271910:intron 25.0%
!!! TGTTTATAGGTTTTATACTG+GGG - chr8_3:5419716-5419735 Msa1271910:intron 25.0%
!! GTTTTGTGTTAATCAATGAT+TGG - chr8_3:5420148-5420167 Msa1271910:intron 25.0%
! GTTAATCAATGATTGGAATT+TGG - chr8_3:5420155-5420174 Msa1271910:intron 25.0%
! AATCTATTCCACCTAATATT+TGG + chr8_3:5420228-5420247 None:intergenic 25.0%
! AATATTAGGTGGAATAGATT+TGG - chr8_3:5420228-5420247 Msa1271910:CDS 25.0%
!! ATTTCAGATGTTAGGTTTTA+AGG - chr8_3:5420251-5420270 Msa1271910:CDS 25.0%
! TTAAGAATATAGTAGACAGA+TGG - chr8_3:5420446-5420465 Msa1271910:CDS 25.0%
! GTAATATTGCATCTGTTTAT+TGG - chr8_3:5420513-5420532 Msa1271910:intron 25.0%
! AATATTGCATCTGTTTATTG+GGG - chr8_3:5420515-5420534 Msa1271910:intron 25.0%
! AAAGTAACGAAAACAAAAAG+TGG + chr8_3:5420541-5420560 None:intergenic 25.0%
!!! TTTTCGTTACTTTATGTATC+CGG - chr8_3:5420548-5420567 Msa1271910:intron 25.0%
! TCACGTAAAGTATATTAAAG+GGG + chr8_3:5420795-5420814 None:intergenic 25.0%
! GTTCACGTAAAGTATATTAA+AGG + chr8_3:5420797-5420816 None:intergenic 25.0%
! TTTCAACAAAAAACCGAATA+GGG + chr8_3:5420885-5420904 None:intergenic 25.0%
!! TTTTCAACAAAAAACCGAAT+AGG + chr8_3:5420886-5420905 None:intergenic 25.0%
! ATTCAACAATAATGCACTAA+TGG + chr8_3:5421043-5421062 None:intergenic 25.0%
! TATACTATGGAGCAAATAAA+AGG + chr8_3:5421092-5421111 None:intergenic 25.0%
! ATAGAGTCATACTTATACTA+TGG + chr8_3:5421105-5421124 None:intergenic 25.0%
!!! TAAGTATGACTCTATTTTTG+GGG - chr8_3:5421110-5421129 Msa1271910:intron 25.0%
! GGAATGTATACATTTAGAAA+TGG - chr8_3:5421279-5421298 Msa1271910:intron 25.0%
! GAGCATTTAACAATAGATTA+AGG + chr8_3:5421450-5421469 None:intergenic 25.0%
!! TTTTCTTTTAGTGTTAGCTA+GGG + chr8_3:5421476-5421495 None:intergenic 25.0%
! CACATCATTCTAATTATTCT+AGG + chr8_3:5421548-5421567 None:intergenic 25.0%
! GCTATGTATTAAGGAAATAT+TGG - chr8_3:5421823-5421842 Msa1271910:CDS 25.0%
!! TTTTTGTTGGAAAGAAAAAG+TGG - chr8_3:5421886-5421905 Msa1271910:CDS 25.0%
!!! GATTTATTTTCTATCTGCTA+TGG - chr8_3:5422467-5422486 Msa1271910:CDS 25.0%
! ACATGAATTTACAAAAATGC+AGG + chr8_3:5422585-5422604 None:intergenic 25.0%
! CTTGTTAATAAGATGTTCAT+TGG - chr8_3:5422751-5422770 Msa1271910:five_prime_UTR 25.0%
! CTTCACTTTCTCTTTTTGTT+TGG + chr8_3:5418883-5418902 None:intergenic 30.0%
AAAGAGATTAGGGTTGAAAT+CGG + chr8_3:5419013-5419032 None:intergenic 30.0%
TATGAGGATGAAAGAGATTA+GGG + chr8_3:5419023-5419042 None:intergenic 30.0%
TTATGAGGATGAAAGAGATT+AGG + chr8_3:5419024-5419043 None:intergenic 30.0%
!!! TAGAATTGTGTTGGTTTTTG+TGG + chr8_3:5419065-5419084 None:intergenic 30.0%
GTGAAGAAATAGAATTGTGT+TGG + chr8_3:5419074-5419093 None:intergenic 30.0%
GAAATTGAAATTGGGGAATA+GGG + chr8_3:5419139-5419158 None:intergenic 30.0%
