AlfalfaGEDB | Msa1284760

M. sativa cultivar zhongmu-4 / Msa1284760

KEGG: -
GO: GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF01453.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1284760	sp\|Q9LW83\|CE101_ARATH	28.257	499	254	20	333	752	70	543	1.06e-32	139
Msa1284760	sp\|Q9LW83\|CE101_ARATH	38.418	177	94	5	68	231	70	244	9.11e-22	104

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1284760	G7LA16	63.343	341	94	8	40	369	354	674	4.31e-125	399
Msa1284760	G7LA16	65.402	659	126	16	1	585	1	631	0.0	798
Msa1284760	G7LA16	73.134	335	67	7	300	626	36	355	1.91e-158	485

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017510	Msa1284760	0.840248	9.749061e-58	-8.615850e-47
Msa0028640	Msa1284760	0.805858	1.089537e-49	-8.615850e-47
Msa0110970	Msa1284760	0.825635	4.210410e-54	-8.615850e-47
Msa0140780	Msa1284760	0.816676	4.890595e-52	-8.615850e-47
Msa0157290	Msa1284760	0.839797	1.277741e-57	-8.615850e-47
Msa0200260	Msa1284760	0.802290	6.018186e-49	-8.615850e-47
Msa0243100	Msa1284760	0.821673	3.562979e-53	-8.615850e-47
Msa0287650	Msa1284760	0.813743	2.193536e-51	-8.615850e-47
Msa0341540	Msa1284760	0.802678	5.005649e-49	-8.615850e-47
Msa0392510	Msa1284760	0.809436	1.894103e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1284760	MtrunA17_Chr8g0357931	77.996	459	84	4	300	752	36	483	0.0	707
Msa1284760	MtrunA17_Chr8g0357931	73.968	315	71	3	1	308	1	311	8.93e-161	479
Msa1284760	MtrunA17_Chr8g0358081	74.837	461	90	8	300	752	36	478	0.0	672
Msa1284760	MtrunA17_Chr8g0358081	68.085	329	83	6	1	321	1	315	9.22e-142	436
Msa1284760	MtrunA17_Chr8g0357961	71.064	470	115	8	300	752	36	501	0.0	668
Msa1284760	MtrunA17_Chr8g0357961	71.250	320	76	5	1	304	1	320	4.16e-155	471
Msa1284760	MtrunA17_Chr8g0358121	75.974	462	84	9	300	752	36	479	0.0	665
Msa1284760	MtrunA17_Chr8g0358121	70.978	317	69	7	1	308	1	303	1.99e-144	442
Msa1284760	MtrunA17_Chr8g0358031	71.581	468	110	9	300	752	37	496	0.0	658
Msa1284760	MtrunA17_Chr8g0358031	70.159	315	79	6	1	304	2	312	5.41e-142	437

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1284760	AT3G16030.1	28.257	499	254	20	333	752	70	543	1.07e-33	139
Msa1284760	AT3G16030.1	38.418	177	94	5	68	231	70	244	9.26e-23	104
Msa1284760	AT3G16030.3	27.490	502	262	19	334	752	82	564	1.91e-32	134
Msa1284760	AT3G16030.3	38.636	176	93	5	69	231	82	255	6.14e-23	104
Msa1284760	AT3G16030.8	27.490	502	262	19	334	752	82	564	1.91e-32	134
Msa1284760	AT3G16030.8	38.636	176	93	5	69	231	82	255	6.14e-23	104
Msa1284760	AT3G16030.5	27.490	502	262	19	334	752	82	564	1.91e-32	134
Msa1284760	AT3G16030.5	38.636	176	93	5	69	231	82	255	6.14e-23	104
Msa1284760	AT3G16030.7	27.490	502	262	19	334	752	82	564	2.32e-32	134
Msa1284760	AT3G16030.7	38.636	176	93	5	69	231	82	255	7.05e-23	104

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Find 170 sgRNAs with CRISPR-Local

