AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1220280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1220280 MtrunA17_Chr4g0071311 97.279 147 4 0 1 147 1 147 3.80e-101 286
Msa1220280 MtrunA17_Chr8g0341971 39.855 138 60 4 17 147 62 183 3.85e-20 81.6
Msa1220280 MtrunA17_Chr8g0341991 66.667 51 17 0 95 145 123 173 7.19e-18 75.9
Msa1220280 MtrunA17_Chr8g0338031 59.615 52 21 0 94 145 435 486 9.67e-15 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1220280 AT1G55160.2 43.846 130 63 2 18 147 18 137 7.37e-28 100
Msa1220280 AT1G55160.1 43.846 130 63 2 18 147 69 188 1.71e-27 101
Msa1220280 AT2G19530.1 36.216 185 75 5 3 147 2 183 1.44e-24 94.0
Msa1220280 AT1G55160.3 37.931 145 75 3 18 147 69 213 2.24e-22 88.6

Find 60 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGCTTAATATCTATGTTTA+AGG 0.141011 8_1:+83340322 None:intergenic
CCAAGAAATCTTCGTAATTT+AGG 0.160954 8_1:+83340769 None:intergenic
AAAGTTTCAATCTGATAAAT+AGG 0.234106 8_1:-83340519 Msa1220280:three_prime_UTR
CCTAAATTACGAAGATTTCT+TGG 0.289145 8_1:-83340769 Msa1220280:CDS
CGTTGTTGTTCGTCAGGAAT+TGG 0.294229 8_1:+83340714 None:intergenic
GGAGGTGCAGGTGGTGGTGG+AGG 0.305380 8_1:+83342485 None:intergenic
CCACCACCTGCACCTCCAAC+TGG 0.312869 8_1:-83342479 Msa1220280:CDS
GTTGGAGGTGCAGGTGGTGG+TGG 0.337689 8_1:+83342482 None:intergenic
ACAGGACAGTAACGGGTTAA+TGG 0.339171 8_1:-83340447 Msa1220280:three_prime_UTR
ACTGAACTTGTGCTGGGTTC+AGG 0.350977 8_1:+83340795 None:intergenic
TTGCATTATTAATAACTGAT+AGG 0.355557 8_1:-83340544 Msa1220280:three_prime_UTR
CTGACCTCCAAGTGCAAGAT+TGG 0.362880 8_1:+83342409 None:intergenic
CTTTCCAATCTTGCACTTGG+AGG 0.378838 8_1:-83342413 Msa1220280:intron
AACGAATTTGAAGCGCTTCA+TGG 0.390103 8_1:+83342451 None:intergenic
TAGGTCCACTGAACTTGTGC+TGG 0.400803 8_1:+83340788 None:intergenic
TTGCTTTCCAATCTTGCACT+TGG 0.407455 8_1:-83342416 Msa1220280:CDS
ACTTCACGTTGTTGTTCGTC+AGG 0.414100 8_1:+83340708 None:intergenic
TGAAGCGCTTCAAATTCGTT+TGG 0.421156 8_1:-83342448 Msa1220280:CDS
CCATACCAATCGTGCATTGT+TGG 0.426333 8_1:-83340355 Msa1220280:three_prime_UTR
ATGGTTACAAGAATGTAGTT+AGG 0.427854 8_1:+83340374 None:intergenic
TCCCTAAAGGCTAAAACTGT+TGG 0.435819 8_1:+83340414 None:intergenic
TTGCAGAACAGGACAGTAAC+GGG 0.439281 8_1:-83340454 Msa1220280:three_prime_UTR
ATATCTGTTGGTTGTATGCC+TGG 0.449142 8_1:-83340186 Msa1220280:three_prime_UTR
GACCTACCAAGCGGGCATAA+TGG 0.466729 8_1:+83340255 None:intergenic
TTTGATGCTTGCTTCCCTAA+AGG 0.470226 8_1:+83340401 None:intergenic
CCAGTTGGAGGTGCAGGTGG+TGG 0.474108 8_1:+83342479 None:intergenic
GGTTCCTGCCACAATGCCTA+TGG 0.483180 8_1:-83340748 Msa1220280:CDS