TGAAATTGAAATTGGGGAAT+AGG + chr8_3:5419140-5419159 None:intergenic 30.0%
TAAAAAGAAGAAGAAAGGGA+GGG + chr8_3:5419283-5419302 None:intergenic 30.0%
TTAAAGAAGAAAGAGTGAGT+AGG + chr8_3:5419315-5419334 None:intergenic 30.0%
CATCCAATATTGTGGAAATT+AGG - chr8_3:5419487-5419506 Msa1271910:intron 30.0%
AACCCTAATTTCCACAATAT+TGG + chr8_3:5419493-5419512 None:intergenic 30.0%
ATATTGTGGAAATTAGGGTT+AGG - chr8_3:5419493-5419512 Msa1271910:intron 30.0%
TATTGTGGAAATTAGGGTTA+GGG - chr8_3:5419494-5419513 Msa1271910:intron 30.0%
AATATGAGTGGAAGATATGA+AGG - chr8_3:5419591-5419610 Msa1271910:intron 30.0%
ATATGAGTGGAAGATATGAA+GGG - chr8_3:5419592-5419611 Msa1271910:intron 30.0%
! TATAAGGGTATGTATAGAGA+TGG - chr8_3:5419675-5419694 Msa1271910:intron 30.0%
!! TATAGAGATGGATTGAGAAA+TGG - chr8_3:5419687-5419706 Msa1271910:intron 30.0%
!! ATAGAGATGGATTGAGAAAT+GGG - chr8_3:5419688-5419707 Msa1271910:intron 30.0%
!! GGTGTTTATAGGTTTTATAC+TGG - chr8_3:5419714-5419733 Msa1271910:intron 30.0%
! ATTTGTTGGTGAGTTTAAGT+GGG - chr8_3:5419812-5419831 Msa1271910:intron 30.0%
TAAATTACTAATGGTGTAGC+CGG - chr8_3:5420014-5420033 Msa1271910:intron 30.0%
! TGTTTGTTGATATCAGTAGT+TGG - chr8_3:5420191-5420210 Msa1271910:intron 30.0%
AGATTTGGATTTCAGATGTT+AGG - chr8_3:5420243-5420262 Msa1271910:CDS 30.0%
!!! GTTTTAAGGATAAGTGAAGT+AGG - chr8_3:5420265-5420284 Msa1271910:CDS 30.0%
ACATGTCATAATAAGTTAGC+TGG + chr8_3:5420328-5420347 None:intergenic 30.0%
ATCTGTCTTTGATCATTCTT+TGG - chr8_3:5420349-5420368 Msa1271910:CDS 30.0%
! ACTTGCTGCTAATTTAAGTT+TGG - chr8_3:5420746-5420765 Msa1271910:intron 30.0%
CACGTAAAGTATATTAAAGG+GGG + chr8_3:5420794-5420813 None:intergenic 30.0%
CAGGATATAAAACAGGATAA+GGG + chr8_3:5420971-5420990 None:intergenic 30.0%
TCAGGATATAAAACAGGATA+AGG + chr8_3:5420972-5420991 None:intergenic 30.0%
AAGGATTCAGGATATAAAAC+AGG + chr8_3:5420978-5420997 None:intergenic 30.0%
GAGAAAAATAAGAAGGATTC+AGG + chr8_3:5420990-5421009 None:intergenic 30.0%
!!! AAGTATGACTCTATTTTTGG+GGG - chr8_3:5421111-5421130 Msa1271910:intron 30.0%
! CTTTTCTTTTAGTGTTAGCT+AGG + chr8_3:5421477-5421496 None:intergenic 30.0%
TTTCTCTTATGTCTACACAT+TGG - chr8_3:5421522-5421541 Msa1271910:intron 30.0%
!! GACTTGTTGAAAAATCTGAT+TGG - chr8_3:5421662-5421681 Msa1271910:intron 30.0%
TATGTGAAACTTGAAACAGA+TGG + chr8_3:5421754-5421773 None:intergenic 30.0%
CAGAACAATGCTATGTATTA+AGG - chr8_3:5421814-5421833 Msa1271910:CDS 30.0%
GAAATTTCGACAAAATGAGA+TGG + chr8_3:5421949-5421968 None:intergenic 30.0%
! TCTCATTTTGTCGAAATTTC+TGG - chr8_3:5421949-5421968 Msa1271910:CDS 30.0%
TTTGTAAAAGCTCGAGATTA+TGG - chr8_3:5421990-5422009 Msa1271910:CDS 30.0%
GATATTTGCTTTAACTGACT+TGG - chr8_3:5422706-5422725 Msa1271910:five_prime_UTR 30.0%
