AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1220380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1220380 MtrunA17_Chr4g0071311 96.599 147 4 1 1 146 1 147 4.77e-99 281
Msa1220380 MtrunA17_Chr8g0341971 40.441 136 58 4 16 144 62 181 2.33e-20 82.4
Msa1220380 MtrunA17_Chr8g0341991 66.667 51 17 0 94 144 123 173 6.27e-18 75.9
Msa1220380 MtrunA17_Chr8g0338031 59.615 52 21 0 93 144 435 486 8.44e-15 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1220380 AT1G55160.2 43.846 130 63 2 17 146 18 137 5.26e-28 101
Msa1220380 AT1G55160.1 43.846 130 63 2 17 146 69 188 1.67e-27 101
Msa1220380 AT2G19530.1 36.022 186 76 5 1 146 1 183 5.26e-25 95.1
Msa1220380 AT1G55160.3 37.931 145 75 3 17 146 69 213 1.95e-22 89.0

Find 58 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGCTTAATATCTATGTTTA+AGG 0.141011 8_1:+83525834 None:intergenic
CCAAGAAATCTTCGTAATTT+AGG 0.160954 8_1:+83526281 None:intergenic
AAAGTTTCAATCTGATAAAT+AGG 0.234106 8_1:-83526031 Msa1220380:three_prime_UTR
CCTTCACCTGCACCTCCAAC+TGG 0.257972 8_1:-83527991 Msa1220380:CDS
CCTAAATTACGAAGATTTCT+TGG 0.289145 8_1:-83526281 Msa1220380:CDS
CGTTGTTGTTCGTCAGGAAT+TGG 0.294229 8_1:+83526226 None:intergenic
ACAGGACAGTAACGGGTTAA+TGG 0.339171 8_1:-83525959 Msa1220380:three_prime_UTR
ACTGAACTTGTGCTGGGTTC+AGG 0.350977 8_1:+83526307 None:intergenic
TTGCATTATTAATAACTGAT+AGG 0.355557 8_1:-83526056 Msa1220380:three_prime_UTR
CTGACCTCCAAGTGCAAGAT+TGG 0.362880 8_1:+83527921 None:intergenic
CTTTCCAATCTTGCACTTGG+AGG 0.378838 8_1:-83527925 Msa1220380:intron
AACGAATTTGAAGCGTTTCA+TGG 0.395000 8_1:+83527963 None:intergenic
TAGGTCCACTGAACTTGTGC+TGG 0.400803 8_1:+83526300 None:intergenic
TTGCTTTCCAATCTTGCACT+TGG 0.407455 8_1:-83527928 Msa1220380:CDS
ACTTCACGTTGTTGTTCGTC+AGG 0.414100 8_1:+83526220 None:intergenic
CCATACCAATCGTGCATTGT+TGG 0.426333 8_1:-83525867 Msa1220380:three_prime_UTR
ATGGTTACAAGAATGTAGTT+AGG 0.427854 8_1:+83525886 None:intergenic
TCCCTAAAGGCTAAAACTGT+TGG 0.435819 8_1:+83525926 None:intergenic
TTGCAGAACAGGACAGTAAC+GGG 0.439281 8_1:-83525966 Msa1220380:three_prime_UTR
TGAAACGCTTCAAATTCGTT+TGG 0.442328 8_1:-83527960 Msa1220380:CDS
ATATCTGTTGGTTGTATGCC+TGG 0.449142 8_1:-83525698 Msa1220380:three_prime_UTR
GACCTACCAAGCGGGCATAA+TGG 0.466729 8_1:+83525767 None:intergenic
TTTGATGCTTGCTTCCCTAA+AGG 0.470226 8_1:+83525913 None:intergenic
GGTTCCTGCCACAATGCCTA+TGG 0.483180 8_1:-83526260 Msa1220380:CDS
AGGTCCACTGAACTTGTGCT+GGG 0.487155 8_1:+83526301 None:intergenic
GTCAAGAGGAAGTTAAAACT+TGG 0.487763 8_1:+83526081 None:intergenic
ACGAATTTGAAGCGTTTCAT+GGG 0.492853 8_1:+83527964 None:intergenic