Find 935 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGAAGGGAGCAATGATT+TGG	0.435822	8_3:-27727619	Msa1284760:CDS
AAATCAGCTCGGAATTCCCA+TGG	0.641971	8_3:-27736390	Msa1284760:intron
AAATTCACCCGAACTCGGGA+GGG	0.630800	8_3:+27739564	None:intergenic
AACCAATGACCAATTATGTC+CGG	0.495276	8_3:+27737031	None:intergenic
AACCGGACATAATTGGTCAT+TGG	0.393190	8_3:-27737033	Msa1284760:CDS
AACTAATGACCAATTATGTC+CGG	0.455117	8_3:+27739603	None:intergenic
AACTTGCAGGGACCCTGGTG+AGG	0.662301	8_3:-27736643	Msa1284760:CDS
AAGAAAAGTAAAAGAAAGGA+AGG	0.407514	8_3:-27727633	Msa1284760:CDS
AAGATGGAAAGTTTATGCGA+TGG	0.607356	8_3:-27736788	Msa1284760:CDS
AAGATGGAAAGTTTATGCGA+TGG	0.607356	8_3:-27739360	Msa1284760:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTGCTGAATCTATGTCTAT+GGG	+	chr8_3:27727674-27727693	None:intergenic	30.0%
AATTGCTGAATCTATGTCTA+TGG	+	chr8_3:27727675-27727694	None:intergenic	30.0%
ATTCTATCACTAGATTACTC+TGG	-	chr8_3:27727692-27727711	Msa1284760:intron	30.0%
ATAAACTCTTTGTCCCATTT+GGG	+	chr8_3:27727841-27727860	None:intergenic	30.0%
AATAAACTCTTTGTCCCATT+TGG	+	chr8_3:27727842-27727861	None:intergenic	30.0%
ATGGGACAAAGAGTTTATTA+TGG	-	chr8_3:27727843-27727862	Msa1284760:CDS	30.0%
AACTAATGACCAATTATGTC+CGG	+	chr8_3:27727933-27727952	None:intergenic	30.0%
GAATTGAACATCAAGAAAAG+TGG	-	chr8_3:27728019-27728038	Msa1284760:intron	30.0%
AGTGGAAAACTTAAAAGCAA+TGG	-	chr8_3:27728058-27728077	Msa1284760:intron	30.0%
TAAAAGCAATGGAATATTCG+AGG	-	chr8_3:27728069-27728088	Msa1284760:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	27727434	27740121	27727434	ID=Msa1284760;Name=Msa1284760
chr8_3	mRNA	27727434	27740121	27727434	ID=Msa1284760-mRNA-1;Parent=Msa1284760;Name=Msa1284760-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0\|0.33\|0\|1\|0.66\|0.5\|4\|0\|764
chr8_3	exon	27739221	27740121	27739221	ID=Msa1284760-mRNA-1:exon:9442;Parent=Msa1284760-mRNA-1
chr8_3	exon	27736391	27737443	27736391	ID=Msa1284760-mRNA-1:exon:9441;Parent=Msa1284760-mRNA-1
chr8_3	exon	27727749	27727865	27727749	ID=Msa1284760-mRNA-1:exon:9440;Parent=Msa1284760-mRNA-1
chr8_3	exon	27727434	27727657	27727434	ID=Msa1284760-mRNA-1:exon:9439;Parent=Msa1284760-mRNA-1
chr8_3	CDS	27739221	27740121	27739221	ID=Msa1284760-mRNA-1:cds;Parent=Msa1284760-mRNA-1
chr8_3	CDS	27736391	27737443	27736391	ID=Msa1284760-mRNA-1:cds;Parent=Msa1284760-mRNA-1
chr8_3	CDS	27727749	27727865	27727749	ID=Msa1284760-mRNA-1:cds;Parent=Msa1284760-mRNA-1
chr8_3	CDS	27727434	27727657	27727434	ID=Msa1284760-mRNA-1:cds;Parent=Msa1284760-mRNA-1