GGTAACCCAGTTGGAGGTGC+AGG 0.486372 8_1:+83342473 None:intergenic
AGGTCCACTGAACTTGTGCT+GGG 0.487155 8_1:+83340789 None:intergenic
GTCAAGAGGAAGTTAAAACT+TGG 0.487763 8_1:+83340569 None:intergenic
ACGAATTTGAAGCGCTTCAT+GGG 0.490398 8_1:+83342452 None:intergenic
CACAATGCCTATGGAAGTGC+GGG 0.493963 8_1:-83340739 Msa1220280:CDS
TGCAAAACGGAGAGATTCAA+TGG 0.496763 8_1:-83340880 Msa1220280:CDS
TCTTCATGTGATATATCTGT+TGG 0.497217 8_1:-83340198 Msa1220280:three_prime_UTR
CCAACAATGCACGATTGGTA+TGG 0.503864 8_1:+83340355 None:intergenic
AACCCAGTTGGAGGTGCAGG+TGG 0.506178 8_1:+83342476 None:intergenic
CGCTTCATGGGTAACCCAGT+TGG 0.511657 8_1:+83342464 None:intergenic
AGGAAGTTAAAACTTGGGTA+TGG 0.515988 8_1:+83340575 None:intergenic
TTCTGTTTGCAAGTGCAAAA+CGG 0.520014 8_1:-83340893 Msa1220280:CDS
ATTGGAGCCCGCACTTCCAT+AGG 0.522709 8_1:+83340732 None:intergenic
ATAACGATGATGATGATCAC+TGG 0.533190 8_1:+83342530 None:intergenic
TGAGAACCATTATGCCCGCT+TGG 0.543296 8_1:-83340261 Msa1220280:three_prime_UTR
GTTATCCAACAATGCACGAT+TGG 0.544948 8_1:+83340350 None:intergenic
TTCATGGGTAACCCAGTTGG+AGG 0.563986 8_1:+83342467 None:intergenic
CACCACCTGCACCTCCAACT+GGG 0.568170 8_1:-83342478 Msa1220280:CDS
AGTGAAACCAAAGGATGCTA+CGG 0.578798 8_1:-83340655 Msa1220280:CDS
TTTGCAGAACAGGACAGTAA+CGG 0.579303 8_1:-83340455 Msa1220280:three_prime_UTR
ACTTCCATAGGCATTGTGGC+AGG 0.581843 8_1:+83340744 None:intergenic
AACCATTATGCCCGCTTGGT+AGG 0.589286 8_1:-83340257 Msa1220280:three_prime_UTR
TGAACCCAGCACAAGTTCAG+TGG 0.591880 8_1:-83340793 Msa1220280:CDS
CCACAATGCCTATGGAAGTG+CGG 0.595839 8_1:-83340740 Msa1220280:CDS
TCAAGAGGAAGTTAAAACTT+GGG 0.599682 8_1:+83340570 None:intergenic
TTAATAATGCAAGAGTCAAG+AGG 0.603817 8_1:+83340555 None:intergenic
AAAGTAGAGACCTACCAAGC+GGG 0.606497 8_1:+83340247 None:intergenic
GAACTTGTGCTGGGTTCAGG+AGG 0.607480 8_1:+83340798 None:intergenic
CCGCACTTCCATAGGCATTG+TGG 0.608838 8_1:+83340740 None:intergenic
TAACGATGATGATGATCACT+GGG 0.610234 8_1:+83342531 None:intergenic
GAAAAGAAAAGTGAAACCAA+AGG 0.630528 8_1:-83340664 Msa1220280:CDS
TGGGTATGGAATTTGCACGC+AGG 0.712652 8_1:+83340589 None:intergenic
AAAAGTAGAGACCTACCAAG+CGG 0.734554 8_1:+83340246 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATTTAATAAAATATTA+TGG - chr8_1:83341496-83341515 Msa1220280:intron 0.0%
!!! AAATATTAACAAATTTTGAT+TGG - chr8_1:83341795-83341814 Msa1220280:intron 10.0%
!!! AATATTAACAAATTTTGATT+GGG - chr8_1:83341796-83341815 Msa1220280:intron 10.0%
!! TATTTCTTATAATTGATTGA+TGG - chr8_1:83340729-83340748 Msa1220280:CDS 15.0%
!!! AACTTTTTATCAGTTTAAAA+AGG - chr8_1:83341393-83341412 Msa1220280:intron 15.0%
!!! ACTTTTTATCAGTTTAAAAA+GGG - chr8_1:83341394-83341413 Msa1220280:intron 15.0%