GGTAACCCAGTTGGAGGTGC+AGG 0.493808 8_1:+83527985 None:intergenic
CACAATGCCTATGGAAGTGC+GGG 0.493963 8_1:-83526251 Msa1220380:CDS
TGCAAAACGGAGAGATTCAA+TGG 0.496763 8_1:-83526392 Msa1220380:CDS
TCTTCATGTGATATATCTGT+TGG 0.497217 8_1:-83525710 Msa1220380:three_prime_UTR
CCAACAATGCACGATTGGTA+TGG 0.503864 8_1:+83525867 None:intergenic
AGGAAGTTAAAACTTGGGTA+TGG 0.515988 8_1:+83526087 None:intergenic
TTCTGTTTGCAAGTGCAAAA+CGG 0.520014 8_1:-83526405 Msa1220380:CDS
ATTGGAGCCCGCACTTCCAT+AGG 0.522709 8_1:+83526244 None:intergenic
ATAACGATGATGATGATCAC+TGG 0.533190 8_1:+83528039 None:intergenic
TTCATGGGTAACCCAGTTGG+AGG 0.533424 8_1:+83527979 None:intergenic
CGTTTCATGGGTAACCCAGT+TGG 0.537789 8_1:+83527976 None:intergenic
TGAGAACCATTATGCCCGCT+TGG 0.543296 8_1:-83525773 Msa1220380:three_prime_UTR
GTTATCCAACAATGCACGAT+TGG 0.544948 8_1:+83525862 None:intergenic
GTTGGAGGTGCAGGTGAAGG+AGG 0.550866 8_1:+83527994 None:intergenic
CTTCACCTGCACCTCCAACT+GGG 0.560809 8_1:-83527990 Msa1220380:CDS
CCAGTTGGAGGTGCAGGTGA+AGG 0.567865 8_1:+83527991 None:intergenic
AGTGAAACCAAAGGATGCTA+CGG 0.578798 8_1:-83526167 Msa1220380:CDS
TTTGCAGAACAGGACAGTAA+CGG 0.579303 8_1:-83525967 Msa1220380:three_prime_UTR
ACTTCCATAGGCATTGTGGC+AGG 0.581843 8_1:+83526256 None:intergenic
AACCATTATGCCCGCTTGGT+AGG 0.589286 8_1:-83525769 Msa1220380:three_prime_UTR
TGAACCCAGCACAAGTTCAG+TGG 0.591880 8_1:-83526305 Msa1220380:CDS
CCACAATGCCTATGGAAGTG+CGG 0.595839 8_1:-83526252 Msa1220380:CDS
TCAAGAGGAAGTTAAAACTT+GGG 0.599682 8_1:+83526082 None:intergenic
TTAATAATGCAAGAGTCAAG+AGG 0.603817 8_1:+83526067 None:intergenic
AAAGTAGAGACCTACCAAGC+GGG 0.606497 8_1:+83525759 None:intergenic
TAACGATGATGATGATCACT+GGG 0.607328 8_1:+83528040 None:intergenic
GAACTTGTGCTGGGTTCAGG+AGG 0.607480 8_1:+83526310 None:intergenic
CCGCACTTCCATAGGCATTG+TGG 0.608838 8_1:+83526252 None:intergenic
GAAAAGAAAAGTGAAACCAA+AGG 0.630528 8_1:-83526176 Msa1220380:CDS
TGGGTATGGAATTTGCACGC+AGG 0.712652 8_1:+83526101 None:intergenic
AAAAGTAGAGACCTACCAAG+CGG 0.734554 8_1:+83525758 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATTTAATAAAATATTA+TGG - chr8_1:83527005-83527024 Msa1220380:intron 0.0%
!!! AAATATTAACAAATTTTGAT+TGG - chr8_1:83527304-83527323 Msa1220380:intron 10.0%
!!! AATATTAACAAATTTTGATT+GGG - chr8_1:83527305-83527324 Msa1220380:intron 10.0%
!! TATTTCTTATAATTGATTGA+TGG - chr8_1:83526238-83526257 Msa1220380:CDS 15.0%
!!! AACTTTTTATCAGTTTAAAA+AGG - chr8_1:83526902-83526921 Msa1220380:intron 15.0%
!!! ACTTTTTATCAGTTTAAAAA+GGG - chr8_1:83526903-83526922 Msa1220380:intron 15.0%
