AlfalfaGEDB | Msa1356610

M. sativa cultivar zhongmu-4 / Msa1356610

KEGG: K03062
GO: GO:0000003 GO:0000502 GO:0002376 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0005886 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007276 GO:0007292 GO:0008134 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009628 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009933 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010074 GO:0010078 GO:0010101 GO:0010102 GO:0010243 GO:0010311 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019827 GO:0019941 GO:0019953 GO:0022414 GO:0022607 GO:0022622 GO:0022624 GO:0030162 GO:0030163 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033554 GO:0034622 GO:0034976 GO:0035266 GO:0035966 GO:0036402 GO:0036503 GO:0040007 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043248 GO:0043254 GO:0043632 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044703 GO:0045087 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0046686 GO:0048232 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048532 GO:0048589 GO:0048609 GO:0048646 GO:0048731 GO:0048827 GO:0048829 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051704 GO:0051716 GO:0051788 GO:0060255 GO:0060260 GO:0060261 GO:0060968 GO:0061136 GO:0065003 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090351 GO:0090696 GO:0090697 GO:0090698 GO:0098727 GO:0099402 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000112 GO:2000142 GO:2000144 GO:2001141
PFAM: PF16450.8

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1356610	XP_003625484.1	100.000	445	0	0	1	445	1	445	0.0	896

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1356610	sp\|Q9SZD4\|PRS4A_ARATH	95.730	445	17	2	1	445	1	443	0.0	854

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1356610	Q2HTA3	100.000	445	0	0	1	445	1	445	0.0	896

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000880	Msa1356610	0.814587	1.428353e-51	-8.615850e-47
Msa0000910	Msa1356610	0.823672	1.220890e-53	-8.615850e-47
Msa0001450	Msa1356610	0.849064	4.109043e-60	-8.615850e-47
Msa0002560	Msa1356610	0.844443	7.535622e-59	-8.615850e-47
Msa0002680	Msa1356610	0.827870	1.231992e-54	-8.615850e-47
Msa0003220	Msa1356610	0.892985	9.572178e-75	-8.615850e-47
Msa0003270	Msa1356610	0.861856	7.642441e-64	-8.615850e-47
Msa0003700	Msa1356610	0.810498	1.118590e-50	-8.615850e-47
Msa0003720	Msa1356610	0.827493	1.517575e-54	-8.615850e-47
Msa0004540	Msa1356610	0.854478	1.198166e-61	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1356610	MtrunA17_Chr7g0262801	100.000	445	0	0	1	445	1	445	0.0	896
Msa1356610	MtrunA17_Chr1g0182711	96.644	447	12	2	1	445	1	446	0.0	862
Msa1356610	MtrunA17_Chr4g0050561	48.556	381	185	4	65	437	30	407	2.43e-115	344
Msa1356610	MtrunA17_Chr1g0192151	45.669	381	185	2	77	436	37	416	4.33e-112	336
Msa1356610	MtrunA17_Chr4g0014731	44.737	380	189	1	78	436	38	417	8.75e-112	335
Msa1356610	MtrunA17_Chr3g0108321	52.288	306	141	3	67	370	40	342	1.01e-111	333
Msa1356610	MtrunA17_Chr3g0145011	46.092	371	193	2	73	436	18	388	2.74e-107	323
Msa1356610	MtrunA17_Chr3g0141901	45.251	358	196	0	79	436	28	385	2.91e-105	318
Msa1356610	MtrunA17_Chr8g0345461	46.334	341	175	3	111	445	85	423	1.27e-104	317
Msa1356610	MtrunA17_Chr1g0200081	49.201	313	154	3	135	445	123	432	2.18e-101	309

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1356610	AT4G29040.1	95.730	445	17	2	1	445	1	443	0.0	854
Msa1356610	AT2G20140.1	95.506	445	18	2	1	445	1	443	0.0	852
Msa1356610	AT5G58290.1	51.381	362	172	2	66	426	33	391	6.43e-133	389
Msa1356610	AT5G20000.1	52.000	325	155	1	114	437	85	409	2.49e-112	337
Msa1356610	AT5G19990.1	51.692	325	156	1	114	437	85	409	3.89e-112	337
Msa1356610	AT5G19990.3	51.692	325	156	1	114	437	85	409	3.89e-112	337
Msa1356610	AT5G19990.2	51.692	325	156	1	114	437	27	351	4.95e-112	334
Msa1356610	AT1G09100.1	45.526	380	186	2	78	436	37	416	1.68e-110	332
Msa1356610	AT3G05530.1	46.006	363	175	1	95	436	55	417	1.95e-110	332
Msa1356610	AT1G45000.1	46.361	371	192	3	73	436	15	385	8.75e-106	320