!!! ATATTATTTGAAATCCTGAA+TGG + chr8_1:83340655-83340674 None:intergenic 20.0%
!!! GTTTTTTTTTTTCCTTTTAC+CGG + chr8_1:83340760-83340779 None:intergenic 20.0%
!! TCATATTTACTAAAGACAAT+CGG + chr8_1:83340787-83340806 None:intergenic 20.0%
!!! AAGGAAATTTTTGAATTCAA+AGG - chr8_1:83340872-83340891 Msa1220280:CDS 20.0%
!!! CAATGATTTTATTGCAAAAT+TGG - chr8_1:83340983-83341002 Msa1220280:intron 20.0%
!!! TGTACGTTTTGTATTTTAAA+AGG - chr8_1:83341121-83341140 Msa1220280:intron 20.0%
!!! GTATTTTAAAAGGAAACTTT+AGG - chr8_1:83341131-83341150 Msa1220280:intron 20.0%
!! TATATAATATCTCACTAAGT+AGG + chr8_1:83341546-83341565 None:intergenic 20.0%
!! ACAAAACATTTATGCATAAA+AGG - chr8_1:83341568-83341587 Msa1220280:intron 20.0%
!!! CTTTTTGATGTTAAGAAAAA+AGG - chr8_1:83341615-83341634 Msa1220280:intron 20.0%
!! GTTAATATTTGAAAAGAGAT+AGG + chr8_1:83341785-83341804 None:intergenic 20.0%
!! AATTAAAAACATAGAATGAG+AGG - chr8_1:83341849-83341868 Msa1220280:intron 20.0%
!! TTGCATTATTAATAACTGAT+AGG - chr8_1:83342280-83342299 Msa1220280:intron 20.0%
!! AAAGTTTCAATCTGATAAAT+AGG - chr8_1:83342305-83342324 Msa1220280:intron 20.0%
!! TAGCTTAATATCTATGTTTA+AGG + chr8_1:83342505-83342524 None:intergenic 20.0%
! AAACTCGAAAAAACATAGAA+AGG - chr8_1:83340191-83340210 Msa1220280:three_prime_UTR 25.0%
! AACTCGAAAAAACATAGAAA+GGG - chr8_1:83340192-83340211 Msa1220280:three_prime_UTR 25.0%
!! TTTTCAATCACTGTAATTGT+TGG - chr8_1:83340500-83340519 Msa1220280:three_prime_UTR 25.0%
! CAATATATCAAACAGATGAT+AGG + chr8_1:83340559-83340578 None:intergenic 25.0%
! AAAGCTATAAAAGATGTGTT+AGG + chr8_1:83340582-83340601 None:intergenic 25.0%
! AGTTGGACAAAATTTCAATT+AGG + chr8_1:83341101-83341120 None:intergenic 25.0%
! AAAATACAAAACGTACAAGT+TGG + chr8_1:83341118-83341137 None:intergenic 25.0%
! ACAAAAAAACATCTTTCAAC+TGG + chr8_1:83341179-83341198 None:intergenic 25.0%
! AATATAACCGTAAGTACTAT+CGG + chr8_1:83341444-83341463 None:intergenic 25.0%
!! CCTTTTTATAGCTATTATAC+AGG + chr8_1:83341827-83341846 None:intergenic 25.0%
! CCTGTATAATAGCTATAAAA+AGG - chr8_1:83341824-83341843 Msa1220280:intron 25.0%
! GAATTATCAAATACGTATCA+TGG + chr8_1:83341904-83341923 None:intergenic 25.0%
TCCTCCAAACACAATAAAAA+TGG + chr8_1:83340246-83340265 None:intergenic 30.0%
AAGTTGACGAGAAATAAGAA+TGG + chr8_1:83340465-83340484 None:intergenic 30.0%
TCTCGTCAACTTATGATTAA+TGG - chr8_1:83340473-83340492 Msa1220280:three_prime_UTR 30.0%
CTCGTCAACTTATGATTAAT+GGG - chr8_1:83340474-83340493 Msa1220280:three_prime_UTR 30.0%
!! TTGTCTTTGTTCGAGTTTTT+GGG - chr8_1:83340919-83340938 Msa1220280:intron 30.0%
ACATTCATTTCGTGAGAAAA+GGG + chr8_1:83341023-83341042 None:intergenic 30.0%
