Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0280570 | XP_039686033.1 | 96.154 | 52 | 0 | 1 | 1 | 52 | 1 | 50 | 2.90e-24 | 96.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0280570 | A0A396J551 | 96.154 | 52 | 0 | 1 | 1 | 52 | 1 | 50 | 1.39e-24 | 96.3 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000550 | Msa0280570 | -0.809747 | 1.623736e-50 | -8.615850e-47 |
| Msa0004790 | Msa0280570 | -0.807318 | 5.360298e-50 | -8.615850e-47 |
| Msa0015390 | Msa0280570 | 0.810997 | 8.726477e-51 | -8.615850e-47 |
| Msa0033440 | Msa0280570 | 0.825195 | 5.351351e-54 | -8.615850e-47 |
| Msa0048000 | Msa0280570 | 0.826191 | 3.105351e-54 | -8.615850e-47 |
| Msa0050730 | Msa0280570 | 0.806313 | 8.739200e-50 | -8.615850e-47 |
| Msa0050760 | Msa0280570 | 0.811791 | 5.866355e-51 | -8.615850e-47 |
| Msa0069310 | Msa0280570 | 0.820891 | 5.398230e-53 | -8.615850e-47 |
| Msa0092990 | Msa0280570 | 0.804846 | 1.775228e-49 | -8.615850e-47 |
| Msa0133980 | Msa0280570 | -0.809386 | 1.941814e-50 | -8.615850e-47 |
| Msa0140860 | Msa0280570 | 0.820669 | 6.069044e-53 | -8.615850e-47 |
| Msa0197660 | Msa0280570 | 0.824178 | 9.291987e-54 | -8.615850e-47 |
| Msa0199820 | Msa0280570 | 0.966867 | 1.692140e-126 | -8.615850e-47 |
| Msa0206790 | Msa0280570 | 0.812350 | 4.431863e-51 | -8.615850e-47 |
| Msa0213040 | Msa0280570 | 0.821314 | 4.313392e-53 | -8.615850e-47 |
| Msa0215320 | Msa0280570 | -0.810419 | 1.163662e-50 | -8.615850e-47 |
| Msa0217400 | Msa0280570 | 0.838502 | 2.768274e-57 | -8.615850e-47 |
| Msa0223410 | Msa0280570 | -0.807588 | 4.696503e-50 | -8.615850e-47 |
| Msa0233300 | Msa0280570 | 0.831133 | 1.983836e-55 | -8.615850e-47 |
| Msa0239800 | Msa0280570 | 0.820386 | 7.050964e-53 | -8.615850e-47 |
| Msa0249560 | Msa0280570 | 0.823125 | 1.638930e-53 | -8.615850e-47 |
| Msa0253340 | Msa0280570 | 0.814035 | 1.891174e-51 | -8.615850e-47 |
| Msa0258760 | Msa0280570 | 0.826622 | 2.452137e-54 | -8.615850e-47 |
| Msa0260810 | Msa0280570 | 0.837453 | 5.152853e-57 | -8.615850e-47 |
| Msa0269450 | Msa0280570 | 0.815444 | 9.217608e-52 | -8.615850e-47 |
| Msa0280570 | Msa0292170 | 0.831128 | 1.989689e-55 | -8.615850e-47 |
| Msa0280570 | Msa0296560 | 0.809804 | 1.579078e-50 | -8.615850e-47 |
| Msa0280570 | Msa0302580 | -0.802547 | 5.326942e-49 | -8.615850e-47 |
| Msa0280570 | Msa0306210 | -0.813868 | 2.058568e-51 | -8.615850e-47 |
| Msa0280570 | Msa0317510 | 0.823115 | 1.647695e-53 | -8.615850e-47 |
| Msa0280570 | Msa0320100 | 0.801439 | 8.998819e-49 | -8.615850e-47 |
| Msa0280570 | Msa0323080 | 0.856421 | 3.255053e-62 | -8.615850e-47 |
| Msa0280570 | Msa0326520 | 0.827780 | 1.295147e-54 | -8.615850e-47 |
| Msa0280570 | Msa0330390 | 0.818502 | 1.896179e-52 | -8.615850e-47 |
| Msa0280570 | Msa0335040 | 0.851973 | 6.258210e-61 | -8.615850e-47 |
