AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1300150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1300150 MtrunA17_Chr4g0061721 96.914 324 10 0 1 324 1 324 0.0 645
Msa1300150 MtrunA17_Chr4g0061731 91.692 325 26 1 1 324 1 325 0.0 605
Msa1300150 MtrunA17_Chr3g0134271 59.317 322 123 2 2 318 3 321 9.90e-132 378
Msa1300150 MtrunA17_Chr1g0152931 58.075 322 130 1 2 318 3 324 2.67e-130 374
Msa1300150 MtrunA17_Chr1g0198911 58.333 324 125 2 1 319 2 320 5.57e-129 370
Msa1300150 MtrunA17_Chr8g0349621 30.389 283 154 8 5 253 23 296 5.26e-26 107
Msa1300150 MtrunA17_Chr3g0079001 30.196 255 147 10 66 294 77 326 4.46e-17 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1300150 AT5G08100.1 73.209 321 77 2 1 320 2 314 3.00e-168 470
Msa1300150 AT3G16150.1 57.764 322 131 1 2 318 3 324 8.31e-132 378
Msa1300150 AT5G08100.2 73.967 242 55 1 79 320 1 234 1.45e-125 359
Msa1300150 AT4G00590.1 25.439 342 203 8 5 300 15 350 1.27e-20 92.0
Msa1300150 AT5G61540.1 29.130 230 132 8 66 269 80 304 3.15e-17 81.6
Msa1300150 AT4G00590.2 26.220 328 203 10 5 300 15 335 6.24e-17 81.3
Msa1300150 AT5G61540.3 28.085 235 135 10 89 294 1 230 7.64e-14 70.9
Msa1300150 AT5G61540.2 28.085 235 135 10 89 294 1 230 7.64e-14 70.9

Find 93 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTCTTGGTAATATCATTT+TGG 0.170201 8_3:-61608198 None:intergenic
GGTCGCGCATAGTCTTCTTT+AGG 0.214652 8_3:+61608790 Msa1300150:three_prime_UTR
ATGAAATGCGGTGCTGTTTC+TGG 0.224336 8_3:+61607193 Msa1300150:CDS
TGTTGTCAATGCTGTTTCTT+TGG 0.230934 8_3:+61607225 Msa1300150:CDS
TTCTTTGGCTCGTTTGGTTA+TGG 0.251608 8_3:+61607240 Msa1300150:CDS
TGCTGTTTCTTTGGCTCGTT+TGG 0.258446 8_3:+61607234 Msa1300150:CDS
AACATTCCTGGTGTATTATA+AGG 0.283990 8_3:-61608583 None:intergenic
ATCAGGCAAAAGAAGCAAAT+AGG 0.299059 8_3:+61608037 Msa1300150:CDS
GTCGCGCATAGTCTTCTTTA+GGG 0.303948 8_3:+61608791 Msa1300150:three_prime_UTR
TGTCATAACAAAATTAAGTT+TGG 0.306211 8_3:-61608693 None:intergenic
AACAAAATGGTTGGTCGAAT+TGG 0.316402 8_3:+61608331 Msa1300150:CDS
CATTTCAACCGTGCCTTTGT+TGG 0.327791 8_3:-61607143 None:intergenic
GGTATTCTGAAGTTGCAATT+TGG 0.344547 8_3:+61608629 Msa1300150:CDS
TCGCGTGGCTGACGGGCTTC+CGG 0.346128 8_3:-61606829 None:intergenic
TTAGTTGCTGTGTCTGCTGC+TGG 0.355395 8_3:+61608547 Msa1300150:CDS
TTATGTGCAGTTTCTGCAAC+AGG 0.369596 8_3:+61608394 Msa1300150:CDS
GAGCTTCTTCGCGTGGCTGA+CGG 0.371844 8_3:-61606837 None:intergenic
CAACAAAGGCACGGTTGAAA+TGG 0.373150 8_3:+61607144 Msa1300150:CDS
AGCTTCTTCGCGTGGCTGAC+GGG 0.383916 8_3:-61606836 None:intergenic
TGAAATGGAAGCTTCTATAA+TGG 0.384855 8_3:+61607159 Msa1300150:CDS
GCTAATGGTGATAGTCATCT+TGG 0.387149 8_3:+61608238 Msa1300150:CDS
TCGAGGTACATATTGTTGTT+TGG 0.397467 8_3:+61608769 Msa1300150:three_prime_UTR
ATTCAAAGGTCTTTCTCTAA+AGG 0.406833 8_3:+61608474 Msa1300150:CDS
ATTAAGTTTGGAAATCATAA+AGG 0.407021 8_3:-61608681 None:intergenic