!!! ATATTATTTGAAATCCTGAA+TGG + chr8_1:83526164-83526183 None:intergenic 20.0%
!!! GTTTTTTTTTTTCCTTTTAC+CGG + chr8_1:83526269-83526288 None:intergenic 20.0%
!! TCATATTTACTAAAGACAAT+CGG + chr8_1:83526296-83526315 None:intergenic 20.0%
!! TAAATATGACTATGTTATGA+TGG - chr8_1:83526307-83526326 Msa1220380:CDS 20.0%
!!! AAGGAAATTTTTGAATTCAA+AGG - chr8_1:83526381-83526400 Msa1220380:CDS 20.0%
!!! CAATGATTTTATTGCAAAAT+TGG - chr8_1:83526492-83526511 Msa1220380:intron 20.0%
!!! TGTACGTTTTGTATTTTAAA+AGG - chr8_1:83526630-83526649 Msa1220380:intron 20.0%
!!! GTATTTTAAAAGGAAACTTT+AGG - chr8_1:83526640-83526659 Msa1220380:intron 20.0%
!! TATATAATATCTCACTAAGT+AGG + chr8_1:83527055-83527074 None:intergenic 20.0%
!! ACAAAACATTTATGCATAAA+AGG - chr8_1:83527077-83527096 Msa1220380:intron 20.0%
!!! CTTTTTGATGTTAAGAAAAA+AGG - chr8_1:83527124-83527143 Msa1220380:intron 20.0%
!! GTTAATATTTGAAAAGAGAT+AGG + chr8_1:83527294-83527313 None:intergenic 20.0%
!! AATTAAAAACATAGAATGAG+AGG - chr8_1:83527358-83527377 Msa1220380:intron 20.0%
!! TTGCATTATTAATAACTGAT+AGG - chr8_1:83527789-83527808 Msa1220380:intron 20.0%
!! AAAGTTTCAATCTGATAAAT+AGG - chr8_1:83527814-83527833 Msa1220380:intron 20.0%
!! TAGCTTAATATCTATGTTTA+AGG + chr8_1:83528014-83528033 None:intergenic 20.0%
! TCCTTGAAACACAATAAAAA+TGG + chr8_1:83525758-83525777 None:intergenic 25.0%
!! TTTTCAATCACTGTAATTGT+TGG - chr8_1:83526009-83526028 Msa1220380:three_prime_UTR 25.0%
! CAATATATCAAACAGATGAT+AGG + chr8_1:83526068-83526087 None:intergenic 25.0%
! AAAGCTATAAAAGATGTGTT+AGG + chr8_1:83526091-83526110 None:intergenic 25.0%
! AGTTGGACAAAATTTCAATT+AGG + chr8_1:83526610-83526629 None:intergenic 25.0%
! AAAATACAAAACGTACAAGT+TGG + chr8_1:83526627-83526646 None:intergenic 25.0%
! ACAAAAAAACATCTTTCAAC+TGG + chr8_1:83526688-83526707 None:intergenic 25.0%
! AATATAACCGTAAGTACTAT+CGG + chr8_1:83526953-83526972 None:intergenic 25.0%
!! CCTTTTTATAGCTATTATAC+AGG + chr8_1:83527336-83527355 None:intergenic 25.0%
! CCTGTATAATAGCTATAAAA+AGG - chr8_1:83527333-83527352 Msa1220380:intron 25.0%
! GAATTATCAAATACGTATCA+TGG + chr8_1:83527413-83527432 None:intergenic 25.0%
!!! GCCATTTTTATTGTGTTTCA+AGG - chr8_1:83525754-83525773 Msa1220380:three_prime_UTR 30.0%
AAGTTGACGAGAAATAAGAA+TGG + chr8_1:83525974-83525993 None:intergenic 30.0%
TCTCGTCAACTTATGATTAA+TGG - chr8_1:83525982-83526001 Msa1220380:three_prime_UTR 30.0%
CTCGTCAACTTATGATTAAT+GGG - chr8_1:83525983-83526002 Msa1220380:three_prime_UTR 30.0%
!! TTGTCTTTGTTCGAGTTTTT+GGG - chr8_1:83526428-83526447 Msa1220380:intron 30.0%
ACATTCATTTCGTGAGAAAA+GGG + chr8_1:83526532-83526551 None:intergenic 30.0%