| Msa0280570 | Msa0340450 | 0.810687 | 1.018313e-50 | -8.615850e-47 |
| Msa0280570 | Msa0344690 | -0.809489 | 1.844799e-50 | -8.615850e-47 |
| Msa0280570 | Msa0344830 | 0.833190 | 6.148028e-56 | -8.615850e-47 |
| Msa0280570 | Msa0350990 | 0.810643 | 1.041037e-50 | -8.615850e-47 |
| Msa0280570 | Msa0351030 | 0.811741 | 6.014556e-51 | -8.615850e-47 |
| Msa0280570 | Msa0377530 | 0.823253 | 1.529654e-53 | -8.615850e-47 |
| Msa0280570 | Msa0392040 | -0.807929 | 3.976262e-50 | -8.615850e-47 |
| Msa0280570 | Msa0402820 | 0.810364 | 1.196044e-50 | -8.615850e-47 |
| Msa0280570 | Msa0414950 | -0.809335 | 1.990950e-50 | -8.615850e-47 |
| Msa0280570 | Msa0436280 | -0.805164 | 1.523587e-49 | -8.615850e-47 |
| Msa0280570 | Msa0442850 | 0.811503 | 6.776243e-51 | -8.615850e-47 |
| Msa0280570 | Msa0445280 | 0.803210 | 3.887140e-49 | -8.615850e-47 |
| Msa0280570 | Msa0449760 | 0.819304 | 1.246212e-52 | -8.615850e-47 |
| Msa0280570 | Msa0449830 | 0.819595 | 1.069554e-52 | -8.615850e-47 |
| Msa0280570 | Msa0458440 | 0.800091 | 1.695517e-48 | -8.615850e-47 |
| Msa0280570 | Msa0464900 | 0.805610 | 1.228274e-49 | -8.615850e-47 |
| Msa0280570 | Msa0466390 | 0.811647 | 6.305898e-51 | -8.615850e-47 |
| Msa0280570 | Msa0469940 | -0.816157 | 6.390980e-52 | -8.615850e-47 |
| Msa0280570 | Msa0473240 | 0.829592 | 4.722622e-55 | -8.615850e-47 |
| Msa0280570 | Msa0479340 | 0.817143 | 3.843064e-52 | -8.615850e-47 |
| Msa0280570 | Msa0482190 | -0.801004 | 1.104616e-48 | -8.615850e-47 |
| Msa0280570 | Msa0484780 | 0.833610 | 4.830080e-56 | -8.615850e-47 |
| Msa0280570 | Msa0492880 | 0.804748 | 1.861709e-49 | -8.615850e-47 |
| Msa0280570 | Msa0495330 | 0.811031 | 8.579747e-51 | -8.615850e-47 |
| Msa0280570 | Msa0517490 | 0.830348 | 3.089887e-55 | -8.615850e-47 |
| Msa0280570 | Msa0535060 | 0.803674 | 3.113914e-49 | -8.615850e-47 |
| Msa0280570 | Msa0558690 | 0.826032 | 3.388111e-54 | -8.615850e-47 |
| Msa0280570 | Msa0562440 | 0.849806 | 2.553060e-60 | -8.615850e-47 |
| Msa0280570 | Msa0567020 | 0.807542 | 4.805161e-50 | -8.615850e-47 |
| Msa0280570 | Msa0578970 | 0.801093 | 1.059531e-48 | -8.615850e-47 |
| Msa0280570 | Msa0579390 | 0.807066 | 6.059819e-50 | -8.615850e-47 |
| Msa0280570 | Msa0585230 | 0.800561 | 1.360077e-48 | -8.615850e-47 |
| Msa0280570 | Msa0587690 | 0.820233 | 7.641923e-53 | -8.615850e-47 |
| Msa0280570 | Msa0610590 | 0.800644 | 1.308583e-48 | -8.615850e-47 |
| Msa0280570 | Msa0613460 | 0.848144 | 7.389882e-60 | -8.615850e-47 |
| Msa0280570 | Msa0621590 | 0.810381 | 1.185814e-50 | -8.615850e-47 |
| Msa0280570 | Msa0623080 | 0.826382 | 2.796711e-54 | -8.615850e-47 |
| Msa0280570 | Msa0627910 | 0.803821 | 2.903157e-49 | -8.615850e-47 |
| Msa0280570 | Msa0638280 | 0.812757 | 3.609551e-51 | -8.615850e-47 |