GTAAAAAGAAGAAGAAAGGG+AGG + chr8_3:5419284-5419303 None:intergenic 35.0%
!! ATGAAGAATGAGAAGTGTTG+TGG - chr8_3:5419549-5419568 Msa1271910:intron 35.0%
!! TGAAGAATGAGAAGTGTTGT+GGG - chr8_3:5419550-5419569 Msa1271910:intron 35.0%
TATGAGTGGAAGATATGAAG+GGG - chr8_3:5419593-5419612 Msa1271910:intron 35.0%
! GATGGGAAATATGAAGGTTT+TGG - chr8_3:5419624-5419643 Msa1271910:intron 35.0%
AGTAAGGGTAGTAGGTATAA+GGG - chr8_3:5419660-5419679 Msa1271910:intron 35.0%
!! GAAATGGGTTTGGTGTTTAT+AGG - chr8_3:5419703-5419722 Msa1271910:intron 35.0%
GTGTTCATACTTGTGATGAT+GGG - chr8_3:5419784-5419803 Msa1271910:intron 35.0%
!! GATTTGTTGGTGAGTTTAAG+TGG - chr8_3:5419811-5419830 Msa1271910:intron 35.0%
! TTTGTTGGTGAGTTTAAGTG+GGG - chr8_3:5419813-5419832 Msa1271910:intron 35.0%
! TTTAAGTGGGGTGTTAAACA+TGG - chr8_3:5419825-5419844 Msa1271910:intron 35.0%
AAACTCACCTAAAGTGATAG+TGG + chr8_3:5419857-5419876 None:intergenic 35.0%
AAATTACTAATGGTGTAGCC+GGG - chr8_3:5420015-5420034 Msa1271910:intron 35.0%
CAATCACAATTCAAGAACAC+CGG + chr8_3:5420053-5420072 None:intergenic 35.0%
CTACTGATATCAACAAACAC+AGG + chr8_3:5420190-5420209 None:intergenic 35.0%
! TATCAGTAGTTGGTTCAGTT+CGG - chr8_3:5420201-5420220 Msa1271910:intron 35.0%
CAGATGGAATTACTAAACTG+CGG - chr8_3:5420462-5420481 Msa1271910:CDS 35.0%
TGTTGAAAAACTGAGCTTGT+TGG - chr8_3:5420897-5420916 Msa1271910:intron 35.0%
! GGCATGCTTTTAATGAATAG+TGG - chr8_3:5420944-5420963 Msa1271910:intron 35.0%
ATATAGGTGCATCTCTTCTA+AGG - chr8_3:5421012-5421031 Msa1271910:intron 35.0%
ACTATGGAGCAAATAAAAGG+AGG + chr8_3:5421089-5421108 None:intergenic 35.0%
TATATTGCAGACAAGATGCA+TGG - chr8_3:5421183-5421202 Msa1271910:intron 35.0%
AGAAATGGAGAAACTCAATC+TGG - chr8_3:5421294-5421313 Msa1271910:intron 35.0%
! TAGACTGTATGGGTTTTTGT+TGG - chr8_3:5421873-5421892 Msa1271910:CDS 35.0%
TGTTGGAAAGAAAAAGTGGT+AGG - chr8_3:5421890-5421909 Msa1271910:CDS 35.0%
CGAGATTATGGACAGATAAT+TGG - chr8_3:5422002-5422021 Msa1271910:CDS 35.0%
! TGAGCTTTTTGTGAACAAAC+AGG - chr8_3:5422143-5422162 Msa1271910:CDS 35.0%
ATTTACTCGTTCATCTACCT+TGG + chr8_3:5422205-5422224 None:intergenic 35.0%
AATAGGGTTAGGGATTTGGA+GGG + chr8_3:5419123-5419142 None:intergenic 40.0%
GAAATTGGGGAATAGGGTTA+GGG + chr8_3:5419133-5419152 None:intergenic 40.0%
TGAAATTGGGGAATAGGGTT+AGG + chr8_3:5419134-5419153 None:intergenic 40.0%
AGAAGAAAGAGTGAGTAGGT+GGG + chr8_3:5419311-5419330 None:intergenic 40.0%
AAGAAGAAAGAGTGAGTAGG+TGG + chr8_3:5419312-5419331 None:intergenic 40.0%
GGAAAGACAGAAGAAAAGTG+CGG + chr8_3:5419364-5419383 None:intergenic 40.0%
CGGTGGTGATTTGAATTTGA+GGG + chr8_3:5419385-5419404 None:intergenic 40.0%
ACGGTGGTGATTTGAATTTG+AGG + chr8_3:5419386-5419405 None:intergenic 40.0%