AACATTCATTTCGTGAGAAA+AGG + chr8_1:83526533-83526552 None:intergenic 30.0%
! ATCGGAGAATTTGTACTTTT+TGG - chr8_1:83526579-83526598 Msa1220380:intron 30.0%
CAACAATGTAGTTTAGTGAT+TGG - chr8_1:83526737-83526756 Msa1220380:intron 30.0%
! AACCGTATCAACTCTTTTAA+GGG + chr8_1:83526869-83526888 None:intergenic 30.0%
! AAACCGTATCAACTCTTTTA+AGG + chr8_1:83526870-83526889 None:intergenic 30.0%
AATATCTCACTAAGTAGGAT+TGG + chr8_1:83527050-83527069 None:intergenic 30.0%
TAACATCAAAAAGCCTAGAA+GGG + chr8_1:83527117-83527136 None:intergenic 30.0%
TTAACATCAAAAAGCCTAGA+AGG + chr8_1:83527118-83527137 None:intergenic 30.0%
!! GTTAAGAAAAAAGGTGTCAA+TGG - chr8_1:83527133-83527152 Msa1220380:intron 30.0%
TACAAAAGATTTACCAAGAG+AGG + chr8_1:83527235-83527254 None:intergenic 30.0%
TTATAGCTATTATACAGGCT+TGG + chr8_1:83527331-83527350 None:intergenic 30.0%
CCAAGAAATCTTCGTAATTT+AGG + chr8_1:83527567-83527586 None:intergenic 30.0%
CCTAAATTACGAAGATTTCT+TGG - chr8_1:83527564-83527583 Msa1220380:intron 30.0%
GAAAAGAAAAGTGAAACCAA+AGG - chr8_1:83527669-83527688 Msa1220380:intron 30.0%
TCAAGAGGAAGTTAAAACTT+GGG + chr8_1:83527766-83527785 None:intergenic 30.0%
TTAATAATGCAAGAGTCAAG+AGG + chr8_1:83527781-83527800 None:intergenic 30.0%
!!! GTTCATTTTTTTTGCAGAAC+AGG - chr8_1:83527868-83527887 Msa1220380:intron 30.0%
ATGGTTACAAGAATGTAGTT+AGG + chr8_1:83527962-83527981 None:intergenic 30.0%
TCTTCATGTGATATATCTGT+TGG - chr8_1:83528135-83528154 Msa1220380:five_prime_UTR 30.0%
AAGCTCGAAAAAACAGAGAA+AGG - chr8_1:83525703-83525722 Msa1220380:three_prime_UTR 35.0%
AGCTCGAAAAAACAGAGAAA+GGG - chr8_1:83525704-83525723 Msa1220380:three_prime_UTR 35.0%
! TAACGATGATGATGATCACT+GGG + chr8_1:83525808-83525827 None:intergenic 35.0%
! ATAACGATGATGATGATCAC+TGG + chr8_1:83525809-83525828 None:intergenic 35.0%
! ACGAATTTGAAGCGTTTCAT+GGG + chr8_1:83525884-83525903 None:intergenic 35.0%
! AACGAATTTGAAGCGTTTCA+TGG + chr8_1:83525885-83525904 None:intergenic 35.0%
TGAAACGCTTCAAATTCGTT+TGG - chr8_1:83525885-83525904 Msa1220380:three_prime_UTR 35.0%
ACGAGAAATAAGAATGGAGA+TGG + chr8_1:83525968-83525987 None:intergenic 35.0%
AACTTTGCAAGAAACCATTC+AGG - chr8_1:83526147-83526166 Msa1220380:CDS 35.0%
! TAATTGATTGATGGTTGTGC+CGG - chr8_1:83526247-83526266 Msa1220380:CDS 35.0%
! AGTGTTTCTGTCACATCATT+TGG - chr8_1:83526349-83526368 Msa1220380:CDS 35.0%
CATCATTTGGCATTCAAGAA+AGG - chr8_1:83526362-83526381 Msa1220380:CDS 35.0%
AAGGTGATGCAGTTGAATTA+TGG - chr8_1:83526400-83526419 Msa1220380:CDS 35.0%
!! CTTGTCTTTGTTCGAGTTTT+TGG - chr8_1:83526427-83526446 Msa1220380:intron 35.0%