AACATTCATTTCGTGAGAAA+AGG + chr8_1:83341024-83341043 None:intergenic 30.0%
! ATCGGAGAATTTGTACTTTT+TGG - chr8_1:83341070-83341089 Msa1220280:intron 30.0%
CAACAATGTAGTTTAGTGAT+TGG - chr8_1:83341228-83341247 Msa1220280:intron 30.0%
! AACCGTATCAACTCTTTTAA+GGG + chr8_1:83341360-83341379 None:intergenic 30.0%
! AAACCGTATCAACTCTTTTA+AGG + chr8_1:83341361-83341380 None:intergenic 30.0%
AATATCTCACTAAGTAGGAT+TGG + chr8_1:83341541-83341560 None:intergenic 30.0%
TAACATCAAAAAGCCTAGAA+GGG + chr8_1:83341608-83341627 None:intergenic 30.0%
TTAACATCAAAAAGCCTAGA+AGG + chr8_1:83341609-83341628 None:intergenic 30.0%
!! GTTAAGAAAAAAGGTGTCAA+TGG - chr8_1:83341624-83341643 Msa1220280:intron 30.0%
TACAAAAGATTTACCAAGAG+AGG + chr8_1:83341726-83341745 None:intergenic 30.0%
TTATAGCTATTATACAGGCT+TGG + chr8_1:83341822-83341841 None:intergenic 30.0%
CCAAGAAATCTTCGTAATTT+AGG + chr8_1:83342058-83342077 None:intergenic 30.0%
CCTAAATTACGAAGATTTCT+TGG - chr8_1:83342055-83342074 Msa1220280:intron 30.0%
GAAAAGAAAAGTGAAACCAA+AGG - chr8_1:83342160-83342179 Msa1220280:intron 30.0%
TCAAGAGGAAGTTAAAACTT+GGG + chr8_1:83342257-83342276 None:intergenic 30.0%
TTAATAATGCAAGAGTCAAG+AGG + chr8_1:83342272-83342291 None:intergenic 30.0%
!!! GTTCATTTTTTTTGCAGAAC+AGG - chr8_1:83342359-83342378 Msa1220280:intron 30.0%
ATGGTTACAAGAATGTAGTT+AGG + chr8_1:83342453-83342472 None:intergenic 30.0%
TCTTCATGTGATATATCTGT+TGG - chr8_1:83342626-83342645 Msa1220280:five_prime_UTR 30.0%
!!! CACGCCATTTTTATTGTGTT+TGG - chr8_1:83340239-83340258 Msa1220280:three_prime_UTR 35.0%
!!! GCCATTTTTATTGTGTTTGG+AGG - chr8_1:83340242-83340261 Msa1220280:three_prime_UTR 35.0%
! TAACGATGATGATGATCACT+GGG + chr8_1:83340296-83340315 None:intergenic 35.0%
! ATAACGATGATGATGATCAC+TGG + chr8_1:83340297-83340316 None:intergenic 35.0%
ACGAGAAATAAGAATGGAGA+TGG + chr8_1:83340459-83340478 None:intergenic 35.0%
AACTTTGCAAGAAACCATTC+AGG - chr8_1:83340638-83340657 Msa1220280:CDS 35.0%
! TAATTGATTGATGGTTGTGC+CGG - chr8_1:83340738-83340757 Msa1220280:CDS 35.0%
! AGTGTTTCTGTCACATCATT+TGG - chr8_1:83340840-83340859 Msa1220280:CDS 35.0%
CATCATTTGGCATTCAAGAA+AGG - chr8_1:83340853-83340872 Msa1220280:CDS 35.0%
AAGGTGATGCAGTTGAATTA+TGG - chr8_1:83340891-83340910 Msa1220280:CDS 35.0%
!! CTTGTCTTTGTTCGAGTTTT+TGG - chr8_1:83340918-83340937 Msa1220280:intron 35.0%
!! TGTCTTTGTTCGAGTTTTTG+GGG - chr8_1:83340920-83340939 Msa1220280:intron 35.0%
! TCTTTCAACTGGCTTAACAA+CGG + chr8_1:83341168-83341187 None:intergenic 35.0%
GGGACAAAATCATCAGTAAT+TGG + chr8_1:83341340-83341359 None:intergenic 35.0%
!! GTCCCTTAAAAGAGTTGATA+CGG - chr8_1:83341355-83341374 Msa1220280:intron 35.0%
