AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1303480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1303480 MtrunA17_Chr4g0071311 96.599 147 4 1 1 146 1 147 4.77e-99 281
Msa1303480 MtrunA17_Chr8g0341971 40.441 136 58 4 16 144 62 181 2.33e-20 82.4
Msa1303480 MtrunA17_Chr8g0341991 66.667 51 17 0 94 144 123 173 6.27e-18 75.9
Msa1303480 MtrunA17_Chr8g0338031 59.615 52 21 0 93 144 435 486 8.44e-15 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1303480 AT1G55160.2 43.846 130 63 2 17 146 18 137 5.26e-28 101
Msa1303480 AT1G55160.1 43.846 130 63 2 17 146 69 188 1.67e-27 101
Msa1303480 AT2G19530.1 36.022 186 76 5 1 146 1 183 5.26e-25 95.1
Msa1303480 AT1G55160.3 37.931 145 75 3 17 146 69 213 1.95e-22 89.0

Find 57 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGCTTAATATCTATGTTTA+AGG 0.141011 8_3:-66639738 None:intergenic
CCAAGAAATCTTCGTAATTT+AGG 0.160954 8_3:-66639301 None:intergenic
AAAGTTTCAATCTGATAAAT+AGG 0.234106 8_3:+66639551 Msa1303480:three_prime_UTR
CCTTCACCTGCACCTCCAAC+TGG 0.257972 8_3:+66637591 Msa1303480:CDS
CCTAAATTACGAAGATTTCT+TGG 0.289145 8_3:+66639301 Msa1303480:CDS
CGTTGTTGTTCGTCAGGAAT+TGG 0.294229 8_3:-66639356 None:intergenic
ACAGGACAGTAACGGGTTAA+TGG 0.339171 8_3:+66639623 Msa1303480:three_prime_UTR
ACTGAACTTGTGCTGGGTTC+AGG 0.350977 8_3:-66639275 None:intergenic
TTGCATTATTAATAACTGAT+AGG 0.355557 8_3:+66639526 Msa1303480:three_prime_UTR
CTGACCTCCAAGTGCAAGAT+TGG 0.362880 8_3:-66637661 None:intergenic
CTTTCCAATCTTGCACTTGG+AGG 0.378838 8_3:+66637657 Msa1303480:CDS
AACGAATTTGAAGCGTTTCA+TGG 0.395000 8_3:-66637619 None:intergenic
TAGGTCCACTGAACTTGTGC+TGG 0.400803 8_3:-66639282 None:intergenic
TTGCTTTCCAATCTTGCACT+TGG 0.407455 8_3:+66637654 Msa1303480:CDS
ACTTCACGTTGTTGTTCGTC+AGG 0.414100 8_3:-66639362 None:intergenic
CCATACCAATCGTGCATTGT+TGG 0.426333 8_3:+66639705 Msa1303480:three_prime_UTR
TCCCTAAAGGCTAAAACTGT+TGG 0.435819 8_3:-66639656 None:intergenic
TTGCAGAACAGGACAGTAAC+GGG 0.439281 8_3:+66639616 Msa1303480:three_prime_UTR
TGAAACGCTTCAAATTCGTT+TGG 0.442328 8_3:+66637622 Msa1303480:CDS
ATATCTGTTGGTTGTATGCC+TGG 0.449142 8_3:+66639874 Msa1303480:three_prime_UTR
GACCTACCAAGCGGGCATAA+TGG 0.466729 8_3:-66639805 None:intergenic
AGTTATGCTTGCTTCCCTAA+AGG 0.466852 8_3:-66639669 None:intergenic
GGTTCCTGCCACAATGCCTA+TGG 0.483180 8_3:+66639322 Msa1303480:CDS
AGGTCCACTGAACTTGTGCT+GGG 0.487155 8_3:-66639281 None:intergenic
GTCAAGAGGAAGTTAAAACT+TGG 0.487763 8_3:-66639501 None:intergenic
ACGAATTTGAAGCGTTTCAT+GGG 0.492853 8_3:-66637618 None:intergenic