| Msa0280570 | Msa0638320 | 0.803203 | 3.899566e-49 | -8.615850e-47 |
| Msa0280570 | Msa0651190 | 0.803803 | 2.928199e-49 | -8.615850e-47 |
| Msa0280570 | Msa0654010 | 0.832993 | 6.882966e-56 | -8.615850e-47 |
| Msa0280570 | Msa0658570 | 0.815144 | 1.074602e-51 | -8.615850e-47 |
| Msa0280570 | Msa0662650 | 0.817214 | 3.702835e-52 | -8.615850e-47 |
| Msa0280570 | Msa0689010 | 0.810931 | 9.017423e-51 | -8.615850e-47 |
| Msa0280570 | Msa0693380 | 0.827892 | 1.216892e-54 | -8.615850e-47 |
| Msa0280570 | Msa0708860 | 0.816320 | 5.876132e-52 | -8.615850e-47 |
| Msa0280570 | Msa0743680 | 0.822832 | 1.918057e-53 | -8.615850e-47 |
| Msa0280570 | Msa0744170 | -0.805182 | 1.510640e-49 | -8.615850e-47 |
| Msa0280570 | Msa0784380 | 0.810592 | 1.067901e-50 | -8.615850e-47 |
| Msa0280570 | Msa0846810 | 0.810171 | 1.315835e-50 | -8.615850e-47 |
| Msa0280570 | Msa0857640 | 0.813329 | 2.704604e-51 | -8.615850e-47 |
| Msa0280570 | Msa0863800 | 0.806531 | 7.862239e-50 | -8.615850e-47 |
| Msa0280570 | Msa0870530 | 0.800953 | 1.131549e-48 | -8.615850e-47 |
| Msa0280570 | Msa0907070 | 0.820391 | 7.030879e-53 | -8.615850e-47 |
| Msa0280570 | Msa0908170 | 0.824192 | 9.223612e-54 | -8.615850e-47 |
| Msa0280570 | Msa0910980 | -0.805384 | 1.370170e-49 | -8.615850e-47 |
| Msa0280570 | Msa0922410 | 0.800405 | 1.464025e-48 | -8.615850e-47 |
| Msa0280570 | Msa0935910 | 0.804972 | 1.671443e-49 | -8.615850e-47 |
| Msa0280570 | Msa0936300 | 0.834473 | 2.936799e-56 | -8.615850e-47 |
| Msa0280570 | Msa0942550 | -0.801499 | 8.749938e-49 | -8.615850e-47 |
| Msa0280570 | Msa0956390 | 0.808171 | 3.531630e-50 | -8.615850e-47 |
| Msa0280570 | Msa0973100 | 0.817070 | 3.990203e-52 | -8.615850e-47 |
| Msa0280570 | Msa0976250 | 0.814209 | 1.731419e-51 | -8.615850e-47 |
| Msa0280570 | Msa0991670 | 0.816800 | 4.588332e-52 | -8.615850e-47 |
| Msa0280570 | Msa0992490 | -0.831110 | 2.009821e-55 | -8.615850e-47 |
| Msa0280570 | Msa1021340 | 0.812952 | 3.271866e-51 | -8.615850e-47 |
| Msa0280570 | Msa1027290 | 0.824721 | 6.924966e-54 | -8.615850e-47 |
| Msa0280570 | Msa1028270 | 0.815713 | 8.028461e-52 | -8.615850e-47 |
| Msa0280570 | Msa1039880 | 0.820858 | 5.492413e-53 | -8.615850e-47 |
| Msa0280570 | Msa1073070 | -0.804879 | 1.747363e-49 | -8.615850e-47 |
| Msa0280570 | Msa1075220 | -0.836225 | 1.060265e-56 | -8.615850e-47 |
| Msa0280570 | Msa1082500 | 0.819113 | 1.377358e-52 | -8.615850e-47 |
| Msa0280570 | Msa1082580 | 0.813456 | 2.535991e-51 | -8.615850e-47 |
| Msa0280570 | Msa1087470 | 0.837395 | 5.332760e-57 | -8.615850e-47 |
| Msa0280570 | Msa1095480 | 0.831947 | 1.250078e-55 | -8.615850e-47 |
| Msa0280570 | Msa1109570 | 0.821174 | 4.644717e-53 | -8.615850e-47 |
| Msa0280570 | Msa1116130 | -0.813208 | 2.875867e-51 | -8.615850e-47 |