TGTTTCACTGTGAGGACTAT+CGG + chr8_3:5419417-5419436 None:intergenic 40.0%
AGGGTTAGGGTTTATGCAAA+TGG - chr8_3:5419507-5419526 Msa1271910:intron 40.0%
! GCAAATGGTGATGTGTATGA+AGG - chr8_3:5419522-5419541 Msa1271910:intron 40.0%
TGGAAGATATGAAGGGGATT+GGG - chr8_3:5419599-5419618 Msa1271910:intron 40.0%
!! TGGGTTGATGGGAAATATGA+AGG - chr8_3:5419618-5419637 Msa1271910:intron 40.0%
!!! AAGGTTTTGGTGTTGAGAGT+TGG - chr8_3:5419637-5419656 Msa1271910:intron 40.0%
GAGTAAGGGTAGTAGGTATA+AGG - chr8_3:5419659-5419678 Msa1271910:intron 40.0%
!! GATGGATTGAGAAATGGGTT+TGG - chr8_3:5419693-5419712 Msa1271910:intron 40.0%
TATACTGGGGATGTTTATGC+TGG - chr8_3:5419729-5419748 Msa1271910:intron 40.0%
TATGCTGGTGAATGGTTGAA+TGG - chr8_3:5419744-5419763 Msa1271910:intron 40.0%
GGTGTTCATACTTGTGATGA+TGG - chr8_3:5419783-5419802 Msa1271910:intron 40.0%
!! GATGATGGGAGTAGATTTGT+TGG - chr8_3:5419798-5419817 Msa1271910:intron 40.0%
! ATCAGTAGTTGGTTCAGTTC+GGG - chr8_3:5420202-5420221 Msa1271910:intron 40.0%
TTCAGTTCGGGCCAAATATT+AGG - chr8_3:5420214-5420233 Msa1271910:intron 40.0%
! TCGTTACTTTATGTATCCGG+TGG - chr8_3:5420551-5420570 Msa1271910:intron 40.0%
GATGCTGGGTTTAGATTCAA+TGG - chr8_3:5420578-5420597 Msa1271910:intron 40.0%
TGCTTTATGCCTCTTCAAAC+TGG - chr8_3:5420601-5420620 Msa1271910:intron 40.0%
TCTCTTAAGTTCCTTGCAAG+AGG - chr8_3:5420641-5420660 Msa1271910:intron 40.0%
AGGTTACAAGTTCACAGTCA+TGG + chr8_3:5420673-5420692 None:intergenic 40.0%
ATGCAGAACAGAACAACAGT+TGG + chr8_3:5420717-5420736 None:intergenic 40.0%
CAACACTGATACACCCTATT+CGG - chr8_3:5420869-5420888 Msa1271910:intron 40.0%
AGTATCAGTGACTAGGATTC+TGG - chr8_3:5420922-5420941 Msa1271910:intron 40.0%
GTATCAGTGACTAGGATTCT+GGG - chr8_3:5420923-5420942 Msa1271910:intron 40.0%
AGAAATGGAGATACGTATGC+TGG - chr8_3:5421156-5421175 Msa1271910:intron 40.0%
CAGACAAGATGCATGGATTT+GGG - chr8_3:5421190-5421209 Msa1271910:intron 40.0%
AGACAAGATGCATGGATTTG+GGG - chr8_3:5421191-5421210 Msa1271910:intron 40.0%
! GGGGTATACTGTTTTGCAAA+TGG - chr8_3:5421210-5421229 Msa1271910:intron 40.0%
CAATCTGGTCACTGGAAAAA+TGG - chr8_3:5421309-5421328 Msa1271910:intron 40.0%
CTGCACTGTATTAAGTACTC+GGG + chr8_3:5421400-5421419 None:intergenic 40.0%
CCTGCACTGTATTAAGTACT+CGG + chr8_3:5421401-5421420 None:intergenic 40.0%
TATTCTTCACTGAAACCACG+AGG - chr8_3:5421785-5421804 Msa1271910:CDS 40.0%
ATTCTTCACTGAAACCACGA+GGG - chr8_3:5421786-5421805 Msa1271910:CDS 40.0%
AGACTGTTCAGTCAAAACTC+TGG + chr8_3:5421849-5421868 None:intergenic 40.0%
ACAGTCTAGACTAGACTGTA+TGG - chr8_3:5421862-5421881 Msa1271910:CDS 40.0%
CAGTCTAGACTAGACTGTAT+GGG - chr8_3:5421863-5421882 Msa1271910:CDS 40.0%
!! ATGGCATGCGTATTGACTAT+TGG + chr8_3:5421930-5421949 None:intergenic 40.0%