CTAGAAATGAGAGGGTTGTT+AGG 0.411994 8_3:+61606482 Msa1300150:five_prime_UTR
AGAGCTTGTGCTACTGAAGA+TGG 0.415599 8_3:+61608607 Msa1300150:CDS
AAATATTGAAAGGCTAAATC+AGG 0.416374 8_3:+61608020 Msa1300150:CDS
TCAGGCAAAAGAAGCAAATA+GGG 0.422197 8_3:+61608038 Msa1300150:CDS
TGGTGTTTCTGTTTCACTCT+TGG 0.425615 8_3:-61608212 None:intergenic
CGCATACCAATGGGCACTGT+TGG 0.440706 8_3:+61608523 Msa1300150:CDS
CTAGCTTCAGCAACATCAAC+TGG 0.443431 8_3:+61608298 Msa1300150:CDS
ACATGTATGGTTATATCATA+AGG 0.444056 8_3:+61608731 Msa1300150:three_prime_UTR
GCACAAGCTCTAAACATTCC+TGG 0.447190 8_3:-61608595 None:intergenic
TGAGAGCTTTGACACCGATC+TGG 0.449887 8_3:-61606873 None:intergenic
ATTGGCGACACGCCACTCAT+AGG 0.458956 8_3:+61608349 Msa1300150:CDS
GTAGCACTACACGGCGGCGC+TGG 0.459640 8_3:+61606787 Msa1300150:CDS
AAACGTTAAGAATAGAAAGT+TGG 0.461857 8_3:+61606538 Msa1300150:five_prime_UTR
ACAGAAACACCATTTGCTAA+TGG 0.478619 8_3:+61608223 Msa1300150:CDS
CCACAATTCAATGCTGGTAG+AGG 0.483901 8_3:+61607109 Msa1300150:CDS
TTATGTTGATGATACATGTA+TGG 0.486191 8_3:+61608718 Msa1300150:three_prime_UTR
GAATATTGTTCCCCTCCAAA+TGG 0.487035 8_3:-61606324 None:intergenic
TGGTGGATTAGTGAACAAAA+TGG 0.500466 8_3:+61608318 Msa1300150:CDS
GCTTCCGGTGGAAGTGAACA+TGG 0.502741 8_3:-61606814 None:intergenic
TATGTACATTACAAAGTTCA+CGG 0.505636 8_3:+61606284 None:intergenic
AAGAGACGTGGCTGCAATGA+TGG 0.505654 8_3:+61608450 Msa1300150:CDS
GCAGTTTCTGCAACAGGGAT+AGG 0.517760 8_3:+61608400 Msa1300150:CDS
TCAAAAGCTAGATAAATATG+AGG 0.520309 8_3:-61607271 None:intergenic
TGTTCCCCTCCAAATGGAAA+AGG 0.521397 8_3:-61606318 None:intergenic
CACCGATCTGGAGGCAATGA+CGG 0.521864 8_3:-61606861 None:intergenic
GATAGTCATCTTGGAACTGT+TGG 0.524638 8_3:+61608247 Msa1300150:CDS
CTCCGTCATTGCCTCCAGAT+CGG 0.531000 8_3:+61606859 Msa1300150:CDS
TGAAAACTCACACACATAAG+TGG 0.531136 8_3:+61608858 Msa1300150:three_prime_UTR
TGACTATCACCATTAGCAAA+TGG 0.532229 8_3:-61608232 None:intergenic
CGTGGCTGACGGGCTTCCGG+TGG 0.536076 8_3:-61606826 None:intergenic
CGTCCCATGTTCACTTCCAC+CGG 0.538675 8_3:+61606810 Msa1300150:CDS
TTGTTCATAGGTTCGTGAGT+TGG 0.541945 8_3:+61607078 Msa1300150:intron
ATGGAAGCTTCTATAATGGA+TGG 0.542192 8_3:+61607163 Msa1300150:CDS
GTACACGAGCGCATACCAAT+GGG 0.545632 8_3:+61608514 Msa1300150:CDS
CAAGTTCAATGACGTCTAGA+GGG 0.547348 8_3:-61606909 None:intergenic
GACACGCCACTCATAGGTGC+TGG 0.548340 8_3:+61608355 Msa1300150:CDS
AATATTCCACAATTCAATGC+TGG 0.551168 8_3:+61607103 Msa1300150:CDS
GAGCTTGTGCTACTGAAGAT+GGG 0.553483 8_3:+61608608 Msa1300150:CDS
TGTACACGAGCGCATACCAA+TGG 0.562995 8_3:+61608513 Msa1300150:CDS
CTACTGCCCAGCCCATCAGT+TGG 0.565066 8_3:-61606768 None:intergenic
AAAAGAAGCAAATAGGGTCC+AGG 0.568354 8_3:+61608044 Msa1300150:CDS
AGAAACTGCACATAATTCGT+TGG 0.573046 8_3:-61608386 None:intergenic
TTACTCCAGAAAATATTGAA+AGG 0.577051 8_3:+61608010 Msa1300150:CDS