GGTAACCCAGTTGGAGGTGC+AGG 0.493808 8_3:-66637597 None:intergenic
CACAATGCCTATGGAAGTGC+GGG 0.493963 8_3:+66639331 Msa1303480:CDS
TGCAAAACGGAGAGATTCAA+TGG 0.496763 8_3:+66639190 Msa1303480:CDS
TCTTCATGTGATATATCTGT+TGG 0.497217 8_3:+66639862 Msa1303480:three_prime_UTR
CCAACAATGCACGATTGGTA+TGG 0.503864 8_3:-66639705 None:intergenic
AGGAAGTTAAAACTTGGGTA+TGG 0.515988 8_3:-66639495 None:intergenic
TTCTGTTTGCAAGTGCAAAA+CGG 0.520014 8_3:+66639177 Msa1303480:CDS
ATTGGAGCCCGCACTTCCAT+AGG 0.522709 8_3:-66639338 None:intergenic
ATAACGATGATGATGATCAC+TGG 0.533190 8_3:-66637543 None:intergenic
TTCATGGGTAACCCAGTTGG+AGG 0.533424 8_3:-66637603 None:intergenic
CGTTTCATGGGTAACCCAGT+TGG 0.537789 8_3:-66637606 None:intergenic
TGAGAACCATTATGCCCGCT+TGG 0.543296 8_3:+66639799 Msa1303480:three_prime_UTR
GTTATCCAACAATGCACGAT+TGG 0.544948 8_3:-66639710 None:intergenic
GTTGGAGGTGCAGGTGAAGG+AGG 0.550866 8_3:-66637588 None:intergenic
CTTCACCTGCACCTCCAACT+GGG 0.560809 8_3:+66637592 Msa1303480:CDS
CCAGTTGGAGGTGCAGGTGA+AGG 0.567865 8_3:-66637591 None:intergenic
AGTGAAACCAAAGGATGCTA+CGG 0.578798 8_3:+66639415 Msa1303480:CDS
TTTGCAGAACAGGACAGTAA+CGG 0.579303 8_3:+66639615 Msa1303480:three_prime_UTR
ACTTCCATAGGCATTGTGGC+AGG 0.581843 8_3:-66639326 None:intergenic
AACCATTATGCCCGCTTGGT+AGG 0.589286 8_3:+66639803 Msa1303480:three_prime_UTR
TGAACCCAGCACAAGTTCAG+TGG 0.591880 8_3:+66639277 Msa1303480:CDS
CCACAATGCCTATGGAAGTG+CGG 0.595839 8_3:+66639330 Msa1303480:CDS
TCAAGAGGAAGTTAAAACTT+GGG 0.599682 8_3:-66639500 None:intergenic
TTAATAATGCAAGAGTCAAG+AGG 0.603817 8_3:-66639515 None:intergenic
AAAGTAGAGACCTACCAAGC+GGG 0.606497 8_3:-66639813 None:intergenic
TAACGATGATGATGATCACT+GGG 0.607328 8_3:-66637542 None:intergenic
GAACTTGTGCTGGGTTCAGG+AGG 0.607480 8_3:-66639272 None:intergenic
CCGCACTTCCATAGGCATTG+TGG 0.608838 8_3:-66639330 None:intergenic
GAAAAGAAAAGTGAAACCAA+AGG 0.630528 8_3:+66639406 Msa1303480:CDS
TGGGTATGGAATTTGCACGC+AGG 0.712652 8_3:-66639481 None:intergenic
AAAAGTAGAGACCTACCAAG+CGG 0.734554 8_3:-66639814 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATTTAATAAAATATTA+TGG + chr8_3:66638742-66638761 Msa1303480:intron 0.0%
!!! AAATATTAACAAATTTTGAT+TGG + chr8_3:66639041-66639060 Msa1303480:intron 10.0%
!!! AATATTAACAAATTTTGATT+GGG + chr8_3:66639042-66639061 Msa1303480:intron 10.0%
!! TATTTCTTATAATTGATTGA+TGG + chr8_3:66637975-66637994 Msa1303480:intron 15.0%
!!! AACTTTTTATCAGTTTAAAA+AGG + chr8_3:66638639-66638658 Msa1303480:intron 15.0%
!!! ACTTTTTATCAGTTTAAAAA+GGG + chr8_3:66638640-66638659 Msa1303480:intron 15.0%