Gene Sequence

>Msa1284760

ATGATAAGCTTTAAAATAAAGAAACAAATTGTTTTGATATACTTATGGTTGTGGTGGAGTACTACTAGTATTTGTGTTAATGCAACAAGTGATAGTTTGAAGCCAGGTGATACATTGAATTCCAATTCCTCGCTATGTTCTAAACAAGGCAAGTTTTGTCTTCTATTCGGCAAAATTGGTCACTTGATTATAAGTGCTAATATCAATACAGCACTGTGGATGTATGACAGAAATGAACCCATAGACATAGATTCAGCAATTCTATCACTAGATTACTCTGGTGTGCTCAAAATAGAATTTCAAAATAGGAATTTACCAATAATCATTTACTCTTCGCCTCAACCCACCAACAACACTGTCGCCACTATGTTAGACAACGGTAACTTTGTTCTTCAACAACTTCACCCAAATGGGACAAAGAGTTTATTATGGCAGAGTTTTGATTATCCAACATACATTTTGATCCCAACGATGAAGTTGGGAGTTAATCGAAAAACCGGACATAATTGGTCATTAGTTTCAAAGTTTACTCATTCCCTCCCGAGTTCGGGTGAATTTAGTCTTGAATGGGAACCGAAACAAGGTGAATTGAACATCAAGAAAAGTGGCAAAGTGTATTGGAAAAGTGGAAAACTTAAAAGCAATGGAATATTCGAGGACATTCCGGCGAATGTTCAACATATGTATCAATACATTATTGTCTCTAACAAGATTGAAGAGTCTTTCACTTTCAAAGTTAAAGATGGAAAGTTTATGCGATGGTACCTAACTGAGGAGGGGAGGTTAGAAGGTGGTAATGGACACAATGACGTTGATATTGGAAATGCAGACATTTGTTATGGATATAATAGTGATGGAGGGTGCCAAAAATGGGAGGATATACCAACTTGCAGGGACCCTGGTGATACATTGAATTCCACTTCCTCGCTATGTTCTAAACAAGGCAAGTTTTGTCTTGAATTCGGCAAAATTGGTCACTTGATTATAAGTGCTAATATCAATACACCACTGTGGATGTATGACAGAAATCAACCCATGGACATAGATTCAGCAATTCTATCACTAGATTACTCTGGTAGGCTCAAAATAGAATTTCAAAATAGGAATTTACCAATAATCATTTACTCTTCGTCTCAACCCACCAACAACACTGTCGCAACTATGTTGGACACTGGTAACTTTGTGCTTCAACAACTTCACCCAAATGGGACAAAGAGTATATTATGGCAGAGTTTTGATTATCCCATAGATGTTTTGATCCCAACGATGAAGTTGGGAGTTAATCGAAAAACCGGACATAATTGGTCATTGGTTTCAGATTTGACTCCTTTGCTTATAAATTCAGGTGAGTTTAGTCTTGAATGGGAACCAAAACAAGGTGAATTGAACATCAAGAAAAGTGGCAAAGTGTATTGGAAAAGTGGAAAACTTAAAAGCAATGGAATATTCGAGGACATTCCGGCGAATGTTCAACATATGTATCAATACATTATTGTCTCTAACAAGACTGAAGAGTCTTTCACTTTCAAAGTTAAAGATGGAAAGTTTATGCGATGGTACCTAACTGAGGAGGGGAGGTTAGAAGGTGGTGATGGACACAATGACGTTGATATTGGAAATGCAGACATTTGTTATGGATATAATAGTGATGGAGGGTGCCAAAAATGGGAGGATATACCAACTTGCAGGGACCCTGGTGAGGTGTTTCAGGAAAAGGCAGGAAGACCAAACATAGACAATTCAACGTGGTGGTTTGAACTGGATGTGACTTATAGTTACAGTGATTGCAAGGTGAGATGTTGGAAAAATTGTAGTTGCAATGGATTCCAAGAATTTTATCCTAATGGGACTGAATGTATATTCTATTCATGGAATTCGACACAAGATGTTGAGGTGGTTTCGAATACCTTTTACACCTTGGTGAAGCCAACAAAATCAGCTCGGAATTCCCATGTCCATGCAGGTGTAAAAAGGTGGATATGGATAGGTTCTGCGATCGCAATTGCTTTACTCATATTATGCCCACTCATTATATGCCTAGCCAAAAAGAAACAAAAATATGCACTACAAGATAAGAAAAGTAAAAGAAAGGAAGGGAGCAATGATTTGGTGGAATCATATGATATCAAAGATCTTGAAGATGACTTCAAGGGACATGATATAAAAGTTTTTACCTTTACATCAATCTTGGAAGCAACTATGGAATTTTCCCCCGAAAACAAGTTAGGACAGGGAGGCTATGGACCCGTTTATAAGGTGACTGCAAAGTTAAATTGTATAATTATGTTTGTTTAA

Protein sequence

>Msa1284760

MISFKIKKQIVLIYLWLWWSTTSICVNATSDSLKPGDTLNSNSSLCSKQGKFCLLFGKIGHLIISANINTALWMYDRNEPIDIDSAILSLDYSGVLKIEFQNRNLPIIIYSSPQPTNNTVATMLDNGNFVLQQLHPNGTKSLLWQSFDYPTYILIPTMKLGVNRKTGHNWSLVSKFTHSLPSSGEFSLEWEPKQGELNIKKSGKVYWKSGKLKSNGIFEDIPANVQHMYQYIIVSNKIEESFTFKVKDGKFMRWYLTEEGRLEGGNGHNDVDIGNADICYGYNSDGGCQKWEDIPTCRDPGDTLNSTSSLCSKQGKFCLEFGKIGHLIISANINTPLWMYDRNQPMDIDSAILSLDYSGRLKIEFQNRNLPIIIYSSSQPTNNTVATMLDTGNFVLQQLHPNGTKSILWQSFDYPIDVLIPTMKLGVNRKTGHNWSLVSDLTPLLINSGEFSLEWEPKQGELNIKKSGKVYWKSGKLKSNGIFEDIPANVQHMYQYIIVSNKTEESFTFKVKDGKFMRWYLTEEGRLEGGDGHNDVDIGNADICYGYNSDGGCQKWEDIPTCRDPGEVFQEKAGRPNIDNSTWWFELDVTYSYSDCKVRCWKNCSCNGFQEFYPNGTECIFYSWNSTQDVEVVSNTFYTLVKPTKSARNSHVHAGVKRWIWIGSAIAIALLILCPLIICLAKKKQKYALQDKKSKRKEGSNDLVESYDIKDLEDDFKGHDIKVFTFTSILEATMEFSPENKLGQGGYGPVYKVTAKLNCIIMFV*