CCTCTACCAGCATTGAATTG+TGG 0.591538 8_3:-61607109 None:intergenic
ACAAGTTCAATGACGTCTAG+AGG 0.599325 8_3:-61606910 None:intergenic
TTGTATATCTAGAAATGAGA+GGG 0.600098 8_3:+61606474 Msa1300150:five_prime_UTR
GGAAATCTATTTGATTGTCG+AGG 0.601094 8_3:+61608752 Msa1300150:three_prime_UTR
TTTGTATATCTAGAAATGAG+AGG 0.603684 8_3:+61606473 Msa1300150:five_prime_UTR
GGTTCTGTTCTTACCAACAA+AGG 0.614531 8_3:+61607130 Msa1300150:CDS
ACTAAACCAACAGTGCCCAT+TGG 0.614693 8_3:-61608529 None:intergenic
TGACGGAGAGCTTCTTCGCG+TGG 0.615772 8_3:-61606844 None:intergenic
GGCTGGGCAGTAGCACTACA+CGG 0.619395 8_3:+61606778 Msa1300150:CDS
GAGCTTTGACACCGATCTGG+AGG 0.621212 8_3:-61606870 None:intergenic
GGATTAGTGAACAAAATGGT+TGG 0.625378 8_3:+61608322 Msa1300150:CDS
GCTTCAGCAACATCAACTGG+TGG 0.625484 8_3:+61608301 Msa1300150:CDS
CAAAGCTCTCAAATCCAACA+TGG 0.626979 8_3:+61606885 Msa1300150:CDS
TGTGTAGCTGTTGATAGCTA+CGG 0.628569 8_3:+61608271 Msa1300150:CDS
GCTGCAATGATGGAATTCAA+AGG 0.639555 8_3:+61608460 Msa1300150:CDS
TAGGCGAAGCACTAATACGC+GGG 0.640412 8_3:+61608419 Msa1300150:CDS
TATGTTCCAGCACCTATGAG+TGG 0.651676 8_3:-61608361 None:intergenic
GCAATGCCTTATAATACACC+AGG 0.652456 8_3:+61608577 Msa1300150:CDS
TGTTCTTACCAACAAAGGCA+CGG 0.657835 8_3:+61607135 Msa1300150:CDS
AAGTTCAATGACGTCTAGAG+GGG 0.662820 8_3:-61606908 None:intergenic
TATGTGCAGTTTCTGCAACA+GGG 0.671046 8_3:+61608395 Msa1300150:CDS
CTTCCGGTGGAAGTGAACAT+GGG 0.682054 8_3:-61606813 None:intergenic
CGGGACAGTAGCAAGAGACG+TGG 0.693457 8_3:+61608438 Msa1300150:CDS
GATGGAAATACTATGAAATG+CGG 0.693488 8_3:+61607181 Msa1300150:CDS
TGGGCAGTAGCACTACACGG+CGG 0.782765 8_3:+61606781 Msa1300150:CDS
ATAGGCGAAGCACTAATACG+CGG 0.787645 8_3:+61608418 Msa1300150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGTCATAAAAAATAATA+AGG - chr8_3:61606449-61606468 None:intergenic 10.0%
!!! TATTATTTTTTATGACATTT+CGG + chr8_3:61606449-61606468 Msa1300150:five_prime_UTR 10.0%
!!! TATTATATTAAAGTTTTGTT+TGG - chr8_3:61607621-61607640 None:intergenic 10.0%
!!! TTTCTCTCTTTTTTTTTTTT+TGG + chr8_3:61606406-61606425 Msa1300150:five_prime_UTR 15.0%
!!! TTCTCTCTTTTTTTTTTTTT+GGG + chr8_3:61606407-61606426 Msa1300150:five_prime_UTR 15.0%
!! TCTCAAAAATAGTAATTTAA+CGG - chr8_3:61606586-61606605 None:intergenic 15.0%
!! TATAAGTTATTCAATATGAT+AGG - chr8_3:61608140-61608159 None:intergenic 15.0%
!! ACCTAAAAATAAAAAAAATG+AGG - chr8_3:61608817-61608836 None:intergenic 15.0%
!!! CATTTTCTTTTATGAAAACT+TGG + chr8_3:61606964-61606983 Msa1300150:intron 20.0%
!! TCATAATAATCATTGTTCAT+AGG + chr8_3:61607066-61607085 Msa1300150:intron 20.0%
!! AAAACTTAAGAAAATGAGAT+TGG - chr8_3:61607342-61607361 None:intergenic 20.0%
!! TAAATAATGATCGTAGTTTA+TGG + chr8_3:61607918-61607937 Msa1300150:intron 20.0%
!! ATTAAGTTTGGAAATCATAA+AGG - chr8_3:61608684-61608703 None:intergenic 20.0%
!! TGTCATAACAAAATTAAGTT+TGG - chr8_3:61608696-61608715 None:intergenic 20.0%