!! TGTCTTTGTTCGAGTTTTTG+GGG - chr8_1:83526429-83526448 Msa1220380:intron 35.0%
! TCTTTCAACTGGCTTAACAA+CGG + chr8_1:83526677-83526696 None:intergenic 35.0%
GGGACAAAATCATCAGTAAT+TGG + chr8_1:83526849-83526868 None:intergenic 35.0%
!! GTCCCTTAAAAGAGTTGATA+CGG - chr8_1:83526864-83526883 Msa1220380:intron 35.0%
AGTTTGACCGATAGTACTTA+CGG - chr8_1:83526943-83526962 Msa1220380:intron 35.0%
ACCTACAATATACTGATGTG+TGG - chr8_1:83527201-83527220 Msa1220380:intron 35.0%
CCTACAATATACTGATGTGT+GGG - chr8_1:83527202-83527221 Msa1220380:intron 35.0%
AATATACTGATGTGTGGGAA+TGG - chr8_1:83527207-83527226 Msa1220380:intron 35.0%
TTCTGTTTGCAAGTGCAAAA+CGG - chr8_1:83527440-83527459 Msa1220380:intron 35.0%
!! AACAACGTGAAGTTTTCAAG+TGG - chr8_1:83527635-83527654 Msa1220380:intron 35.0%
!!! TGAAGTTTTCAAGTGGATTC+TGG - chr8_1:83527642-83527661 Msa1220380:intron 35.0%
AGGAAGTTAAAACTTGGGTA+TGG + chr8_1:83527761-83527780 None:intergenic 35.0%
GTCAAGAGGAAGTTAAAACT+TGG + chr8_1:83527767-83527786 None:intergenic 35.0%
!! TTCCAACAGTTTTAGCCTTT+AGG - chr8_1:83527917-83527936 Msa1220380:intron 35.0%
!! TCCAACAGTTTTAGCCTTTA+GGG - chr8_1:83527918-83527937 Msa1220380:intron 35.0%
CAATAAAAATGGCGTGAGTG+TGG + chr8_1:83525747-83525766 None:intergenic 40.0%
TTGCTTTCCAATCTTGCACT+TGG - chr8_1:83525917-83525936 Msa1220380:three_prime_UTR 40.0%
GACAAGGAAACAACACATTG+TGG + chr8_1:83526035-83526054 None:intergenic 40.0%
CGTGTCTTTATTAAGCTAGC+AGG - chr8_1:83526117-83526136 Msa1220380:CDS 40.0%
ATGTAACTGCATGCTGTGAT+CGG - chr8_1:83526561-83526580 Msa1220380:intron 40.0%
! TTTCAACTGGCTTAACAACG+GGG + chr8_1:83526675-83526694 None:intergenic 40.0%
! CTTTCAACTGGCTTAACAAC+GGG + chr8_1:83526676-83526695 None:intergenic 40.0%
TAACACCATACTTTGCAGTC+TGG - chr8_1:83526775-83526794 Msa1220380:intron 40.0%
!! CTAAGTAGGATTGGCTACAT+GGG + chr8_1:83527041-83527060 None:intergenic 40.0%
!! ACTAAGTAGGATTGGCTACA+TGG + chr8_1:83527042-83527061 None:intergenic 40.0%
CCCACACATCAGTATATTGT+AGG + chr8_1:83527205-83527224 None:intergenic 40.0%
TGCAAAACGGAGAGATTCAA+TGG - chr8_1:83527453-83527472 Msa1220380:intron 40.0%
AGTGAAACCAAAGGATGCTA+CGG - chr8_1:83527678-83527697 Msa1220380:intron 40.0%
! TTTTTCTCCGTAGCATCCTT+TGG + chr8_1:83527688-83527707 None:intergenic 40.0%
TTTGCAGAACAGGACAGTAA+CGG - chr8_1:83527878-83527897 Msa1220380:intron 40.0%
TCCCTAAAGGCTAAAACTGT+TGG + chr8_1:83527922-83527941 None:intergenic 40.0%
TTTGATGCTTGCTTCCCTAA+AGG + chr8_1:83527935-83527954 None:intergenic 40.0%
GTTATCCAACAATGCACGAT+TGG + chr8_1:83527986-83528005 None:intergenic 40.0%
AAAAGTAGAGACCTACCAAG+CGG + chr8_1:83528090-83528109 None:intergenic 40.0%