AGTTTGACCGATAGTACTTA+CGG - chr8_1:83341434-83341453 Msa1220280:intron 35.0%
ACCTACAATATACTGATGTG+TGG - chr8_1:83341692-83341711 Msa1220280:intron 35.0%
CCTACAATATACTGATGTGT+GGG - chr8_1:83341693-83341712 Msa1220280:intron 35.0%
AATATACTGATGTGTGGGAA+TGG - chr8_1:83341698-83341717 Msa1220280:intron 35.0%
TTCTGTTTGCAAGTGCAAAA+CGG - chr8_1:83341931-83341950 Msa1220280:intron 35.0%
!! AACAACGTGAAGTTTTCAAG+TGG - chr8_1:83342126-83342145 Msa1220280:intron 35.0%
!!! TGAAGTTTTCAAGTGGATTC+TGG - chr8_1:83342133-83342152 Msa1220280:intron 35.0%
AGGAAGTTAAAACTTGGGTA+TGG + chr8_1:83342252-83342271 None:intergenic 35.0%
GTCAAGAGGAAGTTAAAACT+TGG + chr8_1:83342258-83342277 None:intergenic 35.0%
!! TTCCAACAGTTTTAGCCTTT+AGG - chr8_1:83342408-83342427 Msa1220280:intron 35.0%
!! TCCAACAGTTTTAGCCTTTA+GGG - chr8_1:83342409-83342428 Msa1220280:intron 35.0%
CAATAAAAATGGCGTGAGTG+TGG + chr8_1:83340235-83340254 None:intergenic 40.0%
! ACGAATTTGAAGCGCTTCAT+GGG + chr8_1:83340375-83340394 None:intergenic 40.0%
! AACGAATTTGAAGCGCTTCA+TGG + chr8_1:83340376-83340395 None:intergenic 40.0%
! TGAAGCGCTTCAAATTCGTT+TGG - chr8_1:83340376-83340395 Msa1220280:three_prime_UTR 40.0%
TTGCTTTCCAATCTTGCACT+TGG - chr8_1:83340408-83340427 Msa1220280:three_prime_UTR 40.0%
GACAAGGAAACAACACATTG+TGG + chr8_1:83340526-83340545 None:intergenic 40.0%
CGTGTCTTTATTAAGCTAGC+AGG - chr8_1:83340608-83340627 Msa1220280:CDS 40.0%
ATGTAACTGCATGCTGTGAT+CGG - chr8_1:83341052-83341071 Msa1220280:intron 40.0%
! TTTCAACTGGCTTAACAACG+GGG + chr8_1:83341166-83341185 None:intergenic 40.0%
! CTTTCAACTGGCTTAACAAC+GGG + chr8_1:83341167-83341186 None:intergenic 40.0%
TAACACCATACTTTGCAGTC+TGG - chr8_1:83341266-83341285 Msa1220280:intron 40.0%
!! CTAAGTAGGATTGGCTACAT+GGG + chr8_1:83341532-83341551 None:intergenic 40.0%
!! ACTAAGTAGGATTGGCTACA+TGG + chr8_1:83341533-83341552 None:intergenic 40.0%
CCCACACATCAGTATATTGT+AGG + chr8_1:83341696-83341715 None:intergenic 40.0%
TGCAAAACGGAGAGATTCAA+TGG - chr8_1:83341944-83341963 Msa1220280:intron 40.0%
AGTGAAACCAAAGGATGCTA+CGG - chr8_1:83342169-83342188 Msa1220280:intron 40.0%
! TTTTTCTCCGTAGCATCCTT+TGG + chr8_1:83342179-83342198 None:intergenic 40.0%
TTTGCAGAACAGGACAGTAA+CGG - chr8_1:83342369-83342388 Msa1220280:intron 40.0%
TCCCTAAAGGCTAAAACTGT+TGG + chr8_1:83342413-83342432 None:intergenic 40.0%
TTTGATGCTTGCTTCCCTAA+AGG + chr8_1:83342426-83342445 None:intergenic 40.0%
GTTATCCAACAATGCACGAT+TGG + chr8_1:83342477-83342496 None:intergenic 40.0%
AAAAGTAGAGACCTACCAAG+CGG + chr8_1:83342581-83342600 None:intergenic 40.0%