!!! GCCTCATTTTTTTTATTTTT+AGG + chr8_3:61608813-61608832 Msa1300150:three_prime_UTR 20.0%
! TTTGTATATCTAGAAATGAG+AGG + chr8_3:61606473-61606492 Msa1300150:five_prime_UTR 25.0%
! TTGTATATCTAGAAATGAGA+GGG + chr8_3:61606474-61606493 Msa1300150:five_prime_UTR 25.0%
! AAACGTTAAGAATAGAAAGT+TGG + chr8_3:61606538-61606557 Msa1300150:five_prime_UTR 25.0%
!! TTTTATGAAAACTTGGTATC+CGG + chr8_3:61606971-61606990 Msa1300150:intron 25.0%
! TCAAAAGCTAGATAAATATG+AGG - chr8_3:61607274-61607293 None:intergenic 25.0%
!!! CATATTTATCTAGCTTTTGA+TGG + chr8_3:61607274-61607293 Msa1300150:CDS 25.0%
!! ATTCACTAACATAACCATTT+TGG + chr8_3:61607400-61607419 Msa1300150:intron 25.0%
! GAATGCATAAAGAAATACAT+AGG + chr8_3:61607824-61607843 Msa1300150:intron 25.0%
!!! ATCATATATCCTTATTTTGC+AGG + chr8_3:61607957-61607976 Msa1300150:intron 25.0%
!!! TCATATATCCTTATTTTGCA+GGG + chr8_3:61607958-61607977 Msa1300150:intron 25.0%
!!! TATTTTCTGGAGTAATGAAA+TGG - chr8_3:61608005-61608024 None:intergenic 25.0%
! TTACTCCAGAAAATATTGAA+AGG + chr8_3:61608010-61608029 Msa1300150:CDS 25.0%
!!! TTTAGCCTTTCAATATTTTC+TGG - chr8_3:61608018-61608037 None:intergenic 25.0%
! AAATATTGAAAGGCTAAATC+AGG + chr8_3:61608020-61608039 Msa1300150:CDS 25.0%
! TACTATGAAAGTTATATACC+TGG - chr8_3:61608065-61608084 None:intergenic 25.0%
! TATTCAATATGATAGGAGAA+GGG - chr8_3:61608133-61608152 None:intergenic 25.0%
! TTATTCAATATGATAGGAGA+AGG - chr8_3:61608134-61608153 None:intergenic 25.0%
!!! AATATCATTTTGGATTGGTT+GGG - chr8_3:61608191-61608210 None:intergenic 25.0%
!!! TAATATCATTTTGGATTGGT+TGG - chr8_3:61608192-61608211 None:intergenic 25.0%
!!! TTGGTAATATCATTTTGGAT+TGG - chr8_3:61608196-61608215 None:intergenic 25.0%
!!! TTATGTTGATGATACATGTA+TGG + chr8_3:61608718-61608737 Msa1300150:three_prime_UTR 25.0%
! ACATGTATGGTTATATCATA+AGG + chr8_3:61608731-61608750 Msa1300150:three_prime_UTR 25.0%
! TCTTAACGTTTTCTTTCGTA+TGG - chr8_3:61606529-61606548 None:intergenic 30.0%
AATCAGAAAAAACCAACTGA+TGG + chr8_3:61606756-61606775 Msa1300150:exon 30.0%
ATCAGAAAAAACCAACTGAT+GGG + chr8_3:61606757-61606776 Msa1300150:exon 30.0%
CAGAAGAAAGTTCAAATAGA+CGG - chr8_3:61607031-61607050 None:intergenic 30.0%
AATATTCCACAATTCAATGC+TGG + chr8_3:61607103-61607122 Msa1300150:CDS 30.0%
TGAAATGGAAGCTTCTATAA+TGG + chr8_3:61607159-61607178 Msa1300150:CDS 30.0%
GATGGAAATACTATGAAATG+CGG + chr8_3:61607181-61607200 Msa1300150:CDS 30.0%
! TTTCTTAAGTTTTCCCTTGT+AGG + chr8_3:61607349-61607368 Msa1300150:intron 30.0%
AATCCAAACAAATCCTACAA+GGG - chr8_3:61607365-61607384 None:intergenic 30.0%
ACTATCGTGATTGAAAACAT+TGG - chr8_3:61607666-61607685 None:intergenic 30.0%
! AATGTTTTCAATCACGATAG+TGG + chr8_3:61607665-61607684 Msa1300150:intron 30.0%
! AAATACATAGGTTTCTGAGA+AGG + chr8_3:61607836-61607855 Msa1300150:intron 30.0%
!!! CATATATCCTTATTTTGCAG+GGG + chr8_3:61607959-61607978 Msa1300150:intron 30.0%