ATCTACCTTGGCTACATCAT+AGG + chr8_3:5422193-5422212 None:intergenic 40.0%
! TTCCGAATTCCTGTTTTGTG+AGG - chr8_3:5422324-5422343 Msa1271910:CDS 40.0%
TGCCTCACAAAACAGGAATT+CGG + chr8_3:5422329-5422348 None:intergenic 40.0%
AGTTGATTGCCTCACAAAAC+AGG + chr8_3:5422336-5422355 None:intergenic 40.0%
! AGTAGAGAAGCATGAGATTG+TGG - chr8_3:5422370-5422389 Msa1271910:CDS 40.0%
AGATTTGCAGCTAGCTATTC+TGG - chr8_3:5422396-5422415 Msa1271910:CDS 40.0%
! TATGGGTCTTGTATAGAGAG+AGG - chr8_3:5422485-5422504 Msa1271910:CDS 40.0%
! ATGGGTCTTGTATAGAGAGA+GGG - chr8_3:5422486-5422505 Msa1271910:CDS 40.0%
TTACAACAGTAGCTGGGTTA+AGG + chr8_3:5422638-5422657 None:intergenic 40.0%
GAATAGGGTTAGGGATTTGG+AGG + chr8_3:5419124-5419143 None:intergenic 45.0%
GAAGGAGGATTAGACGGAAA+CGG + chr8_3:5419257-5419276 None:intergenic 45.0%
GGGAAAGAAGGAGGATTAGA+CGG + chr8_3:5419263-5419282 None:intergenic 45.0%
AGAAGAAAGGGAGGGAAAGA+AGG + chr8_3:5419275-5419294 None:intergenic 45.0%
GAAGAAAGAGTGAGTAGGTG+GGG + chr8_3:5419310-5419329 None:intergenic 45.0%
! AAGACAGAAGAAAAGTGCGG+CGG + chr8_3:5419361-5419380 None:intergenic 45.0%
TAGTCCTCACAGTGAAACAG+TGG - chr8_3:5419418-5419437 Msa1271910:CDS 45.0%
GATTCCACTGTTTCACTGTG+AGG + chr8_3:5419425-5419444 None:intergenic 45.0%
CAGTGAAACAGTGGAATCGA+CGG - chr8_3:5419427-5419446 Msa1271910:CDS 45.0%
!! AATGAGAAGTGTTGTGGGAG+TGG - chr8_3:5419555-5419574 Msa1271910:intron 45.0%
!! ATGAGAAGTGTTGTGGGAGT+GGG - chr8_3:5419556-5419575 Msa1271910:intron 45.0%
GTGGAAGATATGAAGGGGAT+TGG - chr8_3:5419598-5419617 Msa1271910:intron 45.0%
!! TATGAAGGGGATTGGGTTGA+TGG - chr8_3:5419606-5419625 Msa1271910:intron 45.0%
!! ATGAAGGGGATTGGGTTGAT+GGG - chr8_3:5419607-5419626 Msa1271910:intron 45.0%
! TGGTGTTGAGAGTTGGAGTA+AGG - chr8_3:5419644-5419663 Msa1271910:intron 45.0%
! GGTGTTGAGAGTTGGAGTAA+GGG - chr8_3:5419645-5419664 Msa1271910:intron 45.0%
AGAGTTGGAGTAAGGGTAGT+AGG - chr8_3:5419652-5419671 Msa1271910:intron 45.0%
GGGATGTTTATGCTGGTGAA+TGG - chr8_3:5419736-5419755 Msa1271910:intron 45.0%
TGGTTGAATGGTCAGAGTCA+TGG - chr8_3:5419756-5419775 Msa1271910:intron 45.0%
GGTTGAATGGTCAGAGTCAT+GGG - chr8_3:5419757-5419776 Msa1271910:intron 45.0%
! TGGGGTGTTAAACATGGTCT+TGG - chr8_3:5419831-5419850 Msa1271910:intron 45.0%
GTCTTGGCCACTATCACTTT+AGG - chr8_3:5419847-5419866 Msa1271910:intron 45.0%
CAATTCAAGAACACCGGACA+TGG + chr8_3:5420047-5420066 None:intergenic 45.0%
AGTTCGGGCCAAATATTAGG+TGG - chr8_3:5420217-5420236 Msa1271910:intron 45.0%
ATGTTTCACCAGTTCCTAGC+TGG - chr8_3:5420380-5420399 Msa1271910:CDS 45.0%
GCTTTCAGACCAGTTTGAAG+AGG + chr8_3:5420613-5420632 None:intergenic 45.0%
CATGGACTTAACCTCTTGCA+AGG + chr8_3:5420655-5420674 None:intergenic 45.0%
! AGTGTTGCTGATGGTCAACA+AGG + chr8_3:5420856-5420875 None:intergenic 45.0%