!!! ATATTATTTGAAATCCTGAA+TGG - chr8_3:66637901-66637920 None:intergenic 20.0%
!!! GTTTTTTTTTTTCCTTTTAC+CGG - chr8_3:66638006-66638025 None:intergenic 20.0%
!! TCATATTTACTAAAGACAAT+CGG - chr8_3:66638033-66638052 None:intergenic 20.0%
!!! AAGGAAATTTTTGAATTCAA+AGG + chr8_3:66638118-66638137 Msa1303480:intron 20.0%
!!! CAATGATTTTATTGCAAAAT+TGG + chr8_3:66638229-66638248 Msa1303480:intron 20.0%
!!! TGTACGTTTTGTATTTTAAA+AGG + chr8_3:66638367-66638386 Msa1303480:intron 20.0%
!!! GTATTTTAAAAGGAAACTTT+AGG + chr8_3:66638377-66638396 Msa1303480:intron 20.0%
!! TATATAATATCTCACTAAGT+AGG - chr8_3:66638792-66638811 None:intergenic 20.0%
!! ACAAAACATTTATGCATAAA+AGG + chr8_3:66638814-66638833 Msa1303480:intron 20.0%
!!! CTTTTTGATGTTAAGAAAAA+AGG + chr8_3:66638861-66638880 Msa1303480:intron 20.0%
!! GTTAATATTTGAAAAGAGAT+AGG - chr8_3:66639031-66639050 None:intergenic 20.0%
!! AATTAAAAACATAGAATGAG+AGG + chr8_3:66639095-66639114 Msa1303480:intron 20.0%
!! TTGCATTATTAATAACTGAT+AGG + chr8_3:66639526-66639545 Msa1303480:three_prime_UTR 20.0%
!! AAAGTTTCAATCTGATAAAT+AGG + chr8_3:66639551-66639570 Msa1303480:three_prime_UTR 20.0%
!! TAGCTTAATATCTATGTTTA+AGG - chr8_3:66639741-66639760 None:intergenic 20.0%
! TCCTTGAAACACAATAAAAA+TGG - chr8_3:66637495-66637514 None:intergenic 25.0%
!! TTTTCAATCACTGTAATTGT+TGG + chr8_3:66637746-66637765 Msa1303480:intron 25.0%
! CAATATATCAAACAGATGAT+AGG - chr8_3:66637805-66637824 None:intergenic 25.0%
! AAAGCTATAAAAGATGTGTT+AGG - chr8_3:66637828-66637847 None:intergenic 25.0%
! AGTTGGACAAAATTTCAATT+AGG - chr8_3:66638347-66638366 None:intergenic 25.0%
! AAAATACAAAACGTACAAGT+TGG - chr8_3:66638364-66638383 None:intergenic 25.0%
! ACAAAAAAACATCTTTCAAC+TGG - chr8_3:66638425-66638444 None:intergenic 25.0%
! AATATAACCGTAAGTACTAT+CGG - chr8_3:66638690-66638709 None:intergenic 25.0%
!! CCTTTTTATAGCTATTATAC+AGG - chr8_3:66639073-66639092 None:intergenic 25.0%
! CCTGTATAATAGCTATAAAA+AGG + chr8_3:66639070-66639089 Msa1303480:intron 25.0%
! GAATTATCAAATACGTATCA+TGG - chr8_3:66639150-66639169 None:intergenic 25.0%
!!! GCCATTTTTATTGTGTTTCA+AGG + chr8_3:66637491-66637510 Msa1303480:five_prime_UTR 30.0%
AAGTTGACGAGAAATAAGAA+TGG - chr8_3:66637711-66637730 None:intergenic 30.0%
TCTCGTCAACTTATGATTAA+TGG + chr8_3:66637719-66637738 Msa1303480:intron 30.0%
CTCGTCAACTTATGATTAAT+GGG + chr8_3:66637720-66637739 Msa1303480:intron 30.0%
!! TTGTCTTTGTTCGAGTTTTT+GGG + chr8_3:66638165-66638184 Msa1303480:intron 30.0%
ACATTCATTTCGTGAGAAAA+GGG - chr8_3:66638269-66638288 None:intergenic 30.0%