!!! ATATATCCTTATTTTGCAGG+GGG + chr8_3:61607960-61607979 Msa1300150:intron 30.0%
ATTCAATATGATAGGAGAAG+GGG - chr8_3:61608132-61608151 None:intergenic 30.0%
!! CACTCTTGGTAATATCATTT+TGG - chr8_3:61608201-61608220 None:intergenic 30.0%
ATTCAAAGGTCTTTCTCTAA+AGG + chr8_3:61608474-61608493 Msa1300150:CDS 30.0%
AACATTCCTGGTGTATTATA+AGG - chr8_3:61608586-61608605 None:intergenic 30.0%
! GTATAGTTGCCTTTTCCATT+TGG + chr8_3:61606309-61606328 Msa1300150:five_prime_UTR 35.0%
AGAAAGTTCAAATAGACGGT+AGG - chr8_3:61607027-61607046 None:intergenic 35.0%
! ATGGAAGCTTCTATAATGGA+TGG + chr8_3:61607163-61607182 Msa1300150:CDS 35.0%
!! TGTTGTCAATGCTGTTTCTT+TGG + chr8_3:61607225-61607244 Msa1300150:CDS 35.0%
! GATTTTGCAAGACAACAAGT+TGG + chr8_3:61607304-61607323 Msa1300150:intron 35.0%
! TTTCCCTTGTAGGATTTGTT+TGG + chr8_3:61607359-61607378 Msa1300150:intron 35.0%
GAATCCAAACAAATCCTACA+AGG - chr8_3:61607366-61607385 None:intergenic 35.0%
! ACCATTTTGGATACTGTTTG+TGG + chr8_3:61607413-61607432 Msa1300150:intron 35.0%
! CCATTTTGGATACTGTTTGT+GGG + chr8_3:61607414-61607433 Msa1300150:intron 35.0%
GTGTATTGCAATCGTAATCA+AGG - chr8_3:61607758-61607777 None:intergenic 35.0%
ATCAGGCAAAAGAAGCAAAT+AGG + chr8_3:61608037-61608056 Msa1300150:CDS 35.0%
TCAGGCAAAAGAAGCAAATA+GGG + chr8_3:61608038-61608057 Msa1300150:CDS 35.0%
AGAAGGGGAAAAAACACATT+AGG - chr8_3:61608117-61608136 None:intergenic 35.0%
! ACAGAAACACCATTTGCTAA+TGG + chr8_3:61608223-61608242 Msa1300150:CDS 35.0%
TGACTATCACCATTAGCAAA+TGG - chr8_3:61608235-61608254 None:intergenic 35.0%
!! TGGTGGATTAGTGAACAAAA+TGG + chr8_3:61608318-61608337 Msa1300150:CDS 35.0%
! GGATTAGTGAACAAAATGGT+TGG + chr8_3:61608322-61608341 Msa1300150:CDS 35.0%
! AACAAAATGGTTGGTCGAAT+TGG + chr8_3:61608331-61608350 Msa1300150:CDS 35.0%
AGAAACTGCACATAATTCGT+TGG - chr8_3:61608389-61608408 None:intergenic 35.0%
!! GGTATTCTGAAGTTGCAATT+TGG + chr8_3:61608629-61608648 Msa1300150:CDS 35.0%
GGAAATCTATTTGATTGTCG+AGG + chr8_3:61608752-61608771 Msa1300150:three_prime_UTR 35.0%
! TCGAGGTACATATTGTTGTT+TGG + chr8_3:61608769-61608788 Msa1300150:three_prime_UTR 35.0%
! TAGTTGCCTTTTCCATTTGG+AGG + chr8_3:61606312-61606331 Msa1300150:five_prime_UTR 40.0%
! AGTTGCCTTTTCCATTTGGA+GGG + chr8_3:61606313-61606332 Msa1300150:five_prime_UTR 40.0%
GAATATTGTTCCCCTCCAAA+TGG - chr8_3:61606327-61606346 None:intergenic 40.0%
CTAGAAATGAGAGGGTTGTT+AGG + chr8_3:61606482-61606501 Msa1300150:five_prime_UTR 40.0%
!! AAAAAACCAACTGATGGGCT+GGG + chr8_3:61606762-61606781 Msa1300150:exon 40.0%
CAAAGCTCTCAAATCCAACA+TGG + chr8_3:61606885-61606904 Msa1300150:CDS 40.0%
AAGTTCAATGACGTCTAGAG+GGG - chr8_3:61606911-61606930 None:intergenic 40.0%
CAAGTTCAATGACGTCTAGA+GGG - chr8_3:61606912-61606931 None:intergenic 40.0%
ACAAGTTCAATGACGTCTAG+AGG - chr8_3:61606913-61606932 None:intergenic 40.0%
AAAACTTGGTATCCGGTCTA+AGG + chr8_3:61606978-61606997 Msa1300150:intron 40.0%