GTTGGTCAGTATCAGTGACT+AGG - chr8_3:5420915-5420934 Msa1271910:intron 45.0%
GCAGACAAGATGCATGGATT+TGG - chr8_3:5421189-5421208 Msa1271910:intron 45.0%
GCAAATGGCCATCGTTATGA+AGG - chr8_3:5421225-5421244 Msa1271910:intron 45.0%
TTATGAAGGAACCTGGCATG+AGG - chr8_3:5421239-5421258 Msa1271910:intron 45.0%
TATGAAGGAACCTGGCATGA+GGG - chr8_3:5421240-5421259 Msa1271910:intron 45.0%
GAGAAACTCAATCTGGTCAC+TGG - chr8_3:5421301-5421320 Msa1271910:intron 45.0%
AACAGGGGAAACTGGATATG+TGG + chr8_3:5421364-5421383 None:intergenic 45.0%
ACATATCCAGTTTCCCCTGT+TGG - chr8_3:5421363-5421382 Msa1271910:intron 45.0%
CCGAGTACTTAATACAGTGC+AGG - chr8_3:5421398-5421417 Msa1271910:intron 45.0%
TTCTTCACTGAAACCACGAG+GGG - chr8_3:5421787-5421806 Msa1271910:CDS 45.0%
GAAAGCCTATGATGTAGCCA+AGG - chr8_3:5422185-5422204 Msa1271910:CDS 45.0%
TTTGCAGCTAGCTATTCTGG+TGG - chr8_3:5422399-5422418 Msa1271910:CDS 45.0%
GTCTTGTATAGAGAGAGGGA+AGG - chr8_3:5422490-5422509 Msa1271910:CDS 45.0%
TCATGTAGAGTCACGTATGC+TGG - chr8_3:5422599-5422618 Msa1271910:CDS 45.0%
GGGATCTTACAACAGTAGCT+GGG + chr8_3:5422644-5422663 None:intergenic 45.0%
TGGGATCTTACAACAGTAGC+TGG + chr8_3:5422645-5422664 None:intergenic 45.0%
TCAAAACATCTTGCGCACGA+TGG + chr8_3:5422688-5422707 None:intergenic 45.0%
!!! TTTTTTTTTTAAATTACTAA+TGG - chr8_3:5420005-5420024 Msa1271910:intron 5.0%
GGGGAATAGGGTTAGGGATT+TGG + chr8_3:5419127-5419146 None:intergenic 50.0%
AGAAAGGGAGGGAAAGAAGG+AGG + chr8_3:5419272-5419291 None:intergenic 50.0%
CGGAGAAAGCGAAAGCAGAT+AGG + chr8_3:5419341-5419360 None:intergenic 50.0%
AGGACTATCGGAAGAGACGA+CGG + chr8_3:5419405-5419424 None:intergenic 50.0%
TGAAACAGTGGAATCGACGG+CGG - chr8_3:5419430-5419449 Msa1271910:CDS 50.0%
!! TGAGAAGTGTTGTGGGAGTG+GGG - chr8_3:5419557-5419576 Msa1271910:intron 50.0%
AATGGTCAGAGTCATGGGAG+TGG - chr8_3:5419762-5419781 Msa1271910:intron 50.0%
AGCGGGGTTTCGACACATTT+AGG - chr8_3:5420405-5420424 Msa1271910:CDS 50.0%
GCGGGGTTTCGACACATTTA+GGG - chr8_3:5420406-5420425 Msa1271910:CDS 50.0%
!! TATCCGGTGGTTCTGATGCT+GGG - chr8_3:5420564-5420583 Msa1271910:intron 50.0%
AAACCCAGCATCAGAACCAC+CGG + chr8_3:5420570-5420589 None:intergenic 50.0%
GTTGTTGCTACGTGATCTGG+AGG + chr8_3:5420693-5420712 None:intergenic 50.0%
GGAGTTGTTGCTACGTGATC+TGG + chr8_3:5420696-5420715 None:intergenic 50.0%
! GGGTGTATCAGTGTTGCTGA+TGG + chr8_3:5420865-5420884 None:intergenic 50.0%
GCCATCGTTATGAAGGAACC+TGG - chr8_3:5421232-5421251 Msa1271910:intron 50.0%
GCCAGGTTCCTTCATAACGA+TGG + chr8_3:5421236-5421255 None:intergenic 50.0%
ATGAAGGAACCTGGCATGAG+GGG - chr8_3:5421241-5421260 Msa1271910:intron 50.0%
! ATGTCTTTTCCCCTCATGCC+AGG + chr8_3:5421253-5421272 None:intergenic 50.0%