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Find 137 sgRNAs with CRISPR-Local

Find 377 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACACCGAAATGAAACAA+AGG	0.594716	tig0015812:+12999	Msa1356610:intergenic
AAAAGTTCCCTCACAAGTTT+TGG	0.248853	tig0015812:+15418	Msa1356610:intergenic
AAAATCCCAACATAATACTC+CGG	0.653134	tig0015812:+17655	Msa1356610:intergenic
AAAGCAATATGTACCGAAGC+TGG	0.494002	tig0015812:-14767	Msa1356610:CDS
AAAGGACAAGAAATTCGAAC+CGG	0.554284	tig0015812:-17983	Msa1356610:CDS
AAAGGAGGCAGGGGTAAATG+AGG	0.586817	tig0015812:+13017	Msa1356610:intergenic
AAATGAAACAAAGGAGGCAG+GGG	0.606531	tig0015812:+13008	Msa1356610:intergenic
AAATTTAGATACATACATCT+AGG	0.429419	tig0015812:-14862	Msa1356610:intron
AAGAAAGAAGGGGTGCCTGA+AGG	0.613773	tig0015812:-13061	Msa1356610:CDS
AAGGAATAAATAGGTATAGA+TGG	0.426273	tig0015812:+12751	Msa1356610:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATATTATAGGACGAGGAAT+TGG	+	tig0015812:12651-12670	Msa0956570:intergenic	30.0%
AAAATCCCAACATAATACTC+CGG	+	tig0015812:13135-13154	Msa1356610:intergenic	30.0%
TGAGAAAACATATAGTTGGA+AGG	-	tig0015812:13343-13362	Msa1356610:intron	30.0%
AGTATCAATAAAATGGTTGG+CGG	-	tig0015812:13830-13849	Msa1356610:intron	30.0%
TTTGTGTTCTATCTTCACTT+GGG	-	tig0015812:14028-14047	Msa1356610:intron	30.0%
GGAATCATACTAAATATGAG+GGG	+	tig0015812:14134-14153	Msa1356610:intergenic	30.0%
CGGAATCATACTAAATATGA+GGG	+	tig0015812:14135-14154	Msa1356610:intergenic	30.0%
ACGGAATCATACTAAATATG+AGG	+	tig0015812:14136-14155	Msa1356610:intergenic	30.0%
ATGATTCCGTGACTTTATTA+TGG	-	tig0015812:14146-14165	Msa1356610:intron	30.0%
TTCACACCATAATAAAGTCA+CGG	+	tig0015812:14155-14174	Msa1356610:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0015812	gene	12644	18165	12644	ID=Msa1356610;Name=Msa1356610
tig0015812	mRNA	12644	18165	12644	ID=Msa1356610-mRNA-1;Parent=Msa1356610;Name=Msa1356610-mRNA-1;_AED=0.07;_eAED=0.07;_QI=89\|1\|1\|1\|1\|1\|6\|404\|445
tig0015812	exon	12644	13131	12644	ID=Msa1356610-mRNA-1:exon:10054;Parent=Msa1356610-mRNA-1
tig0015812	exon	14736	14876	14736	ID=Msa1356610-mRNA-1:exon:10053;Parent=Msa1356610-mRNA-1
tig0015812	exon	14977	15193	14977	ID=Msa1356610-mRNA-1:exon:10052;Parent=Msa1356610-mRNA-1
tig0015812	exon	15349	15518	15349	ID=Msa1356610-mRNA-1:exon:10051;Parent=Msa1356610-mRNA-1
tig0015812	exon	16327	16572	16327	ID=Msa1356610-mRNA-1:exon:10050;Parent=Msa1356610-mRNA-1
tig0015812	exon	17597	18165	17597	ID=Msa1356610-mRNA-1:exon:10049;Parent=Msa1356610-mRNA-1
tig0015812	five_prime_UTR	18077	18165	18077	ID=Msa1356610-mRNA-1:five_prime_utr;Parent=Msa1356610-mRNA-1
tig0015812	CDS	17597	18076	17597	ID=Msa1356610-mRNA-1:cds;Parent=Msa1356610-mRNA-1
tig0015812	CDS	16327	16572	16327	ID=Msa1356610-mRNA-1:cds;Parent=Msa1356610-mRNA-1
tig0015812	CDS	15349	15518	15349	ID=Msa1356610-mRNA-1:cds;Parent=Msa1356610-mRNA-1
tig0015812	CDS	14977	15193	14977	ID=Msa1356610-mRNA-1:cds;Parent=Msa1356610-mRNA-1
tig0015812	CDS	14736	14876	14736	ID=Msa1356610-mRNA-1:cds;Parent=Msa1356610-mRNA-1
tig0015812	CDS	13048	13131	13048	ID=Msa1356610-mRNA-1:cds;Parent=Msa1356610-mRNA-1
tig0015812	three_prime_UTR	12644	13047	12644	ID=Msa1356610-mRNA-1:three_prime_utr;Parent=Msa1356610-mRNA-1