GGGTTAATTGTTCCTTAGAC+CGG - chr8_3:61606993-61607012 None:intergenic 40.0%
CTAAGGAACAATTAACCCAG+AGG + chr8_3:61606995-61607014 Msa1300150:intron 40.0%
TTGTTCATAGGTTCGTGAGT+TGG + chr8_3:61607078-61607097 Msa1300150:intron 40.0%
GGTTCTGTTCTTACCAACAA+AGG + chr8_3:61607130-61607149 Msa1300150:CDS 40.0%
TGTTCTTACCAACAAAGGCA+CGG + chr8_3:61607135-61607154 Msa1300150:CDS 40.0%
! TTCTTTGGCTCGTTTGGTTA+TGG + chr8_3:61607240-61607259 Msa1300150:CDS 40.0%
CCCACAAACAGTATCCAAAA+TGG - chr8_3:61607417-61607436 None:intergenic 40.0%
GCTCTCGAGGATATGTTATA+TGG + chr8_3:61607494-61607513 Msa1300150:intron 40.0%
GAGATGTGATCAATCAAACG+TGG + chr8_3:61607723-61607742 Msa1300150:intron 40.0%
ATCGCAGAGACGTAAAAACT+CGG + chr8_3:61607784-61607803 Msa1300150:intron 40.0%
TGCATTCTTTCTGCACTTAG+AGG - chr8_3:61607811-61607830 None:intergenic 40.0%
AACTAGTCACCTTGAATTGC+TGG + chr8_3:61607887-61607906 Msa1300150:intron 40.0%
AGAAATCCACCAGCAATTCA+AGG - chr8_3:61607899-61607918 None:intergenic 40.0%
AAAAGAAGCAAATAGGGTCC+AGG + chr8_3:61608044-61608063 Msa1300150:CDS 40.0%
! TGGTGTTTCTGTTTCACTCT+TGG - chr8_3:61608215-61608234 None:intergenic 40.0%
GCTAATGGTGATAGTCATCT+TGG + chr8_3:61608238-61608257 Msa1300150:CDS 40.0%
GATAGTCATCTTGGAACTGT+TGG + chr8_3:61608247-61608266 Msa1300150:CDS 40.0%
!! TGTGTAGCTGTTGATAGCTA+CGG + chr8_3:61608271-61608290 Msa1300150:CDS 40.0%
TTATGTGCAGTTTCTGCAAC+AGG + chr8_3:61608394-61608413 Msa1300150:CDS 40.0%
TATGTGCAGTTTCTGCAACA+GGG + chr8_3:61608395-61608414 Msa1300150:CDS 40.0%
! GCTGCAATGATGGAATTCAA+AGG + chr8_3:61608460-61608479 Msa1300150:CDS 40.0%
GCAATGCCTTATAATACACC+AGG + chr8_3:61608577-61608596 Msa1300150:CDS 40.0%
! GTTGCCTTTTCCATTTGGAG+GGG + chr8_3:61606314-61606333 Msa1300150:five_prime_UTR 45.0%
TGTTCCCCTCCAAATGGAAA+AGG - chr8_3:61606321-61606340 None:intergenic 45.0%
! GAAAAAACCAACTGATGGGC+TGG + chr8_3:61606761-61606780 Msa1300150:exon 45.0%
! CCTCTACCAGCATTGAATTG+TGG - chr8_3:61607112-61607131 None:intergenic 45.0%
CCACAATTCAATGCTGGTAG+AGG + chr8_3:61607109-61607128 Msa1300150:CDS 45.0%
CATTTCAACCGTGCCTTTGT+TGG - chr8_3:61607146-61607165 None:intergenic 45.0%
! CAACAAAGGCACGGTTGAAA+TGG + chr8_3:61607144-61607163 Msa1300150:CDS 45.0%
!! ATGAAATGCGGTGCTGTTTC+TGG + chr8_3:61607193-61607212 Msa1300150:CDS 45.0%
!! TGCTGTTTCTTTGGCTCGTT+TGG + chr8_3:61607234-61607253 Msa1300150:CDS 45.0%
!!! TCTAGCTTTTGATGGAGCTG+AGG + chr8_3:61607282-61607301 Msa1300150:CDS 45.0%
GAGAGCTCATGGAAATAAGC+TGG - chr8_3:61607480-61607499 None:intergenic 45.0%
CTTATTTCCATGAGCTCTCG+AGG + chr8_3:61607481-61607500 Msa1300150:intron 45.0%
AACATATCCTCGAGAGCTCA+TGG - chr8_3:61607491-61607510 None:intergenic 45.0%
TCAATCAAACGTGGCTCATG+TGG + chr8_3:61607732-61607751 Msa1300150:intron 45.0%
! TCTGAGAAGGAGCATACATG+TGG + chr8_3:61607849-61607868 Msa1300150:intron 45.0%