CTTTCCAATCTTGCACTTGG+AGG - chr8_1:83340411-83340430 Msa1220280:three_prime_UTR 45.0%
GATAGGCAAAATGCGTGACA+AGG + chr8_1:83340542-83340561 None:intergenic 45.0%
! TTGATGGTTGTGCCGGTAAA+AGG - chr8_1:83340745-83340764 Msa1220280:CDS 45.0%
!! TGTTCGAGTTTTTGGGGACA+TGG - chr8_1:83340926-83340945 Msa1220280:intron 45.0%
!! GTTCGAGTTTTTGGGGACAT+GGG - chr8_1:83340927-83340946 Msa1220280:intron 45.0%
! TTCAACTGGCTTAACAACGG+GGG + chr8_1:83341165-83341184 None:intergenic 45.0%
ACCATACTTTGCAGTCTGGT+GGG - chr8_1:83341270-83341289 Msa1220280:intron 45.0%
GTAGAGACAGTGACTTCACA+TGG + chr8_1:83341318-83341337 None:intergenic 45.0%
CTAGAAGGGTTGAGAAGTTG+AGG + chr8_1:83341594-83341613 None:intergenic 45.0%
CTCAACTTCTCAACCCTTCT+AGG - chr8_1:83341592-83341611 Msa1220280:intron 45.0%
! CGTTGTTGTTCGTCAGGAAT+TGG + chr8_1:83342113-83342132 None:intergenic 45.0%
! ACTTCACGTTGTTGTTCGTC+AGG + chr8_1:83342119-83342138 None:intergenic 45.0%
TTGCAGAACAGGACAGTAAC+GGG - chr8_1:83342370-83342389 Msa1220280:intron 45.0%
!! ACAGGACAGTAACGGGTTAA+TGG - chr8_1:83342377-83342396 Msa1220280:intron 45.0%
CCAACAATGCACGATTGGTA+TGG + chr8_1:83342472-83342491 None:intergenic 45.0%
CCATACCAATCGTGCATTGT+TGG - chr8_1:83342469-83342488 Msa1220280:CDS 45.0%
AAAGTAGAGACCTACCAAGC+GGG + chr8_1:83342580-83342599 None:intergenic 45.0%
TTCATGGGTAACCCAGTTGG+AGG + chr8_1:83340360-83340379 None:intergenic 50.0%
CTGACCTCCAAGTGCAAGAT+TGG + chr8_1:83340418-83340437 None:intergenic 50.0%
!! GAGTTTTTGGGGACATGGGA+AGG - chr8_1:83340931-83340950 Msa1220280:intron 50.0%
!! GACATGGGAAGGAGCATCTA+CGG - chr8_1:83340942-83340961 Msa1220280:intron 50.0%
!! ACATGGGAAGGAGCATCTAC+GGG - chr8_1:83340943-83340962 Msa1220280:intron 50.0%
CACCATACTTTGCAGTCTGG+TGG - chr8_1:83341269-83341288 Msa1220280:intron 50.0%
CCATACTTTGCAGTCTGGTG+GGG - chr8_1:83341271-83341290 Msa1220280:intron 50.0%
TGTGGGAATGGTTCCTCTCT+TGG - chr8_1:83341710-83341729 Msa1220280:intron 50.0%
ACTGAACTTGTGCTGGGTTC+AGG + chr8_1:83342032-83342051 None:intergenic 50.0%
TGAACCCAGCACAAGTTCAG+TGG - chr8_1:83342031-83342050 Msa1220280:intron 50.0%
AGGTCCACTGAACTTGTGCT+GGG + chr8_1:83342038-83342057 None:intergenic 50.0%
TAGGTCCACTGAACTTGTGC+TGG + chr8_1:83342039-83342058 None:intergenic 50.0%
! ACTTCCATAGGCATTGTGGC+AGG + chr8_1:83342083-83342102 None:intergenic 50.0%
CCACAATGCCTATGGAAGTG+CGG - chr8_1:83342084-83342103 Msa1220280:intron 50.0%
! CACAATGCCTATGGAAGTGC+GGG - chr8_1:83342085-83342104 Msa1220280:intron 50.0%
TGGGTATGGAATTTGCACGC+AGG + chr8_1:83342238-83342257 None:intergenic 50.0%
TGAGAACCATTATGCCCGCT+TGG - chr8_1:83342563-83342582 Msa1220280:five_prime_UTR 50.0%