TGGCATGAGGGGAAAAGACA+TGG - chr8_3:5421252-5421271 Msa1271910:intron 50.0%
GAGGGGAAAAGACATGGTCT+TGG - chr8_3:5421258-5421277 Msa1271910:intron 50.0%
TTGACACCAACAGGGGAAAC+TGG + chr8_3:5421372-5421391 None:intergenic 50.0%
GGAGTGATTGACACCAACAG+GGG + chr8_3:5421379-5421398 None:intergenic 50.0%
GGGAGTGATTGACACCAACA+GGG + chr8_3:5421380-5421399 None:intergenic 50.0%
TCTTCACTGAAACCACGAGG+GGG - chr8_3:5421788-5421807 Msa1271910:CDS 50.0%
AGCAGCTACTCTAGCTGCAT+TGG + chr8_3:5422256-5422275 None:intergenic 50.0%
AGCTAGAGTAGCTGCTGTCA+AGG - chr8_3:5422260-5422279 Msa1271910:CDS 50.0%
TCAGCAACTGGTGGAGATGA+TGG + chr8_3:5422434-5422453 None:intergenic 50.0%
! AGTTGCTGACTGAACGCAAG+CGG - chr8_3:5422445-5422464 Msa1271910:CDS 50.0%
GGATTAGACGGAAACGGTGG+CGG + chr8_3:5419251-5419270 None:intergenic 55.0%
GGAGGATTAGACGGAAACGG+TGG + chr8_3:5419254-5419273 None:intergenic 55.0%
ACTATCGGAAGAGACGACGG+TGG + chr8_3:5419402-5419421 None:intergenic 55.0%
ACCAGTTCCTAGCTGGCTAG+CGG - chr8_3:5420387-5420406 Msa1271910:CDS 55.0%
!! GTATCCGGTGGTTCTGATGC+TGG - chr8_3:5420563-5420582 Msa1271910:intron 55.0%
TATGTGGCGTTCTGCGTGCT+TGG + chr8_3:5421348-5421367 None:intergenic 55.0%
CGGGAGTGATTGACACCAAC+AGG + chr8_3:5421381-5421400 None:intergenic 55.0%
CTTCACTGAAACCACGAGGG+GGG - chr8_3:5421789-5421808 Msa1271910:CDS 55.0%
ATTGTTCTGTTCCCCCCTCG+TGG + chr8_3:5421803-5421822 None:intergenic 55.0%
TGCGTTCAGTCAGCAACTGG+TGG + chr8_3:5422443-5422462 None:intergenic 55.0%
GCTTGCGTTCAGTCAGCAAC+TGG + chr8_3:5422446-5422465 None:intergenic 55.0%
AGTCACGTATGCTGGTGTGC+TGG - chr8_3:5422607-5422626 Msa1271910:CDS 55.0%
CCAGTTCCTAGCTGGCTAGC+GGG - chr8_3:5420388-5420407 Msa1271910:CDS 60.0%
CAGTTCCTAGCTGGCTAGCG+GGG - chr8_3:5420389-5420408 Msa1271910:CDS 60.0%
GAGTGCGGCGGATGAGATGG+CGG + chr8_3:5419220-5419239 None:intergenic 65.0%
!! GACGGAAACGGTGGCGGAGA+CGG + chr8_3:5419245-5419264 None:intergenic 65.0%
AGCCGGGTCAGTGCCATGTC+CGG - chr8_3:5420031-5420050 Msa1271910:intron 65.0%
!! CACCGGACATGGCACTGACC+CGG + chr8_3:5420036-5420055 None:intergenic 65.0%
CCCGCTAGCCAGCTAGGAAC+TGG + chr8_3:5420391-5420410 None:intergenic 65.0%
CGAAACCCCGCTAGCCAGCT+AGG + chr8_3:5420397-5420416 None:intergenic 65.0%
GCGGAGTGCGGCGGATGAGA+TGG + chr8_3:5419223-5419242 None:intergenic 70.0%
!! GGAAACGGTGGCGGAGACGG+CGG + chr8_3:5419242-5419261 None:intergenic 70.0%
! GTGGCGGAGACGGCGGAGTG+CGG + chr8_3:5419235-5419254 None:intergenic 75.0%
! GCGGAGACGGCGGAGTGCGG+CGG + chr8_3:5419232-5419251 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 5418869 5422775 5418869 ID=Msa1271910;Name=Msa1271910
chr8_3 mRNA 5418869 5422775 5418869 ID=Msa1271910-mRNA-1;Parent=Msa1271910;Name=Msa1271910-mRNA-1;_AED=0.02;_eAED=0.02;_QI=86|1|1|1|1|1|3|431|451