TAGTCACCTTGAATTGCTGG+TGG + chr8_3:61607890-61607909 Msa1300150:intron 45.0%
TCTCAACCCCCTGCAAAATA+AGG - chr8_3:61607969-61607988 None:intergenic 45.0%
CTAGCTTCAGCAACATCAAC+TGG + chr8_3:61608298-61608317 Msa1300150:CDS 45.0%
TATGTTCCAGCACCTATGAG+TGG - chr8_3:61608364-61608383 None:intergenic 45.0%
! ATAGGCGAAGCACTAATACG+CGG + chr8_3:61608418-61608437 Msa1300150:CDS 45.0%
ACTAAACCAACAGTGCCCAT+TGG - chr8_3:61608532-61608551 None:intergenic 45.0%
!! GCACAAGCTCTAAACATTCC+TGG - chr8_3:61608598-61608617 None:intergenic 45.0%
! AGAGCTTGTGCTACTGAAGA+TGG + chr8_3:61608607-61608626 Msa1300150:CDS 45.0%
! GAGCTTGTGCTACTGAAGAT+GGG + chr8_3:61608608-61608627 Msa1300150:CDS 45.0%
GTCGCGCATAGTCTTCTTTA+GGG + chr8_3:61608791-61608810 Msa1300150:three_prime_UTR 45.0%
CTTCCGGTGGAAGTGAACAT+GGG - chr8_3:61606816-61606835 None:intergenic 50.0%
! TGAGAGCTTTGACACCGATC+TGG - chr8_3:61606876-61606895 None:intergenic 50.0%
GACGGTAGGATTAGACCTCT+GGG - chr8_3:61607013-61607032 None:intergenic 50.0%
AGACGGTAGGATTAGACCTC+TGG - chr8_3:61607014-61607033 None:intergenic 50.0%
GCTTCAGCAACATCAACTGG+TGG + chr8_3:61608301-61608320 Msa1300150:CDS 50.0%
GCAGTTTCTGCAACAGGGAT+AGG + chr8_3:61608400-61608419 Msa1300150:CDS 50.0%
! TAGGCGAAGCACTAATACGC+GGG + chr8_3:61608419-61608438 Msa1300150:CDS 50.0%
AAGAGACGTGGCTGCAATGA+TGG + chr8_3:61608450-61608469 Msa1300150:CDS 50.0%
TGTACACGAGCGCATACCAA+TGG + chr8_3:61608513-61608532 Msa1300150:CDS 50.0%
GTACACGAGCGCATACCAAT+GGG + chr8_3:61608514-61608533 Msa1300150:CDS 50.0%
TTAGTTGCTGTGTCTGCTGC+TGG + chr8_3:61608547-61608566 Msa1300150:CDS 50.0%
GGTCGCGCATAGTCTTCTTT+AGG + chr8_3:61608790-61608809 Msa1300150:three_prime_UTR 50.0%
CGTCCCATGTTCACTTCCAC+CGG + chr8_3:61606810-61606829 Msa1300150:CDS 55.0%
GCTTCCGGTGGAAGTGAACA+TGG - chr8_3:61606817-61606836 None:intergenic 55.0%
CTCCGTCATTGCCTCCAGAT+CGG + chr8_3:61606859-61606878 Msa1300150:CDS 55.0%
CACCGATCTGGAGGCAATGA+CGG - chr8_3:61606864-61606883 None:intergenic 55.0%
! GAGCTTTGACACCGATCTGG+AGG - chr8_3:61606873-61606892 None:intergenic 55.0%
ATTGGCGACACGCCACTCAT+AGG + chr8_3:61608349-61608368 Msa1300150:CDS 55.0%
! CGCATACCAATGGGCACTGT+TGG + chr8_3:61608523-61608542 Msa1300150:CDS 55.0%
CTACTGCCCAGCCCATCAGT+TGG - chr8_3:61606771-61606790 None:intergenic 60.0%
GGCTGGGCAGTAGCACTACA+CGG + chr8_3:61606778-61606797 Msa1300150:CDS 60.0%
TGGGCAGTAGCACTACACGG+CGG + chr8_3:61606781-61606800 Msa1300150:CDS 60.0%
AGCTTCTTCGCGTGGCTGAC+GGG - chr8_3:61606839-61606858 None:intergenic 60.0%
GAGCTTCTTCGCGTGGCTGA+CGG - chr8_3:61606840-61606859 None:intergenic 60.0%
TGACGGAGAGCTTCTTCGCG+TGG - chr8_3:61606847-61606866 None:intergenic 60.0%
GACGTCTAGAGGGGCCATGT+TGG - chr8_3:61606902-61606921 None:intergenic 60.0%
GACACGCCACTCATAGGTGC+TGG + chr8_3:61608355-61608374 Msa1300150:CDS 60.0%
CGGGACAGTAGCAAGAGACG+TGG + chr8_3:61608438-61608457 Msa1300150:CDS 60.0%