AACATTCATTTCGTGAGAAA+AGG - chr8_3:66638270-66638289 None:intergenic 30.0%
! ATCGGAGAATTTGTACTTTT+TGG + chr8_3:66638316-66638335 Msa1303480:intron 30.0%
CAACAATGTAGTTTAGTGAT+TGG + chr8_3:66638474-66638493 Msa1303480:intron 30.0%
! AACCGTATCAACTCTTTTAA+GGG - chr8_3:66638606-66638625 None:intergenic 30.0%
! AAACCGTATCAACTCTTTTA+AGG - chr8_3:66638607-66638626 None:intergenic 30.0%
AATATCTCACTAAGTAGGAT+TGG - chr8_3:66638787-66638806 None:intergenic 30.0%
TAACATCAAAAAGCCTAGAA+GGG - chr8_3:66638854-66638873 None:intergenic 30.0%
TTAACATCAAAAAGCCTAGA+AGG - chr8_3:66638855-66638874 None:intergenic 30.0%
!! GTTAAGAAAAAAGGTGTCAA+TGG + chr8_3:66638870-66638889 Msa1303480:intron 30.0%
TACAAAAGATTTACCAAGAG+AGG - chr8_3:66638972-66638991 None:intergenic 30.0%
TTATAGCTATTATACAGGCT+TGG - chr8_3:66639068-66639087 None:intergenic 30.0%
CCAAGAAATCTTCGTAATTT+AGG - chr8_3:66639304-66639323 None:intergenic 30.0%
CCTAAATTACGAAGATTTCT+TGG + chr8_3:66639301-66639320 Msa1303480:CDS 30.0%
GAAAAGAAAAGTGAAACCAA+AGG + chr8_3:66639406-66639425 Msa1303480:CDS 30.0%
TCAAGAGGAAGTTAAAACTT+GGG - chr8_3:66639503-66639522 None:intergenic 30.0%
TTAATAATGCAAGAGTCAAG+AGG - chr8_3:66639518-66639537 None:intergenic 30.0%
!!! GTTCATTTTTTTTGCAGAAC+AGG + chr8_3:66639605-66639624 Msa1303480:three_prime_UTR 30.0%
TCTTCATGTGATATATCTGT+TGG + chr8_3:66639862-66639881 Msa1303480:three_prime_UTR 30.0%
AAGCTCGAAAAAACAGAGAA+AGG + chr8_3:66637440-66637459 Msa1303480:five_prime_UTR 35.0%
AGCTCGAAAAAACAGAGAAA+GGG + chr8_3:66637441-66637460 Msa1303480:five_prime_UTR 35.0%
! TAACGATGATGATGATCACT+GGG - chr8_3:66637545-66637564 None:intergenic 35.0%
! ATAACGATGATGATGATCAC+TGG - chr8_3:66637546-66637565 None:intergenic 35.0%
! ACGAATTTGAAGCGTTTCAT+GGG - chr8_3:66637621-66637640 None:intergenic 35.0%
! AACGAATTTGAAGCGTTTCA+TGG - chr8_3:66637622-66637641 None:intergenic 35.0%
TGAAACGCTTCAAATTCGTT+TGG + chr8_3:66637622-66637641 Msa1303480:CDS 35.0%
ACGAGAAATAAGAATGGAGA+TGG - chr8_3:66637705-66637724 None:intergenic 35.0%
AACTTTGCAAGAAACCATTC+AGG + chr8_3:66637884-66637903 Msa1303480:intron 35.0%
! TAATTGATTGATGGTTGTGC+CGG + chr8_3:66637984-66638003 Msa1303480:intron 35.0%
! AGTGTTTCTGTCACATCATT+TGG + chr8_3:66638086-66638105 Msa1303480:intron 35.0%
CATCATTTGGCATTCAAGAA+AGG + chr8_3:66638099-66638118 Msa1303480:intron 35.0%
AAGGTGATGCAGTTGAATTA+TGG + chr8_3:66638137-66638156 Msa1303480:intron 35.0%
!! CTTGTCTTTGTTCGAGTTTT+TGG + chr8_3:66638164-66638183 Msa1303480:intron 35.0%
!! TGTCTTTGTTCGAGTTTTTG+GGG + chr8_3:66638166-66638185 Msa1303480:intron 35.0%