CTTTCCAATCTTGCACTTGG+AGG - chr8_1:83525920-83525939 Msa1220380:three_prime_UTR 45.0%
GATAGGCAAAATGCGTGACA+AGG + chr8_1:83526051-83526070 None:intergenic 45.0%
! TTGATGGTTGTGCCGGTAAA+AGG - chr8_1:83526254-83526273 Msa1220380:CDS 45.0%
!! TGTTCGAGTTTTTGGGGACA+TGG - chr8_1:83526435-83526454 Msa1220380:intron 45.0%
!! GTTCGAGTTTTTGGGGACAT+GGG - chr8_1:83526436-83526455 Msa1220380:intron 45.0%
! TTCAACTGGCTTAACAACGG+GGG + chr8_1:83526674-83526693 None:intergenic 45.0%
ACCATACTTTGCAGTCTGGT+GGG - chr8_1:83526779-83526798 Msa1220380:intron 45.0%
GTAGAGACAGTGACTTCACA+TGG + chr8_1:83526827-83526846 None:intergenic 45.0%
CTAGAAGGGTTGAGAAGTTG+AGG + chr8_1:83527103-83527122 None:intergenic 45.0%
CTCAACTTCTCAACCCTTCT+AGG - chr8_1:83527101-83527120 Msa1220380:intron 45.0%
! CGTTGTTGTTCGTCAGGAAT+TGG + chr8_1:83527622-83527641 None:intergenic 45.0%
! ACTTCACGTTGTTGTTCGTC+AGG + chr8_1:83527628-83527647 None:intergenic 45.0%
TTGCAGAACAGGACAGTAAC+GGG - chr8_1:83527879-83527898 Msa1220380:intron 45.0%
!! ACAGGACAGTAACGGGTTAA+TGG - chr8_1:83527886-83527905 Msa1220380:intron 45.0%
CCAACAATGCACGATTGGTA+TGG + chr8_1:83527981-83528000 None:intergenic 45.0%
CCATACCAATCGTGCATTGT+TGG - chr8_1:83527978-83527997 Msa1220380:CDS 45.0%
AAAGTAGAGACCTACCAAGC+GGG + chr8_1:83528089-83528108 None:intergenic 45.0%
TTCATGGGTAACCCAGTTGG+AGG + chr8_1:83525869-83525888 None:intergenic 50.0%
CGTTTCATGGGTAACCCAGT+TGG + chr8_1:83525872-83525891 None:intergenic 50.0%
CTGACCTCCAAGTGCAAGAT+TGG + chr8_1:83525927-83525946 None:intergenic 50.0%
!! GAGTTTTTGGGGACATGGGA+AGG - chr8_1:83526440-83526459 Msa1220380:intron 50.0%
!! GACATGGGAAGGAGCATCTA+CGG - chr8_1:83526451-83526470 Msa1220380:intron 50.0%
!! ACATGGGAAGGAGCATCTAC+GGG - chr8_1:83526452-83526471 Msa1220380:intron 50.0%
CACCATACTTTGCAGTCTGG+TGG - chr8_1:83526778-83526797 Msa1220380:intron 50.0%
CCATACTTTGCAGTCTGGTG+GGG - chr8_1:83526780-83526799 Msa1220380:intron 50.0%
TGTGGGAATGGTTCCTCTCT+TGG - chr8_1:83527219-83527238 Msa1220380:intron 50.0%
ACTGAACTTGTGCTGGGTTC+AGG + chr8_1:83527541-83527560 None:intergenic 50.0%
TGAACCCAGCACAAGTTCAG+TGG - chr8_1:83527540-83527559 Msa1220380:intron 50.0%
AGGTCCACTGAACTTGTGCT+GGG + chr8_1:83527547-83527566 None:intergenic 50.0%
TAGGTCCACTGAACTTGTGC+TGG + chr8_1:83527548-83527567 None:intergenic 50.0%
! ACTTCCATAGGCATTGTGGC+AGG + chr8_1:83527592-83527611 None:intergenic 50.0%
CCACAATGCCTATGGAAGTG+CGG - chr8_1:83527593-83527612 Msa1220380:intron 50.0%
! CACAATGCCTATGGAAGTGC+GGG - chr8_1:83527594-83527613 Msa1220380:intron 50.0%