AACCATTATGCCCGCTTGGT+AGG - chr8_1:83342567-83342586 Msa1220280:five_prime_UTR 50.0%
CGCTTCATGGGTAACCCAGT+TGG + chr8_1:83340363-83340382 None:intergenic 55.0%
CCCCACCAGACTGCAAAGTA+TGG + chr8_1:83341274-83341293 None:intergenic 55.0%
GAACTTGTGCTGGGTTCAGG+AGG + chr8_1:83342029-83342048 None:intergenic 55.0%
GGTTCCTGCCACAATGCCTA+TGG - chr8_1:83342076-83342095 Msa1220280:intron 55.0%
! CCGCACTTCCATAGGCATTG+TGG + chr8_1:83342087-83342106 None:intergenic 55.0%
ATTGGAGCCCGCACTTCCAT+AGG + chr8_1:83342095-83342114 None:intergenic 55.0%
GACCTACCAAGCGGGCATAA+TGG + chr8_1:83342572-83342591 None:intergenic 55.0%
CACCACCTGCACCTCCAACT+GGG - chr8_1:83340346-83340365 Msa1220280:three_prime_UTR 60.0%
! AACCCAGTTGGAGGTGCAGG+TGG + chr8_1:83340351-83340370 None:intergenic 60.0%
! GGTAACCCAGTTGGAGGTGC+AGG + chr8_1:83340354-83340373 None:intergenic 60.0%
! GTTGGAGGTGCAGGTGGTGG+TGG + chr8_1:83340345-83340364 None:intergenic 65.0%
! CCAGTTGGAGGTGCAGGTGG+TGG + chr8_1:83340348-83340367 None:intergenic 65.0%
CCACCACCTGCACCTCCAAC+TGG - chr8_1:83340345-83340364 Msa1220280:three_prime_UTR 65.0%
!! GGAGGTGCAGGTGGTGGTGG+AGG + chr8_1:83340342-83340361 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 83340186 83342660 83340186 ID=Msa1220280;Name=Msa1220280
chr8_1 mRNA 83340186 83342660 83340186 ID=Msa1220280-mRNA-1;Parent=Msa1220280;Name=Msa1220280-mRNA-1;_AED=0.00;_eAED=0.00;_QI=141|1|1|1|1|1|2|395|147
chr8_1 exon 83340186 83340918 83340186 ID=Msa1220280-mRNA-1:exon:29893;Parent=Msa1220280-mRNA-1
chr8_1 exon 83342414 83342660 83342414 ID=Msa1220280-mRNA-1:exon:29892;Parent=Msa1220280-mRNA-1
chr8_1 five_prime_UTR 83342520 83342660 83342520 ID=Msa1220280-mRNA-1:five_prime_utr;Parent=Msa1220280-mRNA-1
chr8_1 CDS 83342414 83342519 83342414 ID=Msa1220280-mRNA-1:cds;Parent=Msa1220280-mRNA-1
chr8_1 CDS 83340581 83340918 83340581 ID=Msa1220280-mRNA-1:cds;Parent=Msa1220280-mRNA-1
chr8_1 three_prime_UTR 83340186 83340580 83340186 ID=Msa1220280-mRNA-1:three_prime_utr;Parent=Msa1220280-mRNA-1
Gene Sequence

>Msa1220280

ATGAATGAGACTCCTCCACCACCACCTGCACCTCCAACTGGGTTACCCATGAAGCGCTTCAAATTCGTTTGGCGCTTTCTCTTGCTTTCCAATCTTGCACTTGGAGCTTTTCTGTTTGCAAGTGCAAAACGGAGAGATTCAATGGAGATTCAGAAAAAAAGAACAGCACACAGATTGCATAAGAGCAAAGCTGCTGTCGAAGTTCCTCCTGAACCCAGCACAAGTTCAGTGGACCTAAATTACGAAGATTTCTTGGTTCCTGCCACAATGCCTATGGAAGTGCGGGCTCCAATTCCTGACGAACAACAACGTGAAGTTTTCAAGTGGATTCTGGAAGAGAAAAGAAAAGTGAAACCAAAGGATGCTACGGAGAAAAAACAAATTGACGAAGACAAGTACATTCTTAAGCAGTTCCTGCGTGCAAATTCCATACCCAAGTTTTAA
Protein sequence

>Msa1220280

MNETPPPPPAPPTGLPMKRFKFVWRFLLLSNLALGAFLFASAKRRDSMEIQKKRTAHRLHKSKAAVEVPPEPSTSSVDLNYEDFLVPATMPMEVRAPIPDEQQREVFKWILEEKRKVKPKDATEKKQIDEDKYILKQFLRANSIPKF*