! TCTTTCAACTGGCTTAACAA+CGG - chr8_3:66638414-66638433 None:intergenic 35.0%
GGGACAAAATCATCAGTAAT+TGG - chr8_3:66638586-66638605 None:intergenic 35.0%
!! GTCCCTTAAAAGAGTTGATA+CGG + chr8_3:66638601-66638620 Msa1303480:intron 35.0%
AGTTTGACCGATAGTACTTA+CGG + chr8_3:66638680-66638699 Msa1303480:intron 35.0%
ACCTACAATATACTGATGTG+TGG + chr8_3:66638938-66638957 Msa1303480:intron 35.0%
CCTACAATATACTGATGTGT+GGG + chr8_3:66638939-66638958 Msa1303480:intron 35.0%
AATATACTGATGTGTGGGAA+TGG + chr8_3:66638944-66638963 Msa1303480:intron 35.0%
TTCTGTTTGCAAGTGCAAAA+CGG + chr8_3:66639177-66639196 Msa1303480:CDS 35.0%
!! AACAACGTGAAGTTTTCAAG+TGG + chr8_3:66639372-66639391 Msa1303480:CDS 35.0%
!!! TGAAGTTTTCAAGTGGATTC+TGG + chr8_3:66639379-66639398 Msa1303480:CDS 35.0%
AGGAAGTTAAAACTTGGGTA+TGG - chr8_3:66639498-66639517 None:intergenic 35.0%
GTCAAGAGGAAGTTAAAACT+TGG - chr8_3:66639504-66639523 None:intergenic 35.0%
!! TTCCAACAGTTTTAGCCTTT+AGG + chr8_3:66639654-66639673 Msa1303480:three_prime_UTR 35.0%
!! TCCAACAGTTTTAGCCTTTA+GGG + chr8_3:66639655-66639674 Msa1303480:three_prime_UTR 35.0%
CAATAAAAATGGCGTGAGTG+TGG - chr8_3:66637484-66637503 None:intergenic 40.0%
TTGCTTTCCAATCTTGCACT+TGG + chr8_3:66637654-66637673 Msa1303480:CDS 40.0%
GACAAGGAAACAACACATTG+TGG - chr8_3:66637772-66637791 None:intergenic 40.0%
CGTGTCTTTATTAAGCTAGC+AGG + chr8_3:66637854-66637873 Msa1303480:intron 40.0%
ATGTAACTGCATGCTGTGAT+CGG + chr8_3:66638298-66638317 Msa1303480:intron 40.0%
! TTTCAACTGGCTTAACAACG+GGG - chr8_3:66638412-66638431 None:intergenic 40.0%
! CTTTCAACTGGCTTAACAAC+GGG - chr8_3:66638413-66638432 None:intergenic 40.0%
TAACACCATACTTTGCAGTC+TGG + chr8_3:66638512-66638531 Msa1303480:intron 40.0%
!! CTAAGTAGGATTGGCTACAT+GGG - chr8_3:66638778-66638797 None:intergenic 40.0%
!! ACTAAGTAGGATTGGCTACA+TGG - chr8_3:66638779-66638798 None:intergenic 40.0%
CCCACACATCAGTATATTGT+AGG - chr8_3:66638942-66638961 None:intergenic 40.0%
TGCAAAACGGAGAGATTCAA+TGG + chr8_3:66639190-66639209 Msa1303480:CDS 40.0%
AGTGAAACCAAAGGATGCTA+CGG + chr8_3:66639415-66639434 Msa1303480:CDS 40.0%
! TTTTTCTCCGTAGCATCCTT+TGG - chr8_3:66639425-66639444 None:intergenic 40.0%
TTTGCAGAACAGGACAGTAA+CGG + chr8_3:66639615-66639634 Msa1303480:three_prime_UTR 40.0%
TCCCTAAAGGCTAAAACTGT+TGG - chr8_3:66639659-66639678 None:intergenic 40.0%
AGTTATGCTTGCTTCCCTAA+AGG - chr8_3:66639672-66639691 None:intergenic 40.0%
GTTATCCAACAATGCACGAT+TGG - chr8_3:66639713-66639732 None:intergenic 40.0%
AAAAGTAGAGACCTACCAAG+CGG - chr8_3:66639817-66639836 None:intergenic 40.0%