GTAGCACTACACGGCGGCGC+TGG + chr8_3:61606787-61606806 Msa1300150:CDS 70.0%
!! TCGCGTGGCTGACGGGCTTC+CGG - chr8_3:61606832-61606851 None:intergenic 70.0%
!! CGTGGCTGACGGGCTTCCGG+TGG - chr8_3:61606829-61606848 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 61606296 61608879 61606296 ID=Msa1300150;Name=Msa1300150
chr8_3 mRNA 61606296 61608879 61606296 ID=Msa1300150-mRNA-1;Parent=Msa1300150;Name=Msa1300150-mRNA-1;_AED=0.30;_eAED=0.30;_QI=479|1|1|1|1|1|4|207|324
chr8_3 exon 61606296 61606933 61606296 ID=Msa1300150-mRNA-1:exon:18213;Parent=Msa1300150-mRNA-1
chr8_3 exon 61607088 61607321 61607088 ID=Msa1300150-mRNA-1:exon:18214;Parent=Msa1300150-mRNA-1
chr8_3 exon 61607979 61608065 61607979 ID=Msa1300150-mRNA-1:exon:18215;Parent=Msa1300150-mRNA-1
chr8_3 exon 61608178 61608879 61608178 ID=Msa1300150-mRNA-1:exon:18216;Parent=Msa1300150-mRNA-1
chr8_3 five_prime_UTR 61606296 61606774 61606296 ID=Msa1300150-mRNA-1:five_prime_utr;Parent=Msa1300150-mRNA-1
chr8_3 CDS 61606775 61606933 61606775 ID=Msa1300150-mRNA-1:cds;Parent=Msa1300150-mRNA-1
chr8_3 CDS 61607088 61607321 61607088 ID=Msa1300150-mRNA-1:cds;Parent=Msa1300150-mRNA-1
chr8_3 CDS 61607979 61608065 61607979 ID=Msa1300150-mRNA-1:cds;Parent=Msa1300150-mRNA-1
chr8_3 CDS 61608178 61608672 61608178 ID=Msa1300150-mRNA-1:cds;Parent=Msa1300150-mRNA-1
chr8_3 three_prime_UTR 61608673 61608879 61608673 ID=Msa1300150-mRNA-1:three_prime_utr;Parent=Msa1300150-mRNA-1
Gene Sequence

>Msa1300150

ATGGGCTGGGCAGTAGCACTACACGGCGGCGCTGGCGTCCCATGTTCACTTCCACCGGAAGCCCGTCAGCCACGCGAAGAAGCTCTCCGTCATTGCCTCCAGATCGGTGTCAAAGCTCTCAAATCCAACATGGCCCCTCTAGACGTCATTGAACTTGTTGTTCGTGAGTTGGAGAATATTCCACAATTCAATGCTGGTAGAGGTTCTGTTCTTACCAACAAAGGCACGGTTGAAATGGAAGCTTCTATAATGGATGGAAATACTATGAAATGCGGTGCTGTTTCTGGTCTTAGCACTGTTGTCAATGCTGTTTCTTTGGCTCGTTTGGTTATGGATAATACTCCTCATATTTATCTAGCTTTTGATGGAGCTGAGGATTTTGCAAGACAACAAGGGGTTGAGACTGTAGATACAAGCCATTTCATTACTCCAGAAAATATTGAAAGGCTAAATCAGGCAAAAGAAGCAAATAGGGTCCAGATTGATTATACCCAACCAATCCAAAATGATATTACCAAGAGTGAAACAGAAACACCATTTGCTAATGGTGATAGTCATCTTGGAACTGTTGGATGTGTAGCTGTTGATAGCTACGGAAATCTAGCTTCAGCAACATCAACTGGTGGATTAGTGAACAAAATGGTTGGTCGAATTGGCGACACGCCACTCATAGGTGCTGGAACATATGCCAACGAATTATGTGCAGTTTCTGCAACAGGGATAGGCGAAGCACTAATACGCGGGACAGTAGCAAGAGACGTGGCTGCAATGATGGAATTCAAAGGTCTTTCTCTAAAGGAAGCTGCTGATTGTGTTGTACACGAGCGCATACCAATGGGCACTGTTGGTTTAGTTGCTGTGTCTGCTGCTGGAGAAGTTGCAATGCCTTATAATACACCAGGAATGTTTAGAGCTTGTGCTACTGAAGATGGGTATTCTGAAGTTGCAATTTGGTCAGACGCTATAATTGAGTGA
Protein sequence

>Msa1300150

MGWAVALHGGAGVPCSLPPEARQPREEALRHCLQIGVKALKSNMAPLDVIELVVRELENIPQFNAGRGSVLTNKGTVEMEASIMDGNTMKCGAVSGLSTVVNAVSLARLVMDNTPHIYLAFDGAEDFARQQGVETVDTSHFITPENIERLNQAKEANRVQIDYTQPIQNDITKSETETPFANGDSHLGTVGCVAVDSYGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSATGIGEALIRGTVARDVAAMMEFKGLSLKEAADCVVHERIPMGTVGLVAVSAAGEVAMPYNTPGMFRACATEDGYSEVAIWSDAIIE*