chr8_3 exon 5418869 5419479 5418869 ID=Msa1271910-mRNA-1:exon:1971;Parent=Msa1271910-mRNA-1
chr8_3 exon 5420225 5420486 5420225 ID=Msa1271910-mRNA-1:exon:1970;Parent=Msa1271910-mRNA-1
chr8_3 exon 5421776 5422775 5421776 ID=Msa1271910-mRNA-1:exon:1969;Parent=Msa1271910-mRNA-1
chr8_3 five_prime_UTR 5422690 5422775 5422690 ID=Msa1271910-mRNA-1:five_prime_utr;Parent=Msa1271910-mRNA-1
chr8_3 CDS 5421776 5422689 5421776 ID=Msa1271910-mRNA-1:cds;Parent=Msa1271910-mRNA-1
chr8_3 CDS 5420225 5420486 5420225 ID=Msa1271910-mRNA-1:cds;Parent=Msa1271910-mRNA-1
chr8_3 CDS 5419300 5419479 5419300 ID=Msa1271910-mRNA-1:cds;Parent=Msa1271910-mRNA-1
chr8_3 three_prime_UTR 5418869 5419299 5418869 ID=Msa1271910-mRNA-1:three_prime_utr;Parent=Msa1271910-mRNA-1
Gene Sequence

>Msa1271910

ATGCATCAGAAAAAATCAGAAATTCAGATCGGAAAAGAAAGTTCTTGTATCTCTTCCGATTTCAACCCTAATCTCTTTCATCCTCATAAACTTTTCAATTCAATTCACCACAAAAACCAACACAATTCTATTTCTTCACCAATTCATCATAAAATCAAAATCAAACCCTCCAAATCCCTAACCCTATTCCCCAATTTCAATTTCAATTTCAATTTCCCACAATTTTCCACTAAAAAATCATCTTTTCATTTTCATTCTCTCTCCGCCATCTCATCCGCCGCACTCCGCCGTCTCCGCCACCGTTTCCGTCTAATCCTCCTTCTTTCCCTCCCTTTCTTCTTCTTTTTACTATCCCCACCTACTCACTCTTTCTTCTTTAATTTCCTATCTGCTTTCGCTTTCTCCGCCGCACTTTTCTTCTGTCTTTCCCTCAAATTCAAATCACCACCGTCGTCTCTTCCGATAGTCCTCACAGTGAAACAGTGGAATCGACGGCGGAGAGAGAATAAAATTTCCAAAAATTCGAAAAATTCATCCAATATTGTGGAAATTAGGGTTAGGGTTTATGCAAATGGTGATGTGTATGAAGGTGAAATGAAGAATGAGAAGTGTTGTGGGAGTGGGGTTTATTACTATAATATGAGTGGAAGATATGAAGGGGATTGGGTTGATGGGAAATATGAAGGTTTTGGTGTTGAGAGTTGGAGTAAGGGTAGTAGGTATAAGGGTATGTATAGAGATGGATTGAGAAATGGGTTTGGTGTTTATAGGTTTTATACTGGGGATGTTTATGCTGGTGAATGGTTGAATGGTCAGAGTCATGGGAGTGGTGTTCATACTTGTGATGATGGGAGTAGATTTGTTGGTGAGTTTAAGTGGGGTGTTAAACATGGTCTTGGCCACTATCACTTTAGAAATGGAGATACGTATGCTGGTGAGTATATTGCAGACAAGATGCATGGATTTGGGGTATACTGTTTTGCAAATGGCCATCGTTATGAAGGAACCTGGCATGAGGGGAAAAGACATGGTCTTGGAATGTATACATTTAGAAATGGAGAAACTCAATCTGGTCACTGGAAAAATGGAGTACTTGACATTCCAAGCACGCAGAACGCCACATATCCAGTTTCCCCTGTTGGTGTCAATCACTCCCGAGTACTTAATACAGTGCAGGAAGCTAGAAGAGCAGCTGAGAAAGCCTATGATGTAGCCAAGGTAGATGAACGAGTAAATAGAGCAGTATCAGCAGCTAATAGAGCAGCCAATGCAGCTAGAGTAGCTGCTGTCAAGGCTGTGCAAAATGAAATGCATCATCATGTTAACGAAGAGAGCTTCCGAATTCCTGTTTTGTGA
Protein sequence

>Msa1271910

MHQKKSEIQIGKESSCISSDFNPNLFHPHKLFNSIHHKNQHNSISSPIHHKIKIKPSKSLTLFPNFNFNFNFPQFSTKKSSFHFHSLSAISSAALRRLRHRFRLILLLSLPFFFFLLSPPTHSFFFNFLSAFAFSAALFFCLSLKFKSPPSSLPIVLTVKQWNRRRRENKISKNSKNSSNIVEIRVRVYANGDVYEGEMKNEKCCGSGVYYYNMSGRYEGDWVDGKYEGFGVESWSKGSRYKGMYRDGLRNGFGVYRFYTGDVYAGEWLNGQSHGSGVHTCDDGSRFVGEFKWGVKHGLGHYHFRNGDTYAGEYIADKMHGFGVYCFANGHRYEGTWHEGKRHGLGMYTFRNGETQSGHWKNGVLDIPSTQNATYPVSPVGVNHSRVLNTVQEARRAAEKAYDVAKVDERVNRAVSAANRAANAARVAAVKAVQNEMHHHVNEESFRIPVL*