CTTTCCAATCTTGCACTTGG+AGG + chr8_3:66637657-66637676 Msa1303480:CDS 45.0%
GATAGGCAAAATGCGTGACA+AGG - chr8_3:66637788-66637807 None:intergenic 45.0%
! TTGATGGTTGTGCCGGTAAA+AGG + chr8_3:66637991-66638010 Msa1303480:intron 45.0%
!! TGTTCGAGTTTTTGGGGACA+TGG + chr8_3:66638172-66638191 Msa1303480:intron 45.0%
!! GTTCGAGTTTTTGGGGACAT+GGG + chr8_3:66638173-66638192 Msa1303480:intron 45.0%
! TTCAACTGGCTTAACAACGG+GGG - chr8_3:66638411-66638430 None:intergenic 45.0%
ACCATACTTTGCAGTCTGGT+GGG + chr8_3:66638516-66638535 Msa1303480:intron 45.0%
GTAGAGACAGTGACTTCACA+TGG - chr8_3:66638564-66638583 None:intergenic 45.0%
CTAGAAGGGTTGAGAAGTTG+AGG - chr8_3:66638840-66638859 None:intergenic 45.0%
CTCAACTTCTCAACCCTTCT+AGG + chr8_3:66638838-66638857 Msa1303480:intron 45.0%
! CGTTGTTGTTCGTCAGGAAT+TGG - chr8_3:66639359-66639378 None:intergenic 45.0%
! ACTTCACGTTGTTGTTCGTC+AGG - chr8_3:66639365-66639384 None:intergenic 45.0%
TTGCAGAACAGGACAGTAAC+GGG + chr8_3:66639616-66639635 Msa1303480:three_prime_UTR 45.0%
!! ACAGGACAGTAACGGGTTAA+TGG + chr8_3:66639623-66639642 Msa1303480:three_prime_UTR 45.0%
CCAACAATGCACGATTGGTA+TGG - chr8_3:66639708-66639727 None:intergenic 45.0%
CCATACCAATCGTGCATTGT+TGG + chr8_3:66639705-66639724 Msa1303480:three_prime_UTR 45.0%
AAAGTAGAGACCTACCAAGC+GGG - chr8_3:66639816-66639835 None:intergenic 45.0%
TTCATGGGTAACCCAGTTGG+AGG - chr8_3:66637606-66637625 None:intergenic 50.0%
CGTTTCATGGGTAACCCAGT+TGG - chr8_3:66637609-66637628 None:intergenic 50.0%
CTGACCTCCAAGTGCAAGAT+TGG - chr8_3:66637664-66637683 None:intergenic 50.0%
!! GAGTTTTTGGGGACATGGGA+AGG + chr8_3:66638177-66638196 Msa1303480:intron 50.0%
!! GACATGGGAAGGAGCATCTA+CGG + chr8_3:66638188-66638207 Msa1303480:intron 50.0%
!! ACATGGGAAGGAGCATCTAC+GGG + chr8_3:66638189-66638208 Msa1303480:intron 50.0%
CACCATACTTTGCAGTCTGG+TGG + chr8_3:66638515-66638534 Msa1303480:intron 50.0%
CCATACTTTGCAGTCTGGTG+GGG + chr8_3:66638517-66638536 Msa1303480:intron 50.0%
TGTGGGAATGGTTCCTCTCT+TGG + chr8_3:66638956-66638975 Msa1303480:intron 50.0%
ACTGAACTTGTGCTGGGTTC+AGG - chr8_3:66639278-66639297 None:intergenic 50.0%
TGAACCCAGCACAAGTTCAG+TGG + chr8_3:66639277-66639296 Msa1303480:CDS 50.0%
AGGTCCACTGAACTTGTGCT+GGG - chr8_3:66639284-66639303 None:intergenic 50.0%
TAGGTCCACTGAACTTGTGC+TGG - chr8_3:66639285-66639304 None:intergenic 50.0%
! ACTTCCATAGGCATTGTGGC+AGG - chr8_3:66639329-66639348 None:intergenic 50.0%
CCACAATGCCTATGGAAGTG+CGG + chr8_3:66639330-66639349 Msa1303480:CDS 50.0%
! CACAATGCCTATGGAAGTGC+GGG + chr8_3:66639331-66639350 Msa1303480:CDS 50.0%