| Msa0280570 | Msa1130270 | -0.808001 | 3.838278e-50 | -8.615850e-47 |
| Msa0280570 | Msa1136130 | 0.800137 | 1.659801e-48 | -8.615850e-47 |
| Msa0280570 | Msa1139000 | -0.815173 | 1.058734e-51 | -8.615850e-47 |
| Msa0280570 | Msa1144720 | -0.809439 | 1.891510e-50 | -8.615850e-47 |
| Msa0280570 | Msa1162550 | -0.845703 | 3.442746e-59 | -8.615850e-47 |
| Msa0280570 | Msa1195920 | 0.804924 | 1.709796e-49 | -8.615850e-47 |
| Msa0280570 | Msa1211150 | -0.805667 | 1.194949e-49 | -8.615850e-47 |
| Msa0280570 | Msa1228210 | 0.807621 | 4.621313e-50 | -8.615850e-47 |
| Msa0280570 | Msa1237030 | 0.803770 | 2.974243e-49 | -8.615850e-47 |
| Msa0280570 | Msa1238930 | 0.813387 | 2.626625e-51 | -8.615850e-47 |
| Msa0280570 | Msa1244750 | -0.807125 | 5.887269e-50 | -8.615850e-47 |
| Msa0280570 | Msa1264020 | 0.800674 | 1.290051e-48 | -8.615850e-47 |
| Msa0280570 | Msa1267060 | 0.801179 | 1.017476e-48 | -8.615850e-47 |
| Msa0280570 | Msa1269580 | -0.801392 | 9.199656e-49 | -8.615850e-47 |
| Msa0280570 | Msa1283860 | 0.806253 | 8.997657e-50 | -8.615850e-47 |
| Msa0280570 | Msa1284680 | 0.803585 | 3.249393e-49 | -8.615850e-47 |
| Msa0280570 | Msa1300150 | -0.805152 | 1.531969e-49 | -8.615850e-47 |
| Msa0280570 | Msa1310210 | 0.813252 | 2.812230e-51 | -8.615850e-47 |
| Msa0280570 | Msa1322890 | 0.806670 | 7.350312e-50 | -8.615850e-47 |
| Msa0280570 | Msa1333990 | 0.804991 | 1.655669e-49 | -8.615850e-47 |
| Msa0280570 | Msa1336490 | 0.808356 | 3.224693e-50 | -8.615850e-47 |
| Msa0280570 | Msa1340650 | 0.801822 | 7.509084e-49 | -8.615850e-47 |
| Msa0280570 | Msa1348950 | 0.801111 | 1.050655e-48 | -8.615850e-47 |
| Msa0280570 | Msa1352540 | -0.802609 | 5.172472e-49 | -8.615850e-47 |
| Msa0280570 | Msa1359650 | -0.811453 | 6.947081e-51 | -8.615850e-47 |
| Msa0280570 | Msa1362880 | 0.855902 | 4.619109e-62 | -8.615850e-47 |
| Msa0280570 | Msa1363890 | 0.807348 | 5.282888e-50 | -8.615850e-47 |
| Msa0280570 | Msa1369530 | 0.843356 | 1.473518e-58 | -8.615850e-47 |
| Msa0280570 | Msa1376890 | 0.819020 | 1.445697e-52 | -8.615850e-47 |
| Msa0280570 | Msa1376930 | 0.801495 | 8.765618e-49 | -8.615850e-47 |
| Msa0280570 | Msa1384350 | 0.801246 | 9.856354e-49 | -8.615850e-47 |
| Msa0280570 | Msa1390120 | 0.840057 | 1.093354e-57 | -8.615850e-47 |
| Msa0280570 | Msa1393040 | 0.802245 | 6.148187e-49 | -8.615850e-47 |
| Msa0280570 | Msa1393410 | 0.802490 | 5.472812e-49 | -8.615850e-47 |
| Msa0280570 | Msa1395530 | 0.838948 | 2.122512e-57 | -8.615850e-47 |
| Msa0280570 | Msa1396780 | 0.803802 | 2.929970e-49 | -8.615850e-47 |
| Msa0280570 | Msa1396830 | 0.824534 | 7.660316e-54 | -8.615850e-47 |
| Msa0280570 | Msa1404540 | 0.829430 | 5.169277e-55 | -8.615850e-47 |
| Msa0280570 | Msa1408790 | 0.838687 | 2.479845e-57 | -8.615850e-47 |