TGGGTATGGAATTTGCACGC+AGG + chr8_1:83527747-83527766 None:intergenic 50.0%
TGAGAACCATTATGCCCGCT+TGG - chr8_1:83528072-83528091 Msa1220380:five_prime_UTR 50.0%
AACCATTATGCCCGCTTGGT+AGG - chr8_1:83528076-83528095 Msa1220380:five_prime_UTR 50.0%
CTTCACCTGCACCTCCAACT+GGG - chr8_1:83525855-83525874 Msa1220380:three_prime_UTR 55.0%
CCCCACCAGACTGCAAAGTA+TGG + chr8_1:83526783-83526802 None:intergenic 55.0%
GAACTTGTGCTGGGTTCAGG+AGG + chr8_1:83527538-83527557 None:intergenic 55.0%
GGTTCCTGCCACAATGCCTA+TGG - chr8_1:83527585-83527604 Msa1220380:intron 55.0%
! CCGCACTTCCATAGGCATTG+TGG + chr8_1:83527596-83527615 None:intergenic 55.0%
ATTGGAGCCCGCACTTCCAT+AGG + chr8_1:83527604-83527623 None:intergenic 55.0%
GACCTACCAAGCGGGCATAA+TGG + chr8_1:83528081-83528100 None:intergenic 55.0%
! GTTGGAGGTGCAGGTGAAGG+AGG + chr8_1:83525854-83525873 None:intergenic 60.0%
! CCAGTTGGAGGTGCAGGTGA+AGG + chr8_1:83525857-83525876 None:intergenic 60.0%
CCTTCACCTGCACCTCCAAC+TGG - chr8_1:83525854-83525873 Msa1220380:three_prime_UTR 60.0%
! GGTAACCCAGTTGGAGGTGC+AGG + chr8_1:83525863-83525882 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 83525698 83528169 83525698 ID=Msa1220380;Name=Msa1220380
chr8_1 mRNA 83525698 83528169 83525698 ID=Msa1220380-mRNA-1;Parent=Msa1220380;Name=Msa1220380-mRNA-1;_AED=0.00;_eAED=0.00;_QI=141|1|1|1|1|1|2|395|146
chr8_1 exon 83525698 83526430 83525698 ID=Msa1220380-mRNA-1:exon:29957;Parent=Msa1220380-mRNA-1
chr8_1 exon 83527926 83528169 83527926 ID=Msa1220380-mRNA-1:exon:29956;Parent=Msa1220380-mRNA-1
chr8_1 five_prime_UTR 83528029 83528169 83528029 ID=Msa1220380-mRNA-1:five_prime_utr;Parent=Msa1220380-mRNA-1
chr8_1 CDS 83527926 83528028 83527926 ID=Msa1220380-mRNA-1:cds;Parent=Msa1220380-mRNA-1
chr8_1 CDS 83526093 83526430 83526093 ID=Msa1220380-mRNA-1:cds;Parent=Msa1220380-mRNA-1
chr8_1 three_prime_UTR 83525698 83526092 83525698 ID=Msa1220380-mRNA-1:three_prime_utr;Parent=Msa1220380-mRNA-1
Gene Sequence

>Msa1220380

ATGAATGAGACTCCTCCTTCACCTGCACCTCCAACTGGGTTACCCATGAAACGCTTCAAATTCGTTTGGCGCTTTCTCTTGCTTTCCAATCTTGCACTTGGAGCTTTTCTGTTTGCAAGTGCAAAACGGAGAGATTCAATGGAGATTCAGAAAAAAAGAACAGCACACAGATTGCATAAGAGCAAAGCTGCTGTCGAAGTTCCTCCTGAACCCAGCACAAGTTCAGTGGACCTAAATTACGAAGATTTCTTGGTTCCTGCCACAATGCCTATGGAAGTGCGGGCTCCAATTCCTGACGAACAACAACGTGAAGTTTTCAAGTGGATTCTGGAAGAGAAAAGAAAAGTGAAACCAAAGGATGCTACGGAGAAAAAACAAATTGACGAAGACAAGTACATTCTTAAGCAGTTCCTGCGTGCAAATTCCATACCCAAGTTTTAA
Protein sequence

>Msa1220380

MNETPPSPAPPTGLPMKRFKFVWRFLLLSNLALGAFLFASAKRRDSMEIQKKRTAHRLHKSKAAVEVPPEPSTSSVDLNYEDFLVPATMPMEVRAPIPDEQQREVFKWILEEKRKVKPKDATEKKQIDEDKYILKQFLRANSIPKF*