TGGGTATGGAATTTGCACGC+AGG - chr8_3:66639484-66639503 None:intergenic 50.0%
TGAGAACCATTATGCCCGCT+TGG + chr8_3:66639799-66639818 Msa1303480:three_prime_UTR 50.0%
AACCATTATGCCCGCTTGGT+AGG + chr8_3:66639803-66639822 Msa1303480:three_prime_UTR 50.0%
CTTCACCTGCACCTCCAACT+GGG + chr8_3:66637592-66637611 Msa1303480:CDS 55.0%
CCCCACCAGACTGCAAAGTA+TGG - chr8_3:66638520-66638539 None:intergenic 55.0%
GAACTTGTGCTGGGTTCAGG+AGG - chr8_3:66639275-66639294 None:intergenic 55.0%
GGTTCCTGCCACAATGCCTA+TGG + chr8_3:66639322-66639341 Msa1303480:CDS 55.0%
! CCGCACTTCCATAGGCATTG+TGG - chr8_3:66639333-66639352 None:intergenic 55.0%
ATTGGAGCCCGCACTTCCAT+AGG - chr8_3:66639341-66639360 None:intergenic 55.0%
GACCTACCAAGCGGGCATAA+TGG - chr8_3:66639808-66639827 None:intergenic 55.0%
! GTTGGAGGTGCAGGTGAAGG+AGG - chr8_3:66637591-66637610 None:intergenic 60.0%
! CCAGTTGGAGGTGCAGGTGA+AGG - chr8_3:66637594-66637613 None:intergenic 60.0%
CCTTCACCTGCACCTCCAAC+TGG + chr8_3:66637591-66637610 Msa1303480:CDS 60.0%
! GGTAACCCAGTTGGAGGTGC+AGG - chr8_3:66637600-66637619 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 66637435 66639896 66637435 ID=Msa1303480;Name=Msa1303480
chr8_3 mRNA 66637435 66639896 66637435 ID=Msa1303480-mRNA-1;Parent=Msa1303480;Name=Msa1303480-mRNA-1;_AED=0.00;_eAED=0.00;_QI=141|1|1|1|1|1|2|385|146
chr8_3 exon 66637435 66637678 66637435 ID=Msa1303480-mRNA-1:exon:20216;Parent=Msa1303480-mRNA-1
chr8_3 exon 66639174 66639896 66639174 ID=Msa1303480-mRNA-1:exon:20217;Parent=Msa1303480-mRNA-1
chr8_3 five_prime_UTR 66637435 66637575 66637435 ID=Msa1303480-mRNA-1:five_prime_utr;Parent=Msa1303480-mRNA-1
chr8_3 CDS 66637576 66637678 66637576 ID=Msa1303480-mRNA-1:cds;Parent=Msa1303480-mRNA-1
chr8_3 CDS 66639174 66639511 66639174 ID=Msa1303480-mRNA-1:cds;Parent=Msa1303480-mRNA-1
chr8_3 three_prime_UTR 66639512 66639896 66639512 ID=Msa1303480-mRNA-1:three_prime_utr;Parent=Msa1303480-mRNA-1
Gene Sequence

>Msa1303480

ATGAATGAGACTCCTCCTTCACCTGCACCTCCAACTGGGTTACCCATGAAACGCTTCAAATTCGTTTGGCGCTTTCTCTTGCTTTCCAATCTTGCACTTGGAGCTTTTCTGTTTGCAAGTGCAAAACGGAGAGATTCAATGGAGATTCAGAAAAAAAGAACAGCACACAGATTGCATAAGAGCAAAGCTGCTGTCGAAGTTCCTCCTGAACCCAGCACAAGTTCAGTGGACCTAAATTACGAAGATTTCTTGGTTCCTGCCACAATGCCTATGGAAGTGCGGGCTCCAATTCCTGACGAACAACAACGTGAAGTTTTCAAGTGGATTCTGGAAGAGAAAAGAAAAGTGAAACCAAAGGATGCTACGGAGAAAAAACAAATTGACGAAGACAAGTACATTCTTAAGCAGTTCCTGCGTGCAAATTCCATACCCAAGTTTTAA
Protein sequence

>Msa1303480

MNETPPSPAPPTGLPMKRFKFVWRFLLLSNLALGAFLFASAKRRDSMEIQKKRTAHRLHKSKAAVEVPPEPSTSSVDLNYEDFLVPATMPMEVRAPIPDEQQREVFKWILEEKRKVKPKDATEKKQIDEDKYILKQFLRANSIPKF*