| Msa0280570 | Msa1429360 | -0.805915 | 1.059918e-49 | -8.615850e-47 |
| Msa0280570 | Msa1431060 | 0.823006 | 1.747564e-53 | -8.615850e-47 |
| Msa0280570 | Msa1435420 | 0.802293 | 6.008993e-49 | -8.615850e-47 |
| Msa0280570 | Msa1436040 | 0.832176 | 1.097406e-55 | -8.615850e-47 |
| Msa0280570 | Msa1440600 | 0.811593 | 6.479861e-51 | -8.615850e-47 |
| Msa0280570 | Msa1451620 | -0.835346 | 1.770600e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0280570 | MtrunA17_Chr2g0294531 | 96.154 | 52 | 0 | 1 | 1 | 52 | 1 | 50 | 2.67e-28 | 96.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACTTTGAATTTGAAGCAAT+CGG | 0.406582 | 2_3:-15407570 | None:intergenic |
| CTCAACTGTTGATCTTTGCA+CGG | 0.434979 | 2_3:-15407699 | None:intergenic |
| CATTCAACAACCATGGCAAC+GGG | 0.455582 | 2_3:+15407662 | Msa0280570:CDS |
| TGAGTTTGCAGTGCCATGAT+TGG | 0.461841 | 2_3:-15407641 | None:intergenic |
| AGTCTTGCTTCCCGTTGCCA+TGG | 0.528592 | 2_3:-15407672 | None:intergenic |
| TCATTCAACAACCATGGCAA+CGG | 0.561765 | 2_3:+15407661 | Msa0280570:CDS |
| TCAACTGTTGATCTTTGCAC+GGG | 0.562450 | 2_3:-15407698 | None:intergenic |
| AAACTTCTTGAGACTGAGGA+AGG | 0.625104 | 2_3:+15407598 | Msa0280570:CDS |
| GCAAACTCATTCAACAACCA+TGG | 0.640162 | 2_3:+15407655 | Msa0280570:CDS |
| ACGCAAACTTCTTGAGACTG+AGG | 0.679118 | 2_3:+15407594 | Msa0280570:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GACTTTGAATTTGAAGCAAT+CGG | - | chr2_3:15407573-15407592 | None:intergenic | 30.0% |
| ! | GAACTTTTCAACTCCAATCA+TGG | + | chr2_3:15407628-15407647 | Msa0280570:CDS | 35.0% |
| AAACTTCTTGAGACTGAGGA+AGG | + | chr2_3:15407598-15407617 | Msa0280570:CDS | 40.0% | |
| GCAAACTCATTCAACAACCA+TGG | + | chr2_3:15407655-15407674 | Msa0280570:CDS | 40.0% | |
| TCATTCAACAACCATGGCAA+CGG | + | chr2_3:15407661-15407680 | Msa0280570:CDS | 40.0% | |
| TCAACTGTTGATCTTTGCAC+GGG | - | chr2_3:15407701-15407720 | None:intergenic | 40.0% | |
| ACGCAAACTTCTTGAGACTG+AGG | + | chr2_3:15407594-15407613 | Msa0280570:CDS | 45.0% | |
| TGAGTTTGCAGTGCCATGAT+TGG | - | chr2_3:15407644-15407663 | None:intergenic | 45.0% | |
| CATTCAACAACCATGGCAAC+GGG | + | chr2_3:15407662-15407681 | Msa0280570:CDS | 45.0% | |
| AGTCTTGCTTCCCGTTGCCA+TGG | - | chr2_3:15407675-15407694 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 15407562 | 15407720 | 15407562 | ID=Msa0280570;Name=Msa0280570 |
| chr2_3 | mRNA | 15407562 | 15407720 | 15407562 | ID=Msa0280570-mRNA-1;Parent=Msa0280570;Name=Msa0280570-mRNA-1;_AED=0.62;_eAED=0.81;_QI=0|-1|0|1|-1|1|1|0|52 |
| chr2_3 | exon | 15407562 | 15407720 | 15407562 | ID=Msa0280570-mRNA-1:exon:6408;Parent=Msa0280570-mRNA-1 |
| chr2_3 | CDS | 15407562 | 15407720 | 15407562 | ID=Msa0280570-mRNA-1:cds;Parent=Msa0280570-mRNA-1 |
| Gene Sequence |
| Protein sequence |