Gene Sequence

>Msa1356610

ATGGGTCAAGGAACACCAGGAGGCGGTTTGAACCGGCAACCGGGTGACCGGAAACCAGACGGCAACGACAAAAAGGACAAGAAATTCGAACCGGCCGCACCACCGGCTCGCGTCGGTCGCAAACAGCGAAAACAAAAGGGTTCCGAGGCTGCGTCGCGGTTGCCGACGGTAACTCCGTTATCGAAGTGCAAACTTCGGTTACTGAAACTGGAACGAATCAAGGACTATTTGTTAATGGAAGAAGAGTTTGTAGCGAATCAGGAAAGGTTGAAGCCTCAGGAGGAGAAAGCTGAAGAAGATAGATCTAAGGTTGATGATCTTCGTGGTTCGCCTATGAGTGTTGGCAATCTTGAAGAGCTTATTGATGAGAATCATGCTATTGTTTCAAGTTCGGTTGGACCGGAGTATTATGTTGGGATTTTGTCCTTTGTTGATAAGGATCAGTTGGAACCTGGTTGCGCTATTTTGATGCATAACAAGGTTCTTTCTGTTGTTGGACTTCTTCAAGATGAAGTTGATCCAATGGTCTCTGTAATGAAGGTTGAGAAGGCTCCTTTGGAATCATATGCTGACATTGGTGGTTTAGATGCCCAGATACAGGAAATTAAAGAAGCTGTAGAGCTTCCCCTTACACATCCTGAACTGTATGAAGATATTGGTATCAAGCCTCCAAAGGGAGTCATTTTATATGGAGAACCTGGAACCGGAAAGACACTTCTTGCAAAGGCTGTGGCCAACTCAACATCAGCAACATTCTTGCGTGTTGTTGGTAGTGAGTTGATTCAAAAATACTTAGGAGATGGTCCAAAACTTGTGAGGGAACTTTTTCGAGTTGCTGATGATCTTTCTCCCTCTATTGTCTTTATTGATGAAATTGACGCTGTTGGTACAAAGAGGTATGATGCTCACTCAGGTGGAGAGCGTGAAATTCAAAGGACCATGTTGGAGTTGCTGAACCAGTTAGATGGTTTTGATTCTAGAGGAGATGTTAAAGTTATTCTCGCAACCAACAGAATTGAAAGCCTTGATCCAGCTCTGCTAAGACCAGGTCGAATAGACAGGAAAATTGAATTTCCTCTCCCTGATATCAAAACTAGGAGACGCATTTTCACGATACATACATCTAGGATGACATTAGCTGACGATGTCAATTTAGAAGAATTTGTCATGACTAAGGATGAGTTCTCTGGAGCTGATATAAAAGCAATATGTACCGAAGCTGGCTTACTTGCTTTACGAGAACGCCGAATGAAGGTGACCCATGCTGATTTTAAGAAGGCAAAAGATAAAGTTATGTTTAAGAAGAAAGAAGGGGTGCCTGAAGGATTGTATATGTGA

Protein sequence

>Msa1356610

MGQGTPGGGLNRQPGDRKPDGNDKKDKKFEPAAPPARVGRKQRKQKGSEAASRLPTVTPLSKCKLRLLKLERIKDYLLMEEEFVANQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKTLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPLPDIKTRRRIFTIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVTHADFKKAKDKVMFKKKEGVPEGLYM*